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Antibodies, Helminth (0):
Recent article
Antibodies, Helminth
Abstract
Human toxocariasis is a worldwide parasitic disease. Children are more frequently infected because of the closer contact with contaminated soil and relatively frequent geophagia. Toxocariasis in children has variable modes of presentation but clinical diagnosis is difficult. Various...
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PMID: 21980766
PDF is available here.
PDF is available here.
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Abstract
We have found associations between fitness and heritable self-reactive antibody responsiveness in a wild population of Soay sheep. The occurrence of self-reactive antibodies correlated with overall antibody responsiveness and was associated with reduced reproduction in adults of both sexes. However,...
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PMID: 21030656
PDF is available here.
PDF is available here.
Abstract
Neurocysticercosis (NC), an infection of the CNS with Taenia solium metacestode, exemplifies formidable public health concerns associated with significant morbidity and mortality. The disease is a complex phenomenon involving molecular cell biological cross-talks between the parasite and human host....
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PMID: 20973642
PDF is available here.
PDF is available here.
Abstract
Diagnosis of Strongyloides stercoralis is often delayed owing to patients presenting with nonspecific gastrointestinal complaints, a low parasite load and irregular larval output. Although several diagnostic methods exist to detect the presence of S. stercoralis there is no gold standard. In immunoc...
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PMID: 20733481
PDF is available here.
PDF is available here.
Acta Tropica (115)3 2010
Abstract
Recent studies suggest excretory-secretory (ES) antigen specific antibody detection tests to be of promising utility in laboratory diagnosis of many parasitic diseases in human including neurocysticercosis (NCC). The objective of the present study was to characterize the ES antigens collected from i...
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PMID: 20399738
PDF is available here.
PDF is available here.
Abstract
A retrospective study of the course and outcome of trichinellosis in a series of 50 patients hospitalized at the Institute for Infectious and Tropical Diseases in Belgrade between 2001 and 2008 was performed. Clinical diagnosis of trichinellosis was based upon the patients' clinical history, symptom...
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PMID: 21073142
PDF is available here.
PDF is available here.
Parasitology (137)10 2010
Abstract
No difference was found in microfilarial density between participants with and without epilepsy (P=0.498). The antibody index was raised in 2 participants. CSF PCR was negative in all samples tested. DISCUSSION: Our results do not give evidence of a relationship between O. volvulus and epilepsy. Des...
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PMID: 20388236
PDF is available here.
PDF is available here.
Infection and Immunity (78)9 2010
Abstract
We used green fluorescent protein/interleukin-4 (IL-4) reporter mice and truncated infection studies to identify both the tissue site and mechanism(s) by which the host protects itself from reinfection with N. brasiliensis. Strikingly, we demonstrated that the lung is an important site for priming i...
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PMID: 20605978
PDF is available here.
PDF is available here.
Infection and Immunity (78)9 2010
Abstract
Infection with the fish parasite Anisakis following exposure to contaminated fish can lead to allergic reactions in humans. The present study examined the immunological mechanisms underlying the development of allergic airway inflammation in mice after different routes of sensitization to Anisakis....
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PMID: 20605987
PDF is available here.
PDF is available here.
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (26)8 2010
Abstract
To investigate the immune responses and the protection induced by the transgenic Alfalfa (Medicago sativa) containing Eg95-EgA31 fusion gene of Echinococcus granulosus against Eg protoscoleces.
The leaf protein was extracted from the transgenic alfalfa by heat-coagulation method, its concentration w...
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PMID: 20619100
PDF is available here.
PDF is available here.
Parasitology Research (107)3 2010
Abstract
Enolase is a key enzyme in the glycolytic pathway; recent studies have also shown that enolase is found on the surface of several parasites, where it acts as a plasminogen-binding protein. In the present study, the enolase of Schistosoma japonicum has been cloned and expressed. In western blot analy...
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PMID: 20512506
PDF is available here.
PDF is available here.
Veterinary Parasitology (171)3-4 2010
Abstract
The objective of this randomised pairwise survey was to compare the regional distribution of antibody levels against the three most important helminth infections in organic and conventional dairy herds in Sweden. Bulk-tank milk from 105 organic farms and 105 neighbouring conventional dairy farms wit...
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PMID: 20466488
PDF is available here.
PDF is available here.
Abstract
Schistosoma mansoni ATP diphosphohydrolase isoforms and potato apyrase share conserved epitopes. By enzyme-linked immunosorbent assays, elevated levels of IgM, IgG2a and IgG1 antibody reactivity against potato apyrase were observed in S. mansoni-infected BALB/c mice during the acute phase of infecti...
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PMID: 20721478
PDF is available here.
PDF is available here.
Abstract
Our main focus has been on the plasma chemokines (CXCL8/CCL2/CCL3/CCL24) and chemokine receptors (CCR2/CCR3/CCR5/CXCR1/CXCR2/CXCR3/CXCR4) expressed by circulating eosinophils from acute Schistosoma mansoni infected patients (ACT). Our studies compared ACT patients and healthy individuals as a contro...
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PMID: 20721479
PDF is available here.
PDF is available here.
Abstract
We showed for the first time that the conserved domains within Schistosoma mansoni ATP diphosphohydrolase isoforms, shared with potato apyrase, possess epitopes for the IgG1 and IgG4 subtypes, as 24 (80%) of the 30 schistosomiasis patients were seropositive for this vegetable protein. The analyses f...
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PMID: 20721477
PDF is available here.
PDF is available here.
Abstract
This paper deals with current knowledge of the interrelationships between Schistosoma infection and malnutrition. It emphasizes the relevance of these investigations in the face of dynamic and evolving changes occurring in population diets and changes in the epidemiological patterns of schistosomias...
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PMID: 20721475
PDF is available here.
PDF is available here.
Infection and Immunity (78)7 2010
Abstract
We investigated the effects of chronic infections with Ascaris lumbricoides and Trichuris trichiura (measured twice over a 5-year period) on cytokine and antibody responses. We collected blood from 1,060 children aged 4 to 11 years living in a poor urban area of Brazil and measured Th1 (gamma interf...
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PMID: 20404082
PDF is available here.
PDF is available here.
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases (28)3 2010
Abstract
To study the protective immunity induced by recombinant vaccination of Cs-Rho GTPase of Clonorchis sinensis (Cs).
20 SD-rats(8 weeks) were divided into two groups: A (recombinant protein experiment group) and B (PBS control group). Rats in group A were immunized with 1 ml protein of Cs-Rho GTPase (9...
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PMID: 20806498
PDF is available here.
PDF is available here.
Veterinary Record (166)24 2010
Abstract
Abstract
We therefore investigated the anti-Toxocara canis antibody profile in the aqueous humor in an animal model of ocular toxocariasis. We intravitreally injected T. canis larvae into the right eye of 4 rabbits; 2 rabbits were orally administered T. canis eggs. We collected serum, aqueous humor, and tear...
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PMID: 19963075
PDF is available here.
PDF is available here.
Abstract
Human pulmonary and subcutaneous dirofilariosis caused by Dirofilaria immitis and Dirofilaria repens are worldwide diagnosed with increasing frequency. These species are responsible for the development of benign pulmonary and subcutaneous nodules, respectively, that can be confused with lung or cuta...
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PMID: 20197111
PDF is available here.
PDF is available here.
Journal of Parasitology (96)3 2010
Abstract
We evaluated whether the doxycholic acid extract of Strongyloides venezuelensis stage 3 larvae was able to protect CD1 mice against a homologue infection. Moreover, we included saponins from Quillaja saponaria (Qs) and immunomodulatory substances, i.e., Phlebodium pseudoaureum hydroalcoholic extract...
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PMID: 20557210
PDF is available here.
PDF is available here.
Abstract
After recreational exposure to river water in Uganda, 12 (17%) of 69 persons had evidence of schistosome infection. Eighteen percent self-medicated with praziquantel prophylaxis immediately after exposure, which was not appropriate. Travelers to schistosomiasis-endemic areas should consult a travel...
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PMID: 20409387
PDF is available here.
PDF is available here.
Abstract
Immunization with Schistosoma or Fasciola worm homogenates, mixed either with or without saponin, recorded an amelioration of the free radical scavenger levels, liver function enzymes and reduction in worm burden, as well as improvement of the histological feature of the liver, the number and size o...
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PMID: 20539066
PDF is available here.
PDF is available here.
Abstract
Trichuris muris is a laboratory model for the human whipworm Trichuris trichiura which infects approximately 1 billion people in tropical and sub-tropical countries. The development of a vaccine would control trichuriasis by promoting the acquisition of immunity during childhood, thereby reducing fa...
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PMID: 19968992
PDF is available here.
PDF is available here.
Abstract
We investigated the value of an anti-Strongyloides IgG enzyme immunoassay (EIA), using a panel of 207 sera retrospectively collected from patients with definitive diagnoses of strongyloidiasis (n=57), other helminthic infections (n=46), eosinophilia without parasitic infection diagnosis (n=54), and...
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PMID: 20335415
PDF is available here.
PDF is available here.
Abstract
This study was designed to investigate the factors associated with the high rate of false-positive test results observed with the 4th-generation Murex HIV Ag/Ab Combination EIA (enzyme immunoassay) within an adolescent and young-adult cohort in northwest Tanzania. (4th-generation assays by definitio...
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PMID: 20181896
PDF is available here.
PDF is available here.
Veterinary Parasitology (169)1-2 2010
Abstract
We developed a SYBR Green-based, real-time RT-PCR protocol to quantitate cytokine mRNA in freshly harvested rabbit peripheral mononuclear cells. The method was validated in the course of a vaccination trial in which animals vaccinated with the recombinant antigen FhSAP2 were challenged with Fasciola...
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PMID: 20056331
PDF is available here.
PDF is available here.
Veterinary Parasitology (169)1-2 2010
Abstract
The impact of various milk handling stressors were analyzed using a commercially available enzyme-linked immunosorbent assay (ELISA) test measuring Ostertagia ostertagi antibodies in milk from dairy cattle (Svanovir). An indirect ELISA has the ability to determine the amount of milk production losse...
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PMID: 20053502
PDF is available here.
PDF is available here.
Veterinary Parasitology (169)1-2 2010
Abstract
We have developed a reliable diagnostic test for use in live animals that would enable veterinary regulators to focus disease control strategies. The test detects bovine anti-T. saginata immunoglobulin G1 antibodies using an enzyme-linked immunosorbent assay (ELISA) which relies on the excretory-sec...
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PMID: 20083357
PDF is available here.
PDF is available here.
Abstract
We tested sera from 495 allergic and 25 non-food-related allergic patients. The decay in specific IgE antibodies in serum was also investigated in 15 positive patients over a period of 6 to 38 months. Considering sera that tested positive by either Ani s 1 or Ani s 7 ELISA, the CAP FEIA classified 2...
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PMID: 20107002
PDF is available here.
PDF is available here.
Abstract
We developed and evaluated two magnetic immunochromatographic tests (MICTs) for detection of human Taenia solium taeniasis antibodies (ES33-MICT) and neurocysticercosis antibodies (T24-MICT). These assays detected stage-specific antibodies by using two recombinant proteins, rES33 for detection of ta...
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PMID: 20181766
PDF is available here.
PDF is available here.
Abstract
Taenia saginata metacestode antigens have been constituted a useful alternative antigen for neurocysticercosis (NC) serodiagnosis, particularly due to an increasing difficulty to obtain Taenia solium homologous antigen. Cross-reactivity with Echinococcus granulosus infection occurs i...
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PMID: 20130125
PDF is available here.
PDF is available here.
Abstract
Human neurocysticercosis (NC) is endemic in most countries of Latin America, Asia and Africa and is re-emerging in some industrialized nations. Both within and among endemic countries, NC is very variable in its clinical and radiological features, as well as in the intensity of the immuno-inflammato...
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PMID: 20116079
PDF is available here.
PDF is available here.
Serological, clinical and epidemiological evaluation of toxocariasis in urban areas of south Brazil.
Abstract
Toxocariasis is a worldwide public-health problem that poses major risks to children who may accidentally ingest embryonated eggs of Toxocara. The objectives of this study were to investigate the occurrence of anti-Toxocara spp. antibodies in children and adolescents and the variables that may be in...
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PMID: 20464126
PDF is available here.
PDF is available here.
Parasitology (137)4 2010
Abstract
Microfilariae were detected in 14 cases (11.8%), whereas the Og4C3 assay could detect filarial antigen in 44.5% of pregnant mothers. Interestingly, 24.5% of samples born from CFA-positive mothers were found positive for CFA. None of the cord samples from CFA-negative mothers were found positive for...
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PMID: 19849889
PDF is available here.
PDF is available here.
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases (28)2 2010
Abstract
DNA sequencing confirmed that the nucleotide sequence of synthesized IPSE gene was completely identical to the native one. SDS-PAGE showed that the recombinant plasmid IPSE/pET32a(+) expressed a fusion protein with an Mr 35700 after being induced by IPTG. The pure fusion protein Trx-IPSE reacted pos...
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PMID: 20666307
PDF is available here.
PDF is available here.
Neurological Research (32)3 2010
Abstract
Since the late 1980s, several clinical investigations have led to a better understanding of the natural history of this disorder and an improved characterization of its clinical presentations. Spinal cord schistosomiasis is a severe condition that typically presents as an acute/subacute low cord syn...
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PMID: 20406603
PDF is available here.
PDF is available here.
Abstract
All patients had bathed in the waters of a regional river, the Araguaia. Six of them presented with intermediate uveitis, with snowbanking. Five had cataract and four showed inferior endothelial opacity, with localized anterior synechiae. One showed total leukoma, with flat anterior chamber. Only tw...
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PMID: 19165423
PDF is available here.
PDF is available here.
Abstract
Human intestinal capillariasis caused by Capillaria philippinensis is characterized by chronic diarrhea which may lead to death if left untreated. The mortality is highest among patients who are negative by conventional stool examination. Therefore this study explored the application of an enzyme-li...
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PMID: 20578511
PDF is available here.
PDF is available here.
Epidemiology and Infection (138)3 2010
Abstract
Dirofilaria immitis is the causal agent of canine and feline cardiopulmonary dirofilariasis. Moreover, the existence of canine dirofilariasis implies a risk for human populations living in an endemic area in which, the parasite can cause pulmonary dirofilariasis. The Spanish epidemiological situatio...
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PMID: 19619388
PDF is available here.
PDF is available here.
Abstract
The aim of this study was to assess the seroprevalence of human toxocariasis in three Andean communities from the Northeast of Lima, Peru. A total of 303 subjects including children and adults were studied and blood samples were collected to detect anti-Toxocara antibodies by ELISA-IgG test and by h...
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PMID: 20305952
PDF is available here.
PDF is available here.
Abstract
The aim of this study was to determine the seroprevalence of the infection by Toxocara in the general population of the Amazonian city of Yurimaguas, Peru. From March to August 2008, a total of 300 subjects were sampled and tested by means of a Toxocara ELISA-IgG test. A clinical and epidemiological...
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PMID: 20305953
PDF is available here.
PDF is available here.
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases (28)1 2010
Abstract
The sensitivity for detecting schistosomiasis antibodies with DLIA and ELISA was 94.2% (65/69) and 95.7% (66/69), respectively (chi2=0.15, P>0.05). The specificity in examining healthy persons was 97.4% (257/264) and 94.7% (250/264), respectively (chi2=2.43, P>0.05). No cross reaction was found with...
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PMID: 20411746
PDF is available here.
PDF is available here.
Abstract
We carried out a community-based cross-sectional study in 454 individuals by examining at least two stool samples with different methods for soil-transmitted helminths (Ascaris lumbricoides, hookworm, Strongyloides stercoralis, and Trichuris trichiura) and one urine sample for Schistosoma haematobiu...
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PMID: 20485491
PDF is available here.
PDF is available here.
Abstract
Mice responses to immunization with Schistosoma mansoni antigens were investigated. Priming with cercarial antigen preparation (CAP) induced significant (P < 0.05) IgM, IgG, IgG2a, IgG2b, and IgA increases, while booster caused a significant IgG1 increase. A soluble worm antigen preparation (SWAP) c...
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PMID: 20469651
PDF is available here.
PDF is available here.
Abstract
Taenia solium is an important zoonosis in many developing countries. Cysticercosis poses a serious public health risk and incurs sizeable economic losses to pig production. Because data on the epidemiology of porcine cysticercosis in Mozambique are scarce, the present study was conducted to determin...
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PMID: 20126403
PDF is available here.
PDF is available here.
Clinical laboratory (56)11-12 2010
Abstract
The detection of antibodies in saliva samples proved to be effective in the diagnosis of several microbial diseases. These antibodies were screened in saliva samples of patients with hydatid cysts.
Anti-hydatid fluid antigen IgG and IgA antibodies were screened in saliva and sera of patients with hy...
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PMID: 21141438
PDF is available here.
PDF is available here.
Parasitology (137)1 2010
Abstract
Scarce and inconclusive information on general biological impact of Toxocara invasion on paratenic hosts, and people in particular, has led us to undertake a comprehensive study of the problem. The study has been conducted in a rural environment, which is considered a toxocarosis risk factor. In tot...
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PMID: 19691865
PDF is available here.
PDF is available here.
Abstract
Khirurgiia ()1 2010
Abstract
Results of the immune-enzyme assay (IEA) of children with liver and lung echinococcosis before and after the operation were analyzed. The IEA accuracy was 91% for liver echinococcosis and 57% for lung echinococcosis. Sensitivity was 86% and 55, respectively. IEA was a reliable method of disease recu...
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PMID: 20336041
PDF is available here.
PDF is available here.
Abstract
The diagnostic effectiveness of instrumental analysis of the concentration of specific antibodies, which was expressed in antibody units and titers, was compared in the test system of enzyme immunoassay using Echinococcus granulosus on 76 serum samples from patients with echinococcosis. The factors...
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PMID: 20361629
PDF is available here.
PDF is available here.
MMWR. Surveillance summaries : Morbidity and mortality weekly report. Surveillance summaries / CDC (58)9 2009
Abstract
During 2002-2007, a total of 66 cases of trichinellosis were reported to CDC. Those cases for which a supplementary case report form was not submitted or that did not meet the case definition were excluded from analysis. Of the 66 cases reported during 2002-2007, a total of 12 (18%) cases were exclu...
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PMID: 19959986
PDF is available here.
PDF is available here.
Abstract
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases (27)6 2009
Abstract
Tetraspanin 2-A (SjTsp2-A) gene was amplified by PCR. pcDNA3.1(+)/SjTsp2-A recombinant plasmids were constructed and transformed into E. coli DH5alpha. Twenty four BALB/c mice were randomly divided into pcDNA3.1(+)/ -SjTsp2-A group (A), pcDNA3.1(+)/SjGST group (B) and pcDNA3.1(+) group (C). Each mou...
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PMID: 20232641
PDF is available here.
PDF is available here.
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases (27)6 2009
Abstract
Three towns with reported echinococcosis cases and five randomly selected towns were chosen and Echinococcus infection in pupils (7-12 years old) was investigated serologically in 2008. The sero-positive rate (IgG) was 0.9% (25/2 768). There was no significant statistical difference on the IgG posit...
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PMID: 20232642
PDF is available here.
PDF is available here.
Abstract
Specific IgG (P<0.0001), IgE (P<0.05), IL-4 (P=0.0197) and IL-10 (P<0.01) levels were significantly elevated in CE cases compared to healthy controls. IL-4 could be detected in 34 patients (75.55%) and six controls (60%) in a low concentration. The IgE concentration was elevated (>120 U/ml) in 36 (8...
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PMID: 20090135
PDF is available here.
PDF is available here.
Journal of Helminthology (83)4 2009
Abstract
Exposure of encysted metacercariae of Clinostomum complanatum to UV light (254 nm) for 60 min reduced their development into adult worms in buff-backed herons (95.7% reduction in worm burden). Metacercariae that succeeded in developing into adult worms, showed low fecundity levels. Furthermore, 30%...
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PMID: 19435543
PDF is available here.
PDF is available here.
Journal of Helminthology (83)4 2009
Abstract
An immunobinding dot-blot assay (DBA) was developed on nitrocellulose paper for the serodiagnosis of human cysticercosis, using Cysticercus cellulosae as antigen. The DBA had an immunological sensitivity of 0.08 mg of antigen protein/ml; however, it showed cross-reactions with antigens of adult Taen...
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PMID: 19317923
PDF is available here.
PDF is available here.
Journal of Helminthology (83)4 2009
Abstract
The prevalence of Brugia malayi was surveyed in three highly endemic provinces in southern Thailand as part of an ongoing evaluation of the effectiveness of two rounds of mass drug administration (MDA). Prior to MDA IV, and thereafter every 6 months up to 18 months, including MDA V at 12 months, noc...
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PMID: 19422729
PDF is available here.
PDF is available here.