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Heterogeneous-Nuclear Ribonucleoprotein K (0):
Recent article
Heterogeneous-Nuclear Ribonucleoprotein K
Abstract
We herein show that TP is highly induced by serum deprivation in NPC cells, and that this is due to an increase in the half-life of the TP mRNA, as shown by nuclear run-on and actinomycin D assays. We further show that the CU-rich element of the TP mRNA directly interacts with hnRNP K, as demonstrat...
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PMID: 19330019
PDF is available here.
PDF is available here.
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Abstract
We identified cancer-specific trans-regulatory proteins that interact with the hTERT promoter, using the promoter magnetic precipitation assay coupled with mass spectrometry. The identified proteins include MutS homolog 2 (MSH2), heterogeneous nuclear ribonucleoprotein (hnRNP) D, hnRNP K and grainyh...
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PMID: 19015635
PDF is available here.
PDF is available here.
Journal of General Virology (89)Pt 10 2008
Abstract
These results suggest that hnRNP K interacts with the EV71 5' UTR and participates in virus replication....
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PMID: 18796723
PDF is available here.
PDF is available here.
Abstract
We link these two sets of enigmatic results. We find that antisense transcripts are the target for agRNAs that activate or repress expression of progesterone receptor (PR). agRNAs recruit Argonaute proteins to PR antisense transcripts and shift localization of the heterogeneous nuclear ribonucleopro...
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PMID: 18604220
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (283)26 2008
Abstract
We have set up an inducible human erythroid cell system. We show that, during induction, K562 cells exhibit changes in morphology and protein expression that are characteristic for terminal erythroid maturation: nuclear exclusion, expression of endogenous human r15-LOX regulated by hnRNP K and hnRNP...
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PMID: 18441016
PDF is available here.
PDF is available here.
Clinical Cancer Research (14)12 2008
Abstract
Cytoplasmic hnRNP K and high TP are associated with shorter OS and distant metastasis-free survival in NPC patients. In vitro experiments suggest that NPC tumors with high TP expression may be sensitive to 5'-DFUR treatment. Cytoplasmic hnRNP K and high TP may be potential prognostic and therapeutic...
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PMID: 18559600
PDF is available here.
PDF is available here.
FEBS Letters (582)12 2008
Abstract
We observed that hnRNP K was markedly arginine methylated in response to UV radiation. Furthermore, arginine methylation of hnRNP K enhanced its affinity with p53. Inhibition of methylation in hnRNP K attenuated the recruitment of p53 to p21 promoter, and reduced p53 transcriptional activity. These...
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PMID: 18472002
PDF is available here.
PDF is available here.
Cellular Signalling (20)5 2008
Abstract
We have previously shown that Ang II rapidly increases VEGF protein synthesis in proximal tubular epithelial (MCT) cells by augmenting mRNA translation, which is partly dependent on activation and binding of hnRNP K to 3' untranslated region (UTR) of VEGF mRNA. Regulation of hnRNP K activation by PK...
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PMID: 18295448
PDF is available here.
PDF is available here.
Abstract
We performed an extensive proteomic analysis of the Dahl model of salt-sensitive hypertension. The consomic SS-13(BN) rat, genetically similar to the Dahl salt-sensitive rat, while exhibiting a significant amelioration of salt-induced hypertension, was used as a control. Proteomic analysis, using di...
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PMID: 18316652
PDF is available here.
PDF is available here.
Brain Research (1189) 2008
Abstract
We performed a co-immunoprecipitation assay on homogenates of postnatal and developing rat cerebral cortex. We found that all three NF mRNAs indeed associated endogenously with these RNPs and that the degree of this association changed during postnatal development, a period when NF expression is und...
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PMID: 18054780
PDF is available here.
PDF is available here.
Abstract
We produced constructs in which the yeast Gal4 DNA-binding domain was fused to various hnRNP K fragments in one-hybrid mammalian cells. Our results reveal that the K nuclear shuttling (KNS) domain, a well-known signal for nuclear import and export, is also responsible for the transactivation activit...
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PMID: 18346927
PDF is available here.
PDF is available here.
Abstract
We show that heterogeneous nuclear ribonucleoprotein K (hnRNP K), a member of the K homology domain-containing proteins, is expressed in Xenopus immature oocytes, unfertilized eggs, and early embryos. Fertilization or egg activation treatment involving upregulation of the egg tyrosine kinase Src pro...
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PMID: 18042150
PDF is available here.
PDF is available here.
Proteomics (8)2 2008
Abstract
Our results illustrate the large rearrangement of the proteome during the differentiation of human myoblasts and provide evidence for new partners involved in this complex process....
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PMID: 18203276
PDF is available here.
PDF is available here.
Abstract
We identified the heterogeneous nuclear ribonucleoprotein K (hnRNP K) and poly(C) binding protein 1 (PCBP1) bound with the C-rich region in gastrin mRNA 3' untranslated region. Nucleolin bound with the AGCCCU motif and interacted with hnRNP K were also demonstrated. Under EGF treatment, we observed...
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PMID: 17928403
PDF is available here.
PDF is available here.
Abstract
We were able to identify proteins enriched in cytoplasmic membrane fractions containing viral RNA synthetic complexes following infection with Sindbis virus. Our studies demonstrated the following: (i) the host protein hnRNP K is enriched in cytoplasmic membrane fractions following Sindbis virus inf...
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PMID: 17561226
PDF is available here.
PDF is available here.
Abstract
Thirteen human gastric carcinoma-associated antigens were found by SERPA. The results provide foundation for screening molecular biomarkers for the diagnosis, therapy, prognostic evaluation, and prevention of human gastric carcinoma....
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PMID: 17927877
PDF is available here.
PDF is available here.
Abstract
Abstract
ANG II rapidly increases VEGF synthesis in proximal tubular epithelial cells through mRNA translation. The role of heterogeneous nuclear ribonucleoprotein K (hnRNP K) in ANG II regulation of VEGF mRNA translation initiation was examined. ANG II activated hnRNP K as judged by binding to poly(C)- and...
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PMID: 17581920
PDF is available here.
PDF is available here.
Abstract
We describe RNA Affinity in Tandem (RAT), an original, entirely RNA tag-based method for affinity purification of endogenously assembled RNP complexes. We demonstrate the general utility of RAT by isolating RNPs assembled in vivo on ncRNAs transcribed by RNA polymerase II or III. Using RAT in conjun...
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PMID: 17456562
PDF is available here.
PDF is available here.
Abstract
Abstract
We address the question how hnRNP K interacts with and activates c-Src. We define the proline residues in hnRNP K in the proline-rich motifs P2 (amino acids [aa] 285 to 297) and P3 (aa 303 to 318), which are necessary and sufficient for the specific activation of c-Src, and we dissect the amino acid...
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PMID: 17178840
PDF is available here.
PDF is available here.
Biochemical Journal (399)2 2006
Abstract
We have shown that DAZ cannot bind simultaneously to DAZAP1 and PABP, and suggest that the phosphorylation-induced dissociation of DAZ and DAZAP1 may allow the former to stimulate translation by interacting with PABP....
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PMID: 16848763
PDF is available here.
PDF is available here.
Cellular Signalling (18)10 2006
Abstract
We describe a newly identified regulatory subunit (PITK; Phosphatase Interactor Targeting K protein) that specifically targets the catalytic subunit of PP1 to nuclear foci to selectively bind and dephosphorylate the transcriptional regulator heterogeneous nuclear ribonucleoprotein K (hnRNP K) at a r...
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PMID: 16564677
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (281)35 2006
Heterogeneous nuclear ribonucleoprotein K modulates angiotensinogen gene expression in kidney cells.
Abstract
The present studies aimed to identify the 70-kDa nuclear protein that binds to an insulin-responsive element in the rat angiotensinogen gene promoter and to define its action on angiotensinogen gene expression. Nuclear proteins were isolated from rat kidney proximal tubular cells and subjected to tw...
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PMID: 16837467
PDF is available here.
PDF is available here.
Journal of Molecular Biology (361)3 2006
Abstract
We investigate which of the three KH domains of hnRNP E1 and hnRNP K mediate the DICE interaction. Using RNA-binding assays, we demonstrate DICE-binding of the KH domains 1 and 3 of hnRNP E1, and KH domain 3 of hnRNP K. Furthermore, with RNA-binding assays, NMR experiments and in vitro translation s...
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PMID: 16854432
PDF is available here.
PDF is available here.
Abstract
We have extended this earlier work and report here the spatially- and developmentally-regulated expression of hnRNP K mRNA during development of the rat nervous system. In the central nervous system, hnRNP K mRNA expression gradually decreases during development until it is restricted to a very limi...
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PMID: 16488668
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (281)22 2006
Abstract
We identify a direct interaction between N-WASP and hnRNPK. We show that this interaction is mediated by the N-terminal WH1 domain of N-WASP and the segment of hnRNPK containing its K interaction (KI) domain. Furthermore, these two proteins are co-localized at the cell periphery in the spreading ini...
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PMID: 16574661
PDF is available here.
PDF is available here.
EMBO Journal (25)9 2006
Abstract
PDGF is a potent chemotactic mitogen and a strong inductor of fibroblast motility. In Swiss 3T3 fibroblasts, exposure to PDGF but not EGF or IGF-1 causes a rapid loss of actin stress fibers (SFs) and focal adhesions (FAs), which is followed by the development of retractile dendritic protrusions and...
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PMID: 16619033
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (281)16 2006
Abstract
Arginine methylation is a post-translational modification found in many RNA-binding proteins. Heterogeneous nuclear ribonucleoprotein K (hnRNP K) from HeLa cells was shown, by mass spectrometry and Edman degradation, to contain asymmetric N(G),N(G)-dimethylarginine at five positions in its amino acid...
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PMID: 16492668
PDF is available here.
PDF is available here.
FEBS Letters (580)7 2006
Abstract
We conclude that hnRNP K protein may be a mediator of mitochondrial response to insulin....
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PMID: 16519889
PDF is available here.
PDF is available here.
Abstract
We amplified and sequenced K protein mRNA that was isolated from colorectal cancers as well as from normal tissues surrounding the tumours. Sequencing revealed a single G-to-A base substitution at position 274 that was found in tumours and surrounding mucosa, but not in individuals that had no color...
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PMID: 16404425
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1764)2 2006
Abstract
We used an integrated approach of mass spectrometry and computer analysis to explore patterns of K protein phosphorylation. We found that in vitro a single kinase can phosphorylate K protein on multiple sites spanning the entire length of the protein, including residues contained within the RNA/DNA-...
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PMID: 16448870
PDF is available here.
PDF is available here.
Acta Biochimica Polonica (53)1 2006
Abstract
We used a SAGE-based assay to profile RNAs associated with hnRNP K protein in rat mitochondria. RNA was isolated from mitoplasts obtained from highly purified and RNase-treated mitochondria. Total RNA and RNA associated with hnRNP K protein were then used as input material for generating two SAGE li...
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PMID: 16496041
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1759)11-12 2006
Abstract
Nutrient regulation of glucose-6-phosphate dehydrogenase (G6PD) expression occurs through changes in the rate of splicing of G6PD pre-mRNA. This posttranscriptional mechanism accounts for the 12- to 15-fold increase in G6PD expression in livers of mice that were starved and then refed a high-carbohyd...
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PMID: 17095106
PDF is available here.
PDF is available here.
Abstract
We identify heterogeneous nuclear ribonucleoprotein K (hnRNP K) as being rapidly induced by DNA damage in a manner that requires the DNA-damage signaling kinases ATM or ATR. Induction of hnRNP K ensues through the inhibition of its ubiquitin-dependent proteasomal degradation mediated by the ubiquiti...
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PMID: 16360036
PDF is available here.
PDF is available here.
Biochemistry (Washington) (44)50 2005
Abstract
The nuclease hypersensitive element (NHE) III(I) of the c-myc promoter regulates the expression of oncogene c-myc and hence is an important anti-cancer target. Paranemic secondary structure formation within the promoter has been implicated in mechanistic regulation models. Here, it is shown that two...
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PMID: 16342935
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (280)46 2005
Abstract
We have determined the crystal structure, at 1.7-A resolution, of PCBP2 KH1 in complex with a 7-nucleotide DNA sequence (5'-AACCCTA-3') corresponding to one repeat of the human C-rich strand telomeric DNA. The protein-DNA interaction is mediated by the combination of several stabilizing forces inclu...
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PMID: 16186123
PDF is available here.
PDF is available here.
Abstract
We show that heterogeneous nuclear ribonucleoprotein complex K (hnRNP-K) protein levels increase in SMC with response to serum stimulation in vitro, in the aortas from an animal model of atherosclerosis, and in occluded human vein segments. hnRNP-K is a multi-functional protein that has been studied...
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PMID: 15962305
PDF is available here.
PDF is available here.
Abstract
We identified eIF4E as a c-Myc target, little else is known about its transcriptional regulation. Previously, we described an element at position -25 (TTACCCCCCCTT) that was critical for eIF4E promoter function. Here we report that this sequence (named 4EBE, for eIF4E basal element) functions as a b...
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PMID: 16024782
PDF is available here.
PDF is available here.
Abstract
We report the beginning of a systematic study of each of the alphaCP KH domains, with the cloning and expression of alphaCP1-KH2 and alphaCP1-KH3. We report the ability of alphaCP1-KH3, but not alphaCP1-KH2, to bind the target AR mRNA sequence using an RNA electrophoretic mobility gel shift assay. W...
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PMID: 15756586
PDF is available here.
PDF is available here.
Abstract
We determined crystal structures of hnRNP K KH3 domain complexed with 15-mer and 6-mer (CTC(4)) ssDNAs at 2.4 and 1.8 A resolution, respectively, and show that the KH3 domain binds specifically to both TCCC and CCCC sequences. In parallel, we used NMR to compare the binding affinity and mode of inte...
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PMID: 16004877
PDF is available here.
PDF is available here.
Immunology Letters (98)2 2005
Abstract
We analyzed proteins of Ramos B cell line that are altered upon BCR activation with anti-IgM antibody, revealing that a certain hnRNP K isoform is up-regulated in response to anti-IgM treatment. We also showed that hnRNP K is tyrosine-phosphorylated after BCR ligation. HnRNP K lacking the SH3BD is s...
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PMID: 15860232
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (280)13 2005
Abstract
We show that Hu induces p21 expression, cell cycle arrest, and neuronal differentiation in mouse neuroblastoma N1E-115 cells. p21 expression is also up-regulated during Me2SO-induced differentiation in N1E-115 cells and is controlled by post-transcriptional mechanisms through its 3'-UTR. To investig...
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PMID: 15671036
PDF is available here.
PDF is available here.
Autoimmunity (38)1 2005
Abstract
Heterogeneous nuclear ribonucleoproteins (hnRNPs) are among the most abundant proteins in the eukaryotic cell nucleus and play a direct role in several aspects of the RNA life including splicing, export of the mature RNAs and translation. To date, approximately 30 proteins have been identified. A gr...
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PMID: 15804702
PDF is available here.
PDF is available here.
Biochemical Journal (385)Pt 2 2005
Abstract
We identify hnRNP K as a novel repressor of osteocalcin gene transcription. Overexpression of hnRNP K lowers the expression of osteocalcin mRNA by 5-fold. Furthermore, luciferase reporter assays demonstrate that overexpression of hnRNP K represses osteocalcin transcription from a CT (cytosine/thymid...
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PMID: 15361071
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (279)52 2004
Abstract
These results suggest the insulin stimulates translation of ucp2 mRNA in a process that involves K protein....
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PMID: 15485813
PDF is available here.
PDF is available here.
FEBS Letters (577)1-2 2004
Abstract
We used full-length hnRNP K, its fragments and the yeast ortholog as baits in the yeast three-hybrid system. The results demonstrate that in this heterologous in vivo system, the three KH domains bind RNA synergistically and that a single KH domain, in comparison, binds RNA weakly....
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PMID: 15527774
PDF is available here.
PDF is available here.
Journal of Molecular Biology (342)4 2004
Abstract
We used a serial analysis of gene expression (SAGE)-based strategy, yeast three-hybrid screen, RNA pull-down assays and computational analysis to characterize K protein-associated RNAs. We demonstrate that K protein interacts with many sense and antisense nuclear and mitochondrial transcripts throug...
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PMID: 15351640
PDF is available here.
PDF is available here.
Biology of the Cell (96)6 2004
Abstract
Studies on the post-transcriptional regulation of gene expression in haematopoietic cells have uncovered that a subfamily of heterogeneous nuclear ribonucleoproteins (hnRNPs) is involved in cytoplasmic gene regulation. HnRNP K and hnRNP E1/E2 share a common structural motif, the hnRNP K homology (KH...
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PMID: 15384226
PDF is available here.
PDF is available here.
Abstract
Since its original identification as a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex, K protein has been found not only in the nucleus but also in the cytoplasm and mitochondria and is implicated in chromatin remodeling, transcription, splicing and translation processes. K...
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PMID: 15170860
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (279)19 2004
Abstract
We previously demonstrated that the 3'-untranslated region of BCL-2 mRNA contains an ARE that accounts for rapid BCL-2 down-regulation in response to apoptotic stimuli. We also demonstrated that the BCL-2 ARE core interacts with a number of ARE-binding proteins, one of which is AU-rich factor 1/hete...
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PMID: 14769789
PDF is available here.
PDF is available here.
Abstract
We have identified several RNA binding proteins with translation regulatory activity whose expression is markedly activated in the blast crisis of chronic myelogenous leukemia, which represents the most malignant disease stage. Perturbation of the activity of some RNA binding proteins suppresses the...
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PMID: 15094772
PDF is available here.
PDF is available here.
Acta Crystallographica Section D (60)Pt 4 2004
Abstract
hnRNP K is one of the major proteins found in hnRNP particles which are ribonucleoprotein complexes containing proteins and pre-mRNA. hnRNP K contains hnRNP K homology (KH) domains which bind both CT-rich single-stranded DNA (ssDNA) and CU-rich ssRNA. Co-crystallization of the third KH domain of hum...
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PMID: 15039586
PDF is available here.
PDF is available here.
Abstract
Our results suggest that the exclusion of proteins by the ER quality control system, crosstalk between the EGF- and INF-induced signalling pathways and the regulation of INF-inducible genes are all altered in the prostate cancer cells. The combination of upregulated activity in the growth-promoting...
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PMID: 14647428
PDF is available here.
PDF is available here.
Nucleic Acids Research (32)18 2004
Abstract
We suggest that ORF57 and hnRNP K interaction may modulate ORF57-mediated regulation of viral gene expression. Herpesviral ORF57 (Rhadinovirus) and ICP27 (Simplexvirus) proteins both interact with hnRNP K and CK2 implying that adaptation of the ancestral hnRNP K and CK2 to associate with viral regul...
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PMID: 15486205
PDF is available here.
PDF is available here.
Abstract
We used serum-treated hepatocyte culture, liver after partial hepatectomy and hepatic neoplasms as systems to compare expression, subcellular distribution and tyrosine phosphorylation of K protein in quiescent and dividing cells. The results show that expression of K protein mRNA was increased in st...
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PMID: 14562022
PDF is available here.
PDF is available here.
Abstract
We show that members of the poly (rC) binding protein family, poly (rC) binding protein 1 (PCBP1), poly (rC) binding protein 2 (PCBP2) and hnRNPK were able to activate the IRES in vitro up to threefold when added in combination with upstream of N-ras and unr-interacting protein. The interactions of...
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PMID: 12970749
PDF is available here.
PDF is available here.
Abstract
We used chromatin immunoprecipitation (ChIP) assays to explore K protein interactions with serum-inducible, constitutively expressed and untranscribed gene loci in vivo. In the rat HTC-IR hepatoma cell line, serum treatment induced transient increases in the mRNA levels of two immediate-early genes,...
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PMID: 12853611
PDF is available here.
PDF is available here.
Journal of Virology (77)7 2003
CK2 protein kinase is stimulated and redistributed by functional herpes simplex virus ICP27 protein.
Abstract
We show here that HSV-1 infection stimulates CK2 activity. CK2 stimulation occurs at early times after infection and correlates with redistribution of the holoenzyme from the nucleus to the cytoplasm. Both CK2 stimulation and redistribution require expression and cytoplasmic accumulation of ICP27. I...
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PMID: 12634389
PDF is available here.
PDF is available here.
Nucleic Acids Research (31)5 2003
Abstract
We described an unusual factor called SRC pyrimidine-binding protein (SPy) that could bind to two of these TC tracts in their double-stranded form, but was also capable of interacting with higher affinity to all three pyrimidine tracts in their single-stranded form. Mutations in the TC tracts, which...
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PMID: 12595559
PDF is available here.
PDF is available here.