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Egg Proteins (6):
Recent article
Egg Proteins
Proteomics (11)1 2011
Abstract
Egg storage causes egg white to lose its viscous nature to form a thin liquid, commonly referred to as egg white thinning. To understand the mechanisms underlying egg white thinning, white-shell eggs were used in the present study to determine the proteome-level changes of egg white...
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PMID: 21182201
PDF is available here.
PDF is available here.
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Abstract
We utilized three different types of TiO(2) nanoparticles (NPs) namely TiO(2)-dopamine, TiO(2)-CdS and bare TiO(2) NPs as multifunctional nanoprobes for the rapid enrichment of phosphopeptides from tryptic digests of α- and β-casein, milk and egg white using a simplified procedure in MALDI-TOF-MS....
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PMID: 20928925
PDF is available here.
PDF is available here.
Genetics Research (92)4 2010
Abstract
TP5, a P element inserted in the telomere-associated sequences of the X chromosome, represses the excision of other P elements in the germ line through a combination of maternal and zygotic effects. The maternal component of this repression is impaired by heterozygous mutations in the aubergine and...
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PMID: 20943007
PDF is available here.
PDF is available here.
Abstract
The CpG island Exon 1 region of the Zygote arrest 1 (ZAR1) gene, which is responsible for oocyte-to-embryo transition, showed frequent aberrant methylation of 28 out of 30 (93%) melanoma surgical specimens, 16 of 17 (94%) melanoma cell lines, 0% of 4 normal human epidermal melanocyte (NHEM) cell lin...
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PMID: 20654783
PDF is available here.
PDF is available here.
Abstract
At fertilization, mouse sperm bind to the zona pellucida (which consists of glycoproteins ZP1, ZP2, and ZP3) that surrounds eggs. A ZP2 cleavage model of gamete recognition requires intact ZP2, and a glycan release model postulates that zona glycans are ligands for sperm. These two models were teste...
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PMID: 20616279
PDF is available here.
PDF is available here.
Abstract
We show that the same female provides both large and small mating partners with eggs of similar size, weight and lipid content when mated to two males in succession. Importantly, however, eggs provided to small males (less preferred) had higher egg protein content (11% more) than those provided to l...
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PMID: 20106851
PDF is available here.
PDF is available here.
Abstract
Anaphylaxis after eating sea urchin roe has been reported. However, its major allergens have not yet been identified. The aim of this study was to identify the major allergens of sea urchin roe. Proteins of sea urchin roe were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis a...
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PMID: 20526538
PDF is available here.
PDF is available here.
Swiss medical weekly : official journal of the Swiss Society of Infectious Diseases, the Swiss Society of Internal Medicine, the Swiss Society of Pneumology (140)17-18 2010
Abstract
Concerns about possible reactions to vaccines or vaccinations are frequently raised. However, the rate of reported vaccine-induced adverse events is low and ranges between 4.8-83.0 per 100,000 doses of the most frequently used vaccines. The number of true allergic reactions to routine vaccines is no...
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PMID: 20349363
PDF is available here.
PDF is available here.
Reproduction (139)5 2010
Abstract
We summarize our previous studies of the oocyte proteome, and present new panels of oocyte proteins that we recently identified in screens of metaphase II-arrested mouse oocytes. Importantly, our studies indicate that several abundant oocyte proteins are not, as one might predict, ubiquitous houseke...
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PMID: 20106898
PDF is available here.
PDF is available here.
Abstract
These results suggest that thermal processing may affect the quantitative analysis of allergens and needs to be taken into account in the validation of commercial ELISA test kits....
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PMID: 20349964
PDF is available here.
PDF is available here.
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases (28)2 2010
Abstract
DNA sequencing confirmed that the nucleotide sequence of synthesized IPSE gene was completely identical to the native one. SDS-PAGE showed that the recombinant plasmid IPSE/pET32a(+) expressed a fusion protein with an Mr 35700 after being induced by IPTG. The pure fusion protein Trx-IPSE reacted pos...
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PMID: 20666307
PDF is available here.
PDF is available here.
Journal of Proteomics (73)5 2010
Abstract
Combinatorial peptide ligand libraries have recently allowed considerable advances in the mapping of chicken egg yolk and white proteomics. Data from literature have been regrouped and elaborated for network and pathway analyses in order to convey a unified view of these proteomes. R...
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PMID: 20080220
PDF is available here.
PDF is available here.
Abstract
We compared immunochemical, DNA-based, and proteomic methods for detecting egg allergens in foods using egg allergen standards such as egg whole protein, egg white protein, egg yolk protein, ovomucoid, ovalbumin, ovotransferrin, lysozyme, and alpha-livetin. Protein-based immunochemical methods, incl...
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PMID: 20480891
PDF is available here.
PDF is available here.
Abstract
Using specific egg yolk antibodies (IgY), a strip-based immunochromatographic assay was developed for rapid detection of morphine in urine samples. IgY type antibody against morphine was generated by immunizing chickens with well-characterized monoacetyl morphine-protein conjugate. The antibody was...
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PMID: 19699078
PDF is available here.
PDF is available here.
Abstract
To evaluate the importance of the quantitative composition of vitellogenin (Vtg)-derived egg yolk proteins during initial events of Dentex dentex life, viable eggs were obtained from captive broodstocks. Viability parameters (Vps; egg buoyancy (FR), hatching rate (HR), and larval survival rate (SR)...
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PMID: 19810215
PDF is available here.
PDF is available here.
Parasitology (85 (Pt 2))02 2009
Abstract
The fine structure and histochemistry of the envelopes surrounding the mature oncosphere of Hymenolepis diminuta have been investigated by transmission electron microscopy and light microscope observations of JB-4 embedded material. The fate of these envelopes during the hatching sequence in vitro h...
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PMID: 6183635
PDF is available here.
PDF is available here.
Abstract
We present a method to determine the concentration of both the apo and holo forms of the riboflavin-binding protein (RFBP) present in avian egg white, by measuring the circular dichroism (CD) related to the controlled addition of riboflavin (or vitamin B2) to the egg white. At the same time, front-f...
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PMID: 19722562
PDF is available here.
PDF is available here.
Environmental Entomology (38)4 2009
Abstract
Diaphorina citri Kuwayama (Hemiptera: Psyllidae) is an important pest of citrus. It is an efficient vector of three bacterial pathogens that are the presumptive causal agents of huanglongbing (HLB) or citrus greening disease. The movement patterns and dispersal capabilities of D. citri require study...
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PMID: 19689907
PDF is available here.
PDF is available here.
Abstract
Human zona pellucida (hZP) plays a critical role in the recognition, binding of sperms and oocytes, induction of acrosomal exocytosis, and avoidance of polyspermy. Human ZP is composed of four glycoproteins designated as hZP1, hZP2, hZP3 and hZP4. This paper reviews the actions of native hZP or reco...
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PMID: 19852281
PDF is available here.
PDF is available here.
Current Biology (19)14 2009
Abstract
We show that the vitelline membrane-like (VML) protein undergoes Pipe-dependent sulfation and, consistent with a role in conveying positional information from the egg chamber to the embryo, becomes incorporated into the eggshell at a position corresponding to the location of the follicle cells from...
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PMID: 19540119
PDF is available here.
PDF is available here.
Abstract
We studied female-male coevolution in the abalone by resequencing sperm lysin and its interacting egg coat protein, VERL, in populations of two species. As predicted, we found intergenic linkage disequilibrium between lysin and VERL, despite our demonstration that they are not physically linked. Thi...
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PMID: 19629160
PDF is available here.
PDF is available here.
Developmental Biology (331)1 2009
Abstract
We report a novel function of PEM independently of its function in cleavage regulation; it controls cell fate by excluding competence to respond to the FGF signal for notochord induction from posterior-vegetal cells. PEM was found to regulate the nuclear accumulation of beta-catenin, an upstream act...
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PMID: 19406117
PDF is available here.
PDF is available here.
Abstract
A higher percentage of baculovirus-expressed recombinant human ZP3 coated beads showed significant (P<0.05) binding of capacitated human sperm as compared to beads coated with fetuin. An inhibition in the binding of sperm to ZP3 coated beads was observed in presence of cold recombinant human ZP3. Fu...
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PMID: 19700799
PDF is available here.
PDF is available here.
Hereditas (Beijing) (31)6 2009
Abstract
These results indicated that the expression of phiC31 integrase can be driven by ZP3 promoter efficiently and phiC31 integrase can mediate the site-specific recombination between attP site and attB site in mouse GV-stage oocytes. It could be a powerful tool for the study of recombination of specific...
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PMID: 19586858
PDF is available here.
PDF is available here.
Investigation of the protein-protein aggregation of egg white proteins under pulsed electric fields.
Abstract
Egg whites were exposed to pulsed electric fields (PEFs) to investigate the protein-protein aggregation. No insoluble protein aggregate was found when egg whites were exposed to PEFs at 25, 30, and 35 kV/cm for 400 micros. However, atomic force microscopy showed that the sizes of the protein particl...
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PMID: 19309077
PDF is available here.
PDF is available here.
Animal Reproduction Science (111)2-4 2009
Abstract
The avian inner perivitelline layer (IPVL) contains zona pellucida protein-B1 (ZPB1), zona pellucida protein-C (ZPC) and zona pellucida protein-D (ZPD). These three proteins may be involved in sperm binding to the IPVL. ZPB1 is produced by the liver and transported to the developing preovulatory fol...
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PMID: 18372124
PDF is available here.
PDF is available here.
Reproductive biology (9)1 2009
Abstract
We evaluated the transcript levels of integrins (alphaL, alphaM, beta1, and beta6), CD9 and CD18 antigens as well as zona pellucida glycoproteins (pZP1, pZP2, pZP3 and pZP3alpha) in oocytes isolated from puberal gilts (n=20) and multiparous sows (n=20). We found significantly (p<0.05) higher transcr...
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PMID: 19352419
PDF is available here.
PDF is available here.
Abstract
We focus on gel-based proteomic analysis of C. elegans subjected to two specific stress conditions during development: induction of the dauer state for whole body protein expression and a temperature shift for egg protein expression. Utilizing these differently perturbed C. elegans protein samples,...
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PMID: 19381582
PDF is available here.
PDF is available here.
Animal Genetics (40)1 2009
Abstract
Novel and traditional eggshell quality measurements were made from up to 2000 commercial pedigree hens for a candidate gene association analysis with organic eggshell matrix genes: ovocleidin-116, osteopontin (SPP1), ovocalyxin-32 (RARRES1), ovotransferrin (LTF), ovalbumin and ovocalyxin-36, as well...
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PMID: 18828860
PDF is available here.
PDF is available here.
Abstract
Our results showed that in vitro digestion of cooked eggs could generate a number of potent ACE inhibitory peptides which may have implications for cardiovascular disease prevention, including hypertension....
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PMID: 19154160
PDF is available here.
PDF is available here.
Abstract
Natural Eggshell Membrane (NEM) is a possible new effective and safe therapeutic option for the treatment of pain and inflexibility associated with joint and connective tissue (JCT) disorders. Supplementation with NEM, 500 mg taken once daily, significantly reduced pain, both rapidly (seven days) an...
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PMID: 19554094
PDF is available here.
PDF is available here.
Abstract
The present study investigated the global pattern of two histone modifications and methylation of DNA during in vitro maturation of bovine oocytes retrieved from follicles of two different sizes (<2 mm and 2-8 mm). The methylation status of histone H3 at position lysine K9 (H3K9 me2), the acetylatio...
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PMID: 19567217
PDF is available here.
PDF is available here.
Abstract
Abstract
Antigen-specific chicken IgY antibodies have been used for oral immunotherapy as an alternative or complement to antibiotics in several studies. The water dilution (WD) method has several advantages for purifying IgY. It is rapid, efficient, suitable for large-scale production, and nothing but water...
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PMID: 19053374
PDF is available here.
PDF is available here.
Abstract
We examined the effects of estrogens administered to sea urchin embryos and larvae. A typical malformation was exogastrulation, induced by the exposure to ethynylestradiol (EER) in a defined period of 12 h from 12 h after fertilization (HAF). Morphogenesis for gastrulation was delayed in the treated...
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PMID: 18459060
PDF is available here.
PDF is available here.
Abstract
These results highlight the potential of antihypertensive peptides to be used in the formulation of functional foods....
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PMID: 18727013
PDF is available here.
PDF is available here.
Abstract
Abstract
We describe the 2.3 ångström (A) resolution structure of the ZP-N fragment of mouse primary sperm receptor ZP3. The ZP-N fold defines a new immunoglobulin superfamily subtype with a beta-sheet extension characterized by an E' strand and an invariant tyrosine residue implicated in polymerization. T...
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PMID: 19052627
PDF is available here.
PDF is available here.
Abstract
We examined the preventive activity of naturally occurring antioxidants against three reactive oxygen species using a protein degradation assay. The hydroxyl, hypochlorite, and peroxynitrite radicals are typical reactive oxygen species generated in human body. Previously, we found that hydrophobic b...
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PMID: 19060409
PDF is available here.
PDF is available here.
Abstract
We introduce the diversity and generality of the control of sperm fertility....
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PMID: 18622578
PDF is available here.
PDF is available here.
Abstract
The prawn Macrobrachium borellii has lecithotrophic eggs with highly-abbreviated development. The major yolk component is lipovitellin (LV), a lipoprotein with 30% lipids (by weight). LV consumption during embryogenesis was followed by ELISA and Western blot analysis using an anti-LV...
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PMID: 18721894
PDF is available here.
PDF is available here.
Hereditas (Beijing) (30)11 2008
Abstract
MYP (Major yolk protein) gene expression at transcription level in different stages of gonad of sea urchin Strongylocentrotus intermedius and hybrids (S. intermediusfemale symbolxS. nudusmale symbol) was analyzed by real-time RT-PCR. Based on normalization with 18S rRNA levels, the comparative quant...
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PMID: 19073555
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1784)11 2008
Abstract
We studied a 67-residue-long amphiphilic beta-sheet of lipovitellin previously suggested a suitable working model for studies of the lipid-binding behaviour of amphiphilic beta-sheet regions in apolipoprotein B-100 (apoB-100). We performed four molecular dynamics simulations with different starting...
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PMID: 18619564
PDF is available here.
PDF is available here.
Abstract
There are two hatching enzyme homologues in the zebrafish genome: zebrafish hatching enzyme ZHE1 and ZHE2. Northern blot and RT-PCR analysis revealed that ZHE1 was mainly expressed in pre-hatching embryos, whereas ZHE2 was rarely expressed. This was consistent with the results obtained in an experime...
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PMID: 19021768
PDF is available here.
PDF is available here.
Abstract
We show, for the first time to our knowledge, the identification of a neurotoxin of proteinaceous nature localized inside an egg. Egg extracts from the freshwater apple snail Pomacea canaliculata displayed a neurotoxic effect in mice upon intraperitoneal injection (i.p.) (LD50, 96h 2.3mg/kg). Egg pr...
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PMID: 18640143
PDF is available here.
PDF is available here.
Abstract
Teleost choriogenins, precursors of the inner layer subunits of the egg envelope, are regarded as sensitive biomarkers for estrogenic pollutants. In this study, two full-length cDNAs, omChgH and omChgL, which encode the choriogenin H and L forms, respectively, were isolated from a brackish medaka, O...
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PMID: 18048097
PDF is available here.
PDF is available here.
Abstract
We report the characterization of scalarin (SC), a glyco-lipo-carotenoprotein from the PVF of P. scalaris. SC was purified by ultracentrifugation and exclusion chromatography. It is the major egg protein, representing 64% of the total soluble protein. The particle has a hydration density of 1.26 g/m...
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PMID: 18213678
PDF is available here.
PDF is available here.
Poultry Science (87)8 2008
Abstract
We expanded our search to other skeletal tissues. Using Western blotting, we found OC-116 in the matrix of chick cortical bone and laying hen medullary bone as well as in hypertrophic chondrocyte lysates. Furthermore, other members of this family (bone sialoprotein, dental matrix protein-1, and oste...
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PMID: 18648057
PDF is available here.
PDF is available here.
BioTechniques (45)2 2008
Abstract
We show a simple system based on avian egg white that supports growth of cells in 3-D, at a significantly decreased cost. Specifically, we show that growth of immortalized human breast epithelial cells (MCF10A) in egg white-based medium results in formation of acini with hollow lumens, apoptotic cle...
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PMID: 18687065
PDF is available here.
PDF is available here.
Abstract
Abstract
Nonwoven poly(epsilon-caprolactone) (PCL) nanofibers were prepared by electrospinning technology, and a novel natural bioactive material, soluble eggshell membrane protein (SEP), which is made from natural eggshell membrane (ESM), was then immobilized on the nanofibers after the surf...
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PMID: 17969029
PDF is available here.
PDF is available here.
Abstract
The use of two types of peptide ligand libraries (PLL), containing hexapeptides terminating either with a primary amine or modified with a terminal carboxyl group, allowed the discovery and identification of a large number of previously unreported egg white proteins. Whereas the most...
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PMID: 18570458
PDF is available here.
PDF is available here.
Abstract
These results support a recent paradigm regarding the significance of Vg multiplicity upon successive physiological events in this group of fishes including the hydration of maturing oocytes, the acquisition of proper egg buoyancy, and the generation of requisite nutrient stocks for each stage of em...
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PMID: 18324616
PDF is available here.
PDF is available here.
Abstract
We have previously shown that the gilthead seabream ZP proteins are differentially transcribed in liver and ovary, with the expression in liver being under estrogenic control. However, although mRNA was found in both liver and ovary, only low ZPBa protein levels were detected in liver and plasma. Us...
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PMID: 18247334
PDF is available here.
PDF is available here.
Developmental Dynamics (237)8 2008
Abstract
We will discuss the amplification of the chorion gene clusters and the data acquired on the expression patterns and the regulatory elements controlling transcription of eggshell genes. We will then focus on the findings that correlate follicular epithelium patterning and eggshell gene expression, an...
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PMID: 18651659
PDF is available here.
PDF is available here.
Hormones and Behavior (54)3 2008
Abstract
We tested the hypothesis that the experience of female birds with humans could affect yolk steroids levels and offspring phenotype. Two groups of Japanese quail, one habituated to humans (H) and a second non-habituated (NH), were exposed daily to brief human disturbances. We analysed egg quality, of...
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PMID: 18572170
PDF is available here.
PDF is available here.
Veterinary Microbiology (130)1-2 2008
Abstract
The objective of this study was to estimate the in vitro activity of egg yolk immunoglobulin (IgY) against mastitis-causing Escherichia coli. Specific IgY was produced by hens immunized with formaldehyde killed E. coli O111 in long-standing immunization response (titer > or =6400 for 100 days) and w...
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PMID: 18255238
PDF is available here.
PDF is available here.
Abstract
We have developed a murine cytomegalovirus (MCMV)-vectored vaccine expressing the mouse zona-pellucida-3 gene (rMCMV-ZP3), which successfully induces infertility in experimentally inoculated laboratory or wild-derived mice. However, the future success of this vector as a fully disseminating vaccine...
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PMID: 18573574
PDF is available here.
PDF is available here.
Abstract
Abstract
Despite cyclopenta-fused polycyclic aromatic hydrocarbons (CP-PAHs) have been detected in the environment, the ability of CP-PAH to induce cellular and tissue responses remains poorly characterized. In this study, xenoestrogen-associated responses (mRNA levels of estrogen receptor alpha, ERalpha, an...
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PMID: 18538753
PDF is available here.
PDF is available here.