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Plant Proteins (16):
Recent article
Plant Proteins
Journal of Proteomics (74)8 2011
Abstract
We explore the stromal proteome of spinach and Arabidopsis chloroplast and show that clustering of mass spectra is a useful tool for investigating such variants and detecting modified peptides with amino acid substitutions or post-translational modifications. This study employs data mining by hierar...
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PMID: 21539947
PDF is available here.
PDF is available here.
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Journal of Proteomics (74)8 2011
Abstract
Salinity in agricultural land is a major problem worldwide, placing a severe constraint on crop growth and productivity in many regions, and increased salinization of arable land is expected to have devastating global effects. Though plants vary in their sensitivity to salt stress, h...
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PMID: 21440686
PDF is available here.
PDF is available here.
Journal of Proteomics (74)8 2011
Abstract
We have analyzed the adaptive responses of Lupinus albus roots to long-term B deficiency. Large morphological differences were observed between plants grown with or without B, and 265 polypeptides were found to be responsive to B deficiency out of a total of 406 polypeptides detected by two-dimensio...
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PMID: 21406259
PDF is available here.
PDF is available here.
Journal of Proteomics (74)8 2011
Abstract
In common with other aerobic organisms, plants are exposed to reactive oxygen species resulting in formation of post-translational modifications related to protein oxidoreduction (redox PTMs) that may inflict oxidative protein damage. Accumulating evidence also underscores the import...
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PMID: 21406256
PDF is available here.
PDF is available here.
Journal of Proteomics (74)8 2011
Abstract
The workhorse for proteomics in non-model plants is classical two-dimensional electrophoresis, a combination of iso-electric focusing and SDS-PAGE. However, membrane proteins with multiple membrane spanning domains are hardly detected on classical 2-DE gels because of their low abund...
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PMID: 21354347
PDF is available here.
PDF is available here.
Journal of Proteomics (74)8 2011
Abstract
Membrane proteins are an interesting class of proteins because of their functional importance. Unfortunately their analysis is hampered by low abundance and poor solubility in aqueous media. Since shotgun methods are high-throughput and partly overcome these problems, they are prefer...
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PMID: 21334471
PDF is available here.
PDF is available here.
Journal of Proteomics (74)8 2011
Abstract
Plant acclimation to stress is associated with profound changes in proteome composition. Since proteins are directly involved in plant stress response, proteomics studies can significantly contribute to unravel the possible relationships between protein abundance and plant stress acc...
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PMID: 21329772
PDF is available here.
PDF is available here.
Abstract
The proteome of untreated white wines (a Recioto made with Garganega grapes from the Veneto region) was explored in depth via capture with combinatorial peptide ligand libraries (CPLL) at four different pH values: pH 2.2, 3.8, 7.2, and 9.3. The combined data on the discoveries in the...
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PMID: 21702502
PDF is available here.
PDF is available here.
Abstract
We have evaluated here the capacity of DMTI-II to enhance the allergic pulmonary inflammation, looking over time to the leukocyte trafficking from bone marrow to peripheral blood, and their recruitment into the allergic airways. Male Wistar rats were sensitized and challenged with ovalbumin (OVA). A...
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PMID: 21315171
PDF is available here.
PDF is available here.
Plant Biology (62)1 2011
Abstract
The epigenomic regulation of chromatin structure and genome stability is essential for the interpretation of genetic information and ultimately the determination of phenotype. High-resolution maps of plant epigenomes have been obtained through a combination of chromatin technologies...
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PMID: 21438682
PDF is available here.
PDF is available here.
Proteomics (11)12 2011
Abstract
We also found differences at post-translational level. The results indicated that late embryogenesis abundant Rab21 was more strongly phosphorylated in the embryos of the sensitive varieties than in the embryos of the tolerant ones. We propose that the differences found in the phosphorylation status...
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PMID: 21595035
PDF is available here.
PDF is available here.
Abstract
Cold acclimation is the phenomenon in which plants are exposed to low, but nonfreezing, temperatures before exposure to drastic temperatures. To investigate how sunflower plants adjust their metabolism during cold treatment, a comparative proteomic approach, based on spectral countin...
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PMID: 21341810
PDF is available here.
PDF is available here.
Abstract
We use stem-loop real-time reverse transcription-polymerase chain reaction to quantify the expression changes of seven miRNAs and their target mRNAs in different tomato tissues during the pathogenic processes. Compared with mock-inoculated plants, the expression levels of investigated miRNAs and mRN...
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PMID: 21335334
PDF is available here.
PDF is available here.
Proteomics (11)8 2011
Abstract
We describe a strategy to enrich for and identify secreted plant proteins based on affinity chromatography using the lectin Concanavalin A and two-dimensional liquid chromatography, together with matrix-assisted laser desorption/ionization MS analysis. The value of this approach is illustrated throu...
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PMID: 21381198
PDF is available here.
PDF is available here.
Proteomics (11)7 2011
Abstract
We developed a two-step strategy for the enrichment of low-abundant soluble chloroplast proteins from Pisum sativum and their subsequent identification by MS. First, chloroplast protein extracts were depleted from the most abundant protein ribulose-1,5-bisphosphate carboxylase/oxygenase by SEC or he...
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PMID: 21365755
PDF is available here.
PDF is available here.
Abstract
We propose that cell wall restructuring and superior homeostasis, particularly the management of reactive oxygen species, may render better dehydration-adaptation. To our knowledge, this is the first report on the comprehensive comparison of dehydration-responsive organellar proteome of two genotype...
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PMID: 21348435
PDF is available here.
PDF is available here.
Abstract
To establish an efficient and high resolution 2-DE (two-dimensional electrophoresis) protocol for root tuber proteome analysis of Rehmannia glutinosa.
Proteins from root tuber of R. glutinosa were extracted by using five different methods and their productivity and pr...
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PMID: 21809567
PDF is available here.
PDF is available here.
Cell Host & Microbe (9)3 2011
Abstract
We find that HopZ1 physically interacts with the isoflavone biosynthesis enzyme, 2-hydroxyisoflavanone dehydratase (GmHID1). P. syringae infection induces gmhid1 expression and production of daidzein, a major soybean isoflavone. Silencing gmhid1 increases susceptibility to P. syringae infection, su...
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PMID: 21402357
PDF is available here.
PDF is available here.
Cell Host & Microbe (9)3 2011
Abstract
We determine the crystal structure of the L6 TIR domain and show that, although dispensable for pathogenic effector protein recognition, the TIR domain alone is both necessary and sufficient for L6 immune signaling. We demonstrate that the L6 TIR domain self-associates, most likely forming a homodi...
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PMID: 21402359
PDF is available here.
PDF is available here.
Journal of Proteomics (74)3 2011
Abstract
Plant pathogenic fungi cause important yield losses in crops. A proteomic approach was used to study the changes in the leaf proteome profile of the plant Mentha arvensis infected with a necrotrophic fungus, Alternaria alternata. High-resolution two-dimensional gel electrophoresis (2...
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PMID: 21111074
PDF is available here.
PDF is available here.
Molecular Plant (4)2 2011
Abstract
Polysaccharide-rich plant cell walls are important biomaterials that underpin plant growth, are major repositories for photosynthetically accumulated carbon, and, in addition, impact greatly on the human use of plants. Land plant cell walls contain in the region of a dozen major poly...
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PMID: 21199879
PDF is available here.
PDF is available here.
Journal of Proteomics (74)3 2011
Abstract
The role of salicylic acid (SA) in inducing resistance to MYMIV infection in Vigna mungo has been elucidated by proteomics. Twenty-nine proteins identified by MALDI-TOF/TOF, predicted to be involved in stress responses, metabolism, photosynthesis, transport and signal transduction, s...
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PMID: 21130907
PDF is available here.
PDF is available here.
Abstract
The growing importance of vesicular trafficking and cytoskeleton dynamic reorganization during plant development requires the exploitation of novel experimental approaches. Several genetic and cell biological studies have used diverse pharmaceutical drugs that inhibit vesicular traff...
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PMID: 21090759
PDF is available here.
PDF is available here.
Proteomics (11)3 2011
Abstract
We hypothesized that the RCN rice might fail to maintain sugar nutrient homeostasis. This was confirmed with the observation that the sucrose concentration was increased significantly in the RCN rice compared with the wild-type rice. Also, the RCN rice showed a hypersensitive response to exogenous s...
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PMID: 21268274
PDF is available here.
PDF is available here.
Proteomics (11)3 2011
Abstract
We undertook a proteomic analysis of N. tabacum trichomes in order to identify their protein complement. Efficient trichome isolation was obtained by abrading frozen leaves. After homogenization, soluble proteins and a microsomal fraction were prepared by centrifugation. Gel-based and gel-free prote...
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PMID: 21268273
PDF is available here.
PDF is available here.
Proteomics (11)3 2011
Abstract
Grains of hexaploid wheat, Triticum aestivum (cv. Récital), were collected at 15 stages of development, from anthesis to physiological maturity, 0-700°C days (degree days after anthesis). Two hundred and seven proteins of grain peripheral layers (inner pericarp, hyaline, testa and...
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PMID: 21268267
PDF is available here.
PDF is available here.
Journal of Proteomics (74)2 2011
Abstract
We report here a proteomic analysis of bean seeds by two-dimensional electrophoresis (2-DE). Three different protein extraction methods (TCA-acetone, phenol and the commercial clean-up kit) were used taking into account that the extractome can have a determinant impact on the level of quality of dow...
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PMID: 20971221
PDF is available here.
PDF is available here.
Abstract
Human milk contains a wide variety of nutrients that contribute to the fulfillment of its functions, which include the regulation of newborn development. However, few studies have investigated the concentrations of S100B protein, brain-derived neurotrophic factor (BDNF), and glial ce...
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PMID: 21738758
PDF is available here.
PDF is available here.
BMC Plant Biology (11) 2011
Abstract
We have identified and annotated the SERK genes in this species. Using degenerate PCR and database mining, eight more SERK-like genes have been identified and these have been shown to be expressed. The amplification and sequencing of several different PCR products from one of these genes is consiste...
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PMID: 21385462
PDF is available here.
PDF is available here.
Abstract
We experimentally assay-binding affinities for a variety of mutants and determine their relative free energy of binding, ΔΔG, to rank the importance of interface residues to binding. We then compare how accurately, precisely and reliably computational methods for calculating ΔΔG can replicate th...
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PMID: 20656696
PDF is available here.
PDF is available here.
Molecular Plant (4)1 2011
Abstract
We examined the effect of drought on the anther proteome of two rice genotypes: Moroberekan and IR64. Water was withheld for 3 d before heading (3DBH) in well watered controls for 5 d until the flag leaf relative water content (RWC) had declined to 45-50%. Plants were then re-watered and heading...
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PMID: 20643753
PDF is available here.
PDF is available here.
Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.] (Chapter 3) 2011
Abstract
Reaction conditions for a variety of endonucleases are detailed in this unit along with discussions of potential applications. Enzymes covered include BAL 31 nuclease, S1 nuclease, mung bean nuclease, micrococcal nuclease, and DNase I. A general discussion regarding the use of endonu...
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PMID: 21225639
PDF is available here.
PDF is available here.
Abstract
We show the accurate proteomic measurements in thylakoid membranes. The chlorophyll-containing membrane protein complexes were precipitated using chloroform/methanol solution. These complexes were separated using two-dimensional gel electrophoresis, and the resolved spots were exercised from the gel...
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PMID: 20960129
PDF is available here.
PDF is available here.
Abstract
We have described in this chapter the methods used in our laboratory for investigations of the import of nuclear-encoded proteins across the chloroplast envelope membranes, and for their further delivery into or across the thylakoid membrane by one of the four distinct pathways....
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PMID: 20960128
PDF is available here.
PDF is available here.
Abstract
We describe how to extract and quantify Rubisco activase content in leaf....
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PMID: 20960145
PDF is available here.
PDF is available here.
Abstract
Rubisco activase is a molecular chaperone that modulates the activation state of Rubisco by catalyzing the ATP-dependent removal of tightly-bound inhibitory sugar-phosphates from Rubisco's catalytic sites. This chapter reports methods developed for the purification of native and recombinant Rubisco...
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PMID: 20960143
PDF is available here.
PDF is available here.
Abstract
Ribulose-1,5-bisphosphate (RuBP) carboxylase/oxygenase (Rubisco) activase functions as a mechano-chemical motor protein using the energy from ATP hydrolysis to contort the structure of its target protein, Rubisco. This action modulates the activation state of Rubisco by removing tightly-bound inhibi...
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PMID: 20960144
PDF is available here.
PDF is available here.
Abstract
Redox-dependent thylakoid protein phosphorylation regulates both the short- and long-term acclimation of the photosynthetic apparatus to changes in environmental conditions. The major thylakoid phosphoproteins belong to photosystem II (D1, D2, CP43, PsbH) and its light-harvesting antenna (Lhcb1, Lhc...
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PMID: 20960130
PDF is available here.
PDF is available here.
Abstract
We evaluated the involvement of mitochondria and intracellular oxidative stress in M-to-Y differentiation. M-to-Y transition was delayed by the inhibition of mitochondrial Complexes III and IV or alternative oxidase (AOX) and was blocked by the association of AOX with Complexes III or IV inhibitors....
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PMID: 21183691
PDF is available here.
PDF is available here.
Abstract
Comparative targeted compositional analysis is currently an important element in the safety assessment of genetically modified plants. Profiling methods have been suggested as nontargeted tools to improve the detection of possible unintended effects. In this study, the capability of...
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PMID: 20968288
PDF is available here.
PDF is available here.
British Journal of Nutrition (104)12 2010
Abstract
We hypothesised that unheated phaseolin influences mucin flow and gene expression, and that phaseolin heat treatment reverses these effects. The hypothesis was tested using a control diet containing casein as the sole protein source and three other diets with casein being replaced by 33 and 67 % of...
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PMID: 20673379
PDF is available here.
PDF is available here.
Proteomics (10)24 2010
Abstract
We found several new salt-inducible proteins, whose expression has not been previously reported to be modulated by salt. A set of phosphoproteins in maize was detected but only ten proteins were phosphorylated and six proteins were dephosphorylated after the application of 25 mM NaCl for 1 h. So...
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PMID: 21136597
PDF is available here.
PDF is available here.
Abstract
Our recent report documented that the rice germin-like protein1 (OsGLP1), being a cell wall-associated protein involves in disease resistance in rice and possesses superoxide dismutase (SOD) activity as recognized by heterologous expression in tobacco. In the present study, the transgenic tobacco pl...
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PMID: 20971065
PDF is available here.
PDF is available here.
Abstract
Employing the utility of the native chemical ligation, site-specific attachment of an ultrastable perylene dye to a derivative of the major light-harvesting complex (LHCII) was demonstrated. Biochemical analysis of the conjugate indicated that the structure and function of LHCII remain largely unaff...
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PMID: 20820669
PDF is available here.
PDF is available here.
Journal of Proteomics (73)12 2010
Abstract
We find that the only fining agent used is bovine casein, just like in white wines. Although the typical levels of casein found range between 45 to 85μg/L, in one case as little as 3.8μg/L of casein could be detected, an extremely high level of sensitivity, close to our lower detection limit of 1...
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PMID: 20813213
PDF is available here.
PDF is available here.
Molecular Plant (3)6 2010
Abstract
There is increasing evidence that pathogens do not only elicit direct defense responses, but also cause pronounced changes in primary carbohydrate metabolism. Cell-wall-bound invertases belong to the key regulators of carbohydrate partitioning and source-sink relations. Whereas studi...
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PMID: 20833735
PDF is available here.
PDF is available here.
Abstract
Paramutation refers to the process by which homologous DNA sequences communicate in trans to establish meiotically heritable expression states. Although mechanisms are unknown, current data are consistent with the hypothesis that the establishment and heritable transmission of specific chromatin sta...
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PMID: 21030647
PDF is available here.
PDF is available here.
Journal of Plant Physiology (167)16 2010
Abstract
I and II was performed with the aim to investigate salt-induced variations in photosystem composition. Net CO2 assimilation rate (Pn) increased under salinity up to 3 weeks of treatment then decreased to reach the value of 0mM-treated plants at the end of the experiment. For stomatal conductance (gs...
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PMID: 20619928
PDF is available here.
PDF is available here.
Abstract
We report a transporter, Nrat1 (Nramp aluminum transporter 1), specific for trivalent Al ion in rice. Nrat1 belongs to the Nramp (natural resistance-associated macrophage protein) family, but shares a low similarity with other Nramp members. When expressed in yeast, Nrat1 transports trivalent Al ion...
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PMID: 20937890
PDF is available here.
PDF is available here.
Abstract
We describe the positional cloning of two soybean genes Rj2 and Rfg1 that restrict nodulation with specific strains of Bradyrhizobium japonicum and Sinorhizobium fredii, respectively. We show that Rj2 and Rfg1 are allelic genes encoding a member of the Toll-interleukin receptor/nucleotide-binding si...
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PMID: 20937853
PDF is available here.
PDF is available here.
Abstract
We report that both SAR Deficient 1 (SARD1) and CBP60g are key regulators for ICS1 induction and SA synthesis. Whereas knocking out SARD1 compromises basal resistance and SAR, overexpression of SARD1 constitutively activates defense responses. In the sard1-1 cbp60g-1 double mutant, pathogen-induced...
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PMID: 20921422
PDF is available here.
PDF is available here.
Abstract
We have cloned and expressed in Escherichia coli two dehydrins from this plant, denoted TsDHN-1 (acidic) and TsDHN-2 (basic). Here, we show using transmission-Fourier transform infrared (FTIR) spectroscopy that ordered secondary structure is induced and stabilized in these proteins by association wi...
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PMID: 20921991
PDF is available here.
PDF is available here.
Abstract
In a previous work, the immunolocation of the chickpea XTH1 (xyloglucan endotransglucosylase/hydrolase 1) protein in the cell walls of epicotyls, radicles, and stems was studied, and a role for this protein in the elongation of vascular cells was suggested. In the present work, the presence and the...
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PMID: 20643805
PDF is available here.
PDF is available here.
Abstract
I small heat shock protein (sHSP-CI) genes were found to be selectively induced by L-azetidine-2-carboxylic acid (AZC) on chromosome 3 but not chromosome 1. Here it is shown that a novel cis-responsive element contributed to the differential regulation. By serial deletion and computational analysis,...
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PMID: 20643810
PDF is available here.
PDF is available here.
Abstract
We report the isolation and analysis of CCD4 genomic DNA regions in Crocus sativus. Different CCD4 alleles have been identified: CsCCD4a which is found with and without an intron and CsCCD4b that showed the presence of a unique intron. The presence of different CCD4 alleles was also observed in othe...
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PMID: 20633636
PDF is available here.
PDF is available here.
Abstract
We have identified in silico a set of miRNAs that control helicase gene expression by regulating its mRNA stability and translation in rice. Our analysis revealed that several rice helicases have distinct miRNA specificities. Such analyses will be a prerequisite to refining our understanding of targ...
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PMID: 20953156
PDF is available here.
PDF is available here.
Abstract
An efficient protein extraction method is crucial to ensure successful separation by two-dimensional electrophoresis (2-DE) for recalcitrant plant species, in particular for grapevine (Vitis vinifera L.). Trichloroacetic acid-acetone (TCA-acetone) and phenol extraction methods were e...
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PMID: 20883445
PDF is available here.
PDF is available here.
Abstract
We used integrated proteomic and cytological approaches to probe the interconnections in rice (Oryza sativa) endosperm at the storage phase from 12 to 18 days after flowering (DAF). Starch granule packaging was completed first in the inner part of endosperm at 15 DAF and spread to almost the entire...
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PMID: 20712379
PDF is available here.
PDF is available here.
Food and Chemical Toxicology (48)10 2010
Abstract
We sought to assess the allergenic potential of red gram by identifying and characterizing the responsible proteins. Immunoblotting was performed to detect IgE binding proteins. Identities of these proteins were confirmed by mass spectrometry. To evaluate allergenic potential, BALB/c mice were sensi...
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PMID: 20600518
PDF is available here.
PDF is available here.
Abstract
Panax ginseng C. A. Meyer is a perennial herb from the Araliaceae family. Traditionally used as a medicinal plant in Oriental medicine for more than thousand years. Ginsenosides are the major therapeutic components in ginseng roots. Roots of the ginseng plant have more commercial value and based on...
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PMID: 19943115
PDF is available here.
PDF is available here.