Abstract
We found that the degradation of aqueous based silk is much faster than HFIP based silk scaffolds. Also, HFIP based silk scaffolds supported the soft dental pulp formation better than the aqueous based silk scaffolds. No distinct hard tissue regeneration was found in any of the implants, with or wit...
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PMID: 21484985
PDF is available here.
Abstract
Tendon disorders are common clinical conditions. Tendon tissue engineering provides a new approach for tendon repair by integrating engineered substitutes with their native counterparts. Silk is considered to be a promising candidate for tendon engineering because of its biological a...
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PMID: 21555843
PDF is available here.
Abstract
We aimed to apply this concept to a three-dimensional full silk fibroin (SF) hybrid scaffold system, which comprised of knitted SF and aligned SF electrospun fibers (SFEFs), for ligament tissue engineering applications. Specifically, knitted SF, which contributed to the mechanical robustness of the...
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PMID: 21501090
PDF is available here.
Abstract
Silk fibroin protein is biodegradable and biocompatible, exhibiting excellent mechanical properties for various biomedical applications. However, porous three-dimensional (3-D) silk fibroin scaffolds, or silk sponges, usually fall short in matching the initial mechanical requirements...
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PMID: 20656075
PDF is available here.
Abstract
Our findings show that fibroin microparticles may present an interesting option for future clinical applications in the bone tissue engineering field, and therefore, further studies have been planned....
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PMID: 19958078
PDF is available here.
Abstract
We summarize recent progress in research employing recombinantly produced engineered spider silk proteins with a focus on the fundamentals of silk protein processing. We highlight recombinant spider silk films and particles as morphologies that represent model systems with adjustable material proper...
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PMID: 20602494
PDF is available here.
Abstract
Spiders and silkworms generate silk protein fibers that embody strength and beauty. Orb webs are fascinating feats of bioengineering in nature, displaying magnificent architectures while providing essential survival utility for spiders. The unusual combination of high strength and extensibility is a...
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PMID: 20671180
PDF is available here.
Abstract
We present the experimental results for the two-dimensional backscattering Mueller matrix of a scattering medium containing polystyrene microspheres and silk fibers and simulate the same Mueller matrix using a polarization-sensitive Monte Carlo program with both layered and homogeneous sphere-cylind...
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PMID: 20634817
PDF is available here.
Abstract
We analyzed the tissue-specific expression patterns in the silk gland, fat body, and midgut five days of fifth instar larvae during the development of B. mori. To verify the tissue-specific expression, analysis was conducted using quantitative Real-time RT-PCR and the highly expressed endogenous Act...
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PMID: 20663409
PDF is available here.
Abstract
We have investigated the effects of nanoroughness assemblies of silk fibroin protein membranes and RGD sequences fabricated from two different silk fibroin sources, that is, mulberry (Bombyx mori) and nonmulberry (Antheraea mylitta), on cytoskeletal organization, proliferation, and viability using p...
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PMID: 20214452
PDF is available here.
Abstract
We investigated some factors that might be responsible for this preference using similar cardboard refugia. When the two Loxosceles species were given choices between refugia previously occupied by their own and by the congeneric species, neither showed a species-specific preference; however, each c...
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PMID: 20568627
PDF is available here.
Dae-Hyeong DH Kim,
Jonathan J Viventi,
Jason J JJ Amsden,
Jianliang J Xiao,
Leif L Vigeland,
Yun-Soung YS Kim,
Justin A JA Blanco,
Bruce B Panilaitis,
Eric S ES Frechette,
Diego D Contreras,
David L DL Kaplan,
Fiorenzo G FG Omenetto,
Yonggang Y Huang,
Keh-Chih KC Hwang,
Mitchell R MR Zakin,
Brian B Litt and
John A JA Rogers
Abstract
Electronics that are capable of intimate, non-invasive integration with the soft, curvilinear surfaces of biological tissues offer important opportunities for diagnosing and treating disease and for improving brain/machine interfaces. This article describes a material strategy for a type of bio-inter...
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PMID: 20400953
PDF is available here.
Abstract
Stress urinary incontinence remains a worldwide problem affecting patients of all ages. Implantation of suburethral sling is the cornerstone treatment. Current slings have inherent disadvantages. This study aims to develop a tissue engineered sling with bone marrow derived mesenchymal stem cell seed...
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PMID: 20303586
PDF is available here.
Abstract
We reveal the sophisticated and hierarchical structure of honeybee combs and measure the elastic properties of fresh and old natural honeycombs at different scales by optical microscope, environmental scanning electron microscope, nano/microindentation, and by tension and shear tests. We demonstrate...
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PMID: 20439765
PDF is available here.
Abstract
There was no significant difference between groups at 6 weeks after operation. In the micro-CT results, however, tissue mineral content in the experimental group at 12 weeks after operation was 132.09 +/- 4.41 and that in the control group was 126.42 +/- 6.62 (P = .011). Tissue mineral density in th...
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PMID: 20149696
PDF is available here.
Abstract
We elucidate the effects of such stimuli on the in vitro aggregation of engineered and recombinantly produced major ampullate silk-like proteins (focusing on structure-function relationships with respect to their primary structures), and discuss their relevance to the storage and assembly of spider...
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PMID: 20045467
PDF is available here.
Abstract
We engineered constructs mimicking distinct domains of FLAG (eFLAG) and produced them recombinantly to analyze the structure-function relationship of FLAG domains and assembly properties of FLAG. While in solution the small carboxy-terminal domain is structured, domains from the repetitive core regi...
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PMID: 20045468
PDF is available here.
Abstract
We set out to investigate what effects blending silk with MBG had on the physiochemical, drug-delivery and biological properties of MBG scaffolds with a view to bone tissue engineering applications. Transmission electron microscopy (TEM), scanning electron microscopy (SEM) and Fourier transform infr...
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PMID: 20122721
PDF is available here.
Abstract
Complete amino acid sequences of the four major proteins (Vssilk 1-4) of silk (hornet silk) obtained from yellow hornet ( Vespa simillima , Vespinae, Vespidae) cocoons have been determined. The native structure of the hornet silk (HS), in which Vssilk 1-4 have an alpha-helix domain with coiled-coil...
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PMID: 20178379
PDF is available here.
Abstract
One conversion to beating heart surgery with cardiopulmonary bypass support occurred in each study group. No patient in the LPS group was converted to DPS technique. The use of LPSs allowed a number of distal anastomoses somewhat higher than the DPS technique (4.1+/-1.1 vs. 3.7+/-1.1, P=0.02). Posto...
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PMID: 20354498
PDF is available here.
Abstract
Adverse environmental conditions can be evaded, tolerated or modified in order for an organism to survive. During their development, some insect larvae spin cocoons which, in addition to protecting their occupants against predators, modify microclimatic conditions, thus facilitating thermoregulation...
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PMID: 20204318
PDF is available here.
Abstract
We report an in vitro assessment of the effect of nonmodified, degummed silks on cells. We describe the effects of degummed silk fibers as well as extracted sericin on cell metabolism and proliferation. Endothelial cells directly exposed to native degummed Bombyx mori and Antheraea pernyi silks show...
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PMID: 19353564
PDF is available here.
Abstract
We summarize the current research related to silk being used as a material for cell culture and tissue engineering, particularly focusing on cell-surface adherence, mechanical and textural properties, toxicity, immunogenicity and biodegradability....
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PMID: 20222871
PDF is available here.
Abstract
We can learn from these past failures, and potentially develop a bioresorbable scaffold for ACL reconstruction, which will provide immediate stability, promote and direct tissue in growth, and degrade at an appropriate rate, without harmful wear debris. We have developed a modified silk scaffold, wh...
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PMID: 20160629
PDF is available here.
Abstract
We propose a simple model to describe spider orb webs. The model has a formal analytical solution when no thread elements are broken. When the radial threads are sufficiently strong compared to the spiral threads, the model is free of stress concentrations even when a few spiral threads are broken....
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PMID: 20366686
PDF is available here.
Abstract
Caries and periodontal diseases among the workers of silk factory, where the professional harmfulness were: discomfort of the microclimate, the intensive noise, psychological loads, bound with the monotonic of labor process, enforced working posture, high bacterial dirty of the rooms, were detected...
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PMID: 20559231
PDF is available here.
Abstract
We have previously demonstrated that porous silk scaffolds can support AF cell attachment and extracellular matrix accumulation; however, tissue infiltration and matrix accumulation was not optimal. The purpose of this study was to determine whether the dynamic culture of AF cells seeded into larger...
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PMID: 19165797
PDF is available here.
Abstract
We hypothesized that Smsp-72k is an adhesive/cement protein that increases the adhesiveness of the silk fiber of S. marmorata. The hydroxyl groups of Smsp-72k might form a link with the heavy chain fibroin of S. marmorata, removing the weak boundary-water layer and allowing the spreading of the silk...
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PMID: 20057124
PDF is available here.
Abstract
The purpose of this study was to prepare and characterize a three-dimensional scaffold consisting of porous nonwoven silk fibroin net/nano-apatite composite. The silk fibroin net was mineralized with apatite by alternative soaking in calcium and phosphate solutions. The scaffold exhibited a porous m...
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PMID: 19148924
PDF is available here.
Abstract
We demonstrate controllable structural color based on periodic nanopatterned 2D lattices in pure protein films of silk fibroin. We show here periodic lattices in silk fibroin films with feature sizes of hundreds of nanometers that exhibit different colors as a function of varying lattice spacing. Fu...
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PMID: 19997366
PDF is available here.
Abstract
Restoration of a three-dimensional shape with soft tissue augmentation is a challenge for surgical reconstruction and esthetic improvement of intraoral mucosa and perioral skin tissues. A connective tissue graft or free gingival graft, classically used for such indications, requires a donor site, wh...
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PMID: 19905955
PDF is available here.
Abstract
We report an Antheraea assama silk-based non-woven fibroin scaffold for applications in TERM. The novel three-dimensional scaffold is highly interconnected and porous, with a pore size of 150 microm, porosity of 90% and water uptake capacity of 85%. FTIR revealed a typical beta-sheet structure of fi...
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PMID: 19670334
PDF is available here.
Abstract
Silk fibroin (SF) has been one of promising resources of biotechnology and biomedical materials due to its unique properties. Here, different sizes of porous tubular scaffolds were fabricated from a SF aqueous solution with the addition of poly(ethylene glycol diglycidyl ether) (PGDE). The scaffolds...
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PMID: 19793450
PDF is available here.
Abstract
We present diameter measurements, tensile tests, and protein secondary structural analyses of silks spun by several embiid species. Despite their diverse habitats and phylogenetic relationships, these species have remarkably similar silk diameters and ultimate stress values. Yet, ultimate strain, Yo...
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PMID: 19572641
PDF is available here.
Abstract
I and silk II, at very low LiBr concentrations, showed the best spinning process stability and physical properties. This underpins the prediction that the enhanced control over structure and phase behavior using the osmotic stress method helps improve the physical properties of wet-spun regenerated...
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PMID: 19572633
PDF is available here.
Abstract
In this study, a tubular scaffold composed of polylactide fibers (outside layer) and silk fibroin-gelatin fibers (inner layer) was fabricated successfully by electrospinning. Morphological, biomechanical, and dissolvable properties of the composite scaffolds were examined, in particu...
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PMID: 19722559
PDF is available here.
Abstract
We aim to give an overview of the design principles of such silk-inspired polymers/proteins, their processing into various materials morphologies, their mechanical and biological properties, and, finally, their technical and biomedical applications....
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PMID: 19614574
PDF is available here.
Abstract
SEM observation: the scaffold in two groups had interconnected porous network structure; the fiber diameter in the experimental and the control group was (141 +/- 9) nm and (205 +/- 11) nm, respectively; the pores in the scaffold were interconnected; the porosity was 87.4% +/- 1.1% and 85.3% +/- 1.3...
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PMID: 19728623
PDF is available here.
Abstract
We could demonstrate in vivo that the implantation of SFCS scaffolds resulted in only slight inflammation. Keeping the good histocompatibility and combining the advantages of both fibroin and chitosan, the SFCS scaffold could be a prominent candidate for soft tissue engineering, for example, in the...
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PMID: 19671956
PDF is available here.
Abstract
We could distinguish between proton chemical shift effects arising from intermolecular interactions and those from intramolecular interactions by combining observation of the solid state 1H NMR chemical shift and empirical chemical shift calculation....
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PMID: 19569641
PDF is available here.
Abstract
Tissue engineering is an alternative approach for the preparation of small-diameter (<6mm) vascular grafts due to the potential to control thrombosis, anastomotic cellular hyperplasia and matrix production. This control also requires the maintenance of graft patency in vivo, appropriate mechanical p...
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PMID: 19232717
PDF is available here.
Abstract
These results suggest that both the thickness and plasticity of the indicating media affect neuromuscular function during occlusion....
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PMID: 19639919
PDF is available here.
Abstract
The study aimed to investigate and compare some characteristics of Bombyx mori and Eri (Philosamia ricini) silks in different forms; with and without sericin. The protein contents were measured and find out the composition of the silk fibroin and sericin proteins by Lowry method. The secondary struc...
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PMID: 19803122
PDF is available here.
Abstract
The cytotoxicity of pNSR16/PVA scaffold was in grade 0. Scanning electron microscope observation: the cells covered the surface of the scaffold and were arranged in a directional manner 4 days after co-culture. HE staining: the cells adhered to and grew on the surface of scaffold, and migrated into...
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PMID: 19594027
PDF is available here.
Abstract
Proteins are ubiquitous biopolymers that adopt distinct three-dimensional structures and fulfil a multitude of elementary functions in organisms. Recent systematic studies in molecular biology and biotechnology have improved the understanding of basic functional and architectural principles of prote...
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PMID: 19376768
PDF is available here.
Abstract
We consider the attributes of a successful engineered material, acknowledging the contributions of composition and processing to properties and performance. We recognize the potential for relevant lessons to be learned from nature, at the same time conceding both the limitations of such lessons and...
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PMID: 19324724
PDF is available here.
Abstract
We utilized a proteomic approach to investigate the fifth instar prothoracic gland during the growth and development of the silkworm, Bombyx mori L. The two-dimensional electrophoresis results showed that the majority of proteins were acidic proteins, especially concentrated in the area of 25-65 kDa...
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PMID: 18715222
PDF is available here.
Abstract
We review the pertinent literature, focusing on potential camouflage and mimicry. Webs are often difficult to detect in a heterogeneous visual environment. Static and dynamic web distortions are used to escape visual detection by prey, although particular silk may also attract prey. Recent work usin...
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PMID: 18990672
PDF is available here.
Abstract
Biomaterials for corneal tissue engineering must demonstrate several critical features for potential utility in vivo, including transparency, mechanical integrity, biocompatibility and slow biodegradation. Silk film biomaterials were designed and characterized to meet these functional requirements....
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PMID: 19059642
PDF is available here.
Vladimir G Bogush,
Olga S Sokolova,
Lyubov I Davydova,
Dmitri V Klinov,
Konstantin V Sidoruk,
Natalya G Esipova,
Tatyana V Neretina,
Igor A Orchanskyi,
Vsevolod Yu Makeev,
Vladimir G Tumanyan,
Konstantin V Shaitan,
Vladimir G Debabov and
Mikhail P Kirpichnikov
Abstract
We modeled the complete process of the spider silk assembly using two new recombinant analogs of spidroins 1 and 2. The artificial genes sequence of the hydrophobic core regions of spidroin 1 and 2 have been designed using computer analysis of existing databases and mathematical modeling. Both prote...
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PMID: 18839314
PDF is available here.
Abstract
The design, construction, and preliminary characterization of a novel family of spider silk-like block copolymers are described. The design was based on the assembly of individual spider silk modules, in particular, polyalanine (A) and glycine-rich (B) blocks, that display different...
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PMID: 19128057
PDF is available here.
Abstract
The design, construction, and preliminary characterization of a novel family of spider silk-like block copolymers are described. The design was based on the assembly of individual spider silk modules, in particular, polyalanine (A) and glycine-rich (B) blocks, that display different...
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PMID: 19128057
PDF is available here.
Abstract
The design, construction, and preliminary characterization of a novel family of spider silk-like block copolymers are described. The design was based on the assembly of individual spider silk modules, in particular, polyalanine (A) and glycine-rich (B) blocks, that display different...
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PMID: 19128057
PDF is available here.
Abstract
The design, construction, and preliminary characterization of a novel family of spider silk-like block copolymers are described. The design was based on the assembly of individual spider silk modules, in particular, polyalanine (A) and glycine-rich (B) blocks, that display different...
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PMID: 19128057
PDF is available here.