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G-Box Binding Factors (0):
Recent article
G-Box Binding Factors
B M B Reports (41)2 2008
Abstract
We report that AtbZIP16 and AtbZIP68 could bind cis elements with ACGT core, such as G-box, Hex, C-box and As-1, but with different binding affinities which from high to low were G-box > Hex > C-box > As-1; AtbZIP16 and AtbZIP68 could form homodimer and form heterodimer with other members of G group...
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PMID: 18315949
PDF is available here.
PDF is available here.
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GBF-dependent family genes morphologically suppress the partially active Dictyostelium STATa strain.
Abstract
We have isolated more than 18 putative multicopy suppressors of Dd-STATa using genetic screening. One was hssA gene, whose expression is known to be G-box-binding-factor-dependent and which was specific to prestalk A (pstA) cells, where Dd-STATa is activated. Also, hssA mRNA was expressed in pstA ce...
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PMID: 18204858
PDF is available here.
PDF is available here.
Abstract
We isolated the Daucus carota bZIP1 (DcBZ1) gene encoding a G-box binding factor-type basic region/leucine zipper (GBF-type bZIP) factor from carrot somatic embryos. The expression of DcBZ1 was detected in embryogenic cells, non-embryogenic cells, somatic embryos, developing seeds, seedlings, and tr...
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PMID: 18407508
PDF is available here.
PDF is available here.
Abstract
We show here that the evolutionarily conserved Arabidopsis thaliana protein kinases, KIN10 and KIN11 (also known as AKIN10/At3g01090 and AKIN11/At3g29160, respectively), control convergent reprogramming of transcription in response to seemingly unrelated darkness, sugar and stress conditions. Sensin...
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PMID: 17671505
PDF is available here.
PDF is available here.
Plant Physiology (144)3 2007
Abstract
We analyzed expression of EARLY LIGHT-INDUCIBLE PROTEIN1 (ELIP1), ELIP2, ASCORBATE PEROXIDASE2 (APX2), and LIGHT-HARVESTING CHLOROPHYLL A/B-BINDING PROTEIN2.4 (LHCB2.4) in the phytochrome A (phyA), phyB, cryptochrome1 (cry1), and cry2 photoreceptor mutants and long hypocotyl5 (hy5) and HY5 homolog (...
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PMID: 17478635
PDF is available here.
PDF is available here.
Plant Physiology (144)3 2007
Abstract
We located a 74-bp region within the ORCA3 promoter, which contains an autonomous jasmonate-responsive element (JRE). The ORCA3 JRE is composed of two important sequences: a quantitative sequence responsible for a high level of expression and a qualitative sequence that appears to act as an on/off s...
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PMID: 17496112
PDF is available here.
PDF is available here.
Plant Molecular Biology (64)4 2007
Abstract
We isolated a well-matched sequence homologous to the basic region/helix-loop-helix proteins by yeast one-hybrid screening and named it SebHLH. SebHLH transcripts were expressed in developing seeds and roots of sesame. SebHLH:GFP fusion protein localized in the nucleus. Recombinant SebHLH protein bo...
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PMID: 17420955
PDF is available here.
PDF is available here.
Plant Cell Reports (26)1 2007
Abstract
I elements in the interaction with PsGBF or OsMYC. The results indicated that PsGBF bound with the G-box, but not the Box I element. Moreover, this combination effect of G-box and Box I only associated with PsGBF but not with other bHLH-type proteins such as OsMYC....
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PMID: 16924502
PDF is available here.
PDF is available here.
Abstract
These results provide theoretical basis to the plant defense gene expression mechanism regulated by multiple activators....
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PMID: 17129850
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (281)31 2006
Abstract
We report the identification of a Z-box binding factor (ZBF2) and its functional characterization in cryptochrome-mediated blue light signaling. ZBF2 encodes a G-box binding factor (GBF1), which is a basic leucine zipper transcription factor. Our DNA-protein interaction studies reveal that ZBF2/GBF1...
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PMID: 16638747
PDF is available here.
PDF is available here.
Abstract
We find that salicylic acid and ethephon treatment in hot pepper increases the expression of a putative basic/leucine zipper (bZIP) transcription factor gene, CabZIP1. CabZIP1 mRNA is expressed ubiquitously in various organs. The green fluorescent protein-fused transcription factor, CabZIP1::GFP, ca...
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PMID: 16630556
PDF is available here.
PDF is available here.
Abstract
We showed that overexpression of ethylene-responsive transcription factor (ERF) 2 activated the expression of endogenous genes that have the GCC box in their promoter region, in tobacco plants. These include not only a defense-related gene, CHN50, encoding class I basic chitinase, but also a transcr...
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PMID: 16452120
PDF is available here.
PDF is available here.
Abstract
We imaged SLP-76 and found it in the cytosol of unstimulated cells. Upon FcepsilonRI cross-linking, SLP-76 translocates to the cell membrane, forming clusters that colocalize with the FcepsilonRI, the tyrosine kinase Syk, the adaptor LAT, and phosphotyrosine. The disruption of the SLP-76 interaction...
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PMID: 16479002
PDF is available here.
PDF is available here.
Developmental Biology (290)2 2006
Abstract
We found that the mutant strains developed in suspension with added cAMP expressed the pulse-induced and early adenylyl cyclase (ACA)-dependent genes, but not the later ACA-dependent, post-aggregation genes. Since expression of lagC itself is dependent on GBF, expression of the post-aggregation gene...
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PMID: 16386729
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (280)33 2005
Abstract
We report that, upon interleukin-3 stimulation of Ba/F3 cells, the transcription factor GATA-1 is strongly phosphorylated at residue serine 26 by a MAPK-dependent pathway. Phosphorylation of GATA-1 increases GATA-1-mediated transcription of the E4bp4 survival gene without significantly changing the...
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PMID: 15967790
PDF is available here.
PDF is available here.
Journal of Virology (79)11 2005
Abstract
We have identified a cellular repressor of transcription, activating transcription factor x (ATFx), as a novel Tax-binding protein. In addition to binding directly to Tax we show by electrophoretic mobility shift assay that ATFx binds to the TxRE enhancer element via the bZIP domain. The functional...
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PMID: 15890932
PDF is available here.
PDF is available here.
Biochemistry (Washington) (44)20 2005
Abstract
We have described a miniature protein-based approach to the design of molecules that bind DNA or protein surfaces with high affinity and specificity. In this approach, the small, well-folded protein avian pancreatic polypeptide acts as a scaffold to present and stabilize an alpha-helical or PPII-hel...
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PMID: 15895990
PDF is available here.
PDF is available here.
Molecular Microbiology (56)3 2005
Abstract
Zoospores are critical in the disease cycle of Phytophthora infestans, a member of the oomycete group of fungus-like microbes and the cause of potato late blight. A protein kinase induced during zoosporogenesis, Pipkz1, was shown to interact in the yeast two-hybrid system with a putative bZIP transc...
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PMID: 15819621
PDF is available here.
PDF is available here.
Abstract
Analysis of transcripts of 75 genes encoding putative basic leucine zipper (bZIP) transcription factors in the Arabidopsis genome identified AtbZIP60, which was induced by tunicamycin. AtbZIP60 encodes a predicted protein of 295 aa with a putative transmembrane domain near its C terminus after a bZI...
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PMID: 15781873
PDF is available here.
PDF is available here.
Abstract
We have investigated phylogenies of three families of ancient eukaryotic transcription factors for which fairly reliable interaction data are available. For all three families, bZIP, bHLH and NR (nuclear receptors), we find that homo-dimerising proteins were probably the ancestors and that series of...
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PMID: 15777629
PDF is available here.
PDF is available here.
Abstract
We identify a negative response element located at nucleotides (nts) +96/+105 and demonstrate, using chromatin immunoprecipitation (ChIP) analysis, that in vivo this sequence interacts with the E4BP4 transcriptional repressor. Differences in size and relative abundance of nuclear E4BP4 were observed...
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PMID: 15498853
PDF is available here.
PDF is available here.
EMBO Journal (24)3 2005
Abstract
We show Zip1 is stabilized in pof1 mutants, Pof1 binds only phosphorylated forms of Zip1, and Zip1 is ubiquitylated in vivo, indicating that Zip1 is a substrate of SCF(Pof1). Genome-wide DNA microarray assay shows that many cadmium-induced genes are under the control of Zip1, suggesting Zip1 plays a...
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PMID: 15660136
PDF is available here.
PDF is available here.
Nature Genetics (37)2 2005
Abstract
We identified clock-controlled elements on 16 clock and clock-controlled genes in a comprehensive surveillance of evolutionarily conserved cis elements and measurement of their transcriptional dynamics. Here we report the roles of E/E' boxes, DBP/E4BP4 binding elements and RevErbA/ROR binding elemen...
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PMID: 15665827
PDF is available here.
PDF is available here.
Plant Molecular Biology (57)2 2005
Abstract
We show that in Arabidopsis thaliana all four corresponding genes are coordinately expressed in response to light, and are spatially coexpressed in siliques, flowers and leaves. Light regulatory units (LRUs) sufficient for light responsiveness were identified in all four promoters. Each unit consist...
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PMID: 15821875
PDF is available here.
PDF is available here.
Journal of Molecular Biology (343)4 2004
Abstract
The energetic basis of GCN4-bZIP complexes with the AP-1 and ATF/CREB sites was investigated by optical methods and scanning and isothermal titration microcalorimetry. The dissociation constant of the bZIP dimer was found to be significantly higher than that of its isolated leucine zipper domain: at...
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PMID: 15476806
PDF is available here.
PDF is available here.
Plant Journal (40)1 2004
Abstract
We report that ABF2, an ABF subfamily member of bZIP proteins interacting with the ABA-responsive elements, is involved in ABA/stress responses. Its overexpression altered ABA sensitivity, dehydration tolerance, and the expression levels of ABA/stress-regulated genes. Furthermore, ABF2 overexpressio...
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PMID: 15361142
PDF is available here.
PDF is available here.
Development (131)18 2004
Abstract
We show that E4BP4 is expressed by embryonic rat and chicken motoneurons in vivo, with levels being highest in neurons that survive the period of naturally occurring cell death. Overexpression of E4BP4 by electroporation of purified motoneurons in culture protected them almost completely against cel...
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PMID: 15306565
PDF is available here.
PDF is available here.
Physiological Genomics (18)3 2004
Abstract
We now have evidence suggesting other roles for NHE1, there has been no comprehensive study investigating its role as a signaling molecule. Toward this aim, we used in this study NHE1 null mutant mice and cDNA microarrays to investigate the effects of NHE1 on global gene expression in various region...
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PMID: 15306696
PDF is available here.
PDF is available here.
Endocrinology (145)8 2004
Abstract
We investigate the signaling pathway(s) that lead to PTH induction of E4bp4 mRNA expression. Ten and 100 nm PTH induced maximum E4bp4 expression in MOBs. Forskolin (FSK), an adenylate cyclase inducer, 8-bromo-cAMP, a cAMP analog, and phorbol myristate acetate, a PKC activator, increased E4bp4 mRNA l...
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PMID: 15087429
PDF is available here.
PDF is available here.
Abstract
The quality of wheat grain is largely determined by the quantity and composition of storage proteins (prolamins) and depends on mechanisms underlying the regulation of expression of prolamin genes. The endosperm-specific wheat basic region leucine zipper (bZIP) factor storage protein activator (SPA)...
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PMID: 15284875
PDF is available here.
PDF is available here.
Current Biology (14)11 2004
Abstract
We demonstrate that prolonged light period-dependent accumulation of E4BP4 protein is temporally coordinated with a delay of the rising phase of cPer2 in the morning. E4BP4 was phosphorylated progressively and then disappeared in parallel with induced cPer2 expression. Characterization of E4BP4 reve...
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PMID: 15182670
PDF is available here.
PDF is available here.
Acta Genetica Sinica (31)6 2004
Abstract
A new full-length cDNA clone was isolated from the fiber cDNA library of Gossypium hirsutum L. The cDNA, designated GhbZIP,encoded a polypeptide of 645 amino acids. GhbZIP protein had the structure characteristics of plant bZIP proteins, including two conserved domains, DUF630 and DUF632 with unknow...
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PMID: 15490881
PDF is available here.
PDF is available here.
Abstract
Our data suggests that the overexpression of E4BP4 is unable to block apoptosis induced by IL-3 withdrawal and that the expression of E2A-HLF does not replace the function of E4BP4 in mediating survival....
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PMID: 15147370
PDF is available here.
PDF is available here.
DNA Sequence (15)3 2004
Abstract
A cDNA encoding the first mollusk member of the PAR subfamily of bZIP transcription factors has been characterized in the Pacific oyster, Crassostrea gigas. The sequence of the cDNA predicts a protein of 260 amino acids that has 41-50% identity with the PAR domain, 72-79% identity with the basic DNA...
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PMID: 15497439
PDF is available here.
PDF is available here.
Biochemistry (Washington) (43)16 2004
Abstract
We have characterized the bZIP domain of O2 by nuclear magnetic resonance (NMR), circular dichroism (CD), and size-exclusion chromatography. Results obtained from CD measurements suggested that the native O2bZIP has about 40 of its 49 leucine-zipper residues in helical structure, while the DNA-bindi...
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PMID: 15096055
PDF is available here.
PDF is available here.
Abstract
We examined whether Arabidopsis bZIPs function as transcription factors for the ACTCAT sequence. Transient expression analysis revealed that the four proteins in Group S bZIPs, AtbZIP11/ATB2, AtbZIP44, AtbZIP2/GBF5 and AtbZIP53, formed an ATB2 subgroup that activated expression of the GUS reporter g...
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PMID: 15047879
PDF is available here.
PDF is available here.
Development (131)3 2004
Abstract
We used genetic selection to isolate mutants in the DIF-1 response pathway. The disrupted gene in one such mutant, dimA(-), encodes a bZIP/bRLZ transcription factor, which is required for every DIF-1 response investigated. Furthermore, the dimA(-) mutant shows strikingly similar developmental defect...
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PMID: 14729573
PDF is available here.
PDF is available here.
Monomeric and dimeric bZIP transcription factor GCN4 bind at the same rate to their target DNA site.
Abstract
We report the kinetics of DNA recognition by unfolded monomeric and folded dimeric derivatives of GCN4 using a 19 bp double-stranded DNA containing a palindromic CRE site. The rate of DNA binding of both monomeric and dimeric GCN4 has a bimolecular rate constant of 3-5 x 10(8) M(-1) s(-1), which is...
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PMID: 14730976
PDF is available here.
PDF is available here.
Genome Biology (5)2 2004
Abstract
We present a method for predicting protein-protein interactions mediated by the coiled-coil motif. When tested on interactions between nearly all human and yeast bZIP proteins, our method identifies 70% of strong interactions while maintaining that 92% of predictions are correct. Furthermore, cross-...
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PMID: 14759261
PDF is available here.
PDF is available here.
Nucleic Acids Research (32)17 2004
Abstract
The interaction of proteins with DNA results, in some cases, in DNA bending, and this might have functional importance. However, when the protein-induced bending of DNA is small, its measurement presents a problem. It is shown that the fluorescence resonance energy transfer between fluorophores plac...
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PMID: 15459288
PDF is available here.
PDF is available here.
Plant Cell Online (15)12 2003
Abstract
We reported previously that Arabidopsis plants expressing Pti4 constitutively express several GCC box-containing PR genes and show reduced disease symptoms compared with wild-type plants after inoculation with Pseudomonas syringae pv tomato or Erysiphe orontii. To gain insight into how genome-wide g...
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PMID: 14630974
PDF is available here.
PDF is available here.
Journal of Immunology (171)9 2003
Abstract
We demonstrate that IL-4Rs are expressed on human hepatocellular carcinoma (HCC) cells. We found that IL-4 suppresses hepatitis B surface Ag (HBsAg) mRNA and HBsAg production in the Hep3B cell line, which contains an integrated hepatitis B virus (HBV) genome and constitutively secretes HBsAg. When H...
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PMID: 14568946
PDF is available here.
PDF is available here.
Abstract
We have constructed artificial AP-1 proteins containing elements derived from yeast GCN4 and from the herpes simplex virus activator VP16. These proteins can only homodimerize but do not heterodimerize, and lacking significant homology to Jun outside the DNA-binding domain, they are largely unaffect...
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PMID: 12970741
PDF is available here.
PDF is available here.
Endocrinology (144)9 2003
Abstract
We show expression patterns of circadian clock genes in the MBH, putative suprachiasmatic nucleus (SCN), and pineal gland, which constitute the circadian pacemaker under various light schedules. Although expression patterns of clock genes were different between long and short photoperiod in the SCN...
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PMID: 12933643
PDF is available here.
PDF is available here.
Abstract
The sequence-specific inhibition of essential protein-DNA contacts in the promoter of a gene is a central issue for the regulation of gene expression by chemical methods. Hairpin polyamides have been shown to inhibit protein-DNA complexes in some but not all cases. For example, polyamides co-occupy...
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PMID: 12848553
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (278)29 2003
Abstract
We have identified the adenovirus E4 promoter-binding protein/nuclear factor regulated by IL-3 (E4bp4) as a PTH-induced primary gene in osteoblasts. E4BP4 is a basic leucine zipper (bZIP) transcription factor that represses or activates transcription in non-osteoblastic cells. We report here that PT...
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PMID: 12743120
PDF is available here.
PDF is available here.
Abstract
We have used protein arrays to test 492 pairings of a nearly complete set of coiled-coil strands from human basic-region leucine zipper (bZIP) transcription factors. We find considerable partnering selectivity despite the bZIPs' homologous sequences. The interaction data are of high quality, as asse...
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PMID: 12805554
PDF is available here.
PDF is available here.
Development Genes and Evolution (213)5-6 2003
Abstract
Many kinds of transcription factors and regulators play key roles in a variety of developmental processes. In the present survey, genes encoding proteins with conserved HMG-box, bZip domains, and some types of zinc finger motifs were surveyed in the completely sequenced genome of Ciona intestinalis....
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PMID: 12743819
PDF is available here.
PDF is available here.
Nature Biotechnology (21)5 2003
Abstract
We describe a bimolecular fluorescence complementation (BiFC) approach for the simultaneous visualization of multiple protein interactions in the same cell. This approach is based on complementation between fragments of fluorescent proteins with different spectral characteristics. We have identified...
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PMID: 12692560
PDF is available here.
PDF is available here.
Biopolymers (69)1 2003
Abstract
We have been interested in using bZIP peptides as convenient models with which to study the role of asymmetric phosphate neutralization in DNA bending. DNA bending experiments have yielded discordant results for bZIP peptides studied by electrophoretic- vs solution-based assays. We review the histor...
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PMID: 12717726
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (278)12 2003
Abstract
The structure of a protein defines its biochemical properties, but the impact of intracellular location and environment on protein structure remains poorly defined. CCAAT/enhancer-binding protein alpha (C/EBPalpha) is a master regulator of transcription and cellular proliferation that concentrates a...
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PMID: 12531886
PDF is available here.
PDF is available here.
Abstract
We show that a CLK-CYC-activated gene, vrille (vri), encodes a repressor of Clk transcription, thereby identifying vri as a key negative component of the Clk feedback loop in Drosophila's circadian oscillator. The blue light photoreceptor encoding cryptochrome (cry) gene is also a target for VRI rep...
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PMID: 12546820
PDF is available here.
PDF is available here.
Acta Genetica Sinica (30)1 2003
Abstract
mRNA differential display with G-box family primer was used to analyze the differential expression of genes of the photoperiod-temperature sensitive genic male sterile(PTSGMS) line of wheat, BAU3338, between the sterile and fertile conditions. The result indicated that gene expression was significan...
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PMID: 12812077
PDF is available here.
PDF is available here.
Plant Cell Online (15)1 2003
Abstract
We show that HvABI5 and HvVP1 are necessary for the ABA induction of gene expression but have no effect on another hormone-regulated process, the gibberellin-induced and ABA-suppressed expression of alpha-amylase. Our work indicates that although other typical plant bZIP transcription factors may bi...
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PMID: 12509536
PDF is available here.
PDF is available here.
Journal of Virology (77)2 2003
Abstract
We report that K-bZIP modulates K-Rta function. We show that K-bZIP directly interacts with K-Rta in vivo and in vitro. This association is specific, requiring the basic domain (amino acids 122 to 189) of K-bZIP and a specific region (amino acids 499 to 550) of K-Rta, and can be detected with K-bZIP...
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PMID: 12502859
PDF is available here.
PDF is available here.
Abstract
We call the O2 gene family. Members of the family share a highly conserved bZIP DNA binding domain and several other motifs which define important functional features. The O2 family was enriched by the identification of 25 new putative angiosperm O2 homologous genes in EST databases and in the rice...
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PMID: 12569427
PDF is available here.
PDF is available here.
Journal of Virology (76)24 2002
Abstract
We report in this study the characterization of a novel viral protein encoded by the complementary strand of the HTLV-1 RNA genome. This protein, designated HBZ (for HTLV-1 bZIP factor), contains a N-terminal transcriptional activation domain and a leucine zipper motif in its C terminus. We show her...
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PMID: 12438606
PDF is available here.
PDF is available here.
Abstract
E4BP4, a mammalian basic leucine zipper (bZIP) transcription factor, was first identified through its ability to bind and repress viral promoter sequences. Subsequently, E4BP4 and homologues in other species have been implicated in a diverse range of processes including commitment to cell survival v...
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PMID: 12386933
PDF is available here.
PDF is available here.
Abstract
We have cloned a gene (seb1) from T. atroviride which encodes a C2H2 zinc-finger protein that is 62 (64)% identical to S. cerevisiae Msn2p (Msn4p) in the zinc-finger region, and almost identical to the G-box binding protein from Haematonectria haematococca and to polypeptides encoded by uncharacteri...
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PMID: 12395196
PDF is available here.
PDF is available here.
Plant Journal (32)2 2002
Abstract
We identified, in a yeast two-hybrid screen, two NIM1/NPR1 interacting proteins, TGA2 and TGA5, which belong to the basic region, leucine zipper (bZIP) family of transcription factors. Both TGA2 and TGA5 strongly interact with NIM1/NPR1 in yeast and in vitro, and recognize the as-1 cis element found...
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PMID: 12383081
PDF is available here.
PDF is available here.