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Calnexin (0):
Recent article
Calnexin
Abstract
We show that Malectin is an ER stress-induced protein that selectively associates with glycopolypeptides without affecting their entry and their retention in the Calnexin chaperone system. Analysis of the obligate Calnexin client influenza virus hemagglutinin (HA) revealed that Calnexin and Malectin...
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PMID: 21298103
PDF is available here.
PDF is available here.
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Abstract
We first analyzed donor specificity of UGGT, which was proven to be rather narrow. However, marginal activity was observed with UDP-galactose and UDP-glucuronic acid as well as with 3-, 4- and 6-deoxy glucose analogues to give corresponding transfer products. Intriguingly, G-II smoothly converted al...
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PMID: 21075077
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (285)43 2010
Abstract
We examined the interaction of kAE1 with the quality control system responsible for the folding of membrane glycoproteins and the retention and degradation of misfolded mutants. Using small molecule inhibitors to disrupt chaperone interactions, two functional, dominant kAE1 mutants (R589H and R901st...
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PMID: 20628050
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (285)43 2010
Abstract
We report that the transmembrane segments of Na(v)1.8 also cause this channel retained in the ER. Using transferrin receptor and CD8α as model molecules, immunocytochemistry showed that the first, second, and third transmembrane segments in each domain of Na(v)1.8 reduced their surface expression....
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PMID: 20720009
PDF is available here.
PDF is available here.
Biophysical Journal (99)4 2010
Abstract
We have used fluorescence correlation spectroscopy to probe these interactions for a prototypical transmembrane protein, VSVG ts045, in vivo. While both folded and unfolded VSVG ts045 showed anomalous diffusion, the unfolded protein had a significantly stronger anomaly. This difference subsided when...
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PMID: 20713018
PDF is available here.
PDF is available here.
Abstract
Human 29IJ6 IgG was expressed in silkworm using a Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid system. The mean amounts of 296IJ6 IgG produced in larval hemolymph and whole pupae were 30.1 microg/larva and 78.0 microg/pupa, respectively. The use of molecular chaperones including calreticulin (CRT...
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PMID: 19918885
PDF is available here.
PDF is available here.
Biology of Reproduction (81)2 2009
Abstract
We have used fluorescence-activated cell sorting to purify germ cells from mouse fetal testes and ovaries and examined 16 common housekeeping genes for their suitability as reference genes. In pure populations of germ cells isolated from Embryonic Day 12.5 (E12.5) to E15.5 male and female gonads, Ma...
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PMID: 19403927
PDF is available here.
PDF is available here.
Abstract
Recurrence and persistent side effects of present day treatment for urolithiasis restrict their use, so an alternate, using phytotherapy is being sought. Dolichos biflorus seeds, which are used as dietary food in India, possess antilithiatic properties. In the present study, a novel dimeric antilith...
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PMID: 19488841
PDF is available here.
PDF is available here.
Abstract
We show that yeast (Saccharomyces cerevisiae) can expose the CRT orthologue CNE1 on the surface in response to cell death induced by the anthracylin mitoxantrone (MTX). This MTX-triggered CNE1 translocation is abolished by knockout of the yeast orthologues of PERK (Gcn2), BAP31 (Yet3) and SNAREs (Ny...
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PMID: 19182525
PDF is available here.
PDF is available here.
Abstract
We investigated the mechanism underlying ERp57 specificity toward glycoprotein substrates and the interdependence of ERp57 and the calnexin cycle for their correct folding. Our results clearly show that ERp57 must be physically associated with the calnexin cycle to catalyze isomerization reactions w...
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PMID: 19054761
PDF is available here.
PDF is available here.
Terapevticheskii arkhiv (81)7 2009
Abstract
Lowering of cultivation temperature did not increase ACE expression. Intracellular shaperon Bip rose insignificantly (1.3-fold) in DC of the controls. ACE and Bip shaperon expression on LDC membrane increased 15- and 11-fold, respectively, while the level of intracellular ACE and Bip decreased 11- a...
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PMID: 19708569
PDF is available here.
PDF is available here.
Cellular Microbiology (10)12 2008
Abstract
We identify the unique 20 kDa PtdIns(4)P-binding domain of SidC, which upon heterologous expression in Dictyostelium binds to LCVs and thus is useful as a PtdIns(4)P-specific probe. LCVs harbouring L. pneumophilaDeltasidC-sdcA mutant bacteria recruit ER and ER-derived vesicles less efficiently and c...
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PMID: 18673369
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (283)49 2008
Abstract
The earliest steps in nascent protein maturation greatly affect its overall efficiency. Constraints placed on maturing proteins at these early stages limit available conformations and help to direct the native maturation process. For type II membrane proteins, these cotranslational constraints inclu...
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PMID: 18849342
PDF is available here.
PDF is available here.
Abstract
We examined levels of mRNA and protein expression of MHC class I proteins, as well as several MHC class I antigen processing and presentation chaperones in mouse ES cells derived from both fertilized (fES) and parthenogenetic (pES) embryos. We found that H-2K, Qa-2, TAP1, TAP2, and tapasin mRNAs wer...
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PMID: 18778324
PDF is available here.
PDF is available here.
Abstract
We identified a dependence on calnexin for proper assembly of CFTR's membrane spanning domains. Interestingly, efficient folding of NBD2 was also found to be dependent upon calnexin binding to CFTR. Furthermore, we identified folding defects caused by deletion of F508 that occurred before and after...
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PMID: 18716059
PDF is available here.
PDF is available here.
Abstract
We report that overexpression of calnexin in Schizosaccharomyces pombe induces cell death with apoptosis markers. Cell death was partially dependent on the Ire1p ER-stress transducer. Apoptotic death caused by calnexin overexpression required its transmembrane domain (TM), and involved sequences on...
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PMID: 18701708
PDF is available here.
PDF is available here.
Abstract
We have studied the possible differences among L1210 and L1210/VCR cells in expression of endoplasmic reticulum proteins involved in the regulation of calcium homeostasis and calcium-dependent processes. Amounts of mRNA encoding both calcium release channels (ryanodine receptor channels--RyR and IP3...
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PMID: 18981537
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1783)9 2008
Abstract
We show that calnexin (CNX) is not necessary for the ER retention of DeltaF508 CFTR. Our data show that CNX knockout (KO) does not affect the biosynthetic processing, cellular localization or the Cl(-) channel function of DeltaF508 CFTR. Importantly, cAMP-induced Cl(-) current in colonic epithelium...
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PMID: 18457676
PDF is available here.
PDF is available here.
Journal of Cell Science (121)Pt 17 2008
Abstract
We investigated the role of N-linked glycosylation in the processing of wild-type and DeltaF508 CFTR. We found that N-glycosylation and ER lectin interactions are not major determinants of trafficking of wild-type and DeltaF508 from the ER to the plasma membrane. Unglycosylated CFTR, generated by re...
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PMID: 18682497
PDF is available here.
PDF is available here.
Abstract
We detected a 1.5 Kb transcript of human heparanase by RT-PCR. The smaller transcript was shown to be an alternatively spliced variant lacking exon 5, which contains the essential glutamic acid residue required for enzyme activity. When expressed in COS-7 cells this variant did not show any heparana...
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PMID: 18646256
PDF is available here.
PDF is available here.
Journal of Neurochemistry (106)2 2008
Abstract
We examined expression and function of 14-3-3 isoforms in vitro using mouse organotypic hippocampal cultures. Treatment of cultures with the endoplasmic reticulum (ER) stressor tunicamycin caused an increase in levels of 14-3-3 zeta within the ER-containing microsomal fraction, along with up-regulat...
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PMID: 18466333
PDF is available here.
PDF is available here.
Abstract
We have investigated how overexpression of TRPC3 interferes with the regulation of intracellular calcium stores. We demonstrate that overexpression of TRPC3 reduces the mobilization of calcium in response to stimulation of the cells with thapsigargin (TG) or the G-protein coupled receptor agonist sp...
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PMID: 18431718
PDF is available here.
PDF is available here.
Journal of Virology (82)11 2008
Abstract
We evaluated by RNA interference the relevance to rotavirus assembly and infectivity of grp78, protein disulfide isomerase (PDI), grp94, calnexin, calreticulin, and ERp57, members of the two ER folding systems described herein. Silencing the expression of grp94 and Erp57 had no effect on rotavirus i...
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PMID: 18385250
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1783)6 2008
Abstract
We show a significant increase in the expression of genes involved in ER associated degradation and activation of the ubiquitin-proteasome system in crt-/- cells. We also demonstrated that the ubiquitination of two proteins processed in ER, connexin 43 and A1AT NHK (alpha1-antitrypsin mutant) are in...
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PMID: 18405668
PDF is available here.
PDF is available here.
Journal of General Virology (89)Pt 5 2008
Abstract
We utilized a chimeric class I heavy chain with an affinity tag at its carboxy terminus. Interestingly, US2 but not US11 continued to target the class I chimera for destruction, suggesting a structural limitation for US11-mediated degradation. Association studies in US2 cells and in cells that expre...
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PMID: 18420789
PDF is available here.
PDF is available here.
Journal of Molecular Biology (378)2 2008
Abstract
We previously identified mutations, e.g., in the gamma-aminobutyric acid (GABA) transporter-1 (GAT1), which disrupted assembly and caused retention of the transporter in the ER. Using one representative mutant, GAT1-E101D, we showed here that ER retention was due to association of the transporter wi...
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PMID: 18367207
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (283)16 2008
Abstract
Abstract
We show that, analogously to the pTalpha chain, surface CNX is palmitoylated and that CD3 prominently accumulated in lipid rafts upon cross-linking. Mutant CNX isoforms devoid of ER retention determined pre-TCR-like signaling and simulated beta selection only when stably translocating CD3 to lipid r...
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PMID: 18350545
PDF is available here.
PDF is available here.
Human Molecular Genetics (17)4 2008
Abstract
We previously reported that neuronal death by apoptosis in INCL, and in the PPT1-knockout (PPT1-KO) mice that mimic INCL, is at least in part caused by endoplasmic reticulum (ER) and oxidative stresses. In the present study, we sought to determine whether ER and oxidative stresses are unique manifes...
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PMID: 17989065
PDF is available here.
PDF is available here.
Abstract
We found that dendritic cells (DCs) from multiple myeloma (MM) patients suppressed rather than induced a cancer cell-specific immune response. We demonstrated that CD4(+)CD25(high) T cells from MM patients suppressed the proliferation of activated peripheral blood lymphocytes. Further analysis illus...
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PMID: 18071334
PDF is available here.
PDF is available here.
Molecular Endocrinology (22)2 2008
Abstract
We have examined the intracellular fate of recombinant mutant Tg expressed in COS-7 cells. Incorporation of the R19K mutation largely blocked Tg secretion, and this mutant was approximately 90% degraded intracellularly over a 24-h period after synthesis. Before its degradation, the Tg-R19K mutant ex...
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PMID: 17916655
PDF is available here.
PDF is available here.
Abstract
We found that in hepatoma cells, mutated alpha1-antitrypsin exited the ER and accumulated in IBs that were negative for autophagosomal and lysosomal markers, and contained several ER components, but not calnexin. Mutated alpha1-antitrypsin induced IBs also in neuroendocrine cells, showing that forma...
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PMID: 18045994
PDF is available here.
PDF is available here.
Annals of Hematology (87)2 2008
Abstract
Recently, it was shown that glycoproteins with N-glycans close to the NH(2) terminus can directly enter the calnexin/calreticulin cycle and bypass BiP binding. This should allow efficient secretion of glycoproteins such as factor VIII (FVIII) whose secretion is negatively affected by BiP interaction...
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PMID: 17899080
PDF is available here.
PDF is available here.
Abstract
We cultured calnexin-disrupted and wild-type Saccharomyces cerevisiae strains under conditions of heat stress. The growth rate of the calnexin-disrupted yeast was almost the same as that of the wild-type yeast under those conditions. However, the induced mRNA level of the molecular chaperone PDI in...
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PMID: 17952377
PDF is available here.
PDF is available here.
Abstract
A unique feature of the intracellular life cycle of Legionella pneumophila is the interaction between the vacuole in which L. pneumophila resides and the endoplasmic reticulum of the host cell. This interaction is crucial for L. pneumophila to establish a niche in which the bacteria can replicate in...
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PMID: 18425463
PDF is available here.
PDF is available here.
Molecular Vision (14) 2008
Abstract
Our data show that, unlike Drosophila Rh1, mammalian rod opsin biogenesis does not appear to have an absolute requirement for Cnx. Other chaperones are likely to be more important for mammalian rod opsin biogenesis and quality control....
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PMID: 19116670
PDF is available here.
PDF is available here.
Molecular Cell (28)4 2007
Abstract
We used a nonsecreted kappa LC, which exists in partially (ox1) and completely (ox2) oxidized states. The ox2 form is partially reduced in order to be degraded, and only the ox1 form is ubiquitinated and associates with both Herp and Derlin-1. Herp is in a complex with ubiquitinated proteins and wit...
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PMID: 18042451
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (282)47 2007
Abstract
We hypothesized that regulation of the multisubunit machinery assembly is a mechanism by which ERAD activity is regulated. To test this hypothesis, we investigated the potential regulatory role of the small p97/VCP-interacting protein (SVIP) on the formation of the ERAD machinery that includes ubiqu...
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PMID: 17872946
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (282)47 2007
Abstract
We have previously shown that high-grade glioma cells functionally express this current because of the lack of ASIC2 in the plasma membrane. We now hypothesize that ASIC2 trafficking in glioma cells is regulated by a specific chaperone protein, namely Hsc70. Our results demonstrated that Hsc70 co-im...
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PMID: 17878160
PDF is available here.
PDF is available here.
Acta Neuropathologica (114)5 2007
Abstract
We examined the activation of ER stress-related proteins by immunohistochemistry, western blot analysis and RT-PCR. In four of five DM1 muscle biopsies, except for a muscle biopsy from a patient with the shortest CTG expansion and no myotonia, increased expression of GRP78 and calnexin, and phosphor...
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PMID: 17661063
PDF is available here.
PDF is available here.
Abstract
We have found evidence for ER stress occurring during development of the central nervous system in the mouse. Several ER-resident stress-regulated chaperones, such as calreticulin, glucose regulated protein 78, glucose regulated protein 94, ER protein 57 and protein disulfide isomerase, were express...
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PMID: 17913437
PDF is available here.
PDF is available here.
Abstract
We developed affinity chromatography by conjugating synthetic N-glycans on sepharose beads. Using the affinity beads with the dodecasaccharide Glc(1)Man(9)GlcNAc(2), one structure of the N-glycans, a 75-kDa protein, was isolated from the membranous fraction including the ER in Aspergillus oryzae. By...
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PMID: 17986781
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (282)43 2007
Abstract
Our results suggest that interactions with the chaperones regulate the levels of nAChRs assembled in the ER by stabilizing and sequestering subunits during assembly....
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PMID: 17728248
PDF is available here.
PDF is available here.
Biology of the Cell (99)10 2007
Abstract
As expression of truncated LBR-GFP in plant cells results in altered targeting and retention compared with wtLBR-GFP, we conclude that plant cells can recognize the INE (inner NE)-targeting motif of LBR. The altered mobility of the truncated protein suggests that not only do plant cells recognize th...
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PMID: 17868028
PDF is available here.
PDF is available here.
Abstract
These results indicate that the carbohydrate chain attached to Asn-292 of OVA has an important role for the secretion and folding in the cells....
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PMID: 17678626
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (282)38 2007
Abstract
We examined the biosynthesis, function, and spectral properties of the peroxidase stably expressed in human embryonic kidney cells. Biosynthesis of normal MPO by human embryonic kidney cells faithfully mirrored events previously identified in cells expressing endogenous MPO. Mutant apopro-MPO was 90...
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PMID: 17650507
PDF is available here.
PDF is available here.
Abstract
We have identified HMGB1 as a CpG-ODN-binding protein. HMGB1 interacts and preassociates with TLR9 in the endoplasmic reticulum-Golgi intermediate compartment (ERGIC), and hastens TLR9's redistribution to early endosomes in response to CpG-ODN. CpG-ODN stimulates macrophages and dendritic cells to s...
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PMID: 17548579
PDF is available here.
PDF is available here.
Abstract
We first replicated our previous results with a modified protocol that generated aggregated alpha-synuclein more efficiently. Next, using two recently developed proteomic techniques, SILAC (Stable Isotope Labeling of Amino acid in Cell cultures) and PROCEED (PROteome of Cell Exposed Extracellular Do...
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PMID: 17676786
PDF is available here.
PDF is available here.
Journal of Molecular Biology (371)3 2007
Abstract
We demonstrate that the sarco(endo)plasmic reticulum Ca(2+) ATPase 2b (SERCA2b), which maintains high Ca(2+) concentration in the lumen of the endoplasmic reticulum, interacts specifically with the human delta opioid receptor during early steps of receptor biogenesis in human embryonic kidney 293 ce...
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PMID: 17588601
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (282)32 2007
Abstract
We directly tested this hypothesis using wild-type and mutant forms of the human delta-opioid receptor as models. ER-localized receptors were isolated by expressing the receptors in HEK293 cells under tightly controlled tetracycline induction and blocking their ER export with brefeldin A. The ER-ret...
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PMID: 17550902
PDF is available here.
PDF is available here.
Molecular Cell (27)2 2007
Abstract
We show that for certain newly synthesized glycoproteins UGT1 deletion has no effect on binding to calnexin. These proteins must normally complete their folding program in one binding event. Other proteins normally undergo multiple binding events, and UGT1 deletion results in their premature release...
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PMID: 17643373
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (282)29 2007
Abstract
We have now identified the ER chaperone protein calnexin as an interacting protein for both D(1) and D(2) dopamine receptors. These protein-protein interactions were confirmed using Western blot analysis and co-immunoprecipitation experiments. To determine the influence of calnexin on receptor expre...
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PMID: 17395585
PDF is available here.
PDF is available here.
Abstract
We investigated the determinants of Na(v)1.8 expression in tsA201 cells, a transformed variant of HEK293 cells, using a combination of biochemistry, immunochemistry, and electrophysiology. Our data indicate that the unusually low expression levels of Na(v)1.8 in tsA201 cells results from a trafficki...
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PMID: 17507497
PDF is available here.
PDF is available here.
Abstract
Biochemical Journal (404)3 2007
Abstract
Over one-third of all newly synthesized polypeptides in eukaryotes interact with or insert into the membrane or the lumenal space of the ER (endoplasmic reticulum), an event that is essential for the subsequent folding, post-translational modification, assembly and targeting of these proteins. Conse...
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PMID: 17521290
PDF is available here.
PDF is available here.
Cardiovascular Research (74)1 2007
Abstract
Phospholamban and sarcolipin are maintained in the SR/ER by a process that includes their retrieval from the ERGIC following their passage from the SR/ER into the ERGIC. The transmembrane domains of phospholamban and sarcolipin are involved in the retrieval process....
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PMID: 17307155
PDF is available here.
PDF is available here.
Abstract
We examined the biogenesis of Legionella-containing vacuoles (LCVs) formed in permissive A/J macrophages and in their Birc1e/Naip5 transgenic non-permissive counterpart. Birc1e/Naip5 effects on acquisition of lysosomal and ER markers were evident within 1-2 h following infection. A significantly hig...
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PMID: 17087731
PDF is available here.
PDF is available here.
Journal of Neurotrauma (24)4 2007
Abstract
We investigated the effects of brain trauma on the expression of a number of stress genes by in situ hybridization and Western blot analysis including the endoplasmic reticulum (ER) stress gene grp78, ER protein processing enzymes calnexin and protein disulphide isomerase (PDI), the mitochondrial st...
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PMID: 17439344
PDF is available here.
PDF is available here.
FEMS Yeast Research (7)2 2007
Abstract
We recently reported that, under certain circumstances, S. pombe cells are able to survive in the absence of calnexin/Cnx1p, indicating that an inducible pathway can complement the calnexin/Cnx1p essential function(s). This calnexin-independent state (Cin) is transmitted by a nonchromosomal proteina...
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PMID: 17328741
PDF is available here.
PDF is available here.
Abstract
We demonstrate that MCF-7 cells are resistant to tunicamycin-induced apoptosis. We show that the expression level of the ER chaperone calnexin can directly influence tunicamycin sensitivity in this cell line. Interestingly, the expression of a calnexin lacking the chaperone domain (DeltaE) partially...
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PMID: 16858427
PDF is available here.
PDF is available here.