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Phycoerythrin (0):
Recent article
Phycoerythrin
Abstract
We have evaluated in vitro cytotoxicity of cyanobacterial phycoerythrin (C-PE) on three human cell lines by cell proliferation and neutral red uptake assays. No toxic effects of C-PE were observed to any of the cell lines tested. The protective role of purified C-PE to potassium permanganate-mediate...
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PMID: 20564513
PDF is available here.
PDF is available here.
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Abstract
This study explored the chronic and harmful effects of different doses of the triazole fungicide, tebuconazole, on the growth, and metabolic and enzymatic variations in the filamentous paddy field cyanobacterium, Westiellopsis prolifica Janet. The growth of the cyanobacterium was determined by an es...
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PMID: 20668408
PDF is available here.
PDF is available here.
Avian Pathology (39)1 2010
Abstract
A flow cytometric method for the identification of chicken blood leukocyte subpopulations and thrombocytes was developed. An anti-chicken CD45 phycoerythrin-labelled antibody was used to separate leukocytes from red blood cell nuclei. Leukocytes and thrombocytes were identified using a combination o...
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PMID: 20390535
PDF is available here.
PDF is available here.
Abstract
We report the development of a new generation of green fiber lasers that can be engineered to emit in the range between 532 and 561 nm. A 550-nm green fiber laser was integrated into both a BD LSR II cuvette and FACSVantage DiVa jet-in-air cell sorter. This laser wavelength avoided both the fluoresc...
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PMID: 19777600
PDF is available here.
PDF is available here.
Abstract
Dynamic field gradient focusing (DFGF) utilizes an electric field gradient established by a computer-controlled electrode array to separate and concentrate charged analytes at unique axial positions. Traditionally, DFGF has been restricted to the analysis of negatively charged species due to limitat...
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PMID: 19722517
PDF is available here.
PDF is available here.
British Journal of Nutrition (102)1 2009
Abstract
In the present study, the protective role of purified C-phycoerythrin (C-PE) against diabetic complications and Cu-mediated lipoprotein oxidation was evaluated. C-PE (25 and 50 mg/kg body weight per d) was administered to experimental streptozotocin-nicotinamide-induced type 2 diabetic male rats for...
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PMID: 19123960
PDF is available here.
PDF is available here.
Abstract
These results suggest that pink pigment is a phycoerythrin-like protein. It inhibited the growth of green algae, Chlorella fusca and Chlamydomonas reinhardtii, but not other cyanobacteria or true bacteria. The concentration at which growth inhibition 50% occurred was 0.5, 6 and more than 10 mg ml(-1...
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PMID: 19271155
PDF is available here.
PDF is available here.
Archives of Toxicology (83)5 2009
Abstract
We analyzed the effects of the three macrolide antibiotics erythromycin, clarithromycin and azithromycin on human endothelial cells in vitro. As a positive control quinupristin/dalfopristin was studied. The cytotoxicity of all substances was analyzed by a modified MTT cytotoxicity assay with 3T3-fib...
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PMID: 19052724
PDF is available here.
PDF is available here.
Abstract
We isolated 28 Pseudanabaena strains from the Baltic Sea (BS) and the Albufera de Valencia (AV; Spain). By combining phenotypic and genotypic approaches, the phylogeny, diversity and evolutionary diversification of these isolates were explored. Analysis of the in vivo absorption spectra of the Pseud...
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PMID: 18769459
PDF is available here.
PDF is available here.
Abstract
The indicator function of the fluorescence signals of the cyanopigments phycocyanin and phycoerythrin as early warning parameters against the microcystins in drinking water was investigated by lab- and pilot-scale studies. The early warning function of the fluorescence signals was examined with rega...
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PMID: 19403966
PDF is available here.
PDF is available here.
Abstract
We start by presenting scaling issues and challenging limitations for miniaturized chemical nanosensors. Advantages of using nanoprobes e.g., isolated nanowires, as the components in chemical sensing are discussed. How the observation of the physical property can beneficially change with isomorphic...
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PMID: 19488700
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (283)41 2008
Abstract
We present the first crystal structures of a PEB biosynthetic enzyme. Structures of the substrate complex were solved at 1.8- and 2.1-A resolution and of the substrate-free form at 1.55-A resolution. The overall folding revealed an alpha/beta/alpha-sandwich with similarity to the structure of phycoc...
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PMID: 18662988
PDF is available here.
PDF is available here.
Abstract
We report on steady-state and time-resolved single-molecule fluorescence measurements performed on a phycobiliprotein, R-phycoerythrin (RPE), assembled on silver nanostructures. Single-molecule measurements clearly show that RPE molecules display a 10-fold increase in fluorescence intensity, with a...
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PMID: 18690697
PDF is available here.
PDF is available here.
Abstract
We have addressed the question of whether the toxic, bloom-forming dinoflagellate Dinophysis norvegica practice kleptoplasty or if they replicate their own plastid DNA. Dividing (G2) and non-dividing (G1) D. norvegica cells from a natural population were physically separated with a flow cytometer ba...
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PMID: 18518896
PDF is available here.
PDF is available here.
Abstract
We demonstrate the flow cytometric applications of silicon avalanche photodiodes (APDs), which have improved red sensitivity and a working fluorescence detection range beyond 1,000 nm. A comparison of the wavelength-dependent performance of the APD and PMT was carried out using pulsed light-emitting...
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PMID: 18612992
PDF is available here.
PDF is available here.
Bioresource Technology (99)10 2008
Abstract
Our study indicates N. lobatus to be a promising bioresource for enhanced production of nutritionally rich biomass, pigments and antioxidants. The study also suggests that P and Fe are potentially effective supplements for scale-up production for commercial application....
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PMID: 17919902
PDF is available here.
PDF is available here.
Toxicology (248)1 2008
Abstract
We have evaluated protective action of purified cyanobacterial phycoerythrin (C-PE) on carbon tetrachloride-induced hepatic and renal toxicity in male rats. Rats were orally treated with 25 and 50mg/kg BW of C-PE along with CCl(4) (50% CCl(4), 0.5 ml/kg BW, intraperitoneally) for 28 consecutive days...
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PMID: 18440118
PDF is available here.
PDF is available here.
FEMS Microbiology Letters (283)2 2008
Abstract
Phycoerythrin-containing Synechococcus strains are unicellular cyanobacteria that are of great ecological importance in the marine environment. These organisms are known to be susceptible to infection by cyanophages (viruses that infect cyanobacteria). The infection cycle takes several hours and dur...
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PMID: 18479290
PDF is available here.
PDF is available here.
Abstract
We thought about a B-cell proliferation carrying a surface receptor recognizing PE: these B-cells were directly labeled with streptavidin-PE, indeed. Moreover, the immunodots from the patient were able to fix the streptavidin-PE. Finally, this unusual immunophenotyping was solved by using antibodies...
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PMID: 18061954
PDF is available here.
PDF is available here.
Abstract
CD34 positive stem/progenitor cells have a smaller volume and diameter than CD45 positive mononuclear cells in HPC-A samples. In the present study the CD34 stem/progenitor cells had a considerably larger diameter than that of stem cells previously reported in the literature. With the availability of...
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PMID: 18383271
PDF is available here.
PDF is available here.
Abstract
Our findings suggest that the Synechococcus heterotrophy plays an important role in cell's metabolism, and that the photoheterotrophic behavior, together with their chromatic adaptation capability, might represent the key for the absolute dominance of this genus in the Adriatic Sea....
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PMID: 18336557
PDF is available here.
PDF is available here.
Current Biology (18)6 2008
Abstract
We investigate four such genes in two cyanophages that both infect abundant Prochlorococcus strains [8]: homologs of heme oxygenase (ho1), 15,16-dihydrobiliverdin:ferredoxin oxidoreductase (pebA), ferredoxin (petF) in the myovirus P-SSM2, and a phycocyanobilin:ferredoxin oxidoreductase (pcyA) homolo...
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PMID: 18356052
PDF is available here.
PDF is available here.
Acta Histochemica (110)3 2008
Abstract
TO-PRO-3 iodide (TP3), a monomeric cyanine nucleic acid stain with a peak absorbance at 642 nm and emission at 661 nm, is best excited by a helium-neon (HeNe) laser (633nm). It was tested in monocytes and different cell lines under conditions of different fixatives, dye concentrations, labeling kine...
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PMID: 18160099
PDF is available here.
PDF is available here.
Abstract
Fluorescence correlation spectroscopy (FCS) utilizes temporal fluctuations in fluorescence emission to extract quantitative measures of inter- or intramolecular dynamics or molecular motions of probe molecules, which occur on submicrosecond to second timescales. In typical experiments, one can readi...
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PMID: 17964946
PDF is available here.
PDF is available here.
Thrombosis Research (122)5 2008
Abstract
Diversity in the staining intensity of isotype controls is a potential source of error in the characterisation and quantification of MPs by flow cytometry. The use of antigen negative MPs to adjust instrument settings is suggested....
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PMID: 18304614
PDF is available here.
PDF is available here.
Abstract
We show that coexistence of red and green picocyanobacteria in the Baltic Sea is widespread. The diversity and phylogeny of red and green picocyanobacteria was analysed using three different genes: 16S rRNA-ITS, the cpeBA operon of the red PE pigment and the cpcBA operon of the green PC pigment. Seq...
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PMID: 17903216
PDF is available here.
PDF is available here.
Abstract
Analytical methods characterizing the immunogenicity of antigens are useful for monitoring, characterizing and predicting antibody responses to therapeutic biologics or vaccines. Distinct Luminex microspheres coupled with protein G, anti-human immunoglobulin (Ig)A, anti-human IgM and anti-human IgE...
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PMID: 18287660
PDF is available here.
PDF is available here.
New Phytologist (179)3 2008
Abstract
I (PSI) components remained constant. Total levels of phycobiliproteins remained unchanged according to single-cell in vivo spectra. However, the regular 16-kDa phycoerythrin band decreased and finally disappeared 16-20 d after initiation of Fe limitation, concomitant with the accumulation of a 20-k...
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PMID: 18513224
PDF is available here.
PDF is available here.
Abstract
The mean ABC value for T-lymphocytes prepared from fresh whole blood using CD4-PE conjugate (48,321) was consistent with previous results, and it was much higher than the mean ABC using CD4-FITC Mab (22,156). The mean ABC value for CYTO-TROL cells using CD4-PE conjugate (43,090) was also higher than...
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PMID: 17474131
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (282)41 2007
Abstract
We identified two gene families encoding phycoerythrin alpha and light-harvesting complex proteins from an expressed sequence tag library of the cryptophyte Guillardia theta. The proteins bear a bipartite topogenic signal responsible for the transport of nuclear encoded proteins via the ER into the...
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PMID: 17702756
PDF is available here.
PDF is available here.
Glycobiology (17)10 2007
Abstract
VIP36-like protein (VIPL) was identified as an endoplasmic reticulum (ER) resident protein with homology to VIP36, a cargo receptor involved in the transport of glycoproteins within cells. Although VIPL is structurally similar to VIP36, VIPL is thought not to be a lectin, because its sugar-binding a...
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PMID: 17621594
PDF is available here.
PDF is available here.
Abstract
We discuss our results with respect to the versatility of picocyanobacteria of the evolutionary lineage VI of cyanobacteria, and a habitat-related distribution pattern of Synechococcus genotypes....
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PMID: 17825073
PDF is available here.
PDF is available here.
Abstract
This study supports the efficacy of using FFC for the detection of viable protozoa in natural waters and indicates that use of RPE illuminated at 530 nm and detected at 585 nm provides a satisfactory means of attenuating background. SIGNIFICANCE AND IMPACT OF THE STUDY: Because of the severe global...
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PMID: 17714404
PDF is available here.
PDF is available here.
Abstract
We developed a method to realize a nanofluidic preconcentrator on a poly(dimethylsiloxane) (PDMS)-based microfluidic channel. The originality of our preconcentration device is the simple nanogap formation using the junction gap breakdown phenomenon between two PDMS microchannels, without using any p...
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PMID: 17628080
PDF is available here.
PDF is available here.
Abstract
The immunolabeling methods and the instrumentation are simple and easy-to-handle for less-trained operators. The SP ICM system is a good candidate for CD4(+) T lymphocytes enumeration in point-of-care settings of resource-constrained countries.
Copyright 2007 Clinical Cytometry Society....
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PMID: 17311352
PDF is available here.
PDF is available here.
Abstract
The adsorption properties of R-phycoerythrin (RPE), an autofluorescent protein, on the fused-silica surface were studied in capillary electrophoresis (CE) and in single-molecule experiments. The band shapes and migration times were measured in CE, and adsorption and desorption events were recorded a...
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PMID: 17658767
PDF is available here.
PDF is available here.
Abstract
We have studied a FRET-based homogeneous bioaffinity assay utilizing a tandem dye acceptor with a large pseudo-Stokes shift (139 nm). The tandem dye was constructed using B-phycoerythrin as an absorber and multiple Alexa Fluor 680 dyes as emitters. As a donor, we employed upconverting phosphor parti...
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PMID: 17628044
PDF is available here.
PDF is available here.
Journal of Chromatography A (1160)1-2 2007
Abstract
Miniaturized devices for electric field gradient focusing (EFGF) were developed that consist of a cylindrical separation channel surrounded by an acrylic-based polymer hydrogel. The ionic transport properties of the hydrogel enable the manipulation of the electric field inside the separation channel...
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PMID: 17481644
PDF is available here.
PDF is available here.
Abstract
Our results demonstrated that there were higher percentages of large granulocytes compared with hyalinocytes and the percentage of small granulocytes was related to the mitochondrial membrane potential and phagocytic activities....
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PMID: 17218115
PDF is available here.
PDF is available here.
FEMS Microbiology Letters (271)2 2007
Abstract
Members of the genus Prochlorococcus belong to the most abundant phytoplankton on earth. In contrast to other cyanobacteria, Prochlorococcus is characterized by divinyl-chlorophyll containing light-harvesting complexes and the lack of phycobilisomes. Despite the lack of phycobilisomes, all sequenced...
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PMID: 17439544
PDF is available here.
PDF is available here.
Analytical Biochemistry (365)1 2007
Abstract
The performance of two homemade fluorescence-induced capillary electrophoresis detectors, one based on light-emitting diode (LED) as the excitation source and a charge-coupled device (CCD) photodetector and the other based on a commercial luminescence spectrometer (Xe lamp) as the excitation source...
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PMID: 17391633
PDF is available here.
PDF is available here.
Photosynthesis Research (92)1 2007
Abstract
Our results suggest that D1 protein degradation was accompanied (or followed) by the disruption of the N-terminal domain of the anchor linker polypeptide LCM, which in turn led to the disconnection of the phycobilisome complex from the thylakoid membrane. Furthermore, time course analyses of in vivo...
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PMID: 17505911
PDF is available here.
PDF is available here.
Electrophoresis (28)7 2007
Abstract
This paper reports both the experimental application and 2-D simulation of ITP of proteins in a networked microfluidic chip. Experiments demonstrate that a mixture of three fluorescent proteins can be concentrated and stacked into adjacent zones of pure protein under a constant volta...
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PMID: 17330226
PDF is available here.
PDF is available here.
Abstract
We analyze patterns of phycoerythrin nucleotide variation in the HL- and LL-Prochlorococcus populations. Our analyses reveal a significantly greater number of non-synonymous fixed substitutions in peB and peA than expected based on interspecific comparisons. This pattern of excess non-synonymous fix...
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PMID: 16897463
PDF is available here.
PDF is available here.
Abstract
Porphyridium spp. is a red micro alga and is gaining importance as a source of valuable products viz., phycobiliproteins (PB), sulfated exopolysaccharides, and polyunsaturated fatty acids with potential applications in the food and pharmaceutical industries. In the present study, the effects of the...
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PMID: 17001641
PDF is available here.
PDF is available here.
Journal of Biochemistry (141)2 2007
Abstract
We were able to monitor the sugar-binding activity of VIP36 using less than 100 ng of the sVIP36-SA. This method is highly sensitive and suitable for detecting interactions between lectins and sugar chains of low affinity....
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PMID: 17169971
PDF is available here.
PDF is available here.
Abstract
Our results clearly show that the organic food products have an higher total antioxidant activity and bioactivity than the conventional foods. The results could be used in public health campaign to increase the consumption of products able to provide a significant health protection and prevention of...
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PMID: 17970235
PDF is available here.
PDF is available here.
Bioconjugate Chemistry (18)3 2007
Abstract
We investigated the application of luminescent nanoparticles as probes for Affymetrix GeneChips detection without the need for signal amplification. Our goal is to investigate the feasibility of using luminescent nanoparticles as probes in a commercial microarray system without changing its configur...
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PMID: 17447724
PDF is available here.
PDF is available here.
Journal of Physical Chemistry B (110)49 2006
Abstract
We report an investigation of energy migration dynamics in intact cells of the photosynthetic cryptophyte Rhodomonas CS24 using analyses of steady-state and time-resolved fluorescence anisotropy measurements. By fitting a specific model to the fluorescence data, we obtain three time scales (17, 58,...
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PMID: 17149931
PDF is available here.
PDF is available here.
Abstract
We developed a novel amphiphile (CholCSper) consisting of cholesterol linked to carboxy-spermine by a cysteine. This amphiphile is dimerizable upon mild oxidation of the thiol to disulfide and it was used in formulation with DOPE to prepare an intracellular protein delivery system. The stabilization...
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PMID: 17097756
PDF is available here.
PDF is available here.
Abstract
On normal B cells, the mean ABC value for unimolar CD20-PE conjugate was 143,500 (CV +/- 19.1%). The mean ABC value for B-CLL B-cells stained with the same conjugate was 21,700 (CV +/- 42.0%). Using the CD4 T-cell as a biologic calibrator for FITC conjugate, the mean ABC value for CD20-FITC on norma...
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PMID: 16967494
PDF is available here.
PDF is available here.
Abstract
In living organisms the photosynthesis involves the absorption of light by the light-harvesting (LH) antenna complexes. By mimicking the photosynthesis process the artificial photonic device composed of bio-molecular hetero structure is developed. The proposed photonic device is composed of Chl a, C...
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PMID: 17252804
PDF is available here.
PDF is available here.
Journal of Fluorescence (16)6 2006
Abstract
Molecular assays such as immunoassays are often performed using solid carriers and fluorescent labels. In such an assay format a question can be raised on how much the fluorescence of the label is influenced by the bio-affinity binding events and the solid carrier surface. Since changes in fluoresce...
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PMID: 16983509
PDF is available here.
PDF is available here.
Analytical Biochemistry (358)1 2006
Abstract
Formation of fluorescent proteins was explored after incubation of recombinant apo-subunits of phycobiliprotein R-phycoerythrin with phycoerythrobilin chromophore. Alpha and beta apo-subunit genes of R-phycoerythrin from red algae Polisiphonia boldii were cloned in plasmid pET-21d(+). Hexahistidine-...
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PMID: 16979575
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (281)37 2006
Abstract
Phycoerythrobilin is a linear tetrapyrrole molecule found in cyanobacteria, red algae, and cryptomonads. Together with other bilins such as phycocyanobilin it serves as a light-harvesting pigment in the photosynthetic light-harvesting structures of cyanobacteria called phycobilisomes. The biosynthes...
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PMID: 16857683
PDF is available here.
PDF is available here.
Abstract
R-phycoerythrin was isolated and purified from a red alga, Polysiphonia urceolata Grev, using Streamline column combined with ion-exchange chromatography or hydroxyapatite chromatography. The purity of R-phycoerythrin isolated by Streamline column was up to 1.66 and the yield of R-phycoerythrin coul...
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PMID: 16569506
PDF is available here.
PDF is available here.
Abstract
An integrated and simplified microfluidic device using a 250 microm x 1-4 cm of organic light emitting diode (OLED) array as a two-dimensional light source for single-channel and multichannel whole-column imaging detection was developed. This fluorescence detection system was used for isoelectric fo...
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PMID: 16906731
PDF is available here.
PDF is available here.
Journal of Bacteriology (188)9 2006
Abstract
We propose a likely process for type IV CA, which involves the enzymatic activity of one or several phycobilin lyases and/or lyase-isomerases differentially controlled by the ambient light quality. Phylogenetic analyses based on the 16S rRNA gene confirm that type IV CA is not limited to a single cl...
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PMID: 16621829
PDF is available here.
PDF is available here.
DNA Sequence (17)2 2006
Abstract
Phycobiliproteins function as a major light harvesting protein-pigment complex in the cyanobacteria and the eukaryotic algae. Phycoerythrin (PE) is a kind of phycobiliproteins, widely located in all rhodophytes, some species of cyanobacteria and cryptophytes, and different ecotypes of Prochlorococcu...
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PMID: 17076255
PDF is available here.
PDF is available here.
FEBS Letters (580)5 2006
Abstract
We developed a system to produce phytochromes with various chromophores in Escherichia coli. Through the selection of different bilin reductases, apophytochromes were assembled with phytochromobilin, phycocyanobilin, and phycoerythrobilin. The blue-shifted difference spectra of truncated phytochrome...
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PMID: 16458890
PDF is available here.
PDF is available here.