Abstract
We have identified in silico a set of miRNAs that control helicase gene expression by regulating its mRNA stability and translation in rice. Our analysis revealed that several rice helicases have distinct miRNA specificities. Such analyses will be a prerequisite to refining our understanding of targ...
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PMID: 20953156
PDF is available here.
Abstract
An efficient protein extraction method is crucial to ensure successful separation by two-dimensional electrophoresis (2-DE) for recalcitrant plant species, in particular for grapevine (Vitis vinifera L.). Trichloroacetic acid-acetone (TCA-acetone) and phenol extraction methods were e...
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PMID: 20883445
PDF is available here.
Abstract
We used integrated proteomic and cytological approaches to probe the interconnections in rice (Oryza sativa) endosperm at the storage phase from 12 to 18 days after flowering (DAF). Starch granule packaging was completed first in the inner part of endosperm at 15 DAF and spread to almost the entire...
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PMID: 20712379
PDF is available here.
Abstract
We investigated the physiological and molecular responses of rice to inoculation with the AM fungus Glomus intraradices, focusing on the relevance of the plant defense response during the symbiotic mycorrhizal interaction. • Defense gene expression analysis and proteomic approaches were used. The...
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PMID: 20659300
PDF is available here.
Abstract
Our results suggest an ancestral role of WOX in seed plant embryo development, and strengthen the proposed connection between PAT, PIN-FORMED (PIN) and WOX in the regulation of embryo patterning in seed plants....
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PMID: 20561212
PDF is available here.
Abstract
The extent to which vacuolar sugar transport activity affects molecular, cellular, and developmental processes in Arabidopsis (Arabidopsis thaliana) is unknown. Electrophysiological analysis revealed that overexpression of the tonoplast monosaccharide transporter TMT1 in a tmt1-2::tDNA mutant led to...
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PMID: 20709831
PDF is available here.
Abstract
Production of recombinant Oryza sativa non-symbiotic hemoglobin 1 (OsHb1) by Escherichia coli was maximized in shake-flask cultures in media containing tryptone, yeast extract, sodium chloride and byproduct glycerol from biodiesel production. Response surface methodology (RSM) and artificial neural...
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PMID: 20578289
PDF is available here.
Abstract
Legumes (Fabaceae) are unique in their ability to enter into an elaborate symbiosis with nitrogen-fixing rhizobial bacteria. Rhizobia-legume (RL) symbiosis represents one of the most productive nitrogen-fixing systems and effectively renders the host plants to be more or less indepen...
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PMID: 20621211
PDF is available here.
Abstract
We implemented differential centrifugation and then shotgun proteomics based on nanoliquid chromatography/mass spectrometry (nano-LC-MS/MS) for peptide separation and analysis. A unigene database available for E. californica was translated and utilized for protein identification. Approximately 646 p...
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PMID: 20690678
PDF is available here.
Abstract
Ozone (O3), a potent air pollutant and a significant greenhouse gas, has been recognized as a major component of global climate change. However, current increasing trends in its background level are projecting a more severe threat to natural and cultivated plants in the near future....
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PMID: 20701290
PDF is available here.
Abstract
Component resolved diagnostics introduces new possibilities regarding diagnosis of allergic diseases and individualized, allergen-specific treatment. Furthermore, refinement of IgE-based testing may help elucidate the correlation or lack of correlation between allergenic sensitization and allergic d...
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PMID: 20628838
PDF is available here.
Abstract
We have studied an embryo-specific, zinc-binding metallothionein (E(C)) from wheat by nuclear magnetic resonance, electrospray mass spectrometry, site-directed mutagenesis, and molecular modelling. Wheat E(C) exploits differences in affinities of Cys(4) and Cys(2)His(2) sites for Cd(2+) and Zn(2+) t...
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PMID: 20467686
PDF is available here.
Abstract
We previously demonstrated that jack bean urease (JBU) has antidiuretic effects on Rhodnius prolixus Malpighian tubules, decreasing the serotonin-stimulated secretion of fluid. Now, we evaluate the toxicity of the intact JBU and its effect on R. prolixus anterior midgut, to further elucidate the mec...
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PMID: 20223243
PDF is available here.
Abstract
We therefore conclude that urea interacts favorably with the whole protein surface and that all parts of the protein are important in urea-induced denaturation....
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PMID: 20563326
PDF is available here.
Abstract
We isolated the full-length cDNA encoding a Triticum aestivum CP gene, designated TaCP, from wheat by the rapid amplification of cDNA ends (RACE) method. Sequence analysis revealed that TaCP contains an open reading frame encoding a protein of 362 amino acids, which is 96% identical to barley cystei...
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PMID: 20826947
PDF is available here.
Abstract
We generated a whole array of 35S-OsWRKY transgenic Arabidopsis. In this study, we report that 35S-OsWRKY72 transgenic Arabidopsis, whose seed germination was retarded under normal conditions, emerged more sensitive to mannitol, NaCl, ABA stresses and sugar starvation than vector plants. Meanwhile,...
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PMID: 20826955
PDF is available here.
Abstract
We have used neem leaf glycoprotein (NLGP), a novel immunomodulator reported from our laboratory. NLGP upregulated CCR5 expression, as evidenced from studies on MO/Mphi of peripheral blood from HNSCC patients as well as healthy individuals. Expression of RANTES, MIP-1alpha and MIP-1beta was also upr...
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PMID: 20622890
PDF is available here.
Abstract
We have obtained a proteomic reference map of B. japonicum strain CPAC 15 (=SEMIA 5079) - used in commercial inoculants for application to soybean crops in Brazil - grown under in vitro conditions. CPAC 15 belongs to the same serogroup as strain USDA 123, and both are known as the soybean bradyrhizo...
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PMID: 20806226
PDF is available here.
Abstract
We studied the physiological and proteomic changes of C. spinarum in response to 42 degrees C heat stress treatment in combination with drought stress. Dynamic changes in the leaf proteome were analyzed at four time points during the stress treatment and recovery stages. Approximately, 650 protein s...
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PMID: 20661954
PDF is available here.
Abstract
To explore the method for improving the aging resistance of seeds and seedlings of Perilla frutescens through study on seed germination and physiological characteristics of P. frutescens seedlings.
Several physiological indexes of P. frutescens seeds treated by different concentrations of ZnSO4 and...
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PMID: 21141480
PDF is available here.