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Fungal Proteins (3):
Recent article
Fungal Proteins
Abstract
We found that the core ER-lumenal domain (cLD) of yeast Ire1 binds to unfolded proteins in yeast cells and to peptides primarily composed of basic and hydrophobic residues in vitro. Mutation of amino acid side chains exposed in a putative peptide-binding groove of Ire1 cLD impaired peptide binding....
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PMID: 21852455
PDF is available here.
PDF is available here.
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Eukaryotic Cell (10)9 2011
Cell wall chitosan is necessary for virulence in the opportunistic pathogen Cryptococcus neoformans.
Abstract
We demonstrate chitosan is necessary for virulence and persistence in the mammalian host....
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PMID: 21784998
PDF is available here.
PDF is available here.
Fungal Biology (115)8 2011
Abstract
We report the cloning and characterization of the cDNA encoding the ACO of P. brasiliensis (PbACO). The cDNA showed a 2361 bp open reading frame (ORF) and encoded a predicted protein with 787 amino acids. Polyclonal antibodies against the purified recombinant PbACO was obtained in order to analyze t...
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PMID: 21802049
PDF is available here.
PDF is available here.
PLoS Genetics (7)6 2011
Abstract
We chart a high-resolution map of natural trait variation in one of the most important genetic model organisms, the budding yeast Saccharomyces cerevisiae, and its closest wild relatives and trace the genetic basis and timing of major phenotype changing events in its recent history. We show that nat...
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PMID: 21698134
PDF is available here.
PDF is available here.
Fungal Biology (115)6 2011
Abstract
We address key aspects of the versatile fungal cytoskeleton, highlight recently gained insights into important roles of F-actin in filamentous fungi, and raise some key questions that are likely to be solved in the coming years based on the new experimental tools that have recently become available....
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PMID: 21640316
PDF is available here.
PDF is available here.
Fungal Biology (115)6 2011
Abstract
We show here that in mating projections of both C. albicans and S. cerevisiae a Spitzenkörper-like structure is present at the growing tip and a band of septin bars is present at the base. Furthermore, in S. cerevisiae mating projections, Spa2 and Bni1 form a cap to the 3-dimensional ball of FM4-64...
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PMID: 21640318
PDF is available here.
PDF is available here.
Xenobiotica (41)5 2011
Abstract
We provide a sample preparation method for quantitative proteomics of cellular fungi. Two different protein extraction methods were compared with focus on reproducibility, minimized proteolytic degradation and protein losses during the sample preparation. In the first preparation the cells were lyse...
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PMID: 18369902
PDF is available here.
PDF is available here.
DNA Repair (10)5 2011
Abstract
We use two protein footprinting techniques, limited proteolysis and oxidative surface mapping, coupled with mass spectrometry to identify amino acids involved along the DNA-binding surface of the Pms1-NTD. Limited proteolysis experiments elucidated several basic residues that were protected in the p...
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PMID: 21354867
PDF is available here.
PDF is available here.
Abstract
We propose several novel algorithms to reconstruct the growth history of a present-day network. Our likelihood-based method finds a probable previous state of the graph by applying an assumed growth model backwards in time. This approach retains node identities so that the history of individual node...
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PMID: 21533211
PDF is available here.
PDF is available here.
Cell Host & Microbe (9)3 2011
Abstract
We have determined that Gat201 controls the mRNA levels of ∼1100 genes (16% of the genome) and binds the upstream regions of ∼130 genes. Seven Gat201-bound genes encode for putative and known transcription factors--including two previously implicated in virulence--suggesting an extensive regulat...
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PMID: 21402362
PDF is available here.
PDF is available here.
Eukaryotic Cell (10)3 2011
Abstract
We characterized Swi6p, Swi4p and Mbp1p, the closest orthologues of SBF (Swi6p and Swi4p) and MBF (Swi6p and Mbp1p) components in S. cerevisiae. The mbp1Δ/Δ cells showed minor growth defects, whereas swi4Δ/Δ and swi6Δ/Δ yeast cells dramatically increased in size, suggesting a G(1) phase delay....
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PMID: 21257795
PDF is available here.
PDF is available here.
Abstract
Glycerol is a by-product of biodiesel production. Currently, it has limited applications with low bioconversion efficiency to most metabolites reported. This is partly attributed to the poor knowledge on the glycerol metabolic pathway in bacteria and fungi.
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PMID: 21305026
PDF is available here.
PDF is available here.
Abstract
We isolated 18 P. capsici NPP genes, of which 12 were active during hypha growth from a Phytophthora stain isolated from pepper (Capsicum annuum) plants in China. The 18 predicted proteins had a sequence homology of 46.26%. The 18 Pcnpp sequences had a conserved GHRHDWE motif and fell into two group...
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PMID: 21644208
PDF is available here.
PDF is available here.
Abstract
We examined the contribution of these integrated pathways to the growth and virulence of A. fumigatus, focusing on the ERAD protein DerA and the master regulator of the UPR, HacA. A ΔderA mutant grew normally and showed no increase in sensitivity to ER stress. However, expression of the UPR target...
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PMID: 21217201
PDF is available here.
PDF is available here.
Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.] (Chapter 3) 2011
Abstract
Reaction conditions for a variety of endonucleases are detailed in this unit along with discussions of potential applications. Enzymes covered include BAL 31 nuclease, S1 nuclease, mung bean nuclease, micrococcal nuclease, and DNase I. A general discussion regarding the use of endonu...
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PMID: 21225639
PDF is available here.
PDF is available here.
Abstract
We show that a strain carrying a deletion of cre-1 has increased cellulolytic activity and increased expression of cellulolytic genes during growth on crystalline cellulose (Avicel). Constitutive expression of cre-1 complements the phenotype of a N. crassa Δcre-1 strain grown on Avicel, and also re...
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PMID: 21980519
PDF is available here.
PDF is available here.
BMC Systems Biology (5 Suppl 1) 2011
Abstract
Identification of gene regulatory networks is useful in understanding gene regulation in any organism. Some regulatory network information has already been determined experimentally for model organisms, but much less has been identified for non-model organisms, and the limited amount...
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PMID: 21689479
PDF is available here.
PDF is available here.
Abstract
We recently found sympatric pairs of Saccharomyces species, composed of one cryotolerant and one thermotolerant species in oak bark samples of various geographic origins. In order to contribute to explain the occurrence in sympatry of Saccharomyces species, we screened Saccharomyces genomic data for...
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PMID: 21674061
PDF is available here.
PDF is available here.
Abstract
We have recently shown that, in vivo, glucose regulates V-ATPase through cytosolic pH, suggesting that V-ATPase contains a pH sensitive subunit, which regulates assembly of the holo-complex.(2) Here, we present the purification and biochemical characterization of the N-terminal domain of subunit 'a'...
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PMID: 21057203
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (285)53 2010
Abstract
We analyzed the role of Tom7 in assembly of β-barrel proteins, including Tom40, a central channel subunit of the TOM40 complex, and porin. Depletion of Tom7 decreased transient accumulation of Tom40 at the level of the TOB complex and retarded assembly of porin in vitro. On the other hand, overexpr...
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PMID: 21036907
PDF is available here.
PDF is available here.
Waste Management (31)1 2011
Abstract
Our results showed a potential viable and economic use of vinasse.
Copyright © 2010 Elsevier Ltd. All rights reserved....
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PMID: 20864326
PDF is available here.
PDF is available here.
Microbial Pathogenesis (49)6 2010
Abstract
We investigated if some NSAIDs increment or diminish the secretion of aspartyl-proteases (Sap) by Candida albicans grown under different phenotypes and oxygen availability using a set of SAP knock-out mutants and other set for genes (EFG1 and CPH1) that codify transcription factors involved in filam...
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PMID: 20708674
PDF is available here.
PDF is available here.
Abstract
We show that native kinetochore particles retaining the majority of kinetochore proteins can be purified from budding yeast and used to reconstitute dynamic microtubule attachments. Individual kinetochore particles maintain load-bearing associations with assembling and disassembling ends of single m...
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PMID: 21107429
PDF is available here.
PDF is available here.
Trends in Microbiology (18)12 2010
Abstract
We should study it.
Copyright © 2010 Elsevier Ltd. All rights reserved....
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PMID: 21035346
PDF is available here.
PDF is available here.
Abstract
The pathogenic mechanisms of fungal infection during human keratomycosis were investigated in an ex vivo corneal model that used strains of Fusarium oxysporum differing in the production of a fungal transcription factor.
A pacC loss-of-function mutant and a pacC dominant-activating mutant were const...
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PMID: 20856109
PDF is available here.
PDF is available here.
EMBO Journal (29)23 2010
Abstract
We show that an N-terminal region of Spt6 (Spt6N) is responsible for interaction with Iws1. The crystallographic structures of Encephalitozoon cuniculi Iws1 and the Iws1/Spt6N complex reveal two conserved binding subdomains in Iws1. The first subdomain (one HEAT repeat; HEAT subdomain) is a putative...
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PMID: 21057455
PDF is available here.
PDF is available here.
Abstract
We show that under conditions where sexual development is inhibited, a ∼17-fold excess of MFα pheromone transcript is synthesized and then degraded by a DEAD box protein, Vad1, resulting in low steady state transcript levels. Transfer to mating medium or deletion of the VAD1 gene resulted in high...
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PMID: 20801870
PDF is available here.
PDF is available here.
Journal of Proteomics (73)12 2010
Abstract
We find that the only fining agent used is bovine casein, just like in white wines. Although the typical levels of casein found range between 45 to 85μg/L, in one case as little as 3.8μg/L of casein could be detected, an extremely high level of sensitivity, close to our lower detection limit of 1...
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PMID: 20813213
PDF is available here.
PDF is available here.
Abstract
I of SQS is essential for binding of the diphosphate substrate....
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PMID: 20585832
PDF is available here.
PDF is available here.
Fungal Genetics and Biology (47)11 2010
Abstract
We show that RCO-1 and RCM-1, the Neurospora homologs of the components of the yeast Tup1-Ssn6 repressor complex, participate in photoadaptation. Mutation in either rco-1 or rcm-1 result in high and sustained accumulation of mRNAs for con-10 and other light-regulated genes after long exposures to li...
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PMID: 20709620
PDF is available here.
PDF is available here.
Fungal Genetics and Biology (47)11 2010
Abstract
The agaricomycete (homobasidiomycete) Coprinopsis cinerea has been used as a model to study the molecular mechanism for photomorphogenesis. Molecular genetic analyses of mutants defective in fruiting body (mushroom) photomorphogenesis of C. cinerea identified two genes, dst1 and dst2. dst1 encodes a...
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PMID: 20471485
PDF is available here.
PDF is available here.
Fungal Genetics and Biology (47)11 2010
Abstract
Virtually all organisms exposed to light are capable of sensing this environmental signal. In recent years the photoreceptors that mediate the ability of fungi to "see" have been identified in diverse species, and increasingly characterized. The small sizes of fungal genomes and ease in genetic and...
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PMID: 20451644
PDF is available here.
PDF is available here.
Fungal Genetics and Biology (47)11 2010
Abstract
Light is an environmental signal that modulates many aspects of the biology of zygomycete fungi. Light regulation has been investigated in the zygomycetes Phycomyces blakesleeanus, Mucor circinelloides and Pilobolus crystallinus. Examples of light regulation include the phototropism of the fruiting...
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PMID: 20466063
PDF is available here.
PDF is available here.
Eukaryotic Cell (9)11 2010
Abstract
Cryptococcus neoformans is an encapsulated yeast that causes a life-threatening meningoencephalitis in immunocompromised individuals. The ability to survive and proliferate at the human body temperature is an essential virulence attribute of this pathogen. This trait is controlled in...
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PMID: 20889719
PDF is available here.
PDF is available here.
Eukaryotic Cell (9)11 2010
Abstract
We characterize the upstream developmental regulator A. fumigatus flbB (AfuflbB). Northern blotting and cDNA analyses reveal that AfuflbB produces two transcripts predicted to encode two basic leucine zipper domain (bZIP) polypeptides, AfuFlbBβ (420 amino acids [aa]) and AfuFlbBα (390 aa). The del...
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PMID: 20852021
PDF is available here.
PDF is available here.
Abstract
These results demonstrate that cell wall from MTV can be used to differentially bind E. coli spp. and Salmonella spp. up to 8×10(4) CFU/10 μg. Thus certain yeast cell walls may prevent enteric infections caused by selective bacteria. This methodical approach would be an accurate tool in the feed i...
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PMID: 20826190
PDF is available here.
PDF is available here.
Proteomics (10)19 2010
Abstract
We have applied a 2-DE and MS approach to identify 117 proteins from the National Center of Biotechnology Information nonredundant protein database and a custom database of Basidiomycota EST sequences. Proteins with roles in primary metabolism, energy transduction, stress, cellular regulation and si...
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PMID: 20821732
PDF is available here.
PDF is available here.
Abstract
We isolated and characterized a novel Ras small monomeric GTPase Rsr1 gene, designated PmRsr1, from yeast-form Penicillium marneffei. The full-length PmRsr1 cDNA sequence is 1,866 bp in size, and contains an open reading frame of 642 bp encoding 213 amino acids. The predicted molecular mass of PmRsr...
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PMID: 20058189
PDF is available here.
PDF is available here.
Cell Host & Microbe (8)3 2010
Abstract
We demonstrate that oral epithelial cells orchestrate an innate response to C. albicans via NF-κB and a biphasic MAPK response. Activation of NF-κB and the first MAPK phase, constituting c-Jun activation, is independent of morphology and due to fungal cell wall recognition. Activation of the sec...
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PMID: 20833374
PDF is available here.
PDF is available here.
Abstract
We identified a new isolated naringinase-producing yeast strain named as Jmudeb008, and analyzed its naringinase-producing ability cultured with different composition and concentration of carbon sources.
The strain was identified based on conventional phenotypic methods and sequences of the D1/D2 re...
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PMID: 21090260
PDF is available here.
PDF is available here.
Abstract
These results suggested that the T. harzianum isolates had high levels of genetic variation compared to the other isolates of Trichoderma species....
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PMID: 20890090
PDF is available here.
PDF is available here.
Food Microbiology (27)6 2010
Abstract
We used a reverse transcription-quantitative PCR (RT-qPCR) method with relative quantification to investigate the expression of seven hexose transporter genes (HXT1-7) and one invertase-encoding gene (SUC2) during ADY rehydration in water with or without sucrose. For this, seven candidate reference...
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PMID: 20630323
PDF is available here.
PDF is available here.
Fungal Biology (114)9 2010
Abstract
We partially sequenced single copy nuclear genes encoding translation elongation factor 1-α (TEF), reductase (RED) and phosphate permease (PHO) in 100 F. culmorum isolates with 11 isolates of Fusarium crookwellense, Fusarium graminearum and Fusarium pseudograminearum. Phylogenetic analysis of multi...
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PMID: 20943185
PDF is available here.
PDF is available here.
Fungal Biology (114)9 2010
Abstract
A hypocrealean Coleoptera pathogen with characteristic part-spores, collected from Khao Yai National Park and Kaeng Krachan National Park in Thailand, is reported. The overall morphology was similar to Cordyceps barnesii, which is known from Sri Lanka, with ascospores disarticulating...
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PMID: 20943183
PDF is available here.
PDF is available here.
Eukaryotic Cell (9)9 2010
Abstract
We employed the same strategy to analyze the role of Rfg1p in filamentation and virulence. Studies using a tet-RFG1 strain revealed that RFG1 overexpression does not inhibit hypha formation in vitro or in the mouse model of hematogenously disseminated candidiasis. Interestingly, RFG1 overexpression...
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PMID: 20656914
PDF is available here.
PDF is available here.
Eukaryotic Cell (9)9 2010
Abstract
We have previously shown that deletion of cspA is associated with rapid conidial germination and reduced adhesion of dormant conidia. Here we show that cspA can be extracted with hydrofluoric acid (HF) from the cell wall, suggesting that it is a GPI-anchored CWP. The cspA-encoded CWP is unmasked dur...
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PMID: 20656913
PDF is available here.
PDF is available here.
Eukaryotic Cell (9)9 2010
Abstract
We have previously observed that constitutive expression of UME6, which encodes a filament-specific transcriptional regulator, is sufficient to direct hyphal growth in the absence of filament-inducing conditions. Here we show that HGC1, encoding a cyclin-related protein necessary for hyphal growth u...
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PMID: 20656912
PDF is available here.
PDF is available here.
Eukaryotic Cell (9)9 2010
Abstract
We were unable to determine a possible role for melanin in the virulence of C. albicans. However, we showed that melanin was externalized from the fungal cells in the form of electron-dense melanosomes that were free or often loosely bound to the cell wall exterior. Melanin production was boosted by...
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PMID: 20543065
PDF is available here.
PDF is available here.
Proteomics (10)17 2010
Abstract
We describe the proteome and transcriptome of planktonic- and biofilm-grown A. fumigatus mycelium after 24 and 48 h. A biofilm- and time-dependent regulation of many proteins and genes of the primary metabolism indicates a developmental stage of the young biofilm at 24 h, which demands energy. At a...
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PMID: 20645385
PDF is available here.
PDF is available here.
Mycopathologia (170)3 2010
Abstract
We introduce a putatively hyperactive truncated Afyap1 into wild-type A. fumigatus. We found that the resulted A. fumigatus containing truncated Afyap1 attenuated susceptibility to voriconazole and resistance to various oxidants. However, the Afyap1 deletion mutant and the strain harboring multiple...
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PMID: 20376564
PDF is available here.
PDF is available here.
Biophysical Journal (99)4 2010
Abstract
We previously generated a toxic mutant of the nontoxic HET-s((218-289)) amyloid in yeast. Here we report that toxic and nontoxic amyloids differ not only in their structures but also in their assembling process. We used multiple and complementary methods to investigate the intermediates formed by th...
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PMID: 20713008
PDF is available here.
PDF is available here.
Abstract
We tested the impact of FKS1 modulation on susceptibility to additional antifungal classes. Reduction of FKS1 expression rendered biofilms more susceptible to amphotericin B, anidulafungin, and flucytosine. Increased resistance to anidulafungin and amphotericin B was observed for biofilms overexpres...
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PMID: 20516280
PDF is available here.
PDF is available here.
Abstract
The relationship between susceptibilities to fluconazole and itraconazole and microsatellite CAI genotypes were examined from a total of 154 Candida albicans isolates (97 isolates causing vulvovaginitis in Chinese women and 6 vaginal isolates and 51 oral cavity isolates from asymptomatic carriers)....
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PMID: 20516286
PDF is available here.
PDF is available here.
Abstract
In the absence of exogenous electron donors monofunctional heme peroxidases can slowly degrade hydrogen peroxide following a mechanism different from monofunctional catalases. This pseudo-catalase cycle involves several redox intermediates including Compounds I, II and III, hydrogen peroxide reducti...
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PMID: 20434429
PDF is available here.
PDF is available here.
Abstract
The ability to resist anti-microbial compounds is of key evolutionary benefit to microorganisms. Aspergillus niger has previously been shown to require the activity of a phenylacrylic acid decarboxylase (encoded by padA1) for the decarboxylation of the weak-acid preservative sorbic acid (2,4-hexadie...
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PMID: 20452450
PDF is available here.
PDF is available here.
Abstract
I (RPB1) and nuclear ribosomal large subunit (LSU) rDNA gene sequences. Based on the result of this initial screen, 62 collections representing the full range of genetic diversity sampled were subjected to multigene phylogenetic species recognition based on genealogical concordance (GCPSR). The 62-t...
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PMID: 20580850
PDF is available here.
PDF is available here.
Abstract
The internal hydrostatic pressure (turgor) of the filamentous fungus Neurospora crassa is regulated at about 400-500 kiloPascals, primarily by an osmotic MAP kinase cascade which activates ion uptake from the extracellular medium and glycerol synthesis. In the absence of hyperosmotic stress, the phe...
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PMID: 20546911
PDF is available here.
PDF is available here.
Phytophthora nicotianae transformants lacking dynein light chain 1 produce non-flagellate zoospores.
Abstract
We report the cloning and characterization of DLC1 homologues in Phytophthora cinnamomi and Phytophthora nicotianae (PcDLC1 and PnDLC1). PcDLC1 and PnDLC1 are single copy genes that are more highly expressed in sporulating hyphae than in vegetative hyphae, zoospores or germinated cysts. Polyclonal a...
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PMID: 20451645
PDF is available here.
PDF is available here.
Abstract
We propose that the reduced virulence of the pga29 mutant is a consequence of altered surface properties, resulting in altered fungal recognition.
Copyright (c) 2010 John Wiley & Sons, Ltd....
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PMID: 20533408
PDF is available here.
PDF is available here.
Abstract
We here present a first systematic analysis of the cell wall composition of the milk yeast, Kluyveromyces lactis. Electron microscopic analyses revealed that exponentially growing cells of K. lactis supplied with glucose as a carbon source have a wall thickness of 64 nm, as compared to 105 nm when g...
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PMID: 20641021
PDF is available here.
PDF is available here.