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Plastocyanin (0):
Recent article
Plastocyanin
Abstract
Plastocyanin and cytochrome c6 are widely distributed over the oxygen-evolving photosynthetic organisms. The two proteins are functionally equivalent, but strongly differ in their global electrostatic charge. In fact, they are acidic in eukaryotes, but either neutral or basic in cyanobacteria. Such...
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PMID: 20960123
PDF is available here.
PDF is available here.
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Plant Physiology (152)3 2010
Abstract
We conducted an extensive analysis of the plsp1-null mutant to address the significance of lumenal protein maturation in thylakoid development. Plastids that lack Plsp1 were found to accumulate vesicles of variable sizes in the stroma. Analyses of the mutant plastids revealed that the lack of Plsp1...
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PMID: 20097790
PDF is available here.
PDF is available here.
Abstract
An improved four-stage isolation and purification procedure for preparing poplar isoplastocyanins is described in detail. Absorbance (UV-VIS) spectroscopy and isoelectric focusing (IEF) are used to determine the protein purity and identity. The present procedure increases twice the total plastocyani...
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PMID: 20469642
PDF is available here.
PDF is available here.
Abstract
A multiparticle computer model of plastocyanin-cytochrome f complex formation in the thylakoid lumen has been designed, which takes electrostatic interactions of proteins and the thylakoid membrane into account. The Poisson-Boltzmann formalism was used to determine the electrostatic potential field...
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PMID: 20429279
PDF is available here.
PDF is available here.
Abstract
We report that PVXCP also interacts with the precursor of plastocyanin, a protein involved in photosynthesis, both in vitro and in vivo. Yeast two-hybrid analysis indicated that PVXCP interacted with only the plastocyanin transit peptide. In subsequent bimolecular fluorescence complementation assays...
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PMID: 19888818
PDF is available here.
PDF is available here.
Abstract
Oxidation of oxymyoglobin [MbO2(Fe2+)] by isoplastocyanins a (PCa) and b (PCb) was experimentally investigated and the corresponding redox reaction was modeled using the physicochemical parameters of the isoforms to study the effect of the dimorphism. The kinetic curve of oxidation of MbO2 (Fe2+) by...
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PMID: 20027868
PDF is available here.
PDF is available here.
Abstract
The redox potentials of poplar plastocyanins a and b (PCa, PCb) were determined by spectrophotometric titrations of their reduced forms with [Fe(CN)6]3-. It was found that the two isoforms have the following millimolar extinction coefficients epsilon597 equilibrium constants Keq of one-electron exch...
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PMID: 19678546
PDF is available here.
PDF is available here.
Abstract
Abstract
Two total plastocyanin (PC) fractions - loosely bound (lPC) and strongly bound (sPC) were extracted (84% and 16%, respectively) from the homogenate of Scenedesmus acutus MT8. Two-fold isolation-purification procedure including DE-52 chromatography separated IPC into a smaller oxidized [IPC (II)] and...
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PMID: 19069842
PDF is available here.
PDF is available here.
Photosynthesis Research (97)3 2008
Abstract
I (PSI) were grouped and compared. PsaA, psaB, psaC, and petG represent genes that have been retained in the chloroplasts of both green- and red-lineage species. PsaD, psaE, psaF, and petF represent genes that have been retained in the chloroplast of red-lineage species, but have been transferred to...
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PMID: 18661249
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1777)9 2008
Abstract
Our data also imply that the Gly8Asp mutation can diminish the magnitude of this change. The findings reported here are consistent with a reaction mechanism where the electron transfer in the complex between Pc and photosystem 1 is assumed to be reversible....
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PMID: 18445472
PDF is available here.
PDF is available here.
Abstract
We present a method that probes changes in the electric field at specific locations within a protein. The method utilizes the dependence of the amide (1)H and (15)N NMR chemical shifts on electric charges in proteins. Charges were introduced at different positions in the blue copper protein plastocy...
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PMID: 18214953
PDF is available here.
PDF is available here.
Abstract
We show here that when the data mentioned above are combined, it is possible to extract values of all of the parameters that characterize the exchange process, including rates, populations, and chemical shift changes, and to provide cross-validations that establish their accuracy. The methodology is...
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PMID: 18540585
PDF is available here.
PDF is available here.
Physiologia Plantarum (133)3 2008
Abstract
Persistent light quality gradients in dense plant populations induce imbalances in the distribution of excitation energy between the photosystems. Plants counteract such conditions by re-adjusting the stoichiometry of photosystems, which involves control of photosynthesis gene expression both in chl...
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PMID: 18419738
PDF is available here.
PDF is available here.
The Journal of Chemical Physics (128)22 2008
Abstract
We calculate the water-entropy gain upon the folding of apoPC using the angle-dependent integral equation theory combined with the multipolar water model and the recently developed morphometric approach. It is demonstrated that the calculated value is in quantitatively good accord with the value est...
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PMID: 18554061
PDF is available here.
PDF is available here.
Journal of Physical Chemistry A: Molecules, Spectroscopy, Kinetics, Environment and General Theory (112)17 2008
Abstract
Calculations of the g tensor of three copper(II) complexes [Cu(NH3)4]2+, [CuCl4]2-, and plastocyanin are presented. Two different sum-over-states-based approaches are considered, making use of the multistate CASPT2 method for excitation energies and PMCAS (perturbation modified CAS) wave functions f...
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PMID: 18386853
PDF is available here.
PDF is available here.
Journal of Physical Chemistry B (112)16 2008
Abstract
We report the results of molecular dynamics simulations of electron-transfer activation parameters of plastocyanin metalloprotein involved as an electron carrier in natural photosynthesis. We have discovered that slow, non-ergodic conformational fluctuations of the protein, coupled to hydrating wate...
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PMID: 18341321
PDF is available here.
PDF is available here.
The Journal of Chemical Physics (128)15 2008
Abstract
We report applications of analytical formalisms and molecular dynamics (MD) simulations to the calculation of redox entropy of plastocyanin metalloprotein in aqueous solution. The goal of our analysis is to establish critical components of the theory required to describe polar solvation at the mesos...
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PMID: 18433287
PDF is available here.
PDF is available here.
Abstract
We present the results of the first quantum chemical investigations of 1H NMR hyperfine shifts in the blue copper proteins (BCPs): amicyanin, azurin, pseudoazurin, plastocyanin, stellacyanin, and rusticyanin. We find that very large structural models that incorporate extensive hydrogen bond networks...
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PMID: 18314973
PDF is available here.
PDF is available here.
Abstract
The nature of transient protein complexes can range from a highly dynamic ensemble of orientations to a single well-defined state. This represents variation in the equilibrium between the encounter and final, functional state. The transient complex between plastocyanin (Pc) and cytochrome f (cytf) o...
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PMID: 18201089
PDF is available here.
PDF is available here.
Abstract
Equilibrium and nonequilibrium dynamics of a blue copper protein plastocyanin in an oxidized state are studied by molecular dynamics (MD) simulation. Potential energy functions of the lowest seven electronic states, including ligand-to-metal charge-transfer (LMCT) and copper d --> d excited states,...
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PMID: 18047310
PDF is available here.
PDF is available here.
Protein Science (10)12 2008
Abstract
A combination of site-directed mutagenesis and NMR chemical shift perturbation analysis of backbone and side-chain protons has been used to characterize the transient complex of the photosynthetic redox proteins plastocyanin and cytochrome f. To elucidate the importance of charged residues on complex...
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PMID: 11714931
PDF is available here.
PDF is available here.
Abstract
The diffusion of the protein plastocyanine and complex formation between plastocyanine and cytochrome f (a subunit of a cytochrome b6/f complex) in the chloroplast thylakoid lumen has been studied. A 3D computer simulation model of diffusion and binding of plastocyanine and cytochrome f has been con...
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PMID: 18543768
PDF is available here.
PDF is available here.
The Journal of Chemical Physics (127)17 2007
Abstract
These results indicated two-step hydrophobic collapses in the early stage of the apoPC folding, but the nature of the dynamics was different....
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PMID: 17994853
PDF is available here.
PDF is available here.
Journal of Physical Chemistry B (111)28 2007
Abstract
Modern density functional methods have been used to study spin-density distribution, g tensors, as well as Cu and ligand hyperfine tensors for azurin models, for two more blue copper proteins plastocyanin and stellacyanin, and for small model complexes. The aim was to establish a consistent computat...
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PMID: 17592871
PDF is available here.
PDF is available here.
Abstract
We investigated the adsorption characteristics, surface coverage and surface electric parameters of superstructures from two isoforms of plastocyanin, PCa and PCb, in an oxidized state adsorbed on beta-ferric hydrous oxide particles. The surface electric charge and electric dipole moments of the com...
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PMID: 17207973
PDF is available here.
PDF is available here.
Biochemical Journal (403)2 2007
Abstract
We have produced knockout mutants by chloroplast transformation in tobacco (Nicotiana tabacum). PsaJ binds two chlorophyll-a molecules and is localized at the periphery of PSI, close to both the Lhca2- and Lhca3-docking sites and the plastocyanin-binding site. Tobacco psaJ-knockout lines do not disp...
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PMID: 17209805
PDF is available here.
PDF is available here.
Abstract
We have produced this fern plastocyanin recombinantly and examined the properties of wild-type protein and mutants Phe12Leu, Gly36Pro, and the double mutant with NMR spectroscopy, X-ray crystallography, and cyclic voltammetry. The results demonstrate that, contrary to earlier reports, protonation of...
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PMID: 17367139
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1767)4 2007
Abstract
Electron transport processes were investigated in barley leaves in which the oxygen-evolution was fully inhibited by a heat pulse (48 degrees C, 40 s). Under these circumstances, the K peak (approximately F(400 micros)) appears in the chl a fluorescence (OJIP) transient reflecting partial Q(A) reduc...
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PMID: 17412308
PDF is available here.
PDF is available here.
Abstract
Our findings also make evident the fact that in Nostoc the main contribution to the electrostatic nature of the complex is provided by the small domain of cytochrome f....
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PMID: 17240983
PDF is available here.
PDF is available here.
Abstract
The ligand-containing loops of two copper-binding electron-transfer proteins (cupredoxins) have been swapped. In the azurin (AZ) variant in which the plastocyanin (PC) sequence is introduced (AZPC), the loop adopts a conformation identical to that in PC. The reduction potential of AZPC is raised as...
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PMID: 17227035
PDF is available here.
PDF is available here.
Photosynthesis Research (94)2-3 2007
Abstract
These results suggest that different PC conformations may exist in solution with different capabilities of forming electron-transfer-active docks. All three types of complexes show electrostatic contacts between D42, E43, and D44 on PC and K187 on cyt f as well as between E59 on PC and K58 on cyt f....
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PMID: 17972160
PDF is available here.
PDF is available here.
FEBS Letters (580)30 2006
Abstract
Perturbed angular correlation of gamma-rays (PAC) spectroscopy of cadmium substituted plastocyanin shows one dominant metal site configuration at pH 7.5. Lowering the pH to 4.8 a fraction of the molecules undergoes structural change and loses the bound cadmium ion. At pH 4.4 all plastocyanin is in t...
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PMID: 17161398
PDF is available here.
PDF is available here.
Abstract
We describe a rapid and simple method to express large amounts of a protein precursor in E. coli. We have analyzed the effect of ionophors as well as of azide on the accumulation of expressed protein precursors. Azide blocks the function of SecA and the ionophors dissipate the electrochemical gradie...
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PMID: 17129407
PDF is available here.
PDF is available here.
FEBS Letters (580)26 2006
Abstract
I are herein used as excellent model systems to investigate how the metal sites adapt to the changes in the protein matrix in transient complexes that are involved in redox reactions. Thus, both complexes from the cyanobacterium Nostoc sp. PCC 7119 (former Anabaena sp. PCC 7119) have been analysed b...
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PMID: 17064694
PDF is available here.
PDF is available here.
Abstract
Oxidized plastocyanin (PC) was reduced with TyrTyrTyr and LysLysLysLysTyrTyrTyr (KKKKYYY) oligopeptides at neutral pH. The TyrTyrTyr site of the peptides provided an electron to the copper active site of PC, whereas the tetralysine site of KKKKYYY functioned as the recognition site for the negative...
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PMID: 16963123
PDF is available here.
PDF is available here.
Photosynthesis Research (90)1 2006
Abstract
The transient complex between cytochrome f and plastocyanin from the cyanobacterium Nostoc sp. PCC 7119 has been analysed by X-ray Absorption Spectroscopy in solution, using both proteins in their oxidized and reduced states. Fe K-edge data mainly shows that the atypical metal coordination geometry...
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PMID: 17111237
PDF is available here.
PDF is available here.
Inorganic Chemistry (45)20 2006
Abstract
A wide variety of spectroscopic methods are now available that provide complimentary insights into the electronic structures of transition-metal complexes. Combined with calculations, these define key bonding interactions, enable the evaluation of reaction coordinates, and determine the origins of u...
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PMID: 16999398
PDF is available here.
PDF is available here.
Biophysical Journal (91)7 2006
Abstract
We also generated a model structure in which the FeS center of the Rieske protein was positioned closer to the heme of cyt f than observed in the crystal structure and studied its interactions with both PC and cyt c6. Our data showed that the Rieske protein in both the original crystal structure and...
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PMID: 16844750
PDF is available here.
PDF is available here.
Acta Crystallographica Section D (62)Pt 9 2006
Abstract
Plastocyanin from the cyanobacterium Anabaena variabilis was heterologously produced in Escherichia coli and purified. Plate-like crystals were obtained by crystallization in 1.15 M trisodium citrate and 7.67 mM sodium borate buffer pH 8.5. The crystals belong to the orthorhombic space group P2(1)2(...
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PMID: 16929103
PDF is available here.
PDF is available here.
Abstract
We propose a new breakdown of the copper hyperfine interaction into Fermi-contact, spin-dipolar and spin-orbit contributions....
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PMID: 16807973
PDF is available here.
PDF is available here.
Abstract
Two methods for estimating the microsecond-millisecond dynamics in proteins from only two 15N relaxation parameters at one magnetic field strength are investigated. Thus, the chemical exchange contribution, R(ex), to the transversal relaxation rate, which contains the dynamics information, is evalua...
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PMID: 16705625
PDF is available here.
PDF is available here.
Journal of Molecular Biology (360)5 2006
Abstract
We therefore suggest that the role of cytochrome c6A is to use its disulfide group to oxidize dithiol/disulfide groups of other proteins of the thylakoid lumen, followed by internal electron transfer from the dithiol to the heme, and re-oxidation of the heme by another thylakoid oxidant. Consistent...
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PMID: 16815443
PDF is available here.
PDF is available here.
Abstract
We here describe both experimental and computational studies of azurin, plastocyanin and stellacyanin designed to define more accurately the geometric structures of the active site of the reduced and oxidized species, and thus to understand how these structures determine the redox potentials of thes...
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PMID: 16786065
PDF is available here.
PDF is available here.
Abstract
A new method to investigate the initial protein folding dynamics is developed based on a pulsed laser light triggering method and a unique transient grating method. The side chain of the cysteine residue of apoplastocyanin (apoPC) was site-specifically modified with a 4,5-dimethoxy-2-nitrobenzyl der...
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PMID: 16756310
PDF is available here.
PDF is available here.
Physical Biology (3)2 2006
Abstract
We designed a 3D computer simulation model of diffusion and interaction of spinach plastocyanin and turnip cytochrome f in solution. It is the first step in simulating the electron transfer from cytochrome f to photosystem 1 in the lumen of thylakoid. The model is multiparticle and it can describe t...
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PMID: 16829698
PDF is available here.
PDF is available here.
Abstract
These results support the hypothesis that the pH of the thylakoid lumen has to remain moderate during steady-state photosynthesis and indicate that low pH induced aggregation of plastocyanin might serve as a regulatory switch for photosynthesis....
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PMID: 16570184
PDF is available here.
PDF is available here.
Abstract
We report that the greater Cu requirement in an oceanic diatom, Thalassiosira oceanica, is entirely due to a single Cu-containing protein, plastocyanin, which--until now--was only known to exist in organisms with chlorophyll b and cyanobacteria. Algae containing chlorophyll c, including the closely...
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PMID: 16572122
PDF is available here.
PDF is available here.
Abstract
Protein plastocyanin from a green alga, Ulva pertusa, has been purified. Samples were homogenized in 0.02 mol/L phosphate buffer (pH 7.2) and then centrifuged to remove debris and subjected to ammonium sulfate fractionation (40%-80% saturation). Ion exchange column chromatography with DEAE-Sepharose...
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PMID: 16929848
PDF is available here.
PDF is available here.
FEBS Letters (580)9 2006
Abstract
We propose that it catalyses the formation of disulphide bridges in thylakoid lumen proteins in a single-step disulphide exchange reaction, with subsequent transfer of the reducing equivalents to plastocyanin. The haem group of cytochrome c(6A) acts as an electron sink, allowing rapid resolution of...
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PMID: 16581069
PDF is available here.
PDF is available here.
Biochemistry (Washington) (45)15 2006
Abstract
The thermal unfolding of plastocyanin from the mesophilic cyanobacterium Synechocystis is described herein, and the results are compared with those obtained for the homologous thermophilic protein from Phormidium laminosum. The thermal unfolding is irreversible under all the conditions that were inv...
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PMID: 16605257
PDF is available here.
PDF is available here.
FEBS Letters (580)7 2006
Abstract
Autoreduction of Cucumis sativus plastocyanin and Pseudomonas aeruginosa azurin took place at alkaline pHs, having been accompanied by the decrease in the intensities of the charge transfer band, Cys-S- (pi)-->Cu(II) at 597 and 626 nm, and the Cu(II)-EPR signals with small AII values of 6.5 x 10(-3)...
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PMID: 16500649
PDF is available here.
PDF is available here.
Abstract
We find certain sets of consecutive strands that are connected by hydrogen bonds, which we call "strandons." The analysis of the arrangements of strandons in 491 protein structures that come from 69 different superfamilies reveals strict regularities in the arrangements of strandons and the formatio...
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PMID: 16537492
PDF is available here.
PDF is available here.
Abstract
The utility of pseudocontact shifts in the structure refinement of metalloproteins has been evaluated using a native, paramagnetic Cu(2+) metalloprotein, plastocyanin from Anabaena variabilis (A.v.), as a model protein. First, the possibility of detecting signals of nuclei spatially close to the par...
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PMID: 16477687
PDF is available here.
PDF is available here.
Journal of Physical Chemistry A: Molecules, Spectroscopy, Kinetics, Environment and General Theory (110)7 2006
Abstract
The spectroscopic properties of the self-associated complexes formed between the anionic surface docking site of spinach plastocyanin and the cationic metalloporphyrins, in which the tyrosine 83 (Y83) moiety is placed just below the docking site, tetrakis(N-methyl-4-pyridyl)porphyrin (Pd(II)TMPyP(4+...
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PMID: 16480316
PDF is available here.
PDF is available here.
Abstract
We show that the geometric structure of the metal site of a metalloprotein in solution can be determined from experimentally measured electron-nuclear spin-spin interactions obtained by NMR. Thus, the geometric metal site structure of plastocyanin from Anabaena variabilis was determined by including...
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PMID: 16446449
PDF is available here.
PDF is available here.
Abstract
The synthesis and structure of a homologous series of cationic N(2)S(2) copper(I) Schiff base complexes constructed using o-tert-butylthiobenzaldehyde and a series of terminal diamines (ethane, propane, butane) are reported. The complexes differ only in the length of the methylene chain between the...
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PMID: 16387361
PDF is available here.
PDF is available here.
Abstract
We have tested the relevance of such kinds of interactions in the photosystem I reduction inside the cell. Several plastocyanin and cytochrome c6 site-directed mutant strains affecting both the acidic and hydrophobic regions of the two metalloproteins, which were previously characterized in vitro, h...
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PMID: 16411782
PDF is available here.
PDF is available here.
Biophysical Journal (90)2 2006
Abstract
We were able to attribute this to structural differences among these molecules, particularly to a small flexible loop between A-184 and G-191 (which has some of the highest crystallographic temperature factors in all of the cyt f structures) on the cyt f small domain. We also showed that deletion of...
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PMID: 16239335
PDF is available here.
PDF is available here.
Abstract
Plastocyanin was purified from the gymnosperm Ginkgo biloba L., and its complete amino acid sequence was determined. The protein was shown to contain Phe-83 instead of Tyr-83 conserved in other land plant plastocyanins. This is the first report of the characterization and complete amino acid sequenc...
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PMID: 16454664
PDF is available here.
PDF is available here.