Find your PDFs fast.
Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Flavoproteins (20):
- Nitrate Reductase (NAD(P)H)
- Acyl-CoA Dehydrogenase, Long-Chain
- Acyl-CoA Dehydrogenase
- Methylenetetrahydrofolate Reductase (NADPH2)
- Thioredoxin-Disulfide Reductase
- Electron-Transferring Flavoproteins
- Dihydrolipoamide Dehydrogenase
- Cytochrome-B(5) Reductase
- Acetolactate Synthase
- Sarcosine Oxidase
- Retinal Dehydrogenase
- Acyl-CoA Oxidase
- Glutamate Synthase (NADH)
- NADPH Oxidase
- Butyryl-CoA Dehydrogenase
- Apoptosis Inducing Factor
- Nitrate Reductase (NADPH)
- Nitrate Reductase (NADH)
- NADH Dehydrogenase
- Flavodoxin
Recent article
Flavoproteins
Neuroreport (21)13 2010
Abstract
We used flavoprotein autofluorescence imaging to show intact thalamocortical connectivity of nonspecific nuclei in slice preparations of the somatosensory and auditory systems. These preparations will enable the elucidation of electrophysiological properties of nonspecific pathways....
|
PMID: 20647961
PDF is available here.
PDF is available here.
| < More recent | Older article > |
Plant Physiology (153)3 2010
Abstract
We show that the monomeric protein contains one noncovalently bound flavin adenine dinucleotide (FAD) that is essential for enzyme activity only in the presence of NADPH, which functions as the FAD reductant. The reaction proceeds without transfer of hydrogen from the dinucleotide cofactors to beta-...
|
PMID: 20460582
PDF is available here.
PDF is available here.
Abstract
We observed in both cases the ultrafast photoreduction of FAD(ox): in 390 fs for OtCPF1 and 590 fs for OtCPF2. Although such ultrafast electron transfer has already been reported for other flavoproteins and CPF members, the present result is the first demonstration with full spectral characterizatio...
|
PMID: 20222748
PDF is available here.
PDF is available here.
Abstract
BMC Microbiology (10) 2010
Abstract
We showed that a fur deletion mutant of S. oneidensis could utilize TCA compounds. Consistently, expression of the TCA cycle genes acnA and sdhA was not down-regulated in the mutant. To explore this observation further, we identified a ryhB gene in Shewanella species and experimentally demonstrated...
|
PMID: 20950482
PDF is available here.
PDF is available here.
Nature Neuroscience (13)1 2010
Abstract
We found that the corticothalamocortical pathway drove robust activity in higher-order somatosensory cortex. When the direct corticocortical pathway was interrupted, secondary somatosensory cortex showed robust activity in response to stimulation of the barrel field in primary somatosensory cortex (...
|
PMID: 19966840
PDF is available here.
PDF is available here.
Abstract
We compared the changes in the FPF and somatosensory evoked potential (SEP) during transient cerebral ischemia in the rat. We measured the FPF and SEP simultaneously via a cranial window made over the right sensorimotor cortex during the left median nerve stimulation in F344 rats. We compared change...
|
PMID: 20204777
PDF is available here.
PDF is available here.
Abstract
We monitored faster, light-induced changes in absorption of an algal cryptochrome covering a spectral range of 375-750 nm with a streak camera setup. Electron transfer from tryptophan to flavin is completed before 100 ns under formation of the flavin anion radical. Proton transfer takes place with a...
|
PMID: 19754110
PDF is available here.
PDF is available here.
Biochemistry (Washington) (48)40 2009
Abstract
We postulate that mutation of Tyr258 causes subtle changes in active site dynamics that promote release of the reactive dihydropicolinate intermediate and disrupt the efficient synchronization of oxygen activation observed with wild-type nikD....
|
PMID: 19702312
PDF is available here.
PDF is available here.
Biochemistry (Washington) (48)36 2009
Abstract
We describe the purification of type 4 CRYs of zebrafish and chicken as recombinant proteins with full flavin complement and compare the spectroscopic properties of type 4 and type 1 CRYs. In addition, we analyzed photoinduced proteolytic degradation of both types of CRYs in vivo in heterologous sys...
|
PMID: 19663499
PDF is available here.
PDF is available here.
Biochemistry (Washington) (48)32 2009
Abstract
We construct LOV2 domains with (LOV-Jalpha) and without (LOV-core) the Jalpha helix for Arabidopsis phot1 and phot2 and Adiantum neochrome 1 and compare their light-induced difference FTIR spectra. Light-induced protein structural changes differ significantly between LOV-Jalpha and LOV-core for Arab...
|
PMID: 19601589
PDF is available here.
PDF is available here.
Anesthesia & Analgesia (109)2 2009
Abstract
Preconditioning of cardiomyocytes with desflurane or sevoflurane significantly decreased oxidative stress-induced cell death. That effect coincided with increased ROS production and increased flavoprotein oxidation detected during acute myocyte exposure to the anesthetics. Desflurane induced signifi...
|
PMID: 19608810
PDF is available here.
PDF is available here.
Abstract
We show that the rhythmic mRNA expression of clock genes (Clock, Bmal1, Cry1, and Dbp) is not attenuated in the liver and visceral adipose tissues of Goto-Kakizaki rats, a model of nonobese, type 2 diabetes, as compared to control Wistar rats. Our results suggest that molecular clock impairment in p...
|
PMID: 19387896
PDF is available here.
PDF is available here.
Biophysical Journal (97)1 2009
Abstract
We study the effects of temperature and hydration on the light-triggered signal propagation in the phot1 LOV2 domain of Avena sativa (AsLOV2/Jalpha), using Fourier transform infrared spectroscopy to unravel part of the molecular mechanism of phototropin 1. We report that AsLOV2/Jalpha shows an inten...
|
PMID: 19580761
PDF is available here.
PDF is available here.
Biophysical Journal (97)1 2009
Abstract
We conclude that the FMN T1 state remains nonprotonated on a nanosecond timescale, which rules out an ionic mechanism for covalent adduct formation involving cysteine-N(5) proton transfer on this timescale. Finally, we observed a heterogeneous population of singly and doubly H-bonded FMN C(4)=O conf...
|
PMID: 19580760
PDF is available here.
PDF is available here.
Biophysical Journal (96)12 2009
Abstract
We investigate here a possible radical pair-based reaction in the photoreceptor cryptochrome that reduces the protein's flavin group from its signaling state FADH* to the inactive state FADH- (which reacts to the likewise inactive FAD) by means of the superoxide radical, O2*-. We argue that the spin...
|
PMID: 19527640
PDF is available here.
PDF is available here.
EMBO Reports (10)6 2009
Abstract
We show that the PtCPF1 protein from the marine diatom Phaeodactylum tricornutum shows 6-4 photoproduct repair activity and can act as a transcriptional repressor of the circadian clock in a heterologous mammalian cell system. Conversely, it seems to have a wide role in blue-light-regulated gene exp...
|
PMID: 19424294
PDF is available here.
PDF is available here.
Abstract
We demonstrated that expression of the clock and clock-controlled genes showed robust rhythms in mouse skin under constant dark conditions, whereas these rhythms were completely lost in Cry1/Cry2 knockout mice lacking a molecular clock. At the cellular level, the main oscillatory protein in the mamm...
|
PMID: 19037239
PDF is available here.
PDF is available here.
Abstract
We hypothesized that variations in the fluorescence of mitochondrial nicotinamide adenine dinucleotide (NADH) and flavoprotein (FP) can be used acutely to predict the degree of myocardial injury. Thirteen rabbits had coronary occlusion for 30 min followed by 3 h of reperfusion. To produce a spectrum...
|
PMID: 19272908
PDF is available here.
PDF is available here.
Biochemical Journal (419)2 2009
Abstract
In low nanomolar concentrations, NO (nitric oxide) functions as a transmitter in brain and other tissues, whereas near-micromolar NO concentrations are associated with toxicity and cell death. Control of the NO concentration, therefore, is critical for proper brain function, but, although its synthe...
|
PMID: 19152507
PDF is available here.
PDF is available here.
Biochemical Society transactions (37)Pt 2 2009
Abstract
We report on nuclear-spin polarized tryptophan resonances that are generated by light-induced intraprotein electron transfer to the FMN cofactor. The spectra are discussed with respect to earlier data obtained from (13)C-NMR experiments on unlabelled LOV2 domains that have been reconstituted with FM...
|
PMID: 19290867
PDF is available here.
PDF is available here.
Swiss medical weekly : official journal of the Swiss Society of Infectious Diseases, the Swiss Society of Internal Medicine, the Swiss Society of Pneumology (139)13-14 2009
Abstract
As the porphyria specialist centre of Switzerland, we perform the specialized analyses required for the diagnosis of all types of porphyrias, and give advice to patients, physicians and other laboratories. We therefore estimated that our data cover 80-90% of all diagnosed Swiss cases. A total of 217...
|
PMID: 19350426
PDF is available here.
PDF is available here.
Human Genetics (125)3 2009
Abstract
Helicobacter (14)2 2009
Abstract
Background and Aims: Helicobacter pylori infection is associated with a variety of diseases including gastric cancer. Flavodoxin is an electron transfer protein containing a flavin mononucleotide prosthetic group and substituted an iron-containing electron transfer protein under iron-limiting condit...
|
PMID: 19298342
PDF is available here.
PDF is available here.
Abstract
Abstract
We compared the ontogenetic maturation of the clockwork in the SCN and pars tuberalis (PT). The PT is a peripheral oscillator that strongly depends on rhythmic melatonin signals. Immunoreactions for clock gene proteins were determined in the SCN and PT at four different timepoints during four differ...
|
PMID: 19222558
PDF is available here.
PDF is available here.
Abstract
We observe a trapped flavin C4a−OH or C4a−OO(H) adduct by single-crystal spectroscopic methods and in the 1.86 Å resolution X-ray crystal structure of choline oxidase. The microspectrophotometry results show that the adduct forms rapidly in situ at 100 K upon exposure to X-rays. Density functio...
|
PMID: 19133805
PDF is available here.
PDF is available here.
EMBO Journal (28)2 2009
Abstract
Phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) is needed for retrograde membrane trafficking from lysosomal and late endosomal compartments and its synthesis is tightly regulated. But how cells regulate PtdIns(3,5)P2 synthesis--for example, in response to hyperosmotic shock--remains unexplain...
|
PMID: 19158662
PDF is available here.
PDF is available here.
EMBO Journal (28)2 2009
Abstract
We show that significant reduction in RNA polymerase II-dependent transcription did not abolish circadian oscillations, but surprisingly accelerated them. A similar period shortening was observed at reduced incubation temperatures in wild-type mouse fibroblasts, but not in cells lacking Per1. Our da...
|
PMID: 19078963
PDF is available here.
PDF is available here.
EMBO Journal (28)2 2009
Abstract
A thought-provoking study in this issue of The EMBO Journal shows that the circadian clock in mouse fibroblasts is surprisingly insensitive to the inhibition of total cellular mRNA production. The authors go on to show intriguing parallels between compensation of period to changes in temperature and...
|
PMID: 19158661
PDF is available here.
PDF is available here.
Abstract
Journal of Biology (8)6 2009
Abstract
The annual migration of the Monarch butterfly (Danaus plexippus) from eastern North America to central Mexico is one of nature's most inspiring spectacles. Recent studies including one in BMC Biology, have begun to dissect the molecular and neurogenetic basis for this most complex behavior.
|
PMID: 19591650
PDF is available here.
PDF is available here.
Postepy biochemii (55)2 2009
Abstract
Chloroplast redistribution in the cell depends on direction, fluence-rate and spectral composition of the incident light. Two photoreceptors, phototropin 1 and 2, control the chloroplast responses in higher land plants. Actin and myosin form the motor system. Although numerous results point to calci...
|
PMID: 19824475
PDF is available here.
PDF is available here.
Abstract
Under study were features of allele polymorphism of genes of methylenetetrahydrofolate reductase (MTHFR C677T and A1298C), methionine synthase (MS A 2756G), methionine synthase reductase (MTRR A66G) and methylenetetrahydrofolate dehydrogenase (MTHFD G1958A) in patients with atherosclerosis of the lo...
|
PMID: 20209990
PDF is available here.
PDF is available here.
Abstract
The mouse aldehyde oxidase AOH2 (aldehyde oxidase homolog 2) is a molybdoflavoenzyme. Harderian glands are the richest source of AOH2, although the protein is detectable also in sebaceous glands, epidermis, and other keratinized epithelia. The levels of AOH2 in the Harderian gland and skin are contr...
|
PMID: 18981221
PDF is available here.
PDF is available here.
FEBS Letters (583)1 2009
Abstract
Two flavo-diiron proteins (FDPs), FprA1 and FprA2, are up-regulated when the strictly anaerobic solvent producer, Clostridium acetobutylicum, is exposed to dioxygen. These two FDPs were purified following heterologous overexpression in Escherichia coli as N-terminal Strep-tag fusion proteins. The re...
|
PMID: 19084524
PDF is available here.
PDF is available here.
Abstract
We report here an investigation of the function and expression of two cryptochromes in soybean, GmCRY1a and GmCRY2a. Soybean is a short-day (SD) crop commonly cultivated according to the photoperiodic sensitivity of cultivars. Both cultivated soybean (Glycine max) and its wild relative (G. soja) exh...
|
PMID: 19106300
PDF is available here.
PDF is available here.
Biochemistry (Washington) (47)52 2008
Abstract
We designed two point mutations, Q513L and Q513N, and monitored the effects on the chromophore and protein using a combination of UV-visible absorbance and circular dichroism spectroscopy, limited proteolysis, and solution NMR. The results show that these mutations significantly dampen the changes b...
|
PMID: 19063612
PDF is available here.
PDF is available here.
Journal of Physical Chemistry A: Molecules, Spectroscopy, Kinetics, Environment and General Theory (112)50 2008
Abstract
We employed classical molecular dynamics free energy simulations (MDFES), within a thermodynamic integration (TI) formalism, to calculate the change in FMN first reduction potential (DeltaDeltaE(o)(ox/sq)) imparted by 6 flavoprotein active site mutations. The combined performance of the AMBER ff03 (...
|
PMID: 18828581
PDF is available here.
PDF is available here.
The photoreversible fluorescent protein iLOV outperforms GFP as a reporter of plant virus infection.
Abstract
We have engineered a smaller ( approximately 10 kDa) flavin-based alternative to GFP ( approximately 25 kDa) derived from the light, oxygen or voltage-sensing (LOV) domain of the plant blue light receptor, phototropin. Molecular evolution and Tobacco mosaic virus (TMV)-based expression screening pro...
|
PMID: 19060199
PDF is available here.
PDF is available here.
EMBO Journal (27)24 2008
Abstract
We predict that yeast and mammalian Vac14 are composed entirely of HEAT repeats and demonstrate that Vac14 exerts an effect as a scaffold for the PI(3,5)P(2) regulatory complex by direct contact with the known regulators of PI(3,5)P(2): Fig4, Fab1, Vac7 and Atg18. We also report that the mouse mutan...
|
PMID: 19037259
PDF is available here.
PDF is available here.
Abstract
Abstract
We have expressed the mature form of human DMGDH and the H109R variant identified in a DMGDH-deficient patient as N-terminally His(6)-tagged proteins in E. coli. The enzymes were purified to homogeneity by nickel affinity and anion exchange chromatography. The presence of FAD in the wild-type enzyme...
|
PMID: 18937046
PDF is available here.
PDF is available here.
Plant and Cell Physiology (49)12 2008
Abstract
Branch position in the moss Physcomitrella patens is regulated by blue light. In this study, fluence rate dependency of branch position determination was investigated by partial cell irradiation with a microbeam. With a 30 Wm(-2) or lower fluence rate, branches formed at the microbeam area, but form...
|
PMID: 18930957
PDF is available here.
PDF is available here.
Molecular Carcinogenesis (47)12 2008
Abstract
We analyzed differential expression of the 9 circadian genes in 46 hepatocellular carcinoma and paired noncancerous tissues by real-time quantitative RT-PCR and immunohistochemical detection. We also tested the possible regulatory mechanism(s) by direct sequencing and methylation PCR analysis. Our r...
|
PMID: 18444243
PDF is available here.
PDF is available here.
Journal of Bacteriology (190)24 2008
Abstract
We uncovered a hierarchical relationship between these two regulatory systems, earlier thought to function independently. We also more accurately assessed the spectrum of gene targets of the PrrBA system. First, expression of the appA gene, encoding the PpsR antirepressor, is PrrA dependent, which e...
|
PMID: 18931128
PDF is available here.
PDF is available here.
Abstract
We applied fluorescence line narrowing (FLN), resonance Raman (RR) and Fourier-transform infrared (FTIR) spectroscopy to investigate the electronic structure of FMN bound to Avena sativa LOV2 (AsLOV2), its C450A mutant and Adiantum LOV2 (Phy3LOV2). We demonstrate that FLN is the method of choice to...
|
PMID: 18989482
PDF is available here.
PDF is available here.
Biochemistry (Washington) (47)44 2008
Abstract
Flavoredoxin is a FMN-containing electron transfer protein that functions in the energy-yielding metabolism of Desulfovibrio gigas of the Bacteria domain. Although characterization of this flavoredoxin is the only one reported, a database search revealed homologues widely distributed in both the Bac...
|
PMID: 18842001
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (283)45 2008
Abstract
I calculated E(m) for Tyr-21, flavin, and redox-active Trp-104 and revealed the electron transfer (ET) driving energy. Rotation of the Gln-63 side chain that converts protein conformation from the dark state to the light state is responsible for the decrease of 150 mV in E(m) for Tyr-21, leading to...
|
PMID: 18647748
PDF is available here.
PDF is available here.
Abstract
We report the in vitro reconstitution of this four-protein system and identify the final product as (1S,2R)-allocoronamic acid bound in thioester linkage to KtzC. Further analysis of KtzD and KtzA support a biosynthetic pathway that involves KtzD-mediated generation of a gamma-chloroisoleucyl interm...
|
PMID: 18828590
PDF is available here.
PDF is available here.
Abstract
The aim of this work was to investigate the effect of the in vitro circadian-like exposure to melatonin [in the presence or absence of insulin (Ins)] on the metabolism and clock gene expression in adipocytes. To simulate the cyclic characteristics of the daily melatonin profile, isolated rat adipocy...
|
PMID: 18662218
PDF is available here.
PDF is available here.
Relationship between AMPK and the transcriptional balance of clock-related genes in skeletal muscle.
Abstract
We show the AMP-activated protein kinase (AMPK)gamma(3) subunit is involved in the regulation of peripheral circadian clock function. AMPKgamma3 knockout (Prkag3(-/-)) mice or wild-type littermates were injected with saline or an AMPK activator, 5-amino-4-imidazole-carboxamide riboside (AICAR), and...
|
PMID: 18728219
PDF is available here.
PDF is available here.
Abstract
We reported that certain variants in circadian genes were associated with risk for prostate cancer. To provide some biological insight into these findings, we examined the relationship of five variants of circadian genes, including NPAS2 (rs2305160:G > A), PER1 (rs2585405:G > C), CSNK1E (rs1005473:A...
|
PMID: 18990770
PDF is available here.
PDF is available here.
Plant Journal (56)3 2008
Abstract
We exchanged the N- and C-terminal moieties of phot1 and phot2, fused them to GFP and expressed them under the PHOT2 promoter in the phot1 phot2 mutant background. With respect to phototropism and other responses, the chimeric phototropin consisting of phot1 N-terminal and phot2 C-terminal moieties...
|
PMID: 18643969
PDF is available here.
PDF is available here.
Biochemistry (Washington) (47)43 2008
Abstract
We show for the first time that Rnf is a flavoprotein, with FMN covalently bound to threonine-175 in RnfG and a second flavin bound to threonine-187 in RnfD. Rnf subunits D and G are homologous to subunits B and C of Na+-NQR, respectively. Each of these Na+-NQR subunits includes a conserved S(T)GAT...
|
PMID: 18831535
PDF is available here.
PDF is available here.
Abstract
Strongly spin-polarized 13C NMR lines have been observed upon photoexcitation of FMN-binding LOV domains from the blue-light receptor phototropin. Their origin can be rationalized in terms of intermediate radical-pair spin chemistry. Due to hyperfine-selective branching into singlet and triplet prod...
|
PMID: 18798620
PDF is available here.
PDF is available here.
Abstract
We tested this idea by creating PAS-DHFR, a designed chimeric protein that connects a light-sensing signaling domain from a plant member of the Per/Arnt/Sim (PAS) family of proteins with Escherichia coli dihydrofolate reductase (DHFR). With no optimization, PAS-DHFR exhibited light-dependent catalyt...
|
PMID: 18927392
PDF is available here.
PDF is available here.
Journal of Neuroscience Research (130)39 2008
Abstract
We were able to stabilize two semiquinone radicals and thus observed their weak emission spectra. Significantly, we observed a strong correlation between their excited-state dynamics and the planarity of their flavin isoalloxazine ring. For a bent ring structure, we observed ultrafast dynamics from...
|
PMID: 18767842
PDF is available here.
PDF is available here.
Abstract
We now show that Fab1, through its chaperonin-like domain, binds to Vac14 and Fig4 and forms a vacuole-associated signaling complex. The Fab1 complex is tethered to the vacuole via an interaction between the FYVE domain in Fab1 and PtdIns(3)P on the vacuole. Moreover, Vac14 and Fig4 bind to each oth...
|
PMID: 18653468
PDF is available here.
PDF is available here.
Plant and Cell Physiology (49)10 2008
Abstract
We show that in PixD, Met93, the residue conserved in all BLUF domains, is crucial for light-dependent signal transduction. Specifically, the light-insensitive M93A mutant of PixD revealed biochemical and physiological activities compatible with those of the light-adapted wild-type PixD. However, th...
|
PMID: 18772185
PDF is available here.
PDF is available here.