Abstract
Many filamentous fungi secrete considerable quantities of enzymes including protease, cellulase and xylanase, which are of major industrial importance. Over the past few decades, many of these fungal enzymes have been isolated and their relevant genes characterised. Solid-state fermentation (SSF), a...
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PMID: 20145973
PDF is available here.
Maurizio Bevilacqua,
Ligia J Dominguez,
Velella Righini,
Tarcisio Vago,
Diego Foschi,
Fabio Corsi,
Emilio Trabucchi,
Enrica Chebat,
Maria Del Carmen Borghi,
Gian Marco Diana,
Massimo Barrella and
Mario Barbagallo
Abstract
Before RYGB, peptones increased gastrin (P < .001), and decreased iCa (P < .01) without changes in PTH or pH. Both phosphate and PTH markedly increased after RYGB with the peptones oral load (P < .01), without changes in pH, iCa, or gastrin. There was a significant, direct relationship between the i...
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PMID: 20403518
PDF is available here.
Abstract
As a result of screening Bacillus sp. strains isolated from different natural substrates, strain BKL20 was identified as a producer of a thermostable alkaline alpha-amylase. Maximum production of this alpha-amylase was achieved by optimizing culture conditions. Production of alpha-amylase seemed to...
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PMID: 20453894
PDF is available here.
Abstract
A Janus head-like bi-cathodic microbial fuel cell was constructed to monitor the electron transfer from Saccharomyces cerevisiae to a woven carbon anode. The experiments were conducted during an ethanol cultivation of 170 g/l glucose in the presence and absence of yeast-peptone mediu...
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PMID: 19946948
PDF is available here.
Abstract
A Janus head-like bi-cathodic microbial fuel cell was constructed to monitor the electron transfer from Saccharomyces cerevisiae to a woven carbon anode. The experiments were conducted during an ethanol cultivation of 170 g/l glucose in the presence and absence of yeast-peptone mediu...
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PMID: 19946948
PDF is available here.
Abstract
A Janus head-like bi-cathodic microbial fuel cell was constructed to monitor the electron transfer from Saccharomyces cerevisiae to a woven carbon anode. The experiments were conducted during an ethanol cultivation of 170 g/l glucose in the presence and absence of yeast-peptone mediu...
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PMID: 19946948
PDF is available here.
Abstract
A Janus head-like bi-cathodic microbial fuel cell was constructed to monitor the electron transfer from Saccharomyces cerevisiae to a woven carbon anode. The experiments were conducted during an ethanol cultivation of 170 g/l glucose in the presence and absence of yeast-peptone mediu...
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PMID: 19946948
PDF is available here.
Abstract
A Janus head-like bi-cathodic microbial fuel cell was constructed to monitor the electron transfer from Saccharomyces cerevisiae to a woven carbon anode. The experiments were conducted during an ethanol cultivation of 170 g/l glucose in the presence and absence of yeast-peptone mediu...
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PMID: 19946948
PDF is available here.
Abstract
Few strains of Metschnikowia pulcherrima (Pitt) M.W. Miller are under development for control of postharvest pathogens on fruit. A substrate was developed to optimize the biomass production of M. pulcherrima strain BIO126. Different complex nutrient sources, with or without pH control, were tested....
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PMID: 20237574
PDF is available here.
Abstract
The study evaluated the response of an enriched microbial culture on 2,6-dihydroxybenxoic acid (2,6-DHBA) and peptone mixture at low sludge age (theta(X)) under aerobic conditions. It emphasized the effect of culture history by comparing the response of the microbial culture sustained at identical c...
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PMID: 20419584
PDF is available here.
Abstract
This work aims to describe the effect of the feeding regime of anaerobic activity tests on the limit viscosity (mu(lim)) evolution of the granules. Batch experiments were performed with 3 different sources of substrate: acetate, peptone, and glucose. Despite, the substrate origin was shown to affect...
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PMID: 20595763
PDF is available here.
Abstract
Our results suggest that by using tryptone alone as an amino acid source, pDNA amplification was improved and a specific yield of 20.43 mg pDNA/g dcw was achieved, proving that this strategy can improve pDNA yield even at a small scale....
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PMID: 19996695
PDF is available here.
Abstract
For several years in the Quebec fisheries' industry, landings of pelagic fish have been calculated at over 4000 tons. These under-exploited species, rich in lipids and proteins, could be used in valuable new products. In the present study, hydrolysates of mackerel and herring were produced and utili...
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PMID: 19940932
PDF is available here.
Abstract
BconP dose-dependently induced CCK secretion in STC-1 cells. Treatment with BAPTA-AM, an intracellular Ca2+ chelator, reduced BconP-induced CCK secretion, however, removal of extracellular Ca2+ did not affect the secretory response. Treatment with 2-amino borate (2-APB) reduced CCK releasing respons...
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PMID: 19099240
PDF is available here.
Abstract
Extracellular amylase production by a newly isolated alkali-thermotolerant strain Streptomyces gulbargensis DAS 131 was optimized and characterized. The highest amylase production was achieved by growing S. gulbargensis DAS 131 in media with 1% starch. Strain exhibited maximal activity at pH 9.0 and...
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PMID: 18846397
PDF is available here.
Abstract
Rapid advancements in biotechnology depend on the development of reliable sensing sytems adaptable to a variety of analytes. Molecularly imprinted polymers combined with mass-sensitive devices (10 MHz QCMs) provide a reliable and robust method to observe cell growth and allow cell specifications in...
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PMID: 19173063
PDF is available here.
Abstract
The aim of the present study was to improve the sanitary quality of in vitro-produced bovine embryos by using plant protein hydrolysates (plant peptones) as substitutes for animal proteins. Peptones were compared with bovine serum albumin (BSA) as the protein source in synthetic oviduct fluid medium...
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PMID: 19383265
PDF is available here.
Abstract
The continuous aerobic degradation of phenol, mixed with readily degradable synthetic wastewater was studied over a period of 400 days at 25+/-5 degrees C temperature in a fixed bed biofilm reactor using 'Liapor' clay beads as packing material. The phenol concentration added to the reactor ranged fr...
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PMID: 18440804
PDF is available here.
Abstract
Statistics based experimental designs were used to optimize the medium for antifungal active substances production from a newly isolated Paenibacillus polymyxa Cp-S316 in shaker flask cultivation. The medium components having significant effect on the production were first identified using a fractio...
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PMID: 18448333
PDF is available here.
Abstract
Current study was focused on the development of a non-fastidious lactic acid producing strain having better growth rate, low pH tolerance and good productivity by genome shuffling of a mutant strain of Lactobacillus delbrueckii NCIM 2025 and an amylase producing non-fastidious Bacillus amyloliquefac...
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PMID: 18482834
PDF is available here.
Abstract
Effects of medium components and culture conditions on biomass and lipid production of Trichosporon fermentans were studied. The optimal nitrogen source, carbon source and C/N molar ratio were peptone, glucose and 163, respectively. The favorable initial pH of the medium and temperature were 6.5 and...
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PMID: 18394882
PDF is available here.
Abstract
Addition of nitrogen sources as supplementary nutrient into MSM medium to enhance biodegradation by stimulating the growth four isolates, Acinetobacter faecalis, Staphylococcus sp., Pseudomonas putida and Neisseria elongata isolated from petroleum contaminated groundwater, wastewater aeration pond a...
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PMID: 19266926
PDF is available here.
Abstract
Six acetic acid bacterial isolates, obtained during a study of the microbial diversity of spontaneous fermentations of Ghanaian cocoa beans, were subjected to a polyphasic taxonomic study. (GTG)(5)-PCR fingerprinting grouped the isolates together, but they could not be identified using this method....
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PMID: 18768626
PDF is available here.
Abstract
The efe gene encoding an ethylene-forming enzyme from Pseudomonas syringae pv. glycinea has been expressed for the first time under the control of Trichoderma reesei cbh1 promoter in Trichoderma viride. Reverse transcription polymerase chain reaction analysis showed that transformant Y2 produced mRN...
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PMID: 18575855
PDF is available here.
Abstract
The strategy of optimization using sequential factorial design was employed to enhance the tensio-active emulsifying agent produced by Candida lipolytica using soybean oil refinery residue as substrate. A full factorial design was used to evaluate the impact of three fermentation factors-amounts of...
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PMID: 18563463
PDF is available here.
Abstract
Improved titres of hirsutellones in H. nivea BCC 2549 were achieved with an agitated (200 rev min(-1)) fructose-peptone medium at 25 degrees C. SIGNIFICANCE AND IMPACT OF THE STUDY: Improved yields of hirsutellones B-D will enable medicinal chemistry modifications leading to a development of a poten...
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PMID: 18565141
PDF is available here.
Abstract
The effects of cultivation medium compositions including soybean meal, peptone, soybean oil and cornstarch for actinomycin X2 production by Streptomyces spp JAU4234 were accessed by using response surface methodology. The 2(4) full factorial designs and the paths of steepest ascent were effective in...
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PMID: 18347826
PDF is available here.
Abstract
We previously demonstrated that Streptococcus oligofermentans suppressed the growth of Streptococcus mutans, the primary cariogenic pathogen, by producing hydrogen peroxide (H(2)O(2)) through lactate oxidase activity. In this study, we found that the lox mutant of S. oligofermentans regained the inh...
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PMID: 18469105
PDF is available here.
Abstract
Rhizopus chinensis CCTCC M201021 was a versatile strain capable of producing whole-cell lipase with synthetic activity in submerged fermentation. In order to improve the production of whole-cell lipase and study the culture conditions systematically, the combination of taguchi method and response su...
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PMID: 17888652
PDF is available here.
Abstract
We investigated the production of biofungicide phenazine-1-carboxlic (PCA) by Pseudomonas sp. M18G, a gacA-deficient mutant of M18 for PCA high-production. Glucose was chosen as the optimal carbon source and soy peptone as the nitrogen source. A Plackett-Burman design revealed that glucose, soy pept...
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PMID: 18078294
PDF is available here.
Abstract
Sorption of three types of dissolved organic matter (DOM; i.e., humic acid, peptone and alpha-phenylalanine) by a mutiwalled carbon nanotube (MWNT40) and sorption of phenanthrene (Phen), naphthalene (Naph), and 1-naphthol (1-Naph) by the original and DOM-coated MWNT40 were examined. Sorption data of...
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PMID: 18522095
PDF is available here.
Abstract
Aspergillus niger EBK-9 was isolated from soil sample. This isolate was evaluated for production of (R)-1-(4-Bromo-phenyl)-ethanol 2 from 1-(4-Bromo-phenyl)-ethanone 1. In this work, the production of the 2 was achieved via fermenter. Glucose, yeast extract and ram horn peptone as medium in fermente...
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PMID: 17531472
PDF is available here.
Abstract
Nutrition had highly influence on the antibiotic production by Xenorhabdus nematophila YL001. Glucose and peptone were identified as the best carbon and nitrogen sources that significantly affected antibiotic production using one-factor-at-a-time approach. Response surface methodology was applied to...
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PMID: 17531470
PDF is available here.
Abstract
The optimal flask-shaking batch fermentation medium for phenazine-1-carboxylic acid (PCA) production by Pseudomonas sp. M-18Q, a qscR chromosomal inactivated mutant of the strain M18 was studied using statistical experimental design and analysis. The Plackett-Burman design (PBD) was used to evaluate...
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PMID: 18397774
PDF is available here.
Abstract
Variation in peptone as a constituent of BPW has a significant effect on growth and enumeration of bacteria. SIGNIFICANCE AND IMPACT OF THE STUDY: Increased consideration with respect to culture media may significantly improve bacterial growth and experimental reproducibility....
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PMID: 18194259
PDF is available here.
Abstract
The behavior of Penicillium camembertii and Geotrichum candidum growing in submerged pure cultures on simple (glutamate) or complex (peptones) substrates as nitrogen and carbon sources and an lactate as a second carbon source was examined. Similar to the behavior previously recorded on a simple subs...
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PMID: 18239428
PDF is available here.
Abstract
A novel anaerobic, mesophilic, protein-utilizing bacterial strain, GW(T), was isolated from the mesophilic hydrogen-producing granular sludge used to treat food industry wastewater. The strain was a Gram-positive, non-spore-forming and non-motile rod. Growth of the strain was observed at 20-48 degre...
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PMID: 18175674
PDF is available here.
Abstract
The preferential utilization of different electron donors and their effects on the nitrate reduction and methanogenesis in a mixed, mesophilic (35 degrees C) methanogenic culture were investigated. Batch methanogenic cultures were fed with dextrin/peptone (D/P), propionate, acetate, and H(2)/CO(2) a...
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PMID: 18401140
PDF is available here.
Abstract
Low-temperature, long-time (LTLT) pasteurization assures the safety of banked human milk; however, heat can destroy important nutritional biomolecules. High-pressure processing (HPP) shows promise as an alternative for pasteurization of breast milk. The purpose of this study was to investigate the e...
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PMID: 18236670
PDF is available here.
Abstract
We demonstrated the feasibility of nuclear magnetic resonance (NMR) spectroscopy in combination with chemometrics in rapid screening peptone lots in order to optimize efficiency and consistency of large-scale protein production. This report is the first that shows a correlation between the intrinsic...
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PMID: 17274067
PDF is available here.
Abstract
Two thermophilic continuously stirred tank reactors, R1 and R2, were subject to pulses of tryptone and ammonia. R1 was operated at an ammonia-N concentration of 3.0 g l(-1) and R2 was operated at an ammonia-N concentration of 1.7 g l(-1). Shock loads of tryptone (10 g l(-1), 10 g l(-1), 15 g l(-1))...
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PMID: 17879849
PDF is available here.
Abstract
Starch industry wastewater was investigated to assess and improve its potential as a raw material for the conidia production of biocontrol fungi, Trichoderma viride. The wastewater was tested with and without supplements of glucose, soluble starch, meat peptone and probable conidiation inducer chemi...
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PMID: 17084079
PDF is available here.
Abstract
We conclude that strain OGL-20P(T) represents a new separate species within the genus Thermococcus, for which we propose the name Thermococcus thioreducens sp. nov. The type strain is OGL-20P(T) (=JCM 12859(T)=DSM 14981(T)=ATCC BAA-394(T))....
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PMID: 17625204
PDF is available here.
Abstract
Molasses spent wash from cane-molasses based distilleries contains a brown coloured recalcitrantpolymer melanoidin, which if disposed untreated poses a great threat to environment. Microbial decolorization and chemical oxygen demand (COD) reduction was found to be dependent on specific carbon and ni...
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PMID: 18380094
PDF is available here.
Abstract
We investigated the effectiveness of peptones from a variety of sources on the sporulation of this organism compared with the peptone in the original formulation, proteose peptone (control). Seven strains were used to screen 32 peptones, with starch or raffinose as the carbohydrate source. In most c...
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PMID: 17685351
PDF is available here.
Abstract
These results will be useful to the development of improved reference materials and samples held in culture collections....
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PMID: 17482158
PDF is available here.
Abstract
A pink-pigmented, phototrophic, purple nonsulfur bacterium, strain JA173(T), was isolated in pure culture from a saltern in Gokarna, India, in a medium containing 2 % (w/v) NaCl. Strain JA173(T) was a non-motile Gram-negative rod that multiplied by budding. Phylogenetic analysis based on 16S rRNA ge...
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PMID: 17473235
PDF is available here.
Abstract
We discovered that the upregulation of CCK transcription involves ERK1/2, PKA, and calmodulin-dependent protein kinase-mediated pathways. Additionally, GPR93 activation by peptone induces a response in CCK release at 15 min, which continues over a 2-h period. The cAMP level in STC-1 cells overexpres...
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PMID: 17290006
PDF is available here.
Abstract
A statistical approach response surface methodology (RSM) was used to study the production of succinic acid from Bacteroides fragilis. The most influential parameters for succinic acid production obtained through one-at-a-time method were glucose, tryptone, sodium carbonate, inoculum size and incuba...
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PMID: 17303449
PDF is available here.
Abstract
We reported that a sponge-associated fungus, Letendraea helminthicola, produced two antifouling compounds: 3-methyl-N-(2-phenylethyl) butanamide and cyclo(D-Pro-D-Phe). To optimize the production of these antifouling compounds, we then examined the production of compounds under different culture con...
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PMID: 17216446
PDF is available here.
Abstract
Broiler retail samples (n=113) were analyzed to determine (i) the effectiveness of buffered peptone water (BPW) supplemented with blood and antibiotics for the isolation of Campylobacter jejuni and C. coli, (ii) if a 1:4 enrichment ratio performs similarly as a 1:9 ratio, and (iii) if BPW is similar...
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PMID: 17257696
PDF is available here.
Abstract
Growth and genetic stability of Nicotiana tabacum L. callus were strongly improved by replacing the inorganic nitrogen and phosphorus of the Murashige and Skoog's medium by a soybean peptone and phytate, respectively. Cell proliferation after subcultivation on the modified medium was highly stimulat...
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PMID: 16972097
PDF is available here.
Abstract
Concentrations of Mg(2+), glycine, yeast extract, biotin, acetaldehyde and peptone were optimized by a uniform design process for ethanol production by Saccharomyces cerevisiae. Using non-linear step-wise regression analysis, a predictive mathematical model was established. Concentrations of Mg(2+)...
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PMID: 17091376
PDF is available here.
Abstract
We have previously found that inhibition of PCs by tryptone occurs only in gametes that display chemotaxis toward this agent, and is not observed in chemotactically insensitive vegetative cells. Here we show that the sensitivity to tryptone is characteristic of gametes of both mating types, and exam...
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PMID: 16896790
PDF is available here.
Abstract
Principles and procedure for rapid estimation of bacteria susceptibility to antibiotics on a glucose-tryptone medium with an indicator are described. The results of the tests with 50 microbial strains of 17 species showed practically complete identity to the results of the antibiograms when the data...
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PMID: 18461804
PDF is available here.
Abstract
A simple and low-cost procedure was developed for the effective processing of native calf skin and blood wastes to produce protein hydrolysates. The method includes extraction of high-molecular-weight protein from the raw material, followed by enzymatic hydrolysis of the extracted residue. The enzym...
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PMID: 17171464
PDF is available here.
Abstract
Different concentrations of sucrose (3-25% w/v) and peptone (2-5% w/v) were studied in the formulation of media during the cultivation of Aspergillus japonicus-FCL 119T and Aspergillus niger ATCC 20611. Moreover, cane molasses (3.5-17.5% w/v total sugar) and yeast powder (1.5-5% w/v) were used as al...
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PMID: 16835781
PDF is available here.
Abstract
In this study, an organic solvent tolerant bacterial strain was isolated. This strain was identified as Pseudomonas sp. strain S5, and was shown to degrade BTEX (Benzene, Toluene, Ethyl-Benzene, and Xylene). Strain S5 generates an organic solvent-tolerant lipase in the late logarithmic phase of grow...
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PMID: 17205035
PDF is available here.