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Epididymal Secretory Proteins (0):
Recent article
Epididymal Secretory Proteins
Abstract
Allergen-mediated cross-linking of the high-affinity receptor for IgE on mast cells triggers the release of diverse preformed and de novo synthesized immunoregulatory mediators that further the allergic response. A proteomic screen applied to the detection of proteins secreted by the model rat mast...
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PMID: 20001954
PDF is available here.
PDF is available here.
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Clinical laboratory (56)11-12 2010
Abstract
Abstract
We conclude that the HE4 EIA from Fujirebio Diagnostic Inc. displayed convenient analytical performances that allows its use it clinical practice....
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PMID: 20478777
PDF is available here.
PDF is available here.
Abstract
The epididymis is an excellent target for the development of a male contraceptive. This is because the process of sperm maturation occurs in this organ; spermatozoa become motile and are able to recognise and fertilise an egg once they have traversed the epididymal duct. However, a number of attempt...
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PMID: 20839090
PDF is available here.
PDF is available here.
British Journal of Cancer (101)3 2009
Abstract
Cancer Research (69)15 2009
Abstract
We conducted preclinical tests in ovarian tumor-bearing mice to evaluate the therapeutic efficacy of using a cationic biodegradable poly(beta-amino ester) polymer as a vector for nanoparticulate delivery of DNA encoding a diphtheria toxin suicide protein (DT-A). The promoter sequences of two genes t...
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PMID: 19643734
PDF is available here.
PDF is available here.
Abstract
This study reports the first successful recombinant expression of cationic antimicrobial peptides human beta-defensin-26 and human beta-defensin-27 in Escherichia coli. HBD26 and HBD27 genes were synthesized through codon optimization, and each gene was then cloned into the expression vector pET32,...
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PMID: 19373462
PDF is available here.
PDF is available here.
Abstract
The ability of serum mesothelin, HE4, and CA 125 levels to discriminate ovarian cancer cases from healthy and benign controls is not influenced by risk status. Our findings support the pursuit of additional studies evaluating the early detection potential of these markers in high-risk populations....
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PMID: 19423517
PDF is available here.
PDF is available here.
Abstract
We conducted an association study of 551 glioma cases and 865 matched controls of European ancestry to investigate "tag" single nucleotide polymorphisms (SNP) in 148 genetic regions. Two independent U.S. case-control studies included were as follows: a hospital-based study conducted by the National...
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PMID: 19423540
PDF is available here.
PDF is available here.
Anticancer Research (29)2 2009
Abstract
Considering the multiplexed markers, only CA125(Luminex), osteopontin and IGF-II differed significantly between groups. Levels of all non-multiplexed tumour markers were significantly higher in the cancer group compare to the control. CONCLUSION: Multiplex is a powerful tool for multiparametric stud...
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PMID: 19331205
PDF is available here.
PDF is available here.
Gynecologic Oncology (112)1 2009
Abstract
Twelve sites enrolled 531 evaluable patients with 352 benign tumors, 129 EOC, 22 LMP tumors, 6 non EOC and 22 non ovarian cancers. The postmenopausal group contained 150 benign cases of which 112 were classified as low risk giving a specificity of 75.0% (95% CI 66.9-81.4), and 111 EOC and 6 LMP tumo...
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PMID: 18851871
PDF is available here.
PDF is available here.
Abstract
To investigate human epididymis protein 4 (HE4) levels in transitional cell carcinoma (TCC) of the urinary system and its relationship with clinicopathological features.
102 patients with TCC, 60 with benign urinary diseases, and 60 healthy controls were included in t...
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PMID: 19927341
PDF is available here.
PDF is available here.
Abstract
MS analysis showed that one of the most prominent proteins, M(r) 22 000, was identical to the phosphatidylethanolamine-binding protein (PBP), and it was further identified as PBP by immuno-blot assay. CONCLUSION: PBPs were present in a variety of molecular forms in the epididymal luminal fluid, incl...
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PMID: 19288743
PDF is available here.
PDF is available here.
Biology of Reproduction (79)6 2008
Abstract
We demonstrate that protein D associates transiently with the sperm surface in a concentration-dependent manner, exhibiting saturable binding to both caput and cauda sperm in a concentration range that is consistent with its capacitation-inhibiting activity. In contrast, protein E persists on the sp...
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PMID: 18703418
PDF is available here.
PDF is available here.
Human Reproduction (23)11 2008
Abstract
DEFB126 and its high negative charge appears to be critical for the movement of sperm through CM in the macaque, while SPPs adhered to the sperm surface offer no advantage in CMP....
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PMID: 18658160
PDF is available here.
PDF is available here.
Abstract
Sperm maturation in the epididymis is regulated by changes of luminal ion concentration and processing of sperm surface membrane by several glycosidases and proteases, and the actions of the proteases are controlled by protease inhibitors present in specific areas of the epididymis. WFDC-type serine...
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PMID: 19102507
PDF is available here.
PDF is available here.
Abstract
We hope to provide an overview of the early detection ovarian cancer from traditional methods to recent promises in the proteomics pipeline....
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PMID: 18468571
PDF is available here.
PDF is available here.
Animal Reproduction Science (107)3-4 2008
Abstract
We found that sperm harvested from different parts of the epididymis (caput, corpus and cauda) had a varying, but generally poor, ability to undergo the acrosome reaction in vitro. At ejaculation, however, sperm mix with seminal plasma which contains various components, including the small membranou...
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PMID: 18550301
PDF is available here.
PDF is available here.
Abstract
CA125, mesothelin, and HE4 levels in healthy, postmenopausal women at increased risk for ovarian cancer are significantly associated with a few ovarian cancer risk factors. Since the effects of these personal characteristics on these serum markers are not large, their incorporation in screening algo...
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PMID: 18768519
PDF is available here.
PDF is available here.
Gynecologic Oncology (110)2 2008
Abstract
Serum samples from 156 healthy subjects and 171 patients with endometrial cancer (122 stage I, 17 stage II, 26 stage III, and 6 stage IV) were analyzed. At a 95% specificity, the sensitivities for differentiating between healthy subjects and all stages of cancer were 45.5% for HE4 and 24.6% for CA12...
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PMID: 18495222
PDF is available here.
PDF is available here.
Biology of Reproduction (78)5 2008
Abstract
We have identified and characterized a novel glycoprotein (rabbit epididymal secretory protein 52 [REP52]) of 52 kDa. REP52 is synthesized and secreted in a tissue-specific manner by the mid (region 6) and distal (region 7) corpus epididymidis and associates weakly with the sperm surface overlying t...
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PMID: 18199886
PDF is available here.
PDF is available here.
Clinical Cancer Research (14)9 2008
Abstract
On average across analyte levels, Bb/pAb assays yielded within-run and between-run coefficients of variations of 11.7 and 23.8, respectively, for HE4 and 14.0 and 14.5, respectively, for mesothelin. In the subset (n = 214), Pearson correlations of 0.95 for HE4 and 0.92 for mesothelin were observed b...
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PMID: 18451228
PDF is available here.
PDF is available here.
Gastroenterology (134)2 2008
Abstract
Although traditionally metaplasia was thought to originate from normal mucosal progenitor cells, these studies indicate that SPEM evolves through either transdifferentiation of chief cells or activation of a basal cryptic progenitor. In addition, induction of metaplasia elicits the expression of sec...
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PMID: 18242217
PDF is available here.
PDF is available here.
Gynecologic Oncology (108)2 2008
Abstract
As a single tumor marker, HE4 had the highest sensitivity for detecting ovarian cancer, especially Stage I disease. Combined CA125 and HE4 is a more accurate predictor of malignancy than either alone....
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PMID: 18061248
PDF is available here.
PDF is available here.
Fertility and Sterility (89)1 2008
Abstract
OBJECTIVE: To evaluate the immunocontraceptive properties of recombinant DE, a sperm epididymal protein involved in fertilization, via an experimental study in rats as a critical step toward the development of a human immunocontraceptive. DESIGN: In vivo study in rats. SETTING: Animal care facility...
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PMID: 17482178
PDF is available here.
PDF is available here.
Abstract
We are presently studying their relationship to the patients' clinical state to learn whether measurements of antibody levels provide information that can aid diagnosis and monitoring of treated patients. Prospective studies are needed to establish the clinical relevance of our findings....
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PMID: 18546615
PDF is available here.
PDF is available here.
Journal of Immunological Methods (317)1-2 2006
Abstract
We describe here a novel method for generation of yeast-secreted, in vivo biotinylated recombinant antibodies, or biobodies. Biobodies are secreted by diploid yeast resulting from the fusion of two haploid yeast of opposite mating type. One yeast carries a cDNA encoding an antibody recognition seque...
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PMID: 17113097
PDF is available here.
PDF is available here.
Abstract
Present results reinforce that extracerebral sources of S100B, particularly adipocytes, contribute to its serum levels and support the idea that caution is needed when interpreting serum S100B increase as a clinical marker of brain damage....
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PMID: 16740441
PDF is available here.
PDF is available here.
Modern Pathology (19)6 2006
Abstract
We examined the expression of the HE4 gene and protein in a large series of normal and malignant adult tissues by oligonucleotide microarray and tissue microarray, respectively. HE4 gene expression was highest in normal human trachea and salivary gland, and to a lesser extent, lung, prostate, pituit...
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PMID: 16607372
PDF is available here.
PDF is available here.
Fertility and Sterility (85 Suppl 1) 2006
Abstract
Our results provide the first demonstration that in vivo gene transfer can be used to study the localization of proteins in mature sperm and that this represents a powerful new technique for studying male infertility and gene function in sperm....
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PMID: 16616103
PDF is available here.
PDF is available here.
Clinical Cancer Research (12)7 2006
Abstract
Suitability for use in a bead-based assay varied across CA125 antibody pairs. Two CA125 bead-based assays were highly reproducible (overall correlations between replicates >/= 0.95; coefficients of variation < 0.2) and strongly correlated with the research standard CA125II RIA (correlations >/= 0.9)...
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PMID: 16609024
PDF is available here.
PDF is available here.
Lancet Oncology (7)2 2006
Abstract
We assess data for an association between cancer and human WAP proteins, and discuss their potential role in tumour progression. We also propose a new mechanism by which WAP proteins might have a role in carcinogenesis....
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PMID: 16455481
PDF is available here.
PDF is available here.
Respiratory Research (7)1 2006
Abstract
WFDC2 is expressed in some epithelial cells of the upper airways as well as in mucous cells and ducts of submucosal glands. No staining was seen in peripheral lung. Intense staining is found in major salivary glands and in minor glands of the nose, sinuses, posterior tongue and tonsil. Studies with...
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PMID: 16600032
PDF is available here.
PDF is available here.
Abstract
The epididymis is a single and highly convoluted tubule system in mammals. The epithelium is the major compartment for epididymal function. Proteins synthesized and secreted by epididymal epithelium provide a special and ever-changing luminal fluid environment for sperm as they progress through the...
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PMID: 16483167
PDF is available here.
PDF is available here.
Biology of Reproduction (73)6 2005
Abstract
We used a novel in vivo assay to determine immune recognition: aldehyde-fixed whole sperm injections into rabbits. Following booster injections, immunoblot analyses of whole sperm prepared in various manners was conducted. On Days 60 and 80 post-initial immunization, the antisera showed a remarkably...
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PMID: 16079310
PDF is available here.
PDF is available here.
Abstract
We performed large-scale differential gene expression analysis on the skin of 5-d GC13 versus normal FVB mice using DNA chip assays. A surprising result of these experiments was the identification of the epididymal cysteine-rich secretory protein 1 (Crisp1) gene as one of the genes with the greatest...
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PMID: 16382673
PDF is available here.
PDF is available here.
Gynecologic Oncology (99)2 2005
Abstract
At the level of tissue expression, each of 10 potential serum markers could be detected in 29-100% of ovarian cancers that had low or absent expression of CA125. Several markers exhibited more intense expression in cancers than in normal organs. Further investigation is needed to demonstrate complem...
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PMID: 16061277
PDF is available here.
PDF is available here.
Journal of Membrane Biology (207)3 2005
Abstract
Based on the amino-acid sequence of the macaque epididymal secretory protein, ESP 13.2 (Q9BEE3/AJ236909), it has now been classified as beta-defensin DEFB126. DEFB126 is one of the five beta-defensins with genes that are clustered along chromosome 20pl3, and all five proteins have an extended carbox...
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PMID: 16550483
PDF is available here.
PDF is available here.
Cancer Research (65)6 2005
Abstract
We did a comprehensive assessment of HE4 protein expression in benign and malignant ovarian and nonovarian tissues by immunohistochemistry. In comparison with normal surface epithelium, which does not express HE4, we found that cortical inclusion cysts lined by metaplastic Mullerian epithelium abund...
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PMID: 15781627
PDF is available here.
PDF is available here.
Abstract
Our results suggest that ESP13.2 masks zona pellucida ligands on the sperm surface and its release, as part of capacitation, is required for sperm-zona interaction....
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PMID: 15349845
PDF is available here.
PDF is available here.
Abstract
Some of the genes we identified with differential expression are related to the immune system. These results are telling us the new information for understanding the secretory human endometrium....
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PMID: 15373944
PDF is available here.
PDF is available here.
Gynecologic Oncology (92)3 2004
Abstract
The HE4 promoter was active in 5/7 ovarian cancer cell lines with the range of activity spanning 0.06- to 3-fold that observed for a positive control, cotransfected reporter construct (SV-40-luc). Minimal pHE4-652 promoter activity, defined as < or =5% of the activity detected with the SV-40-luc con...
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PMID: 14984958
PDF is available here.
PDF is available here.
Abstract
Rodent ESTs and genomic sequences homologous to HE3, HE4 and Ce8/Ly6G5C were identified in the public databases and the "full-length" rat cDNAs cloned. To emphasise their homology to the human and canine genes, they were named Me3/Re3, Me4/Re4 and Re8 for mouse and rat counterparts, respectively. mR...
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PMID: 14695977
PDF is available here.
PDF is available here.
Cancer Research (63)13 2003
Abstract
We therefore performed experiments to explore whether quantitation of HE4 protein levels in serum can be used as a biomarker for ovarian carcinoma. A fusion gene was constructed encoding the HE4 protein fused to a gene encoding the murine IgG2a Fc domain. Subsequently, protein produced in mammalian...
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PMID: 12839961
PDF is available here.
PDF is available here.
Biology of Reproduction (69)1 2003
Abstract
The expression pattern of EP2 variants was examined in the rhesus monkey (Macaca mulatta). Using reverse transcriptase-polymerase chain reaction and rapid amplification of complementary cDNA protocols, 11 message variants were identified in rhesus epididymis, only three of which (EP2B, EP2C, and EP2...
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PMID: 12606416
PDF is available here.
PDF is available here.
Abstract
We investigated the participation of DE in two mechanisms probably involved in egg activation: the ability of DE to trigger activation by its interaction with the binding sites on the egg surface (receptor model) and its ability to regulate intracellular calcium channels (sperm factor model). The in...
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PMID: 12887093
PDF is available here.
PDF is available here.
Abstract
The final stages of sperm differentiation occur outside the gonad and are not under the genomic control of germ cells. Only sequential interactions with the medium surrounding the sperm are believed to induce the final steps of spermatogenesis. The epididymis, a long tubule with very active secretor...
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PMID: 12672118
PDF is available here.
PDF is available here.
Abstract
Northern blot analysis suggested that the boar epididymis produces closely related counterparts to human epididymal proteins HE1, HE3, HE4, HE5 and HE12. 'Full-length' cloning by nucleic acid and amino acid sequence similarity was achieved by RT-PCR methods in the case of the porcine counterparts of...
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PMID: 12654021
PDF is available here.
PDF is available here.
Reproduction (125)4 2003
Abstract
A rat epididymal protein of 27 kDa was identified using neonatal tolerization. This study reports the production and characterization of a polyclonal antiserum to this protein. ELISA was used to demonstrate that this antiserum reacts strongly with epididymal sperm proteins, but has little or no reac...
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PMID: 12683920
PDF is available here.
PDF is available here.
Biology of Reproduction (68)1 2003
Abstract
We report the identification of a novel epididymis-specific secretory glycoprotein, E-3, which is a sperm-associated isoantigen containing defensin- and lectin-like motifs. E-3 was detected in rat epididymal fluid and in sperm extracts by two-dimensional (2-D) Western blotting using rat hyperimmune...
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PMID: 12493725
PDF is available here.
PDF is available here.
Abstract
Journal of Biological Chemistry (277)19 2002
Abstract
We have previously indicated that at least in mouse, sperm serine protease(s) other than acrosin probably act on the limited proteolysis of egg zona pellucida to create a penetration pathway for motile sperm, although the participation of acrosin cannot be ruled out completely. A 42-kDa gelatin-hydr...
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PMID: 11861648
PDF is available here.
PDF is available here.
Abstract
We have shown that HE4 is expressed in a number of normal human tissues outside of the male reproductive system, including regions of the respiratory tract and nasopharynx, as well as in a subset of lung tumour cell lines. Comparison of multiple HE4 cDNAs and RT-PCR products with genomic sequence al...
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PMID: 11965550
PDF is available here.
PDF is available here.
Abstract
Primary cultures of the differentiated, adult epididymal duct epithelium were immortalized by retroviral transduction with the simian virus (SV)40 large T antigen. The canine epididymis was chosen here as a model with high human relevance, representing a convenient and acceptable source of different...
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PMID: 11574662
PDF is available here.
PDF is available here.
Journal of Andrology (22)1 2001
Abstract
The rat Crisp-1 gene encodes Protein DE (acidic epididymal glycoprotein; AEG), a glycoprotein secreted by the epididymal epithelium that associates with maturing sperm and has been implicated in the process of sperm-egg fusion. Previous characterization of the Crisp-1 messenger RNA in the rat epidid...
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PMID: 11191082
PDF is available here.
PDF is available here.
Abstract
Androgen dependent epididymal proteins act as antigen to produce autoantibodies and affect normal fertility. In the present study, epididymal proteins were analyzed during the time of sexual maturation and their androgen dependency was studied in male albino mice. Epididymis of 21 days (Pre-pubertal...
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PMID: 11811849
PDF is available here.
PDF is available here.
Biology of Reproduction (63)2 2000
Mammalian sperm-egg fusion: evidence that epididymal protein DE plays a role in mouse gamete fusion.
Abstract
We provide evidence supporting a role for DE in mouse sperm-egg fusion. Western blot studies indicated that the antibody against rat protein DE can recognize the mouse homologue in both epididymal tissue and sperm extracts. Indirect immunofluorescence studies using this antibody localized the protei...
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PMID: 10906051
PDF is available here.
PDF is available here.
Abstract
We examined the association of DE with the sperm surface and the relationship of this interaction with the behavior and function of the protein. Cloning and sequencing of DE revealed a lack of hydrophobic domains and the presence of 16 cysteine residues in the molecule. Experiments in which cauda ep...
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PMID: 10813850
PDF is available here.
PDF is available here.
Abstract
We determined that these LESP genes are differentially expressed during the annual epididymal cycle....
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PMID: 10657004
PDF is available here.
PDF is available here.
Neuroscience (95)4 2000
Abstract
We have used the technique of messenger RNA differential display to identify genes with altered expression in these neurons which may contribute to the development of aberrant sensation following such peripheral nerve damage. This approach identified 14 distinct complementary DNA clones, representin...
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PMID: 10682718
PDF is available here.
PDF is available here.