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Replication Protein C (0):
Recent article
Replication Protein C
Toxicology and Applied Pharmacology (246)1-2 2010
Abstract
We have investigated the influence of TCDD on functional Rfc1 activity in rat liver. Pre-treatment of rats with TCDD significantly diminished hepatocellular Rfc1 uptake activity in a time- and dose-dependent manner. In further mechanistic studies we demonstrated that this reduction was due to TCDD-d...
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PMID: 20451541
PDF is available here.
PDF is available here.
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Abstract
Abstract
Abstract
(1) HPV16 infection rate in cases (56.07%) was higher than that in controls (31.78%) with the adjusted OR of 3.25 (95% CI: 1.74-6.08). (2) All the genotypes were tested by Hardy-Weinberg balance. (3) Compared with RFC-1 AA,RFC-1 GG had higher risk for cervical cancer with OR of 2.42 (95% CI: 1.01-5....
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PMID: 19565885
PDF is available here.
PDF is available here.
Abstract
DNA replication is a complex mechanism that functions due to the co-ordinated interplay of several dozen protein factors. In the last few years, numerous studies suggested a tight implication of DNA replication factors in several DNA transaction events that maintain the integrity of the genome. Ther...
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PMID: 19563099
PDF is available here.
PDF is available here.
Abstract
We also present two easy methods to isolate the homotrimeric human PCNA and the heterotrimeric human RP-A from an Escherichia coli expression system....
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PMID: 19563116
PDF is available here.
PDF is available here.
BMC Microbiology (9) 2009
Abstract
Three primary isolates (PT1-3) and two established cervical cancer cell lines were compared to normal keratinocytes (NK) for their ability to replicate AAV. One isolate, PT3, allowed for high levels of AAV DNA replication and virion production compared to others. In research by others, four cellular...
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PMID: 19389243
PDF is available here.
PDF is available here.
Abstract
We studied the dynamics and variability of the protein response of human cancer cells to a chemotherapy drug, camptothecin. We present a dynamic-proteomics approach that measures the levels and locations of nearly 1000 different endogenously tagged proteins in individual living cells at high tempora...
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PMID: 19023046
PDF is available here.
PDF is available here.
Clinica Chimica Acta (398)1-2 2008
Abstract
Maternal tHcy was affected by MTHFR C677T, RFC1 A80G and GCPII C1561T polymorphisms. Maternal GCPII C1561T variant was associated with neonatal MMA. Neonatal RFC1 A80G polymorphism influenced tHcy in neonates....
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PMID: 18823966
PDF is available here.
PDF is available here.
Abstract
uvsF201 was the first highly UV-sensitive repair-defective mutation isolated in Aspergillus nidulans. It showed epistasis only with postreplication repair mutations, but caused lethal interactions with many other repair-defective strains. Unexpectedly, closest homology of uvsF was found to the large...
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PMID: 18655840
PDF is available here.
PDF is available here.
Abstract
We show that cells lacking Hst3p and Hst4p, in which H3 K56 is constitutively hyperacetylated, exhibit hallmarks of spontaneous DNA damage, such as activation of the checkpoint kinase Rad53p and upregulation of DNA-damage inducible genes. Consistently, hst3 hst4 cells display synthetic lethality int...
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PMID: 18579506
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (283)30 2008
Abstract
We purified the protein from 293 cells and demonstrated that hChlR1 possesses DNA-dependent ATPase and helicase activities. This helicase translocates on single-stranded DNA in the 5' to 3' direction in the presence of ATP and, to a lesser extent, dATP. Its unwinding activity requires a 5'-singlestr...
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PMID: 18499658
PDF is available here.
PDF is available here.
Abstract
We describe evidence that PCNA can affect silencing in Saccharomyces cerevisiae by facilitating deposition of H3 K56ac onto chromosomes. We propose that PCNA participates in this process through a pathway that includes replication factor C, the chromatin assembly factor Asf1p, and the K56-specific a...
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PMID: 18558650
PDF is available here.
PDF is available here.
Abstract
We investigated preliminarily whether methylenetetrahydrofolate reductase (MTHFR) 677C/T or reduced folate carrier 1 (RFC1) 80G/A polymorphisms were associated with toxicities during maintenance chemotherapy with mercaptopurine (6MP) and methotrexate (MTX) in children with acute lymphoblastic leukemi...
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PMID: 18458567
PDF is available here.
PDF is available here.
Neuroscience (152)4 2008
Abstract
We have expressed A-FOS, an inhibitor of activator protein-1 (AP-1) DNA binding, in adult mouse striatal neurons. We observed normal behavior including locomotion and exploratory activities. Following a single injection of cocaine, locomotion increased similarly in both the A-FOS expressing and litt...
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PMID: 18355967
PDF is available here.
PDF is available here.
Molecular Microbiology (68)1 2008
Abstract
We exploit the asymmetry of S. solfataricus PCNA to create a series of circularly permuted PCNA subunit fusions, thereby covalently closing defined interfaces within the heterotrimer. Using these concatamers, we investigate the requirements for loading the clamp onto DNA and reveal that a single def...
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PMID: 18312273
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (283)14 2008
Abstract
We show that replication factor C (RFC; a heteropentameric protein complex that loads PCNA onto DNA) was also ubiquitylated in a RAD18-dependent manner in cells treated with alkylating agents or H(2)O(2). A mutant form of RFC2 with a D228A substitution (corresponding to a yeast Rfc4 mutation that re...
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PMID: 18245774
PDF is available here.
PDF is available here.
Abstract
We report studies of fission yeast Ctf18, a subunit of the RFC(Ctf18) replication factor C complex, and Chl1, a putative DNA helicase. We show that RFC(Ctf18) is essential in the absence of the Swi1-Swi3 replication fork protection complex required for the S phase stress response. Loss of Ctf18 lead...
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PMID: 18045993
PDF is available here.
PDF is available here.
Abstract
These results suggest that in the Shanxi population RFC-1 polymorphisms may play a role in NTD risk, whereas the impact of MTHFR C677T polymorphisms requires further clarification. Birth Defects Research (Part A) 2008.
(c) 2007 Wiley-Liss, Inc....
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PMID: 18022874
PDF is available here.
PDF is available here.
Chemotherapy (54)3 2008
Abstract
MTA showed potent cytotoxicity against 211H cells (IC(50), 67 nM for 48 h exposure), compared to NSCLC cell lines. Significantly higher expression of FPGS and RFC1 mRNAs in 211H cells were associated with MTA sensitivity. Simultaneous exposure of MTA and GEM was antagonistic in all cell lines tested...
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PMID: 18560222
PDF is available here.
PDF is available here.
BMC Genomics (9)1 2008
Abstract
Affymetrix microarray analysis of the relative gene expression profiles in whole E9.5 RFC1-/- vs. RFC1+/+ embryos identified 200 known genes that were differentially expressed. Major ontology groups included transcription factors (13.04%), and genes involved in transport functions (ion, lipid, carbo...
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PMID: 18400109
PDF is available here.
PDF is available here.
Abstract
The aim of the present study was to investigate the effect of polymorphisms C677T and A1298C in the methylenetetrahydrofolate reductase (MTHFR) gene, A2756G in methionine synthase reductase (MTR) gene and A80G in reduced folate carrier 1 (RFC1) gene, and plasma homocysteine (Hcy), on the maternal ri...
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PMID: 18273817
PDF is available here.
PDF is available here.
EMBO Journal (26)22 2007
Abstract
Journal of Biological Chemistry (282)45 2007
Abstract
We report that equol inhibits 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced neoplastic transformation of JB6 P+ mouse epidermal cells by targeting the MEK/ERK/p90RSK/activator protein-1 signaling pathway. TPA-induced neoplastic cell transformation was inhibited by equol, but not daidzein, at no...
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PMID: 17724030
PDF is available here.
PDF is available here.
Abstract
Defects in DNA replication fidelity lead to genomic instability. Gross chromosomal rearrangement (GCR), a type of genomic instability, is highly enhanced by various initial mutations affecting DNA replication. Frequent observations of GCRs in many cancers strongly argue the importance of maintaining...
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PMID: 17689491
PDF is available here.
PDF is available here.
Abstract
Biochemistry (Washington) (46)30 2007
Abstract
We test whether replication accessory proteins enhance the fidelity of TLS by pol eta. We first show that the single-stranded DNA binding protein RPA, the sliding clamp PCNA, and the clamp loader RFC slightly increase the processivity of yeast pol eta and its ability to recycle to new template prime...
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PMID: 17608453
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (282)31 2007
Abstract
We show that DNA ligase I also interacts with the hRad17 subunit of the hRad17-RFC cell cycle checkpoint clamp loader, and with each of the subunits of its DNA sliding clamp, the heterotrimeric hRad9-hRad1-hHus1 complex. In contrast to the inhibitory effect of RFC, hRad17-RFC stimulates joining by D...
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PMID: 17561505
PDF is available here.
PDF is available here.
Journal of Cell Science (120)Pt 15 2007
Abstract
To produce viable progeny, cells must identify the products of chromosome replication as sister chromatids, pair them together and then maintain this cohesion until chromosome segregation. It is well established that cohesin ring-like structures maintain sister chromatid cohesion, but the molecular...
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PMID: 17646671
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (282)29 2007
Abstract
We propose that this novel loader-DNA polymerase interaction allows DNA replication forks to overcome interference by various template structures, including damaged DNA and DNA-protein complexes that maintain chromosome cohesion....
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PMID: 17545166
PDF is available here.
PDF is available here.
Abstract
We examined ATR-pathway function in cell lines from three haploinsufficient contiguous gene-deletion disorders--a subset of blepharophimosis-ptosis-epicanthus inversus syndrome, Miller-Dieker lissencephaly syndrome, and Williams-Beuren syndrome--in which the deleted region encompasses ATR, RPA1, and...
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PMID: 17564965
PDF is available here.
PDF is available here.
Brain Research (1154) 2007
Abstract
We report here the transcriptional regulation of PSA-NCAM expression by subtoxic dose of NMDA in retinoic acid-differentiated SH-SY5Y cell cultures. SH-SY5Y cell cultures differentiated with retinoic acid (10 microM) were exposed to NMDA (100 microM) or to antagonist MK-801 (200 nM) prior to treatme...
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PMID: 17499225
PDF is available here.
PDF is available here.
Abstract
Replication factor C (RFC), consisting of one large subunit and four small subunits, is an important factor involved in DNA replication and repair mechanisms as well as cell proliferation. The subunit 1 of Arabidopsis RFC (AtRFC1) is a homologue of p140, the large subunit of human RFC. Three T-DNA i...
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PMID: 17556804
PDF is available here.
PDF is available here.
Journal of Virology (81)11 2007
Abstract
We fractionated adenovirus-infected crude extracts and tested them in an in vitro replication system that required the presence of the AAV-encoded Rep protein and the AAV origins of DNA replication, thus faithfully reproducing in vivo viral DNA replication. Fractions that contained replication facto...
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PMID: 17360744
PDF is available here.
PDF is available here.
FEBS Journal (274)7 2007
Abstract
We first identified the gene (rfc1) encoding the Drosophila RFC140 (dRFC140) protein and then isolated a mutant. The phenotypes suggested that the gene is essential for cell-cycle progression, and immunocytochemical studies also indicated a relation between its expression and the cell cycle. The rfc...
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PMID: 17381512
PDF is available here.
PDF is available here.
Molecular Microbiology (64)2 2007
Abstract
We describe here the biochemical properties of the three PCNAs, PCNA1, PCNA2 and PCNA3, from the hyperthermophilic archaeon, Aeropyrum pernix. PCNA2 can form a trimeric structure by itself, and it also forms heterotrimeric structures with PCNA1 and PCNA3. However, neither PCNA1 nor PCNA3 can form ho...
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PMID: 17493121
PDF is available here.
PDF is available here.
Biochemical Journal (402)3 2007
Abstract
We show a new molecular mechanism of SHP-mediated inhibition of PEPCK transcription. We also show that the CRE1 (cAMP regulatory element 1; -99 to -76 bp relative to the transcription start site) of the PEPCK promoter is also required for the inhibitory regulation by SHP. SHP repressed C/EBPalpha (C...
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PMID: 17094771
PDF is available here.
PDF is available here.
Abstract
Stereoselectivity of the human reduced folate carrier (RFC1) in Caco-2 cells was examined using methotrexate (L-amethopterin, L-MTX) and its antipode (D-amethopterin, D-MTX) as model substrates. The initial uptake rate of L-MTX into Caco-2 cells followed Michaelis-Menten kinetics with a Km value of...
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PMID: 17329909
PDF is available here.
PDF is available here.
Journal of Hypertension (25)3 2007
Abstract
X/XO did not affect HASMC proliferation, but led to enhanced planar cell surface area and protein synthesis. In addition, X/XO enhanced c-jun levels and AP-1 transcriptional activity. Although X/XO did not modify extracellular signal-regulated protein kinases 1/2 MAPK or Akt/PKB activity, it promote...
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PMID: 17278978
PDF is available here.
PDF is available here.
Molecular Microbiology (63)3 2007
Abstract
We show that the small subunit of the clamp loader replication factor C (RFC) of S. solfataricus interacted with both the catalytic and non-catalytic subunits of the primase by yeast two-hybrid and co-immunoprecipitation assays. Further, the primase-RFC interaction was also identified in the cell ex...
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PMID: 17181784
PDF is available here.
PDF is available here.
Nucleic Acids Research (35)9 2007
Abstract
We analyzed the accumulation of endogenous and fluorescent-tagged proteins for DNA repair synthesis at the sites of DNA damage produced locally by UVA-laser micro-irradiation in HeLa cells. Accumulation kinetics and in vitro pull-down assays of the large subunit of RFC (RFC140) revealed that there a...
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PMID: 17439963
PDF is available here.
PDF is available here.
Nucleic Acids Research (35)5 2007
Abstract
We present the co-crystal structure of yeast PCNA with a peptide encompassing the conserved PCNA interaction motif of Cdc9, yeast DNA ligase I. The Cdc9 peptide contacts both the inter-domain connector loop (IDCL) and residues near the C-terminus of PCNA. Complementary mutational and biochemical res...
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PMID: 17308348
PDF is available here.
PDF is available here.
Nucleic Acids Research (35)20 2007
Abstract
We describe dynamic properties of these proteins in the elongation step on a single-stranded M13 template, providing evidence that pol delta has a distributive nature over the 7 kb of the M13 template, repeating a frequent dissociation-association cycle at growing 3'-hydroxyl ends. Some PCNA could r...
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PMID: 17932049
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (281)46 2006
Abstract
We propose that this event is coupled to PCNA closure around DNA, which starts an ordered hydrolysis around the ring. PCNA closure severs contact to RFC subunits D and E (RFC2 and RFC5), and the gamma-phosphate sensor of ATP site C is switched off, resulting in low affinity of RFC for DNA and ejecti...
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PMID: 16980295
PDF is available here.
PDF is available here.
Abstract
We have developed RepairPATH, a computational algorithm that explores the activated signaling pathways in cancer. The RepairPATH integrates RepairNET, an assembled protein interaction network associated with DNA damage response, with the gene expression profiles derived from the microarray data. Bas...
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PMID: 16838343
PDF is available here.
PDF is available here.
Abstract
We now understand much better our own health problems. In some cases, we can modify our lifestyle and diet in order to prevent "environmental factors" from triggering the mutated genes inherited from our parents. Good examples are diabetes and cardiovascular diseases. If we realize we might have inh...
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PMID: 17087397
PDF is available here.
PDF is available here.
Abstract
Sliding clamps are ring-shaped proteins that tether DNA polymerases to DNA, which enables the rapid and processive synthesis of both leading and lagging strands at the replication fork. The clamp-loading machinery must repeatedly load sliding-clamp factors onto primed sites at the replication fork....
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PMID: 16955075
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (281)38 2006
Abstract
The heterotrimeric checkpoint clamp comprises the Saccharomyces cerevisiae Rad17, Mec3, and Ddc1 subunits (Rad17/3/1, the 9-1-1 complex in humans). This DNA damage response factor is loaded onto DNA by the Rad24-RFC (replication factor C-like complex with Rad24) clamp loader and ATP. Although Rad24-...
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PMID: 16864589
PDF is available here.
PDF is available here.
Molecular Carcinogenesis (45)8 2006
Abstract
We examined the impact of AP-1 activation on antiproliferative activity of ITCs, using bladder cancer cells and phenethyl isothiocyanate (PEITC) as models. AP-1 transactivation induced by PEITC was almost completely suppressed by a dominant-negative c-jun (TAM67). However, suppression of AP-1 transa...
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PMID: 16652374
PDF is available here.
PDF is available here.
Abstract
We show that human MutLalpha is a latent endonuclease that is activated in a mismatch-, MutSalpha-, RFC-, PCNA-, and ATP-dependent manner. Incision of a nicked mismatch-containing DNA heteroduplex by this four-protein system is strongly biased to the nicked strand. A mismatch-containing DNA segment...
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PMID: 16873062
PDF is available here.
PDF is available here.
Abstract
A great many carefully designed experiments will be required to fully understand biological mechanisms in atomic detail. A complementary approach is to use powerful statistical procedures to rapidly test numerous scientific hypotheses using vast numbers of protein sequences--the cell's own blueprint...
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PMID: 16766187
PDF is available here.
PDF is available here.
Anesthesia & Analgesia (103)1 2006
Abstract
We performed the present in vivo study to investigate the effect of hydroxyethyl starch (HES) on intestinal production of inflammatory mediators and activation of transcription factors during endotoxemia. Rats with endotoxemia induced by lipopolysaccharide (LPS) (5 mg/kg, IV) were treated with HES (...
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PMID: 16790644
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (281)25 2006
Abstract
We examined the roles of individual RFC subunits in opening the PCNA clamp. Interestingly, Rfc1, which occupies the position analogous to the delta clamp-opening subunit in the Escherichia coli clamp loader, is not required to open PCNA. The Rfc5 subunit is required to open PCNA. Consistent with thi...
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PMID: 16608854
PDF is available here.
PDF is available here.
Experimental Cell Research (312)6 2006
Abstract
We have previously shown that the regulatory subunit of PKA, RIalpha, functions as a nuclear transport protein for the second subunit of the replication factor C complex, RFC40, and that this transport appears to be crucial for cell cycle progression from G1 to S phase. In this study, we found that...
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PMID: 16413017
PDF is available here.
PDF is available here.
EMBO Journal (25)7 2006
Abstract
We report that Ctf18, Ctf8, and Dcc1, the subunits of a Replication Factor C (RFC)-like complex, are essential for the perinuclear positioning of telomeres. In both yeast and mammalian cells, peripheral nuclear positioning of chromatin during G1 phase correlates with late DNA replication. We find th...
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PMID: 16525505
PDF is available here.
PDF is available here.
Abstract
We capture an open yeast PCNA clamp in a complex with RFC through fluorescence energy transfer experiments. We also follow the topological transitions of PCNA in the various steps of the clamp-loading pathway through both steady-state and stopped-flow fluorescence studies. We find that ATP effective...
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PMID: 16476998
PDF is available here.
PDF is available here.
Abstract
We show that residues 375-480, which include 28 amino acids N-terminal to the BRCT domain, are required for 5'-phosphorylated double-stranded DNA binding. NMR chemical shift analysis indicated that the N-terminal extension includes an alpha-helix and confirmed the presence of a conserved BRCT domain...
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PMID: 16361700
PDF is available here.
PDF is available here.
Abstract
Our studies suggest that RNS may induce S-phase arrest through mechanisms that differ from those elicited by classical DNA-damaging agents....
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PMID: 16413407
PDF is available here.
PDF is available here.
Methods in Enzymology (408) 2006
Abstract
I (EXOI), replication protein A (RPA), proliferating cell nuclear antigen (PCNA), and replication factor C (RFC) is sufficient to support mismatch-provoked excision in vitro. This chapter describes methods for analysis of the reconstituted excision reaction....
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PMID: 16793375
PDF is available here.
PDF is available here.
Nucleic Acids Research (34)17 2006
Abstract
We report the fidelity of DNA synthesis in vitro by yeast pol zeta alone and with RFC, PCNA and RPA. Overall, the accessory proteins have little effect on the fidelity of pol zeta. Pol zeta is relatively accurate for single base insertion/deletion errors. However, the average base substitution fidel...
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PMID: 16971464
PDF is available here.
PDF is available here.