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Globulins (3):
Recent article
Globulins
Abstract
11S globulin is one of the major seed storage proteins in amaranth. Recombinant protein was produced as up to approximately 80% of the total bacterial protein using Escherichia coli Rosetta-gami (DE3) containing pET21d with amaranth 11S globulin cDNA. The best expression condition was at 302 K for 2...
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PMID: 20693668
PDF is available here.
PDF is available here.
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Abstract
Validated murine models have been built to assess the potential allergenicity of novel proteins. Large animals, such as pigs, share more similarities to humans in physiology and immunology than murine. Among Chinese minipigs, Wuzhishan (WZS) minipigs have the highest inbreeding coefficient, more sta...
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PMID: 20369852
PDF is available here.
PDF is available here.
Abstract
Our results suggest that a small dose of lipoic acid has the potential to be an immunomodulator to prevent soybean-induced allergies....
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PMID: 20302377
PDF is available here.
PDF is available here.
Abstract
Our goal was to evaluate if protein fractions from cacao seeds show antitumor activity on lymphoma murine L5178Y model. The antioxidant activity of these fractions was also evaluated with the aim of finding a correlation with the antitumor activity.
Differential extraction of proteins from unferment...
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PMID: 20961452
PDF is available here.
PDF is available here.
Abstract
We have assessed the effect of gender on short-term calorie restriction influencing body weight and immunological parameters in albino rats. Adult albino rats (12 females, 12 males) were taken for the study and randomly divided into control and experimental groups of both the genders (n = 6 in each)...
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PMID: 21046919
PDF is available here.
PDF is available here.
Materiae Vegetabiles (64)4 2009
Abstract
Consumption of soybeans can reduce the risk of different types of cancer. Little is known about the effect of germination on the anticancer properties of soya. This study was done to determine if germination improves the anticancer properties of soybean protein through generation of...
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PMID: 19688264
PDF is available here.
PDF is available here.
Materiae Vegetabiles (64)4 2009
Abstract
Consumption of soybeans can reduce the risk of different types of cancer. Little is known about the effect of germination on the anticancer properties of soya. This study was done to determine if germination improves the anticancer properties of soybean protein through generation of...
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PMID: 19688264
PDF is available here.
PDF is available here.
Materiae Vegetabiles (64)4 2009
Abstract
Consumption of soybeans can reduce the risk of different types of cancer. Little is known about the effect of germination on the anticancer properties of soya. This study was done to determine if germination improves the anticancer properties of soybean protein through generation of...
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PMID: 19688264
PDF is available here.
PDF is available here.
Materiae Vegetabiles (64)4 2009
Abstract
Consumption of soybeans can reduce the risk of different types of cancer. Little is known about the effect of germination on the anticancer properties of soya. This study was done to determine if germination improves the anticancer properties of soybean protein through generation of...
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PMID: 19688264
PDF is available here.
PDF is available here.
Materiae Vegetabiles (64)4 2009
Abstract
Consumption of soybeans can reduce the risk of different types of cancer. Little is known about the effect of germination on the anticancer properties of soya. This study was done to determine if germination improves the anticancer properties of soybean protein through generation of...
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PMID: 19688264
PDF is available here.
PDF is available here.
Materiae Vegetabiles (64)4 2009
Abstract
Consumption of soybeans can reduce the risk of different types of cancer. Little is known about the effect of germination on the anticancer properties of soya. This study was done to determine if germination improves the anticancer properties of soybean protein through generation of...
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PMID: 19688264
PDF is available here.
PDF is available here.
Abstract
Diarrhea at different degrees were observed in 4% and 8% 7 S groups. The skin erythema reactions in grade "-", "+/-", "+", "++" of control group respectively were 2/4, 2/4, 0/4, 0/4, of 4% 7 S group were 0/4, 0/4, 2/4, 2/4 and of 8% 7 S group were 0/4, 0/4, 1/4, 3/4. The serum IgE and histamine leve...
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PMID: 20137559
PDF is available here.
PDF is available here.
Abstract
An in-depth analysis was carried out with expressed sequence tags (ESTs) for genes in and near the HMW-GS loci. Considerations for using ESTs are discussed, including the occurrence of chimeric and aberrant HMW-GS ESTs. Complete gene sequences demonstrated the feasibility of constructing accurate fu...
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PMID: 19767902
PDF is available here.
PDF is available here.
Abstract
Although soy proteins are often employed as functional ingredients in oil-water emulsions, very little is known about the aggregation state of the proteins in solution and whether any changes occur to soy protein dispersions during homogenization. The effect of dynamic high pressure homogenization o...
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PMID: 19415926
PDF is available here.
PDF is available here.
Abstract
BconP dose-dependently induced CCK secretion in STC-1 cells. Treatment with BAPTA-AM, an intracellular Ca2+ chelator, reduced BconP-induced CCK secretion, however, removal of extracellular Ca2+ did not affect the secretory response. Treatment with 2-amino borate (2-APB) reduced CCK releasing respons...
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PMID: 19099240
PDF is available here.
PDF is available here.
Abstract
Soybeans are recognized as one of the "big 8" food allergens. IgE antibodies from soybean-sensitive patients recognize more than 15 soybean proteins. Among these proteins only the alpha-subunit of beta-conglycinin, but not the highly homologous alpha'- and beta-subunits, has been shown to be a major...
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PMID: 19138084
PDF is available here.
PDF is available here.
Abstract
The effects of thermal treatment on the subtilisin Carlsberg-induced coagulations of soy protein isolate (SPI) and soy proteins in 7S and 11S fractions, most of which are beta-conglycinin and glycinin, respectively, were examined by measuring the turbidity (OD(660)) of the reaction solutions. With t...
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PMID: 19117398
PDF is available here.
PDF is available here.
Current Issues in Molecular Biology (11 Suppl 1) 2009
The role of green fluorescent protein (GFP) in transgenic plants to reduce gene silencing phenomena.
Abstract
The green fluorescent protein (GFP) of jellyfish (Aequorea victoria) has significant advantages over other reporter genes, because expression can be detected in living cells without any substrates. Recently, epigenetic phenomena are important to consider in plant biotechnology experiments for elucid...
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PMID: 19193961
PDF is available here.
PDF is available here.
Abstract
Three unique wheat globulin genes, Glo-3A, Glo3-B and Glo-3C, were identified. We describe the genomic structure of these genes and their expression pattern in wheat seeds. The Glo-3A gene shared 99% identity with the cDNA of WP5212 at the nucleotide and deduced amino acid level, indicating that we...
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PMID: 19615078
PDF is available here.
PDF is available here.
Abstract
We measured the total antioxidant activity (AOA). Globulin was measured to assess the inflammatory response secondary to oxidative stress. Investigations were conducted in 45 cases of recently diagnosed primary colorectal adenocarcinoma. As control, 45 age and sex matched healthy persons were chosen...
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PMID: 20509331
PDF is available here.
PDF is available here.
Liver International (28)10 2008
Abstract
Liver stiffness correlated positively with bilirubin, alkaline phosphatase (ALP), gamma-glutamyl transpeptidase (GGT), aspartate aminotransferase (AST), alanine aminotransferase (ALT), globulin, alpha-fetoprotein (AFP) and HBV DNA levels and negatively with albumin and platelet levels (P<0.05 for al...
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PMID: 18482268
PDF is available here.
PDF is available here.
Genes & Genetic Systems (83)6 2008
Abstract
We characterized the upstream region of the alpha subunit gene in terms of transcriptional control using transgenic Arabidopsis thaliana plants carrying reporter gene constructs comprising the 1357-bp upstream sequence of the alpha subunit gene and the beta-glucuronidase (GUS) gene. Analysis of the...
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PMID: 19282624
PDF is available here.
PDF is available here.
Abstract
We compared protein chemical and functional properties (thermal stability, surface hydrophobicity, solubility as a function of pH, and emulsifying properties) of these proteins from pea, fava bean, cowpea, and French bean with those of soybean as a control at the same conditions. The comparison clea...
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PMID: 18828597
PDF is available here.
PDF is available here.
Abstract
Seeds possess a high intrinsic capacity for protein production that makes them a desirable bioreactor platform for the manufacture of transgenic products. One strategy to enhance foreign protein production involves exchanging the capacity to produce intrinsic proteins for the capacity to produce a h...
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PMID: 18694455
PDF is available here.
PDF is available here.
Canadian Journal of Physiology and Pharmacology/Revue Canadienne de Physiologie et Pharmacologie (86)10 2008
Abstract
This study was conducted to examine the protective role of crude polysaccharide from brown seaweed Sargassum polycystum against acetaminophen-induced abnormality in blood glucose, serum albumin/globulin ratio, and liver glycogen, lactate, and pyruvate. Liver and renal tissue histology was performed...
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PMID: 18841170
PDF is available here.
PDF is available here.
Plant and Cell Physiology (49)10 2008
Abstract
The soybean major storage protein glycinin is encoded by five genes, which are divided into two subfamilies. Expression of A3B4 glycinin in transgenic rice seed reached about 1.5% of total seed protein, even if expressed under the control of strong endosperm-specific promoters. In contrast, expressi...
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PMID: 18776200
PDF is available here.
PDF is available here.
Abstract
We found that glycinin-induced hypersensitivity is a predominantly Th2-type immune response, mediated by IgE and associated with increases in intestinal mast cell numbers and histamine release as well as IL-4 and IL-10 concentrations in the serum of sensitized piglets, resulting in diarrhea and redu...
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PMID: 18280135
PDF is available here.
PDF is available here.
Abstract
These studies document that cytotoxicity and antibody binding can be simultaneously assessed and marks the first demonstration of complement-dependent HLA antibodies undetectable by conventional cytotoxicity. Routine application of this assay may provide new insights regarding transplantation of pat...
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PMID: 18454488
PDF is available here.
PDF is available here.
Abstract
These results show that beta-conglycinin hydrolysates play an important role in resisting S. typhimurium penetration into intestinal epithelial cells and that high molecular mass peptides (10-20 kDa) were more effective overall than low molecular mass peptides....
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PMID: 18656921
PDF is available here.
PDF is available here.
Tree Physiology (28)8 2008
Abstract
We compared the balance between deposition and degradation of storage proteins during zygotic or somatic embryogenesis and germinative growth in the two types of embryos. During mid to late zygotic embryogenesis, storage proteins accumulated and globulin 7S (GLO7A) gene transcripts were detected, wh...
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PMID: 18519247
PDF is available here.
PDF is available here.
Abstract
Our results showed that dietary supplementation of glycinin reduced piglet performance (p < 0.05), while increasing lymphocyte proliferation and CD4+/ CD8+ ratio (p < 0.01). Intestinal mucosal immune responses to glycinin were enhanced with high levels of IgA (p < 0.01) as well as interleukin-4 and...
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PMID: 18763625
PDF is available here.
PDF is available here.
Abstract
We present a novel approach that utilizes aspects of simulation and informatics in order to formulate structure-property relationships. We show how supervised learning can be utilized to overcome the sampling problem in simulation approaches. Likewise, we show how learning can be achieved based on m...
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PMID: 18672870
PDF is available here.
PDF is available here.
Transgenic Research (17)4 2008
Abstract
We have over-expressed Aspergillus niger phyA2 gene in maize seeds using a construct driven by the maize embryo-specific globulin-1 promoter. Low-copy-number transgenic lines with simple integration patterns were identified. Western-blot analysis showed that the maize-expressed phytase protein was s...
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PMID: 17932782
PDF is available here.
PDF is available here.
Abstract
We have begun to investigate its utility to understand seed development and grain filling by identifying the major seed storage proteins in a diploid accession Bd21. With the use of ID SDS-PAGE and mass spectrometry we detected seven major storage protein bands, six of which were identified as globu...
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PMID: 18528675
PDF is available here.
PDF is available here.
Abstract
A new method for the determination of free calcium concentration in human plasma was developed by online coupling capillary electrophoresis (CE) with inductively coupled plasma optical emission spectrometry (ICP-OES). Baseline separation of calcium-containing species was achieved by CE-ICP-OES in a...
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PMID: 18572926
PDF is available here.
PDF is available here.
Poultry Science (87)7 2008
Abstract
An experiment was conducted to determine the effects of different doses of liquid dl-2-hydroxy-4-methylthio butanoic acid (LMA) on growth performance and immune response in broiler chickens. In an arrangement with 4 graded levels of LMA to meet 80, 100, 120, and 140% of methionine requirements of br...
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PMID: 18577618
PDF is available here.
PDF is available here.
Abstract
Soybean is a complex matrix containing several potentially bioactive components. The objective was to develop a statistical model to predict the in vitro anticancer potential of soybean varieties based on the correlation between protein composition and bioactive components after simulated gastrointe...
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PMID: 18473471
PDF is available here.
PDF is available here.
Abstract
The recombinant proteins Adzuki 7S1, Adzuki 7S2, and Adzuki 7S3 were prepared through the Escherichia coli expression systems of three kinds of adzuki bean cDNAs. The recombinant proteins exhibited intrinsic thermal stabilities, surface hydrophobicities, and solubilities, although the homology of th...
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PMID: 18461964
PDF is available here.
PDF is available here.
Abstract
We investigated the effects of heating and digestion on the IgE binding of Ara h 3 and glycinin. Both proteins are relatively stable to denaturation, having denaturation temperatures ranging from 70 to 92 degrees C, depending on their quaternary structure and the ionic strength. Aggregates were form...
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PMID: 18338408
PDF is available here.
PDF is available here.
Journal of Food Science (73)5 2008
Abstract
Albumin and globulin fractions of 1 Desi and 2 Kabuli varieties of chickpeas (Cicer arietinum) were extracted with water and salt solutions (K(2)SO(4) and NaCl). The extractable yields and particularly the albumin-globulin ratio varied greatly with the extraction medium and chickpea variety. Dependi...
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PMID: 18576973
PDF is available here.
PDF is available here.
Abstract
Upon hydrolysis with chymotrypsin, soy glycinin has a strong tendency to aggregate. The regions of glycinin from which the aggregating peptides originate were identified by accumulative-quantitative peptide mapping. To this end, the aggregating peptides were further hydrolyzed with trypsin to obtain...
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PMID: 18461958
PDF is available here.
PDF is available here.
Abstract
The structural properties of mixtures of two molecular soy fractions, 11S (glycinin) and 2S, were investigated in the presence of glucono-delta-lactone (GDL) during an isothermal run at 25 degrees C for 1500 min. Analytical methodology included small-deformation dynamic oscillation, visual observati...
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PMID: 18341283
PDF is available here.
PDF is available here.
Abstract
We have analyzed the effect of sucrose on dynamic interfacial (dynamic surface pressure and surface dilatational properties) and foaming (foam capacity and foam stability) characteristics of soy globulins (7S and 11S). The protein (at 1 x 10(-3), 1 x 10(-2), 0.1, and 1 wt %) and sucrose (at 0, 0.25,...
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PMID: 18341284
PDF is available here.
PDF is available here.
Abstract
Our results suggest that the physiological immune depression occurring in mares, during gestation might be due to T-helper and T-cytotoxic lymphocytes reduction....
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PMID: 17986170
PDF is available here.
PDF is available here.
Bioresource Technology (99)6 2008
Abstract
Animal fleshing (ANFL), the major proteinaceous solid waste discharged from leather manufacturing industries was used as the substrate for the production of alkaline protease by Pseudomonas aeruginosa. The strain isolated from the tannery wastewater was selected for its ability to produce protease o...
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PMID: 17481889
PDF is available here.
PDF is available here.
Abstract
Abstract
OBJECTIVE: To evaluate the influence of Chinese drug Dahuang Zhechong pill on the hepatocellular function. METHOD: Thirty-seven patients with hepatocirrhosis and twelve normal controls were performed the hepatobiliary scintgraphy with Tc-99m labeled ethylene hepatobiliary iminodiacetic acid (99 mTc-...
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PMID: 18536385
PDF is available here.
PDF is available here.
Abstract
The erythrocyte sedimentation rate (ESR) can be used to identify low-grade inflammation that contributes to future vascular events. ESR determinants, however, have not been explored in the absence of a subclinical or microinflammatory response.The ESR was determined in a large cohort of apparently h...
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PMID: 18285274
PDF is available here.
PDF is available here.
Abstract
Leptin participates in modulating lipid metabolism. There are notable differences in leptin, serum lipid, and CCK between patients with gallstone and those with hepatolithiasis. The role of leptin in the pathophysiological course of cholelithiasis needs further investigation....
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PMID: 18234641
PDF is available here.
PDF is available here.
Gene families encoding 11S globulin and 2S albumin isoforms of jelly fig (Ficus awkeotsang) Achenes.
Abstract
Seed storage proteins of plants commonly comprise several groups of multiple isoforms encoded by gene families. From about 300 expressed sequence tag (EST) clones in maturing jelly fig (Ficus awkeotsang Makino) achenes, gene families encoding precursor polypeptides of two storage protein classes, in...
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PMID: 18256470
PDF is available here.
PDF is available here.
Abstract
Sensitization with soybean allergens induced high levels of antigen-specific IgE and IgG1 and increased serum histamine in BALB/c mice. Percentiles of intact mast cell of small intestine in mice sensitized with glycinin and beta-conglyinin significantly decreased for 28 days. Degranulation of mast c...
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PMID: 18070164
PDF is available here.
PDF is available here.
Abstract
We therefore compared the impact of colonization by three taxonomically different AMF species (Glomus intraradices, Glomus mosseae and Scutellospora castanea) on Medicago truncatula at the physiological and transcriptional level using quantitative-PCR. Each AMF developed a species-typical colonizati...
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PMID: 17965878
PDF is available here.
PDF is available here.
Abstract
We examined the temporal and spatial dynamics of corticosterone (CORT, baseline, and acute stress-induced) and corticosterone binding globulin (CBG) concentrations in blood of Black-legged Kittiwakes (Rissa tridactyla) breeding at four major colonies in the Bering Sea, Alaska, during 1999-2005. We f...
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PMID: 18164297
PDF is available here.
PDF is available here.
Abstract
Our results indicate that the beta-conglycinin alpha'-subunit possesses an intrinsic immune-stimulating capacity and that it can induce an allergic reaction. Moreover, this study showed that beta-conglycinin alpha'-subunit-induced anaphylaxis is IgE mediated, and mast cell degranulation and histamin...
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PMID: 17823539
PDF is available here.
PDF is available here.
Phytochemistry (69)1 2008
Abstract
We tested the hypothesis that albumin-2 fraction could be constituted by a non-processed 11S globulin (proglobulin). To this end, the gene encoding the amaranth 11S subunit was cloned and expressed in Escherichia coli. Subsequently, the recombinant proglobulin and albumin-2 purified from seeds were...
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PMID: 17714748
PDF is available here.
PDF is available here.
Atherosclerosis (196)1 2008
Abstract
We compared the effects of a soy protein isolate, a soy 7S fraction concentrate and a soy 11S fraction concentrate on total plasma cholesterol, HDL cholesterol, LDL+VLDL cholesterol and triacylglycerol in adult male cynomolgus monkeys. Effects on biomarkers of coronary risk [soluble E-selectin, vasc...
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PMID: 17350024
PDF is available here.
PDF is available here.
Abstract
Industrial soy protein isolates (SPIs) due to differences in their processing conditions may differ both in composition and in degree of hydrolysis. As a result, they display different performance in food production and final food properties like consistency and taste. To address this issue, a fast,...
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PMID: 18052236
PDF is available here.
PDF is available here.
Abstract
This article presents a novel method for immobilization of active ingredients. The method is based on CO(2) aided active ingredient co-precipitation with glycinin, a biodegradable protein matrix from edible soybean protein. Glycinin precipitates abundantly under isoelectric conditions and serves as...
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PMID: 17514752
PDF is available here.
PDF is available here.
Abstract
Commercial broilers were raised in a 5-week period and a detailed clinicochemical follow-up was carried out, to characterise a flock selected for one-sided muscle mass production. Blood samples were drawn at the ages of 1 day, 1, 2, 3, 4 and 5 weeks, and plasma enzyme activities, metabolite and ion...
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PMID: 18277704
PDF is available here.
PDF is available here.
Biomedical Chromatography (21)12 2007
Abstract
I and II), and only glycinin I underwent reversible dissociation-association system into alpha and beta species. Glycinin I and II were not found to constitute a dissociation-association system. Glycinin II also did not dissociate under varying conditions of time, pH and ionic strength of buffer. Va...
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PMID: 17604345
PDF is available here.
PDF is available here.