Find your PDFs fast.
Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Luminescent Proteins (3):
Recent article
Luminescent Proteins
Abstract
We suggest that both changes contribute to the observed fatigue. A likely cause of the decline in tetanic myoplasmic calcium is precipitation of calcium phosphate in the SR....
|
PMID: 21512148
PDF is available here.
PDF is available here.
| < More recent | Older article > |
Nature Immunology (12)4 2011
Abstract
We report that CD4+ T cell help in the form of IL-2 is required for IL-10 production by CTLs, but not for the induction of CTL effector cytokines. We show that IL-2 derived from CD4+ helper T cells cooperates with innate immune cell-derived IL-27 to amplify IL-10 production by CTLs through a Blimp-1...
|
PMID: 21297642
PDF is available here.
PDF is available here.
Abstract
We describe a method for recording synaptic currents and potentials from identified neurons in nearly intact worms. Dissection and exposure of postsynaptic neurons is facilitated by microfabricated agar substrates, and ChannelRhodopsin-2 is used to stimulate presynaptic neurons. We used the method t...
|
PMID: 21556060
PDF is available here.
PDF is available here.
Abstract
We describe a simple fluorescence microscopy approach to assess autophagy. For clarity we restrict our description here to show how the approach can be used to monitor mitophagy in yeast cells. The assay makes use of a fluorescent reporter, Rosella, which is a dual-emission biosensor comprising a re...
|
PMID: 21788936
PDF is available here.
PDF is available here.
Abstract
We here investigated the contribution of ZFPM1 to human Th1/Th2 differentiation.
The cDNA of ZFPM1 was cloned and introduced into human cord blood CD4+ T cells by a lentiviral transduction system. Then, the expression of IL-4 and IFN-γ mRNA was determined by quantitative real-time R...
|
PMID: 21646796
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (285)52 2010
Abstract
We present a spatial structure of a complex of the Ca(2+)-regulated photoprotein clytin with its green-fluorescent protein (cgGFP) from the jellyfish Clytia gregaria, and show that it accounts for the bioluminescence properties of this system in vitro. We adopted an indirect approach of combining x-...
|
PMID: 20926380
PDF is available here.
PDF is available here.
Abstract
We report the development of a suite of six integrative vectors for construction of single copy transcriptional fusions with the gfpmut3, cfp and iyfp reporter genes in Bacillus subtilis. The promoter fusions are constructed using the highly efficient ligation-independent cloning (LIC) technique mak...
|
PMID: 20600285
PDF is available here.
PDF is available here.
Abstract
We searched for proteins physically interacting with the C-terminal region of kAE1 (Ct-kAE1), which contains motifs crucial for intracellular trafficking, by a yeast two-hybrid (Y2H) system. An adaptor-related protein complex 1 μ1A (AP-1 mu1A) subunit was found to interact with Ct-kAE1. The interac...
|
PMID: 20833140
PDF is available here.
PDF is available here.
Abstract
Photoprotein obelin, the enzyme-substrate complex of polypeptide with 2-hydroperoxycoelenterazine, is responsible for bioluminescence of marine hydroid Obelia longissima. Addition of Ca(2+) to the photoprotein triggers the bioluminescent reaction with light emission. The product of the bioluminescen...
|
PMID: 20678944
PDF is available here.
PDF is available here.
Biology of the Cell (102)9 2010
Abstract
We introduce an inducible system for detecting and tracking live cell fusion products in vitro and potentially in vivo. This system is based on BiFC (bimolecular fluorescence complementation) analysis. In this approach, two proteins that can interact with each other are joined to fragments of a fluo...
|
PMID: 20590528
PDF is available here.
PDF is available here.
Abstract
We directly investigated the effects of Brn-4 on the neuronal differentiation and development of NSCs derived from the E13 rat midbrain. We found that Brn-4 knockdown in NSCs resulted in a significant decrease of MAP-2-positive neurons with immature morphology. Overexpression of Brn-4 in NSCs marked...
|
PMID: 20524937
PDF is available here.
PDF is available here.
Biophysical Journal (99)4 2010
Abstract
We describe a novel method for analyzing a three-color (ABC) FRET system called three-color spectral FRET (3sFRET) microscopy, which is fully corrected for spectral bleedthrough. The approach quantifies FRET signals and calculates the apparent energy transfer efficiencies (Es). The method was valida...
|
PMID: 20713013
PDF is available here.
PDF is available here.
Abstract
We carried out quantitative system-wide analyses of protein and mRNA expression in individual cells with single-molecule sensitivity using a newly constructed yellow fluorescent protein fusion library for Escherichia coli. We found that almost all protein number distributions can be described by the...
|
PMID: 20671182
PDF is available here.
PDF is available here.
Current Genetics (56)4 2010
Abstract
We have developed an improved and highly reproducible Agrobacterium tumefaciens-mediated transformation protocol for A. rabiei. Transformation events were confirmed through Southern hybridizations that suggest single-copy integration of reporter genes in majority of the transformants. High level exp...
|
PMID: 20461519
PDF is available here.
PDF is available here.
Abstract
We report that young migrating neurons actively control the formation and maintenance of their own migration route. New neurons secrete the diffusible protein Slit1, whose receptor, Robo, is expressed on astrocytes. We show that the Slit-Robo pathway is required for morphologic and organizational ch...
|
PMID: 20670830
PDF is available here.
PDF is available here.
Abstract
We determine the lineages of adult hematopoietic cells derived from Tie2-expressing populations using Tie2-Cre;Rosa26R-EYFP mice. In Tie2-Cre;Rosa26R-EYFP mice, analysis of bone marrow cells showed Cre-mediated recombination in 85% of the population. In adult bone marrow and spleen, we analyzed subc...
|
PMID: 20645309
PDF is available here.
PDF is available here.
Abstract
We report that DISC1 is regulated during embryonic neural progenitor proliferation and neuronal migration through an interaction with DIX domain containing-1 (Dixdc1), the third mammalian gene discovered to contain a Disheveled-Axin (DIX) domain. We determined that Dixdc1 functionally interacts with...
|
PMID: 20624590
PDF is available here.
PDF is available here.
Abstract
Starting with the first publication of lacZ gene fusion in 1980, reporter genes have just entered their fourth decade. Initial studies relied on the simple fusion of a promoter or gene with a particular reporter gene of interest. Such constructs were then used to determine the promoter activity unde...
|
PMID: 20663405
PDF is available here.
PDF is available here.
Human Mutation (31)7 2010
Abstract
We identified a missense mutation (c.499T>C; p.Cys167Arg) in exon 2 of the SOST gene. This type of mutation has not been previously reported and using different functional approaches, we show that it has a devastating effect on the biological function of sclerostin. The affected cysteine is the last...
|
PMID: 20583295
PDF is available here.
PDF is available here.
Human Mutation (31)7 2010
Abstract
We identified a missense mutation (c.499T>C; p.Cys167Arg) in exon 2 of the SOST gene. This type of mutation has not been previously reported and using different functional approaches, we show that it has a devastating effect on the biological function of sclerostin. The affected cysteine is the last...
|
PMID: 20583295
PDF is available here.
PDF is available here.
Human Mutation (31)7 2010
Abstract
We identified a missense mutation (c.499T>C; p.Cys167Arg) in exon 2 of the SOST gene. This type of mutation has not been previously reported and using different functional approaches, we show that it has a devastating effect on the biological function of sclerostin. The affected cysteine is the last...
|
PMID: 20583295
PDF is available here.
PDF is available here.
Human Mutation (31)7 2010
Abstract
We identified a missense mutation (c.499T>C; p.Cys167Arg) in exon 2 of the SOST gene. This type of mutation has not been previously reported and using different functional approaches, we show that it has a devastating effect on the biological function of sclerostin. The affected cysteine is the last...
|
PMID: 20583295
PDF is available here.
PDF is available here.
Human Mutation (31)7 2010
Abstract
We identified a missense mutation (c.499T>C; p.Cys167Arg) in exon 2 of the SOST gene. This type of mutation has not been previously reported and using different functional approaches, we show that it has a devastating effect on the biological function of sclerostin. The affected cysteine is the last...
|
PMID: 20583295
PDF is available here.
PDF is available here.
Human Mutation (31)7 2010
Abstract
We identified a missense mutation (c.499T>C; p.Cys167Arg) in exon 2 of the SOST gene. This type of mutation has not been previously reported and using different functional approaches, we show that it has a devastating effect on the biological function of sclerostin. The affected cysteine is the last...
|
PMID: 20583295
PDF is available here.
PDF is available here.
Human Mutation (31)7 2010
Abstract
We identified a missense mutation (c.499T>C; p.Cys167Arg) in exon 2 of the SOST gene. This type of mutation has not been previously reported and using different functional approaches, we show that it has a devastating effect on the biological function of sclerostin. The affected cysteine is the last...
|
PMID: 20583295
PDF is available here.
PDF is available here.
Human Mutation (31)7 2010
Abstract
We identified a missense mutation (c.499T>C; p.Cys167Arg) in exon 2 of the SOST gene. This type of mutation has not been previously reported and using different functional approaches, we show that it has a devastating effect on the biological function of sclerostin. The affected cysteine is the last...
|
PMID: 20583295
PDF is available here.
PDF is available here.
Human Mutation (31)7 2010
Abstract
We identified a missense mutation (c.499T>C; p.Cys167Arg) in exon 2 of the SOST gene. This type of mutation has not been previously reported and using different functional approaches, we show that it has a devastating effect on the biological function of sclerostin. The affected cysteine is the last...
|
PMID: 20583295
PDF is available here.
PDF is available here.
Human Mutation (31)7 2010
Abstract
We identified a missense mutation (c.499T>C; p.Cys167Arg) in exon 2 of the SOST gene. This type of mutation has not been previously reported and using different functional approaches, we show that it has a devastating effect on the biological function of sclerostin. The affected cysteine is the last...
|
PMID: 20583295
PDF is available here.
PDF is available here.
Human Mutation (31)7 2010
Abstract
We identified a missense mutation (c.499T>C; p.Cys167Arg) in exon 2 of the SOST gene. This type of mutation has not been previously reported and using different functional approaches, we show that it has a devastating effect on the biological function of sclerostin. The affected cysteine is the last...
|
PMID: 20583295
PDF is available here.
PDF is available here.
Human Mutation (31)7 2010
Abstract
We identified a missense mutation (c.499T>C; p.Cys167Arg) in exon 2 of the SOST gene. This type of mutation has not been previously reported and using different functional approaches, we show that it has a devastating effect on the biological function of sclerostin. The affected cysteine is the last...
|
PMID: 20583295
PDF is available here.
PDF is available here.
Human Mutation (31)7 2010
Abstract
We identified a missense mutation (c.499T>C; p.Cys167Arg) in exon 2 of the SOST gene. This type of mutation has not been previously reported and using different functional approaches, we show that it has a devastating effect on the biological function of sclerostin. The affected cysteine is the last...
|
PMID: 20583295
PDF is available here.
PDF is available here.
Human Mutation (31)7 2010
Abstract
We identified a missense mutation (c.499T>C; p.Cys167Arg) in exon 2 of the SOST gene. This type of mutation has not been previously reported and using different functional approaches, we show that it has a devastating effect on the biological function of sclerostin. The affected cysteine is the last...
|
PMID: 20583295
PDF is available here.
PDF is available here.
Human Mutation (31)7 2010
Abstract
We identified a missense mutation (c.499T>C; p.Cys167Arg) in exon 2 of the SOST gene. This type of mutation has not been previously reported and using different functional approaches, we show that it has a devastating effect on the biological function of sclerostin. The affected cysteine is the last...
|
PMID: 20583295
PDF is available here.
PDF is available here.
Human Mutation (31)7 2010
Abstract
We identified a missense mutation (c.499T>C; p.Cys167Arg) in exon 2 of the SOST gene. This type of mutation has not been previously reported and using different functional approaches, we show that it has a devastating effect on the biological function of sclerostin. The affected cysteine is the last...
|
PMID: 20583295
PDF is available here.
PDF is available here.
Human Mutation (31)7 2010
Abstract
We identified a missense mutation (c.499T>C; p.Cys167Arg) in exon 2 of the SOST gene. This type of mutation has not been previously reported and using different functional approaches, we show that it has a devastating effect on the biological function of sclerostin. The affected cysteine is the last...
|
PMID: 20583295
PDF is available here.
PDF is available here.
Human Mutation (31)7 2010
Abstract
We identified a missense mutation (c.499T>C; p.Cys167Arg) in exon 2 of the SOST gene. This type of mutation has not been previously reported and using different functional approaches, we show that it has a devastating effect on the biological function of sclerostin. The affected cysteine is the last...
|
PMID: 20583295
PDF is available here.
PDF is available here.
Human Mutation (31)7 2010
Abstract
We identified a missense mutation (c.499T>C; p.Cys167Arg) in exon 2 of the SOST gene. This type of mutation has not been previously reported and using different functional approaches, we show that it has a devastating effect on the biological function of sclerostin. The affected cysteine is the last...
|
PMID: 20583295
PDF is available here.
PDF is available here.
Human Mutation (31)7 2010
Abstract
We identified a missense mutation (c.499T>C; p.Cys167Arg) in exon 2 of the SOST gene. This type of mutation has not been previously reported and using different functional approaches, we show that it has a devastating effect on the biological function of sclerostin. The affected cysteine is the last...
|
PMID: 20583295
PDF is available here.
PDF is available here.
Human Mutation (31)7 2010
Abstract
We identified a missense mutation (c.499T>C; p.Cys167Arg) in exon 2 of the SOST gene. This type of mutation has not been previously reported and using different functional approaches, we show that it has a devastating effect on the biological function of sclerostin. The affected cysteine is the last...
|
PMID: 20583295
PDF is available here.
PDF is available here.
Human Mutation (31)7 2010
Abstract
We identified a missense mutation (c.499T>C; p.Cys167Arg) in exon 2 of the SOST gene. This type of mutation has not been previously reported and using different functional approaches, we show that it has a devastating effect on the biological function of sclerostin. The affected cysteine is the last...
|
PMID: 20583295
PDF is available here.
PDF is available here.
Human Mutation (31)7 2010
Abstract
We identified a missense mutation (c.499T>C; p.Cys167Arg) in exon 2 of the SOST gene. This type of mutation has not been previously reported and using different functional approaches, we show that it has a devastating effect on the biological function of sclerostin. The affected cysteine is the last...
|
PMID: 20583295
PDF is available here.
PDF is available here.
Human Mutation (31)7 2010
Abstract
We identified a missense mutation (c.499T>C; p.Cys167Arg) in exon 2 of the SOST gene. This type of mutation has not been previously reported and using different functional approaches, we show that it has a devastating effect on the biological function of sclerostin. The affected cysteine is the last...
|
PMID: 20583295
PDF is available here.
PDF is available here.
Human Mutation (31)7 2010
Abstract
We identified a missense mutation (c.499T>C; p.Cys167Arg) in exon 2 of the SOST gene. This type of mutation has not been previously reported and using different functional approaches, we show that it has a devastating effect on the biological function of sclerostin. The affected cysteine is the last...
|
PMID: 20583295
PDF is available here.
PDF is available here.
Human Mutation (31)7 2010
Abstract
We identified a missense mutation (c.499T>C; p.Cys167Arg) in exon 2 of the SOST gene. This type of mutation has not been previously reported and using different functional approaches, we show that it has a devastating effect on the biological function of sclerostin. The affected cysteine is the last...
|
PMID: 20583295
PDF is available here.
PDF is available here.
Human Mutation (31)7 2010
Abstract
We identified a missense mutation (c.499T>C; p.Cys167Arg) in exon 2 of the SOST gene. This type of mutation has not been previously reported and using different functional approaches, we show that it has a devastating effect on the biological function of sclerostin. The affected cysteine is the last...
|
PMID: 20583295
PDF is available here.
PDF is available here.
Human Mutation (31)7 2010
Abstract
We identified a missense mutation (c.499T>C; p.Cys167Arg) in exon 2 of the SOST gene. This type of mutation has not been previously reported and using different functional approaches, we show that it has a devastating effect on the biological function of sclerostin. The affected cysteine is the last...
|
PMID: 20583295
PDF is available here.
PDF is available here.
Human Mutation (31)7 2010
Abstract
We identified a missense mutation (c.499T>C; p.Cys167Arg) in exon 2 of the SOST gene. This type of mutation has not been previously reported and using different functional approaches, we show that it has a devastating effect on the biological function of sclerostin. The affected cysteine is the last...
|
PMID: 20583295
PDF is available here.
PDF is available here.
Human Mutation (31)7 2010
Abstract
We identified a missense mutation (c.499T>C; p.Cys167Arg) in exon 2 of the SOST gene. This type of mutation has not been previously reported and using different functional approaches, we show that it has a devastating effect on the biological function of sclerostin. The affected cysteine is the last...
|
PMID: 20583295
PDF is available here.
PDF is available here.
Human Mutation (31)7 2010
Abstract
We identified a missense mutation (c.499T>C; p.Cys167Arg) in exon 2 of the SOST gene. This type of mutation has not been previously reported and using different functional approaches, we show that it has a devastating effect on the biological function of sclerostin. The affected cysteine is the last...
|
PMID: 20583295
PDF is available here.
PDF is available here.
Human Mutation (31)7 2010
Abstract
We identified a missense mutation (c.499T>C; p.Cys167Arg) in exon 2 of the SOST gene. This type of mutation has not been previously reported and using different functional approaches, we show that it has a devastating effect on the biological function of sclerostin. The affected cysteine is the last...
|
PMID: 20583295
PDF is available here.
PDF is available here.
Human Mutation (31)7 2010
Abstract
We identified a missense mutation (c.499T>C; p.Cys167Arg) in exon 2 of the SOST gene. This type of mutation has not been previously reported and using different functional approaches, we show that it has a devastating effect on the biological function of sclerostin. The affected cysteine is the last...
|
PMID: 20583295
PDF is available here.
PDF is available here.
Human Mutation (31)7 2010
Abstract
We identified a missense mutation (c.499T>C; p.Cys167Arg) in exon 2 of the SOST gene. This type of mutation has not been previously reported and using different functional approaches, we show that it has a devastating effect on the biological function of sclerostin. The affected cysteine is the last...
|
PMID: 20583295
PDF is available here.
PDF is available here.
Human Mutation (31)7 2010
Abstract
We identified a missense mutation (c.499T>C; p.Cys167Arg) in exon 2 of the SOST gene. This type of mutation has not been previously reported and using different functional approaches, we show that it has a devastating effect on the biological function of sclerostin. The affected cysteine is the last...
|
PMID: 20583295
PDF is available here.
PDF is available here.
Human Mutation (31)7 2010
Abstract
We identified a missense mutation (c.499T>C; p.Cys167Arg) in exon 2 of the SOST gene. This type of mutation has not been previously reported and using different functional approaches, we show that it has a devastating effect on the biological function of sclerostin. The affected cysteine is the last...
|
PMID: 20583295
PDF is available here.
PDF is available here.
Gene Therapy (17)7 2010
Abstract
We present data from two independent quantitative studies. Using in vivo bioimaging of a far-red fluorescent molecule, Katushka, allowing for continuous monitoring of local gene expression, compared with measurements of a systemic transgene, that is, serum erythropoietin (EPO) after gene electrotran...
|
PMID: 20376097
PDF is available here.
PDF is available here.
Journal of Bacteriology (192)13 2010
Abstract
DcuS is the membrane-integral sensor histidine kinase of the DcuSR two-component system in Escherichia coli that responds to extracellular C(4)-dicarboxylates. The oligomeric state of full-length DcuS was investigated in vitro and in living cells by chemical cross-linking and by fluorescence resonan...
|
PMID: 20453099
PDF is available here.
PDF is available here.
Abstract
Abstract
We show here that specific stimulation of local CaMKIIalpha-expressing excitatory neurons, either in the neocortex or thalamus, elicits positive BOLD signals at the stimulus location with classical kinetics. We also show that optogenetic fMRI (of MRI) allows visualization of the causal effects of sp...
|
PMID: 20473285
PDF is available here.
PDF is available here.
Abstract
Abstract
We sought to directly identify IL-1beta-producing cells in living animals by construction of transgenic mice expressing DsRed fluorescence protein gene under the control of IL-1beta promoter. Little DsRed fluorescence signal was detected in skin under steady-state conditions. Striking increases in D...
|
PMID: 20147964
PDF is available here.
PDF is available here.