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Antibodies, Immobilized (0):
Recent article
Antibodies, Immobilized
Abstract
I monoclonal antibodies (Mabs) were prepared by hybridoma technique and firstly used to develop a Sudan I immunosensor by immobilizing the Mabs on a gold electrode. o-Mercaptobenzoic acid (MBA) was covalently conjugated on the gold electrode to form a self-assembled monolayer (SAM). The immobilizati...
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PMID: 21315921
PDF is available here.
PDF is available here.
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Abstract
Antibody-based separation methods, such as immunoaffinity chromatography (IAC), are powerful purification and isolation techniques. Antibodies isolated using these techniques have proven highly efficient in applications ranging from clinical diagnostics to environmental monitoring. IAC is an efficie...
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PMID: 20978959
PDF is available here.
PDF is available here.
Abstract
We examined the kinetics of ERK1/2 phosphorylation and TNF-α secretion in primary human monocytes in response to soluble heat-aggregated IgG or surface-bound IgG, to mimic soluble immune complexes and tissue-deposited IgG, respectively. Soluble aggregated IgG induced transient signaling, leading to...
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PMID: 20937257
PDF is available here.
PDF is available here.
Abstract
This paper reports on an inkjet printing method for the fabrication of lateral flow immunochromatographic devices made from a single piece of filter paper by patterning microfluidic channels and dispensing immunosensing inks, requiring only a single printing apparatus. This "paperfluidic" immunosens...
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PMID: 20652543
PDF is available here.
PDF is available here.
Abstract
We demonstrate that the biosensor was able to detect SEB at concentrations as low as 5 ng/mL at a signal to baseline (S/B) ratio of 2. Such measurements were performed on the chip in wet conditions. The actuation of the chip by SEB is immediate, permitting real-time signal measurements. In addition...
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PMID: 20447819
PDF is available here.
PDF is available here.
Abstract
A simple biosensing strategy for the diagnosis of patients with hepatitis B virus (HBV) was developed. This study can be divided into two themes, both of which utilized gold-binding polypeptide (GBP) fusion proteins: HBV surface antigen PreS2 (HBsAg) detection with GBP-fused single chain antibody (G...
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PMID: 20602973
PDF is available here.
PDF is available here.
Abstract
We prepared a polymer in which the Cu(II) was embedded. The Cu(II)-embedded polymer was tested for its binding with protein. It was found that Cu(II)-embedded polymer displayed a strong binding with bovine serum albumin (BSA). At 80% of methanol, no BSA was released from Cu(II)-embedded polymer. The...
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PMID: 20602958
PDF is available here.
PDF is available here.
Abstract
We have synthesized a biocompatible polyaspartic acid-based polymer (molecular weight approximately 15,000-25,000) with cysteine on its backbone for use as a capping ligand for functionalized Au, Ag, and CdSe@ZnS nanoparticles. Nearly monodisperse, hydrophobic Au and Ag nanoparticles and CdSe@ZnS qu...
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PMID: 20112990
PDF is available here.
PDF is available here.
Abstract
A novel electrochemical immunosensor for sensitive detection of cancer biomarker alpha-fetoprotein (AFP) is described that uses a graphene sheet sensor platform and functionalized carbon nanospheres (CNSs) labeled with horseradish peroxidase-secondary antibodies (HRP-Ab2). Greatly enhanced sensitivi...
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PMID: 20201502
PDF is available here.
PDF is available here.
Abstract
Analysis of panel of antibodies to HBsAg produced by 6 hybridomas revealed pH-dependent monoclonal antibodies (18C8), which immobilization at low pH values together with detecting antibodies F4F3 allowed to greatly improve sensitivity of the sandwich technique. Minimal credibly detectable concentrat...
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PMID: 20468098
PDF is available here.
PDF is available here.
Abstract
Surface plasmon resonance (SPR) is a highly sensitive method for the detection of molecular interactions. One interacting partner is immobilized on the sensor chip surface while the other is injected across the sensor surface. This chapter focuses on high-affinity immobilization of protein substrate...
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PMID: 20217614
PDF is available here.
PDF is available here.
Abstract
Rapid immunochromatographic test for dengue diagnosis shows good reproducibility and specificity, with an acceptable sensitivity. Therefore, this rapid test is recommended as diagnostic tool in endemic areas....
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PMID: 20440460
PDF is available here.
PDF is available here.
Abstract
We introduce an automated immunoblotting method that reports protein electrophoretic mobility and identity in a single streamlined microfluidic assay. Native polyacrylamide gel electrophoresis (PAGE) was integrated with subsequent in situ immunoblotting. Integration of three PA gel elements into a g...
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PMID: 19731927
PDF is available here.
PDF is available here.
Abstract
We employed microfabricated surfaces to capture T-cells from minimally processed human blood, arrange these cells into a single cell array, and then detect interferon (IFN)-gamma released from individual cells. The fabrication of cell capture surfaces started with coating a silane-modified glass sli...
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PMID: 19739655
PDF is available here.
PDF is available here.
Abstract
We report the first electrochemiluminescent immunosensor combining single-wall carbon nanotube forests with RuBPY-silica-secondary antibody nanoparticles for sensitive detection of cancer biomarker prostate specific antigen.
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PMID: 19668818
PDF is available here.
PDF is available here.
Abstract
Continuous outbreaks of avian influenza (AI) in recent years with increasing threat to animals and human health have warranted the urgent need for rapid detection of pathogenic AI viruses. In this study, an impedance immunosensor based on an interdigitated array (IDA) microelectrode was developed as...
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PMID: 19559858
PDF is available here.
PDF is available here.
Abstract
We developed a sensitive method to detect Escherichia coli O157:H7 based on enhancement with immuno-nanoparticles. METHOD: About 10 nm ultrafine immunomagnetic particles were prepared by a co-precipitation method, about 20 nm immuno-colloid-gold was prepared by sodium citrate reduction. The immunose...
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PMID: 19673420
PDF is available here.
PDF is available here.
Abstract
The CBIB was capable of distinguishing among the three analytes. The sensitivity and dynamic ranges of the assay were suitable for the detection of the three targets in the majority of GCF samples. There were highly statistically significant (P <0.0001) positive correlations between CBIB and ELISA d...
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PMID: 19254129
PDF is available here.
PDF is available here.
Methods in Molecular Biology (527)527 2009
Abstract
In view of the importance of information transfer mediated throughout the cell by recognition, phosphorylation or dephosphorylation of kinases, their adapters, or substrates, this method was developed. The method provides a potent research tool for rapidly generating and testing these substrates as...
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PMID: 19241019
PDF is available here.
PDF is available here.
Methods in Molecular Biology (527)527 2009
Abstract
We have described in detail two methods for differential analysis of protein tyrosine phosphorylation in cells by (1) using a single fluorescent protein capture format on membrane array and (2) a competitive protein capture method on glass surface array....
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PMID: 19241018
PDF is available here.
PDF is available here.
Abstract
Our results demonstrate that the immunoaffinity column with immobilized BBA-2187 scFv antibody is efficient for the cleanup of BPA-contaminated water samples from different sources....
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PMID: 19102649
PDF is available here.
PDF is available here.
Abstract
The results indicate that the methods compared are all adequate for diagnosing Legionella infection, although some have certain limitations regarding sensitivity....
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PMID: 19254639
PDF is available here.
PDF is available here.
Analytical Biochemistry (385)1 2009
Abstract
We previously reported a silica-binding protein, designated Si-tag, which can be used to immobilize proteins on silica surfaces. Here, we constructed a fusion protein of Si-tag and immunoglobulin-binding staphylococcal protein A for oriented immobilization of antibodies on a silicon wafer whose surf...
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PMID: 19017523
PDF is available here.
PDF is available here.
Abstract
We have developed a dry-reagent dipstick test for simultaneous visual detection of two alleles in single nucleotide polymorphisms (SNPs). The strip comprises two test zones and a control zone. Oligonucleotide-functionalized gold nanoparticles are used as reporters. PCR-amplified DNA that spans the i...
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PMID: 18685560
PDF is available here.
PDF is available here.
Journal of Molecular Biology (384)5 2008
Abstract
We describe the generation, affinity maturation, and characterization of a fully human monoclonal antibody to human NKG2D. Using phage display technology based on a newly generated naïve human Fab library in phage display vector pC3C followed by a tandem chain shuffling process designed for minimal...
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PMID: 18809410
PDF is available here.
PDF is available here.
Abstract
We established a technique for constructing PEGylated gold nanoparticles (GNP) that have small compounds with almost complete functionalities on their surfaces using a newly synthesized hetero-telechelic poly(ethylene glycol) (PEG), and their association/dissociation behavior on an antibody-immobili...
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PMID: 18985213
PDF is available here.
PDF is available here.
Abstract
Restricted access media using antibodies as immobilized ligands were developed for the rapid and selective capture of small analytes by immunoextraction, giving rise to materials referred to as immunoaffinity restricted access media (IA-RAM). To make such a material, intact antibodies for the desire...
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PMID: 18937423
PDF is available here.
PDF is available here.
Abstract
We describe a simple empirical method to correct the apparent/observed affinity to account for the blinking contribution. The combination of many single molecule affinity measurements from different antibodies yields not only the average affinity, it directly measures the full shape and character of...
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PMID: 18847284
PDF is available here.
PDF is available here.
Journal of Chromatography B (875)2 2008
Abstract
We developed a porous silicon (PSi) affinity chip to detect BNP present at low concentrations in human plasma by matrix assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS) directly. The PSi surface immobilized with antibodies captured and concentrated BNP through ant...
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PMID: 18948066
PDF is available here.
PDF is available here.
Abstract
Analytical Biochemistry (296)1 2001
Abstract
We developed an enantioselective immunoaffinity extraction method, which employs an immobilized antibody to specifically isolate peptide fragments that have been modified with optically active ibuprofen. Rabbits were immunized with (S)-ibuprofen coupled to bovine serum albumin through a beta-alanine...
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PMID: 11520033
PDF is available here.
PDF is available here.