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Helminth Proteins (1):
Recent article
Helminth Proteins
FEBS Journal (278)18 2011
Abstract
We performed MS to identify excretory-secretory proteins from Strongyloides ratti. In the excretory-secretory proteins of the parasitic female stage, we detected, in addition to other peptides, peptides homologous with the Caenorhabditis elegans heat shock protein (HSP)-17, named Sra-HSP-17.1 (∼ 1...
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PMID: 21762402
PDF is available here.
PDF is available here.
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Abstract
Abstract
We have deduced and annotated the entire proteome of this plant-parasite to create a database of 14,420 proteins. We have made this database, termed HapPep3, available from the Superfamily repository of model organism proteomes (http://supfam.mrc-lmb.cam.ac.uk/SUPERFAMILY). To experimentally confirm...
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PMID: 20804128
PDF is available here.
PDF is available here.
Abstract
Control of Fasciola hepatica infections of livestock in the absence of vaccines depends largely on the chemical triclabendazole (TCBZ) because it is effective against immature and adult parasites. Overdependence on a single drug and improper application is considered a significant fa...
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PMID: 20726552
PDF is available here.
PDF is available here.
Abstract
Neurocysticercosis (NC), an infection of the CNS with Taenia solium metacestode, exemplifies formidable public health concerns associated with significant morbidity and mortality. The disease is a complex phenomenon involving molecular cell biological cross-talks between the parasite and human host....
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PMID: 20973642
PDF is available here.
PDF is available here.
Parasitology Research (107)5 2010
Abstract
The MYND-type zinc finger protein (MYND-ZF) is a large group of proteins containing the MYND domain which play an important role in protein-protein interactions. A cDNA clone encoding a novel MYND-ZF was isolated and identified from a Clonorchis sinensis (C. sinensis) adult cDNA library. The open re...
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PMID: 20680338
PDF is available here.
PDF is available here.
Parasitology Research (107)5 2010
Abstract
Albendazole is a benzimidazole drug which can be used to treat liver fluke (Fasciola hepatica) infections. Its mode of action is believed to be the inhibition of microtubule formation through binding to β-tubulin. However, F. hepatica expresses at least six different isotypes of β-tubulin, and thi...
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PMID: 20676683
PDF is available here.
PDF is available here.
Parasitology (137)10 2010
Abstract
We report results obtained from a proteomic analysis of the proteins from S. stercoralis L3 larvae obtained from patients. Since the genome of S. stercoralis is not yet available, we used proteomic analysis to identify 26 different proteins, 13 of them released by short digestion with trypsin, which...
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PMID: 20388238
PDF is available here.
PDF is available here.
Abstract
Whole proteins of male and female adult Haemonchus contortus were analysed by immunoproteomic techniques. Approximately 662 and 680 spots were detected on proteome maps of male and female nematodes, respectively, stained with Coomassie brilliant blue G-250. There were 609 shared spots. Approximately...
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PMID: 19560953
PDF is available here.
PDF is available here.
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (26)8 2010
Abstract
To investigate the immune responses and the protection induced by the transgenic Alfalfa (Medicago sativa) containing Eg95-EgA31 fusion gene of Echinococcus granulosus against Eg protoscoleces.
The leaf protein was extracted from the transgenic alfalfa by heat-coagulation method, its concentration w...
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PMID: 20619100
PDF is available here.
PDF is available here.
Abstract
A full-length complementary DNA (cDNA; designated Hc-stp-1) encoding a serine/threonine phosphatase (Hc-STP-1) was isolated from Haemonchus contortus, a strongylid nematode parasite of small ruminants. Hc-stp-1 was shown to be transcribed in males of both adults and fourth-stage larvae, but not in f...
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PMID: 20153820
PDF is available here.
PDF is available here.
Abstract
We identified a novel GST gene of Taenia solium metacestodes (TsMs), which is a causative agent of neurocysticercosis. The 804 bp-long cDNA encoded a 639 bp open reading frame (212 amino acid polypeptide), which exhibited the structural motif and domain organisation characteristic of GST. It formed...
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PMID: 20385139
PDF is available here.
PDF is available here.
Abstract
These results indicate a major role for SmPlk1 in parasite reproduction and suggest its importance as a potential new target against schistosomiasis.
Copyright 2010 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved....
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PMID: 20350550
PDF is available here.
PDF is available here.
Parasitology Research (107)3 2010
Abstract
Enolase is a key enzyme in the glycolytic pathway; recent studies have also shown that enolase is found on the surface of several parasites, where it acts as a plasminogen-binding protein. In the present study, the enolase of Schistosoma japonicum has been cloned and expressed. In western blot analy...
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PMID: 20512506
PDF is available here.
PDF is available here.
Parasitology Research (107)3 2010
Abstract
We describe a homologue of the Tsunagi protein from Schistosoma japonicum (SjTsunagi). The gene for this protein was isolated from S. japonicum using degenerate and anchored polymerase chain reaction (PCR), and the deduced protein has sequence homology and similarity to Tsunagi protein of other spec...
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PMID: 20467751
PDF is available here.
PDF is available here.
Abstract
We have identified a total of 20 full-length cDNA sequences corresponding to L-AChR subunits in three parasitic trichostrongylid species of which 14 correspond to novel sequences. Genes orthologous to unc-29, unc-63, unc-38 and lev-1 were found in each trichostrongylid species, whereas no gene corre...
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PMID: 20531256
PDF is available here.
PDF is available here.
Parasitology Research (107)2 2010
Abstract
Thioredoxin reductase (TrxR) is an evolutionary diverse enzyme. In higher eukaryotes, TrxR contains a selenocysteine in the active site which confers high activity and a broad substrate range. The parasitic nematode Haemonchus contortus contains two TrxRs, a cytoplasmic enzyme HcTrxR1 with a selenoc...
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PMID: 20495929
PDF is available here.
PDF is available here.
Abstract
We present a survey of R. reniformis ESTs that were sequenced from a sedentary parasitic female cDNA library. Cluster analysis of 2004 high quality ESTs produced 123 contigs and 508 singletons for a total of 631 R. reniformis unigenes. BLASTX analyses revealed that 39% of all unigenes showed similar...
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PMID: 20346373
PDF is available here.
PDF is available here.
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases (28)3 2010
Abstract
To clone and express endothelial differentiation-related factor (SjEDF)-1 gene of Schistosoma japonicum, analyze its immunogenicity and the stage-specific expression at different developmental stages of S. japonicum.
Total RNA were extracted from eggs, cercariae, schistosomula and adult worms. The h...
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PMID: 20806499
PDF is available here.
PDF is available here.
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases (28)3 2010
Abstract
To study the protective effects on the infection of Schistosoma japonicum in C57BL/6 mice induced by dendritic cells DCs pulsed with GST in combination with CpG oligodeoxynucleotide.
GST was purified and used to stimulate DC2.4 cell line. Antigen loading was analyzed by immunofluorescence method. Th...
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PMID: 20806501
PDF is available here.
PDF is available here.
Abstract
Human pulmonary and subcutaneous dirofilariosis caused by Dirofilaria immitis and Dirofilaria repens are worldwide diagnosed with increasing frequency. These species are responsible for the development of benign pulmonary and subcutaneous nodules, respectively, that can be confused with lung or cuta...
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PMID: 20197111
PDF is available here.
PDF is available here.
Abstract
We have targeted two genes from the root-knot nematode Meloidogyne incognita; a dual oxidase gene implicated in the tyrosine cross-linking of the developing cuticle and a subunit of signal peptidase, a protein complex required for the processing of secreted proteins. While these genes are involved i...
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PMID: 20100489
PDF is available here.
PDF is available here.
An ortholog of the ecdysone receptor protein (EcR) from the parasitic nematode Haemonchus contortus.
Abstract
We report the cloning of cDNA encoding an EcR ortholog (HcEcR) from H. contortus mRNA expressed during L3. Phylogenetically, this and the putative EcR from Brugia malayi form a separate branch between arthropod EcRs and liver X receptors. Two isoforms of HcEcR differ in the inclusion/omission of a 3...
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PMID: 20226216
PDF is available here.
PDF is available here.
Abstract
We still do not know how these alleles are contributing to resistance in vivo. To understand the phenomenon of anthelmintic resistance in detail we need to link changes in the genes of individual parasites with their ability to resist the effects of anthelmintic exposure. We have determined the geno...
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PMID: 20211658
PDF is available here.
PDF is available here.
Parasitology (137)5 2010
Abstract
We studied the occurrence of 8 filarial proteins and of Wolbachia endobacteria in the tumor cells. Onchocercomas from patients, untreated and treated with antibiotics and anthelminthics, were examined by immunohistology. Neoplasms were diagnosed in 112 of 3587 female and in 2 of 1570 male O. volvulu...
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PMID: 20199697
PDF is available here.
PDF is available here.
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases (28)2 2010
Abstract
DNA sequencing confirmed that the nucleotide sequence of synthesized IPSE gene was completely identical to the native one. SDS-PAGE showed that the recombinant plasmid IPSE/pET32a(+) expressed a fusion protein with an Mr 35700 after being induced by IPTG. The pure fusion protein Trx-IPSE reacted pos...
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PMID: 20666307
PDF is available here.
PDF is available here.
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases (28)2 2010
Abstract
The average weight of hydatid cysts in groups A, B, C, and D was (28.0 +/- 36.0), (41.0 +/- 33.0), (72.0 +/- 36.0) and (78.0 +/- 57.0) mg, respectively, the cyst weight of group A was lower than that of group D (P < 0.05), decreased by 64.1%. The levels of IFN-gamma, IL-12 and TNF-alpha in group A [...
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PMID: 20666308
PDF is available here.
PDF is available here.
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases (28)2 2010
Abstract
A 1211 bp of 3'-end of AsCP gene was amplified by 3'RACE, full length of the gene was 1462 bp and coding 411 amino acids. It showed 36.4% identity with the L-cysteine protease of Caenorhabditis elegans. Double enzyme digestion of the constructed recombinant plasmid pET32a(+)-AsCP showed that there w...
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PMID: 20666320
PDF is available here.
PDF is available here.
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases (28)2 2010
Abstract
A total of 33 protein bands were separated by SDS-PAGE (Mr 13,800-145,400). Four of them were high-abundance proteins with Mr 26,500, Mr 37,600, Mr 88,200, and Mr 130,200, respectively. At least two protein bands of Mr 31,600 and 37,600 reacted with the infected mice sera. 367 protein spots were det...
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PMID: 20666318
PDF is available here.
PDF is available here.
Abstract
Journal of Helminthology (84)1 2010
Abstract
Glutathione S-transferase from an Indian isolate of Fasciola gigantica of buffalo origin was isolated and characterized. Total RNA was transcribed to cDNA by reverse transcription and an amplicon of 657 bp glutathione S-transferase gene was obtained by polymerase chain reaction (PCR). The present is...
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PMID: 19619366
PDF is available here.
PDF is available here.
Journal of Helminthology (84)1 2010
Abstract
Schistosoma haematobium and S. bovis are widespread schistosome species causing human and cattle schistosomiasis, respectively, in Africa. The sympatric occurrence of these two species and their ability to infect the same Bulinus intermediate snail hosts necessitates precise methods of identificatio...
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PMID: 19646307
PDF is available here.
PDF is available here.
Parasitology (137)3 2010
Abstract
We review what is known about the process in these parasites and provide an update on the methodology and machinery of RNAi. Data are presented to demonstrate that: (1) not all schistosome genes can be suppressed to the same extent, using the methods employed here; (2) while there is variation in th...
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PMID: 19765345
PDF is available here.
PDF is available here.
Abstract
Intracolonic injection of TNBS caused severe colitis. Treatment with S. mansoni proteins significantly ameliorated colonic inflammation after 5 d. TNBS did not affect gastric emptying but significantly decreased the geometric center and impaired colonic peristaltic activity 5 d after the induction o...
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PMID: 20135718
PDF is available here.
PDF is available here.
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases (28)1 2010
Abstract
The recombinant plasmid of pGEX 4T-3/SjMRLC was constructed correctly. The recombinant protein (rSjMRLC) with GST tag was soluble. SjMRLC showed similar transcription level in all stages including sporocyst, miracidium, egg, cercaria, schistosomulum, female worm, male worm, and paired adults. CONCLU...
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PMID: 20411742
PDF is available here.
PDF is available here.
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases (28)1 2010
Abstract
The new gene Ts3 (GenBank accession No. EU338456) was obtained with 531 bp and an ORF of C. cellulosae. With relatively stable structure and physicochemical properties, the new gene was anon-transmembrane protein and contained more protein kinase C phosphorylation site. To induce its expression, the...
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PMID: 20411748
PDF is available here.
PDF is available here.
Abstract
There have been studied the activity and properties of fructose bisphosphatase (FBPase) of Bothriocephalus scorpii parasitizing in pyloric appendages of the goby Myoxocephalus brandti. All subcellular fractions of B. scorpii (12 g-cytosol, 105 g-cytosol, mitochondria, and microsomes) have the FBPase...
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PMID: 20432706
PDF is available here.
PDF is available here.
Abstract
As there appeared to be no data available on Toxocara canis infection in the children of Swaziland, a serological survey of T. canis infection was recently conducted among 92 children aged 3-12 years from rural slums in the low- and middle-veld. A child was considered seropositive if, in western blo...
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PMID: 20149294
PDF is available here.
PDF is available here.
Abstract
This study was undertaken to analyze the nucleotide sequences of a marker fragment in the mitochondrial cox1 gene in polymorphic variants of G1 strain from the nucleotide sequence bank "Genbank" and to choose conditions for a polymerase chain reaction-restriction fragment length polymorphism (PCR-RF...
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PMID: 20873372
PDF is available here.
PDF is available here.
Abstract
Our previous studies showed that recombinant Sj16 (rSj16) could suppress host's inflammatory responses and inhibit macrophage maturation. In the present study, the effects of rSj16 on the differentiation of the murine myeloid leukemia WEHI-3B JCS cell line and on mouse hematopoiesis were investigate...
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PMID: 20204135
PDF is available here.
PDF is available here.
Abstract
We describe the participation of an ERK-like protein in estrogen-dependent reproduction of the helminth parasite Taenia crassiceps. Our results show that 17beta-estradiol induces a concentration-dependent increase in the bud number of in vitro cultured cysticerci. If parasites are also incubated in...
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PMID: 20145710
PDF is available here.
PDF is available here.
Parasitology (137)1 2010
Abstract
We aimed to identify TGF-beta homologues from 2 laboratory model nematodes (Heligmosomoides polygyrus and Nippostrongylus brasiliensis) and 2 major parasites of ruminant livestock (Haemonchus contortus and Teladorsagia circumcincta). Parasite cDNA was used as a template for gene-specific PCR and RAC...
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PMID: 19712539
PDF is available here.
PDF is available here.
Abstract
We report the expression and identification of a PREB-related gene from the planarian Dugesia japonica, DjPreb. The planarian DjPreb cDNA is comprised of 1101 bp and contains a 972 bp open reading frame corresponding to a deduced protein of 323 amino acids with a 69 bp 5'-UTR and a 60 bp 3'-UTR. Phy...
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PMID: 20198854
PDF is available here.
PDF is available here.
Journal of Comparative Pathology (143)2-3 2010
Abstract
Worm burden, hepatic damage and local cellular and humoral immune responses were assessed in goats immunized with glutathione-S-transferase and challenged with Fasciola hepatica. Infected but unimmunized and uninfected control groups were also studied. Hepatic damage was evaluated grossly and micros...
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PMID: 20185148
PDF is available here.
PDF is available here.
Abstract
Genome Research (19)12 2009
Abstract
We compare temporal expression profiles of one-to-one orthologs in conserved or divergent genomic positions in two genetically distant nematode species-Caenorhabditis elegans and C. briggsae-that share a near-identical developmental program. We find, for all major patterns of temporal expression, a...
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PMID: 19745112
PDF is available here.
PDF is available here.
Abstract
We identified the gene encoding paramyosin of Clonorchis sinensis (CsPmy) and characterized biochemical and immunological properties of its recombinant protein. CsPmy showed a high level of sequence identity with paramyosin from other helminth parasites. Recombinant CsPmy (rCsPmy) expressed in bacte...
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PMID: 19967083
PDF is available here.
PDF is available here.
Abstract
We observed 62 specimens collected from Korean residents and analyzed them by morphological features and nucleotide sequences of mitochondrial cox1 gene as well as the ITS1 region. Adult tapeworms were examined after carmine or trichrome stain. Longitudinal sections of the gravid proglottids showed...
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PMID: 19967084
PDF is available here.
PDF is available here.
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases (27)6 2009
Abstract
To clone, express and characterize a tegument protein gene of Schistosoma japonicum (Sj29) , and investigate the immune protection of the recombinant protein against S. japonicum in mice.
The gene coding for Sj29 protein was amplified by PCR, and the sequence was analyzed by bioinformatics tools. Pa...
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PMID: 20232628
PDF is available here.
PDF is available here.
The Korean Journal of Parasitology (47 Suppl) 2009
Abstract
During the past several decades, researches on parasite genetics have progressed from biochemical and serodiagnostic studies to protein chemistry, molecular biology, and functional gene studies. Nowadays, bioinformatics, genomics, and proteomics approaches are being applied by Korean parasitology re...
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PMID: 19885336
PDF is available here.
PDF is available here.
Abstract
We report the isolation and functional characterization of five new CLE genes from the potato cyst nematode Globodera rostochiensis. Unlike typical plant CLE peptides that contain a single CLE motif, four of the five Gr-CLE genes encode CLE proteins with multiple CLE motifs. These Gr-CLE genes were...
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PMID: 19656047
PDF is available here.
PDF is available here.
Abstract
Dirofilaria immitis is an important filarial parasite of dogs and cats, and a useful model for human filariasis. Current diagnostic tests for heartworm infection in animals rely on the presence of fecund female worms (usually found 6.5 months post-infection or later) and therefore fail to detect pre...
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PMID: 8728997
PDF is available here.
PDF is available here.
Abstract
We examine the relationship between praziquantel (PZQ), the current drug of choice against schistosomiasis, and Pgp expression in Schistosoma mansoni. We show that levels of RNA for SMDR2, a Pgp homolog from S. mansoni, increase transiently in adult male worms following exposure to sub-lethal concen...
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PMID: 19406169
PDF is available here.
PDF is available here.
Abstract
We survey our knowledge of helminth secreted molecules, and summarise current understanding of the growing number of individual helminth mediators that have been shown to target key receptors or pathways in the mammalian immune system....
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PMID: 19406170
PDF is available here.
PDF is available here.
Abstract
We describe type 1 cystatin, a cysteine protease inhibitor, as a major released antigen of the tropical liver fluke Fasciola gigantica (FgStefin-1). Immunohistochemical analysis showed that FgStefin-1 is abundant in (a) tissue of tegumental type, including oral and ventral sucker, pharynx, genital a...
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PMID: 19416741
PDF is available here.
PDF is available here.
FASEB Journal (23)8 2009
Abstract
We have cloned and characterized. The cDNA encodes a predicted 304-aa protein (SmAQP) that is found largely in the parasite tegument by immunolocalization and is most highly expressed in the intravascular life stages. Treatment of parasites with short interfering RNAs targeting the SmAQP gene result...
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PMID: 19364765
PDF is available here.
PDF is available here.
Abstract
There were (255 +/- 10) and (224 +/- 12) spots detected for soluble proteins and (200 +/- 11) and (132 +/- 8) spots for hydrophobic proteins from adult female and male worms respectively. Six differential proteins were identified, five up-regulated proteins in female worms were thioredoxin, putative...
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PMID: 20021849
PDF is available here.
PDF is available here.
Evaluation of protective immunity of 28KD-GST as a vaccine against experimental Schistosoma mansoni.
Abstract
Schistosoma mansoni GST was purified from adult worm homogenates by affinity chromatography. SDS-PAGE analysis of the purified antigen revealed that SmGST has molecular weight around 28 KD was used as a vaccine in a dose of 25 and 35 microg. Eleven groups of BALB/c mice of 10 mice each were vaccinat...
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PMID: 19795747
PDF is available here.
PDF is available here.
Development (136)13 2009
Abstract
We show that all known MSP-dependent meiotic maturation events in the germline require Galpha(s)-adenylate cyclase signaling in the gonadal sheath cells. We show that the MSP hormone promotes the sustained actomyosin-dependent cytoplasmic streaming that drives oocyte growth. Furthermore, we demonstr...
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PMID: 19502483
PDF is available here.
PDF is available here.
Development (136)13 2009
Abstract
We show that GLP-1/Notch signaling functions in the germline to modulate oocyte growth when sperm are available for fertilization and the major sperm protein (MSP) hormone is present. Reduction-of-function mutations in glp-1 cause oocytes to grow abnormally large when MSP is present and Galpha(s)-ad...
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PMID: 19502484
PDF is available here.
PDF is available here.