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Immune Sera (1):
Recent article
Immune Sera
Abstract
We cloned genes coding for human HMGB1 and its active components A box and B box by PCR and inserted the cloned genes into pET28a vectors for transformation of Escherichia coli BL21. The E. coli expressed proteins were then purified with a Ni(2+)-NTA column and the endotoxin content was removed. Rec...
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PMID: 21255697
PDF is available here.
PDF is available here.
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Abstract
We developed a new trivalent inactivated vaccine, Kyoto Biken Feline-CPR, consisting of three FCV strains; one was the production strain of our previous vaccine, and the others were screened from 60 field isolates obtained between 1998 and 2000 based on cross-neutralization tests. In this report, th...
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PMID: 20453450
PDF is available here.
PDF is available here.
Clinical Immunology (136)2 2010
Abstract
We wondered if this molecule might modify immune-mediated glomerular disease through its functions as a receptor for apoptotic cells and immunoregulatory molecule. Mer-knockout (KO) mice showed decreased survival rate and greatly increased proteinuria and serum urea levels compared to wild type (WT)...
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PMID: 20444650
PDF is available here.
PDF is available here.
Abstract
We developed and validated a Salmonella flagella microagglutination test (SALMATcor) that significantly reduces laboratory requirements of antisera. SALMATcor is based on scaling down, by fivefold, the antigen:antiserum volumes actually required for the reference method: flagella standard tube agglu...
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PMID: 20367066
PDF is available here.
PDF is available here.
Journal of Virology (84)13 2010
Abstract
We determined that convalescent immune plasma from a horse persistently infected with equine infectious anemia virus (EIAV) neutralized homologous virus and several envelope variants containing heterologous principal neutralizing domains (PND). Plasma was infused into young horses (foals) affected w...
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PMID: 20392850
PDF is available here.
PDF is available here.
Abstract
We describe a series of peptide mimetics of the gp41 prehairpin intermediate designed to permit a systematic analysis of the immune response generated in animals. To improve the chances of detecting weak neutralizing polyclonal responses, two strategies were employed in the initial screening: use of...
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PMID: 20483992
PDF is available here.
PDF is available here.
Abstract
Human pulmonary and subcutaneous dirofilariosis caused by Dirofilaria immitis and Dirofilaria repens are worldwide diagnosed with increasing frequency. These species are responsible for the development of benign pulmonary and subcutaneous nodules, respectively, that can be confused with lung or cuta...
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PMID: 20197111
PDF is available here.
PDF is available here.
Abstract
We were looking for an in vitro BBB model expressing these receptors and studied the alteration of transport of Abeta peptides across this model under the influence of immunoglobulins. Cell line ECV304 was found to be suitable for our experiments. We found evidence for involvement of an improved cle...
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PMID: 20432623
PDF is available here.
PDF is available here.
Critical Care Medicine (38)4 Suppl 2010
Abstract
The world is experiencing a pandemic of swine-origin influenza virus H1N1. A vaccine to prevent disease is now available, and millions have or will become ill before they can be vaccinated. The ability to use swine-origin influenza virus vaccines as a public health tool has been described as a "race...
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PMID: 20154602
PDF is available here.
PDF is available here.
Critical Care Medicine (38)4 Suppl 2010
Abstract
Influenza is a seasonal viral infection associated with significant morbidity and mortality. In 2009, a novel H1N1 influenza A virus emerged and has been classified as a pandemic. In contrast to seasonal influenza, severe disease from pandemic H1N1 seems concentrated in older children and young adul...
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PMID: 19935413
PDF is available here.
PDF is available here.
Abstract
Nociceptin/orphanin FQ (N/OFQ), added in vitro to murine spleen cells in the picomolar range, suppressed antibody formation to sheep red blood cells in a primary and a secondary plaque-forming cell assay. The activity of the peptide was maximal at 10(-12) M, with an asymmetric U-shaped dose-response...
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PMID: 20119853
PDF is available here.
PDF is available here.
Abstract
The mitochondrial ATP-dependent K channel (mitoKATP) has been shown to play a role in cellular protection against apoptosis, or programmed cell death. This channel has been identified and characterized in a number of cell and tissue types but to date the possible existence of mitoKATP in osteoblasti...
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PMID: 20036918
PDF is available here.
PDF is available here.
Abstract
I pyrethroids without a cyano group, the second (hapten 2 and XQ) for type II pyrethroids with a cyano group, and the third (hapten 4 and 5) for both types of pyrethroids with loss of the ester group. The hapten structures were confirmed by MS and 1H-NMR. Hapten 1 and 2 were conjugated with BSA resp...
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PMID: 20110881
PDF is available here.
PDF is available here.
Neuroimmunomodulation (17)4 2010
Abstract
LINGO-1 (leucine-rich repeat and Ig domain-containing, Nogo receptor-interacting protein) is an important component of the NgR receptor complex involved in RhoA activation and axon regeneration. The authors report on passive immunization with LINGO-1 polyclonal antiserum, a therapeutic approach to o...
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PMID: 20203533
PDF is available here.
PDF is available here.
Tsitologiia (52)5 2010
Abstract
We suggested that antibodies developed against DT can cross react with EGFR. Here, using serum from healthy donors (n = 10) and diphtheria patients (n = 15), we demonstrated that B-subunit of DT has the antigenic epitopes similar to those of EGFR. Diphtheria toxin as well as EGFR could be recognized...
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PMID: 20586270
PDF is available here.
PDF is available here.
Canadian Journal of Physiology and Pharmacology/Revue Canadienne de Physiologie et Pharmacologie (88)1 2010
Abstract
These results indicate that EDLC may participate in depressing Na+/K+-ATPase activity and sodium pump alpha isoform gene expression in I/R heart. It is suggested that treatment with ADA may prevent EDLC-mediated reperfusion injury via modulating sodium pump isoform gene expression....
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PMID: 20130737
PDF is available here.
PDF is available here.
Abstract
It was shown that antibodies specific to studied polypeptides opsonized several strains of group A and B streptococci as well as pneumococci. Immunization of mice with ScpB1 polypeptide resulted in more rapid recovery of animals from challenge systemic group B streptococcal infection. Antisera speci...
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PMID: 20465000
PDF is available here.
PDF is available here.
Abstract
Parasitology (51)3-4 2009
Abstract
Parasitology (89 ( Pt 2))02 2009
Abstract
Immunity to Hymenolepis nana was adoptively transferred to recipient mice of 4 strains using mesenteric lymph node cells (MLNC), spleen cells (SC), and/or serum taken from infected donor mice. Donor cells capable of transferring immunity appeared in the mesenteric lymph nodes and spleen at an early...
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PMID: 6542201
PDF is available here.
PDF is available here.
Abstract
Livestock animals have made a significant contribution to human health and well-being throughout humankind's history. A significant contribution of farm animals to human health are the longstanding use of bovine and porcine for production of insulin (for treatment of diabetes), gelatin (for pharmaceu...
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PMID: 19591041
PDF is available here.
PDF is available here.
Abstract
We developed a new approach to identify polyvalent vaccine candidates from outer membrane (OM) proteins of Vibrio alginolyticus with the ability to fight against infections caused by different genera and families of Gram-negative bacteria. This approach combined heterogeneous antiserum-based immunop...
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PMID: 19640004
PDF is available here.
PDF is available here.
Parasitology (136)11 2009
Abstract
We investigated the mechanisms of the Rhipicephalus (Boophilus) microplus saliva anti-haemostatic activity and the possible relationship between the acquired natural anti-tick host resistance and anti-haemostatic action. For this purpose, we studied the effects of R. microplus saliva on different pa...
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PMID: 19627637
PDF is available here.
PDF is available here.
Avian Diseases (53)3 2009
Abstract
Duck enteritis virus (DEV) causes substantial losses on duck farms; however, its molecular biology is poorly understood. Here, an open reading frame of a US3-like gene of DEV was identified from a DEV genomic library. Its existence was confirmed by cloning from DEV-infected duck embryo fibroblasts (...
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PMID: 19848073
PDF is available here.
PDF is available here.
Journal of Autoimmunity (33)1 2009
Abstract
We conclude that the recombinant preparation of 21OH presented here is of sufficient purity and quality to be used for studies of cellular and humoral immunity in autoimmune Addison's disease....
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PMID: 19329278
PDF is available here.
PDF is available here.
Pediatric Emergency Care (25)8 2009
Abstract
We report a case of a 7-year-old girl bitten by a macaque on her forehead and discuss her postexposure management....
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PMID: 19687714
PDF is available here.
PDF is available here.
Abstract
Molecular and Cellular Biochemistry (328)1-2 2009
Abstract
We previously demonstrated that Mipu1 was a nuclear protein and a transcriptional repressor. In this study, Mipu1 was expressed in E. coli and purified using a recombinant expression system and a purification protocol. Milligram quantities of highly purified Mipu1 were obtained. The purified protein...
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PMID: 19337696
PDF is available here.
PDF is available here.
Parasitology (54)03 2009
Abstract
Abstract
We cloned two variants of myo3B from mouse retina and determined that they are expressed early in retinal development. In this study we show for the first time in a mammal that both Myo3B and Myo3A proteins are present in inner segments of all photoreceptors. Myo3B is also present in outer segments...
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PMID: 19332056
PDF is available here.
PDF is available here.
Current protocols in neuroscience / editorial board, Jacqueline N. Crawley ... [et al.] (Chapter 5) 2009
Abstract
Much of modern biology and biochemistry relies on the availability of highly specific antibodies for use in such ubiquitous techniques as immunohistochemistry, ELISAs, immunoprecipitation, and immunoblotting. Thus, the generation of large quantities of specific antibodies directed against proteins o...
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PMID: 19575471
PDF is available here.
PDF is available here.
Abstract
Our study concludes that microbial infections do have some pathogenic role in causing SSA....
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PMID: 19396603
PDF is available here.
PDF is available here.
Abstract
We sought to identify proteins in the Bacillus anthracis spore, conserved in other strains of the closely related Bacillus cereus group, that elicit an immune response in mammals. Two high throughput approaches were used. First, an in silico screening identified 200 conserved putative B. anthracis s...
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PMID: 19208616
PDF is available here.
PDF is available here.
Abstract
PCR, restriction endonuclease digestion and sequence analyses showed that the Id-2 gene had been correctly inserted into pGEX-6P-1 vector, and the GST-Id-2 fusion protein expressed had a relative molecular mass of approximately 40,000 as shown by SDS-PAGE. The polyclonal antibodies obtained from the...
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PMID: 19726331
PDF is available here.
PDF is available here.
Parasitology (94 ( Pt 3))03 2009
Abstract
All serum transfers from donor rats or rabbits given single or multiple vaccinations of ultraviolet (u.v.)-attenuated Schistosoma japonicum cercariae conferred significant resistance against challenge to mice. Donors given 5 vaccinations, however, produced the most effective sera; rat sera giving up...
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PMID: 3112703
PDF is available here.
PDF is available here.
Parasitology (94 ( Pt 3))03 2009
Abstract
The role of different antibody isotypes in the transfer of protection with serum from rats vaccinated with irradiated cercariae were investigated by immunoadsorption of IgG, IgG2a and IgG2c, and by heating at 56 degrees C to remove IgE. Only depletion of IgG2a reduced the levels of vaccine-induced i...
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PMID: 3112704
PDF is available here.
PDF is available here.
Abstract
The sequencing assay showed that the cloned pV gene was highly homologous with that of Ad41 Tak strain, and there were only three residues changed in the corresponding amino-acid sequence. pV was expressed as inclusion bodies or in soluble form in BL21(DE3) cells under inducing condition of 1 mmol/L...
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PMID: 19637578
PDF is available here.
PDF is available here.
Glycoconjugate Journal (26)4 2009
Abstract
Because anti-glycolipid antibodies are involved in the onset of several neurological diseases, the reactivity of glycolipids on erythrocytes and the probability of generating the antibodies were determined to clarify the contribution of glycolipids as antigens. Anti-erythrocyte antisera reacted with...
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PMID: 18923900
PDF is available here.
PDF is available here.
Abstract
Our preceding studies have reported that 2,2',5,5'-tetrachlorobiphenyl (tetraCB)(CB52) is mainly metabolized to 3-hydroxy (OH)-metabolite by phenobarbital (PB)-inducible cytochrome P450 (P450) isoforms such as CYP2B1 and CYP2B18. In this study, the metabolism of CB52 by liver microsomes of untreated...
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PMID: 19588850
PDF is available here.
PDF is available here.
Euro surveillance : bulletin europeen sur les maladies transmissibles = European communicable disease bulletin (14)13 2009
Abstract
Rabies is a lethal encephalitis caused by a lyssavirus and transmitted from animals to humans via bite wound, scratch wound, or licking of mucous membranes. It is preventable by timely administration of post-exposure prophylaxis (PEP) consisting of four or five doses of rabies vaccine combined, in t...
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PMID: 19341608
PDF is available here.
PDF is available here.
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (25)4 2009
Abstract
The recombinant prokaryotic expression vector pET32a-Amelotin was constructed and the recombinant prorein was purified successfully. ELISA analysis showed the titer of the generated antiserum was 1:12,800. Western blot and immunohistochemistry analysis demonstrated this antibody bound specifically w...
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PMID: 19351503
PDF is available here.
PDF is available here.
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (25)4 2009
Abstract
The titer of the antiserum determined by ELISA was up to 1:128,000. The kaff value of the antibody was 8.96 x 10(-6) mol/L. Western blot analysis showed the antibody was of high specificity. The final purified antibody was highly specific to native form of APE1 protein in mice and rats. CONCLUSION:...
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PMID: 19351502
PDF is available here.
PDF is available here.
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (25)4 2009
Abstract
Myostatin gene vaccine could induce the anti-serum against Myostatin in immunized mice. Compared with that in the control group, the mean weight in the pVAC-TT-Ms immunized mice group increased with 9.8%. The quadriceps muscle, gastrocnemius muscle and pectoralis magor in immunized mice increased wi...
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PMID: 19351500
PDF is available here.
PDF is available here.
Parasitology (92 ( Pt 2))02 2009
Abstract
Electrophoresis of extracts of schizonts of Plasmodium knowlesi in non-dissociating polyacrylamide gels, separates several bands of acid endopeptidase activity. A polyclonal antiserum, produced by immunization with purified merozoites, failed to distinguish between different bands of the parasite en...
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PMID: 3520446
PDF is available here.
PDF is available here.
Abstract
We propose that receptor antibodies need better validation before they can reliably be used....
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PMID: 19198807
PDF is available here.
PDF is available here.
Biochemical Journal (419)1 2009
Abstract
We screened a phage display library for peptides recognized by one such antibody (8H5). We analysed the specificity of 8H5 for reactive peptides presented as fusion proteins of HBc (hepatitis B core protein) and HEV (hepatitis E virus) structural protein, p239. This was then related to the specifici...
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PMID: 18973474
PDF is available here.
PDF is available here.
Abstract
Artemisinin is an endoperoxide sesquiterpene lactone isolated from the Chinese medicinal plant Artemisia annua L. It has been widely used in South-East Asia and Africa as an effective drug against sensitive and multidrug-resistant Plasmodium falciparum malaria. A monoclonal antibody...
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PMID: 19066864
PDF is available here.
PDF is available here.
Proteomics (9)4 2009
Abstract
The blood-brain barrier (BBB) is a vascular endothelial interface that separates the brain interior from the bloodstream. Membrane proteins resident at the BBB play important functional and regulatory roles. The current study describes the development and successful implementation of...
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PMID: 19180536
PDF is available here.
PDF is available here.
Abstract
These results suggest that vaccination with SEAD227A, which is devoid of toxic properties, provides protection against SEA-induced emesis. This nontoxic mutant and its specific antibodies might be useful in the prevention and treatment of staphylococcal food poisoning....
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PMID: 19133806
PDF is available here.
PDF is available here.
BMC Genomics (10)1 2009
Abstract
A total of 1438 ESTs were generated and grouped into 611 clusters. Toxin transcripts represented 46% of the total ESTs. The two main toxin classes consisted of three-finger toxins (3FTx) (24%) and phospholipases A(2) (PLA(2)s) (15%). However, 8 other classes of toxins were present, including C-type...
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PMID: 19291316
PDF is available here.
PDF is available here.
Abstract
Overall, our data support the hypothesis that contrary to previous work in rodents, very early exposure of neonatal pigs to a single small dose of antigen can reduce subsequent immune responses. This may have implications for human health. However, although these data point to a reducing/regulatory...
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PMID: 19439986
PDF is available here.
PDF is available here.
Abstract
This chapter describes a method for the generation of polyclonal antibodies against bacteriophages and how these may be assayed immunochemically and biologically.
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PMID: 19066828
PDF is available here.
PDF is available here.
Abstract
The entire ORF of the UL31 was cloned into pET 32a (+) prokaryotic expression vector. Escherichia coli BL21(DE3) competent cells were transformed with the construct followed by the induction of protein expression by the addition of IPTG. Band corresponding to the predicted sizes (55 kDa) was produce...
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PMID: 19208242
PDF is available here.
PDF is available here.
Autoimmunity (42)3 2009
Abstract
I muscle fibers. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and precise molecular weight derived from mass spectrometer identified P25 as carbonic anhydrase III (CA III). Some members of CA family are related to autoimmune diseases and CA III is recently reported to...
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PMID: 19301202
PDF is available here.
PDF is available here.
Abstract
Crude antigenic preparations from heterologous filarial parasites gave false positive results because of complex nature of these antigens and their cross-reactivity with other helminth parasites. In the present study, efforts have been made to isolate and characterize the antigens from Setaria cervi...
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PMID: 19860586
PDF is available here.
PDF is available here.
Voprosy Virusologii (54)1 2009
[Cytotoxic properties of diagnostic rabbit sera to enteroviruses specific features and localization]
Abstract
Hyperimmune rabbit enterovirus-neutralizing diagnostic sera have a pronounced cytotoxic effect on the test tissue culture RD, HEp-2, and Vero cells and primary renal cells from African green monkeys (Cercopithecus aethiops). The specific features of this developing toxic effect, such as reduced prol...
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PMID: 19253727
PDF is available here.
PDF is available here.
Public Health Reports (124)2 2009
Abstract
We utilized the smallpox post-event clinic plans of the Centers for Disease Control and Prevention (CDC), although the plans had only been exercised for smallpox. Following CDC's guidelines for staffing and organizing the mass clinic, we provided 5,038 doses of ISG during a total of 24 hours of clin...
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PMID: 19320362
PDF is available here.
PDF is available here.
Abstract
Immunogenic properties of recombinant protein ScaAB mixed with polypeptide P6 as well as conjugate of the former with capsular polysaccharide type III (PSIII) were assessed. Protein-polysaccharide conjugate was synthesized by reductive amination method. Immunogenicity was studied on the mouse model...
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PMID: 19338234
PDF is available here.
PDF is available here.
Abstract
It was established that increase of Yersinia pseudotuberculosis cultivation temperature from 10 degrees C to 37 degrees C was associated with attenuation of LPS toxicity in guinea pigs, decrease of its immunochemical activity and contents and degree of acylation of 3OH-14:0 hydoxyacid as well as amo...
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PMID: 19459469
PDF is available here.
PDF is available here.
Experimental Parasitology (121)1 2009
Abstract
We extracted Schistosoma mansoni tegumental microtubule associated proteins utilizing detergent/high-salt procedure and raised antiserum against these proteins. The antiserum was applied to screen Schistosoma haematobium lambda gt11 expression library and some of the isolated clones were sequenced....
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PMID: 18996374
PDF is available here.
PDF is available here.