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Pore Forming Cytotoxic Proteins (7):
Recent article
Pore Forming Cytotoxic Proteins
Infection and Immunity (78)8 2010
Abstract
We developed an infection model in murine neonates using the gastroenteric pathogen Yersinia enterocolitica. Here, we report that oral infection of neonatal mice with low doses of virulent Y. enterocolitica leads to vigorous intestinal and systemic adaptive immunity. Y. enterocolitica infection prom...
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PMID: 20515925
PDF is available here.
PDF is available here.
| < More recent | Older article > |
Expression of an immunogenic F1-V fusion protein in lettuce as a plant-based vaccine against plague.
Abstract
Yersinia pestis is a pathogenic agent that causes the bubonic and pneumonic plague. The development of an efficient and low-cost oral vaccine against these diseases is highly desirable. In this study, the immunogenic fusion protein F1-V from Y. pestis was introduced into lettuce via...
|
PMID: 20461403
PDF is available here.
PDF is available here.
Expression of an immunogenic F1-V fusion protein in lettuce as a plant-based vaccine against plague.
Abstract
Yersinia pestis is a pathogenic agent that causes the bubonic and pneumonic plague. The development of an efficient and low-cost oral vaccine against these diseases is highly desirable. In this study, the immunogenic fusion protein F1-V from Y. pestis was introduced into lettuce via...
|
PMID: 20461403
PDF is available here.
PDF is available here.
Expression of an immunogenic F1-V fusion protein in lettuce as a plant-based vaccine against plague.
Abstract
Yersinia pestis is a pathogenic agent that causes the bubonic and pneumonic plague. The development of an efficient and low-cost oral vaccine against these diseases is highly desirable. In this study, the immunogenic fusion protein F1-V from Y. pestis was introduced into lettuce via...
|
PMID: 20461403
PDF is available here.
PDF is available here.
Expression of an immunogenic F1-V fusion protein in lettuce as a plant-based vaccine against plague.
Abstract
Yersinia pestis is a pathogenic agent that causes the bubonic and pneumonic plague. The development of an efficient and low-cost oral vaccine against these diseases is highly desirable. In this study, the immunogenic fusion protein F1-V from Y. pestis was introduced into lettuce via...
|
PMID: 20461403
PDF is available here.
PDF is available here.
Expression of an immunogenic F1-V fusion protein in lettuce as a plant-based vaccine against plague.
Abstract
Yersinia pestis is a pathogenic agent that causes the bubonic and pneumonic plague. The development of an efficient and low-cost oral vaccine against these diseases is highly desirable. In this study, the immunogenic fusion protein F1-V from Y. pestis was introduced into lettuce via...
|
PMID: 20461403
PDF is available here.
PDF is available here.
Expression of an immunogenic F1-V fusion protein in lettuce as a plant-based vaccine against plague.
Abstract
Yersinia pestis is a pathogenic agent that causes the bubonic and pneumonic plague. The development of an efficient and low-cost oral vaccine against these diseases is highly desirable. In this study, the immunogenic fusion protein F1-V from Y. pestis was introduced into lettuce via...
|
PMID: 20461403
PDF is available here.
PDF is available here.
Expression of an immunogenic F1-V fusion protein in lettuce as a plant-based vaccine against plague.
Abstract
Yersinia pestis is a pathogenic agent that causes the bubonic and pneumonic plague. The development of an efficient and low-cost oral vaccine against these diseases is highly desirable. In this study, the immunogenic fusion protein F1-V from Y. pestis was introduced into lettuce via...
|
PMID: 20461403
PDF is available here.
PDF is available here.
Expression of an immunogenic F1-V fusion protein in lettuce as a plant-based vaccine against plague.
Abstract
Yersinia pestis is a pathogenic agent that causes the bubonic and pneumonic plague. The development of an efficient and low-cost oral vaccine against these diseases is highly desirable. In this study, the immunogenic fusion protein F1-V from Y. pestis was introduced into lettuce via...
|
PMID: 20461403
PDF is available here.
PDF is available here.
Expression of an immunogenic F1-V fusion protein in lettuce as a plant-based vaccine against plague.
Abstract
Yersinia pestis is a pathogenic agent that causes the bubonic and pneumonic plague. The development of an efficient and low-cost oral vaccine against these diseases is highly desirable. In this study, the immunogenic fusion protein F1-V from Y. pestis was introduced into lettuce via...
|
PMID: 20461403
PDF is available here.
PDF is available here.
Expression of an immunogenic F1-V fusion protein in lettuce as a plant-based vaccine against plague.
Abstract
Yersinia pestis is a pathogenic agent that causes the bubonic and pneumonic plague. The development of an efficient and low-cost oral vaccine against these diseases is highly desirable. In this study, the immunogenic fusion protein F1-V from Y. pestis was introduced into lettuce via...
|
PMID: 20461403
PDF is available here.
PDF is available here.
Expression of an immunogenic F1-V fusion protein in lettuce as a plant-based vaccine against plague.
Abstract
Yersinia pestis is a pathogenic agent that causes the bubonic and pneumonic plague. The development of an efficient and low-cost oral vaccine against these diseases is highly desirable. In this study, the immunogenic fusion protein F1-V from Y. pestis was introduced into lettuce via...
|
PMID: 20461403
PDF is available here.
PDF is available here.
Expression of an immunogenic F1-V fusion protein in lettuce as a plant-based vaccine against plague.
Abstract
Yersinia pestis is a pathogenic agent that causes the bubonic and pneumonic plague. The development of an efficient and low-cost oral vaccine against these diseases is highly desirable. In this study, the immunogenic fusion protein F1-V from Y. pestis was introduced into lettuce via...
|
PMID: 20461403
PDF is available here.
PDF is available here.
Expression of an immunogenic F1-V fusion protein in lettuce as a plant-based vaccine against plague.
Abstract
Yersinia pestis is a pathogenic agent that causes the bubonic and pneumonic plague. The development of an efficient and low-cost oral vaccine against these diseases is highly desirable. In this study, the immunogenic fusion protein F1-V from Y. pestis was introduced into lettuce via...
|
PMID: 20461403
PDF is available here.
PDF is available here.
Expression of an immunogenic F1-V fusion protein in lettuce as a plant-based vaccine against plague.
Abstract
Yersinia pestis is a pathogenic agent that causes the bubonic and pneumonic plague. The development of an efficient and low-cost oral vaccine against these diseases is highly desirable. In this study, the immunogenic fusion protein F1-V from Y. pestis was introduced into lettuce via...
|
PMID: 20461403
PDF is available here.
PDF is available here.
Expression of an immunogenic F1-V fusion protein in lettuce as a plant-based vaccine against plague.
Abstract
Yersinia pestis is a pathogenic agent that causes the bubonic and pneumonic plague. The development of an efficient and low-cost oral vaccine against these diseases is highly desirable. In this study, the immunogenic fusion protein F1-V from Y. pestis was introduced into lettuce via...
|
PMID: 20461403
PDF is available here.
PDF is available here.
Expression of an immunogenic F1-V fusion protein in lettuce as a plant-based vaccine against plague.
Abstract
Yersinia pestis is a pathogenic agent that causes the bubonic and pneumonic plague. The development of an efficient and low-cost oral vaccine against these diseases is highly desirable. In this study, the immunogenic fusion protein F1-V from Y. pestis was introduced into lettuce via...
|
PMID: 20461403
PDF is available here.
PDF is available here.
Expression of an immunogenic F1-V fusion protein in lettuce as a plant-based vaccine against plague.
Abstract
Yersinia pestis is a pathogenic agent that causes the bubonic and pneumonic plague. The development of an efficient and low-cost oral vaccine against these diseases is highly desirable. In this study, the immunogenic fusion protein F1-V from Y. pestis was introduced into lettuce via...
|
PMID: 20461403
PDF is available here.
PDF is available here.
Expression of an immunogenic F1-V fusion protein in lettuce as a plant-based vaccine against plague.
Abstract
Yersinia pestis is a pathogenic agent that causes the bubonic and pneumonic plague. The development of an efficient and low-cost oral vaccine against these diseases is highly desirable. In this study, the immunogenic fusion protein F1-V from Y. pestis was introduced into lettuce via...
|
PMID: 20461403
PDF is available here.
PDF is available here.
Expression of an immunogenic F1-V fusion protein in lettuce as a plant-based vaccine against plague.
Abstract
Yersinia pestis is a pathogenic agent that causes the bubonic and pneumonic plague. The development of an efficient and low-cost oral vaccine against these diseases is highly desirable. In this study, the immunogenic fusion protein F1-V from Y. pestis was introduced into lettuce via...
|
PMID: 20461403
PDF is available here.
PDF is available here.
Expression of an immunogenic F1-V fusion protein in lettuce as a plant-based vaccine against plague.
Abstract
Yersinia pestis is a pathogenic agent that causes the bubonic and pneumonic plague. The development of an efficient and low-cost oral vaccine against these diseases is highly desirable. In this study, the immunogenic fusion protein F1-V from Y. pestis was introduced into lettuce via...
|
PMID: 20461403
PDF is available here.
PDF is available here.
Gene Therapy (17)7 2010
Abstract
These results indicate that affinity maturation of a neutralizing Ab delivered by genetic transfer may confer increased protection not only for Y. pestis challenge but also possibly for other pathogens....
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PMID: 20393511
PDF is available here.
PDF is available here.
Microbial Pathogenesis (48)6 2010
Abstract
The type III secretion system of Pseudomonas aeruginosa, responsible for acute infection, is composed of over twenty proteins that facilitate cytotoxin injection directly into host cells. Integral to this process is production and secretion of PcrV. Administration of a recently developed, anti-PcrV...
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PMID: 20211240
PDF is available here.
PDF is available here.
Expression of an immunogenic F1-V fusion protein in lettuce as a plant-based vaccine against plague.
Abstract
Yersinia pestis is a pathogenic agent that causes the bubonic and pneumonic plague. The development of an efficient and low-cost oral vaccine against these diseases is highly desirable. In this study, the immunogenic fusion protein F1-V from Y. pestis was introduced into lettuce via...
|
PMID: 20461403
PDF is available here.
PDF is available here.
Zhonghua Yixue Zazhi (90)10 2010
Abstract
In comparison with other groups, the expressions of OPN, IL-15, PFP and GraB mRNA in AR group were gradually up-regulated and peaked at Day 5. The expressions of IL-15 mRNA in CsA and AB groups were 9685 +/- 1440 and 4346 +/- 741 respectively at Day 5 post-operation. It was significantly lower than...
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PMID: 20450731
PDF is available here.
PDF is available here.
Abstract
Long-term protection and antibody response for the subunit vaccine F1-rV270 were determined by using the mouse model. Antibodies to F1 and rV270 were still detectable over a period of 518 days. The complete protection against lethal challenge of Yersinia pestis could be achieved up to day 518 after...
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PMID: 19940042
PDF is available here.
PDF is available here.
Abstract
In every living cell, the lipid bilayer membrane is the ultimate boundary between the contents of the cell and the rest of universe. A single breach in this critical barrier is lethal. For this reason, the bilayer's permeability barrier is the point of attack of many offensive and defensive molecule...
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PMID: 20687477
PDF is available here.
PDF is available here.
Abstract
Colicins are water soluble toxins secreted by E. coli cells to kill other E. coli and related species. To do this they need to cross the outer membrane, periplasm and inner membrane. Pore forming colicins, as their name suggests form a voltage dependent pore in the inner membrane. This chapter deals...
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PMID: 20687482
PDF is available here.
PDF is available here.
Abstract
Bilayer lipids, far from being passive elements, have multiple roles in polypeptide-dependent pore formation. Lipids participate at all stages of the formation of pores by providing the binding site for proteins and peptides, conditioning their active structure and modulating the molecular reorganiz...
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PMID: 20687479
PDF is available here.
PDF is available here.
Abstract
We suggest that similar physical steps could play a role in the mediation of other pore forming toxins (PFT). The membrane pores formed by PFT are expected to have limited lifetime on biological cell surface as a result of membrane dynamics during endocytosis and/or rearrangement of lipid rafts....
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PMID: 20687487
PDF is available here.
PDF is available here.
Abstract
Actinoporins are potent pore-forming toxins produced by sea anemones. They readily form pores in membranes that contain sphingomyelin. Molecular mechanism of pore formation involves recognition of membrane sphingomyelin, firm binding to the membrane accompanied by the transfer of the N-terminal regi...
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PMID: 20687484
PDF is available here.
PDF is available here.
Abstract
Membrane-active peptides exhibit antimicrobial, channel-forming and transport activities and have therefore early on been interesting targets for biophysical investigations. When the peptide-lipid interactions are studied a dynamic view emerges in which the peptides change conformation upon membrane...
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PMID: 20687478
PDF is available here.
PDF is available here.
Abstract
The parasitic mushroom Laetiporus sulphureus produces a family of lectins (LSL's) sharing 80-90% sequence identity that possesses a low but significant sequence similarity to the bacterial pore-forming toxins mosquitocidal toxin Mtx-2 from Bacillus sphaericus and a toxin from Clostridium septicum. T...
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PMID: 20687481
PDF is available here.
PDF is available here.
Abstract
I aim to provide an overview of our understanding of the interaction between CDCs and other members of what is now called the MACPF/CDC superfamily, with their target membranes. CDCs (also in the past known as thiol-activated toxins or cholesterol-binding toxins) were originally identified from four...
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PMID: 20687480
PDF is available here.
PDF is available here.
BMC Cancer (10) 2010
Abstract
PaCa 5061 cells grew as an adhering monolayer with a doubling time ranging from 30 to 48 hours. M-FISH analyses showed a hypertriploid complex karyotype with multiple numerical and unbalanced structural aberrations. Numerous genes were overexpressed, some of which have previously been implicated in...
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PMID: 20553613
PDF is available here.
PDF is available here.
Abstract
The most relevant results obtained suggest that HLA-DR+ Treg cells tend to present higher gene expression of molecules associated with contact-dependent cell activation and cytotoxicity....
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PMID: 20635785
PDF is available here.
PDF is available here.
Abstract
Bacterial toxins damage the host at the site of bacterial infection or distant from the site. Bacterial toxins can be single proteins or oligomeric protein complexes that are organized with distinct AB structure-function properties. The A domain encodes a catalytic activity. ADP ribosylation of host...
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PMID: 20358680
PDF is available here.
PDF is available here.
Chinese Medical Journal (122)23 2009
Abstract
We have identified three patients with three heterozygous missense mutations in PRF1; two of those three mutations (C102F and S108N) have so far been found only from Chinese patients. These findings are useful in evaluating the prevalence of PRF1 mutations in Chinese pediatric patients with HLH, and...
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PMID: 20092789
PDF is available here.
PDF is available here.
Abstract
We undertook a mutational and deletional analysis of the C-terminal helix of apoL1 to investigate the linkage between interaction with SRA and lytic potential for different T. brucei subspecies. We confirm that the C-terminal helix is the SRA-interacting domain. Although in E. coli this domain was d...
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PMID: 19997494
PDF is available here.
PDF is available here.
Abstract
The potential application of Yersinia pestis for bioterrorism emphasizes the urgent need to develop more effective vaccines against airborne infection. The current status of plague vaccines has been reviewed. The present emphasis is on subunit vaccines based on the F1 and LcrV antigens. These provid...
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PMID: 19943765
PDF is available here.
PDF is available here.
Abstract
We show their pharmacological action on guinea-pig and snail models in native and pH-denatured conditions in order to correlate the pharmacological findings with the pore-forming activity of both isoforms. In guinea-pig erythrocytes (N=3), St II possessed higher haemolytic activity in comparison wit...
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PMID: 20093742
PDF is available here.
PDF is available here.
Abstract
Differentially expressed genes in peripheral blood mononuclear cells between ASCs and normal adults were isolated by suppression subtractive hybridization, and included some new genes. Of the upregulated genes in ASCs, checkpoint suppressor 1 is associated with DNA damage-induced cell cycle arrest....
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PMID: 19666407
PDF is available here.
PDF is available here.
Microbiology (155)Pt 8 2009
Abstract
Cells of wild-type Yersinia pestis exhibit a low-calcium response (LCR) defined as bacteriostasis with expression of a pCD-encoded type III secretion system (T3SS) during cultivation at 37 degrees C without added Ca(2+) versus vegetative growth with downregulation of the T3SS with Ca(2+) (>or=2.5 mM...
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PMID: 19443541
PDF is available here.
PDF is available here.
Abstract
We analyzed the killing of a large panel of melanoma cells by a high avidity, MART-1-specific T-cell clone or a MART-1-specific, polyclonal T-cell culture. Strikingly, we observed that the MART-1-specific T-cells only killed around half of the analyzed melanoma cell lines. In contrast a Bcl-2-specif...
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PMID: 18828018
PDF is available here.
PDF is available here.
Infection and Immunity (77)4 2009
Abstract
We report in this study that the pathogenesis patterns of plague infections caused by the deposition of 1- and 12-microm-particle aerosols of Y. pestis in the lower and upper respiratory tracts (URTs) of mice are different. The median lethal dose for 12-mum particles was 4.9-fold greater than that f...
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PMID: 19188359
PDF is available here.
PDF is available here.
Infection and Immunity (77)4 2009
Abstract
We evaluated the hypothesis that Ad-mediated delivery of a neutralizing antibody directed against the Y. pestis V antigen would protect mice against a Y. pestis challenge. MAbs specific for the Y. pestis V antigen were generated, and the most effective in protecting mice against a lethal intranasal...
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PMID: 19124600
PDF is available here.
PDF is available here.
AAPS Journal (11)1 2009
Abstract
Viruses deliver their genome into host cells where they subsequently replicate and multiply. A variety of relevant strategies have evolved by which viruses gain intracellular access and utilize cellular machinery for the synthesis of their genome. Therefore, the viral journey provides insight into t...
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PMID: 19194803
PDF is available here.
PDF is available here.
Abstract
We examined the effects of plant-derived F1, V, and F1-V on human cells of the innate immunity. F1, V, and F1-V proteins engaged TLR2 signalling and activated IL-6 and CXCL-8 production by monocytes, without affecting the expression of TNF-alpha, IL-12, IL-10, IL-1beta, and CXCL10. Native F1 antigen...
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PMID: 19309560
PDF is available here.
PDF is available here.
Abstract
We study the family of a child who died from HLH at 6 months of age due to a Q481P mutation in the perforin gene. The study is particularly interesting because the patient's heterozygous father experienced severe community-acquired pneumonia that could be attributed to deficient in vitro NK cell act...
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PMID: 19639728
PDF is available here.
PDF is available here.
Infection and Immunity (77)1 2009
Abstract
In this study, the binding of F components of the staphylococcal bicomponent leukotoxins Panton-Valentine leucocidin (LukF-PV) and gamma-hemolysin (HlgB) on polymorphonuclear neutrophils (PMNs), monocytes, and lymphocytes was determined using labeled mutants and flow cytometry. Leukotoxin activity w...
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PMID: 18838523
PDF is available here.
PDF is available here.
Infection and Immunity (76)12 2008
Abstract
We show that immunization with either purified rLcrV (a protein at the tip of type III needles) or a variant of this protein, recombinant V10 (rV10) (lacking amino acid residues 271 to 300), alone or in combination with rF1, prevented pneumonic lesions and disease pathogenesis. In addition, passive...
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PMID: 18794281
PDF is available here.
PDF is available here.
Abstract
We show that TLR6 associates with TLR2 to induce tolerogenic DC and regulatory type-1 T cells selectively secreting IL-10. In contrast, TLR1 heterodimerizes with TLR2 to promote proinflammatory IL-12p40 cytokine, producing DC and inflammatory T cell differentiation. LcrV specifically hijacks the TLR...
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PMID: 18854239
PDF is available here.
PDF is available here.
Journal of Molecular Biology (382)4 2008
Abstract
The mechanisms by which pore-forming toxins are able to insert into lipid membranes are a subject of the highest interest in the field of lipid-protein interaction. Eight mutants affecting different regions of sticholysin II, a member of the pore-forming actinoporin family, have been produced, and t...
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PMID: 18687335
PDF is available here.
PDF is available here.
Abstract
We previously reported that rat spleen T-cells and peripheral red blood cells that are deficient in glycosylphosphatidylinositol (GPI) synthesis [presumed mutants for the phosphatidylinositol glycan complementation group A gene (Pig-A)] could be detected by flow cytometry (FCM) as cells negative for...
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PMID: 18626996
PDF is available here.
PDF is available here.
Abstract
Membrane disruption can efficiently alter cellular function; indeed, pore-forming toxins (PFTs) are well known as important bacterial virulence factors. However, recent data have revealed that structures similar to those found in PFTs are found in membrane active proteins across disparate phyla. Man...
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PMID: 18778941
PDF is available here.
PDF is available here.
Infection and Immunity (76)10 2008
Abstract
These results show that IL-12 can be used as a molecular adjuvant to enhance protective immunity against pneumonic plague, but in a dose-dependent fashion....
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PMID: 18694965
PDF is available here.
PDF is available here.
Journal of Immunology (181)6 2008
Abstract
Although analysis of virus-specific CTL function at the peak of infection suggests that granzyme (grz) and perforin (pfp) gene expression is not coregulated, early differentiation events leading to acquisition of function are poorly understood. Using a combination of CFSE dilutions and single-cell R...
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PMID: 18768835
PDF is available here.
PDF is available here.
Plant Molecular Biology (68)1-2 2008
Abstract
We expressed the Yersinia pestis F1-V antigen fusion protein in tomato. We discovered that in some of these plants the expression of the f1-v gene was undetectable in leaves and fruit by ELISA, even though they had multiple copies of f1-v according to Southern-blot analysis. A likely explanation of...
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PMID: 18528764
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (283)35 2008
Oligomerization of PcrV and LcrV, protective antigens of Pseudomonas aeruginosa and Yersinia pestis.
Abstract
We demonstrate the assembly of V proteins into oligomeric doughnut-like complexes upon controlled refolding of the proteins in vitro. The oligomeric nature of refolded PcrV was revealed by size exclusion chromatography, native gel electrophoresis, and native mass spectrometry, which ascertain the ca...
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PMID: 18583342
PDF is available here.
PDF is available here.
Infection and Immunity (76)8 2008
Abstract
We report here expression of the plague F1-V fusion antigen in chloroplasts. Site-specific transgene integration and homoplasmy were confirmed by PCR and Southern blotting. Mature leaves showed the highest level of transgene expression on the third day of continuous illumination, with a maximum leve...
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PMID: 18505806
PDF is available here.
PDF is available here.