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Nuclear Factor 90 Proteins (1):
Recent article
Nuclear Factor 90 Proteins
Biochemistry (Washington) (47)32 2008
Abstract
We show that the Xenopus orthologue of ILF3 (Xilf3) is methylated in vivo, and, at least in vitro, this methylation is carried out by Xprmt1b. The in vitro methylation of Xilf3 inhibits its ability to bind to DNA while leaving RNA binding activity unaltered. Consistent with these activities having a...
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PMID: 18636753
PDF is available here.
PDF is available here.
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Abstract
We show that heterodimeric core complexes, NF90-NF45 and NF110-NF45, exist within larger complexes that are more labile and contain multiple NF90/110 isoforms and additional proteins. Depletion of the NF45 subunit by RNA interference is accompanied by a dramatic decrease in the levels of NF90 and NF...
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PMID: 18458058
PDF is available here.
PDF is available here.
Abstract
We examine the posttranscriptional events underlying the robust MKP-1 induction by oxidants in HeLa cells. H(2)O(2) treatment potently stabilized the MKP-1 mRNA and increased the association of MKP-1 mRNA with the translation machinery. Four RNA-binding proteins (RNA-BPs) that influence mRNA turnove...
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PMID: 18490444
PDF is available here.
PDF is available here.
Biological Chemistry (389)3 2008
Abstract
We review recent progress in our knowledge of the turnover and translation regulatory (TTR) mRNA-binding proteins (RBPs) that influence gene expression in response to oxidative damage. Specifically, we identify oxidant damage-regulated mRNAs that are targets of TTR-RBPs, we review the oxidant-trigge...
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PMID: 18177264
PDF is available here.
PDF is available here.
Physiological Genomics (32)2 2008
Abstract
These results provide evidence of epigenetic differences between male and female bovine in vitro produced embryos and suggest that before initiation of gonadal differentiation, epigenetic events may modulate the difference between speed of development, metabolism, and transcription observed during p...
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PMID: 17986520
PDF is available here.
PDF is available here.
Journal of Immunology (180)1 2008
Abstract
We demonstrate that AKT phosphorylates NF90 on Ser647 upon CD28 costimulation. This phosphorylation is necessary for nuclear export of NF90 and IL-2 mRNA stabilization by this protein, because a mutation at Ser647 abolished both functions. We observed that treatment of cells with CD28 costimulation...
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PMID: 18097023
PDF is available here.
PDF is available here.
Journal of Andrology (29)2 2008
Abstract
We utilized the mouse SP-10 gene encoding a conserved acrosomal protein as an experimental model. Promoter analysis in transgenic mice had previously shown that the -186/-91 region of the SP-10 promoter was critical for spermatid-specific expression. Here, we focus on a purine (Pu) box (-agaaaa) loc...
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PMID: 17942973
PDF is available here.
PDF is available here.
Abstract
Vascular endothelial growth factor (VEGF) is a key angiogenic factor expressed under restricted nutrient and oxygen conditions in most solid tumors. The expression of VEGF under hypoxic conditions requires transcription through activated hypoxia-inducible factor 1 (HIF-1), increased mRNA stability,...
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PMID: 18039850
PDF is available here.
PDF is available here.
Experimental Cell Research (313)20 2007
Abstract
We identified 11 proteins that are associated with DGCR8 by immunoprecipitation assay and mass spectrometry. These proteins included Nucleolin, ILF3 and others, most of which appeared to be involved in the RNA processing or RNA transportation. We detected at least four kinds of protein complex, such...
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PMID: 17765891
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1774)10 2007
Abstract
We show that the human protein, ZAP3 (also known as ZAP), is localized to the nucleus, that it is expressed in all mammalian tissues examined, and docks to PP1 through an RVRW motif located in its highly conserved carboxy-terminus. Proteomic analysis of a ZAP3 complex revealed that in addition to bi...
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PMID: 17890166
PDF is available here.
PDF is available here.
Abstract
Activation of T cells induces the production of T cell growth and survival factor interleukin (IL) 2. Regulatory T cells intrinsically fail to induce IL-2 expression upon activation and can suppress IL-2 production in conventional T cells. Thus, the control of IL-2 expression is critically important...
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PMID: 17470640
PDF is available here.
PDF is available here.
Methods in Enzymology (429) 2007
Abstract
We employ chemical crosslinking of the RNA and protein moieties. Here we describe the identification of cellular RNAs bound to nuclear factor 90 (NF90), the founder member of a family of ubiquitous double-stranded RNA-binding proteins. Crosslinked RNA-NF90 complexes were immunoprecipitated from stab...
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PMID: 17913627
PDF is available here.
PDF is available here.
Nucleic Acids Research (35)7 2007
Abstract
IL-2 gene expression in activated T-cells is initiated by chromatin remodeling at the IL-2 proximal promoter and conversion of a transcriptional repressor into a potent transcriptional activator. A purine-box regulator complex was purified from activated Jurkat T-cell nuclei based on sequence-specif...
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PMID: 17389650
PDF is available here.
PDF is available here.
Nucleic Acids Research (35)18 2007
Abstract
We discovered a family of small NF90-associated RNAs (snaR). These highly structured non-coding RNAs of approximately 117 nucleotides are expressed in immortalized human cell lines of diverse lineages. In human tissues, they are abundant in testis, with minor distribution in brain, placenta and some...
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PMID: 17855395
PDF is available here.
PDF is available here.
Abstract
We determined the exon-intron organization of the entire mouse Ilf3 gene divided into 22 exons....
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PMID: 16952437
PDF is available here.
PDF is available here.
Abstract
Double-stranded RNA-activated protein kinase (PKR) plays an important role in interferon-induced antiviral responses, and is also involved in intracellular signaling pathways, including the apoptosis, proliferation, and transcription pathways. In this study, a new 120-kDa PKR-associated protein desi...
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PMID: 16861808
PDF is available here.
PDF is available here.
Biochemical Journal (396)1 2006
Abstract
The 3'-UTR (untranslated region) of bcl-2 mRNA contains an ARE (AU-rich element) that potentially regulates the stability of bcl-2 mRNA in a cell specific fashion. Previous studies have demonstrated that multiple proteins interact with bcl-2 mRNA in HL-60 (human leukaemia-60) cells, potentially cont...
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PMID: 16396631
PDF is available here.
PDF is available here.
Retrovirology (3)1 2006
Abstract
Since Rev:RRE complex formations depend on protein:RNA and protein:protein interactions, we investigated whether the expression of NF90ctv might interfere with Rev-mediated export of RRE-containing transcripts. When HeLa cells expressed both NF90ctv and Rev protein, we observed that NF90ctv inhibite...
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PMID: 17125513
PDF is available here.
PDF is available here.
Abstract
We report a novel function of ADAR1 in the regulation of gene expression by interacting with the nuclear factor 90 (NF90) proteins, known regulators that bind the antigen response recognition element (ARRE-2) and have been demonstrated to stimulate transcription and translation. ADAR1 upregulates NF...
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PMID: 16055709
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (280)19 2005
Abstract
NF90 and splice variant NF110/ILF3/NFAR are double-stranded RNA-binding proteins that regulate gene expression. Mice with targeted disruption of NF90 were engineered. NF90(-/-) mice were born small and weak and succumbed to perinatal death within 12 h because of neuromuscular respiratory failure. Lu...
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PMID: 15746098
PDF is available here.
PDF is available here.
Experimental Cell Research (305)2 2005
Abstract
NF45/ILF2 associates with NF90/ILF3 in the nucleus and regulates IL-2 gene transcription at the antigen receptor response element (ARRE)/NF-AT DNA target sequence (P.N. Kao, L. Chen, G. Brock, J. Ng, A.J. Smith, B. Corthesy, J. Biol. Chem. 269 (1994) 20691-20699). NF45 is widely expressed in normal...
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PMID: 15817156
PDF is available here.
PDF is available here.
Journal of Molecular Biology (348)2 2005
Abstract
We investigated the mechanism of translocation for NF90 and NF110, its larger splice variant. During interphase, NF90 is predominantly nuclear, NF110 is exclusively nuclear, and both are bound to RNA. About half of the NF90 is tethered in the nucleus by RNA bound to the protein's dsRNA-binding motif...
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PMID: 15811368
PDF is available here.
PDF is available here.
FASEB Journal (18)14 2004
Abstract
We have detected several proteins that interact with the targeting RNA element and could potentially be involved in Tau mRNA translocation, translation halting, and/or stabilization. Among them, two proteins were identified as the interleukin enhancer binding factor 3 (Ilf3) and NF90, two isoforms d...
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PMID: 15364895
PDF is available here.
PDF is available here.
Abstract
We found that JAZ binds to exportin-5 in a Ran-GTP- and dsRNA-dependent manner. Exportin-5 stimulates JAZ shuttling, and gene silencing of exportin-5 reduces shuttling. Recombinant exportin-5 also stimulates nuclear export of JAZ in permeabilized cells. JAZ also binds to ILF3, and surprisingly, this...
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PMID: 15254228
PDF is available here.
PDF is available here.
Molecular Cell (13)1 2004
Abstract
We have identified RHAU, a putative DExH RNA helicase that was isolated in association with the ARE of urokinase plasminogen activator mRNA (ARE(uPA)). RHAU physically interacts with the deadenylase PARN and the human exosome and enhances the deadenylation and decay of ARE(uPA)-mRNAs. An alternative...
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PMID: 14731398
PDF is available here.
PDF is available here.
Abstract
We show that the double-stranded RNA-binding protein, ILF3 (interleukin enhancer binding factor 3) preferentially binds minihelix motif. Gel retardation assays and glutathione S-transferase pull-down experiments revealed that ILF3, exportin-5, RanGTP, and VA1 RNA assembled in a quaternary complex in...
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PMID: 14570900
PDF is available here.
PDF is available here.
Abstract
TCP80 is an approximately 80kDa mammalian cytoplasmic protein that binds to a set of mRNAs and inhibits their translation in vitro and ex vivo. This protein has high sequence similarity to interleukin-2 enhancer-binding factors (NF90/ILF3) and the M-phase phosphoprotein (MPP4)/DRBP76. A 110kDa immun...
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PMID: 14654356
PDF is available here.
PDF is available here.
EMBO Journal (22)21 2003
Abstract
We describe a group of host factors designated as 'NFAR proteins' that are recruited by the replication machinery of bovine viral diarrhea virus, a close relative of the human pathogen hepatitis C virus. The NFAR proteins associate specifically with both the termini of the viral RNA genome involving...
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PMID: 14592965
PDF is available here.
PDF is available here.
Journal of Molecular Biology (332)1 2003
Abstract
We verified the expression of four of the isoforms by cDNA cloning and mass spectrometric analysis of proteins isolated from human cells. Cell fractionation studies showed that NF90 and its heteromeric partner, NF45, are predominantly nuclear and largely chromatin-associated. The C-terminally extend...
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PMID: 12946349
PDF is available here.
PDF is available here.
Abstract
We investigated whether accumulation of dsRNA in VV-infected cells could regulate IL-2 gene expression. Infection of Jurkat cells with a VV mutant that produces free dsRNA led to similar levels of induced NF-90 within the cell, but the protein remained localized within the cytosol. This mutant did n...
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PMID: 14565858
PDF is available here.
PDF is available here.
Abstract
We can say U5 small nuclear ribonucleoprotein-specific 40 kDa protein appears to play important role in spliceosome assembly and disassembly, whereas La protein is involved in small nuclear riboncleoprotein complex biogenesis and transfer RNA maturation. Aberrant expression of these proteins points...
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PMID: 14528909
PDF is available here.
PDF is available here.
Abstract
We find that dsRNA binding is reduced by RGG and GQSY motifs present in the C-terminal region. To directly evaluate the role of RNA binding in transactivation, we conducted site-directed mutagenesis to substitute conserved residues in one or both of the dsRBMs. The mutations reduced the ability of N...
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PMID: 12702813
PDF is available here.
PDF is available here.
Genes & Development (17)8 2003
Abstract
We report that the sequence-specific DNA-binding transcription factor Yin Yang 1 (YY1) binds to and recruits the histone H4 (Arg 3)-specific methyltransferase, PRMT1, to a YY1-activated promoter. Our data confirm that histone methylation does not occur randomly but rather is a targeted event and pro...
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PMID: 12704081
PDF is available here.
PDF is available here.
Nucleic Acids Research (31)5 2003
Abstract
We previously showed that these proteins co-localise in the nucleus of interphase cells. In this study we show that p68 and p72 can interact with each other and self-associate in the yeast two-hybrid system. Co-immunoprecipitation experiments confirmed that p68 and p72 can interact in the cell and i...
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PMID: 12595555
PDF is available here.
PDF is available here.
Molecular Cell (10)6 2002
Abstract
In response to T cell activation signals, the half-life of interleukin-2 (IL-2) mRNA is greatly extended. The cis elements mediating IL-2 mRNA stabilization are located in its 5' and 3' untranslated regions (UTR). The 3'UTR also contains AU-rich elements (AREs) that mediate rapid IL-2 mRNA degradati...
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PMID: 12504009
PDF is available here.
PDF is available here.
Abstract
We show that a C-terminal variant of the ubiquitous dsRNA-binding protein, nuclear factor 90 (NF90ctv), can activate the interferon response genes in the absence of viral infection. NF90ctv-expressing cells were infected with the syncytium-inducing HIV-1 strain NL4-3 and were shown to inhibit viral...
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PMID: 12036489
PDF is available here.
PDF is available here.
Archives of Virology (147)3 2002
Abstract
We report that two HBV epsilon RNA-binding host proteins (80 and 43 kDa) and a copurifying protein (100 kDa) were purified and characterized by the combined methods of UV cross-linking analysis with the epsilon RNA and column chromatography. Amino-terminal microsequencing showed that 80- and 43-kDa...
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PMID: 11958450
PDF is available here.
PDF is available here.
Journal of Cell Biology (156)1 2002
Abstract
We have identified a novel human karyopherin (Kap) beta family member that is related to human Crm1 and the Saccharomyces cerevisiae protein, Msn5p/Kap142p. Like other known transport receptors, this Kap binds specifically to RanGTP, interacts with nucleoporins, and shuttles between the nuclear and...
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PMID: 11777942
PDF is available here.
PDF is available here.
Abstract
We examined its ability to modulate promoter function in cellular transfection assays. NF90-Gal4 fusion proteins inhibited transcription from the adenovirus major late promoter in a fashion that was dependent on Gal4 targeting. Conversely, NF90 activated the cytomegalovirus immediate-early promoter,...
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PMID: 11739746
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (276)35 2001
Abstract
We characterize the biochemical interactions between these two dsRBM containing proteins. NF90 binds to PKR through two independent mechanisms: an RNA-independent interaction occurs between the N terminus of NF90 and the C-terminal region of PKR, and an RNA-dependent interaction is mediated by the d...
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PMID: 11438540
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (276)34 2001
Abstract
We report here the isolation and characterization of two proteins, NFAR-1 and -2, which were isolated through their ability to interact with the dsRNA-dependent protein kinase, PKR. The NFAR proteins, of 90 and 110 kDa, are derived from a single gene through alternative splicing and are evolutionari...
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PMID: 11438536
PDF is available here.
PDF is available here.
Abstract
We have shown is a substrate for the interferon-inducible, dsRNA-dependent protein kinase, PKR. However, our protein expression analysis has revealed that NFAR exists as two major protein species of 90 kDa (NFAR-1) and 110 kDa (NFAR-2) in the cell. To resolve the genetic identity of NFAR-1 and -2, w...
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PMID: 11161820
PDF is available here.
PDF is available here.
Abstract
We isolated several ilf3 transcripts from a melanoma cDNA library and two corresponding genomic fragments, and report alternative splicing and polyadenylation site selection in the human ILF3 gene. We show the existence of an alternative splice site responsible for the sequence divergence in the 3'...
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PMID: 11167023
PDF is available here.
PDF is available here.
Life Sciences (67)8 2000
Abstract
We have examined differential gene expression in non-malignant and malignant nasopharyngeal epithelial (NPE) cells using a cDNA array hybridization method. A total of 42 genes were identified to be expressed in either non-malignant and malignant NPE cells or both. Thirteen of these genes were overex...
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PMID: 10946852
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (275)26 2000
Abstract
We used the yeast two-hybrid system to identify proteins that interact with PRMT1. One of the interacting proteins we cloned is interleukin enhancer-binding factor 3 (ILF3), also known as M phase phosphoprotein 4. ILF3 is closely related to nuclear factor 90 (NF90). Using an immunofluorescence analy...
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PMID: 10749851
PDF is available here.
PDF is available here.
Abstract
These results show that the multifunctional protein, TCP80/NF90, can function in vivo as a translation inhibitory protein and include alterations of mRNA binding to polysomes as a component of its mechanism of action.
Copyright 2000 Academic Press....
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PMID: 10873392
PDF is available here.
PDF is available here.
Abstract
Acid beta-glucosidase (GCase) is the enzyme deficient in Gaucher disease, a prototypical inherited metabolic error for enzyme and gene therapy. An 80-kDa cytoplasmic protein, termed TCP80, was found to inhibit GCase mRNA translation in mammalian cells by binding to RNA-coding regions. The TCP80 cDNA...
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PMID: 10607473
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (274)29 1999
Abstract
We report the purification of DRBP76, a new human member of this class of proteins. Sequence from the amino terminus of DRBP76 matched that of the M phase-specific protein, MPP4. DRBP76 was also cloned by the yeast two-hybrid screening of a cDNA library using a mutant PKR as bait. Analysis of the cD...
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PMID: 10400669
PDF is available here.
PDF is available here.
Biochemistry (Washington) (38)19 1999
Abstract
We have demonstrated that NF90 specifically binds to double-stranded RNA. Furthermore, NF90 was phosphorylated in a double-stranded RNA-dependent manner likely by the interferon-induced, double-stranded RNA-dependent protein kinase, PKR. The NF90 protein was found to be expressed not only in T-cells...
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PMID: 10320367
PDF is available here.
PDF is available here.
Mammalian Genome (10)5 1999
Abstract
We have identified a cDNA that encodes a protein of 911 amino acids that contains two copies of the double-stranded RNA-binding motif and has 80% identity with human Interleukin Enhancer Binding Factor 3 (ILF3). Linkage and cytogenetic analyses localized the Ilf3 cDNA to a portion of mouse Chr 9, wh...
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PMID: 10337617
PDF is available here.
PDF is available here.
Biochemistry (Washington) (38)11 1999
A binding protein to the DNase I hypersensitive site II in HLA-DR alpha gene was identified as NF90.
Abstract
We previously observed that IFN gamma-inducible expression of the human MHC class II, HLA-DR alpha, gene was enhanced by treatment with 12-O-tetradecanoylphorbol-13-acetate (TPA) only in human monocytic leukemia THP-1 cells, but not in HeLa cells. In the HLA-DR alpha gene, three DNase I hypersensiti...
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PMID: 10079079
PDF is available here.
PDF is available here.
American Journal of Physiology -- Legacy Content (275)6 Pt 1 1998
Abstract
Human bronchial epithelial (HBE) cells express interleukin (IL)-2 [Y. Aoki, D. Qiu, A. Uyei, and P. N. Kao. Am. J. Physiol. 272 (Lung Cell. Mol. Physiol. 16): L276-L286, 1997]. 16HBE-transformed cells contain constitutive and inducible nuclear DNA-binding activity for the purine-box/nuclear factor (...
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PMID: 9843854
PDF is available here.
PDF is available here.
Abstract
We characterize and purify several human proteins that interact preferentially with VA RNAII in Northwestern blot assays. Two of these proteins were identified as RNA helicase A and NF90, a component of the heterodimeric nuclear factor of activated T cells (NFAT). They copurified with the smaller NF...
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PMID: 9671709
PDF is available here.
PDF is available here.
Abstract
We have recently assigned the gene encoding the small NFAT subunit, NF45 (ILF3) to human chromosome 1 (1q11-qter and 1p11-p12). This communication reports the assignment of the gene encoding the large NFAT subunit, NF90 or interleukin enhancer binding factor 3 gene (ILF3), to human chromosome 19 (19...
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PMID: 9620363
PDF is available here.
PDF is available here.
Abstract
We show, using electrophoretic mobility shift assays, that polypeptides in a fraction purified from human cells interact with DNA-PK and stabilize the formation of a complex containing DNA-PKcs-Ku and DNA. Five polypeptides in this fraction have been identified by amino-terminal sequence analysis an...
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PMID: 9442054
PDF is available here.
PDF is available here.
Abstract
Interleukin-2 (IL-2) is the first lymphokine secreted following T cell activation. Several transcription factors regulate IL-2 gene expression, including the nuclear factor of activated T cells (NFAT). NFAT acts at the antigen receptor response element-2 (ARRE-2) sequence in the IL-2 enhancer and is...
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PMID: 8974006
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (269)32 1994
Abstract
Nuclear Factor of Activated T-cells (NF-AT) is a crucial transcription factor required for T-cell expression of interleukin 2. Purified NF-AT contains 45-kDa and 90-kDa subunits (Corthésy, B., and Kao, P. N. (1994) J. Biol. Chem. 269, 20682-20690). Partial internal amino acid sequences derived from...
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PMID: 7519613
PDF is available here.
PDF is available here.