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Nucleoproteins (6):
Recent article
Nucleoproteins
Abstract
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Nature Medicine (16)12 2010
Abstract
Abstract
We used cryo-electron microscopy to determine the architecture of nucleoprotein complexes composed of TFIID, TFIIA, the transcriptional activator Rap1 and yeast enhancer-promoter DNA. These structures revealed the mode of binding of Rap1 and TFIIA to TFIID, as well as a reorganization of TFIIA induc...
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PMID: 20559389
PDF is available here.
PDF is available here.
Abstract
We conducted genetic analyses. Phylogenetic analyses based on nucleoprotein (N) and hemagglutinin (H) gene sequences showed that these Menglian viruses were not closely related to sequences of any World Health Organization (WHO) reference strains representing the 23 currently recognized genotypes. T...
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PMID: 20507744
PDF is available here.
PDF is available here.
EMBO Reports (11)5 2010
Abstract
Abstract
We describe the recent advances that have been made by research into the role of TLR biology in host defense and disease....
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PMID: 20404851
PDF is available here.
PDF is available here.
Abstract
We speculate that the overall antibody-mediated inhibition of NMDARs expressed on GABAergic interneurons, glutamatergic neurons and dopaminergic neurons may cause neuropsychiatric symptoms and dyskinesias via dopamine and glutamate dysregulation. We also hypothesize that these antibodies affect not...
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PMID: 20420172
PDF is available here.
PDF is available here.
Abstract
We speculate that the overall antibody-mediated inhibition of NMDARs expressed on GABAergic interneurons, glutamatergic neurons and dopaminergic neurons may cause neuropsychiatric symptoms and dyskinesias via dopamine and glutamate dysregulation. We also hypothesize that these antibodies affect not...
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PMID: 20420172
PDF is available here.
PDF is available here.
Abstract
I and are of regional characteristic. The rabies viruses in high-incidence areas in China are of various origins and present the transmission tendency from high-incidence areas to surrounding regions. There may be cross-infection and mutual spread of rabies virus between wildlife and domestic animal...
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PMID: 21110419
PDF is available here.
PDF is available here.
Avian Diseases (54)1 Suppl 2010
Abstract
We have developed a multiplex fluorescence microsphere immunoassay (FMIA), which allows for the simultaneous detection and subtyping of chicken sera to H5 influenza viruses. The FMIA was developed using NP (full length) and H5 (HA1 region) proteins expressed in baby hamster kidney cells using a Vene...
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PMID: 20521712
PDF is available here.
PDF is available here.
Abstract
We show that BDV N assembles into ring- and string-like structures in the presence of 5' genomic BDV RNA. RNA induced polymerization is partly RNA-specific since polymerization is inefficient in the presence of 3' genomic BDV RNA or E. coli RNA. Mutagenesis of basic residues located in the cleft mad...
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PMID: 19945724
PDF is available here.
PDF is available here.
Abstract
Influenza virus causes acute respiratory disease in pigs and is of concern for its potential public health significance. Many subtypes of influenza virus have been isolated from pigs, and the virus continues to evolve in swine populations. Current antibody assays have limited antigenic recognition,...
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PMID: 20093676
PDF is available here.
PDF is available here.
Abstract
We used a genetic approach and constructed an Escherichia coli indicator strain to screen for random mutations in IntDOT that disrupt integrative recombination in vivo. Twenty-five IntDOT mutants were isolated and characterized for DNA binding, DNA cleavage, and DNA ligation activities. We found tha...
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PMID: 19915028
PDF is available here.
PDF is available here.
Mutation Research (704)1-3 2010
Abstract
Several proteins involved in DNA repair and DNA damage signaling have been shown to produce discrete foci in response to ionizing radiation. These foci are believed to co-localize to DSB and referred to as ionizing radiation-induced foci (IRIF) or DNA repair foci. Recent studies have revealed that s...
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PMID: 20096808
PDF is available here.
PDF is available here.
Cell Cycle (8)24 2009
Abstract
We have studied the effects of high levels of Polyhomeotic (PH) protein, a member of the Polycomb Group (PcG), during the proliferation of the imaginal discs in Drosophila. Overexpression of PH protein causes induction of proliferation, accompanied with induction of JNK-dependent apoptosis. As a res...
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PMID: 19923887
PDF is available here.
PDF is available here.
Abstract
A latex agglutination test (LAT) was developed for detecting antibodies against avian influenza virus. The recombinant avian influenza virus nucleoprotein expressed in Escherichia coli was purified, coupled with latex beads, and used as an antigen for the LAT. The LAT was capable of detecting anti-a...
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PMID: 19591876
PDF is available here.
PDF is available here.
Avian Pathology (38)5 2009
Abstract
We tested 24 stored avian brain samples, processed for histopathology and retained following their submission for necropsy or histopathology to the Schubot Exotic Bird Center diagnostic laboratory in 1992. Thirteen of these samples were from birds diagnosed at that time as suffering from PDD. The re...
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PMID: 19937526
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (284)28 2009
Abstract
We fused a hyperactive RecA protein (RecA803 protein) to monomeric red fluorescent protein (mRFP1) to produce a functional protein (RecA-RFP) that is suitable for in vivo and in vitro analysis. In vivo, the RecA-RFP partially restores UV resistance, conjugational recombination, and SOS induction to...
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PMID: 19419960
PDF is available here.
PDF is available here.
Bing du xue bao = Chinese journal of virology / [bian ji, Bing du xue bao bian ji wei yuan hui] (25)3 2009
Abstract
We reported. In order to supply the function necessary for rescuing and packaging of sendai virus vector in trans, the N, P, M, F, HN and L genes were separately cloned into an adenoviral shuttle expression vector pDC316 resulting in six recombinant adenoviral plasimds. Six replicating defective rec...
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PMID: 19634765
PDF is available here.
PDF is available here.
Abstract
We used RT-PCR to detect and characterize canine distemper virus isolated from 9 naturally infected foxes, 3 minks and 3 dogs in Poland by amplifying and sequencing a portion of the NP gene. A 293-bp fragment of the CDV NP gene was amplified by RT-PCR. Sequencing of the PCR products from the isolate...
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PMID: 20169915
PDF is available here.
PDF is available here.
Abstract
Abstract
We developed single-molecule fluorescence resonance energy transfer approaches to probe hRad52-mediated DNA annealing events in real time. We found that annealing proceeds in successive steps involving rearrangements of the ssDNA-hRad52 complex. Moreover, after initial pairing, further search for ex...
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PMID: 19074292
PDF is available here.
PDF is available here.
Emerging Infectious Diseases (14)12 2008
Enzootic rabies elimination from dogs and reemergence in wild terrestrial carnivores, United States.
Abstract
We studied RVs associated with recent and historic dog rabies enzootics worldwide. Molecular, phylogenetic, and epizootiologic evidence shows that domestic dog rabies is no longer enzootic to the United States. Nonetheless, our data suggest that independent rabies enzootics are now established in wi...
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PMID: 19046506
PDF is available here.
PDF is available here.
Abstract
Nucleic Acids Research (36)21 2008
Abstract
The C-terminal domains of Dnmt3a and Dnmt3L form elongated heterotetramers (3L-3a-3a-3L). Analytical ultracentrifugation confirmed the Dnmt3a-C/3L-C complex exists as a 2:2 heterotetramer in solution. The 3a-3a interface is the DNA-binding site, while both interfaces are essential for AdoMet binding...
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PMID: 18945701
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (283)46 2008
Abstract
Kinetoplast DNA (kDNA) is the mitochondrial DNA of trypanosomatids. Its major components are several thousand topologically interlocked DNA minicircles. Their replication origins are recognized by universal minicircle sequence-binding protein (UMSBP), a CCHC-type zinc finger protein, which has been...
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PMID: 18799461
PDF is available here.
PDF is available here.
Journal of General Virology (89)Pt 11 2008
Abstract
Human metapneumovirus (HMPV) is a recently discovered paramyxovirus of the subfamily Pneumovirinae, which also includes avian pneumovirus and human respiratory syncytial virus (HRSV). HMPV is an important cause of respiratory disease worldwide. To understand early events in HMPV replication, cDNAs e...
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PMID: 18931065
PDF is available here.
PDF is available here.
Abstract
We have mapped the loci in the NP, PA and M genes that determine the ca, ts and att phenotypes of MDV-B. In this report, the ts mechanism of MDV-B was described by comparing replication of MDV-B with its wild type counterpart at permissive and restricted temperatures. We showed that the PA and NP pr...
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PMID: 18804834
PDF is available here.
PDF is available here.
FASEB Journal (22)10 2008
Abstract
We report the 3.3-A crystal structure of H5N1 NP, which is composed of a head domain, a body domain, and a tail loop. Our structure resolves the important linker segments (residues 397-401, 429-437) that connect the tail loop with the remainder of the molecule and a flexible, basic loop (residues 73...
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PMID: 18614582
PDF is available here.
PDF is available here.
Abstract
These results were confirmed by co-immunoprecipitation studies showing an interaction between NP and nucleolin. In addition, in situ hybridization carried out with probes specific for each of the 8 RNA segments of ISAV showed that the genomic as well as the anti-genomic strands were also localized i...
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PMID: 18632128
PDF is available here.
PDF is available here.
EMBO Journal (27)16 2008
Abstract
We describe a structural and functional analysis of the HR inhibitor protein RecX and its mode of interaction with the RecA filament. RecX is a modular protein assembled of repeated three-helix motifs. The relative arrangement of the repeats generates an elongated and curved shape that is well suite...
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PMID: 18650935
PDF is available here.
PDF is available here.
Abstract
Peste des petits ruminants (PPR) is an important viral disease of sheep and goats, endemic in India. The study was undertaken to characterize the local PPRV by sequencing fusion (F) protein and nucleoprotein (N) gene segments and phylogenetic analysis, so as to focus on genetic variation in the fiel...
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PMID: 17850836
PDF is available here.
PDF is available here.
Abstract
To isolate the DNA fragments bound to cell-surface proteins with high affinity, peptide-DNA complexes were obtained by mild treatment of human endothelial cells with trypsin. After binding of nucleoprotein complexes to BrCN-Sepharose and extensive washing, 7-11 nt DNA fragments were eluted with acid...
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PMID: 18837924
PDF is available here.
PDF is available here.
Journal of Virology (82)16 2008
Abstract
We have evaluated the role of the internal proteins (N, P, and L) in NDV virulence by using a chimeric reverse-genetics approach. The N, P, and L genes were exchanged individually between an avirulent NDV strain, LaSota, and an intermediate virulent NDV strain, Beaudette C (BC), and the N and P gene...
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PMID: 18550657
PDF is available here.
PDF is available here.
Experimental Animals (57)4 2008
Abstract
We can exactly check the LCMV contamination in laboratory animals....
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PMID: 18633158
PDF is available here.
PDF is available here.
Virus Research (135)1 2008
Abstract
We determined and analyzed 1307 nucleotides of nucleoprotein genes of 34 rabies field isolates collected from Sumatra, Java, Kalimantan, Sulawesi and Flores islands. Results of phylogenetic analysis indicated that rabies isolates in Indonesia formed one cluster, were of Asian lineage, and were close...
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PMID: 18420300
PDF is available here.
PDF is available here.
Virus Research (135)1 2008
Abstract
Lagos bat virus (LBV) belongs to genotype 2 of the Lyssavirus genus. The complete nucleoprotein (N), phosphoprotein (P), matrixprotein (M) and glycoprotein (G) genes of 13 LBV isolates were sequenced and phylogenetically compared with other lyssavirus representatives. The results identified three di...
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PMID: 18359532
PDF is available here.
PDF is available here.
Journal of Virology (82)13 2008
Abstract
We show that two domains of this protein determine this aberrant migration and that this region mediates its incorporation into virions. These regions, amino acids 439 to 492 and amino acids 589 to 739, alter the mobility of Ebola virus NP by sodium dodecyl sulfate-polyacrylamide gel electrophoresis...
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PMID: 18417588
PDF is available here.
PDF is available here.
Abstract
We develop a conformational model of this type of protein, using an explicit description of the unfolded state, specifically modified to allow for the presence of transient secondary structure, and combining this with extensive measurement of residual dipolar couplings throughout the chain. This com...
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PMID: 18507376
PDF is available here.
PDF is available here.
Journal of General Virology (89)Pt 6 2008
Abstract
The Borna disease virus (BDV) proteins X and P are translated from a bicistronic viral mRNA. Here, it was shown that the rescue of recombinant BDV from cDNA was enhanced approximately eightfold if reconstitution of the viral polymerase complex was performed with an expression vector encoding X and P...
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PMID: 18474560
PDF is available here.
PDF is available here.
Abstract
Abstract
We report the first identification of phosphorylation sites of the nucleoprotein (N) of the family Paramyxoviridae. The N protein is known to be the most abundant protein in infected cells; it constructs the N-RNA complex (nucleocapsid) and supports transcription and replication of viral genomic RNA...
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PMID: 18442172
PDF is available here.
PDF is available here.
Abstract
We have characterized a ribonucleoprotein (RNP) complex consisting of the TGB1 protein and plus-sense BSMV RNAs from infected barley plants and have identified TGB1 complexes in planta and in vitro. Homologous TGB1 binding was disrupted by site-specific mutations in each of the first two N-terminal...
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PMID: 18353960
PDF is available here.
PDF is available here.
Bing du xue bao = Chinese journal of virology / [bian ji, Bing du xue bao bian ji wei yuan hui] (24)3 2008
Abstract
Paramyxovirus Tianjin strain is a novel strain of virus causing common cotton-eared marmoset fatal infection. To investigate the relationship between the gene structure and function of nucleoprotein (NP) of Tianjin strain, NP gene of paramyxovirus Tianjin strain was cloned and three domains of NP we...
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PMID: 18683562
PDF is available here.
PDF is available here.
Abstract
These results indicate that IVpi-189 virus, an avirulent temperature-sensitive mutant, is a promising candidate for use as a live-attenuated vaccine....
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PMID: 18360902
PDF is available here.
PDF is available here.
Journal of Virology (82)9 2008
Abstract
We used reverse genetics to generate two H5N1 recombinant viruses with differing pathogenicity in chickens. Single-gene reassortants were used to determine which viral genes contribute to the altered virulence. Exchange of the PB1, PB2, and NP genes impacted replication of the reassortant viruses wh...
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PMID: 18305037
PDF is available here.
PDF is available here.
Abstract
We developed an adenoviral vector-based vaccine that contains hemagglutinin protein from clade 1 and clade 2 viruses, as well as conserved nucleoprotein, to broaden the vaccine coverage against H5N1 viruses....
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PMID: 18462165
PDF is available here.
PDF is available here.
Abstract
We provide evidence that this functional divergence is driven by positive selection. Based on resequencing of an approximately 30-kb region around the ph locus in an African sample of D. melanogaster X chromosomes, we identified a selective sweep, estimated its age and the strength of selection, and...
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PMID: 18381818
PDF is available here.
PDF is available here.
Abstract
In rabies endemic countries, funds and infrastructure are often insufficient to employ the approved gold standard for the definitive diagnosis of rabies: the direct fluorescent test. In the present study, two types (type 1 and 2) of an ICT kit were evaluated for detection of rabies. These were devel...
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PMID: 18426399
PDF is available here.
PDF is available here.
Journal of General Virology (89)Pt 4 2008
Abstract
We tested a panel of PPMO designed to target conserved sequences in the RNA genome segments encoding polymerase subunits of a highly pathogenic mouse-adapted influenza A virus (SC35M; H7N7). Three PPMO, targeting the translation start site region of PB1 or NP mRNA or the 3'-terminal region of NP vir...
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PMID: 18343835
PDF is available here.
PDF is available here.
Journal of Virology (82)7 2008
Abstract
We analyzed a range of influenza A virus strains for their sensitivities to murine Mx1 and human MxA proteins and found remarkable differences. Virus strains of avian origin were highly sensitive to Mx1, whereas strains of human origin showed much weaker responses. Artificial reassortments of the vi...
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PMID: 18199636
PDF is available here.
PDF is available here.
Molecular Cell (29)5 2008
Abstract
We have characterized glucocorticoid receptor (GR) binding events and chromatin structural transitions across GR-induced or -repressed genes. This analysis reveals that GR binding invariably occurs at nuclease-accessible sites (DHS). A remarkable diversity of mechanisms, however, render these sites...
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PMID: 18342607
PDF is available here.
PDF is available here.
Abstract
We explored the molecular mechanisms underlying this complex mode of regulation by examining the nucleoprotein structure of the CD4 locus throughout T cell development and in DN cells lacking the CD4 silencer. In DN cells, the CD4 enhancer is preloaded with multiple transcription activators, but p30...
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PMID: 18322012
PDF is available here.
PDF is available here.
Abstract
We investigate the effect of ISG15 protein on the release of Ebola VP40 VLPs. ISG15 is an IFN-inducible, ubiquitin-like protein expressed after bacterial or viral infection. Our results show that expression of free ISG15, or the ISGylation system (UbE1L and UbcH8), inhibits budding of Ebola virus VP...
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PMID: 18305167
PDF is available here.
PDF is available here.
Abstract
The S-layer protein CTC surface display system of Bacillus thuringiensis was used to test the possibility of displaying avian influenza virus nucleoprotein (NP) on the cell surface of B. thuringiensis. By fusing np with the anchoring motif of ctc, four recombinant plasmids were constructed. They har...
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PMID: 18236038
PDF is available here.
PDF is available here.
Abstract
A cell line of Madin-Darby canine kidney (MDCK) cells persistently infected with human influenza A virus has been established and designated as MDCK-IVpi cells. Production of progeny virus in MDCK-IVpi cells was suppressed when the cells were incubated in the presence of 10% fetal calf serum (FCS)....
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PMID: 17611773
PDF is available here.
PDF is available here.
Journal of Immunology (180)5 2008
Abstract
We have shown previously that the kinetics of Ag presentation determine immunodominance of the LCMV-specific CTL response due to progressive exhaustion of LCMV nucleoprotein (NP)-specific CTL upon increasing viral load. In this study, we provide evidence that CTL against early LCMV NP-derived epitop...
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PMID: 18292534
PDF is available here.
PDF is available here.
Abstract
Rapid and accurate diagnosis of avian influenza (AI) infection is important for an understanding epidemiology. In order to develop rapid tests for AI antigen and antibody detection, two monoclonal antibodies (mAbs) against influenza nucleoprotein (NP) were produced. These mAbs are designated as F26-...
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PMID: 18006085
PDF is available here.
PDF is available here.
Antiviral Research (77)2 2008
Abstract
We demonstrate that treatment of host cells with the tyrosine kinase inhibitor genistein inhibits infection of cells with the New World arenavirus Pichindé (PICV). The greatest degree of inhibition was observed in pre-treated target cells, but modest inhibition of infection was also seen when drug...
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PMID: 17961732
PDF is available here.
PDF is available here.
Journal of Molecular Biology (375)4 2008
Abstract
The human papillomavirus (HPV) DNA replication origin (ori) shares a common theme with many DNA control elements in having multiple binding sites for one or more proteins spaced over several hundreds of base pairs. The HPV type 11 ori spans 103 bp and contains three palindromic E2 binding sites (E2B...
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PMID: 18067922
PDF is available here.
PDF is available here.