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Antennapedia Homeodomain Protein (0):
Recent article
Antennapedia Homeodomain Protein
Abstract
We have reported the results of 20ns MD simulation of this complex with the presence of explicit water and also the 20ns MD simulation of the protein and the DNA separately in explicit water. The results show that the complex remains stable during the last 8ns of the simulation. The part of the prot...
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PMID: 19916566
PDF is available here.
PDF is available here.
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Abstract
We have studied whether the PTD-peptide penetratin, derived from a Drosophila Antennapedia homeodomain protein, is capable of crossing the BBB in Drosophila while carrying a cargo into the fly brain. An initial in vivo experiment in Drosophila showed that abdominal injection of biotin-tagged penetra...
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PMID: 19351032
PDF is available here.
PDF is available here.
Abstract
We have proposed that the eye loss phenotype is a consequence of a negative posttranslational control mechanism because of direct protein-protein interactions between ANTP and Eyeless (EY). In the present work, we analyzed the effect of various ANTP homeodomain mutations for their interaction with E...
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PMID: 18755899
PDF is available here.
PDF is available here.
Development (135)9 2008
Abstract
We investigated the importance of the Antennapedia (Antp) YPWM motif for homeotic transformations in adult Drosophila. By ectopic overexpression, the head structures of the fly can be transformed into structures of the second thoracic segment, such as antenna into second leg, head capsule into thora...
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PMID: 18367556
PDF is available here.
PDF is available here.
FASEB Journal (22)4 2008
Abstract
We constructed a single-chain variable fragment miniantibody (G11-scFv) directed toward the transactivation domain of c-Myc, which is fused with the internalization domain Int of Antennapedia at its carboxyl terminus (a cargo-carrier construct). In ELISA experiments, an EC(50) for binding saturation...
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PMID: 18048579
PDF is available here.
PDF is available here.
Abstract
The relative order of the transduction efficacy of these PTDs conjugated to fluorescein was Rev>Antp>Tat>VP22, independent of cell type (HeLa, HaCaT, A431, Jurkat, MOLT-4 and HL60 cells). Antp produced significant toxicity in HeLa and Jurkat cells, and Rev produced significant toxicity in Jurkat cel...
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PMID: 18223668
PDF is available here.
PDF is available here.
Abstract
NT4p53(N15)Ant significantly inhibited the proliferation of HepG2 cells. The survival rates of HepG2 cells in NT4p53(N15)Ant group were 36.67% at 48 h, 20.47% at 72 h and 17.82% at 96 h after infection, which were significantly lower than those in Ad-GFP group (P<0.05). Distinct cell membrane disrup...
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PMID: 18279610
PDF is available here.
PDF is available here.
Abstract
In this study, we report a method for the fast and efficient retrieval of sequences belonging to the ANTP (HOXL and NKL) and PRD classes. Furthermore, we look for diagnostic amino acid residues that can be used to distinguish HOXL, NKL and PRD genes. CONCLUSION: The reported protein features will fa...
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PMID: 18620554
PDF is available here.
PDF is available here.
Abstract
We identify in the Drosophila Ultrabithorax (Ubx) protein a short motif responsible for an alternative mode of Exd recruitment. Together with previous reports, this finding highlights that the Hox protein Ubx has multiple ways to interact with the Exd cofactor and suggests that flexibility in Hox-PB...
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PMID: 17942685
PDF is available here.
PDF is available here.
Abstract
We examined whether cell-permeable peptides derived from the BH3 domains of proapoptotic Bax, Bad, or Bak could be used to target Bcl-XL and/or Bcl-2 in HNSCC cells, and induce apoptotic death in these cells. To render the peptides cell permeable, Antennapedia (Ant) or polyarginine (R8) peptide tran...
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PMID: 17971900
PDF is available here.
PDF is available here.
Abstract
Brain capillary endothelial cells (BCECs) have been considered as one of the primary targets for cerebral gene therapy. However, the cells, well-known for their poor function of endocytosis, are difficult to be transfected by general non-viral vectors. The aim of this study was to enhance the effici...
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PMID: 17447157
PDF is available here.
PDF is available here.
Developmental Dynamics (236)8 2007
Abstract
We present a detailed analysis of Asx's role in antennal development. We show that loss of Asx in the antennal disc causes a complex phenotype, which consists of distal antenna-to-leg transformations and outgrowth of ectopic leg-like appendages from the Dpp-expressing domain of the disc. Our analyse...
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PMID: 17654717
PDF is available here.
PDF is available here.
Journal of Genetics (86)2 2007
Abstract
These results indicate that there may be additional molecules involved in antenna/leg identity and that spatial, temporal and dosage relationships are more subtle than suspected and must be part of a robust understanding of molecular network behaviour involved in determining appendage identity in Dr...
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PMID: 17968139
PDF is available here.
PDF is available here.
Current Biology (17)8 2007
Abstract
We show that Hox genes' origin occurred after the divergence of sponge and eumetazoan lineages and occurred concomitantly with a major evolutionary transition in animal body-plan complexity. By using whole genome information from the demosponge Amphimedon queenslandica, we provide the first conclusi...
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PMID: 17379523
PDF is available here.
PDF is available here.
Abstract
We show that the PSC is specified early in the embryo by the homeotic gene Antennapedia (Antp) and expresses the signalling molecule Hedgehog. In the absence of the PSC or the Hedgehog signal, the precursor population of the medullary zone is lost because cells differentiate prematurely. We conclude...
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PMID: 17361183
PDF is available here.
PDF is available here.
Development (134)6 2007
Abstract
We investigate the role of PcG genes in postembryonic development of the Drosophila CNS. We use mosaic-based MARCM techniques to analyze the role of these genes in the persistent larval neuroblasts and progeny of the central brain and thoracic ganglia. We find that proliferation in postembryonic neu...
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PMID: 17287254
PDF is available here.
PDF is available here.
Developmental Biology (302)2 2007
Abstract
We identify the antennal disc enhancer of ss and then use enhancer-lacZ reporters to work out how ss antennal expression is regulated. The antennal determinants Distal-less (Dll) and homothorax (hth) are key activators of the antennal enhancer. Dll is required continuously and, when present at eleva...
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PMID: 17084833
PDF is available here.
PDF is available here.
Voprosy onkologii (53)4 2007
Abstract
The study is concerned with proapoptotic properties of chimera peptides which incorporate sequences of inhibitors of cyclin kinases p161NK4a and p21CIP/WAF1 as well as internalized sequences (Antp and tat). Sequences of the p16 type appeared to be more cytotoxic than the p21 one. Cytotoxic effect pr...
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PMID: 17969409
PDF is available here.
PDF is available here.
Abstract
I were employed to investigate the stability. The results indicated that the gene products of DNA/CH nanoparticles and DNA/Lipofectamine 2,000 complexes whether modified or unmodified with Antp could not be observed within BCECs. The DNA/PEI nanoparticles and DNA/PAMAM nanoparticles could be interna...
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PMID: 16946530
PDF is available here.
PDF is available here.
Abstract
We found that a peptidomimetic of apoE protein, COG133, substantially reduces the clinical symptoms of EAE and promotes remission from the disability when administered before or after onset of disease. Most notably, fusion of COG133 to a protein transduction domain creates COG112, a modified apoE-mi...
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PMID: 16740622
PDF is available here.
PDF is available here.
Journal of Molecular Biology (361)4 2006
Abstract
We have investigated the conformation and dynamics of the N-terminal 101 amino acid residues of Brk in the absence and in the presence of cognate DNA by solution NMR spectroscopy. In the absence of DNA, Brk is unfolded and highly flexible throughout the entire backbone. Addition of cognate DNA induc...
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PMID: 16876822
PDF is available here.
PDF is available here.
Abstract
The results suggest that, when coupled with antennapedia, the antisense oligonucleotide against Bcl-XL could be a good therapeutic tool for radiosensitization of pancreatic cancer....
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PMID: 16904964
PDF is available here.
PDF is available here.
Abstract
Our data add to the number of published poriferan homeobox sequences and provide independent confirmation of the current Demospongiae phylogenies....
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PMID: 16786434
PDF is available here.
PDF is available here.
BioTechniques (40)5 2006
Abstract
Abstract
We have compared the cellular uptake efficiencies and interaction properties in a membrane-mimicking environment of four peptides corresponding to the third helix sequence of Antennapedia, Engrailed-2, HoxA-13, and Knotted-1. NMR studies revealed that these peptides adopt helical conformations in SD...
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PMID: 16445283
PDF is available here.
PDF is available here.
Abstract
Homeodomains are helix-turn-helix type DNA-binding domains that exhibit sequence-specific DNA binding by insertion of their "recognition" alpha helices into the major groove and a short N-terminal arm into the adjacent minor groove without inducing substantial distortion of the DNA....
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PMID: 16388589
PDF is available here.
PDF is available here.
Nippon rinsho. Japanese journal of clinical medicine (64 Suppl 1) 2006
Abstract
Abstract
Expression of trunk Hox genes in the centipede Strigamia maritima: sense and anti-sense transcripts.
Abstract
We report the coding sequence and embryonic expression of the four trunk Hox genes Antennapedia (Antp), Ultrabithorax (Ubx), abdominal-A (abd-A), and Abdominal-B (Abd-B) in the geophilomorph centipede Strigamia maritima. In geophilomorph centipedes, all leg-bearing segments (LBS) are generated durin...
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PMID: 16686636
PDF is available here.
PDF is available here.
Abstract
We found that the DNA-binding domain (homeodomain) of Antennapedia, a homeoprotein transcription factor, was internalized by live cells gaining access to their cytoplasm and nuclei. It was soon revealed that internalization is due to the third helix of the homeodomain, composed of sixteen amino acid...
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PMID: 17417134
PDF is available here.
PDF is available here.
Abstract
We report the abundant expression of transcriptional readthrough products of two adjacent Hox genes, Ubx, and Antp, in five crustacean species of Branchiopoda and Malacostraca. Bicistronic mRNA places Antp under the control of the Ubx promoter, which is active in the posterior segments of two branch...
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PMID: 16925676
PDF is available here.
PDF is available here.
Abstract
We report an extensive screen for ANTP class genes in Trichoplax, leading to isolation of three additional ANTP class genes. These can be assigned to the Dlx, Mnx and Hmx gene families. Sequencing approximately 12-20 kb around each gene indicates that none are part of tight gene clusters, and in sit...
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PMID: 16509895
PDF is available here.
PDF is available here.
Abstract
A growing number of oligopeptides of natural and/or synthetic origin have been described and considered as targeting structures for delivery bioactive compounds into various cell types. This review will outline the discovery of peptide sequences and the corresponding mid-sized oligopeptides with mem...
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PMID: 15952174
PDF is available here.
PDF is available here.
Mechanisms of Development (122)9 2005
Abstract
We have previously shown that the posterior part of the aorta is specified during embryogenesis to form the adult heart during metamorphosis, we determined if the embryonic aorta is also patterned by the function of Hox segmentation genes. Using gain- and loss-of-function experiments, we demonstrate...
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PMID: 15922572
PDF is available here.
PDF is available here.
Biochemistry (Washington) (44)31 2005
Abstract
We have previously identified a proregion-derived peptide (Pcp27), acting as a weak and reversible inhibitor of congopain. Pcp27 contains a 5-mer YHNGA motif, which is essential for selectivity in the inhibition of its mature form [Lalmanach, G., Lecaille, F., Chagas, J. R., Authié, E., Scharfstein...
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PMID: 16060657
PDF is available here.
PDF is available here.
Biochemistry (Washington) (44)30 2005
Abstract
We use high-resolution (1)H solution NMR methods to establish the location of the Antp(43-58) peptide bound to membrane bicelles composed of DMPC, DMPG, and DHPC, and compare it to the location of an Antp(43-58) variant which is not able to cross cell membranes. Two critical tryptophans are substitu...
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PMID: 16042388
PDF is available here.
PDF is available here.
Abstract
Arista versus tarsus determination is well investigated in Drosophila, yet it remains unresolved whether Antennapedia (ANTP) cell autonomously or noncell autonomously determines tarsus identity and whether Sex combs reduced (SCR) is the HOX protein required for normal tarsus determination. Three obs...
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PMID: 16094438
PDF is available here.
PDF is available here.
Abstract
Abstract
The results from these studies identified: (1) a cell trafficking domain and a cytotoxic domain for killing brain tumor cells; (2) that cell killing was independent of the domain orientations with regard to the cell trafficking domain being at the C-terminus or N-terminus; and (3) that the dual doma...
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PMID: 15832372
PDF is available here.
PDF is available here.
Abstract
We have proposed a 3D structure of Hoxc8-DNA complex obtained by Homology modeling and molecular dynamics (MD) simulation in explicit water. The crystal structure (9ant.pdb) of Antennapedia homeodomain in complex with its DNA sequence was chosen as the template based on (i) high sequence identity (8...
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PMID: 15842175
PDF is available here.
PDF is available here.
Cancer Cell (7)5 2005
Abstract
We engineered a cell-permeable peptidomimetic, shepherdin, modeled on the binding interface between the molecular chaperone Hsp90 and the antiapoptotic and mitotic regulator, survivin. Shepherdin makes extensive contacts with the ATP pocket of Hsp90, destabilizes its client proteins, and induces mas...
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PMID: 15894266
PDF is available here.
PDF is available here.
Abstract
We have reviewed the delivery of different molecules and particles mediated by TAT, Antp, VP22, and other CPPs as well as potential applications of these delivery systems in different areas of vaccine development, cancer immunotherapy, gene delivery, and cellular imaging....
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PMID: 15722168
PDF is available here.
PDF is available here.
Abstract
We carried out the structural determination of the recognition helix III in different solvent media. The conformational study of fragments 42-53, where residues W48 and F49, not involved in the protein-DNA interaction, were substituted by two alanines, was conducted in sodium dodecyl sulfate (SDS),...
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PMID: 15705164
PDF is available here.
PDF is available here.
Abstract
We report a new family of tetraguanidinium cell penetrating vectors efficiently internalized in human tumor cells. Their high internalization, studied by confocal microscopy and flow cytometry, as well as their specific accumulation in mitochondria makes these new vectors likely vehicles for the tar...
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PMID: 15656624
PDF is available here.
PDF is available here.
FASEB Journal (19)1 2005
Abstract
We recently established the critical role of the lipid phosphatase activity of the PTEN tumor suppressor in stabilizing cell-cell contacts and suppressing invasiveness. To delineate the effector systems involved, we investigated the interaction of PTEN with E-cadherin junctional complexes in kidney...
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PMID: 15629897
PDF is available here.
PDF is available here.
Development (132)1 2005
Abstract
In insects, selector genes are thought to modify the development of a default, or 'ground state', appendage into a tagma-specific appendage such as a mouthpart, antenna or leg. In the best described example, Drosophila melanogaster, the primary determination of leg identity is thought to result from...
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PMID: 15563520
PDF is available here.
PDF is available here.
Journal of Virology (78)24 2004
Abstract
We investigated the role of several PTD motifs in adenoviral infection. When physiologically expressed, a PTD from human immunodeficiency virus transactivator of transcription (Tat) did not improve adenoviral infection. We therefore fused PTDs to the ectodomain of the coxsackievirus-adenovirus recep...
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PMID: 15564483
PDF is available here.
PDF is available here.
Abstract
Four molecular dynamics simulation trajectories of complexes between the wild-type or a mutant Antennapedia homeodomain and 2 DNA sequences were generated in order to probe the mechanisms governing the specificity of DNA recognition. The starting point was published affinity measurements showing tha...
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PMID: 15468320
PDF is available here.
PDF is available here.
Biochemistry (Washington) (43)34 2004
Abstract
We revealed large dissimilarities in cell interactions among the peptides [Thorén et al. (2003) Biochem. Biophys. Res. Commun. 307, 100-107]. We here investigated possible correlations with their respective affinities for the lipid membranes of large unilamellar vesicles. The variations found in...
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PMID: 15323563
PDF is available here.
PDF is available here.
Developmental Biology (272)2 2004
Abstract
We show that the cells of the whole tube adopt the anterior aorta identity in the complete absence of Hox function. Conversely, ectopic expression of Antp, Ultrabithorax (Ubx), or abdominal-A (abd-A) transformed the anterior aorta into posterior cardiac tube by all available criteria, indicating an...
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PMID: 15282158
PDF is available here.
PDF is available here.
Abstract
Mechanisms of Development (121)6 2004
Abstract
We have studied the molecular and genetic basis of cross-regulatory interactions between labial and other more posterior Hox genes using the GAL4/UAS system for targeted misexpression. Misexpression of posterior Hox genes in the embryonic neuroectoderm results in a labial loss-of function phenotype...
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PMID: 15172684
PDF is available here.
PDF is available here.
Mechanisms of Development (121)5 2004
The Drosophila Polycomb group gene Sex combs extra encodes the ortholog of mammalian Ring1 proteins.
Abstract
We have characterized the Drosophila ortholog of the products of the mammalian Ring1/Ring1A and Rnf2/Ring1B genes. We show that Drosophila Ring corresponds to the Sex combs extra (Sce), a previously described PcG gene. We find that Ring/Sce is expressed and required throughout development and that t...
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PMID: 15147763
PDF is available here.
PDF is available here.
Journal of Virology (78)7 2004
Abstract
We introduced a series of mutations into the NS5B of an HCV subgenomic replicon and examined the replication capabilities of the resultant mutants by a colony formation assay. Replicons lacking the transmembrane domain did not yield any colonies. Furthermore, when Huh-7 cells harboring the HCV subge...
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PMID: 15016899
PDF is available here.
PDF is available here.
Abstract
The binding affinity for a 12-bp dsDNA of Antennapedia helix 3 analogues, major groove binders, has been measured by displacement of prebound ethidium bromide, a fluorescent displacement assay proposed for minor groove binders by Boger et al.(J. Am. Chem. Soc., 2000, 122, 6382-6394). Relative bindin...
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PMID: 15007422
PDF is available here.
PDF is available here.
Biochemistry (Washington) (43)11 2004
Abstract
We demonstrate the relationships between ligand binding and conformational stability using a previously designed protein, Ant-F, which undergoes a conformation change upon Zn(II) binding. To investigate the effect of stabilization of the apo structure on the conformation change, we also created a no...
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PMID: 15023058
PDF is available here.
PDF is available here.
Developmental Biology (267)2 2004
Abstract
We examine the role of Antennapedia in the establishment of leg identity in light of recent advances in our understanding of antennal development. In Antennapedia mutant clones in the leg disc, Homothorax and Distal-less are coexpressed and act via spineless to transform proximal femur to antenna. A...
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PMID: 15013806
PDF is available here.
PDF is available here.
Abstract
We demonstrated previously that inhibition of endothelial nitric oxide synthase (NOS), using pharmacological inhibitors, attenuated the ionomycin- and ATP-induced increases in microvessel permeability (Am J Physiol Heart Circ Physiol 272: H176-H185, 1997). Recently, the scaffolding domain of caveoli...
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PMID: 12946927
PDF is available here.
PDF is available here.
Nucleic Acids Research (32)22 2004
Abstract
We investigate the effect of cell-penetrating peptides on the biological activity of TFOs as measured in an assay for gene-targeted mutagenesis. Using the transport peptide derived from the third helix of the homeodomain of antennapedia (Antp), we tested TFO-peptide conjugates compared with unmodifi...
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PMID: 15602001
PDF is available here.
PDF is available here.
Biochemistry (Washington) (42)47 2003
Abstract
Our findings indicate that these relatively hydrophilic, polybasic cell-penetrating peptides can translocate through lipid bilayers by a potential- and composition-dependent pathway that causes only minimal perturbation to the overall integrity and barrier function of the bilayer....
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PMID: 14636045
PDF is available here.
PDF is available here.