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Leghemoglobin (0):
Recent article
Leghemoglobin
Plant Physiology (152)3 2010
Abstract
The role of nitrogen metabolism in the survival of prolonged periods of waterlogging was investigated in highly flood-tolerant, nodulated Lotus japonicus plants. Alanine production revealed to be a critical hypoxic pathway. Alanine is the only amino acid whose biosynthesis is not inhibited by nitrog...
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PMID: 20089769
PDF is available here.
PDF is available here.
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Abstract
A nonenzymatic glycation of the recombinant leghemoglobin expressed in Escherichia coli cells was demonstrated for the first time. This process involved the heme pocket and gave low-spin leghemoglobin species. A correlation between the degree of E. coli protein glycation and synthesis of poly-beta-h...
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PMID: 20586285
PDF is available here.
PDF is available here.
Abstract
We used a leghemoglobin-RNA-interference (LbRNAi) line of Lotus japonicus, which is devoid of leghemoglobins and has elevated levels of free-oxygen in its nodules. Bacteroids in LbRNAi nodules showed altered ultrastructure indicating changes in bacterial differentiation. Transcript analysis of 189 p...
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PMID: 19522562
PDF is available here.
PDF is available here.
Abstract
We present a novel approach called SNAP for non-sequential pair-wise structural alignment. Starting from an initial alignment, our approach iterates over a two-step process consisting of a superposition step and an alignment step, until convergence. We propose a novel greedy algorithm to construct b...
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PMID: 19507290
PDF is available here.
PDF is available here.
Abstract
We studied the effects of cadmium, chromium and copper used both separately and as mixtures, on over all growth of pea plants inoculated with Rhizobium sp. Among the metals, copper was most toxic for pea plants and decreased the seed yield by 15% at 1,338 mg kg(-1) compared to control plants whereas...
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PMID: 18368281
PDF is available here.
PDF is available here.
Abstract
We have cloned and characterized a Hb (ppHb) from the root nodules of the ancient caesalpinoid legume Chamaecrista fasciculata. Protein sequence, modeling data, and spectral analysis indicated that the properties of ppHb are intermediate between that of nsHb and Lb, suggesting that ppHb resembles a...
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PMID: 18214970
PDF is available here.
PDF is available here.
Journal of Molecular Biology (378)3 2008
Abstract
Our high-precision kinetic analysis also reveals an additional slow phase during the folding of apoleghemoglobin, which is not observed for apomyoglobin. Hydrogen exchange pulse-labeling experiments show that the slow-folding phase is associated with residues in the CE loop, which probably forms non...
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PMID: 18384808
PDF is available here.
PDF is available here.
Environmental Pollution (151)1 2008
Abstract
In the present experiment the seeds of Cicer arietinum (L.) cv. Uday were inoculated with specific Rhizobium grown in sandy loam soil and were allowed to grow for 15 days. At this stage, the seedlings were supplied with 0, 50, 100 or 150 microM of cadmium in the form of cadmium chloride and sprayed...
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PMID: 17481788
PDF is available here.
PDF is available here.
Abstract
We found that loss of leghemoglobin results in significantly lower H(2)O(2) levels in nodules. Transcript levels and catalytic activities of ascorbate-glutathione cycle enzymes involved in H(2)O(2) detoxification as well as concentrations of reduced ascorbate were also altered in LbRNAi nodules. Thu...
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PMID: 17990967
PDF is available here.
PDF is available here.
Chemosphere (70)1 2007
Abstract
The nickel and zinc tolerant plant growth promoting Bradyrhizobium sp. (vigna) RM8 was isolated from nodules of greengram, grown in metal contaminated Indian soils. The plant growth promoting (PGP) potentials of strain RM8 was assessed both in the presence and absence of nickel and zinc under in vit...
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PMID: 17723236
PDF is available here.
PDF is available here.
Abstract
We enquire how the functional parameters of the process are optimized in particular organisms. The ligand-binding properties of the proteins, myoglobin and plant symbiotic hemoglobins, we discover, suggest that they have been adapted under genetic selection pressure for optimal performance. Since ca...
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PMID: 17573206
PDF is available here.
PDF is available here.
Abstract
This review describes contributions to the study of plant hemoglobins (Hbs) from a historical perspective with emphasis on non-symbiotic Hbs (nsHbs). Plant Hbs were first identified in soybean root nodules, are known as leghemoglobins (Lbs) and have been characterized in detail. It is widely accepte...
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PMID: 17540516
PDF is available here.
PDF is available here.
Journal of Molecular Biology (371)1 2007
Abstract
We report the crystal structure of the cyanide-bound hexacoordinate hemoglobin from barley. This presents the first opportunity to examine conformational changes in plant hexacoordinate hemoglobins upon exogenous ligand binding, and reveals structural mechanisms for stabilizing the high-energy penta...
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PMID: 17560601
PDF is available here.
PDF is available here.
Abstract
We analyzed by means of a combination of classical Molecular Dynamics (MD) and Hybrid Quantum-Classical (QM/MM) techniques the existence of multiple conformations in the distal site of heme proteins and their influence on oxygen affinity regulation. We considered two representative examples: soybean...
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PMID: 17469189
PDF is available here.
PDF is available here.
Bioresource Technology (98)5 2007
Abstract
The effect of composted textile sludge on growth, nodulation and nitrogen fixation of soybean and cowpea was evaluated in a greenhouse experiment. The compost was incorporated into soil at 0, 9.5, 19 and 38 t ha(-1) (bases upon the N requirement of the crops, i.e., 0, 50, 100 and 200 kg available N...
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PMID: 16782329
PDF is available here.
PDF is available here.
Journal of Bacteriology (189)5 2007
Abstract
We propose that C. jejuni CydAB be renamed CioAB (cyanide-insensitive oxidase), as in Pseudomonas aeruginosa. We measured the oxygen affinity of each oxidase, using a highly sensitive assay that exploits globin deoxygenation during respiration-catalyzed oxygen uptake. The CioAB-type oxidase exhibite...
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PMID: 17172349
PDF is available here.
PDF is available here.
Microbiology (153)Pt 2 2007
Abstract
It is becoming recognized that leghaemoglobin constitutes an important buffer for the cytotoxic nitric oxide radical (NO(*)) in root nodules, although the sources of this NO(*) within nodules are unclear. In Bradyrhizobium japonicum bacteroids, NO(*) can be produced through the denitrification proce...
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PMID: 17259612
PDF is available here.
PDF is available here.
Abstract
Bacterial nodulation factors (NFs) are essential signaling molecules for the initiation of a nitrogen-fixing symbiosis in legumes. NFs are perceived by the plant and trigger both local and distant responses, such as curling of root hairs and cortical cell divisions. In addition to their requirement...
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PMID: 17313164
PDF is available here.
PDF is available here.
Abstract
We present an investigation of the proximal regulation of oxygen affinity in Fe(II) histidine coordinated heme proteins by means of computer simulation. Density functional theory calculations on heme model systems are used to analyze three proximal effects: charge donation, rotational position, and...
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PMID: 16984195
PDF is available here.
PDF is available here.
Abstract
We assessed bioavailability of iron from LHb (either partially purified (LHbA) or purified (LHbD)) with and without food matrix and compared it with that from bovine hemoglobin (BHb), ferrous sulfate (FeSO4), or SRN. Bioavailability of each treatment was normalized to 100% of the FeSO4 treatment. Wh...
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PMID: 16478282
PDF is available here.
PDF is available here.
Abstract
These results indicate a likely involvement of ROS in the effects of environmental stresses on BNF. Furthermore, these results support the occurrence of two regulation pathways for BNF under oxidative stress, one of these involving carbon shortage and the other involving Lb/oxygen flux....
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PMID: 16415332
PDF is available here.
PDF is available here.
Predicting alternate structure attainment and amyloidogenesis: a nonlinear signal analysis approach.
Abstract
We show that such an analysis gives us an easy handle in determining sequences within the proteins that may be important in beta-sheet formation leading to amyloidosis....
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PMID: 16263079
PDF is available here.
PDF is available here.
Abstract
We have shown that different forms of Lb react not only with NO. but also with so-called reactive nitrogen species derived from it, among others peroxynitrite and nitrite. Because of the wider active-site pocket, the rate constants measured in this work for NO. and for nitrite binding to metLb are 1...
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PMID: 16267660
PDF is available here.
PDF is available here.
Abstract
We have determined the second-order rate constants of the reactions of soybean oxyleghemoglobin with nitrogen monoxide and peroxynitrite. At pH 7.3 and 20 degrees C, the values are on the order of 10(8) and 10(4) M-1 s-1, respectively. In the presence of physiological amounts of CO2 (1.2 mM), the se...
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PMID: 16267661
PDF is available here.
PDF is available here.
Current Biology (15)6 2005
Abstract
How do plants create an environment in which symbiotic bacteria can reduce enough N2 to provide the plant with sufficient ammonium for growth? Gene silencing has now been used to show that legume haemoglobins are crucial.
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PMID: 15797009
PDF is available here.
PDF is available here.
Current Biology (15)6 2005
Abstract
We abolished symbiotic leghemoglobin synthesis in nodules of the model legume Lotus japonicus. This caused an increase in nodule free oxygen, a decrease in the ATP/ADP ratio, loss of bacterial nitrogenase protein, and absence of SNF. However, LbRNAi plants grew normally when fertilized with mineral...
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PMID: 15797021
PDF is available here.
PDF is available here.
Journal of Experimental Botany (56)413 2005
Abstract
In this study the further characterization of the Vicia faba leghaemoglobin promoter pVfLb29 is presented that was previously shown to be specifically active in the infected cells of root nodules and in arbuscule-containing cells of mycorrhizal roots. Using promoter studies in transgenic hairy roots...
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PMID: 15668224
PDF is available here.
PDF is available here.
Abstract
The symbiotic phenotype of five Tn5-induced mutants of Rhizobium etli affected in different anabolic pathways (namely, gluconeogenesis and biosynthesis of lysine, purine, or pyrimidine) was analyzed. These mutants induced, on the root of Phaseolus vulgaris, a normal early sequence of morphogenetics...
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PMID: 15497397
PDF is available here.
PDF is available here.
Biochemistry (Washington) (43)20 2004
Abstract
Detailed comparisons of the carbon monoxide FTIR spectra and ligand-binding properties of a library of E7, E11, and B10 mutants indicate significant differences in the role of electrostatic interactions in the distal pockets of wild-type sperm whale myoglobin and soybean leghemoglobin. In myoglobin,...
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PMID: 15147208
PDF is available here.
PDF is available here.
Journal of Plant Physiology (161)5 2004
Abstract
Endogenous free polyamines, nitrogenase (EC 1.1.8.6.1, acetylene reduction), and leghaemoglobin (pyridine-hemochrome assay) levels were compared among five genotypes of developing Vigna root nodules grown under field conditions. Nitrogenase activity and leghaemoglobin level attained a peak at the fl...
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PMID: 15202713
PDF is available here.
PDF is available here.
Plant Physiology (134)2 2004
Abstract
Mathematical models were developed to test the likelihood that large cytosolic adenylate concentration gradients exist across the bacteria-infected cells of legume nodules. Previous studies hypothesized that this may be the case to account for the unusually low adenylate energy charge (AEC; 0.65) me...
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PMID: 14739350
PDF is available here.
PDF is available here.
Journal of Experimental Botany (55)394 2004
Abstract
These results correlate with the expression of the leghaemoglobin gene, which encodes the major haem-containing protein present in the nodule. Contrary to its animal counterpart, alfalfa HO1 was not induced by pro- oxidant compounds including H(2)O(2), paraquat and sodium nitroprusside, suggesting t...
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PMID: 14623903
PDF is available here.
PDF is available here.
Abstract
The VfLb29 leghemoglobin gene promoter was polymerase chain reaction-amplified from a Vicia faba genomic library and was fused to the gusAint coding region. Expression of the chimeric gene was analyzed in transgenic hairy roots of the legumes V. faba, V. hirsuta, and Medicago truncatula as well as i...
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PMID: 14714869
PDF is available here.
PDF is available here.
Biophysical Journal (85)5 2003
Abstract
Reduced lattice models of proteins and Monte Carlo dynamics were used to simulate the initial stages of the unfolding of several proteins of various structural types, and the results were compared to experiment. The models semiquantitatively reproduce the approximate order of events of unfolding as...
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PMID: 14581227
PDF is available here.
PDF is available here.
Abstract
We have exploited the intrinsic conformational flexibility of leghemoglobin to reengineer the heme active site architecture of the molecule by replacement of the mobile His61 residue with tyrosine (H61Y variant). The electronic absorption spectrum of the ferric derivative of H61Y is similar to that...
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PMID: 14522591
PDF is available here.
PDF is available here.
Abstract
New developments have forced a re-evaluation of our understanding of the structure and function of hemoglobins. Leghemoglobins regulate oxygen affinity through a mechanism different from that of myoglobin using a novel combination of heme pocket amino acids that lower the oxygen affinity. The hexaco...
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PMID: 12927972
PDF is available here.
PDF is available here.
Plant Physiology (131)3 2003
Abstract
We examined a novel symbiotic mutant, crinkle (Ljsym79), from the model legume Lotus japonicus. On nitrogen-starved medium, crinkle mutants inoculated with the symbiont bacterium Mesorhizobium loti MAFF 303099 showed severe nitrogen deficiency symptoms. This mutant was characterized by the productio...
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PMID: 12644658
PDF is available here.
PDF is available here.
Journal of Experimental Botany (54)384 2003
Abstract
These results contrast with a previous report, apparently based on limited data, but they are consistent with anatomical and tracer studies showing higher gas permeability near the tip....
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PMID: 12598578
PDF is available here.
PDF is available here.
Abstract
Leghemoglobins facilitate diffusion of oxygen through root tissue to a bacterial terminal oxidase in much the same way that myoglobin transports oxygen from blood to muscle cell mitochondria. Leghemoglobin serves an additional role as an oxygen scavenger to prevent inhibition of nitrogen fixation. F...
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PMID: 12486718
PDF is available here.
PDF is available here.
Acta Biologica Hungarica (54)1 2003
Abstract
The recent review summarizes the major achievements in discovery of role of phytoglobins in mediation of nitric oxide generated cellular functions in higher plants. Genes encoding non-symbiotic hemoglobins have been cloned from several plant species. The expression pattern of these genes show tissue...
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PMID: 12705318
PDF is available here.
PDF is available here.
Abstract
The effects of electrostatic substitutions on the spectroscopic, ligand binding, and redox properties of the heme in leghemoglobin have been examined by replacement of the proximal leucine 88 residue with an aspartic acid residue (Leu88Asp). Electronic and resonance Raman spectra of the ferric deriv...
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PMID: 11913977
PDF is available here.
PDF is available here.
Journal of Experimental Botany (53)368 2002
Abstract
Soybean (Glycine max L. Merr.) plants exposed to 10 mM KNO(3) for a 4 d period were used to test the correlation between nitrogenase activity, gene expression and sucrose metabolism. Nitrate caused the down-regulation of sucrose synthase (SS) transcripts within 1 d, although a decline in nodule SS a...
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PMID: 11847240
PDF is available here.
PDF is available here.
Abstract
These results are discussed in the context of current hypotheses for proximal heme pocket structure and function.
Copyright 2002 Wiley-Liss, Inc....
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PMID: 11835502
PDF is available here.
PDF is available here.
Abstract
We revisit two widely used families of threading potentials: the pairwise and profile models. To design optimal scoring functions we use linear programming (LP). The LP protocol makes it possible to measure the difficulty of a particular training set in conjunction with a specific form of the scorin...
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PMID: 11599028
PDF is available here.
PDF is available here.
Abstract
The differences in the tryptophan (Trp) fluorescence of native (control) Lb"a" and experimental substance isolated from nodules of the Williams' soy beans variety treated with trifluraline at a concentration of 2.1 x 10(-10) M have been studied. A positively charged environment has been proved for t...
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PMID: 11767847
PDF is available here.
PDF is available here.
Plant Physiology (125)2 2001
Abstract
Five distinct cDNA clones for leghemoglobin (Lb) were isolated from pea (Pisum sativum) nodules. They were classified into two groups designated PsLbA and PsLbB according to sequence homology, O(2)-binding affinities of the recombinant proteins, and in situ localization of the mRNAs. The PsLbB group...
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PMID: 11161022
PDF is available here.
PDF is available here.
Journal of Experimental Botany (52)355 2001
Abstract
These results do not support the hypothesis that ABA directly regulates SS activity. However, they do suggest the occurrence of at least two different control pathways in nodules under environmental constraints, which include ABA being involved in a Lb/oxygen-related control of nitrogen fixation....
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PMID: 11283173
PDF is available here.
PDF is available here.
Abstract
Mesorhizobium loti and Rhizobium etli are microsymbionts of the Lotus and Phaseolus spp., respectively, and secrete essentially the same Nod factors. Lotus japonicus efficiently formed root nodules with R. etli CE3, irrespective of the presence or absence of a flavonoid-independent transcription act...
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PMID: 11204780
PDF is available here.
PDF is available here.
Abstract
Method for simultaneous obtaining in homogeneous state of two main forms of metleghemoglobin reductase and main leghemoglobin components from lupine nodules was worked out. The method included steps of saturation with ammonium sulphate (40-80%), gel-filtration on Ultrogel AcA 44, isoelectric focusin...
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PMID: 11357430
PDF is available here.
PDF is available here.
Abstract
To test the hypothesis that the folding pathways of evolutionarily related proteins with similar three-dimensional structures but widely different sequences should be similar, the folding pathway of apoleghemoglobin has been characterized using stopped-flow circular dichroism, heteronuclear NMR pulse...
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PMID: 10932254
PDF is available here.
PDF is available here.
Abstract
We have identified a novel type of DNA-binding protein (CPP1) interacting with the promoter of the soybean leghemoglobin gene Gmlbc3. The DNA-binding domain of CPP1 contains two similar Cys-rich domains with 9 and 10 Cys, respectively. Genes encoding similar domains have been identified in Arabidops...
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PMID: 10859345
PDF is available here.
PDF is available here.
Abstract
A strategic assessment of the contributions of two active-site hydrogen bonds in the binding of nicotinate to recombinant ferric soybean leghaemoglobin a (rLb) was carried out by mutagenic replacement of the hydrogen-bonding residues (H61A and Y30A variants) and by complementary chemical substitutio...
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PMID: 10785378
PDF is available here.
PDF is available here.
Abstract
We have analysed its functional activity in transgenic Lotus corniculatus. A fusion construct between the lbI promoter and the GUS reporter gene was expressed mainly in the central zone of the root nodule, but the product was also detected in the non-nodule root zone and in roots in tissue culture....
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PMID: 10778735
PDF is available here.
PDF is available here.
Abstract
Systematic sequencing of expressed sequence tags (ESTs) can give a global picture of the assembly of genes involved in the development and function of organs. Indeterminate nodules representing different stages of the developmental program are especially suited to the study of organogenesis. With th...
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PMID: 10656586
PDF is available here.
PDF is available here.
Casuarina glauca prenodule cells display the same differentiation as the corresponding nodule cells.
Abstract
We examine the differentiation of actinorhizal prenodule cells in comparison to nodule cells with regard to both symbionts. Our findings indicate that prenodules represent primitive symbiotic organs whose cell types display the same characteristics as their nodule counterparts. The results are discu...
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PMID: 10656591
PDF is available here.
PDF is available here.
Abstract
An electrochemical cell for simultaneous potentiometric and spectrophotometric measurements of solutions of various substances is described. A schematic diagram of the cell, its advantages, method of use, and possible areas of application are discussed. The cell is suggested to be used for electroch...
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PMID: 10867958
PDF is available here.
PDF is available here.
Biopolymers (57)5 2000
Abstract
We report the visible and Soret absorption bands, down to cryogenic temperatures, of the ferrous nicotinate adducts of native and deuteroheme reconstituted horse heart myoglobin in comparison with soybean leghemoglobin-a. The band profile in the visible region is analyzed in terms of vibronic coupli...
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PMID: 10958321
PDF is available here.
PDF is available here.
Biochemistry (Washington) (38)50 1999
Abstract
We report the first characterization of ferric nitrosylleghemoglobin (Lb(III)NO) and XAS experiments performed on frozen aqueous solutions of Lb(II)NO and Lb(III)NO at 10 K. The XANES and electronic spectra of the NO adducts are similar in shape and energies to the myoglobin (Mb) analogues. The envi...
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PMID: 10600110
PDF is available here.
PDF is available here.
Phytochemistry (52)7 1999
Effectiveness of ascorbate and ascorbate peroxidase in promoting nitrogen fixation in model systems.
Abstract
These results support the conclusion that ascorbate and ascorbate peroxidase are beneficial for maintaining conditions favorable for N2 fixation in nodules....
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PMID: 10647213
PDF is available here.
PDF is available here.
Plant Molecular Biology (40)1 1999
Abstract
A cDNA containing a homeobox sequence was isolated from a soybean nodule-specific expression library. This homeobox cDNA, Ndx (nodulin homeobox), represents a small gene family with at least two members in soybean (Glycine max) and three in Lotus japonicus. One complete 3304 bp Ndx cDNA from L. japo...
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PMID: 10394946
PDF is available here.
PDF is available here.