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Antibodies, Protozoan (0):
Recent article
Antibodies, Protozoan
Abstract
These results indicate that the PATH-Lemos rapid test shows promise as an improved and reliable tool for screening and diagnosis of Chagas disease....
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PMID: 21342808
PDF is available here.
PDF is available here.
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Abstract
Frequency and levels of IgG antibodies to an N-terminal fragment of the Plasmodium vivax MSP-1 (Pv200L) protein, in individuals naturally exposed to malaria in four endemic areas of Brazil, were evaluated by enzyme-linked immunosorbent assay. Plasma samples of 261 P. vivax-infected i...
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PMID: 21292879
PDF is available here.
PDF is available here.
Abstract
A 1-year-old 32.5-kg (71.5-lb) sexually intact male foxhound-Treeing Walker Coonhound cross was evaluated because of a 2.5-month history of dermatologic lesions, weight loss, and diarrhea.
Physical examination revealed muscle wasting, lymphadenopathy, and multifocal pruritic dermatologic lesions of...
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PMID: 21118014
PDF is available here.
PDF is available here.
Abstract
We used the direct agglutination test (DAT) and the rK39 ELISA as L. donovani infection markers in 10 VL endemic villages in Nepal. DAT titre distribution showed two subgroups in the population (infected and non-infected individuals), while rK39 did not. The agreement between both tests was moderate...
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PMID: 21998875
PDF is available here.
PDF is available here.
Abstract
These results indicate that N. caninum may provide a new tool for the production of a live recombinant vector vaccine against toxoplasmosis in animals. To our knowledge, this is the first report to evaluate the usefulness of N. caninum-based live vaccine.
Copyright © 2010 Elsevier Ltd. All rights r...
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PMID: 20832493
PDF is available here.
PDF is available here.
Abstract
We constructed an Ad-based malaria vaccine by inserting a B cell epitope derived from a Plasmodium yoelii circumsporozoite (CS) protein (referred to as the PyCS-B epitope) into the capsid proteins of WT/CS-GFP, a recombinant Ad expressing P. yoelii CS protein and GFP as its transgene. Multiple vacci...
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PMID: 20811151
PDF is available here.
PDF is available here.
Abstract
We examine prevalence of antibodies to Toxoplasma gondii, and four morbilliviruses (canine distemper [CDV], phocine distemper [PDV], dolphin morbillivirus [DMV], porpoise morbillivirus [PMV]) including risk factors for exposure. We also examine the relationships between antibody levels and hematolog...
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PMID: 20607348
PDF is available here.
PDF is available here.
Abstract
The Chagas disease particle gel immunoassay (PaGIA-Chagas) is a simple, fast and practical test used for the diagnosis of the chronic Chagas disease and is based on anti-Trypanosoma cruzi antibody detection in serum. This study aimed to validate the PaGIA-Chagas on plasma collected with capillary tu...
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PMID: 21045375
PDF is available here.
PDF is available here.
Abstract
In this study, serum samples of 203 animals from different locations, from zoos and breeding facilities from the north and northeast regions of Brazil, were analyzed for the presence of anti-Toxoplasma gondii antibodies by the modified agglutination test (MAT) with a cutoff of 1:25. Of the sampled a...
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PMID: 20945665
PDF is available here.
PDF is available here.
Abstract
Serum samples from 2970 (1400 sows, 1570 fattening) pigs, from 100 farms in the 10 main swine production regions in Spain were tested for antibodies against T. gondii by the modified agglutination test (MAT). Antibodies to T. gondii (MAT 1:25 or higher) were detected in 492 pigs (16.6%, 9.7% in fatt...
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PMID: 20601110
PDF is available here.
PDF is available here.
Abstract
We collected 210 human serum samples and tested them by the dye test using in vivo tachyzoites (conventional method) then compared these results with three other methods: a dye test using cell culture-derived T. gondii tachyzoites and indirect immunofluorescent antibody tests (IFAT) using in vivo an...
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PMID: 21073025
PDF is available here.
PDF is available here.
Veterinary Parasitology (172)1-2 2010
Abstract
These results suggested that C-Cp12-Cp21-DNA may provide an effective means of eliciting humoral and cellular responses and generating protective immunity against C. parvum infections in BALB/c mice.
(c) 2010 Elsevier B.V. All rights reserved....
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PMID: 20541869
PDF is available here.
PDF is available here.
Parasitology Research (107)3 2010
Abstract
We describe the possible use of the clone-9 protein for this purpose, which was identified as a potential immunogenic piroplasm protein by random sequencing of cDNA library clones followed by bioinformatic analyses. The clone-9 gene was partially recombinantly expressed and used for the development...
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PMID: 20422216
PDF is available here.
PDF is available here.
Epidemiology and Infection (138)8 2010
Abstract
The aim of this study was to analyse the spatio-temporal dynamics of Toxoplasma gondii infection in long-term monitoring of domestic cats (8-15 years) in three populations living in rural France. Overall seroprevalence was 52.7% (modified agglutination test > or =1:40). Incidence was 0.26-0.39 seroc...
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PMID: 19961642
PDF is available here.
PDF is available here.
Infection and Immunity (78)8 2010
Abstract
We infected BALB/c PKC-theta(-/-) and PKC-theta(+/+) wild-type mice with Toxoplasma gondii. The PKC-theta(-/-) mice succumbed to necrotizing Toxoplasma encephalitis due to an insufficient parasite control up to day 40, whereas the wild-type mice survived. The number of T. gondii-specific CD4 and CD8...
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PMID: 20498263
PDF is available here.
PDF is available here.
Abstract
We quantified prevalence of antibody to T. gondii in two threatened avian marine species, Galapagos Penguins (Spheniscus mendiculus) and Flightless Cormorants (Phalacrocorax harrisi), and tested the hypothesis that this parasite is more prevalent on Isabela Island (with cats) than on Fernandina Isla...
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PMID: 20688714
PDF is available here.
PDF is available here.
Abstract
We investigated whether prevalence of antibodies to Toxoplasma gondii in woodchucks (Marmota monax) was related to urbanization in a Midwestern landscape. We collected serum samples from adult woodchucks captured across an urban-rural gradient in Illinois, USA in May-November 2007. We used an indire...
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PMID: 20688709
PDF is available here.
PDF is available here.
Parasitology Research (107)2 2010
Abstract
We reported a membrane-bound PTPase activity in promastigotes of Leishmania major. In the present work, we give evidence that promastigotes of L. mexicana are able to secrete a PTPase into the culture medium. Two antibodies: one monoclonal against the catalytic domains of the human placental PTPase...
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PMID: 20405143
PDF is available here.
PDF is available here.
Parasitology Research (107)2 2010
Abstract
We have investigated the expression level of surface cruzipain in 16 field isolates belonging to the genotype TCI (n = 8) and TCII (n = 8) of T. cruzi. By means of flow cytometry analyses, using an anti-cruzipain polyclonal antibody, we observed a highly heterogeneous pattern of surface cruzipain mo...
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PMID: 20512588
PDF is available here.
PDF is available here.
Parasitology Research (107)2 2010
Abstract
A cross-sectional study was conducted to determine the occurrence of anti-Toxoplasma gondii, anti-Neospora caninum, and anti- Leishmania chagasi antibodies in dogs of the state of Pará, Brazil. For this purpose, 129 blood samples were collected from dogs of different ages and gender. Samples of 72...
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PMID: 20445991
PDF is available here.
PDF is available here.
Veterinary Record (166)26 2010
Abstract
An indirect ELISA based on a soluble extract of Besnoitia besnoiti tachyzoites was developed and standardised. A set of positive and negative reference bovine sera were characterised using an immunofluorescence antibody test and Western blot. A cut-off with a relative index per cent of 8.1 was deter...
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PMID: 20581359
PDF is available here.
PDF is available here.
Abstract
We synthesized a DIF derivative (DIF-1-NH(2); 6-amino-1-(3,5-dichloro-2,6-dihydroxy-4-methoxyphenyl)hexan-1-one), and prepared an anti-DIF-1 antibody using a DIF-1-NH(2)-conjugated macromolecule as the immunogen. A 100-fold dilution of the antibody bound to DIF-1-NH(2)-conjugated resin, and this bin...
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PMID: 20416278
PDF is available here.
PDF is available here.
Abstract
Toxoplasmosis is an infection caused by Toxoplasma gondii, an intracellular obligate parasite. Its transmission has usually been attributed to ingestion of undercooked or raw meat. The frequency of T. gondii in pork, the most common meat for human consumption in Jalisco, Mexico, is unknown; in Guada...
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PMID: 20537271
PDF is available here.
PDF is available here.
Abstract
A total of 179 pregnant women were studied. Almost 50.8% were between the ages of 25-30 years, 70.8% of the patients studied, had tertiary education. An assessment of the patients' status to anti-toxoplasmosis IgG showed 40.8% were positive while 59.2% were negative. Pet-keeping was a practice among...
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PMID: 20539334
PDF is available here.
PDF is available here.
Abstract
Leishmania (Viannia) shawi was recently characterized and few studies concerning modifications in cellular and humoral immune responses in experimental leishmaniasis have been conducted. In this work, immunopathological changes induced by L. shawi in chronically infected BALB/c mice were investigate...
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PMID: 20060062
PDF is available here.
PDF is available here.
Abstract
Glutathione S-transferase (FhGST) purified from Fasciola hepatica adult worms was used to immunise goats against F. hepatica in an experimental infection; the level of protection, in terms of fluke burden, faecal egg counts and hepatic damage was determined, as well as the humoral and cellular immun...
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PMID: 20035898
PDF is available here.
PDF is available here.
Abstract
A gene coding for a protein with sequence similarity to the Toxoplasma gondii micronemal 1 (MIC1) protein that contains a copy of a domain described as a sialic acid-binding micronemal adhesive repeat (MAR) was identified in the Babesia bovis genome. The single copy gene, located in chromosome 3, co...
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PMID: 20304092
PDF is available here.
PDF is available here.
Journal of Parasitology (96)3 2010
Abstract
The seroprevalence of toxoplasmosis in pigs was examined in China's southern Guangdong Province from March 2008 through May 2009. A total of 1,022 serum samples was collected from 12 administrative cities and assayed for Toxoplasma gondii antibodies by enzyme-linked immunosorbent assay test. The ser...
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PMID: 20557218
PDF is available here.
PDF is available here.
Journal of Parasitology (96)3 2010
Abstract
To investigate how different routes of Toxoplasma gondii transmission influence the antibody response and infection status of deer mice (Peromyscus maniculatus), 80 mice were orally infected with 1, 5, 10, or 100 T. gondii oocysts. Ten weeks postinfection, 15 T. gondii -seropositive female mice were...
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PMID: 20557196
PDF is available here.
PDF is available here.
Journal of Parasitology (96)3 2010
Abstract
We sought to determine the prevalence of T. gondii infection and associated epidemiological characteristics in 472 patients in Durango, Mexico. Participants were tested for T. gondii IgG and IgM antibodies. In addition, sociodemographic, clinical, and behavioral characteristics from each participant...
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PMID: 20557194
PDF is available here.
PDF is available here.
Journal of Parasitology (96)3 2010
Abstract
Our data strongly suggest the application of recently isolated parasites as sources of antigen for diagnosis procedures. Moreover, L. naiffi species possesses several characteristics relevant for its use as a source of novel antigens to be explored in the design of diagnostic tools and vaccines....
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PMID: 20557195
PDF is available here.
PDF is available here.
Journal of Parasitology (96)3 2010
Abstract
We report a seroprevalence of 6.4% among 860 canines from 31 counties and 5 ecoregions throughout Tennessee, using an indirect immunofluorescent assay (IFA). Statistically significant associations between seropositivity and age, weight, and outdoor living were noted. Differences in seropositivity we...
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PMID: 20557201
PDF is available here.
PDF is available here.
Folia Parasitologica (57)2 2010
Abstract
We assessed IgG antibody to Toxoplasma gondii in 300 inpatients with schizophrenia (SG), 150 outpatients with anxiety and depressive disorders (PCG), and 150 healthy blood donors (HCG). Seropositivity rates were 60.7% for SG, 36.7% for PCG, and 45.3% for HCG (p<0.001). The seropositivity rate for an...
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PMID: 20608474
PDF is available here.
PDF is available here.
Neuroscience Letters (475)3 2010
Abstract
We aimed to investigate the possible association between Toxoplasma infection and PD by evaluating the serum anti-Toxoplasma gondii IgG antibodies. There were no difference between the socioeconomic status of the patients and control subjects and magnetic resonance images of the patients were normal...
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PMID: 20350582
PDF is available here.
PDF is available here.
Veterinary Record (166)19 2010
Abstract
Abstract
In malaria, drug resistance and treatment failure (TF) are not synonymous, although are escalating together. Over 9 years of surveillances for malaria morbidity and TF in Daraweesh village in eastern Sudan (1991-2004), 136 donors (15-78 years) from 43 households, treated for 278 mala...
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PMID: 20215002
PDF is available here.
PDF is available here.
Microbes and Infection (12)5 2010
Abstract
Trypanosoma cruzi-specific immune responses were evaluated in a total of 88 subjects living in areas endemic of Chagas disease of Argentina by IFN-gamma ELISPOT assays and immunoblotting. Positive T. cruzi antigen-induced IFN-gamma responses were detected in 42% of subjects evaluated (15/26 positive...
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PMID: 20123034
PDF is available here.
PDF is available here.
Abstract
The precise function of Plasmodium falciparum histidine-rich protein II (HRPII) is not known, but in vitro studies have indicated its immunomodulatory function. The host immune response to HRPII was measured and compared with the response to P. falciparum merozoite surface protein 1 (MSP1) in four g...
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PMID: 20116080
PDF is available here.
PDF is available here.
Parasitology Research (106)6 2010
Abstract
We conclude that specific antibodies are able to induce agglutination and apoptosis in epimastigotes, although the pathway is not elucidated....
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PMID: 20237802
PDF is available here.
PDF is available here.
Abstract
We present data on the development of enzyme-linked immunosorbent assay (ELISA)-based techniques for the detection of antibodies against the recombinant protein Leishmania infantum cytosolic tryparedoxin peroxidase (LicTXNPx) and a comparison of the results with those employing soluble Leishmania an...
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PMID: 20164286
PDF is available here.
PDF is available here.
Veterinary Parasitology (169)1-2 2010
Abstract
We propose the possibility of an olfactory nerve pathway route of infection for S. neurona meningoencephalitis....
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PMID: 20106599
PDF is available here.
PDF is available here.
Veterinary Parasitology (169)1-2 2010
Abstract
I and III, GRA7I for strains type I and GRA7III for strains type III. As reference material, and to validate this approach, serum samples from eleven free-range chickens and fifteen pigs used for Toxoplasma genotypes isolation were selected. These strains integrate the Biological Resource Centre (BR...
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PMID: 20083355
PDF is available here.
PDF is available here.
Veterinary Parasitology (169)1-2 2010
Abstract
The zoonoses toxoplasmosis and leishmaniasis are important worldwide and also affect wild animals. Thus, the present study aimed to assess the prevalence of Leishmania spp. and Toxoplasma gondii antibodies in 52 serum samples from captive crab-eating foxes (Cerdocyon thous) kept in 17 zoos in São P...
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PMID: 20060648
PDF is available here.
PDF is available here.
Rinsho Shinkeigaku (50)4 2010
Abstract
We report a 60-year-old woman with toxoplasma encephalopathy. She was being treated with prednisolone and methotrexate for rheumatoid arthritis that was diagnosed at the age of 40. In a preoperative examination of her left fifth finger ganglion, pericardial effusions, cardiomegaly, and a right atria...
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PMID: 20411808
PDF is available here.
PDF is available here.
Abstract
In this largest reported series of FHC patients, we detected a strong association between FHC and intraocular antibody synthesis against rubella virus. Furthermore, in 11 patients, it was possible to confirm an additional intraocular antibody synthesis, in particular HSV. PCR-positive results in the...
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PMID: 19937050
PDF is available here.
PDF is available here.
Abstract
In infection-naïve rabbits Toxoplasma-specific antibodies were detected 10 to 15 days after systemic and ocular infection. Serum antibodies against retinal S-antigen and peptide 35 were not detected in response to systemic Toxoplasma infection. After ocular challenge, aqueous-humour levels of antib...
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PMID: 19956968
PDF is available here.
PDF is available here.
Abstract
We report the intracellular distribution of a novel L. donovani secretory serine protease in the flagellar pocket, as determined by immunogold labeling. Flow cytometry and confocal immunofluorescence analysis revealed that the expression of the protease diminishes sequentially from virulent to atten...
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PMID: 20106998
PDF is available here.
PDF is available here.
Abstract
Parasitology (137)4 2010
Abstract
We described the protective action of the immunomodulatory extract of Kalanchoe pinnata (Kp) in murine and human cutaneous leishmaniasis. In the present study, we investigated the effectiveness of Kp against visceral leishmaniasis, using the BALB/c mouse model of infection with Leishmania chagasi. M...
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PMID: 19961648
PDF is available here.
PDF is available here.
Abstract
Formalin-killed promastigotes (FKP) of Leishmania major, in combination with Montanide ISA 720 (MISA), BCG or alum were used in vaccination of an inbred murine model against cutaneous leishmaniasis (CL). Significant and specific increases in anti-FKP IgG responses were detected for both alum-FKP and...
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PMID: 20464130
PDF is available here.
PDF is available here.
Abstract
We studied each of these peptides individually, as well as in four different combinations, as Multiple Antigen Peptides (MAP), aiming to establish a reliable profile for the acute/recent toxoplasmosis with only one patient serum sample. The diagnostic performance of the assay using MAP1, with the co...
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PMID: 20464125
PDF is available here.
PDF is available here.
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases (28)2 2010
Abstract
Pf-LDH gene was same to reference sequences(DQ198262), while there is a single nucleotide difference at the position 666 between Pv-LDH gene and reference sequences (DQ060151). The coding region of the two genes contained 951 bp encoding a 316-amino-acid residue. Compared with Pf-LDH, Pv-LDH showed...
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PMID: 20666310
PDF is available here.
PDF is available here.
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases (28)2 2010
Abstract
The recombinant plasmids pEASY-E1-LTB were constructed. The specific IgG (0.626/- 0.100) and IgA antibodies (1.086 +/- 0.138) in serum, sIgA (0.886 +/- 0.164) in mucosa douche, cytokines IFN-gamma [(2017 +/- 266) pg/ml] and IL-4 [(203.31) pg/ml] in splenocyte culture supernatant in group D were all...
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PMID: 20666314
PDF is available here.
PDF is available here.
Medical Journal of Australia (192)7 2010
Abstract
Le infezioni in medicina : rivista periodica di eziologia, epidemiologia, diagnostica, clinica e terapia delle patologie infettive (18)1 2010
Abstract
We report a case of acquired toxoplasmosis in an immunocompetent healty 32-year-old man, presenting as acute pericarditis and myocarditis. The patient complained of intense chest pain, asthenia, arthralgia, low-grade fever, neck lymphadenopathy. Increased seric cardiac enzymes, electrocardiografic a...
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PMID: 20424527
PDF is available here.
PDF is available here.
Abstract
We developed a neutralization assay using 80 microg/ml of a soluble membrane fraction of Toxoplasma in the automated Elecsys IgG assay. We were able to block the detection of Toxoplasma IgG antibodies in 283 sera with IgG titers ranging from below the limit of detection to >2,300 IU/ml. The mean per...
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PMID: 20151168
PDF is available here.
PDF is available here.
Abstract
An understanding of the nature of the immune response to asexual erythrocytic stages of malaria parasites will facilitate vaccine development by identifying which responses the vaccine should preferentially induce. The present study examined and compared the immune responses of NIH mice in either si...
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PMID: 20333282
PDF is available here.
PDF is available here.
Parasitology (137)2 2010
Abstract
Recombinant NcPDI(recNcPDI), NcROP2(recNcROP2), and NcMAG1(recNcMAG1) were expressed in Escherichia coli and purified, and evaluated as potential vaccine candidates by employing the C57Bl/6 mouse cerebral infection model. Intraperitoneal application of these proteins suspended in saponin adjuvants l...
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PMID: 19835644
PDF is available here.
PDF is available here.
Abstract
The domesticated and partially domesticated dogs living in Garanhuns present anti-Leishmania spp antibodies, but are mostly asymptomatic....
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PMID: 20305967
PDF is available here.
PDF is available here.
Abstract
Among the 80 female students, 53 (66.2%) would be susceptible to toxoplasmosis in a possible pregnancy. Twenty-seven of the female students (33.8%) were considered seropositive....
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PMID: 20305970
PDF is available here.
PDF is available here.