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Rho Factor (0):
Recent article
Rho Factor
Abstract
We report an efficient method to simultaneously measure multiple mRNA levels utilizing mass spectrometry (MS) and molecular affinity isolation. In this approach, reverse transcription products of a group of mRNAs are subjected to competitive PCR with competitors and internal standards of known conce...
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PMID: 20410241
PDF is available here.
PDF is available here.
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Methods in Molecular Biology (587)587 2010
Abstract
We describe complementary ATP-dependent RNA-DNA helicase and streptavidin displacement assays that can be used to monitor in vitro Rho's motor activity in a more direct and quantitative manner....
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PMID: 20225147
PDF is available here.
PDF is available here.
Abstract
PykF is one of two pyruvate kinases in Escherichia coli K-12. lambdaP(L) was convergently integrated into the chromosome of the MG1655 strain, downstream of pykF, face-to-face with its native promoter. In the presence of lambdacIts857, efficient pykF ts-silencing was achieved when the 5'-terminus of...
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PMID: 20068353
PDF is available here.
PDF is available here.
Abstract
We previously demonstrated that SP cells from teleost body kidney (BK) had the HSC activity by a transplantation experiment using clonal ginbuna crucian carp (Carassius auratus langsdorfii). In the present study, to isolate HSCs and hematopoietic progenitor cells (HPCs) from teleosts using Hoechst a...
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PMID: 18674821
PDF is available here.
PDF is available here.
Trends in Cell Biology (18)11 2008
Abstract
Some epithelia contain cells with multiple motile cilia that beat in a concerted manner. New tools and experimental systems have facilitated molecular studies of cilium biogenesis and the coordinated planar polarization of cilia that leads to their concerted motility. A recent elegant study using em...
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PMID: 18819800
PDF is available here.
PDF is available here.
Abstract
The study adds a fourth rhodopsin mutation associated with CSNB. Although the phenotype of autosomal dominant CSNB may vary slightly in patients showing mutations in RHO, PDE6B, or GNAT1, the disease course seems to be stationary with only scotopic vision being affected. The data indicate that the m...
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PMID: 18487375
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (283)29 2008
Abstract
We hypothesized that the conformational state(s) of the EC associated with this pausing would influence the action of Rho. Analyses of the pausing behavior of the EC at the termination points of two well known Rho-dependent terminators revealed that Rho prefers actively transcribing complexes for te...
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PMID: 18482981
PDF is available here.
PDF is available here.
Journal of Bacteriology (190)14 2008
Abstract
In Escherichia coli, interactions between the nascent TnaC-tRNA(Pro) peptidyl-tRNA and the translating ribosome create a tryptophan binding site in the ribosome where bound tryptophan inhibits TnaC-tRNA(Pro) cleavage. This inhibition delays ribosome release, thereby inhibiting Rho factor binding and...
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PMID: 18424524
PDF is available here.
PDF is available here.
Abstract
We used the antibiotic bicyclomycin, which inhibits Rho, to assess its role on a genome-wide scale. Rho is revealed as a global regulator of gene expression that matches Escherichia coli transcription to translational needs. We also found that genes in E. coli that are most repressed by Rho are prop...
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PMID: 18487194
PDF is available here.
PDF is available here.
Abstract
Rho guanine trinucleotide phosphatases are ubiquitious regulators of cytoskeletal structure and tension generation. Recent work shows that Rho-dependent mechanical interactions between cells and extracellular matrix regulate cell fate switching in capillary endothelial cells and megakaryocytes in vi...
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PMID: 18391790
PDF is available here.
PDF is available here.
Abstract
We report here that alpha-catulin also interacts with a key component of the NF-kappaB signalling pathway, namely the IkappaB kinase (IKK)-beta. In co-immunoprecipitations, alpha-catulin can bind IKK-beta and Lbc. Ectopic expression of alpha-catulin augmented NF-kappaB activity, promoted cell migrat...
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PMID: 17952117
PDF is available here.
PDF is available here.
Journal of Molecular Biology (377)4 2008
Abstract
We now report the cloning, expression, and purification of apo A-I derived from zebrafish (Danio rerio), which combines with phospholipids to form similar discoidal bilayers and may prove to be superior to human apo A-I constructs for rapid reconstitution of seven-transmembrane helix receptors into...
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PMID: 18313692
PDF is available here.
PDF is available here.
Abstract
C-peptide constricted afferent arterioles in diabetic mice by -27% compared with the control value. Normoglycemic arterioles administered C-peptide displayed a delayed and minute response (-4%). Scrambled C-peptide or vehicle administration, whether administered to hyperglycemic or normoglycemic mic...
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PMID: 18077505
PDF is available here.
PDF is available here.
Nucleic Acids Research (36)2 2008
Abstract
We identified two polycistronic tRNA transcripts whose endonucleolytic processing was solely dependent on RNase P. Here we show that the processing of the secG leuU and metT leuW glnU glnW metU glnV glnX polycistronic transcripts takes place through a different type of maturation pathway. Specifical...
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PMID: 18033800
PDF is available here.
PDF is available here.
Molecular Microbiology (67)1 2008
Abstract
We report experimental analysis of transcriptional terminators in the human pathogen Helicobacter pylori. Previous bioinformatics approaches came to differing conclusions regarding transcriptional termination in this bacterium. We used a reporter construct, the tnpR-encoded resolvase, to assess term...
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PMID: 18078442
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (282)43 2007
Abstract
We show that Rho is a molecular motor that can apply significant disruptive forces on heterologous nucleoprotein assemblies such as streptavidin bound to biotinylated RNA molecules. ATP-dependent disruption of the biotin-streptavidin interaction demonstrates that Rho is not mechanistically limited t...
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PMID: 17724015
PDF is available here.
PDF is available here.
Molecular Cell (27)6 2007
Abstract
We show that NusA is required for lambda-related phage 82 antiterminator Q(82) to construct a stable complex in which RNA-based termination mechanisms have restricted access to the emerging transcript; this result suggests a locale for both Q(82) and NusA near the beta flap domain of RNA polymerase....
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PMID: 17889665
PDF is available here.
PDF is available here.
Biochemistry (Washington) (46)33 2007
Abstract
We show that the Rho helicase can also elude a RNA structural block located far downstream from the single-stranded C-rich region but upstream from a reporter RNA-DNA hybrid. In this process, Rho hexamers do not melt the intervening RNA motif but require single-stranded RNA segments on both of its s...
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PMID: 17655325
PDF is available here.
PDF is available here.
Journal of Molecular Biology (371)4 2007
Abstract
We have isolated different termination defective mutants of Rho by random mutagenesis, characterized them for their different functions and established the structure-function correlations from the available structural data of Rho. These mutations are located within the two domains; the N-terminal RN...
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PMID: 17599352
PDF is available here.
PDF is available here.
Journal of Neuroscience (27)29 2007
Abstract
We show that minocycline improves functional recovery after SCI in part by reducing apoptosis of oligodendrocytes via inhibition of proNGF production in microglia. After SCI, the stress-responsive p38 mitogen-activated protein kinase (p38MAPK) was activated only in microglia, and proNGF was produced...
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PMID: 17634369
PDF is available here.
PDF is available here.
Abstract
This study was performed to determine the effectiveness of the Rho kinase inhibitor and NF-kappaB inhibitor in renal injury of ANG II-infused hypertensive rats. Male Sprague-Dawley rats, maintained on a normal diet, received either a sham operation (n = 7) or continuous ANG II infusion (120 ng/min)...
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PMID: 17409276
PDF is available here.
PDF is available here.
Current Biology (17)11 2007
Abstract
We report that ankyrin repeats domain protein 5 (xANR5), a novel FGF target gene product, regulates cell-protrusion formation and tissue separation, a process that develops the boundary between the ectoderm and mesoderm [2, 3], during Xenopus gastrulation. Loss of xANR5 function by antisense morphol...
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PMID: 17475493
PDF is available here.
PDF is available here.
Cryobiology (54)3 2007
Abstract
We supply in this communication evidence for the mutagenic effect of freezing on mitochondrial DNA (mtDNA) of the yeast Saccharomyces cerevisiae. The cooling for 2 h at +4 degrees C, followed by freezing for 1 h at -10 degrees C and 16 h at -20 degrees C resulted in induction of respiratory mutation...
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PMID: 17416359
PDF is available here.
PDF is available here.
Biochemical Journal (404)1 2007
Abstract
We show that Rho alone is not sufficient for this repulsion response. Rather, Cdc42 (cell division cycle 42) and its effector MRCK (myotonic dystrophy kinase-related Cdc42-binding kinase) are also critical for ephrinB-induced cell retraction. Stimulation of endothelial cells with ephrinB2 triggers r...
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PMID: 17300218
PDF is available here.
PDF is available here.
Journal of Endocrinology (192)3 2007
Abstract
We assessed these effects of fasudil in insulin-resistant diabetic rats, comparing them with those of an angiotensin II receptor blocker, olmesartan. Male Otsuka Long-Evans Tokushima fatty (OLETF) and Long-Evans Tokushima Otsuka, non-diabetic control, rats at 15 weeks of age were used. OLETF rats we...
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PMID: 17332527
PDF is available here.
PDF is available here.
Abstract
We hypothesize that FIZ1 is part of an interface between cell-specific factors, like NRL, and more ubiquitous regulatory networks that vary the absolute expression levels of some rod-specific genes (i.e. Rhodopsin). As part of an ongoing exploration of FIZ1's role in neural retina, in vivo, we have...
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PMID: 17141759
PDF is available here.
PDF is available here.
FEBS Letters (580)30 2006
Abstract
We investigated the distributions of termination motifs around the 3'-ends of overlapping and non-overlapping genes at the genomic level. We suggest that the positions of compactly encoded E. coli genes and rho-independent terminators are optimized to terminate the adjoining genes on their antisense...
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PMID: 17156778
PDF is available here.
PDF is available here.
Experimental Cell Research (312)20 2006
Abstract
Multidrug resistance (MDR) phenotype is characterized by the over-expression of P-glycoprotein (P-gp) on cell plasma membranes that extrudes several drugs out of cells. Cells that express the MDR phenotype are resistant to the mitochondrial related apoptosis and to several anticancer drugs. This stu...
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PMID: 17027968
PDF is available here.
PDF is available here.
Abstract
We have determined the structure of the Rho transcription termination factor, a hexameric RNA/DNA helicase, with single-stranded RNA bound to the motor domains of the protein. The structure reveals a closed-ring "trimer of dimers" conformation for the hexamer that contains an unanticipated arrangeme...
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PMID: 17081977
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (281)44 2006
Abstract
Rho factor is an essential protein that causes termination of transcription in a wide variety of bacteria by an RNA-dependent helicase activity. Rho is activated by transcripts that contain a high proportion of cytidine residues. The interaction between Rho and two adjacent cytidine residues within...
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PMID: 16908525
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (281)36 2006
Abstract
We propose that Psu inhibits Rho-dependent termination by slowing down the translocation of Rho along the RNA because of its slow ATPase activity....
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PMID: 16829521
PDF is available here.
PDF is available here.
Microbiology (152)Pt 9 2006
Abstract
Rho-dependent transcription terminators participate in sophisticated genetic regulatory mechanisms, in both bacteria and phages; they occur in regulatory regions preceding the coding sequences of genes and within coding sequences, as well as at the end of transcriptional units, to prevent readthroug...
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PMID: 16946247
PDF is available here.
PDF is available here.
Molecular Cell (22)6 2006
Abstract
Rho factor in bacteria terminates transcription by using energy from ATP hydrolysis to forcefully dissociate the transcripts from RNA polymerase. used data from presteady-state ATPase kinetics to support a rational mechanistic model for Rho's action on RNA.
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PMID: 16793540
PDF is available here.
PDF is available here.
Molecular Cell (22)5 2006
Abstract
We determine how Rho utilizes the chemical energy of ATP hydrolysis to translocate RNA. A random hydrolysis mechanism is ruled out by the observed inhibition of ATPase in a mixed hexamer containing wt and an inactive Rho mutant. We propose a mechanism in which (1) all six subunits are catalytically...
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PMID: 16762834
PDF is available here.
PDF is available here.
Journal of Microbiology (44)1 2006
Abstract
We have attempted to summarize "the knowns" and "the unknowns" of the Rho protein revealed by the recent developments in this field. An attempt has also been made to understand the physiology of Rho in the light of its phylogeny....
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PMID: 16554712
PDF is available here.
PDF is available here.
Journal of Molecular Biology (355)2 2006
Abstract
We report the first detailed in vitro investigation of archaeal transcription termination. Methanothermobacter thermautotrophicus (M.t.) RNAP is susceptible to intrinsic termination at an intergenic sequence that conforms to a bacterial intrinsic terminator, as well as at bona fide bacterial intrins...
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PMID: 16305799
PDF is available here.
PDF is available here.
Nucleic Acids Research (34)19 2006
Abstract
We demonstrate that >90% of E.coli open reading frames (ORFs) transcribed during exponential growth undergo some degree of polyadenylation by PAP I, either as full-length transcripts or decay intermediates. Detailed analysis of over 240 transcripts suggests that Rho-independent transcription termina...
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PMID: 17040898
PDF is available here.
PDF is available here.
Abstract
Rats infused with AngII for three days present renal inflammatory cell infiltration and slight tubular damage, which were diminished by treatment with the Rho-kinase inhibitor Y-27632. AngII activates nuclear factor-kappaB and causes overexpression of proinflammatory factors, including cytokines (tu...
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PMID: 16336575
PDF is available here.
PDF is available here.
Journal of Bacteriology (187)24 2005
Abstract
We show here that termination is Rho dependent and is suppressed in a rho mutant or by bicyclomycin treatment when fimE mRNA is expressed by the fimE gene, either from a multicopy recombinant plasmid or in its native chromosomal location. Two cis-acting elements within the central portion of the 314...
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PMID: 16321930
PDF is available here.
PDF is available here.
Cancer Letters (229)1 2005
Abstract
We will propose new avenues of research for interaction of the AGC kinase-PKG, with the Rho GTPases and Pak in the cell motility and cell morphology of endothelial cells....
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PMID: 16157214
PDF is available here.
PDF is available here.
Abstract
The small guanosine triphosphatase Rho and its target, Rho kinase, play important roles in both blood pressure regulation and vascular smooth muscle contraction. Rho is activated by agonists of receptors coupled to cell membrane G protein, such as angiotensin II and phenylephrine. Once Rho is activa...
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PMID: 16053829
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1729)2 2005
Abstract
We have tested the role of this appended mini-domain by assaying the elongation and termination enhancement activities of variants. The results show that variants with changes in their sequence do not cause a loss of functions, whereas variants with the deletions of the residues in that domain are m...
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PMID: 15890417
PDF is available here.
PDF is available here.
Abstract
The aims of the present investigation were to develop and test a sensitive and reproducible method for the study of gene expression in the porcine lung pathogen Actinobacillus pleuropneumoniae by real-time quantitative reverse transcription (RT)-PCR and to evaluate a number of suitable internal cont...
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PMID: 15932989
PDF is available here.
PDF is available here.
Journal of Bacteriology (187)12 2005
Abstract
The impossibility of obtaining a rho null mutant and sensitivity to bicyclomycin have indicated that rho is essential for the viability of Caulobacter crescentus. Transcription gene fusions of sequences with serial deletions of the rho 5' untranslated region (5'-UTR) with a lacZ reporter gene indica...
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PMID: 15937192
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1729)1 2005
Abstract
The Bacillus subtilis rho gene encodes the transcription termination factor Rho that is produced at a low level in B. subtilis cells. No typical Shine-Dalgarno (SD) sequence lies at an appropriate distance from the translational start site of rho. However, the nucleotide sequence GTGGTG present upst...
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PMID: 15866515
PDF is available here.
PDF is available here.
Genes to Cells (10)5 2005
Abstract
The role of transcription termination process for gene expression regulation is poorly understood. Either a multicopy supply of the rof gene or bicyclomycin, both of which inhibit the transcription termination Rho factor, suppressed the increased sensitivity to oxidative stress of the rifampicin-res...
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PMID: 15836776
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (280)14 2005
Abstract
Our results also show that there are kinetically slow branches of the enzyme mechanism that are not seen when the chemistry step of the catalytic cycle is fast. These branches become prominent, however, when two of the three Rho active sites are empty or bear non-hydrolyzable compounds. A first-orde...
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PMID: 15703178
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (280)14 2005
Abstract
Escherichia coli transcription termination factor Rho exhibits the phenomenon of catalytic cooperativity. The catalytic rate per site is 30-fold faster when all three sites are filled with substrate ATP than when only a single site is occupied (Stitt, B. L., and Xu, Y. (1998) J. Biol. Chem. 273, 264...
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PMID: 15703177
PDF is available here.
PDF is available here.
Journal of Bacteriology (187)8 2005
Abstract
We characterized the origins of the three transcripts and compared the relative expression of the BmpA and BmpB proteins. Northern blotting revealed that the three distinct transcripts originated from a single promoter located upstream of bmpA but terminated either 3' to the bmpA (1.1-kb RNA) or bmp...
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PMID: 15805505
PDF is available here.
PDF is available here.
Abstract
We report a novel technique using imine formation with rho to enhance the affinity of a bicyclomycin analogue and determine the binding stoichiometry by isothermal titration calorimetry (ITC) and mass spectrometry (MS). Our designed bicyclomycin ligand, 5a-(3-formyl-phenylsulfanyl)-dihydrobicyclomyc...
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PMID: 15725032
PDF is available here.
PDF is available here.
Nature Medicine (11)3 2005
Abstract
We identify Vav3, a Rho family guanine nucleotide exchange factor, as essential for stimulated osteoclast activation and bone density in vivo. Vav3-deficient osteoclasts show defective actin cytoskeleton organization, polarization, spreading and resorptive activity resulting from impaired signaling...
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PMID: 15711558
PDF is available here.
PDF is available here.
Abstract
We show that select bismuth-dithiol solutions inhibit the Escherichia coli rho transcription termination factor. Rho is an essential enzyme in most Gram-negative prokaryotes and without rho function the cells are not viable. Bismuth complexes with 2,3-dimercapto-1-propanol (BiBAL) (3:1 solutions) fu...
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PMID: 15708806
PDF is available here.
PDF is available here.
Abstract
We have determined three independent X-ray crystal structures of Rho complexed with BCM and two semisynthetic derivatives, 5a-(3-formylphenylsulfanyl)-dihydrobicyclomycin (FPDB) and 5a-formylbicyclomycin (FB) to 3.15, 3.05, and 3.15 A resolution, respectively. The structures show that BCM and its de...
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PMID: 15642265
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1742)1-3 2004
Abstract
We review the role of Rho and ARF GTPases in supplying actin and lipid structures required for synaptic vesicle and secretory granule exocytosis. Their possible functional interplay may provide the molecular cues for efficient and localized exocytotic fusion....
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PMID: 15590054
PDF is available here.
PDF is available here.
Molecular Pharmacology (66)6 2004
Abstract
We report that Galphaz signals inhibit serum response factor (SRF)-dependent transcription. Galphaz expression inhibits Galpha12/13-, Galphaq-, and Rho guanine nucleotide exchange factor (GEF)-induced serum response element (SRE) reporter activation in human embryonic kidney 293T and PC-12 cells. Ex...
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PMID: 15326221
PDF is available here.
PDF is available here.
Journal of Molecular Biology (342)2 2004
Abstract
We have designed a collection of Rho substrate chimeras containing an RNA-DNA helix located at various positions with respect to a short (47 nucleotides) artificial loading site. We show that these synthetic constructs represent interesting model substrates able to engage in a productive interaction...
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PMID: 15327943
PDF is available here.
PDF is available here.
Journal of Molecular Biology (339)5 2004
Abstract
We show that the intervening boxB RNA motif has a double function in the mechanisms of termination/antitermination at lambdatR1. As a folded hairpin structure, it acts as a clamp that holds rutA and rutB side by side for optimal interactions with Rho leading to efficient termination. Conversely, the...
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PMID: 15178249
PDF is available here.
PDF is available here.
Development (131)12 2004
Abstract
We analyzed Rho GTPases that could direct axon outgrowth and/or nuclear migration. We show that the expression pattern of Rho GTPases in developing PCN is consistent with their involvement in the migration of PCN from the rhombic lips. We report that pharmacological inhibition of Rho enhances axon o...
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PMID: 15151987
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (279)18 2004
Abstract
We have discerned the conformational changes in the Rho protein that occur upon nucleotide and nucleic acid binding. We show that the 2', (3')-O-[N-methylanthraniloyl] derivative of ATP (mant-ATP) is a good fluorescent substrate of Rho and is hydrolyzed with a K(m) comparable with that for ATP but a...
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PMID: 14970217
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (279)16 2004
Abstract
The binding of C10 RNA oligomers to wild type and mutant Escherichia coli transcription termination factor Rho provides a model for the enzyme-RNA interactions that lead to transcription termination. One surprising finding is that wild type Rho binds between five and six C10 oligomers per hexamer wi...
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PMID: 14761943
PDF is available here.
PDF is available here.