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Ribosome Inactivating Proteins (2):
Recent article
Ribosome Inactivating Proteins
Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences (35)6 2010
Abstract
To detect the expression of galectin-3 (gal-3) and Sambucus nigra agglutinin (SNA) and determine their clinicopathological significance in breast cancers and benign breast lesions.
Envison immunohistochemistry for staining gal-3 expression, and ABC affinity-cytochemis...
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PMID: 20622331
PDF is available here.
PDF is available here.
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Physiologia Plantarum (138)3 2010
Abstract
The suggested link between intracellular cytokinin signaling and phospholipase D (PLD, EC 3.1.4.4.) activity (Romanov et al. 2000, 2002) was investigated. The activity of PLD in the early period of cytokinin action was studied in vivo in derooted Amaranthus caudatus seedlings, using...
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PMID: 19961548
PDF is available here.
PDF is available here.
Physiologia Plantarum (138)3 2010
Abstract
The suggested link between intracellular cytokinin signaling and phospholipase D (PLD, EC 3.1.4.4.) activity (Romanov et al. 2000, 2002) was investigated. The activity of PLD in the early period of cytokinin action was studied in vivo in derooted Amaranthus caudatus seedlings, using...
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PMID: 19961548
PDF is available here.
PDF is available here.
Physiologia Plantarum (138)3 2010
Abstract
The suggested link between intracellular cytokinin signaling and phospholipase D (PLD, EC 3.1.4.4.) activity (Romanov et al. 2000, 2002) was investigated. The activity of PLD in the early period of cytokinin action was studied in vivo in derooted Amaranthus caudatus seedlings, using...
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PMID: 19961548
PDF is available here.
PDF is available here.
Physiologia Plantarum (138)3 2010
Abstract
The suggested link between intracellular cytokinin signaling and phospholipase D (PLD, EC 3.1.4.4.) activity (Romanov et al. 2000, 2002) was investigated. The activity of PLD in the early period of cytokinin action was studied in vivo in derooted Amaranthus caudatus seedlings, using...
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PMID: 19961548
PDF is available here.
PDF is available here.
Abstract
We propose that these cancer-associated glycan variants be exploited for biomarker development aimed at diagnosing early-stage disease. Accordingly, we developed a mass spectrometry-based workflow that incorporates chromatography on affinity matrices formed from lectins, proteins that bind specific...
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PMID: 19798022
PDF is available here.
PDF is available here.
Abstract
We investigated the glycosylation signature, as well as the regulated expression of galectin-1 and -3 in human duodenal samples of allergic and non-allergic children. Whereas galectin-1 was predominantly localized in the epithelial compartment (epithelial cells and intraepithelial lymphocytes) and t...
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PMID: 19309568
PDF is available here.
PDF is available here.
Phytochemistry (69)17 2008
Abstract
I (SNA-I) is a type-2 RIP, isolated from the bark of elderberry (S. nigra L.). This study demonstrated the insecticidal potency of SNA-I on two Hemipteran insect species using two different methods. An artificial diet supplemented with different concentrations of the purified RIP reduced survival an...
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PMID: 18951590
PDF is available here.
PDF is available here.
Toxicology In Vitro (22)8 2008
Abstract
Our results clearly show oxidative DNA damage potential of abrin at low concentration....
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PMID: 18854210
PDF is available here.
PDF is available here.
Abstract
Ribosome inactivating proteins (RIPs) are enzymes that inactivate ribosomes by eliminating one or more adenosine residues from rRNA, a 9,567-Da RIP with a novel N-terminal sequence was isolated from fresh fruiting bodies of the mushroom Hypsizigus marmoreus. The protein was unadsorbed on DEAE-cellul...
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PMID: 18751697
PDF is available here.
PDF is available here.
Journal of Biochemistry (144)4 2008
Abstract
These results suggest that the structure of alpha-momorcharin holds the more compact conformation as an incipient state for unfolding, which is the sharp contrast to beta-momorcharin that gives the characteristics of the generally known molten globule state....
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PMID: 18603588
PDF is available here.
PDF is available here.
Abstract
We compared the capacities of the RIP ricin to promote 28S ribosomal RNA (rRNA) cleavage with that of the trichothecenes, deoxynivalenol (DON), and T-2 toxin (T-2). In a cell-free model, exposure to ricin at 300 ng/ml for 30 min depurinated yeast 28S rRNA, however, neither DON (< or = 4 microg/ml) n...
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PMID: 18535001
PDF is available here.
PDF is available here.
Abstract
We review quorum sensing inhibitors as potential therapeutics for even the most persistent infections. The animal models reviewed are subcutaneous graft, central venous catheter (CVC), ureteral stent and wound models, and a wound case study. The therapeutic approaches reviewed are the use of RNAIII...
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PMID: 18924087
PDF is available here.
PDF is available here.
Analytical Biochemistry (378)1 2008
Abstract
A simple electrochemiluminescence-based assay for RNA N-glycosidase activity has been modified to permit its use with authentic extracts of Ricinus communis (castor beans) and Abrus precatorius (jequirity seeds)--the natural sources of ricin and abrin. Modifications include the addition of an RNase...
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PMID: 18394420
PDF is available here.
PDF is available here.
Abstract
We report here the fabrication of a novel sensing interface of biotinylated sialosides to probe lectin-carbohydrate interactions using surface plasmon resonance spectroscopy (SPR). The attachment of carbohydrates to the surface using biotin-NeutrAvidin interactions and the implementation of an inert...
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PMID: 18461973
PDF is available here.
PDF is available here.
Abstract
We first metabolically radiolabelled non-mating-competent cells with D-[6-(3)H]galactose, and then analyzed the radiolabelled proteins by anion exchange chromatography. We characterized a 38 kDa (gp38) sialic acid-containing glycoprotein and raised the corresponding polyclonal antibody by means of w...
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PMID: 17870426
PDF is available here.
PDF is available here.
Abstract
We show that TCS specifically depurinate the predicted A(51) residue on 28Sdelta. We also demonstrated that the C-terminal end of ribosomal P proteins is needed for full activity of the toxin. In contrast to TCS, PAP inactivated efficiently T.cruzi ribosomes, and most importantly, does not require f...
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PMID: 18282466
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1780)5 2008
Abstract
We purified a protein showing RIP activity from Arabidopsis thaliana by employing chromatography separations coupled with an enzymatic activity. Based on N-terminal and internal amino acid sequencing, the RIP purified was identified as a mature form of pectin methylesterase (PME, At1g11580). The pur...
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PMID: 18222123
PDF is available here.
PDF is available here.
Abstract
The ribosome inactivating protein PD-L4 from Phytolacca dioica is a N-beta-glycosidase, probably involved in plant defence. The crystal structures of wild type PD-L4 and of the S211A PD-L4 mutant with significantly decreased catalytic activity were determined at atomic resolution. To determine the s...
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PMID: 17963235
PDF is available here.
PDF is available here.
Abstract
In Africa, approximately 25 million pregnant women are at risk of Plasmodium falciparum infection each year, one in four has evidence of placental involvement and up to half of these may be associated with low birth weight outcomes. In infected pregnant women, the placenta is an ideal site for the a...
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PMID: 18243302
PDF is available here.
PDF is available here.
Abstract
We report on the induction of NO synthesis in a murine macrophage cell line, RAW264.7, by Korean mistletoe lectin (KML-IIU). When the macrophage cells were treated with KML-IIU in the presence of a suboptimal concentration of IFN-gamma, NO production was induced in a concentration-dependent manner....
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PMID: 17940853
PDF is available here.
PDF is available here.
Abstract
A full-length cDNA encoding ribosome-inactivating/antiviral protein (RIP/AVP)from the leaves of Bougainvillea x buttiana was isolated.The cDNA consisted of 1364 nucleotides with an open reading frame (ORF)of 960 nucleotides encoding a 35.49 kDa protein of 319 amino acids.The deduced amino acid seque...
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PMID: 18376074
PDF is available here.
PDF is available here.
Biochemistry (New York) (73)3 2008
Abstract
A full-length cDNA encoding ribosome-inactivating/antiviral protein from the leaves of Bougainvillea xbuttiana was recently isolated. The coding region of cDNA was cloned and expressed in Escherichia coli, and the protein product was designated as BBAP1 (Bougainvillea xbuttiana antiviral protein 1)....
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PMID: 18393761
PDF is available here.
PDF is available here.
Planta Medica (74)2 2008
Abstract
Extracts of Viscum album L. produced by a specific homogenization procedure contain viscotoxins (VT) and liposome-like membrane vesicles, formed from cellular membranes. Interactions between these membrane structures and viscotoxins are characterized in this work. Binding properties of viscotoxins w...
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PMID: 18203060
PDF is available here.
PDF is available here.
Amino Acids (34)2 2008
Abstract
Cinnamomin from Cinnamonum camphora seeds, a type II ribosome-inactivating protein that interferes with protein biosynthesis in mammalian cells, can induce the apoptosis of carcinoma cells and be used as an insecticide. A rapid and improved method has been developed for the extraction and purificati...
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PMID: 17404804
PDF is available here.
PDF is available here.
Abstract
BE27 and BE29 are two forms of beetin, a virus-inducible type 1 ribosome-inactivating protein isolated from leaves of Beta vulgaris L. Western blot analysis revealed the presence of beetin forms in adult plants but not in germ or young plants, indicating that the expression of these proteins is deve...
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PMID: 18343888
PDF is available here.
PDF is available here.
Abstract
Abstract
We cloned a novel protein from Trichosanthes kirilowii Maximowicz (Family Cucurbitaceae) named TYchi. Expression of TYchi gene in T. kirilowii plants was induced by F. oxysporum, an important cucurbitaceous fungal pathogen, which indicated that TYchi involved in the pathogen-induced plant defense re...
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PMID: 17981782
PDF is available here.
PDF is available here.
Abstract
Two isolectins (KML-IIU and the KML-IIL) were individually isolated from the previously reported Korean mistletoe lectin, KML-C, by using an immunoaffinity column. Molecular weights of the KML-IIU and the KML-IIL were 64 kDa and 60 kDa respectively. Both of the lectins were composed of heterogeneous...
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PMID: 18047792
PDF is available here.
PDF is available here.
Biomolecular Engineering (24)6 2007
Abstract
I ribosome-inactivating protein (RIP) with multiple biological and pharmacological activities. It has been approved effective in the clinical treatment of AIDS and tumor, but its strong immunogenicity and short plasma half-life have limited the clinical administration. To reduce the immunogenicity a...
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PMID: 18023612
PDF is available here.
PDF is available here.
Abstract
Cassin, the new gene of ribosome-inactivating protein (RIP) isolated from Cassia occidentalis, was inserted into expression vector pBI121 to produce plant expression vector pBI121-cassin (Figs.1, 2). pBI121-cassin was introduced into tobacco cultivar 'K326' by the Agrobacteriurm tumefaciens transfor...
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PMID: 18349505
PDF is available here.
PDF is available here.
Biochemical Journal (408)1 2007
Abstract
We provide evidence that its expression is confined to proliferating cells through dependence on the Rb (retinoblastoma protein) family/E2F cell cycle pathway. Mutation of the Rb or E2F1 genes lead to induction of ZFHX1A mRNA, implying that the Rb-E2F1 repressor complex is important for repression o...
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PMID: 17655524
PDF is available here.
PDF is available here.
Biotechnology Letters (29)10 2007
Abstract
The gene encoding a single-chain, ribosome-inactivating protein (SCRIP) was cloned from bitter melon (Momordica charantia L.) leaves infected with the fungus, Sphaerotheca fuliginea, by RT-PCR. The ORF was 861 bp. The ribosome-inactivating protein was expressed in E. coli and, when purified, it inhi...
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PMID: 17579811
PDF is available here.
PDF is available here.
Journal of Biochemistry (142)3 2007
Abstract
We tried to identify the amino-acid residues in the Japanese elderberry bark lectin, Sambucus sieboldiana agglutinin that enabled the lectin to recognize sialic acid by using in silico docking simulation and site-directed mutagenesis. These studies showed that three amino-acid residues, S(197), A(23...
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PMID: 17646175
PDF is available here.
PDF is available here.
Abstract
We demonstrated, for the first time, that non-muscle myosin heavy chain IIA (NMHC-IIA) was cleaved by VAA-I treatment. Degradation of NMHC-IIA was reversed by the pan caspase inhibitor z-VAD-fmk in PLB-985D cells and neutrophils. However, unlike lamin B(1), no NMHC-IIA was detected on the cell surfa...
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PMID: 17659056
PDF is available here.
PDF is available here.
Abstract
We present here a novel strategy for small scale cell purifications using lectin-modified microchannels. Mammalian cells can be selectively captured in lectin-modified channels and eluted with a solution of the lectin's inhibiting sugar. As a proof-of-principle demonstration, two cell lines with dif...
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PMID: 17516171
PDF is available here.
PDF is available here.
Oncology Reports (17)5 2007
Abstract
Our present results suggest that decrease in sialylation rather than increase is a characteristic feature of malignant transformation in the thyroid....
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PMID: 17390071
PDF is available here.
PDF is available here.
Ukrainskij biokhimicheskij zhurnal/Ukrayins'kij biokhimichnij zhurnal/Ukrainian biochemical journal (79)5 2007
Abstract
Isolation of lectins from extracts of the Sambucus nigra inflorescences and of pollen material have been performed using isoelectric focusing without carrier ampholytes (autofocusing). Fractions active in agglutination tests with different carbohydrate specificity were subjected to SDS-PAGE. The maj...
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PMID: 18357788
PDF is available here.
PDF is available here.
Nucleic Acids Research (35)18 2007
Abstract
Our structure suggests that maize ribosome-inactivating protein may represent an intermediate product in the evolution of ribosome-inactivating proteins....
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PMID: 17855394
PDF is available here.
PDF is available here.
Abstract
PD-L1 is a highly glycosylated type 1 ribosome inactivating protein, from Phytolacca dioica leaves, with the peculiarity to act also as a DNase. PD-L1 has been successfully crystallized using vapour diffusion and seeding techniques. Crystals belong to the monoclinic C2 space group, with unit cell di...
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PMID: 17504100
PDF is available here.
PDF is available here.
Abstract
We show that the C-terminal pentapeptide (KISIA) and the GNIFRGF internal sequence fused at the C terminal region of genes encoding secretory versions of green fluorescent protein (GFP) and GFP-beta-glucuronidase (GFP-GUS) were sufficient to redirect these reporter proteins to the vacuole of Arabido...
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PMID: 17466526
PDF is available here.
PDF is available here.
Journal of Biochemistry (141)1 2007
Abstract
These results suggest that the transient folding/unfolding intermediate state of beta-momorcharin adopt the specific conformation at the vicinity of the active site, although it exhibits very similar properties with those of the generally known molten-globule state....
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PMID: 17167047
PDF is available here.
PDF is available here.
Abstract
Our conclusion is that open surgery decreased the level of IGFBP-3 compared with laparoscopy, whereas patients with gallbladder inflammation that underwent laparoscopy had an increased content of sialic acid in IGFBP-3....
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PMID: 17191668
PDF is available here.
PDF is available here.
Abstract
Pseudomonas aeruginosa is a pathogen gaining prevalence in contact lens-related corneal ulcers. Tear outflow protects the ocular surface, where high molecular weight tear glycoproteins bind bacteria for removal from the eye. The purpose of the present study was to identify glycoproteins in human tea...
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PMID: 16844114
PDF is available here.
PDF is available here.
Analytical Biochemistry (357)2 2006
Abstract
Synthetic biotinylated RNA substrates were cleaved by the combined actions of ricin holotoxin and a chemical agent, N,N'-dimethylethylenediamine. The annealing of the product with a ruthenylated oligodeoxynucleotide resulted in the capture of ruthenium chelate onto magnetic beads, enabling the elect...
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PMID: 16942744
PDF is available here.
PDF is available here.
Abstract
We aim to understand and define the molecular basis and biological effects of the type II RIPs, through the studies of the recombinant Korean mistletoe lectin. To this end, we expressed, purified the recombinant Korean mistletoe lectin (rKML), and investigated its molecular characteristics in vitro,...
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PMID: 17002877
PDF is available here.
PDF is available here.
Glycobiology (16)10 2006
Abstract
We obtained evidence for two sites showing dissimilar binding affinity. Intriguingly, the site with higher affinity was only partially occupied. To assign the observed properties to the Trp/Tyr-sites, we next performed chemically induced dynamic nuclear polarization measurements of Trp and Tyr acces...
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PMID: 16774910
PDF is available here.
PDF is available here.
Journal of Bacteriology (188)19 2006
Abstract
Deinococcus geothermalis E50051 forms tenuous biofilms on paper machine surfaces. Field emission electron microscopy analysis revealed peritrichous appendages which mediated cell-to-surface and cell-to-cell interactions but were absent in planktonically grown cells. The major protein component of th...
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PMID: 16980504
PDF is available here.
PDF is available here.
Biological Chemistry (387)9 2006
Abstract
The complete amino acid sequence of lychnin, a type 1 ribosome-inactivating protein (RIP) isolated from Lychnis chalcedonica seeds, has been determined by automated Edman degradation and ESI-QTOF mass spectrometry. Lychnin consists of 234 amino acid residues with a molecular mass of 26 131.14 Da. Al...
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PMID: 16972795
PDF is available here.
PDF is available here.
Abstract
The cytotoxicity of intact cinnamomin (a type II ribosome-inactivating protein, RIP) and the RNA N-glycosidase activity of cinnamomin A-chain have been studied and compared with those of ricin. Cinnamomin A-chain exhibits a similar RNA N-glycosidase activity in inhibiting in vitro protein synthesis...
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PMID: 16762311
PDF is available here.
PDF is available here.
Abstract
We modified the reported method for the prediction of antigenic site and identified two crucial amino acid residues (Y55 and D78) for a new epitope. We mutated these two residues into glycine and serine, respectively, and obtained three mutants, Y55G, D78S, and Y55G/D78S. These mutants induced less...
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PMID: 16581017
PDF is available here.
PDF is available here.
Abstract
A variety of studies have shown that incubation of different tumour cell lines with mistletoe lectins (MLs) in vitro has a marked cytotoxic effect. In the concentration range of low cytotoxicity cell death induced by ML-I is quantitatively due to apoptotic processes. The first events observed being...
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PMID: 16372250
PDF is available here.
PDF is available here.
Abstract
A practical and convenient method for discriminating between the presence and the absence of sialic acid in carbohydrate chains of glycoproteins was devised using paramagnetic beads and two lectins, Sambucus sieboldiana lectin (SSA) and Ricinus communis agglutinin (RCA120). The glycoproteins of tran...
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PMID: 16196055
PDF is available here.
PDF is available here.
Abstract
Leukemia Research (29)12 2005
Abstract
We demonstrated that lamin B(1) is a novel target to VAA-I and its degradation was reversed by a pan-caspase inhibitor and by a caspase-6, but not a caspase-8, inhibitor....
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PMID: 16242777
PDF is available here.
PDF is available here.
Abstract
Beta-Secretase (betaSEC) was expressed in Trichoplusia ni BTI Tn5B1-4 (Tn5B1-4) cells transformed with cDNAs encoding beta1,4-galactosyltransferase (GalT) and Gal beta1,4-GlcNAc alpha 2,6-sialyltransferase (ST). The apparent molecular weight of recombinant beta-secretase was increased from 57 to 59...
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PMID: 16226891
PDF is available here.
PDF is available here.
Abstract
We investigated whether the pro-apoptotic agent Viscum album agglutinin-I (VAA-I) could induce eosinophil apoptosis. VAA-I was found to induce apoptosis in eosinophilic AML14.3D10 (3D10) cells and that these cells expressed caspases-1, -2, -3, -4, -7, -8, -9 and -10. VAA-I-induced gelsolin degradati...
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PMID: 16098066
PDF is available here.
PDF is available here.
Abstract
Silica-based lectin microcolumns are described in this study together with the chemical procedures necessary for their preparation. The analytical merits of Canavalia ensiformis and Sambucus nigra lectins, [immobilized on activated macroporous silica], such as binding capacity, trapping reproducibil...
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PMID: 15987113
PDF is available here.
PDF is available here.
Analytical Biochemistry (342)1 2005
Abstract
Abstract
The primary structure of the B chain of the N-acetyl-D-galactosamine-recognizing mistletoe lectin-3 (ML-3B) has been deduced from proteolytic digest peptides of the purified glycoprotein, their HPLC-separation and Edman degradation and confirmation of the peptide sequences by MALDI-MS. ML-3B consist...
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PMID: 15635663
PDF is available here.
PDF is available here.