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Fushi Tarazu Transcription Factors (0):
Recent article
Fushi Tarazu Transcription Factors
Abstract
We affinity purified Drosophila melanogaster spliceosomal B and C complexes formed in Kc cell nuclear extract. Mass spectrometry revealed that their composition is highly similar to that of human B and C complexes. Nonetheless, a number of Drosophila-specific proteins were identified, suggesting tha...
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PMID: 18981222
PDF is available here.
PDF is available here.
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Experimental Cell Research (314)4 2008
Abstract
Speckles are subnuclear domains where pre-mRNA splicing factors accumulate in the interchromatin space. To investigate the dynamics of mRNAs at speckles, fluorescently labeled Drosophila Fushitarazu (ftz) pre-mRNAs were microinjected into the nuclei of Cos7 cells and the dissociation kinetics of pre...
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PMID: 18053984
PDF is available here.
PDF is available here.
Abstract
We show that, despite apical transport of mRNA being highly directional, mRNA particles often pause and move backward toward the plus ends of microtubules. We suggest that this retrograde movement helps overcome cellular obstructions. We show that the plus end movement of apical mRNA is independent...
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PMID: 17901156
PDF is available here.
PDF is available here.
Abstract
These results indicated that responsiveness of sFF1-I and ERalpha genes to sGnRH and sex steroid hormones is seasonally variable in relation to reproductive stages. Expression of sFF1 and ERalpha genes should be stimulated at the early stages of gonadal maturation prior to increases in the amounts o...
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PMID: 17418845
PDF is available here.
PDF is available here.
Abstract
We have studied the expression of orthologs of zen (DpuHox3) and ftz (Dpuftz) in the crustacean Daphnia pulex (Branchiopoda), both of which show a dynamic expression pattern. DpuHox3 is expressed in a complex pattern in early embryogenesis, with the most anterior boundary of expression lying at the...
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PMID: 17310351
PDF is available here.
PDF is available here.
Developmental Biology (301)2 2007
Abstract
We identified lilli in a genetic screen based on the suppression of a lethal phenotype that is associated with ectopic expression of the transcription factor encoded by the segmentation gene runt in the blastoderm embryo. In contrast to other factors identified by this screen, lilli appears to have...
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PMID: 17137570
PDF is available here.
PDF is available here.
Abstract
We identified a new member of ftz-f1 gene in Schistosoma mansoni, termed Smftz-f1alpha. The Smftz-f1alpha gene has a complex structure with 15 exons interrupted by 14 introns. It encodes an unusually long SmFTZ-F1alpha protein of 1892 amino acids containing all the modular domains found in nuclear r...
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PMID: 16870276
PDF is available here.
PDF is available here.
Abstract
We characterize the DNA binding properties and the transactivation activity of SmFTZ-F1alpha. In in vitro assays, SmFTZ-F1alpha bound as a monomer to a response element (FF1RE: TCAAGGTCA) recognized by mammalian steroidogenic factor 1 (SF-1), and to related sequences (p14: TTAAGGTCA and SmFF1a-2: CG...
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PMID: 16890303
PDF is available here.
PDF is available here.
Developmental Biology (299)1 2006
Abstract
We have made use of the fact that the DNA binding specificity of the Drosophila Hox protein Fushi Tarazu (Ftz) is modulated by interaction with its partner, the orphan nuclear receptor Ftz-F1, to carry out a computational screen for genomic targets. At least two of the first 30 potential target gene...
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PMID: 16996052
PDF is available here.
PDF is available here.
Abstract
We report that ZFP TFs can regulate gene expression in transgenic Drosophila. To demonstrate this, we targeted the promoter of the well-characterized fushi tarazu (ftz) gene with a ZFP TF activator using the VP16 activation domain from Herpes simplex virus, and ZFP TF repressors using the Drosophila...
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PMID: 16899226
PDF is available here.
PDF is available here.
Journal of Cell Science (119)Pt 18 2006
Abstract
We present the first analysis of the dynamics of the transcription DNA-repair factor TFIIH at the onset of transcription in early Drosophila development. TFIIH is composed of ten polypeptides that are part of two complexes - the core and the CAK. We found that the TFIIH core is initially located in...
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PMID: 16940351
PDF is available here.
PDF is available here.
Development (133)18 2006
Abstract
We have used a 'ligand sensor' system to visualize spatial activity patterns for each of the 18 Drosophila nuclear receptors in live developing animals. Transgenic lines were established that express the ligand binding domain of each nuclear receptor fused to the DNA-binding domain of yeast GAL4. Wh...
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PMID: 16914501
PDF is available here.
PDF is available here.
Abstract
We have screened a yeast two-hybrid adult worm cDNA library using a construct expressing the D, E and F domains of SmFtz-F1 as bait. One of the selected clones encoded a sequence without homologues in any other species, apart from Schistosoma japonicum. The complete sequence was obtained by 5' and 3...
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PMID: 16574255
PDF is available here.
PDF is available here.
Genes to Cells (11)3 2006
Abstract
We microinjected fluorescently labeled fushi tarazu (ftz) pre-mRNA into the nuclei of HeLa cells. The injected intron-containing ftz pre-mRNA was distributed to the SC35 speckles and exported to the cytoplasm after splicing by an energy-requiring active process. In contrast, the injected intron-less...
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PMID: 16483318
PDF is available here.
PDF is available here.
Journal of Molecular Biology (354)5 2005
Abstract
We present the 2.2A resolution crystal structure of the hLRH-1 DBD in complex with duplex DNA, and elucidate the sequence-specific DNA contacts essential for the ability of LRH-1 to bind to DNA as a monomer. We show that the unique Ftz-F1 domain folds into a novel helix that packs against the DBD bu...
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PMID: 16289203
PDF is available here.
PDF is available here.
Journal of Neuroscience (25)40 2005
Abstract
We have used whole-cell patch-clamp recording, pharmacology, immunohistochemistry, and genetic markers to investigate how GABAergic inhibition affects olfactory processing in the Drosophila antennal lobe. We show that many axonless local neurons (LNs) in the adult antennal lobe are GABAergic. GABA h...
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PMID: 16207866
PDF is available here.
PDF is available here.
Current Biology (15)7 2005
Abstract
We found that Dm-ftz mediates segmentation but not homeosis, whereas orthologs from grasshopper (Sg-ftz) and beetle (Tc-Ftz), both containing a YPWM motif, have homeotic function. Tc-Ftz, which unlike Sg-Ftz contains an LXXLL motif, displays stronger segmentation function than Sg-Ftz. Cofactor-inter...
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PMID: 15823536
PDF is available here.
PDF is available here.
Journal of Neurogenetics (19)3-4 2005
Abstract
We evaluated the role of IP(3) in sugar taste reception in Drosophila melanogaster by inactivating the IP(3) signaling using genetic tools. We used the "IP(3) sponge," composed of the modified ligand-binding domain from the mouse IP(3) receptor, which was designed to absorb IP(3) in competition with...
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PMID: 16540404
PDF is available here.
PDF is available here.
Abstract
Sex determination is the process deciding the sex of a developing embryo. This is usually determined genetically; however it is a delicate process, which in many cases can be influenced by environmental factors. The mechanisms controlling zebrafish sex determination and differentiation are not known...
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PMID: 16281973
PDF is available here.
PDF is available here.
Abstract
We show that DPM neuron output is only required during the consolidation phase for middle-term odor memory and is dispensable during acquisition and recall. However, we found that DPM neuron output is required during acquisition of a benzaldehyde odor memory. We show that flies sense benzaldehyde by...
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PMID: 15504331
PDF is available here.
PDF is available here.
Abstract
We examined the molecular basis that generates distinct morphological classes of Drosophila dendritic arborization (da) neurons. da neurons are classified into classes I to IV in order of increasing territory size and/or branching complexity. We found that Abrupt (Ab), a BTB-zinc finger protein, is...
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PMID: 15363392
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (279)37 2004
Abstract
We investigated modifications that control SF-1 function and found that SF-1 could be conjugated by SUMO-1 both in vitro and in vivo. SF-1 was modified predominantly at Lys(194) and much less at Lys(119) when free SUMO-1 was supplied. Mutations of Lys(194) and Lys(119) enhanced transcriptional activ...
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PMID: 15192092
PDF is available here.
PDF is available here.
Abstract
We conducted a screen to identify dominant mutations that enhance the lethality of a ftz temperature-sensitive mutant. Twenty-six enhancers were isolated, which define 21 genes. All but one of the mutations recovered show a maternal effect in their interaction with ftz. Three of the enhancers proved...
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PMID: 15454535
PDF is available here.
PDF is available here.
Abstract
We characterized the roles of NHR-25, a member of the nuclear receptor family of transcription factors. The NHR-25 homologs Ftz-F1 in Drosophila and SF-1 in mammals are involved in various biological processes, including regulation of patterning during development, reproduction, metabolism, metamorp...
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PMID: 15314147
PDF is available here.
PDF is available here.
Biochemical Journal (382)Pt 1 2004
Abstract
We have characterized the roles of these specific regions both in the transcriptional activity of the receptor and in its interactions with cofactors. Our results have shown that, although the AF-2 (activation function-2) region is the major activation function of the receptor, both the F and D doma...
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PMID: 15104535
PDF is available here.
PDF is available here.
Abstract
A FTZ-F1 homologue was cloned from the pituitary cDNA library of the orange-spotted grouper. The full-length cDNA of the orange-spotted grouper FTZ-F1 spanned 1735 bp including a poly (A) tail. The open reading frame encodes a protein of 468 amino acids. Sequence analysis indicated t...
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PMID: 15286949
PDF is available here.
PDF is available here.
Abstract
We found that insulin stimulated (2-4 fold) the activity of this promoter in a time- and concentration-dependent manner. Accordingly, insulin up-regulated the level of LHbeta mRNA. In contrast, insulin had no significant effect on GnRH-dependent LHbeta expression. The expression of Egr-1 and SF-1, w...
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PMID: 15295353
PDF is available here.
PDF is available here.
Chinese Medical Journal (117)7 2004
Abstract
Activation of PKA causes wild type, but not mutant SF-1 to alter its subnuclear distribution pattern to a transactivationally active form (foci formation). This finding may throw new light on the mechanism by which PKA activates the orphan nuclear receptor....
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PMID: 15265375
PDF is available here.
PDF is available here.
Endocrinology (145)7 2004
Abstract
We report here the results of the analysis of aromatase and steroidogenic factor 1 (Sf1) expression in adult lung of the urodele amphibian Pleurodeles waltl. Using RT-PCR experiments, we show the expression of the estrogen-synthesizing enzyme, aromatase, in this organ. In the lung, no significant di...
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PMID: 15070855
PDF is available here.
PDF is available here.
Abstract
The significance of steroidogenic factor 1 (SF-1) in adrenal steroidogenesis was studied using adrenocortical cell lines transformed with a dominant negative mutant of SF-1. Constitutive expression of the mutant did not only impair the activity of endogenous SF-1 but also diminish its own expression...
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PMID: 15261303
PDF is available here.
PDF is available here.
Journal of Endocrinology (181)2 2004
Abstract
We examined the capacity of human adrenocortical cells to produce cortisol and androgens in response to these secretagogues and their ability to regulate the expression of CYP21 and CYP17. In H-295 cells, forskolin and A-II were found to stimulate production of cortisol relative to androstenedione a...
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PMID: 15128284
PDF is available here.
PDF is available here.
Abstract
We will describe these aspects regarding the pathology of adrenal incidentaloma in this review with emphasis on the three aspects above....
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PMID: 15148819
PDF is available here.
PDF is available here.
Endocrinology (145)5 2004
Abstract
We hypothesized that liver receptor homolog-1 (LRH-1) (nuclear receptor 5A2), a receptor closely related to SF-1, could also play a role in regulating aromatase expression in the testis. We demonstrate expression of LRH-1 in adult rat and immature mouse Leydig cells (LHR-1 > SF-1) as well as in pach...
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PMID: 14736734
PDF is available here.
PDF is available here.
Development (131)10 2004
Abstract
We have used an ectopic expression system that allows for quantitative manipulation of expression levels to probe the role of the primary pair-rule transcription factor Runt in segment-polarity gene regulation. These experiments identify sloppy paired 1 (slp1) as a gene that is activated and repress...
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PMID: 15102703
PDF is available here.
PDF is available here.
Abstract
We now describe a novel 8-bp microdeletion of SF-1, isolated from a 46, XY patient who presented with gonadal agenesis but normal adrenal function, which causes premature termination upstream of sequences encoding the activation function 2 domain. In cell transfection experiments, the mutated protei...
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PMID: 15070943
PDF is available here.
PDF is available here.
Molecular Endocrinology (18)4 2004
Abstract
We examined mechanisms underlying SF-1 haploinsufficiency and found a striking reduction (12-fold) in SF-1 heterozygous (+/-) adrenocortical size at embryonic day (E) 12. Loss of one SF-1 allele led to a selective decrease in adrenal precursors within the adrenogonadal primordium at E10.0, without a...
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PMID: 14726490
PDF is available here.
PDF is available here.
Abstract
Endocrinology (145)3 2004
Abstract
We determined that DBP treatment led to diminished transport of cholesterol across the mitochondrial membrane as well as diminished function at each point in the testosterone biosynthesis pathway except 17 beta-hydroxysteroid dehydrogenase. The transcriptional repression caused by DBP does not appea...
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PMID: 14617579
PDF is available here.
PDF is available here.
Molecular and Cellular Endocrinology (215)1-2 2004
Abstract
We have sought to make tissue-specific SF-1 KO mice (as reviewed here). We first used adrenal transplants to restore adrenal function in global SF-1 KO mice, providing a physiological form of a "VMH-specific" KO to study the roles of SF-1 in weight regulation. These adrenal-transplanted SF-1 KO mice...
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PMID: 15026179
PDF is available here.
PDF is available here.
Abstract
We demonstrate that the transcriptional co-activator p300 regulates this synergy. Overexpression of p300 results in strong stimulation of LHbeta promoter but only in the presence of both SF-1 and Egr-1, and not in the presence of other Egr proteins. Mutation of the binding sites for either SF-1 or E...
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PMID: 14681221
PDF is available here.
PDF is available here.
Abstract
Abstract
Abstract
We demonstrated that there is interaction between SF-1 and Sp1 on the human StAR promoter. In the present study, we examined the mechanism of the interaction between Sp1 and SF-1 on the human StAR gene promoter. Results of glutathione S-transferase (GST) pull-down assays and a mammalian two-hybrid a...
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PMID: 15129808
PDF is available here.
PDF is available here.
Abstract
Sex determination and differentiation depend on differentiation of the indifferent gonad to the testis or ovary, which leads to masculine or feminine differentiation of internal and external genitalia. Recently, genes involved in this cascade have been identified with the advance of molecular genetic...
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PMID: 14968537
PDF is available here.
PDF is available here.
Abstract
Internal sexual organs are differentiated and developed by androgens and regressed by müllerian inhibiting substance(anti-müllerian hormone). The role of 5 alpha-dihydrotestosterone, reduced form of testosterone by 5 alpha-reductase, in terms of development of Wolffian duct is discussed with solubl...
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PMID: 14968531
PDF is available here.
PDF is available here.
Endocrinology (145)2 2004
Abstract
We provide evidence that its activity in adrenocortical cells depends on the binding of the adrenal-specific factor, which is required for basal and cAMP-induced promoter activity. Furthermore, the -503 site has intrinsic cAMP-sensing ability in Y1 cells, which is correlated with increased adrenal-s...
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PMID: 14605009
PDF is available here.
PDF is available here.
Abstract
We report on the artificial transfer of two Wolbachia variants (wCer1 and wCer2) from R. cerasi into Drosophila simulans, which was performed with two major goals in mind: first, to isolate wCer1 from wCer2 in order to individually test their respective abilities to induce CI in the new host; and, s...
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PMID: 14711652
PDF is available here.
PDF is available here.
Abstract
We have used two complementary in vivo and in vitro approaches. Firstly, we have isolated the pituitary promoter of the rat GnRH receptor gene and investigated its activity using transient transfection into two gonadotrope-derived cell lines, the alphaT3-1 and the LbetaT2 cell lines. We have thus de...
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PMID: 15146959
PDF is available here.
PDF is available here.
Biological Chemistry (385)1 2004
Abstract
The functional insect ecdysteroid receptor is comprised of the ecdysone receptor (EcR) and Ultraspiracle (USP). The ligand-binding domain (LBD) of USP was fused to the GAL4 DNA-binding domain (GAL4-DBD) and characterized by analyzing the effect of site-directed mutations in the LBD. Normal and mutan...
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PMID: 14977043
PDF is available here.
PDF is available here.
Genome Biology (5)9 2004
Abstract
Measuring conservation of sequence features closely linked to function--such as binding-site clusterin--makes better use of comparative sequence data than commonly used methods that examine only sequence identity....
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PMID: 15345045
PDF is available here.
PDF is available here.
Abstract
We analyzed their expression in a primitive insect, the firebrat Thermobia domestica. The expression patterns seem to represent a stage of evolution intermediate between the ancestral state seen in basal arthropods and the derived expression patterns in Drosophila. These expression data help us to n...
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PMID: 15509221
PDF is available here.
PDF is available here.
Abstract
The expression of cytochrome P450scc, encoded by the CYP11A gene, was investigated in the rat kidney from birth to adulthood. In the male and female rat kidneys, the corresponding mRNA was detected by semi-quantitative reverse transcriptase and polymerase chain reaction (RT-PCR) analysis with specif...
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PMID: 15026086
PDF is available here.
PDF is available here.
Molecular Endocrinology (18)1 2004
Abstract
We investigated the mechanism by applying laser confocal microscopy and fluorescence recovery after photobleaching technique. In KGN cells, forskolin (a PKA stimulator) could reorganize wild-type Ad4BP/SF-1, but not mutant Ad4BP/SF-1 (G35E), from a diffuse distribution pattern to foci formation in t...
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PMID: 14555713
PDF is available here.
PDF is available here.
Molecular and Cellular Endocrinology (211)1-2 2003
Abstract
We have shown that MIS inhibits the cAMP-induced expression of cytochrome P450 C17alpha-hydroxylase/C17-20 lyase (Cyp17) mRNA both in vitro and in vivo. Our current efforts are to understand the molecular mechanisms regulating both MIS type II receptor (MISRII) expression and its signaling in rodent...
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PMID: 14656482
PDF is available here.
PDF is available here.
Abstract
Abstract
We report the first functional characterization of a new variation found in the hinge region of human Ad4BP/SF-1, G146A. Resulting from a single nucleotide shift (GGG-->GCG), G146A bears slightly diminished transactivation activity evidenced by both adrenal specific cyp11A promoter and ovary specifi...
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PMID: 14623279
PDF is available here.
PDF is available here.
Biology of Reproduction (69)6 2003
Abstract
We show by gel shift and chromatin immunoprecipitation assays that WT1 bound to a GC-rich sequence in the murine Mis promoter. Mutation in this site abolished WT1-mediated activation of the Mis promoter. The WT1, SRY box protein 9, and steroidogenic factor 1 could synergistically activate the Mis pr...
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PMID: 12855602
PDF is available here.
PDF is available here.
Mechanisms of Development (120)12 2003
Abstract
We analyze the phenotypes of ectopic expression of FTZ and FTZ protein deletions from the Tubulin alpha1 (Tubalpha1) promoter. Fusion of ftz to the Tubalpha1 promoter resulted in low-level ectopic expression of FTZ relative to FTZ expressed from the endogenous ftz gene. The effects of ectopic expres...
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PMID: 14654217
PDF is available here.
PDF is available here.
Abstract
In anautogenous mosquitoes, vitellogenesis, which includes production of yolk protein precursors, requires blood feeding. Consequently, mosquitoes transmit many diseases. Understanding the molecular mechanisms of vitellogenesis regulation will contribute significantly to vector control strategies. N...
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PMID: 14593204
PDF is available here.
PDF is available here.
Journal of Endocrinology (179)2 2003
Abstract
We demonstrate that SF-1 significantly enhances basal and forskolin-stimulated transcription of the human alphaGSU promoter in GH(3) cells. Mutation of the GSE abolished the SF-1-mediated transactivation of basal alphaGSU promoter activity, and significantly attenuated the forskolin effect by 50%. M...
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PMID: 14596681
PDF is available here.
PDF is available here.