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Neuroendocrine Secretory Protein 7B2 (0):
Recent article
Neuroendocrine Secretory Protein 7B2
Abstract
We treated human (Caco-2BBe) and rat (IEC-6) intestinal epithelial cell lines with lymphotoxin beta receptor (LTbetaR) and TNF receptor (TNFR) agonists. Treated cells were studied for regulation of genes associated with M cell and follicle-associated epithelium (FAE). We found that LTbetaR and TNFR...
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PMID: 19501526
PDF is available here.
PDF is available here.
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Endocrinology (149)8 2008
Abstract
We here demonstrate that addition of exogenous recombinant 7B2 to CHO cells expressing pro-PC2 prevented the unfolding and aggregation of secreted PC2 forms in a dose-dependent manner, as assessed by aggregation assays, activity assays, cross-linking experiments, and sucrose density gradients. Intra...
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PMID: 18467442
PDF is available here.
PDF is available here.
Regulatory Peptides (148)1-3 2008
Abstract
Increased plasma concentrations were recorded in 11, 4 and 3 of the patients with the SgII 154-165 (N-terminal secretoneurin), the SgII 172-186 (C-terminal Secretoneurin) and the SgII 225-242 assays respectively. The SgIII, SgV, PC1/3 and PC2 assays failed to detect increased concentrations in any o...
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PMID: 18448176
PDF is available here.
PDF is available here.
Psychiatric Genetics (18)3 2008
Abstract
No significant difference was observed between the controls and patients in allelic frequencies or genotypic distributions of the 12 SNPs. In the analyses of the suggested five haplotypes, no significant difference between the controls and patients was observed in the distributions of any estimated...
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PMID: 18496211
PDF is available here.
PDF is available here.
Regulatory Peptides (146)1-3 2008
Abstract
We have studied the immunoreactivity of PC1/3, PC2 and furin in the four major NE cell types of the human pancreas by using double immunofluorescence techniques. The study also included the expression of NE secretory protein 7B2 (secretogranin V), a member of the granin family, which influences the...
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PMID: 17959263
PDF is available here.
PDF is available here.
BMC Genetics (9)1 2008
Abstract
Through a combination of DNA microarray analysis and quantitative PCR we identified a strong expression quantitative trait locus (eQTL) regulating Scg5 expression in two mouse chromosome 2 congenic strains and three additional F2 intercrosses. More importantly, the eQTL was coincident with a body we...
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PMID: 18439298
PDF is available here.
PDF is available here.
FEBS Letters (581)22 2007
Abstract
We analyzed wild-type and 7B2-null strains by HPLC and matrix-assisted laser desorption ionization time-of-flight mass spectrometry, which allowed the identification of a novel neuropeptide gene, flp-33. The presence and/or absence of some neuropeptides in 7B2-null animals strongly differs form the...
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PMID: 17707816
PDF is available here.
PDF is available here.
Abstract
We have identified a CpG island within the 5' region and untranslated first exon of the secretory granule neuroendocrine protein 1 gene (SGNE1/7B2) that showed hypermethylation in medulloblastomas compared to fetal cerebellum. Bisulfite sequencing and combined bisulfite restriction assay were perfor...
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PMID: 17334394
PDF is available here.
PDF is available here.
BMC Medical Genetics (8)1 2007
Abstract
We did not find any association between SGNE1 SNPs and childhood or adult obesity. However, the 5' region SNP -1,701A>G associated with higher area under glucose curve after oral glucose tolerance test (p = 0.0005), higher HOMA-IR (p = 0.005) and lower insulinogenic index (p = 0.0003) in obese child...
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PMID: 17617923
PDF is available here.
PDF is available here.
Abstract
We determined that 7B2 can be phosphorylated in Rin cells (a rat insulinoma cell line) and cultured chromaffin cells, but not in AtT-20 cells (derived from mouse anterior pituitary). Phosphoamino acid analysis of Rin cell 7B2 indicated the presence of phosphorylated serine and threonine. Phosphoryla...
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PMID: 16286464
PDF is available here.
PDF is available here.
Abstract
We examined whether this differential glucose homeostasis was associated with differences of proteolytic activation of pancreatic prohormones. Radioimmunoassays showed comparable levels of fasting plasma insulin between the two strains but a significantly lower glucagon level in B6 mice. Pulse-chase...
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PMID: 16443780
PDF is available here.
PDF is available here.
Abstract
We tested the hypothesis that proteomic analysis will identify protein biomarkers that provide insight into disease pathogenesis and are diagnostically useful. To identify ALS specific biomarkers, we compared the proteomic profile of cerebrospinal fluid (CSF) from ALS and control subjects using surf...
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PMID: 16313519
PDF is available here.
PDF is available here.
Endocrinology (146)11 2005
Abstract
We investigated the expression and potential role of the POMC system in human dermal papilla cells (DPCs), a specialized cutaneous mesenchymal cell type regulating hair follicle activity. In culture, these cells expressed POMC and displayed immunoreactivity for ACTH, alphaMSH, and beta-endorphin. Am...
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PMID: 16081629
PDF is available here.
PDF is available here.
Endocrinology (146)8 2005
Abstract
We have here examined strain influence on the hypothalamo-pituitary-adrenal (HPA) axis and glucose levels in wild-type, 7B2 null, and PC2 null mice. Wild-type B6 and 129 mice differed in basal corticosterone and glucose levels. When 7B2 nulls were transferred onto the B6 background, they survived an...
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PMID: 15878971
PDF is available here.
PDF is available here.
Endocrinology (146)2 2005
Abstract
We employed RT-PCR and immunohisto/cytochemistry, and a functional role for these POMC peptides was assessed in follicular melanocyte cultures. Human scalp hair follicle melanocytes synthesized and processed POMC. ACTH and alpha-MSH in association with their processing enzymes and MC-1R are expresse...
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PMID: 15498881
PDF is available here.
PDF is available here.
Abstract
We analyzed the gene expression profiles of FAE and M cells in order to characterize their cellular phenotypes. The microarray data revealed that, among approximately 14,000 genes, 409 were expressed in FAE at twofold or higher levels compared to the intestinal epithelial cells (IECs) of the villi....
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PMID: 16303744
PDF is available here.
PDF is available here.
Endocrinology (145)2 2004
Abstract
We have examined this proposition through the study of proopiomelanocortin (POMC) biosynthesis and granule content in both null models. RIA data indicate that both PC2 and 7B2 nulls lack pituitary alpha-MSH; interestingly, 7B2 nulls are still able to generate beta-endorphin from beta-lipotropin, whe...
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PMID: 14576186
PDF is available here.
PDF is available here.
Abstract
We studied the presence of autoantibodies against prohormone-processing enzymes, prohormone convertase (PC) 1/3, PC2, carboxypeptidase E (CPE), and PC2 regulatory protein, 7B2, by radioligand assay using recombinant human 35S-labeled protein in patients with clinically nonfunctioning pituitary macro...
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PMID: 14709807
PDF is available here.
PDF is available here.
Abstract
We used a cell line derived from a human pituitary adenoma (HP75) that has been shown to express 7B2, PC1, PC2, and TGFbeta receptors to analyze the effects of TGFbeta1 and the histone deacetylase inhibitor (HDACI) sodium butyrate (NaB) treatment on 7B2 mRNA expression along with the neuroendocrine-...
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PMID: 14709802
PDF is available here.
PDF is available here.
Abstract
Recent studies using biotechnological methods have achieved significant advances in our knowledge of molecular mechanisms underlying pituitary gland development and the differentiation of pituitary cytotypes. A large number of neuropeptides have been reported in the adult pituitary gland as well as...
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PMID: 12927577
PDF is available here.
PDF is available here.
Abstract
We have now examined the role of PC2 and its binding protein, 7B2, in the neuronal processing of proCCK by measurement of precursor, processing-intermediates and bioactive end-products in brain extracts from PC2- and 7B2-null mice and from corresponding controls. PC2-null mice displayed a nine-fold...
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PMID: 12472887
PDF is available here.
PDF is available here.
Journal of Endocrinology (175)3 2002
Abstract
The biosynthesis and processing of proinsulin was investigated in the diabetic Goto-Kakizaki (GK) rat. Immunofluorescence microscopy comparing GK and Wistar control rat pancreata revealed marked changes in the distribution of alpha-cells and pronounced beta-cell heterogeneity in the expression patte...
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PMID: 12475375
PDF is available here.
PDF is available here.
Abstract
In normal brain, secretogranin II and chromogranins A and S are extensively converted by endopeptidases to the peptides secretoneurin, GE-19, and PE-11, respectively. After genetic knockout of PC2 or its helper protein 7B2, but not after mutation of carboxypeptidase E, endoproteolytic processing dec...
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PMID: 12438088
PDF is available here.
PDF is available here.
Endocrinology (143)6 2002
Abstract
We investigated the endocrine and metabolic alterations of 7B2 null mice from pathological and biochemical points of view. Our results show that 7B2 nulls exhibit a multisystem disorder that includes severe pathoanatomical and histopathologic alterations of vital organs, including the heart and sple...
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PMID: 12021197
PDF is available here.
PDF is available here.
Endocrinology (143)6 2002
Abstract
We have investigated the effect of a single low dose of recombinant adenovirus vector encoding 7B2 and delivered directly to the pituitary of 7B2 nulls on pituitary ACTH, plasma ACTH, corticosterone, alpha MSH and glucose, and survival time. We show that after injection of recombinant adenovirus enc...
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PMID: 12021196
PDF is available here.
PDF is available here.
Abstract
We have compared the 7B2 and PC2 null mouse models to determine why the 7B2 null, but not the PC2 null, exhibits a lethal disease state. Both 7B2 and PC2 nulls contained highly elevated pituitary adrenocorticotropic hormone (ACTH); the neurointermediate lobe content of ACTH in 7B2 nulls was 13-fold...
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PMID: 11854475
PDF is available here.
PDF is available here.
Abstract
We first asked whether the N-terminal domain of proSAAS (proSAAS-(1-180)) could stabilize PC1 activity, similar to the effect of the N-terminal domain of 7B2 on PC2. Recombinant His-tagged proSAAS-(1-180) had no effect on PC1 activity in vitro and was unable to protect PC1 from thermal denaturation....
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PMID: 11719503
PDF is available here.
PDF is available here.
FEBS Letters (512)1-3 2002
Abstract
We introduced into mouse corticotroph AtT20 cells a retroviral vector carrying either a sense or an antisense 7B2 transgene to induce higher and lower 7B2 expression, respectively. Relative to control AtT20 cells, 7B2-overexpressing cells released less ACTH following KCl-induced membrane depolarizat...
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PMID: 11852092
PDF is available here.
PDF is available here.
Abstract
Expression of processed AVP in GC exposed patients was significantly decreased (p=0.021), while the amount of proAVP expression was unchanged. Despite the strong correlation between AVP and NP (the other cleavage product of proAVP) expression in the GC group (r=0.917, p=0.004), the mean NP immunorea...
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PMID: 11880860
PDF is available here.
PDF is available here.
FEBS Letters (510)1-2 2002
Abstract
We examined antral extracts from mice lacking PC2 or its chaperone, 7B2. The overall concentrations of precursors and alpha-amidated gastrins were similar in all mice. Chromatography, however, revealed that while the K(53)-K(54) site was almost fully cleaved in controls and half cleaved in PC2 null...
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PMID: 11755537
PDF is available here.
PDF is available here.
Abstract
We examined the role of the pentabasic site within 27-kDa 7B2 in this process. We prepared two His-tagged recombinant 7B2s by overexpression in bacteria: 7B2-Ser-Ser (SS), with an inactivating mutation in the CT peptide from Lys171-Lys172 (KK) to SS, rendering the CT peptide non-inhibitory; blockade...
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PMID: 11677272
PDF is available here.
PDF is available here.
Endocrinology (142)9 2001
Abstract
We have studied the expression of proSAAS and SPC3 mRNAs in the rat central nervous system and various peripheral tissues by in situ hybridization histochemistry. Our results show that, like 7B2, proSAAS is expressed with a panneuronal distribution. In the periphery, proSAAS is an excellent marker o...
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PMID: 11517193
PDF is available here.
PDF is available here.
Biochemical Journal (357)Pt 2 2001
Abstract
7B2 is an acidic protein residing in the secretory granules of neuroendocrine cells. Its sequence has been elucidated in many phyla and species. It shows high similarity among mammals. A Pro-Pro-Asn-Pro-Cys-Pro polyproline motif is its most conserved feature, being carried by both vertebrate and inv...
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PMID: 11439082
PDF is available here.
PDF is available here.
Abstract
Polypeptides in human cerebrospinal fluid (CSF), isolated by phase separation in chloroform-methanol-water and reversed-phase HPLC, were characterised by sequence analysis and mass spectrometry. This identified the presence of peptide fragments of testican, neuroendocrine specific protein VGF, neuro...
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PMID: 11339279
PDF is available here.
PDF is available here.
Abstract
Proopiomelanocortin peptides such as alpha-melanocyte-stimulating hormone and adrenocorticotropin are expressed in the epidermal and dermal compartment of the skin after noxious stimuli and are recognized as modulators of immune and inflammatory reactions. Human dermal microvascular endothelial cell...
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PMID: 11121136
PDF is available here.
PDF is available here.
Abstract
A general strategy is presented for the dominant negative reduction in the levels of heterodimeric soluble proteins within the secretory pathway through fusion of one of its partners C-terminal to the lysosomal enzyme cathepsin B (CB). Stable transfectants of CB-7B2 chimeras in AT20 cells result in...
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PMID: 11027518
PDF is available here.
PDF is available here.
Abstract
Abstract
We have investigated this in the embryonic avian pancreas, which is characterised by anatomical separation of insulin and glucagon islets. Moreover, we have compared the development of the endocrine cells to that of processing enzymes involved in pancreatic hormone biosynthesis. PYY-like immunoreact...
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PMID: 11052259
PDF is available here.
PDF is available here.
Abstract
Analysis of CCK content in extracts of whole forebrain from PC2 and 7B2 null mouse brain showed a significant decrease relative to wild-type brains. More detailed analysis revealed that CCK 8 amide levels in cerebral cortex and forebrain regions were more decreased than in hypothalamus. CCK 8 conten...
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PMID: 10873584
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (275)23 2000
Abstract
We searched the fly data base for the 7B2 signature motif PPNPCP and identified an expressed sequence tag clone encoding the entire open reading frame for this protein. dPC2 and d7B2 cDNAs were subcloned into expression vectors for transfection into HEK-293 cells; mPC2 and rat 7B2 were used as contr...
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PMID: 10749852
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (275)19 2000
Abstract
We compared its primary sequence with that of other PCs. This allowed us to distinguish a PC2-specific sequence at positions 242-248. We constructed two PC2 mutants in which residues 242 and 243 and residues 242-248 were replaced with the corresponding residues of PC1. Studies of in vivo cleavage of...
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PMID: 10799554
PDF is available here.
PDF is available here.
FEBS Letters (473)2 2000
Abstract
We here report evidence that 7B2 and proSAAS exhibit several elements of structural and functional homology. Firstly, 26 kDa human, mouse and rat proSAAS, like all vertebrate 7B2s, contain a proline-rich sequence within the first half of the molecule and also contain a C-terminal 40 residue peptide...
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PMID: 10812060
PDF is available here.
PDF is available here.
Abstract
We would like to propose that the pigmentation process may not necessarily involve a melanocortin-1 receptor-mediated mechanism....
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PMID: 10692100
PDF is available here.
PDF is available here.
Abstract
We use the P19 embryonal carcinoma cell line, which can differentiate into various cell types. The expression of convertase PC2 and its specific binding peptide 7B2 are co-induced during neuronal differentiation of P19 cells. We investigated the possibility that expression of both proteins may be co...
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PMID: 10793218
PDF is available here.
PDF is available here.
Abstract
We have analyzed the residues contributing to inhibition using N-terminal truncation and alanine scanning. Removal of more than 3 residues from the amino-terminal end of CT1-18 resulted in a more than 190-fold drop in inhibitory activity, showing that most of the residues between 3 and 18 are requir...
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PMID: 10673395
PDF is available here.
PDF is available here.
Molecular Brain Research (75)1 2000
Abstract
We used whole-mount in situ hybridization to visualize POMC mRNA expression in the intermediate and anterior pituitary of Xenopus tadpoles. A more sensitive analysis, namely semi-quantitative reverse-transcription polymerase chain reaction (RT-PCR) on total RNA isolated from Xenopus developmental st...
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PMID: 10648889
PDF is available here.
PDF is available here.
Molecular and Cellular Endocrinology (156)1-2 1999
Abstract
Processing of proproteins to biologically active peptides and, in the case of peptide hormones and neuropeptides, their sorting to granules of the regulated secretory pathway, requires the concerted action of a cascade of enzymes and chaperones. The purpose of this review is to summarize the recent...
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PMID: 10612417
PDF is available here.
PDF is available here.
The role of the 7B2 CT peptide in the inhibition of prohormone convertase 2 in endocrine cell lines.
Abstract
We constructed a mutant proenkephalin (PE) expression vector containing PE with its carboxyl-terminal peptide (peptide B) replaced with the 7B2 inhibitory CT peptide. This PECT chimera was stably transfected into two PC2-expressing cell lines, AtT-20/PC2 and Rin cells. Although recombinant PECT prov...
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PMID: 10461888
PDF is available here.
PDF is available here.
Abstract
We sought to block its expression in PC2-expressing endocrine cells using antisense strategies. We have previously shown that the endocrine rMTC 6-23 cell line expresses PC2 and that the enzyme is responsible for the processing of pro-neurotensin/neuromedin N (proNT/NN). Here, we show that rMTC 6-23...
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PMID: 10198237
PDF is available here.
PDF is available here.
Abstract
We created a null mutation in 7B2 employing a novel transposon-facilitated technique and compared the phenotypes of 7B2 and PC2 nulls. 7B2 null mice have no demonstrable PC2 activity, are deficient in processing islet hormones, and display hypoglycemia, hyperproinsulinemia, and hypoglucagonemia. In...
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PMID: 10089884
PDF is available here.
PDF is available here.
Abstract
We have characterized the proPC2 zymogen conversion process. Sequencing of the mature 66 kDa enzyme revealed a single site of cleavage at the paired basic site amino terminal to the GYRDI sequence. In contrast to mature PC2 activity, proPC2 conversion was inhibited neither by the eukaryotic subtilis...
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PMID: 9989936
PDF is available here.
PDF is available here.
Abstract
Mature peptide hormones and neuropeptides are typically synthesized from much larger precursors and require several posttranslational processing steps--including proteolytic cleavage--for the formation of the bioactive species. The subtilisin-related proteolytic enzymes that accomplish neuroendocrin...
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PMID: 10506829
PDF is available here.
PDF is available here.
DNA and Cell Biology (17)12 1998
Abstract
We first compared the distribution of 7B2 and PC2 mRNAs in the rat brain. Expression of 7B2 mRNA was found to be pan-neuronal, but additionally, we observed 7B2 mRNA in ependymal cells and in the subcommissural organ. Although the expression of PC2 mRNA was exclusively neuronal, it was more restrict...
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PMID: 9881669
PDF is available here.
PDF is available here.
Virchows Archiv (433)6 1998
Abstract
These results do not support a defect in expression or localization of proinsulin-processing enzymes in most insulinomas....
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PMID: 9870681
PDF is available here.
PDF is available here.
Abstract
We report a disturbance in VP precursor processing in the supraoptic and paraventricular nuclei of WS patients. In these patients with diabetes insipidus we could hardly detect any cellular immunoreactivity for processed VP in the supraoptic and paraventricular nuclei. On the other hand, in the para...
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PMID: 9814487
PDF is available here.
PDF is available here.
DNA and Cell Biology (17)8 1998
Abstract
We here report the cloning of the Caenorhabditis elegans 7B2 protein. Although weakly conserved with the vertebrate sequences (23% similarity with mouse 7B2), C. elegans 7B2 contains the signature PPNPCP motif as well as a highly conserved heptapeptide within the CT peptide. In in vitro assays, C. e...
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PMID: 9726255
PDF is available here.
PDF is available here.
Abstract
We have treated Xenopus intermediate pituitaries with the thiol-reducing agent dithiothreitol. Pulse-chase incubations in combination with immunoprecipitation analysis were used to monitor the fates of the prohormone proopiomelanocortin (POMC), prohormone convertase PC2 and its helper protein 7B2, a...
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PMID: 9648890
PDF is available here.
PDF is available here.
FEBS Letters (428)1-2 1998
Abstract
We hypothesized that some of these unique properties of PC2 are dictated by the presence of PC2-specific amino acids, which in the six other known mammalian convertases are otherwise conserved but distinct. Accordingly, six sites were identified within the catalytic segment of PC2. Herein we report...
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PMID: 9645470
PDF is available here.
PDF is available here.
Abstract
We show that a short segment in 7B2 (His120-Pro131) is important for its facilitative activity. Site-directed mutagenesis indicates that within this segment. Tyr130 is particularly important for the ability of 7B2 to act as a helper protein for PC2....
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PMID: 9607723
PDF is available here.
PDF is available here.
Abstract
We were able to show 7B2 immunoreactivity in the supraoptic nucleus (SON) or the paraventricular nucleus (PVN) in only three of five PWS patients. Here we report that in contrast with five other PWS patients, the neurons in the hypothalamic SON and PVN of the two 7B2-immunonegative PWS patients also...
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PMID: 9467579
PDF is available here.
PDF is available here.