Find your PDFs fast.
Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Hemeproteins (4):
Recent article
Hemeproteins
Abstract
The cellular traffic of haem during the development of the human malaria parasite Plasmodium falciparum, through the stages R (ring), T (trophozoite) and S (schizonts), was investigated within RBC (red blood cells). When Plasmodium cultures were incubated with a fluorescent haem analogue, ZnPPIX (Zn...
|
PMID: 20491653
PDF is available here.
PDF is available here.
| < More recent | Older article > |
Abstract
Our data reveal that HE induces modest inhibition of IL-12p70 responses to lipopolysaccharide (LPS) whereas Hz significantly impairs IL-12p70 responses to IFNgamma/LPS through down-regulation of IL-12p35 and p40 gene expression. Although reactive oxygen species (ROS) are generated after short-term e...
|
PMID: 20211185
PDF is available here.
PDF is available here.
Abstract
We summarize work that reveals HNO formation through oxidative heme protein metabolism of various nitrogen-containing substrates including hydroxylamine, hydroxyurea, hydroxamic acids, cyanamide, and sodium azide. Depending on the enzyme, the nascent HNO reductively nitrosylates the heme protein or...
|
PMID: 20502824
PDF is available here.
PDF is available here.
Abstract
We amplified a 4017-bp DNA fragment of streptococcal hemoprotein receptor gene from chromatosome DNA. This DNA fragment was subcloned into the pJR700 plasmid to create pXL28. Using pXL28 as the template we got the DNA fragment deleted 1831 bp in streptococcal hemoprotein receptor gene by inverse PCR...
|
PMID: 20687350
PDF is available here.
PDF is available here.
Abstract
We developed a means to study cellular heme insertion into apo-protein targets over a 3-h period and then investigated how nitric oxide (NO) released from a chemical donor (NOC-18) might influence heme (protoporphyrin IX) insertion into seven targets that present a range of protein structures, heme...
|
PMID: 20211245
PDF is available here.
PDF is available here.
Microbiology (156)Pt 6 2010
Abstract
Streptococcus equi possesses a haem-uptake system homologous to that of Streptococcus pyogenes and Streptococcus zooepidemicus. The system consists of two ligand-binding proteins (Shr and Shp) and proteins (HtsA-C) with homology to an ABC transporter. The haem-uptake system of S. equi differs from t...
|
PMID: 20223800
PDF is available here.
PDF is available here.
Biophysical Chemistry (149)1-2 2010
Abstract
We summarize our efforts towards the understanding of heme biochemistry. We present: 1) results on ligand migration on globins coupled to the ligand binding event, 2) results on the localization of the spin density in compound I of cytochromes and peroxidases, 3) novel methodologies for mapping the...
|
PMID: 20400222
PDF is available here.
PDF is available here.
Abstract
Flavohemoglobins constitute a group of proteins involved in the metabolism of nitric oxide (NO). Botrytis cinerea was shown to have a single flavohemoglobin coding gene, Bcfhg1. Its expression was developmentally regulated, with maximum expression levels during germination of conidia, and was enhanc...
|
PMID: 20223291
PDF is available here.
PDF is available here.
Abstract
We have described previously. A high-resolution (2.00 A) crystal structure revealed that the unnatural porphyrin binds to the proteins in a manner similar to the native heme and does not perturb the protein fold. The protein scaffolds were found to provide unique coordination environments for RuMP a...
|
PMID: 20373741
PDF is available here.
PDF is available here.
Abstract
Abstract
We have solved the x-ray crystal structure of MauG complexed with preMADH to 2.1 angstroms. The c-type heme irons and the nascent TTQ site are separated by long distances over which electron transfer must occur to achieve catalysis. In addition, one of the hemes has an atypical His-Tyr axial ligatio...
|
PMID: 20223990
PDF is available here.
PDF is available here.
Abstract
The growing drug resistance of Plasmodia spp. to current antimalarial agents in the quinine and artemisinin families further asserts the need for novel drug classes to combat malaria infection. One approach to the discovery of new antimalarials is the screening of natural product extracts for activi...
|
PMID: 20230372
PDF is available here.
PDF is available here.
Abstract
We investigated some of the physico-chemical and physiological aspects of heme crystallization induced by Rhodnius prolixus PMVM. Hz formation in vitro proceeded optimally at pH 4.8 and 28 degrees C, apparently involving three kinetically distinct mechanisms along this process. Furthermore, the inse...
|
PMID: 20060043
PDF is available here.
PDF is available here.
Abstract
We were successful in providing a new coexpressed system for PHB synthesis in E. coli. This coexpressed system could be regulated by arabinose inducer, and is more stable and cheaper than other induced systems (e.g. IPTG). Furthermore, it could be applied in many biotechnology or fermentation proces...
|
PMID: 19943886
PDF is available here.
PDF is available here.
Cell Host & Microbe (7)1 2010
Abstract
We find that immunization of mice with a crude blood stage extract of the malaria parasite Plasmodium falciparum elicits parasite antigen-specific immune responses via Toll-like receptor (TLR) 9 and that the malarial heme-detoxification byproduct, hemozoin (HZ), but not malarial DNA, produces a pote...
|
PMID: 20114028
PDF is available here.
PDF is available here.
Abstract
We show that both synthetic and endothelial-derived ET-1 are removed by parasitized RBC (D10 and W2 strains, chloroquine sensitive, and resistant, resp.) and native haemozoin (HZ, malaria pigment), but not by normal RBC, delipidized HZ, or synthetic beta-haematin (BH). The effect is dose dependent,...
|
PMID: 20204072
PDF is available here.
PDF is available here.
BMC Microbiology (10)1 2010
Abstract
We observed that the hbp35 gene was transcribed as a 1.1-kb mRNA with subsequent translation resulting in three proteins with molecular masses of 40, 29 and 27 kDa in the cytoplasm, and one modified form of the 40-kDa protein on the cell surface. A recombinant 40-kDa HBP35 exhibited thioredoxin acti...
|
PMID: 20500879
PDF is available here.
PDF is available here.
Biochemistry (Washington) (48)36 2009
Abstract
These results are comparable to those observed by Olea et al. for Tt H-NOX P115A in crystal form, where four different heme structures were determined from a single unit cell. On the basis of the resonance Raman spectra, it is clear that the actual heme conformation for Tt H-NOX P115A in solution is...
|
PMID: 19653642
PDF is available here.
PDF is available here.
Abstract
We report on the reduction of nitrite in the absence of a second substrate at the heme center of the ferriheme protein nitrophorin 7 (NP7) resulting in the formation of NO and restoration of the ferriheme center. The product was spectroscopically characterized, in particular by resonance Raman and F...
|
PMID: 19655755
PDF is available here.
PDF is available here.
Biochemistry (Washington) (48)34 2009
Abstract
A binding site for iron protoporphyrin IX (heme) was designed and embedded in a photosynthetic non-heme protein, phycocyanin, which forms a globin-like backbone structure, called a globin fold, but lacks sequence similarity to the globin family containing myoglobins and hemoglobins. Based on the str...
|
PMID: 19601582
PDF is available here.
PDF is available here.
Abstract
Human SOUL (hSOUL) is a 23 kDa haem-binding protein that was first identified as the PP(23) protein isolated from human full-term placentas. Here, the overexpression, purification and crystallization of hSOUL are reported. The crystals belonged to space group P6(4)22, with unit-cell parameters a = b...
|
PMID: 19574650
PDF is available here.
PDF is available here.
Biochemistry (Washington) (48)25 2009
Abstract
Our results indicate that DevS is a gas sensor in vivo rather than a redox sensor and that the stability of its ferrous-oxy complex is enhanced by interdomain interactions....
|
PMID: 19463006
PDF is available here.
PDF is available here.
Abstract
In Pseudomonas aeruginosa, flavohemoglobin (Fhp) and its cognate regulator FhpR (PA2665) form a protective regulatory circuit, which responds to reactive nitrogen species and is also capable of protecting cells against nitrosative stress. Recently, it has been shown that the expression of the fhp pr...
|
PMID: 19767835
PDF is available here.
PDF is available here.
Abstract
Rhodnius prolixus Nitrophorin 4 (abbreviated NP4) is an almost pure beta-sheet heme protein. Its dynamics is investigated by X-ray structure determination at eight different temperatures from 122 to 304 K and by means of Mössbauer spectroscopy. A comparison of this beta-sheet protein with the pur...
|
PMID: 19271215
PDF is available here.
PDF is available here.
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases (27)2 2009
Abstract
To study the characteristics of inhibition on hemozoin formation by chloroquine under in vitro condition.
Under different concentrations (0.5-2 mol/L) of sodium acetate (NaAc) and at the pH range of 4.0-5.0, chloroquine was tested for inhibition of beta-hematin (hemozion) formation by using the HPIA...
|
PMID: 19856499
PDF is available here.
PDF is available here.
Acta Tropica (109)3 2009
Abstract
Plasmodium falciparum sequesters in the placenta. Cell-Dyn® automated flow cytometric haematology analysers have the capacity to detect haemozoin-containing circulating leukocytes during routine FBC analysis. In Lambaréné, Gabon, 685 FBCs of pregnant women were analysed, yielding 86.8% sensitivity...
|
PMID: 19121281
PDF is available here.
PDF is available here.
Acta Tropica (109)3 2009
Abstract
Resistance to synthetic first-line antimalarial drugs is considered to be a major cause of increased malaria morbidity and mortality. Use of artemisinin-based combination therapies (ACTs) is being encouraged to reduce the malaria mortality in areas of falciparum resistance. Artemisinin is a natural p...
|
PMID: 19063856
PDF is available here.
PDF is available here.
Abstract
We have determined a rotamer distribution for helical histidines bound to heme cofactors. Analysis of the entire nonredundant database for helical sequence preferences near the ligand histidine demonstrated little preference for amino acid side chain identity, size, or charge. Analysis of the databa...
|
PMID: 18636480
PDF is available here.
PDF is available here.
Abstract
We have replaced the native axial heme-coordinating histidine residue with glycine, alanine, and cysteine. We report here the characterization of the cysteine mutant H60C_NP1 by spectroscopic and crystallographic methods. The UV/vis, resonance Raman, and magnetic circular dichroism spectra suggest w...
|
PMID: 18923851
PDF is available here.
PDF is available here.
Abstract
We measured the individual and combined effects of NO and GSNO on both cell growth and the formation of protein-bound NO species. Our results suggest an intracellular equilibrium between NO and GSNO, dependent in part on cell-catalyzed release of NO from GSNO (i.e., "SNO-lyase" activity). However, w...
|
PMID: 19138101
PDF is available here.
PDF is available here.
Microbes and Infection (11)8-9 2009
Abstract
Regulated upon activation, normal T-cell expressed, and secreted (RANTES, CCL-5) is an important immunoregulatory mediator that is suppressed in children with malarial anemia (MA). Although pro-inflammatory (e.g., TNF-alpha, IL-1beta and IFN-gamma) and anti-inflammatory (e.g., IL-4, IL-10 and IL-13)...
|
PMID: 19427395
PDF is available here.
PDF is available here.
Abstract
Nitric oxide (NO) functions in biology as both a critical cytotoxic agent and an essential signaling molecule. The toxicity of the diatomic gas has long been accepted; however, it was not known to be a signaling molecule until it was identified as the endothelium-derived relaxing factor (EDRF). Sinc...
|
PMID: 19089323
PDF is available here.
PDF is available here.
Abstract
We probe the chemical requirements for proper distal hydrogen bonding in a series of synthetic model compounds for dioxygen-binding heme proteins. The model compounds 1-Co to 7-Co bear different distal residues. The hydrogen bonding in their corresponding dioxygen adducts is directly measured by pul...
|
PMID: 19053091
PDF is available here.
PDF is available here.
Abstract
Artificial biolumiscence: The use of a supramolecular hydrogel to confine heme proteins and luminol can enhance the quantum yield of chemiluminescence (CL) by about an order of magnitude and give an emission time 4-6 times longer.Confining heme proteins and luminol with the nanofibers of self-assemb...
|
PMID: 19206114
PDF is available here.
PDF is available here.
Molecular and Cellular Biochemistry (321)1-2 2009
Abstract
We assessed the contribution of oxidation-induced modifications of hemoglobin and membrane protein band 3 in the reduced survival of red cells in VL. Oxidative transformation of oxyhemoglobin to hemichrome enhanced its interaction with erythrocyte membrane in the infected animals. Association betwee...
|
PMID: 18777164
PDF is available here.
PDF is available here.
Abstract
Journal of Bacteriology (191)1 2009
Abstract
We report that a novel transcriptional activator, PG1237, is required for the expression of humY and humR, but not other iron acquisition-related genes, such as fetB and tlr, which also encode hemin binding proteins. Real-time reverse transcription-PCR analysis revealed that a mutation in the pg1237...
|
PMID: 18931136
PDF is available here.
PDF is available here.
Biochemistry (Washington) (47)52 2008
Abstract
We showed that binding of ascorbate to ascorbate peroxidase occurred at the gamma-heme edge. Here, we show that the closely related cytochrome c peroxidase enzyme can duplicate the substrate binding properties of ascorbate peroxidase through the introduction of relatively modest structural changes a...
|
PMID: 19061385
PDF is available here.
PDF is available here.
Biophysical Journal (95)12 2008
Abstract
We use an effective force constant introduced by Zaccai, the resilience, to quantify this flexibility. Site-selective experimental and computational methods allow us to determine the resilience of heme protein active sites. The vibrational density of states of the heme Fe determined using nuclear re...
|
PMID: 18835904
PDF is available here.
PDF is available here.
Abstract
We used the software GRAMM, HEX, and MACRODOX to build the protein complex with further evaluation by GROMACS potential. After molecular mechanics refinement by GROMACS the protein complexes were evaluated in terms of the following criteria: Hydrophobic packing, proximity of the hemes, hydrogen bond...
|
PMID: 18808199
PDF is available here.
PDF is available here.
Biochemical Society transactions (36)Pt 6 2008
Abstract
Our knowledge of the overall haem-scavenging mechanism on the bacterial surface is limited. A detailed description of the haem-binding properties in the transport pathway is critical to our understanding of the mechanism for haem-iron scavenging in S. aureus. Our work involves using a combination of...
|
PMID: 19021512
PDF is available here.
PDF is available here.
Abstract
A novel series of 4-aminoquinoline-containing 2-imidazolines were synthesized via a one-pot 3-component condensation reaction of amine, aldehyde and isocyanoacetate. The products were obtained in high yield as well as purity and were evaluated directly against two strains of Plasmodium falciparum an...
|
PMID: 19005606
PDF is available here.
PDF is available here.
Abstract
Proteins in the H-NOX family act as sensors of NO or O2. This family includes soluble guanylate cyclase (sGC), the NO sensor that is responsible for vasodilation and neurotransmission in mammals. The crystal structures of bacterial H-NOX domains have revealed a highly distorted heme cofactor. This d...
|
PMID: 19032089
PDF is available here.
PDF is available here.
Abstract
These results show a clear link between the heme conformation and Tt H-NOX structure and demonstrate that heme distortion is an important determinant for maintaining biochemical properties in H-NOX proteins....
|
PMID: 19032091
PDF is available here.
PDF is available here.
Inorganic Chemistry (47)22 2008
Abstract
The iron complex of oxypyriporphyrin, a porphyrinoid containing a keto-substituted pyridine, was coupled with apomyoglobin. The reconstituted ferric myoglobin was found to be five-coordinate without iron-bound water molecules. The anionic ligands such as CN (-) and N 3 (-) bound the myoglobin with h...
|
PMID: 18844346
PDF is available here.
PDF is available here.
Abstract
Hemes are often incorporated into designed proteins. The importance of the heme ligand type and its orientation is still a matter of debate. Here, heme ligands and ligand orientation were investigated using a nonredundant (87 structures) and a redundant (1503 structures) set of structures to compare...
|
PMID: 18491383
PDF is available here.
PDF is available here.
Chemical Reviews (108)11 2008
Abstract
Abstract
Our findings suggest a higher prevalence of ferroportin Q248H with greater alcohol consumption, and this higher prevalence raises the possibility that the allele might ameliorate the toxicity of alcohol. Our results suggest that alcohol but not dietary iron contributes to higher body iron stores in...
|
PMID: 18782341
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1784)11 2008
Abstract
This article focuses on the application of high pressure laser flash photolysis for studies on selected hemoprotein reactions with the objective to establish details of the underlying reaction mechanisms. In this context, particular attention is given to the reactions of small molecules such as diox...
|
PMID: 18778796
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (283)42 2008
Abstract
We report experimental results that provide the first complete challenge of a proposed model for heme acquisition by Staphylococcus aureus via the Isd pathway first put forth by Mazmanian, S. K., Skaar, E. P., Gaspar, A. H., Humayun, M., Gornicki, P., Jelenska, J., Joachmiak, A., Missiakas, D. M., a...
|
PMID: 18676371
PDF is available here.
PDF is available here.
Biochemical Society Transactions (36)Pt 5 2008
Abstract
Journal of Bacteriology (190)20 2008
Abstract
We validated as direct targets eight new genes. Interestingly, the adjacent but divergently oriented fixK(1) and cycS genes shared the same FixK(2) box for the activation of transcription in both directions. This recognition site may also be a direct target for the FixK(1) protein, because activatio...
|
PMID: 18689489
PDF is available here.
PDF is available here.
Biochemistry (Washington) (47)39 2008
Abstract
The spectrum of the ferric heme domain of the direct oxygen sensor protein from Escherichia coli ( EcDosH) has been measured between pH 3.0 and 12.6. EcDosH undergoes acid denaturation with an apparent p K a of 4.24 +/- 0.05 and a Hill coefficient of 3.1 +/- 0.6 and reversible alkaline denaturation...
|
PMID: 18771281
PDF is available here.
PDF is available here.
Abstract
We propose a new mechanism for dioxygen activation in these heme systems. The mechanism involves (a) direct electrophilic addition of the Fe(II)-bound oxygen to the C2 or C3 position of the indole in a closed-shell singlet state or (b) direct radical addition of the Fe(III)-superoxide to the C2 posi...
|
PMID: 18712870
PDF is available here.
PDF is available here.
Abstract
The stresses of transportation, weaning and commingling are associated with an increased incidence of bacterial and viral pneumonia in cattle. Proteins expressed in the epithelial lining fluid (ELF) of the lungs, in conjunction with resident leukocytes, represent the first line of defence against op...
|
PMID: 18565593
PDF is available here.
PDF is available here.
Abstract
The chemical reactivity, toxicology, and pharmacological responses to nitroxyl (HNO) are often distinctly different from those of nitric oxide (NO). The discovery that HNO donors may have pharmacological utility for treatment of cardiovascular disorders such as heart failure and ischemia reperfusion...
|
PMID: 18503778
PDF is available here.
PDF is available here.
Abstract
The ubiquitous gas, carbon monoxide (CO), is of substantial biological importance, but apart from its affinity for reduced transition metals, particularly heme-iron, it is surprisingly nonreactive-as is the ferrous-carbonyl-in living systems. CO does form strong complexes with heme proteins for whic...
|
PMID: 18549826
PDF is available here.
PDF is available here.
Abstract
The affinity of SGMIB-MRT binding to A431 cells, determined by Scatchard analysis, was 22 nM, comparable to that measured before labeling. The binding of SGMIB-MRT and its internalization by A431 cancer cells was 96% and 99% EGFR specific, respectively. Paired label assays demonstrated that compared...
|
PMID: 18722270
PDF is available here.
PDF is available here.
Biochemistry (Washington) (47)34 2008
Abstract
Spectroscopic characterization of the newly discovered heme-PAS domain sensor protein BxRcoM-2 reveals that this protein undergoes redox-dependent ligand switching and CO- and NO-induced ligand displacement. The aerobic bacterium Burkholderia xenovorans expresses two homologous heme-containing prote...
|
PMID: 18672900
PDF is available here.
PDF is available here.
Abstract
Data indicated that 25.5% of carbon sources were consumed by HMP pathway, resulting in a conversion rate of 33.9% to L-threonine with a 5% dissolved oxygen concentration. With dissolved oxygen concentration of 20%, 58.08% carbon resources entered HMP pathway, giving rise to a 46.5% conversion rate....
|
PMID: 18956755
PDF is available here.
PDF is available here.