Karsten Klopffleisch,
Nguyen Phan,
Kelsey Augustin,
Robert S Bayne,
Katherine S Booker,
Jose R Botella,
Nicholas C Carpita,
Tyrell Carr,
Jin-Gui Chen,
Thomas Ryan Cooke,
Arwen Frick-Cheng,
Erin J Friedman,
Brandon Fulk,
Michael G Hahn,
Kun Jiang,
Lucia Jorda,
Lydia Kruppe,
Chenggang Liu,
Justine Lorek,
Maureen C McCann,
Antonio Molina,
Etsuko N Moriyama,
M Shahid Mukhtar,
Yashwanti Mudgil,
Sivakumar Pattathil,
John Schwarz,
Steven Seta,
Matthew Tan,
Ulrike Temp,
Yuri Trusov,
Daisuke Urano,
Bastian Welter,
Jing Yang,
Ralph Panstruga,
Joachim F Uhrig and
Alan M Jones
Abstract
We screened a set of proteins from the G-protein complex using two-hybrid complementation in yeast. After deep and exhaustive interrogation, we detected 544 interactions between 434 proteins, of which 68 highly interconnected proteins form the core G-protein interactome. Within this core, over half...
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PMID: 21952135
PDF is available here.
Abstract
We explore the stromal proteome of spinach and Arabidopsis chloroplast and show that clustering of mass spectra is a useful tool for investigating such variants and detecting modified peptides with amino acid substitutions or post-translational modifications. This study employs data mining by hierar...
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PMID: 21539947
PDF is available here.
Abstract
We have developed an assay in which the interaction between F. graminearum and the model plant Arabidopsis thaliana is monitored in liquid media in 96-well plates. In this assay, fungal infection is associated with the development of dark lesion-like spots on the cotyledons of Arabidopsis seedlings...
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PMID: 21303209
PDF is available here.
Abstract
The flowering plant germline is produced during the haploid gametophytic stage. Defining the germline is complicated by the extreme reduction of the male and female gametophytes, also referred to as pollen and embryo sac, respectively. Both male and female gamete progenitors are segr...
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PMID: 21332359
PDF is available here.
Abstract
We show through chromatin immunoprecipitation (ChIP) that SPT5L/KTF1, a silencing factor and a homolog of SPT5 elongation factors, binds chromatin at loci subject to transcriptional silencing. Chromatin binding of SPT5L/KTF1 occurs downstream of RNA Polymerase V, but independently from the presence...
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PMID: 21738482
PDF is available here.
Abstract
We use genetics and live imaging to examine the cell biology of CLV1 in Arabidopsis meristematic tissue. We demonstrate that plasma membrane-localized CLV1 is reduced in concentration by CLV3, which causes trafficking of CLV1 to lytic vacuoles. We find that changes in CLV2 activity have no detectabl...
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PMID: 21333538
PDF is available here.
Tian Li,
Shou-Ling Xu,
Juan A Oses-Prieto,
Sunita Putil,
Peng Xu,
Rui-Ju Wang,
Kathy H Li,
David A Maltby,
Liz-He An,
Alma L Burlingame,
Zhi-Ping Deng and
Zhi-Yong Wang
Abstract
We use two-dimensional difference gel electrophoresis (2-D DIGE) analysis of sub-cellular fractions of Arabidopsis thaliana proteome coupled with spot identification by tandem mass spectrometry to identify early cold-responsive proteins in Arabidopsis. These proteins include four enzymes involved in...
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PMID: 21242321
PDF is available here.
Abstract
We report the ability of Toc90 to support the import of Toc159 client proteins. We show that the overexpression of Toc90 partially complements the albino knockout of Toc159 and restores photoautotrophic growth. Several lines of evidence including proteome profiling demonstrate the import and accumul...
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PMID: 21220583
PDF is available here.
Abstract
We show that RIN4 phosphorylation activates RPM1. RIN4(142-176) is necessary and, with appropriate localization sequences, sufficient to support effector-triggered RPM1 activation, with the threonine residue at position 166 being critical. Phosphomimic substitutions at T166 cause effector-independen...
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PMID: 21320695
PDF is available here.
Abstract
The growing importance of vesicular trafficking and cytoskeleton dynamic reorganization during plant development requires the exploitation of novel experimental approaches. Several genetic and cell biological studies have used diverse pharmaceutical drugs that inhibit vesicular traff...
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PMID: 21090759
PDF is available here.
Abstract
We determined its crystal structure. AtGPA1 is structurally similar to animal G protein α subunits, but our crystallographic and biophysical studies revealed that it had distinct properties. Notably, the helical domain of AtGPA1 displayed pronounced intrinsic disorder and a tendency to disengage fr...
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PMID: 21304159
PDF is available here.
Abstract
We demonstrate that five NPY genes play a key role in Arabidopsis root gravitropism. NPY genes were previously identified as regulators of auxin-mediated organogenesis in a genetic pathway with the AGC kinases PID, PID2, WAG1, and WAG2. We show that all five NPY genes are highly expressed in primary...
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PMID: 20833732
PDF is available here.
Abstract
We demonstrate a physical association of RTE1 and ETR1 using in vivo and in vitro methods. Interaction of RTE1 and ETR1 was revealed in vivo by bimolecular fluorescence complementation (BiFC) in a tobacco cell transient assay and in stably transformed Arabidopsis. The association was also observed u...
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PMID: 20952388
PDF is available here.
Li-Qing Chen,
Bi-Huei Hou,
Sylvie Lalonde,
Hitomi Takanaga,
Mara L Hartung,
Xiao-Qing Qu,
Woei-Jiun Guo,
Jung-Gun Kim,
William Underwood,
Bhavna Chaudhuri,
Diane Chermak,
Ginny Antony,
Frank F White,
Shauna C Somerville,
Mary Beth Mudgett and
Wolf B Frommer
Abstract
We identified a new class of sugar transporters, named SWEETs, and show that at least six out of seventeen Arabidopsis, two out of over twenty rice and two out of seven homologues in Caenorhabditis elegans, and the single copy human protein, mediate glucose transport. Arabidopsis SWEET8 is essential...
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PMID: 21107422
PDF is available here.
Abstract
We described improved versions of MultiRound Gateway vectors. Here, we engineered a calcineurin B-like (CBL) pathway for potassium (K+) nutrition to demonstrate their effectiveness. Using the two improved entry vectors pL12R34H-Ap and pL34R12-Cm, and through 2-4 rounds of Gateway recombination react...
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PMID: 20708297
PDF is available here.
Abstract
We report on the identification of the ABA-induced transcriptional repressor Arabidopsis zinc-finger protein 2 (AZF2) as ABA signaling component. We isolated azf2-1 mutants lacking AZF2 full-length transcripts that were hypersensitive to ABA during seed germination. In line with a function of AZF2 i...
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PMID: 20619483
PDF is available here.
Abstract
We report a transporter, Nrat1 (Nramp aluminum transporter 1), specific for trivalent Al ion in rice. Nrat1 belongs to the Nramp (natural resistance-associated macrophage protein) family, but shares a low similarity with other Nramp members. When expressed in yeast, Nrat1 transports trivalent Al ion...
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PMID: 20937890
PDF is available here.
Abstract
We have previously shown that more than two thirds of Arabidopsis genes contain histone H3 methylation at lysine 4 (H3K4me) and that trimethylation of H3K4 (H3K4me3) is preferentially located at actively transcribed genes. In addition, several Arabidopsis mutants with locus-specific loss of H3K4me h...
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PMID: 20937886
PDF is available here.
Abstract
We show in Arabidopsis that the exclusion depends on a histone demethylase gene, IBM1 (increase in BONSAI methylation). Loss-of-function ibm1 mutation results in ectopic H3K9me and non-CG methylation in thousands of genes. The ibm1-induced genic H3K9me depends on both histone methylase KYP/SUVH4 and...
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PMID: 20834229
PDF is available here.
Abstract
We show that in virus-infected plants the enhanced expression of AGO1 mRNA is not accompanied by increased AGO1 protein accumulation. We also show that the induction of AGO1 mRNA level is a part of the host defence reaction, whereas the induction of miR168, which overlaps spatially with virus-occupi...
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PMID: 20823831
PDF is available here.
Abstract
We recently performed a genome-wide survey for endogenous targets silenced by MOM1 and identified loci that are concentrated around centromeres and rich in sequences homologous to the 24-nt small interfering RNAs (siRNAs) that accumulate in wild type plants. Further and independent analyses indicate...
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PMID: 21464619
PDF is available here.
Abstract
The extent to which vacuolar sugar transport activity affects molecular, cellular, and developmental processes in Arabidopsis (Arabidopsis thaliana) is unknown. Electrophysiological analysis revealed that overexpression of the tonoplast monosaccharide transporter TMT1 in a tmt1-2::tDNA mutant led to...
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PMID: 20709831
PDF is available here.
Abstract
We investigated 37 ethylene-inducible Arabidopsis transcription factor genes for their effects on the activation of ethylene signalling and insect defence. Twenty-eight of the 37 genes responded to both ethylene and HrpN Ea, and showed either increased or inhibited transcription, while 18 genes show...
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PMID: 20826953
PDF is available here.
Abstract
We studied germination in ethylene-related mutants of Arabidopsis. Mutations in the genes etr1 and ein2, which reduce ethylene responses, showed increased dormancy and a delay in germination in comparison with wild type. Mutations in etr1, ein2 and ein6 also resulted in increased sensitivity to ABA...
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PMID: 20826954
PDF is available here.
Abstract
We generated a whole array of 35S-OsWRKY transgenic Arabidopsis. In this study, we report that 35S-OsWRKY72 transgenic Arabidopsis, whose seed germination was retarded under normal conditions, emerged more sensitive to mannitol, NaCl, ABA stresses and sugar starvation than vector plants. Meanwhile,...
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PMID: 20826955
PDF is available here.
Abstract
We provide evidence that AHL20, an AT-hook containing DNA-binding protein, negatively regulates PTI. Overexpression of AHL20 as a stable transgene suppressed PAMP-induced NHO1 and FRK1 expression in Arabidopsis plants. Similarly, transient expression of the closely related family members AHL19, AHL1...
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PMID: 20738724
PDF is available here.
Abstract
We have proposed a possibility of another important sphingolipid function associated with cold acclimation. In animal cells, it has been known that the plasma membrane contains microdomains due to the chanracteristics of sphingolipids and sterols, and the sphingolipid- and sterol-enriched microdomai...
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PMID: 20818179
PDF is available here.
Abstract
We tested the hypothesis by replacing these cysteines with alanines and showed that depletions of one or both of the cysteine pairs do not hamper the function of CLV2 in SAM maintenance. In vitro peptide assay also showed that removal of the cysteine pairs did not affect the perception of CLV3 pepti...
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PMID: 20738721
PDF is available here.
Abstract
We found that plant-derived cytokinins promote resistance of Arabidopsis to Pseudomonas syringae pv. tomato DC3000 (Pst). Modulated cytokinin levels or signaling activity in CKX- or IPT-overexpressing plants or in ahk2 ahk3 mutants correlated with altered resistance. In fact, the cytokinin-activated...
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PMID: 20708590
PDF is available here.
Abstract
Of the known epigenetic control regulators found in plants, the Morpheus' molecule 1 (MOM1) protein is atypical in that the deletion of MOM1 does not affect the level of epigenetic marks controlling the transcriptional status of the genome. A short 197-amino-acid fragment of the MOM1 protein sequenc...
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PMID: 20693667
PDF is available here.
Abstract
Tubulin-folding cofactor A (TFC A) is a molecular post-chaperonin that is involved in the beta-tubulin-folding pathway. It has been identified in many organisms including yeasts, humans and plants. In this work, Arabidopsis thaliana TFC A was expressed in Escherichia coli and purified to homogeneity...
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PMID: 20693679
PDF is available here.
Abstract
We have now determined the structure of an Hsp90-CS-CHORD ternary complex, providing a framework for understanding the dynamic nature of Hsp90-Rar1-Sgt1 complexes. Mutational and biochemical analyses define the architecture of the ternary complex that recruits nucleotide-binding leucine-rich repeat...
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PMID: 20670895
PDF is available here.
Abstract
RNA interference pathways can involve amplification of secondary siRNAs by RNA-dependent RNA polymerases. In plants, RDR6-dependent secondary siRNAs arise from transcripts targeted by some microRNAs (miRNAs). Here, Arabidopsis thaliana secondary siRNAs from mRNA as well as trans-acti...
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PMID: 20562854
PDF is available here.
Abstract
We attempted to generate Arabidopsis transgenic lines that overexpress AtMYB30 under the control of the constitutive 35S promoter. However, no transgenic lines overexpressing AtMYB30 could be obtained, suggesting the existence of a molecular mechanism that negatively regulates AtMYB30 expression in...
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PMID: 20605724
PDF is available here.
Abstract
We describe two novel ddb1b T-DNA insertion mutants that are not embryo lethal, which we utilized as genetic tools to dissect DDB1b from DDB1a function. Information generated by these studies showed that the C-terminal part of the DDB1 proteins is critical for specific protein-protein interactions....
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PMID: 20499085
PDF is available here.
Abstract
We provide evidence for a function of the two-component signaling system in cold stress response in Arabidopsis. Cold significantly induced the expression of a subset of A-type ARR genes and of GUS in Pro(ARR7):GUS transgenic Arabidopsis. AHK2 and AHK3 were found to be primarily involved in mediatin...
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PMID: 20463025
PDF is available here.
Abstract
Our data suggest that differential regulation of TAS1 tasiRNA targets is involved in temperature-controlled gene expression....
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PMID: 20622450
PDF is available here.
Abstract
Genomic imprinting refers to the phenomenon that the expression of a gene copy depends on its parent of origin. The Arabidopsis imprinted FIS (Fertilisation-independent seed) genes, mea, fis2, and fie, play essential roles in the repression of central cell and the regulation of early endosperm devel...
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PMID: 20650847
PDF is available here.
Abstract
We describe a new protocol for immunolabelling meiotic proteins that allows the detection of a large range of proteins on strongly spread chromosomes throughout the entire meiotic process. We used this method to immunodetect MLH1, a crucial component of the meiotic recombination machinery, and found...
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PMID: 20628250
PDF is available here.
Abstract
We used the Arabidopsis seed coat to study the participation of the exocyst complex in polarized pectin delivery. • We characterized the amount of pectinaceous mucilage and seed coat structure in sec8 and exo70A1 exocyst mutants. Using a yeast two-hybrid screen, we identified a new interactor of t...
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PMID: 20618910
PDF is available here.
Abstract
We show, using cell-type-specific transcriptional effects of SHR and SCR combined with data from chromatin immunoprecipitation-based microarray experiments, that SHR regulates the spatiotemporal activation of specific genes involved in cell division. Coincident with the onset of a specific formative...
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PMID: 20596025
PDF is available here.
Abstract
We therefore renamed these two alleles lsd1-3 and lsd1-4, respectively. These two mutants exhibited an extensive cell death phenotype under cold stress conditions. Consistently, lsd1-3 plants exposed to cold showed up-regulation of the PR1 and PR2 genes, and increased accumulation of salicylic acid....
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PMID: 20456049
PDF is available here.
Abstract
We therefore renamed these two alleles lsd1-3 and lsd1-4, respectively. These two mutants exhibited an extensive cell death phenotype under cold stress conditions. Consistently, lsd1-3 plants exposed to cold showed up-regulation of the PR1 and PR2 genes, and increased accumulation of salicylic acid....
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PMID: 20456049
PDF is available here.
Abstract
We therefore renamed these two alleles lsd1-3 and lsd1-4, respectively. These two mutants exhibited an extensive cell death phenotype under cold stress conditions. Consistently, lsd1-3 plants exposed to cold showed up-regulation of the PR1 and PR2 genes, and increased accumulation of salicylic acid....
|
PMID: 20456049
PDF is available here.
Abstract
We therefore renamed these two alleles lsd1-3 and lsd1-4, respectively. These two mutants exhibited an extensive cell death phenotype under cold stress conditions. Consistently, lsd1-3 plants exposed to cold showed up-regulation of the PR1 and PR2 genes, and increased accumulation of salicylic acid....
|
PMID: 20456049
PDF is available here.
Abstract
We therefore renamed these two alleles lsd1-3 and lsd1-4, respectively. These two mutants exhibited an extensive cell death phenotype under cold stress conditions. Consistently, lsd1-3 plants exposed to cold showed up-regulation of the PR1 and PR2 genes, and increased accumulation of salicylic acid....
|
PMID: 20456049
PDF is available here.
Abstract
We therefore renamed these two alleles lsd1-3 and lsd1-4, respectively. These two mutants exhibited an extensive cell death phenotype under cold stress conditions. Consistently, lsd1-3 plants exposed to cold showed up-regulation of the PR1 and PR2 genes, and increased accumulation of salicylic acid....
|
PMID: 20456049
PDF is available here.
Abstract
We therefore renamed these two alleles lsd1-3 and lsd1-4, respectively. These two mutants exhibited an extensive cell death phenotype under cold stress conditions. Consistently, lsd1-3 plants exposed to cold showed up-regulation of the PR1 and PR2 genes, and increased accumulation of salicylic acid....
|
PMID: 20456049
PDF is available here.
Abstract
We therefore renamed these two alleles lsd1-3 and lsd1-4, respectively. These two mutants exhibited an extensive cell death phenotype under cold stress conditions. Consistently, lsd1-3 plants exposed to cold showed up-regulation of the PR1 and PR2 genes, and increased accumulation of salicylic acid....
|
PMID: 20456049
PDF is available here.
Abstract
We therefore renamed these two alleles lsd1-3 and lsd1-4, respectively. These two mutants exhibited an extensive cell death phenotype under cold stress conditions. Consistently, lsd1-3 plants exposed to cold showed up-regulation of the PR1 and PR2 genes, and increased accumulation of salicylic acid....
|
PMID: 20456049
PDF is available here.
Abstract
We therefore renamed these two alleles lsd1-3 and lsd1-4, respectively. These two mutants exhibited an extensive cell death phenotype under cold stress conditions. Consistently, lsd1-3 plants exposed to cold showed up-regulation of the PR1 and PR2 genes, and increased accumulation of salicylic acid....
|
PMID: 20456049
PDF is available here.
Abstract
We therefore renamed these two alleles lsd1-3 and lsd1-4, respectively. These two mutants exhibited an extensive cell death phenotype under cold stress conditions. Consistently, lsd1-3 plants exposed to cold showed up-regulation of the PR1 and PR2 genes, and increased accumulation of salicylic acid....
|
PMID: 20456049
PDF is available here.
Abstract
We therefore renamed these two alleles lsd1-3 and lsd1-4, respectively. These two mutants exhibited an extensive cell death phenotype under cold stress conditions. Consistently, lsd1-3 plants exposed to cold showed up-regulation of the PR1 and PR2 genes, and increased accumulation of salicylic acid....
|
PMID: 20456049
PDF is available here.
Abstract
We therefore renamed these two alleles lsd1-3 and lsd1-4, respectively. These two mutants exhibited an extensive cell death phenotype under cold stress conditions. Consistently, lsd1-3 plants exposed to cold showed up-regulation of the PR1 and PR2 genes, and increased accumulation of salicylic acid....
|
PMID: 20456049
PDF is available here.
Abstract
We therefore renamed these two alleles lsd1-3 and lsd1-4, respectively. These two mutants exhibited an extensive cell death phenotype under cold stress conditions. Consistently, lsd1-3 plants exposed to cold showed up-regulation of the PR1 and PR2 genes, and increased accumulation of salicylic acid....
|
PMID: 20456049
PDF is available here.
Abstract
We therefore renamed these two alleles lsd1-3 and lsd1-4, respectively. These two mutants exhibited an extensive cell death phenotype under cold stress conditions. Consistently, lsd1-3 plants exposed to cold showed up-regulation of the PR1 and PR2 genes, and increased accumulation of salicylic acid....
|
PMID: 20456049
PDF is available here.
Abstract
We therefore renamed these two alleles lsd1-3 and lsd1-4, respectively. These two mutants exhibited an extensive cell death phenotype under cold stress conditions. Consistently, lsd1-3 plants exposed to cold showed up-regulation of the PR1 and PR2 genes, and increased accumulation of salicylic acid....
|
PMID: 20456049
PDF is available here.