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Immobilized Proteins (2):
Recent article
Immobilized Proteins
Abstract
We report a microfluidic chip-based hydrodynamic focusing approach that minimizes sample volume for the analysis of cell-surface interactions under controlled fluid-shear conditions. Assays of statistically meaningful numbers of translocating platelets interacting with immobilized von Willebrand fac...
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PMID: 21342809
PDF is available here.
PDF is available here.
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Abstract
We will focus on the system level or global analysis of biological systems using protein microarrays. Four major types of protein microarrays will be discussed: proteome microarrays, antibody microarrays, reverse-phase protein arrays, and lectin microarrays. We will also discuss the challenges and f...
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PMID: 21257623
PDF is available here.
PDF is available here.
Abstract
We find useful for Biacore-based studies. Many kinetic-based screening assays require rigorous sample preparation and purification prior to analysis. To highlight these procedures, this protocol describes the kinetic characterisation of single chain Fv (scFv) antibody fragments from crude bacterial...
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PMID: 20978978
PDF is available here.
PDF is available here.
Abstract
Major difficulties in phosphoprotein analysis relate to the presence of a huge number of nonphosphorylated proteins and to the wide concentration dynamic range among them. In order to overcome the analysis complexity, specific clean-up and highly efficient enrichment procedures are mandatory prior t...
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PMID: 20978974
PDF is available here.
PDF is available here.
Abstract
We describe efficient procedures for the isolation of highly purified His-tagged target proteins from an Escherichia coli host using IMAC....
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PMID: 20978973
PDF is available here.
PDF is available here.
Abstract
This article defines protein stability, emphasizes its importance and surveys some notable recent publications (2004-2008) in the field of protein stability/stabilization. Knowledge of the factors stabilizing proteins has emerged from denaturation studies and from study of thermophilic (and other ex...
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PMID: 20978963
PDF is available here.
PDF is available here.
Abstract
We propose that angiogenic growth factors covalently immobilized on scaffolds for tissue engineering can be used to accomplish this goal. The main objectives of this work were: (a) to derive desirable experimental conditions for the covalent immobilization of vascular endothelial growth factor (VEGF...
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PMID: 20717888
PDF is available here.
PDF is available here.
Abstract
The preparation and characterization of heparin-immobilized microspheres which were used to bind acidic fibroblast growth factor (aFGF), vascular endothelial growth factor (VEGF), monocyte chemoattractant protein 1 (MCP-1/CCL2), and regulation upon activation normal T cell express se...
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PMID: 20827464
PDF is available here.
PDF is available here.
Acta Biomaterialia (7)1 2011
Abstract
Cardiac tissue engineering aims to regenerate damaged myocardial tissues by applying heart patches created in vitro. The present study was undertaken to explore the possible role of matrix-attached RGD peptide in the engineering of cardiac tissue within macroporous scaffolds. Neonata...
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PMID: 20688198
PDF is available here.
PDF is available here.
Acta Biomaterialia (7)1 2011
Abstract
I collagen mineralization model. In the presence of a polyacrylic acid-containing mineralization medium optimal intrafibrillar mineralization of the EDC-cross-linked collagen was achieved using 500 and 1000 μg ml⁻¹ PVPA. The mineralized fibrils exhibited a hierarchical order of intrafibrillar mi...
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PMID: 20688200
PDF is available here.
PDF is available here.
Acta Biomaterialia (7)1 2011
Abstract
The field of tissue engineering is severely limited by a lack of microvascularization in tissue engineered constructs. Biomimetic poly(ethylene glycol) hydrogels containing covalently immobilized platelet-derived growth factor BB (PDGF-BB) were developed to promote angiogenesis. Poly...
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PMID: 20801242
PDF is available here.
PDF is available here.
Acta Biomaterialia (7)1 2011
Abstract
Electrospun poly(lactic acid) (PLLA) nanofibers (NF) were modified with cationized gelatin (CG) to improve their compatibility with chondrocytes and to show in vitro and in vivo the potential applications of CG-grafted PLLA nanofibrous membranes (CG-PLLA NFM) as a cartilage tissue en...
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PMID: 20728584
PDF is available here.
PDF is available here.
Abstract
Although most in vitro studies indicate that transforming growth factor β3 (TGF-β3) immobilized scaffold is suitable for cartilage tissue engineering, in vivo studies of implanting immobilized scaffold for chondral defect repair are still lacking. This study is to evaluate the pote...
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PMID: 20872747
PDF is available here.
PDF is available here.
Biomaterials (31)34 2010
Abstract
We aimed to develop a bioartificial pancreas that can realize xeno-islet transplantation. The islets were encapsulated in agarose microbeads carrying the soluble domain of human complement receptor 1 (sCR1), which is an effective inhibitor of the classical and alternative complement activation pathw...
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PMID: 20739058
PDF is available here.
PDF is available here.
Biomaterials (31)34 2010
Abstract
The long-term success of orthopedic implants may be compromised by defective osseointegration and bacterial infection. An effective approach to minimize implant failure would be to modify the surface of the implant to make it habitable for bone-forming cells and anti-infective at the same time. In t...
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PMID: 20800276
PDF is available here.
PDF is available here.
Macromolecular Bioscience (10)11 2010
Abstract
We focus on biomaterial scaffolds designed to mediate the development and maturation of vascular networks, by immobilized growth factors. The interactive effects of multiple vasculogenic factors are discussed in the context of cardiac tissue engineering....
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PMID: 20857391
PDF is available here.
PDF is available here.
Abstract
Ti-based biomaterial has been widely used as dental and bone implant material although its bioactivity still needs improvement especially for dental implant. The bone morphogenetic proteins(BMPs) bound to Ti-based materials will attract the mesenchymal stem cells to differentiate int...
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PMID: 20957023
PDF is available here.
PDF is available here.
Applied Optics (49)28 2010
Abstract
We developed a method for immobilization of biomolecules onto thiol functionalized surfaces according to UV diffraction patterns. UV light-assisted molecular immobilization proceeds through the formation of free, reactive thiol groups that can bind covalently to thiol reactive surfaces. We demonstra...
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PMID: 20885470
PDF is available here.
PDF is available here.
Nanotechnology (21)34 2010
Abstract
We investigated the covalent biofunctionalization of magnetic nanoparticles based on carbodiimide activation. An important aspect in the covalent biofunctionalization of nanoparticles has been neglected, namely pre-concentration. Exploiting the electrostatic attraction forces between a protein and t...
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PMID: 20683138
PDF is available here.
PDF is available here.
Abstract
We describe the development and characterization of an aptamer-based method for parallel protein analyses that relies on recognition of the target protein by two unique aptamers targeting different epitopes on the protein. Our results show that the technique achieved simultaneous and quantitative de...
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PMID: 20547050
PDF is available here.
PDF is available here.
Abstract
Composite nanofiber mats (HA/TiO2) consisting of hydroxyapatite (HA) and titania (TiO2) were fabricated via an electrospinning technique and then collagen (type I) was immobilized on the surface of the HA/TiO2 composite nanofiber mat to improve tissue compatibility. The structure and...
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PMID: 20574975
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (285)30 2010
Abstract
We demonstrate that CagL, a 26-kDa protein of the gastric pathogen and type I carcinogen Helicobacter pylori, mimics fibronectin in various cellular functions. Like fibronectin, CagL contains a RGD motif and is located on the surface of the bacterial type IV secretion pili as previously shown. CagL...
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PMID: 20507990
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (285)29 2010
Abstract
We and others previously demonstrated that lung fibroblast expression of Thy-1 prevents lung fibrosis. The mechanisms underlying the anti-fibrotic effect of Thy-1 are not well understood. In this study, we showed that Thy-1 interacts with integrin alpha(v)beta(5), both in a cell-free system and on t...
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PMID: 20463011
PDF is available here.
PDF is available here.
Abstract
We demonstrate the formation of nano-thin, poly(ethylene glycol) (PEG)-rich functional conformal coatings on individual islets via layer-by-layer assembly technique. The surface of the islets is modified with biotin-PEG-N-hydroxysuccinimide (NHS), and the islets are further covered by streptavidin (...
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PMID: 20163204
PDF is available here.
PDF is available here.
Nanotechnology (21)24 2010
Abstract
We show that SiNWs can be chemically functionalized with Ni:NTA motifs, suitable for the specific immobilization of proteins via a short polyhistidine tag (His-tag) at close proximity to the SiNW surface. We demonstrate that the proteins preserve their function upon immobilization onto SiNWs. Import...
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PMID: 20498527
PDF is available here.
PDF is available here.
Abstract
Burkholderia cepacia lipase was immobilized on various types of phenyl-functionalized mesoporous silicates (MPS). MPS, anchored with a phenyl group on the silica wall and with three dimensional (3D) mesoporosity, showed highest lipase adsorption capacity and best activities both in aqueous and organ...
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PMID: 20471602
PDF is available here.
PDF is available here.
Abstract
We characterized polystyrene-binding peptides (PS-tags) that possess a specific binding affinity for hydrophilic polystyrene (phi-PS) plates. Both the FITC-labeled PS19-1 (RAFIASRRIKRP) and PS19-6 (RIIIRRIRR) peptides showed strong binding affinity for commercially available hydrophilic, but not hyd...
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PMID: 20471598
PDF is available here.
PDF is available here.
Abstract
In situ gene therapy with granulocyte-macrophage colony-stimulating factor (GM-CSF) was demonstrated to successfully inhibit tumour cell growth in a mouse orthotopic bladder cancer model, but suffered from several disadvantages, such as limited efficiency for gene delivery, low expre...
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PMID: 19627402
PDF is available here.
PDF is available here.
Abstract
We compared the biological properties of chitosan, in which the surface was enriched with fibronectin (Fn), using two methodologies: chemical immobilization, using a water-soluble carbodiimide; and simple adsorption. X-ray photoelectron spectroscopy studies confirmed the successful immobilization of...
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PMID: 20049746
PDF is available here.
PDF is available here.
Abstract
We have used the orthogonal carbodiimide condensation and copper-catalyzed azide-alkyne "click" cycloaddition (CuAAC) reactions to prepare self-assembled monolayers that present distinct peptides to stem cells in a bioinert background. The approach involved first forming mixed SAMs with three compon...
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PMID: 20353153
PDF is available here.
PDF is available here.
Abstract
Many biosensor applications are based on streptavidin (SA) binding to partially biotinylated self-assembled thiol monolayers (SAMs). In our study, binary SAMs on gold were prepared from solutions containing 16-mercapto-1-hexadecanol (thiol I) and N-(8-biotinyl-3,6-dioxa-octanamidyl)-16-mercaptohexad...
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PMID: 20158172
PDF is available here.
PDF is available here.
Abstract
We report on the use of near-edge X-ray absorption fine structure spectroscopy (NEXAFS) to characterize a widely employed immobilization chemistry that leads to the covalent attachment of a biologically relevant oligopeptide to a surface. The oligopeptide used in this study is a kinase substrate of...
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PMID: 20387822
PDF is available here.
PDF is available here.
Abstract
We have quantitatively demonstrated that chemically immobilized peptides on polymers adopt a more ordered orientation than less tightly bound physically adsorbed peptides. These differences were also observed in different chemical environments, for example, air versus water. Although numerous studie...
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PMID: 19961170
PDF is available here.
PDF is available here.
Abstract
The OraQuick HCV Rapid Antibody Test appears suitable as an aid in the diagnosis of HCV infection.
Copyright 2010 Elsevier B.V. All rights reserved....
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PMID: 20362493
PDF is available here.
PDF is available here.
Analyst (Cambridge UK) (135)5 2010
Abstract
We developed a novel assay system using an array-based spectral surface plasmon resonance (SPR) biosensor for a high-throughput analysis of matrix metalloproteinase (MMP)-3 activity. Gelatin arrays were fabricated by immobilizing gelatin, a MMP-3 substrate, on amine-modified gold arrays. MMP-3 activ...
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PMID: 20419256
PDF is available here.
PDF is available here.
Abstract
A novel, biocompatible sensing strategy based on graphene and chitosan composite film for immobilizing the hemoglobin protein was firstly adopted. The direct electron transfer and bioelectrocatalytic activity of hemoglobin after incorporation into the composite film were investigated. A pair of reve...
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PMID: 20188928
PDF is available here.
PDF is available here.
Abstract
Hydrophilic ionic liquid-polyvinyl chloride (PVC) hybrids were prepared by immobilizing N-methylimidazole (N-mim) to PVC chains in toluene. The NmimCl-PVC hybrids were characterized by FT-IR, (1)H NMR, surface charge analysis and elemental analysis. The immobilization ratio, i.e., the percentage of...
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PMID: 20188975
PDF is available here.
PDF is available here.
Chemical Communications (46)15 2010
Abstract
Photosensing performance of a system composed of photosystem I (PSI), vitamin K(1) (VK(1))-like molecular wire, and gold nanoparticles (AuNPs) in an aqueous solution was increased considerably by the addition of double surfactants, hexylamine and dodecylbenzenesulfonate.
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PMID: 20449307
PDF is available here.
PDF is available here.
Biophysical Journal (98)8 2010
Abstract
We quantified an impressive hierarchy of long-lived, high-strength states of the integrin bond, which start from basal levels with integrin activation in solutions of divalent cations and shift dramatically upward to hyperactivated states with cell signaling in leukocytes. Taking advantage of very r...
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PMID: 20409464
PDF is available here.
PDF is available here.
Abstract
We report a simple one-step UV lithography method to activate inert hydrocarbon monolayers for spontaneous formation of water and covalently immobilized protein micropatterns on the surface. Two types of hydrocarbon monolayers including octadecyltrichlorosilane (OTS) on silicon oxide and 1-hexadecen...
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PMID: 20060543
PDF is available here.
PDF is available here.
Abstract
In this paper a carbon ionic liquid electrode (CILE) was fabricated by using ionic liquid 1-ethyl-3-methylimidazolium ethylsulfate ([EMIM]EtOSO(3)) as modifier and further gold nanoparticles were in situ electrodeposited on the surface of CILE. The fabricated Au/CILE was used as a new platform for t...
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PMID: 20152469
PDF is available here.
PDF is available here.
Methods in Molecular Biology (627)627 2010
Abstract
We have designed a novel high-throughput (HTP) kinase assay using an array-based surface plasmon resonance (SPR) apparatus. For high flexibility and performance, the kinase assay procedure is divided into an in vitro phosphorylation part and a phospho-detection part on a sensor chip. Not only biotin...
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PMID: 20217618
PDF is available here.
PDF is available here.
Methods in Molecular Biology (627)627 2010
Abstract
We here show how dark-adapted rhodopsin can be immobilized on the sensor chip surface. A laser device implemented in the SPR system allowed us to trigger light-induced conformational changes in rhodopsin and to monitor light-dependent binding of the photoreceptor cell G-protein transducin to rhodops...
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PMID: 20217627
PDF is available here.
PDF is available here.
Methods in Molecular Biology (627)627 2010
Abstract
Prion diseases, also called transmissible spongiform encephalopathies (TSEs), are a group of neurodegenerative disorders affecting animals and humans. No effective treatments are currently available for the diseases, vCJD in particular. It is believed that the formation of protease-resistant insolub...
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PMID: 20217619
PDF is available here.
PDF is available here.
Methods in Molecular Biology (627)627 2010
Abstract
Surface analytical tools as surface plasmon resonance (SPR) have become increasingly important in biomedical research since they offer high detection sensitivity compared to traditional biomedical methods. For the use of SPR as a biomedical research tool there is a need to immobilize the reactants t...
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PMID: 20217626
PDF is available here.
PDF is available here.
Methods in Molecular Biology (627)627 2010
Abstract
We obtained best and reproducible results when the lectin was immobilized and its ligands were used as soluble analytes. With heterogeneous glycoconjugates such as neoglycoproteins (which are heterogeneous in terms of nature, number, and position of sugar residues) or a mixture of oligosaccharides,...
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PMID: 20217620
PDF is available here.
PDF is available here.
Journal of Biotechnology (145)4 2010
Abstract
We describe an efficient system for one-step purification and immobilization of recombinant proteins using tamavidin 2 as an affinity tag. A bacterial sialyltransferase and soybean agglutinin were fused to tamavidin 2 and expressed in E. coli and tobacco BY-2 cells, respectively. High-level expressi...
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PMID: 20026208
PDF is available here.
PDF is available here.
Immobilized kidney 28-kDa endostatin-related (KES28kDa) fragment promotes endothelial cell survival.
Abstract
The soluble KES28kDa inhibited endothelial cell proliferation: 25 versus 12.5 microg (p < 0.05); 12.5 versus 3.15 microg (p < 0.05). Immobilization of KES28kDa supports endothelial cell survival over the control (p = 0.021). Human umbilical vein endothelial cells plated on immobilized KES28kDa showe...
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PMID: 20110665
PDF is available here.
PDF is available here.
Abstract
We report 3 severe cases of drug-induced interstitial lung disease (DI-ILD) that were treated with PMX-DHP. Two DI-ILD cases were caused by methotrexate, and the third case was caused by the herbal medicine sanmotsu-ogon-to. The arterial oxygen tension/inspiratory oxygen fraction ratio improved duri...
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PMID: 20046003
PDF is available here.
PDF is available here.
Analytical Sciences (26)7 2010
Abstract
Liposomes prepared with biotinylated phospholipids and luminol entrapped were shown to be of 187 nm in size, 59% of which were unilamellar and with 43% luminol trapping efficiency. Liposome prepared from biotinylated phospholipids with a longer hydrophilic PEG2000 spacer, but not with the shorter hy...
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PMID: 20631437
PDF is available here.
PDF is available here.
Abstract
We evaluated the efficacy of transforming growth factor (TGF)-beta-immobilized magnetic beads for chondrogenesis in vitro using a mesenchymal stem cell (MSC) delivery system and an external magnetic force (EMF). MSCs isolated from the bone marrow of Sprague Dawley rats were mixed with carboxyl group...
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PMID: 19172619
PDF is available here.
PDF is available here.
Abstract
The kinetics, energetics, and structure of Cyclolinopeptide A binding with Human Serum Albumin were investigated with surface plasmon resonance and circular dichroism. The complex is formed through slow recognition kinetics that is temperature sensitive in the range of 20°C-37°C. T...
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PMID: 21436992
PDF is available here.
PDF is available here.
ASAIO Journal (56)1 2010
Abstract
We were able to significantly reduce concentrations of the proinflammatory cytokine tumor necrosis factor (TNF)-alpha from initially very high levels. Passage of blood over nonheparinized beads did not affect the TNF levels. Meanwhile, concentrations of the regulated on activation, normal T-cells ex...
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PMID: 20071911
PDF is available here.
PDF is available here.
ASAIO Journal (56)4 2010
Abstract
Abstract
We describe a robust method to covalently immobilize His(6) fusion proteins on the sensor surface for SPR analysis....
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PMID: 20217615
PDF is available here.
PDF is available here.
Abstract
Surface plasmon resonance (SPR) is a highly sensitive method for the detection of molecular interactions. One interacting partner is immobilized on the sensor chip surface while the other is injected across the sensor surface. This chapter focuses on high-affinity immobilization of protein substrate...
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PMID: 20217614
PDF is available here.
PDF is available here.
Abstract
We describe here how to construct a large human synthetic single-chain Fv (scFv) antibody library displayed on phages, where in vivo-formed complementarity-determining regions (CDRs) are shuffled combinatorially onto germline-derived human variable-region frameworks....
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PMID: 19252839
PDF is available here.
PDF is available here.
Abstract
Integration of a hydrogel and polydimethylsiloxane (PDMS)-based microfluidic device can greatly reduce the cost of developing channel-based devices. However, there are technical difficulties including the hydrophobic and inert surface properties associated with PDMS as well as back pressure and frag...
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PMID: 19722534
PDF is available here.
PDF is available here.
Analytical Biochemistry (395)1 2009
Abstract
This article reports the first electrochemical characterization of pain biomarkers that include arachidonic acid (AA), prostaglandin G(2) (PGG(2)), and cyclooxygenase 2 (COX-2). These biomarkers are mediators of pathophysiology of pain, inflammation, and cell proliferation in cancer. The article als...
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PMID: 19646944
PDF is available here.
PDF is available here.
Abstract
A highly stable biological film was prepared by casting an aqueous dispersion of protein and composite hydrogel obtained from the polysaccharide Scleroglucan (Sclg) and borax as a cross-linking agent. Heme proteins, such as hemoglobin (Hb), myoglobin (Mb), and horseradish peroxidase (HRP), were chos...
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PMID: 19694483
PDF is available here.
PDF is available here.