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Centromere Protein B (0):
Recent article
Centromere Protein B
EMBO Journal (29)20 2010
Abstract
We show that the temporal regulation of kMT attachments by CLASP1, astrin and Kif2b is central to mitotic progression and chromosome segregation fidelity. In early mitosis, a Kif2b-CLASP1 complex is recruited to kinetochores to promote chromosome movement, kMT turnover, correction of attachment erro...
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PMID: 20852589
PDF is available here.
PDF is available here.
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Future Oncology (6)3 2010
Abstract
In total, 55 patients with breast cancer were prospectively included in the study. Median follow-up time was 62 months. In univariate analysis, estrogen receptor status, anti-CENP-B antibody status and tumor size significantly affected disease-free survival, whereas overall survival was significantl...
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PMID: 20222802
PDF is available here.
PDF is available here.
Abstract
Our data suggest that anti-CENP-A antibodies are a more specific biomarker for SSc than antibodies to CENP-B. Furthers studies are required to verify these findings....
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PMID: 20487535
PDF is available here.
PDF is available here.
Abstract
Journal of Microscopy (Oxford) (231)Pt 1 2008
Abstract
We introduce the CLEM factor as a quantitative measure of reduction in phototoxicity and photobleaching. Finally, we give a guideline to optimize the effect of CLEM without compromising image quality....
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PMID: 18638185
PDF is available here.
PDF is available here.
Abstract
We measured the effects of eribulin on dynamics of centromeres and their attached kinetochore microtubules by time-lapse confocal microscopy in living mitotic U-2 OS human osteosarcoma cells. Green fluorescent protein-labeled centromere-binding protein B marked centromeres and kinetochore-microtubul...
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PMID: 18645010
PDF is available here.
PDF is available here.
Medicine (Baltimore) (87)3 2008
Abstract
We assess whether audiovestibular involvement is present in patients with limited scleroderma (lSSc). To answer this question we studied a series of 35 consecutive patients who fulfilled well-established classification criteria for lSSc and had antibodies against the major centromere protein-B (CENP...
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PMID: 18520322
PDF is available here.
PDF is available here.
Lung Cancer (60)2 2008
Abstract
We report on a small-cell lung cancer case that was heralded by the presence of CENP-B specific anti-centromere autoantibodies (ACA) detected well before the diagnosis of cancer. The patient received chemotherapy plus radiotherapy, which resulted in complete remission of the tumor. Serum ACA levels...
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PMID: 17980453
PDF is available here.
PDF is available here.
EMBO Journal (27)7 2008
Abstract
We show that the fission yeast homologue of CENP-B, Abp1, binds to the silent domain of the mating-type locus and regulates directionality of switching. Deletion of abp1 prevents utilisation of mat2P, as when heterochromatin is disrupted and spreading of Swi2-Swi5 is impaired. Our results show that,...
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PMID: 18354497
PDF is available here.
PDF is available here.
Journal of Cell Biology (180)6 2008
Abstract
We have assessed the exchange rates of inner centromere proteins (CENPs) by quantitative microscopy throughout the cell cycle in human cells. CENP-A and CENP-I are stable centromere components that are incorporated into centromeres via a "loading-only" mechanism in G1 and S phase, respectively. A su...
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PMID: 18347072
PDF is available here.
PDF is available here.
Abstract
Abstract
Abstract
We describe a genome surveillance mechanism for retrotransposons by transposase-derived centromeric protein CENP-B homologues of the fission yeast Schizosaccharomyces pombe. CENP-B homologues of S. pombe localize at and recruit histone deacetylases to silence Tf2 retrotransposons. CENP-Bs also repre...
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PMID: 18094683
PDF is available here.
PDF is available here.
ChemBioChem (9)1 2008
Abstract
We investigated the association of CENP-A and CENP-B by fluorescence intensity and lifetime-based FRET measurements in living human HEp-2 cells. We observed Förster resonance energy transfer (FRET) between CENP-A and CENP-B at centromere locations; this indicates that these proteins are in the mole...
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PMID: 18072184
PDF is available here.
PDF is available here.
Abstract
Abstract
We took advantage of the vast amount of sequence information that has become recently available for a wide range of fungal and animal species to investigate the origin of the mammalian CENP-B and yeast CENP-B-like genes. A highly conserved ortholog of CENP-B was detected in 31 species of mammals, in...
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PMID: 17940212
PDF is available here.
PDF is available here.
Abstract
We demonstrate here that de novo centromere assembly on HAC/MAC is dependent on CENP-B. In contrast, centromere formation is suppressed in cells expressing CENP-B when alpha-satellite DNA was integrated into a chromosomal site. Remarkably, on those integration sites CENP-B enhances histone H3-K9 tri...
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PMID: 18160038
PDF is available here.
PDF is available here.
Arthritis & Rheumatism (56)11 2007
Abstract
CENP-B was found to bind specifically to the surface of human pulmonary artery smooth muscle cells (SMCs) and not to fibroblasts or endothelial cells (ECs). Furthermore, CENP-B bound preferentially to SMCs of the contractile type rather than to SMCs of the synthetic type. Binding of CENP-B to SMCs s...
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PMID: 17968937
PDF is available here.
PDF is available here.
Abstract
We demonstrated on the mouse cultured cell line and prematurely condensed chromosomes that: (a) all four mouse satellite DNA fragments (major and minor satellite, mouse satellite 3 (MS3) and mouse satellite 4 (MS4)) were involved in the thread formation; (b) MS4 was involved in the thread to the lea...
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PMID: 17340617
PDF is available here.
PDF is available here.
Journal of Cell Biology (177)5 2007
Abstract
We have termed interphase centromere damage response (iCDR), was observed in all tested human and mouse cells, indicative of a conserved mechanism. Knockdown cells for several constitutive centromere proteins have shown that the loss of centromeric protein B provokes the centromeric accumulation of...
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PMID: 17548509
PDF is available here.
PDF is available here.
Abstract
The location of centromeric protein CENP-B and telomeric protein TRF2/MTBP in the mouse spermatogenic line has been studied using indirect immunofluorescent and immunoelectron microscopy. CENP-B localized to the heterochromatic parts of the nuclei at meiotic stages. A clearly distinct chromocenter f...
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PMID: 17353134
PDF is available here.
PDF is available here.
EMBO Journal (26)5 2007
Abstract
Chromatin clusters containing CENP-A, a histone H3 variant, are found in centromeres of multicellular eukaryotes. This study examines the ability of alpha-satellite (alphoid) DNA arrays in different lengths to nucleate CENP-A chromatin and form functional kinetochores de novo. Kinetochore assembly w...
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PMID: 17318187
PDF is available here.
PDF is available here.
FEBS Letters (581)4 2007
Abstract
The ICP0 protein of herpes simplex virus type 1 (HSV-1) is a nuclear protein that possesses a well-characterized E3 ubiquitin ligase activity. This activity is responsible for the proteasomal-dependent degradation of several cellular proteins. This study shows that ICP0 induces the proteasomal-depen...
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PMID: 17258208
PDF is available here.
PDF is available here.
Current Biology (17)3 2007
Abstract
We show by quantitative fluorescence measurements in living early embryos that functional fluorescent fusion proteins of the Drosophila CENP-A and CENP-C homologs are rapidly incorporated into centromeres during anaphase. This incorporation is independent of ongoing DNA synthesis and pulling forces...
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PMID: 17222555
PDF is available here.
PDF is available here.
Chromosoma (115)6 2006
Abstract
We used correlative light (LM) and electron microscopy (EM) to study kinetochores in cultured Drosophila S2 cells during metaphase, and after colchicine treatment to depolymerize all microtubules (MTs). We find that the structure of attached kinetochores in S2 cells is indistinct, consisting of an a...
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PMID: 16909258
PDF is available here.
PDF is available here.
Journal of Autoimmunity (27)4 2006
Abstract
Autoantibodies to centromeric proteins are commonly found in sera of limited scleroderma and other rheumatic disease patients. To better understand the inciting events and possible pathogenic mechanisms of these autoimmune responses, this study identified the common antigenic targets of CENP-A in sc...
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PMID: 17210244
PDF is available here.
PDF is available here.
Abstract
The pattern of CENP recognition differs markedly in pSS and limited scleroderma. While patients with pSS predominantly recognise CENP-C alone, dual recognition of CENP-B and CENP-C is most frequent in scleroderma. These findings suggest that obtaining antibodies to specific centromere antigens is us...
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PMID: 16414973
PDF is available here.
PDF is available here.
Clinical Immunology (120)2 2006
Abstract
We have analyzed the self-reactive repertoires of IgG from 20 patients with limited cutaneous SSc, 40 patients with diffuse SSc and 60 age- and sex-matched healthy controls. Serum IgG from 15/20 patients with limited cutaneous SSc and anti-centromere antibodies bound to at least one of the two 75- a...
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PMID: 16580263
PDF is available here.
PDF is available here.
Chromosome Research (14)7 2006
Abstract
We determined consensus alpha satellite higher-order repeats (HOR) and consensus distributions of CENP-B box and pJalpha motif in human chromosomes 1, 4, 5, 7, 8, 10, 11, 17, 19, and X. We determined new suprachromosomal family (SF) assignments: SF5 for 13mer (2211 bp), SF5 for 13mer (2214 bp), SF2...
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PMID: 17115329
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (280)50 2005
Abstract
We demonstrated centromere-specific nucleosome formation in vitro with recombinant proteins, including histones H2A, H2B, H4, CENP-A, and the DNA-binding domain of CENP-B. The CENP-A nucleosome wraps 147 base pairs of the alpha-satellite sequence within its nucleosome core particle, like the canonic...
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PMID: 16183641
PDF is available here.
PDF is available here.
Abstract
We show that both early-passage Foxm1(-)(/)(-) mouse embryonic fibroblasts (MEFs) and human osteosarcoma U2OS cells depleted of FoxM1 protein by small interfering RNA fail to grow in culture due to a mitotic block and accumulate nuclear levels of cyclin-dependent kinase inhibitor (CDKI) proteins p21...
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PMID: 16314512
PDF is available here.
PDF is available here.
Abstract
These results show a potential therapeutic role for anti-PSCA antibodies in the treatment of pancreatic cancer. Furthermore, PSCA might serve as a novel marker in the diagnosis of pancreatic cancer....
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PMID: 16024997
PDF is available here.
PDF is available here.
Abstract
We have evaluated the performance of a novel line-blot immunoassay (LIA; Mikrogen) and compared results with those obtained by CIE (in-house), ELISA (Pharmacia Diagnostics), and FEIA (Pharmacia Diagnostics). Sera from systemic lupus erythematosus (SLE) patients (n = 123), systemic sclerosis patients...
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PMID: 16014550
PDF is available here.
PDF is available here.
FEBS Letters (579)11 2005
Abstract
We have identified and characterized the first satellite DNA (P.k.SAT) from microbat species Pipistrellus kuhli. The presence of mammalian CENP-B box and yeast CDEIII box could indicate the participation of P.k.SAT in centromere organization....
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PMID: 15848198
PDF is available here.
PDF is available here.
Abstract
We show that clathrin stabilizes fibres of the mitotic spindle to aid congression of chromosomes. Clathrin bound to the spindle directly by the amino-terminal domain of clathrin heavy chain. Depletion of clathrin heavy chain using RNA interference prolonged mitosis; kinetochore fibres were destabili...
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PMID: 15858577
PDF is available here.
PDF is available here.
Abstract
Biology of Reproduction (72)2 2005
Abstract
This study was conducted to examine expression of centromere protein B (CENPB), spindle checkpoint protein MAD2 (mitotic arrest deficient protein), and antiapoptotic protein BCL2; activities of MAPK (mitogen-activated protein kinase) and mitochondria distribution in pig oocytes during aging, and the...
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PMID: 15469999
PDF is available here.
PDF is available here.
Abstract
A close relationship between autoimmunity and malignant diseases has been supposed for a long time. In clinical practice, anti-SS-B and anti-CENP-B antibodies are used as serologic markers for autoimmune diseases. In this study, anti-SS-B and anti-CENP-B autoantibodies were studied in breast cancer...
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PMID: 15739023
PDF is available here.
PDF is available here.
Nucleic Acids Research (33)2 2005
Abstract
We have created synthetic chromosome 17-derived alpha-satellite arrays, based on the 16-monomer repeat length typical of natural D17Z1 arrays, in which the consensus CENP-B box elements are either completely absent (0/16 monomers) or increased in density (16/16 monomers) compared to D17Z1 alpha-sate...
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PMID: 15673719
PDF is available here.
PDF is available here.
Abstract
Reproduction (127)3 2004
Abstract
These results suggest that Cenpb may play an important function in the short- and long-term maintenance of uterine integrity....
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PMID: 15016956
PDF is available here.
PDF is available here.
Acta Genetica Sinica (31)3 2004
Abstract
We ever got a result that cellular duplicating time was prolonged for another 32.8 h, and together with the inhibition of centromere assembly, the mitotic index dropped sharply. In another research, we drew a conclusion that CenpG may be related to cancer, and its differential expressing probably re...
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PMID: 15195560
PDF is available here.
PDF is available here.
Abstract
We screened a cDNA library by yeast two-hybrid analysis. An interaction between CENP-B and CENP-C was detected, and the CENP-C domains required were determined to overlap with three Mif2 homologous regions, which were also revealed to be involved in the CENP-C assembly of centromeres by expression o...
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PMID: 14612452
PDF is available here.
PDF is available here.
Chromosome Research (12)6 2004
Abstract
The centromere is an essential functional domain responsible for the correct inheritance of eukaryotic chromosomes during cell division. Eukaryotic centromeres include the highly conserved centromere-specific histone H3 variant, CENP-A, which has provided a powerful tool for investigating the recrui...
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PMID: 15289662
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (278)51 2003
Abstract
We determined the structure of the human CENP-B DNA-binding domain, CENP-B-(1-129), complexed with the CENP-B box DNA. In the present study, we determined the crystal structure of its dimerization domain (CENP-B-(540-599)), another functional domain of CENP-B, at 1.65-A resolution. CENP-B-(540-599)...
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PMID: 14522975
PDF is available here.
PDF is available here.
Genes & Development (17)19 2003
Abstract
We identified KNL-1, a novel kinetochore protein whose depletion, like that of CeCENP-A or CeCENP-C, leads to a "kinetochore-null" phenotype. KNL-1 is downstream of CeCENP-A and CeCENP-C in a linear assembly hierarchy. In embryonic extracts, KNL-1 exhibits substoichiometric interactions with CeCENP-...
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PMID: 14522947
PDF is available here.
PDF is available here.
Journal of Cell Science (116)Pt 19 2003
Abstract
We isolated cultured human cell lines in which a transfected alpha-satellite (alphoid) YAC was integrated ectopically into the terminal region of host chromosome 16, where it was stably maintained. Centromere activity of the alphoid YAC was suppressed at ectopic locations on the host chromosome, as...
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PMID: 12953060
PDF is available here.
PDF is available here.
Cancer Research (63)13 2003
Abstract
We demonstrate that the kinetochore protein CENP-A was overexpressed in all of 11 primary human colorectal cancer tissues. CENP-A mRNA was also up-regulated, indicating that overexpression of CENP-A occurred at the transcriptional level. Immunostaining with anti-CENP-A antibodies showed increased CE...
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PMID: 12839935
PDF is available here.
PDF is available here.
Acta Genetica Sinica (30)6 2003
Abstract
We investigated the effects of the anti-leukemia drug Harringtonine (HT) on the levels of centromere proteins and gene expression of centromere protein CenpB in L1210 cells. The intensity of centromere fluorescence, shown by indirect immunofluorescence staining, decreased with HT treatment. Western...
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PMID: 12939796
PDF is available here.
PDF is available here.
Cancer Research (63)11 2003
Abstract
We have tested the hypothesis that a primary mechanism of action of Taxol involves suppression of spindle microtubule dynamics. We have used centromere-binding protein B coupled to green fluorescent protein as a marker for the kinetochores and centromeres of chromosomes and analyzed the effects of l...
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PMID: 12782584
PDF is available here.
PDF is available here.
Plant Cell Online (15)2 2003
Abstract
Clinica Chimica Acta (328)1-2 2003
Abstract
Measurement of anticentromere antibodies by this ELISA using human recombinant antigen is a useful alternative for the autoimmune laboratory checking for diseases associated with anticentromere antibodies.
Copyright 2002 Elsevier Science B.V....
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PMID: 12559609
PDF is available here.
PDF is available here.
Journal of Cell Biology (159)5 2002
Abstract
We created four kinds of synthetic repetitive sequences: modified alphoid DNA with point mutations in all CENP-B boxes, resulting in loss of all CENP-B binding activity; unmodified alphoid DNA containing functional CENP-B boxes; and nonalphoid repetitive DNA sequences with or without functional CENP...
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PMID: 12460987
PDF is available here.
PDF is available here.
Human Molecular Genetics (11)19 2002
Abstract
We report the association of PARP-2 with mammalian centromeres in a cell-cycle-dependent manner, accumulating at centromeres during prometaphase and metaphase, disassociating during anaphase, and disappearing from the centromeres by telophase. Analysis of a pseudodicentric chromosome and a human neo...
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PMID: 12217960
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (277)30 2002
Abstract
We previously described the association of PARP-1 with normal mammalian centromeres and human neocentromeres by affinity purification and immunofluorescence. Here we investigated the interaction of this protein with, and poly(ADP-ribosyl)ation of, three constitutive centromere proteins, Cenpa, Cenpb...
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PMID: 12011073
PDF is available here.
PDF is available here.
Genes & Development (16)14 2002
Abstract
We show that the CENP-B homologs are functionally redundant at centromeres, and that Abp1 binds specifically to centromeric heterochromatin. In the absence of Abp1 or Cbh1, the centromeric association of Swi6 is diminished, resulting in a decrease in silencing of the region. CENP-B-homolog double di...
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PMID: 12130537
PDF is available here.
PDF is available here.
Arthritis & Rheumatism (46)7 2002
Abstract
We describe here the gradual development of an anticentromere immune response in a patient with systemic sclerosis, which started from an antihistone response and was not mediated by molecular mimicry. Via an epitope on histone H3, the antibody response spread to a homologous epitope in the H3 homol...
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PMID: 12124871
PDF is available here.
PDF is available here.
Arthritis & Rheumatism (46)7 2002
Abstract
GB-cleaved autoantigens were immunoblotted by 16 of 19 IDL sera (84.2%) compared with 6 of 15 non-IDL sera (40.0%) (odds ratio 8.0, 95% confidence interval 1.6-40.0). This association persisted after adjustment for ACA status. Furthermore, the presence of antibodies to centromere proteins as well as...
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PMID: 12124872
PDF is available here.
PDF is available here.
Journal of Cell Science (115)Pt 11 2002
Abstract
We took advantage of a chromatin-immunoprecipitation procedure and applied this technique to human HeLa cells. Through this approach we could establish that: (1) CENP-C binds the alpha-satellite DNA selectively; (2) the CENP-C region between amino acids 410 and 537, previously supposed to contain a...
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PMID: 12006616
PDF is available here.
PDF is available here.
Abstract
We have isolated centromeric chromatin containing the CENP-A nucleosome, CENP-B, and CENP-C from HeLa cells using anti-CENP-A and/or anti-CENP-C antibodies and shown that the CENP-A/B/C complex is predominantly formed on alpha-satellite DNA that contains the CENP-B box (alphaI-type array). Mapping o...
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PMID: 11884609
PDF is available here.
PDF is available here.