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Origin Recognition Complex (0):
Recent article
Origin Recognition Complex
Abstract
We show that the edges of the rDNA array are exceptionally susceptible to meiotic DSBs, revealing an inherent heterogeneity in the rDNA array. We find that this localized DSB susceptibility necessitates a border-specific protection system consisting of the meiotic ATPase Pch2 and the origin recognit...
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PMID: 21822291
PDF is available here.
PDF is available here.
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Eukaryotic Cell (10)9 2011
Abstract
We highlight how this diversification has been facilitated by gene duplications and has contributed to adaptations in lifestyle....
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PMID: 21764908
PDF is available here.
PDF is available here.
Abstract
We use nucleosomes methylated on DNA and on histone H3 in an affinity assay, in conjunction with a SILAC-based proteomic analysis, to identify "crosstalk" between these two distinct classes of modification. Our analysis reveals proteins whose binding to nucleosomes is regulated by methylation of CpG...
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PMID: 21029866
PDF is available here.
PDF is available here.
Abstract
We performed in vitro and in vivo characterization of the physical interaction between the archaeon Sulfolobus solfataricus Y-family DNA polymerase (SsoPolY) and three eukaryote-like Orc1/Cdc6 proteins (SsoCdc6-1, SsoCdc6-2, and SsoCdc6-3). The effect of SsoCdc6-2 was the strongest, and the three Ss...
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PMID: 20457125
PDF is available here.
PDF is available here.
British Poultry Science (51)1 2010
Abstract
1. The objectives of this study were to isolate Salmonella spp. by conventional culture technique from ground turkey samples, to determine the seasonal distribution of Salmonella spp., to verify the isolates by PCR using primers based on oriC gene sequence, and to determine the antibiotic susceptibi...
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PMID: 20390570
PDF is available here.
PDF is available here.
Genome Research (20)2 2010
Abstract
We investigated the genome-wide distribution of ORC in Drosophila and found that ORC localizes to specific chromosomal locations in the absence of any discernible simple motif. Although no clear sequence motif emerged, we were able to use machine learning approaches to accurately discriminate betwee...
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PMID: 19996087
PDF is available here.
PDF is available here.
Abstract
We discuss the roles of DnaA, ORC, and Cdc6 in replication initiation and its regulation. We also summarize the functional features of the AAA+ domains of these proteins, and the functional divergence of ORC in chromosomal dynamics....
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PMID: 20130679
PDF is available here.
PDF is available here.
Abstract
We found that ORC physically interacts throughout the internal regions of HMR as well as with silencers. This interaction depended on the presence of Sir proteins and, in part, on the HMR-I silencer. ORC remained associated with the internal regions of HMR even when these regions were recombinationa...
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PMID: 19948882
PDF is available here.
PDF is available here.
Cell Cycle (8)22 2009
Abstract
Molecular Microbiology (74)2 2009
Abstract
We investigated the role of YabA in regulating replication initiation and the activity of DnaA as a transcription factor. We found that YabA function is mainly limited to replication initiation at oriC. Loss of YabA did not significantly alter expression of genes controlled by DnaA during exponentia...
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PMID: 19737352
PDF is available here.
PDF is available here.
Abstract
We demonstrate that depletion of either of two essential licensing factors, Cdc6 or Cdt1, in normal human fibroblasts induces a G(1) arrest accompanied by inhibition of cyclin E/Cdk2 activity and hypophosphorylation of Rb. The Cdk2 inhibition is attributed to a reduction in the essential activating...
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PMID: 19440053
PDF is available here.
PDF is available here.
Abstract
Abstract
Abstract
Abstract
Abstract
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Abstract
We report a new role for the Orc1 protein, a subunit of the origin recognition complex (ORC) that is a key component of the DNA replication licensing machinery, in controlling centriole and centrosome copy number in human cells, independent of its role in DNA replication. Cyclin A promotes Orc1 loca...
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PMID: 19197067
PDF is available here.
PDF is available here.
Abstract
We constructed a series of yeast strains expressing phospho-mimetic mutants of Orc2p or Orc6p and found that expression of a Ser-188 mutant of Orc2p (Orc2-5Dp) delays G1-S transition and S phase progression and causes the accumulation of cells with 2C DNA content. Using antibody that specifically re...
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PMID: 19068484
PDF is available here.
PDF is available here.
Abstract
Abstract
Accurate and timely duplication of chromosomal DNA requires that replication be coordinated with processes that ensure genome integrity. Significant advances in determining how the earliest steps in DNA replication are affected by DNA damage have highlighted some of the mechanisms to establish that...
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PMID: 19482602
PDF is available here.
PDF is available here.
Genome Biology (10)3 2009
Abstract
Origin recognition complex (ORC) proteins were first discovered as a six-subunit assemblage in budding yeast that promotes the initiation of DNA replication. Orc1-5 appear to be present in all eukaryotes, and include both AAA+ and winged-helix motifs. A sixth protein, Orc6, shows no structural simil...
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PMID: 19344485
PDF is available here.
PDF is available here.
Abstract
We describe a transgenic Drosophila model which enables dynamic imaging of a green fluorescent protein (GFP)-tagged Drosophila melanogaster ORC subunit, DmOrc2-GFP. It is functional in genetic complementation, expressed at physiological levels, and participates quantitatively in complex formation. T...
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PMID: 18955499
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (283)49 2008
Abstract
We report a new function for ORC in mediating histone methylation. Using the yeast two-hybrid system, we identify a physical interaction between Orc2p and Spp1p, a member of the Set1 complex, and we demonstrate the interaction between the endogenous ORC and Spp1p by co-immunoprecipitation from yeast...
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PMID: 18845545
PDF is available here.
PDF is available here.
EMBO Journal (27)22 2008
Abstract
We present evidence that ORC recruitment to OriP and DNA replication function depends on RGG-like motifs, referred to as LR1 and LR2, in the EBNA1 amino-terminal domain. Moreover, we show that LR1 and LR2 recruitment of ORC is RNA dependent. HMGA1a, which can functionally substitute for LR1 and LR2...
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PMID: 18946490
PDF is available here.
PDF is available here.
Abstract
We show that Skp2 depletion in melanoma cells resulted in a tetraploid cell cycle arrest. Surprisingly, co-knockdown of p27(Kip1) or cyclin E1 failed to prevent the tetraploid arrest induced by Skp2 knockdown. Enhanced Aurora A phosphorylation and repression of G2/M regulators cyclin B1, cyclin-depe...
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PMID: 18716061
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (283)44 2008
Abstract
We describe the interactions of highly purified Schizosaccharomyces pombe pre-RC components, SpORC, SpCdc18, and SpCdt1, with each other and with ars1 origin DNA. We show that SpORC binds DNA in at least two steps. The first step likely involves electrostatic interactions between the AT-hook motifs...
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PMID: 18723846
PDF is available here.
PDF is available here.
Abstract
We report for the first time, the cloning of ORC1 from L. major, and the analysis of its expression in L. major promastigotes. In human cells ORC1 levels have been found to be upregulated in G1 and subsequently degraded, thus playing a role in controlling replication initiation. We examine the subce...
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PMID: 18680728
PDF is available here.
PDF is available here.
Microbiology (154)Pt 9 2008
Abstract
The genome of Mycoplasma gallisepticum strain R(low) has been sequenced completely, but subsequent genetic studies have been limited by the lack of a replicable vector system. In this study, replicable plasmids were constructed for M. gallisepticum and Mycoplasma imitans using the oriC region upstre...
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PMID: 18757791
PDF is available here.
PDF is available here.
Abstract
We present evidence for site-specific DNA replication origins in Xenopus egg extracts. Using lambda DNA, we show that DNA replication origins are activated in clusters in regions that contain closely spaced adenine or thymine asymmetric tracks used as preferential initiation sites. In agreement with...
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PMID: 18573882
PDF is available here.
PDF is available here.
Nucleic Acids Research (36)16 2008
Abstract
We show here that SAS2 deletion (sas2Delta) exacerbates the temperature sensitivity of the ORC mutants orc2-1 and orc5-1. Moreover, sas2Delta and orc2-1 have a synthetic effect on cell cycle progression through S phase and initiation of DNA replication. These results suggest that SAS2 plays a positi...
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PMID: 18682530
PDF is available here.
PDF is available here.
Abstract
We have produced and analyzed a Drosophila orc1 allele to investigate the roles of ORC1 in three different modes of DNA replication during development. As expected, ORC1 is essential for mitotic replication and proliferation in brains and imaginal discs, as well as for gene amplification in ovarian...
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PMID: 18711130
PDF is available here.
PDF is available here.
Biochemistry (Washington) (47)33 2008
Abstract
We have investigated the effect of DNA-binding, AAA+ protein human ORC4 on single-stranded origin DNA or various oligonucleotides. Here we demonstrate that human ORC4 stimulated formation of inter- and intramolecular T.A.T triplexes and created novel structures, such as homoadenine duplexes. Adenine...
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PMID: 18652488
PDF is available here.
PDF is available here.
Molecular Microbiology (69)3 2008
Abstract
We identified a putative homologue of Saccharomyces cerevisiaeorigin recognition complex subunit 5 in P. falciparum (PfORC5). PfORC5 forms distinct nuclear foci colocalized with the replication foci marker proliferating cell nuclear antigen (PfPCNA) and co-immunoprecipitates with PCNA during early-t...
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PMID: 18554328
PDF is available here.
PDF is available here.
Abstract
We performed a comprehensive linker scan analysis of ARS318. Unexpectedly, this replicator depends on a 9/11-bp match to the ACS that positions the ORC binding site only 6 bp away from an Abf1p binding site. Although a largely inactive replicator on the chromosome, ARS318 becomes active if the nearb...
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PMID: 18573888
PDF is available here.
PDF is available here.
Biochemical Journal (413)3 2008
Abstract
We mutated the Orc1p sensor 1 and sensor 2 regions, which are important for ATPase activity in AAA(+) proteins. Plasmid-shuffling analysis revealed that Asn(600), Arg(694) and Arg(704) are essential for the function of Orc1p. In yeast cells, overexpression of Orc1R694Ep inhibited growth, caused inef...
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PMID: 18393942
PDF is available here.
PDF is available here.
Molecular Cell (31)2 2008
Abstract
We characterize MCM9, a member of the MCM2-8 family. We demonstrate that MCM9 binds to chromatin in an ORC-dependent manner and is required for the recruitment of the MCM2-7 helicase onto chromatin. Its depletion leads to a block in pre-RC assembly, as well as DNA replication inhibition. We show tha...
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PMID: 18657502
PDF is available here.
PDF is available here.
Abstract
We exploited the Drosophila Amplicon in Follicle Cells, DAFC-62D, to identify a new metazoan amplification origin, ori62. In addition to the origin, DAFC-62D contains two other developmental stage-specific binding regions for the Origin Recognition Complex (ORC) and the replicative helicase MCM2-7....
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PMID: 18621687
PDF is available here.
PDF is available here.
Abstract
Abstract
Journal of Cell Science (121)Pt 12 2008
Abstract
We identified, in P. falciparum, a novel telomere-associated protein that displays homology with the origin-of-recognition-complex 1 protein Orc1. Antibodies raised against this P. falciparum protein localized to telomeric clusters in the nuclear periphery and the nucleolus. It was found that, prior...
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PMID: 18525026
PDF is available here.
PDF is available here.
Abstract
Down-regulation of ORC1 mRNA and protein expression was observed in rat VSMCs at 24 h after transfection with the three pairs of siRNA targeting ORC1 gene and this reduction persisted at least 7 days post-transfection. Down-regulation of ORC1 mRNA (60%) and protein (80%) expression was observed at 7...
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PMID: 18499104
PDF is available here.
PDF is available here.
Abstract
We investigated the role of fad24 in the process. First, the expression of fad24 was examined in pre-confluent and post-confluent 3T3-L1 preadipocytes, NIH-3T3 fibroblasts, and C2C12 myoblasts. fad24 was strongly expressed in the pre-confluent cells. The knockdown of fad24 by RNA interference impair...
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PMID: 18520036
PDF is available here.
PDF is available here.
Molecular Cell (30)2 2008
Abstract
We identified five SIR2-sensitive origins on chromosomes III and VI. Linker scan analysis of two origins indicated that they share a common organization, including an inhibitory sequence positioned 3' to the sites of origin recognition complex (ORC) binding and pre-RC assembly. This inhibitory seque...
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PMID: 18439895
PDF is available here.
PDF is available here.
Cell Cycle (7)8 2008
Abstract
We propose that Orc5 has at least two distinct functions that can be separated genetically. Taken together, our results provide further support for the idea that ORC plays multiple functions during the cell cycle....
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PMID: 18414064
PDF is available here.
PDF is available here.
Genes & Development (22)7 2008
Abstract
We show that topo II couples DNA replication termination with the clearing of replication complexes for resetting replicons at mitosis. Topo II inhibition impairs completion of DNA replication, accounting for replication protein A (RPA) stabilization onto ssDNA. Topo II inhibition does not affect th...
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PMID: 18381889
PDF is available here.
PDF is available here.
Journal of Biochemistry (143)4 2008
Abstract
We constructed a heat-inducible degron mutant for each ORC subunit. As well as each targeted subunit, other subunits of ORC were also rapidly degraded under non-permissive conditions. In the orc5 degron mutant, incubation under the non-permissive conditions caused accumulation of cells with nearly 2...
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PMID: 18211918
PDF is available here.
PDF is available here.
Abstract
We investigate the DNA-binding activities of the N-terminal AAA+ ATPase domains of these Orc1/Cdc6 proteins, including their functional interactions with the other SsoCdc6 proteins, on duplex DNA substrates derived from the origins of S. solfataricus. We showed that the ATPase domain of SsoCdc6-2 re...
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PMID: 18237540
PDF is available here.
PDF is available here.
Abstract
We discuss recent reports that present structural information on the interaction characteristics of the archaeal orthologues of the eukaryotic origin recognition complex with their cognate binding sequences. Since the archaeal replication system is postulated as a simplified version of the one in eu...
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PMID: 18293358
PDF is available here.
PDF is available here.
Abstract
We designed a group of duplex DNA substrates containing specific origin recognition boxes (ORBs) of the archaeon and identified the DNA-binding activities of different SsoCdc6 proteins. Furthermore, we showed that the DNA-protein interaction between the DNA substrate and the SsoCdc6-1 or SsoCdc6-3 s...
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PMID: 18155660
PDF is available here.
PDF is available here.
Abstract
Our experiments indicate that a family member of the high-mobility group proteins, HMGA1a, can specifically target ORC to DNA. Coimmunoprecipitations and imaging studies demonstrate that HMGA1a interacts with different ORC subunits in vitro and in vivo. This interaction occurs mainly in AT-rich hete...
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PMID: 18234858
PDF is available here.
PDF is available here.
Carcinogenesis (29)2 2008
Abstract
Cell division cycle 6 (CDC6) is an essential regulator of DNA replication in eukaryotic cells. Its best-characterized function is the assembly of prereplicative complexes at origins of replication during the G(1) phase of the cell division cycle. However, CDC6 also plays important roles in the activ...
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PMID: 18048387
PDF is available here.
PDF is available here.
Genes to Cells (13)2 2008
Abstract
We analyzed living cells growing under various conditions for the number of the replication origin (oriC) copies by flow cytometry and the foci of oriC by fluorescence microscopy. The average number of the oriC foci per cell was significantly smaller than the number of oriC copies per cell with few...
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PMID: 18233960
PDF is available here.
PDF is available here.
Molecular Microbiology (67)4 2008
Abstract
We show that the alpha dimer of HU is more active than other forms of HU in initiation of an oriC-containing plasmid because it more effectively promotes strand opening of oriC. Other results demonstrate that HU stabilizes the DnaA oligomer bound to oriC, and that the alpha subunit of HU interacts w...
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PMID: 18179598
PDF is available here.
PDF is available here.
Abstract
We identified and characterized a novel gene, fad24 (factor for adipocyte differentiation 24), induced to express at the early stages of adipocyte differentiation. Although fad24 clearly has crucial roles in adipogenesis, its precise functions remain unknown. Here we show that the knockdown of fad24...
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PMID: 18029353
PDF is available here.
PDF is available here.
Biochemical Journal (409)2 2008
Abstract
We show that a previously identified cluster of ORB (origin recognition box) sequences do indeed function as an origin of replication in vivo in the archaeon Methanothermobacter thermautotrophicus. Although the consensus sequence of ORBs in M. thermautotrophicus is somewhat conserved when compared w...
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PMID: 17956224
PDF is available here.
PDF is available here.
Abstract
We demonstrated that the P. furiosus GINS complex interacts with P. furiosus MCM. A chromatin immunoprecipitation assay revealed that the GINS complex is detected preferentially at the oriC region on Pyrococcus chromosomal DNA during the exponential growth phase but not in the stationary phase. Furt...
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PMID: 17986447
PDF is available here.
PDF is available here.
Abstract
Abstract
We characterized the interaction of Drosophila Rb, Rbf1, with ORC. Using expression of proteins in Drosophila S2 cells, we found that an N-terminal Rbf1 fragment (amino acids 1-345) is sufficient for Rbf1 association with ORC but does not bind to dE2F1. We also found that the C-terminal half of Rbf1...
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PMID: 18665226
PDF is available here.
PDF is available here.
Abstract
We investigated the DNA-binding activities of two eukaryote-like Orc1/Cdc6 proteins (SsoCdc6-1 and -2) and interactions between them on the different structural duplex DNA substrates derived from oriC1 of Sulfolobus solfataricus. The results showed that two Orc1/Cdc6 proteins stimulated mutual DNA-b...
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PMID: 17964284
PDF is available here.
PDF is available here.