Abstract
pANCA and ASCA are useful in confirming the diagnosis of IBD and differentiating between UC and CD in an IBD cohort in central China.
Copyright 2010 Elsevier B.V. All rights reserved....
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PMID: 20570669
PDF is available here.
Abstract
The results evidenced that low sensitivity of ASCA and pANCA markers limits their use in IBD screening in the general population; however, their specificity may contribute to differentiation between CD and UC in IBD patients. Our study lends further support to the suggestion that serologic assessmen...
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PMID: 19826949
PDF is available here.
Abstract
We analyzed GalXM from acapsular serotype A and D strains by carbohydrate analysis and static and dynamic light scattering to determine mass, effective diameter, polydispersity, and diffusion coefficients. Multiangle laser light scattering showed that GalXM from C. neoformans var. grubii strain cap5...
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PMID: 20061411
PDF is available here.
Abstract
We failed in our initial effort to purify a prematurely developed Camelid-like E-tagged short scFv-K2 antibody that contained a complete variable region of the heavy chain and partial region of the light chain by using an anti-E-tag affinity column. To expedite the purification of this altered but i...
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PMID: 20060473
PDF is available here.
Abstract
We investigated an outbreak of coccidioidomycosis in a 12-person civilian construction crew that excavated soil during an underground pipe installation on Camp Roberts Military Base, California in October 2007. Ten (83.3%) workers developed symptoms of coccidioidomycosis; eight (66.7%) had serologic...
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PMID: 19845993
PDF is available here.
Abstract
There is some evidence that dogs can be naturally infected by Paracoccidioides brasiliensis in endemic areas of paracoccidioidomycosis. In order to evaluate canine infection with this fungus, a survey with 149 urban and 126 rural dogs was carried out using ELISA and intradermal tests...
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PMID: 19768574
PDF is available here.
Abstract
There is some evidence that dogs can be naturally infected by Paracoccidioides brasiliensis in endemic areas of paracoccidioidomycosis. In order to evaluate canine infection with this fungus, a survey with 149 urban and 126 rural dogs was carried out using ELISA and intradermal tests...
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PMID: 19768574
PDF is available here.
Abstract
There is some evidence that dogs can be naturally infected by Paracoccidioides brasiliensis in endemic areas of paracoccidioidomycosis. In order to evaluate canine infection with this fungus, a survey with 149 urban and 126 rural dogs was carried out using ELISA and intradermal tests...
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PMID: 19768574
PDF is available here.
Abstract
There is some evidence that dogs can be naturally infected by Paracoccidioides brasiliensis in endemic areas of paracoccidioidomycosis. In order to evaluate canine infection with this fungus, a survey with 149 urban and 126 rural dogs was carried out using ELISA and intradermal tests...
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PMID: 19768574
PDF is available here.
Abstract
There is some evidence that dogs can be naturally infected by Paracoccidioides brasiliensis in endemic areas of paracoccidioidomycosis. In order to evaluate canine infection with this fungus, a survey with 149 urban and 126 rural dogs was carried out using ELISA and intradermal tests...
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PMID: 19768574
PDF is available here.
Abstract
There is some evidence that dogs can be naturally infected by Paracoccidioides brasiliensis in endemic areas of paracoccidioidomycosis. In order to evaluate canine infection with this fungus, a survey with 149 urban and 126 rural dogs was carried out using ELISA and intradermal tests...
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PMID: 19768574
PDF is available here.
Abstract
There is some evidence that dogs can be naturally infected by Paracoccidioides brasiliensis in endemic areas of paracoccidioidomycosis. In order to evaluate canine infection with this fungus, a survey with 149 urban and 126 rural dogs was carried out using ELISA and intradermal tests...
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PMID: 19768574
PDF is available here.
Abstract
There is some evidence that dogs can be naturally infected by Paracoccidioides brasiliensis in endemic areas of paracoccidioidomycosis. In order to evaluate canine infection with this fungus, a survey with 149 urban and 126 rural dogs was carried out using ELISA and intradermal tests...
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PMID: 19768574
PDF is available here.
Abstract
There is some evidence that dogs can be naturally infected by Paracoccidioides brasiliensis in endemic areas of paracoccidioidomycosis. In order to evaluate canine infection with this fungus, a survey with 149 urban and 126 rural dogs was carried out using ELISA and intradermal tests...
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PMID: 19768574
PDF is available here.
Abstract
There is some evidence that dogs can be naturally infected by Paracoccidioides brasiliensis in endemic areas of paracoccidioidomycosis. In order to evaluate canine infection with this fungus, a survey with 149 urban and 126 rural dogs was carried out using ELISA and intradermal tests...
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PMID: 19768574
PDF is available here.
Abstract
There is some evidence that dogs can be naturally infected by Paracoccidioides brasiliensis in endemic areas of paracoccidioidomycosis. In order to evaluate canine infection with this fungus, a survey with 149 urban and 126 rural dogs was carried out using ELISA and intradermal tests...
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PMID: 19768574
PDF is available here.
Abstract
There is some evidence that dogs can be naturally infected by Paracoccidioides brasiliensis in endemic areas of paracoccidioidomycosis. In order to evaluate canine infection with this fungus, a survey with 149 urban and 126 rural dogs was carried out using ELISA and intradermal tests...
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PMID: 19768574
PDF is available here.
Abstract
The aim of our study was to evaluate the sensitivity, specificity and predictive values of isolated and combined ASCA and NANA assays in Tunisian inflammatory bowel diseases patients and to assess whether their presence is associated with particular clinical features of the disease. Methods: 128 pa...
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PMID: 20870580
PDF is available here.
Abstract
The major diagnostic groups included patients with bronchogenic carcinoma (n = 31) and tubercular sequelae (n = 16). In all, 28 patients (46.7%) were culture-positive, with Candida and Aspergillus being recovered from 14 and 13 patients respectively. Twenty-one patients (35%) showed presence of prec...
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PMID: 20854652
PDF is available here.
Abstract
We previously reported the presence of an antibody which reacts to beta-glucan, anti-beta-glucan (BG) antibody, in human sera. In livestock and domestic pets, the antibody's response to fungal cell wall beta-glucan is little understood. In this study, we examined the existence and reactivity of anti...
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PMID: 20467198
PDF is available here.
Abstract
There is evidence for the immunomodulation disorders in the response to intestinal flora in inflammatory bowel disease, however, the role of yeasts in the aetiopathogenesis of ulcerative colitis has not been fully clarified. The aim of this study was to assess the serum concentration...
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PMID: 19758974
PDF is available here.
Abstract
Neospora caninum is an important cause of abortion in dairy cattle worldwide. Dog is the definitive host for N. caninum and can infect dairy cattle. The aim of this study is to determine the prevalence of Neospora oocysts in feces of dogs from dairy farms. A total of 174 fecal samples was collected...
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PMID: 19967094
PDF is available here.
Abstract
To evaluate immunogenicity of the recombinant protein GST-p55/570 of Pneumocystis carinii.
The fusion protein GST-p55/570 was expressed from the prokaryotic expression plasmid pGEX-570, and purified by using glutathione-agarose. The expressed product was analyzed by SDS-PAGE. Thirty-three mice were...
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PMID: 20232632
PDF is available here.
Drugs (69)Supplement 1 2009
Abstract
A diagnosis of invasive candidiasis can be achieved using conventional approaches (microscopy, culture, serology), as well as new methods, including antigen detection and polymerase chain reaction (PCR) assays. Most of the conventional approaches lack sensitivity, especially for obtaining a diagnosi...
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PMID: 19877736
PDF is available here.
Abstract
We prepared a polyclonal anti-Candida albicans antibody in chicken egg yolk (anti-C. albicans IgY) and investigated its in vitro effectiveness in preventing C. albicans adherence and biofilm formation. Anti-C. albicans IgY significantly reduced the adherence of C. albicans SC5314 to human oral epith...
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PMID: 19762981
PDF is available here.
Maria Papp,
Ildiko Foldi,
Istvan Altorjay,
Eszter Palyu,
Miklos Udvardy,
Judit Tumpek,
Sandor Sipka,
Ilma Rita Korponay-Szabo,
Eva Nemes,
Gabor Veres,
Tamas Dinya,
Attila Tordai,
Hajnalka Andrikovics,
Gary L Norman and
Peter Laszlo Lakatos
Abstract
65.9% of the CD patients were positive for at least one of the tested antibodies at the time of the diagnosis. Except anti-OMP and ALCA, anti-microbial antibodies were exclusively seen in untreated CD; however, the overall sensitivity was low. Any glycan positivity (LR+: 3.13; 95% CI: 2.08-4.73) was...
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PMID: 19701969
PDF is available here.
Abstract
We present the case of a man with suberosis caused by Mucor species, a mold that has been rarely reported to cause hypersensitivity pneumonitis. Furthermore, the patient had symptoms and lung function tests indicating bronchial obstruction-an atypical presentation of hypersensitivity pneumonitis. No...
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PMID: 19376624
PDF is available here.
Abstract
We report the first case of Coccidioides immitis meningitis in a patient with HIES. Coccidioides should be included in the differential diagnosis for central nervous system infections in HIES patients....
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PMID: 19483664
PDF is available here.
Abstract
Two monoclonal antibodies (MAbs) were raised against the Candida albicans cell-surface glycoprotein Als3 using the N-terminal domain of the protein as the immunogen. ELISA was used to demonstrate the specificity of the MAbs for the Als3 fragment, but not for the corresponding N-terminal domain fragm...
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PMID: 19427882
PDF is available here.
Abstract
We describe here the principle of this serological analysis and its perspectives related to the use of multianalyte profiling technology for a a better understanding of IC and CD pathophysiology. This may contribute to improve disease management in terms of diagnosis and therapy....
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PMID: 19480828
PDF is available here.
Abstract
The frequency of the mutation was significantly higher in Crohn's disease than in control (p = 0,0005; OR = 20.45; CI 95% = 2.86-413.85) and did not differ statistically in UC group (p = 0, 05) from control. ASCAs were present in 60% of CD and 20, 8% of UC. CONCLUSION: This study suggests that in no...
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PMID: 19184350
PDF is available here.
Abstract
Coccidioidomycosis is a systemic infection caused by Coccidioides spp. The disease is endemic in Brazil but its incidence is underreported as it is not a notifiable disease. This article presents the results of a serologic survey carried out with 229 volunteers in northeast Brazil by the immunodiffu...
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PMID: 19116771
PDF is available here.
Abstract
Important advances are being reached in the use of biomarkers for the diagnosis of invasive candidiasis, and some of them are being used to start a preemptive therapy strategy....
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PMID: 19463272
PDF is available here.
Abstract
We generated immunoglobulin G (IgG) isotype monoclonal antibodies (MAbs) to H. capsulatum Hsp60. IgG1 and IgG2a MAbs significantly prolonged the survival of mice infected with H. capsulatum. An IgG2b MAb was not protective. The protective MAbs reduced intracellular fungal survival and increased phag...
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PMID: 19179416
PDF is available here.
RK Russell,
B Ip,
MC Aldhous,
M MacDougall,
HE Drummond,
IDR Arnott,
PM Gillett,
P McGrogan,
LT Weaver,
WM Bisset,
G Mahdi,
DC Wilson and
J Satsangi
Abstract
Patients with CD had a higher prevalence of ASCA than did other patients with IBD. ASCA status described patients with CD who had a specific phenotype, showing an association with markers of disease severity and oral CD involvement....
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PMID: 19179877
PDF is available here.
Abstract
The specificity of these combined markers tends to be higher than sensitivity, what made them more useful in the differentiation of the IBD subtypes rather than population screening. The characteristic IC serotype pANCA(-)ASCA(-) leads to further controversies about origin of this IBD subtype....
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PMID: 19453050
PDF is available here.
Dermot P B McGovern,
Kent D Taylor,
Carol Landers,
Carrie Derkowski,
Deb Dutridge,
Marla Dubinsky,
Andy Ippoliti,
Eric Vasiliauskas,
Ling Mei,
Emebet Mengesha,
Lily King,
Sheila Pressman,
Stephan R Targan and
Jerome I Rotter
Abstract
These findings support the significance of the epithelial barrier in IBD pathogenesis....
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PMID: 18720471
PDF is available here.
Abstract
The measurement of antibodies in the external secretions that bathe mucosal surfaces is important in understanding the host response to the opportunistic pathogen, Candida albicans and its determinants of pathogenesis at these sites. The principal immunoglobulin isotype in mucosal secretions is secr...
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PMID: 19152034
PDF is available here.
Abstract
We describe the typical proteomic strategy to globally and integratively profile these host antibody responses and determine serum antibody signatures. This approach is based on the combination of classic immunoproteomics or serologic proteome analysis (two-dimensional electrophoresis followed by qu...
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PMID: 19089396
PDF is available here.
Abstract
We describe methods used in our lab, in which sera recovered from patients with candidiasis are used to screen a C. albicans genomic DNA expression library. Immunoreactive colonies are detected by reaction with anti-human immunoglobulin, and the corresponding open reading frames are identified using...
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PMID: 19089384
PDF is available here.
Abstract
We describe here represents a useful tool to investigate to what extent yeasts are able to regulate their glycan surface expression and therefore modify their virulence properties....
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PMID: 19089378
PDF is available here.
Abstract
Systemic candidiasis is a significant cause of morbidity and mortality in patients with hematologic disorders. The aim of this study was to determine the prevalence of systemic candidiasis and the efficiency of the polymerase chain reaction-enzyme-linked immunosorbent assay (PCR-ELIZA) method for th...
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PMID: 19168950
PDF is available here.
Abstract
Our previous studies as well as different studies from other countries have shown that A. alternata is one of the most common indoor and outdoor airborne fungi, so it could permanently present some allergens to susceptible individuals. Therefore, control of A. alternata growth in indoor areas and av...
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PMID: 19673169
PDF is available here.
Abstract
Here, we provide evidence that the malate synthase of P. brasiliensis (PbMLS) is located on the fungal cell surface, and is secreted. PbMLS was overexpressed in Escherichia coli, and polyclonal antibody was obtained against this protein. By using Confocal Laser Scanning Microscopy, PbMLS was detecte...
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PMID: 20034376
PDF is available here.
Abstract
We showed that the pathogenic fungus Cryptococcus neoformans (Cn) produces a specific and unique protein called antiphagocytic protein 1 (App1), which inhibits phagocytosis of Cn by alveolar macrophages (AMs). Phagocytosis of Cn by AMs occurs mainly through a complement- or Ab-mediated mechanism. Am...
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PMID: 19109138
PDF is available here.
Abstract
We described against yeast oligomannosides (ASCA). This prompted us to investigate a role for another yeast, Candida albicans, a very common commensal of the human digestive tract and an important opportunistic pathogen. CLINICAL AND EXPERIMENTAL DATA: It has been revealed that the major oligomannos...
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PMID: 20203505
PDF is available here.
Abstract
The diagnostic and therapeutic approaches to IFI have changed significantly in recent years, fostered by the introduction of new diagnostic methods and new antifungal products. The diagnosis of invasive candidiasis (IC) involves both clinical and laboratory parameters, but neither of these are speci...
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PMID: 19071886
PDF is available here.
Abstract
We used a two-step chromatography procedure to purify and identify the antigen (3D9) from C. albicans strain 66396 germ tubes. MAb 3D9.3 recognized two intense protein bands at 140 and 180 kDa. A comparative analysis between theoretical and experimental mass spectrum peaks showed that both bands cor...
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PMID: 19291169
PDF is available here.
Abstract
We suggested the utility of anti-saccharomyces cerevisiae antibody (ASCA) and anti-neutrophilic cytoplasmic antibody (p-ANCA) in the differential diagnosis of BD from IBD....
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PMID: 19084948
PDF is available here.
Abstract
The Candida antigen CR3-RP (complement receptor 3-related protein) is supposed to be a 'mimicry' protein because of its ability to bind antibody directed against the alpha subunit of the mammalian CR3 (CD11b/CD18). This study aimed to (i) investigate the specific humoral isotypic response to immuniz...
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PMID: 19018015
PDF is available here.
Abstract
Infection with Encephalitozoon cuniculi in rabbits frequently exists as a chronic, latent infection, and only a percentage of infected animals develop clinical disease. This study presents a seroepidemiological study of E. cunicucli infection in 337 pet rabbits collected from 20 prefectures in Japan...
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PMID: 19122395
PDF is available here.
Abstract
The present study was designed to evaluate the efficiency of two serodiagnostic tests; the direct agglutination test (DAT) and the fast agglutination screening test (FAST) in the diagnosis of Microsporidia in experimentally infected mice and to differentiate between different species of the parasite...
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PMID: 19209773
PDF is available here.
Abstract
We found no Hp 1-1 expression. The role of Hp molecules may be explained by their distinct immunomodulatory properties and structural characteristics. Sero-reactivity to microbial components or perinuclear components of neutrophils (atypical P-ANCA) is reported to be associated with disease phenotyp...
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PMID: 19028649
PDF is available here.
Abstract
Allergic bronchopulmonary aspergillosis (ABPA) is seen in approximately 10% of patients with cystic fibrosis (CF) and can be difficult to diagnose. Consensus criteria require the presence of multiple elevated immunologic markers such as total immunoglobulin E (IgE), Aspergillus IgE and Aspergillus I...
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PMID: 18947811
PDF is available here.
Abstract
We encountered a family in which two of four members, the husband and his wife, had summer-type hypersensitivity pneumonitis at the same time, about two months after they moved to the residence. A 45-year-old man had cough, fever and exertional dyspnea. Chest computed tomography showed diffuse centr...
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PMID: 19052513
PDF is available here.
Abstract
We show that a dominant IgE epitope on the N-terminally processed Pen ch 18 allergen was narrowed down to residues 83-91. In addition, Lys89, Lys90, and possibly Phe91 were identified as the core residues. Substitution of Lys89, Lys90, or Phe91 with alanine can significantly reduce IgE-binding to Pe...
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PMID: 18760997
PDF is available here.