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Viral Nonstructural Proteins (0):
Recent article
Viral Nonstructural Proteins
Abstract
Hepatitis C virus (HCV) nonstructural protein 5A (NS5A) has been shown to modulate multiple cellular processes, including apoptosis. The aim of this study was to assess the effects of HCV NS5A on apoptosis induced by Toll-like receptor (TLR) 4 ligand, lipopolysaccharide (LPS)....
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PMID: 21844306
PDF is available here.
PDF is available here.
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Xenobiotica (41)5 2011
Abstract
We recently showed that isolated replication complexes are able to synthesize two species of nascent viral RNA, one double stranded and the other single stranded. NS5B nucleoside inhibitors block synthesis of both species, whereas nonnucleoside inhibitors inhibit mostly single- tranded RNA synthesis...
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PMID: 19009261
PDF is available here.
PDF is available here.
Abstract
These results suggest that the rotavirus VP6, NSP1 and NSP5 genes of Wa-like rotaviruses are more prone to temporal mutations. Both structural and nonstructural genes of the Western Indian rotavirus strains shared nucleotide and amino acid substitutions with the Bangladeshi strain, Dhaka16-03 (G1P[8...
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PMID: 21256248
PDF is available here.
PDF is available here.
Abstract
We focused our strategy on the allosteric inhibitors capable of targeting the NS2B-NS3pro interface rather than the NS3pro active site. Using virtual ligand screening of the diverse, ∼275,000-compound library and the catalytic domain of the two-component West Nile virus (WNV) NS2B-NS3pro as a rece...
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PMID: 21050032
PDF is available here.
PDF is available here.
Abstract
Structure-based library design employs both structure-based drug design (SBDD) and combinatorial library design. Combinatorial library design concepts have evolved over the past decade, and this chapter covers several novel aspects of structure-based library design together with successful case stud...
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PMID: 20981524
PDF is available here.
PDF is available here.
Abstract
We analyzed the incidence of BA, morphological change, morphogenesis of viral particles and viral mRNA and protein expression. The in vitro experiments showed NSP4 silencing decreased the levels of VP7 and VP4, reduced viral particles and decreased cytopathic effect. NSP4-positive cells had strongly...
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PMID: 21876759
PDF is available here.
PDF is available here.
Abstract
We analyze all available RBD, ED, and full-length NS1 structures, including four novel crystal structures obtained using EDs from divergent human and avian viruses, as well as two forms of a monomeric ED mutant. The data reveal the helix-helix interface as the only strictly conserved ED homodimeric...
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PMID: 21464929
PDF is available here.
PDF is available here.
Virology Journal (8)1 2011
Abstract
We analyse the effect of these differences upon the binding of Influenza A virus NS1 protein to a range of cellular proteins involved in polarity and signal transduction....
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PMID: 21247458
PDF is available here.
PDF is available here.
Abstract
We showed that the nuclear, mature SREBP-1c level increases in the nucleus of replicon cells expressing HCV-3a nonstructural protein-5A (NS5A). We further showed that HCV-3a NS5A up-regulates SREBP-1c transcription. Additional analysis showed that transcriptional factor Sp1 is involved in SREBP-1c a...
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PMID: 20971080
PDF is available here.
PDF is available here.
Abstract
These results illustrate the great progress that has been made in the HCV field and how this knowledge can be used to devise innovative strategies to counteract this pathogen.
© Thieme Medical Publishers....
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PMID: 20960374
PDF is available here.
PDF is available here.
Abstract
We developed a method to recover recombinant viruses in which independent selection strategies are used to engineer single-gene replacements. We coupled a mutant SA11 RV encoding a temperature-sensitive (ts) defect in the NSP2 protein with RNAi-mediated degradation of NSP2 mRNAs to isolate a virus c...
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PMID: 20937889
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (285)43 2010
Abstract
We describe a combined structural and functional analysis of genotype 1 HCV-NS5b of strains H77 (subtype 1a), for which no structure has been previously reported, and J4 (subtype 1b). Our results highlight the linker as directly involved in lifting the first boundary to processive RNA synthesis, the...
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PMID: 20729191
PDF is available here.
PDF is available here.
Abstract
I strains from the 2000s was noteworthy (4.7-6.7%). Sequencing and phylogenetic analysis of the NSP4 genes showed almost equal distribution (45.0-55.0%) of genotypes A and B however, higher amino acid divergence within the genotype B strains (up to 9.3%) than in genotype A strains (up to 2.9%) at th...
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PMID: 20542145
PDF is available here.
PDF is available here.
Abstract
The A Iran 05 foot-and-mouth disease virus (FMDV) subtype was detected in Iran during 2005 and has proven to be highly virulent. This study was undertaken to focus on molecular and phylogenetic analysis of 3A and 3B coding-regions in the A Iran 05 field isolate. To assess the genetic relatedness of...
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PMID: 20706032
PDF is available here.
PDF is available here.
Abstract
We review our current molecular knowledge of transmissibility and pathogenicity of influenza viruses and discuss the future aspects of the pandemic virus....
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PMID: 20845737
PDF is available here.
PDF is available here.
Abstract
We have reported that specifically designed SRPK inhibitors suppressed effectively several DNA and RNA viruses in vitro and in vivo. Here, we show that an SRPK inhibitor, SRPIN340, suppressed in a dose-dependent fashion expression of a hepatitis C virus (HCV) subgenomic replicon and replication of t...
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PMID: 20498328
PDF is available here.
PDF is available here.
Abstract
The hepatitis C virus (HCV) NS5B RNA polymerase facilitates the RNA synthesis step during the HCV replication cycle. Nucleoside analogs targeting the NS5B provide an attractive approach to treating HCV infections because of their high barrier to resistance and pan-genotype activity. PSI-7851, a pron...
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PMID: 20516278
PDF is available here.
PDF is available here.
Virus Research (151)2 2010
Abstract
We survey biochemical and recent structural investigations on NS2 important to the understanding of the molecular events underlying the process of BTV morphogenesis. We also present a phylogenetic analysis of the NS2 sequences....
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PMID: 20621672
PDF is available here.
PDF is available here.
Virus Research (151)2 2010
Abstract
We found that NS1 genes originating from two H3 avian influenza viruses, A/duck/Beijing/40/04 (Dk/BJ/40/04) and A/duck/Beijing/61/05 (Dk/BJ/61/05), possessing three amino acid residue differences at positions 127, 205 and 209 contributed to an altered virulence in rescued NS1 recombinant viruses on...
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PMID: 20546807
PDF is available here.
PDF is available here.
Virus Research (151)2 2010
Abstract
We have examined L4-22K and L4-33K for possible DEF-A activity. We show that L4-22K stimulates transcription from the MLP in a DE sequence dependent manner both in vivo and in vitro, and that L4-22K binds to the DE sequence in vitro. Further, the position of the L4-22K DNA binding site in a promoter...
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PMID: 20621673
PDF is available here.
PDF is available here.
Journal of General Virology (91)Pt 8 2010
Abstract
Bunyamwera virus NSs protein is involved in the inhibition of cellular transcription and the interferon (IFN) response, and it interacts with the Med8 component of Mediator. A spontaneous mutant of a recombinant NSs-deleted Bunyamwera virus (rBUNdelNSs2) was identified and characterized. This mutant...
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PMID: 20427562
PDF is available here.
PDF is available here.
Journal of General Virology (91)Pt 8 2010
Abstract
We conducted a yeast two-hybrid screening. As a result, we identified a cellular protein termed bovine NIK- and IKKbeta-binding protein (NIBP), which is involved in protein trafficking and nuclear factor kappa B (NF-kappaB) signalling in cells. The interaction of NS5A with NIBP was confirmed both in...
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PMID: 20444997
PDF is available here.
PDF is available here.
Journal of Virology (84)16 2010
Abstract
We employed a lentivirus-based RNA interference (RNAi) screening approach to search for possible cellular factors. By using a kinase-phosphatase RNAi library and an HCV replicon reporter system, we identified a serine-threonine kinase, Polo-like kinase 1 (Plk1), as a potential host factor regulating...
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PMID: 20534861
PDF is available here.
PDF is available here.
Journal of General Virology (91)Pt 8 2010
Abstract
Our study also demonstrated that expression of H1N1/09 NS1 resulted in enhanced replication of rNDV in human cells, indicating that function of the NS1 proteins can be host-species-specific....
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PMID: 20410312
PDF is available here.
PDF is available here.
Journal of Molecular Biology (400)4 2010
Abstract
Nonstructural protein 3 of the severe acute respiratory syndrome (SARS) coronavirus includes a "SARS-unique domain" (SUD) consisting of three globular domains separated by short linker peptide segments. This work reports NMR structure determinations of the C-terminal domain (SUD-C) and a two-domain...
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PMID: 20493876
PDF is available here.
PDF is available here.
Journal of Molecular Biology (400)3 2010
Abstract
We show here that both activation and inhibition by full-length IRES are possible. The HCV IRES has a complex secondary structure comprising four domains. While it has been demonstrated that domains III-IV activate PKR, we report here that domain II of the IRES also potently activates. Structure map...
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PMID: 20447405
PDF is available here.
PDF is available here.
Abstract
In the past decade, intensive efforts have focused on the discovery of both nucleos(t)ide and non-nucleoside inhibitors of the HCV NS5B polymerase. These efforts have resulted in several promising agents advancing in clinical development. This review traces the history of optimization of the chemica...
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PMID: 20597029
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
We have predicted a putative basic leucine zipper (bZIP) motif within the aminoterminal part of NS4B. The aim of this study was to investigate the importance of this NS4B bZIP motif for specific protein-protein interactions. We applied in silico approaches for 3D-structure modeling of NS4B-homodimer...
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PMID: 20506268
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
We have predicted a putative basic leucine zipper (bZIP) motif within the aminoterminal part of NS4B. The aim of this study was to investigate the importance of this NS4B bZIP motif for specific protein-protein interactions. We applied in silico approaches for 3D-structure modeling of NS4B-homodimer...
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PMID: 20506268
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
We have predicted a putative basic leucine zipper (bZIP) motif within the aminoterminal part of NS4B. The aim of this study was to investigate the importance of this NS4B bZIP motif for specific protein-protein interactions. We applied in silico approaches for 3D-structure modeling of NS4B-homodimer...
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PMID: 20506268
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
We have predicted a putative basic leucine zipper (bZIP) motif within the aminoterminal part of NS4B. The aim of this study was to investigate the importance of this NS4B bZIP motif for specific protein-protein interactions. We applied in silico approaches for 3D-structure modeling of NS4B-homodimer...
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PMID: 20506268
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
We have predicted a putative basic leucine zipper (bZIP) motif within the aminoterminal part of NS4B. The aim of this study was to investigate the importance of this NS4B bZIP motif for specific protein-protein interactions. We applied in silico approaches for 3D-structure modeling of NS4B-homodimer...
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PMID: 20506268
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
We have predicted a putative basic leucine zipper (bZIP) motif within the aminoterminal part of NS4B. The aim of this study was to investigate the importance of this NS4B bZIP motif for specific protein-protein interactions. We applied in silico approaches for 3D-structure modeling of NS4B-homodimer...
|
PMID: 20506268
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
We have predicted a putative basic leucine zipper (bZIP) motif within the aminoterminal part of NS4B. The aim of this study was to investigate the importance of this NS4B bZIP motif for specific protein-protein interactions. We applied in silico approaches for 3D-structure modeling of NS4B-homodimer...
|
PMID: 20506268
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
We have predicted a putative basic leucine zipper (bZIP) motif within the aminoterminal part of NS4B. The aim of this study was to investigate the importance of this NS4B bZIP motif for specific protein-protein interactions. We applied in silico approaches for 3D-structure modeling of NS4B-homodimer...
|
PMID: 20506268
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
We have predicted a putative basic leucine zipper (bZIP) motif within the aminoterminal part of NS4B. The aim of this study was to investigate the importance of this NS4B bZIP motif for specific protein-protein interactions. We applied in silico approaches for 3D-structure modeling of NS4B-homodimer...
|
PMID: 20506268
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
We have predicted a putative basic leucine zipper (bZIP) motif within the aminoterminal part of NS4B. The aim of this study was to investigate the importance of this NS4B bZIP motif for specific protein-protein interactions. We applied in silico approaches for 3D-structure modeling of NS4B-homodimer...
|
PMID: 20506268
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
We have predicted a putative basic leucine zipper (bZIP) motif within the aminoterminal part of NS4B. The aim of this study was to investigate the importance of this NS4B bZIP motif for specific protein-protein interactions. We applied in silico approaches for 3D-structure modeling of NS4B-homodimer...
|
PMID: 20506268
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
We have predicted a putative basic leucine zipper (bZIP) motif within the aminoterminal part of NS4B. The aim of this study was to investigate the importance of this NS4B bZIP motif for specific protein-protein interactions. We applied in silico approaches for 3D-structure modeling of NS4B-homodimer...
|
PMID: 20506268
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
We have predicted a putative basic leucine zipper (bZIP) motif within the aminoterminal part of NS4B. The aim of this study was to investigate the importance of this NS4B bZIP motif for specific protein-protein interactions. We applied in silico approaches for 3D-structure modeling of NS4B-homodimer...
|
PMID: 20506268
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
We have predicted a putative basic leucine zipper (bZIP) motif within the aminoterminal part of NS4B. The aim of this study was to investigate the importance of this NS4B bZIP motif for specific protein-protein interactions. We applied in silico approaches for 3D-structure modeling of NS4B-homodimer...
|
PMID: 20506268
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
We have predicted a putative basic leucine zipper (bZIP) motif within the aminoterminal part of NS4B. The aim of this study was to investigate the importance of this NS4B bZIP motif for specific protein-protein interactions. We applied in silico approaches for 3D-structure modeling of NS4B-homodimer...
|
PMID: 20506268
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
We have predicted a putative basic leucine zipper (bZIP) motif within the aminoterminal part of NS4B. The aim of this study was to investigate the importance of this NS4B bZIP motif for specific protein-protein interactions. We applied in silico approaches for 3D-structure modeling of NS4B-homodimer...
|
PMID: 20506268
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
We have predicted a putative basic leucine zipper (bZIP) motif within the aminoterminal part of NS4B. The aim of this study was to investigate the importance of this NS4B bZIP motif for specific protein-protein interactions. We applied in silico approaches for 3D-structure modeling of NS4B-homodimer...
|
PMID: 20506268
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
We have predicted a putative basic leucine zipper (bZIP) motif within the aminoterminal part of NS4B. The aim of this study was to investigate the importance of this NS4B bZIP motif for specific protein-protein interactions. We applied in silico approaches for 3D-structure modeling of NS4B-homodimer...
|
PMID: 20506268
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
We have predicted a putative basic leucine zipper (bZIP) motif within the aminoterminal part of NS4B. The aim of this study was to investigate the importance of this NS4B bZIP motif for specific protein-protein interactions. We applied in silico approaches for 3D-structure modeling of NS4B-homodimer...
|
PMID: 20506268
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
We have predicted a putative basic leucine zipper (bZIP) motif within the aminoterminal part of NS4B. The aim of this study was to investigate the importance of this NS4B bZIP motif for specific protein-protein interactions. We applied in silico approaches for 3D-structure modeling of NS4B-homodimer...
|
PMID: 20506268
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
We have predicted a putative basic leucine zipper (bZIP) motif within the aminoterminal part of NS4B. The aim of this study was to investigate the importance of this NS4B bZIP motif for specific protein-protein interactions. We applied in silico approaches for 3D-structure modeling of NS4B-homodimer...
|
PMID: 20506268
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
We have predicted a putative basic leucine zipper (bZIP) motif within the aminoterminal part of NS4B. The aim of this study was to investigate the importance of this NS4B bZIP motif for specific protein-protein interactions. We applied in silico approaches for 3D-structure modeling of NS4B-homodimer...
|
PMID: 20506268
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
We have predicted a putative basic leucine zipper (bZIP) motif within the aminoterminal part of NS4B. The aim of this study was to investigate the importance of this NS4B bZIP motif for specific protein-protein interactions. We applied in silico approaches for 3D-structure modeling of NS4B-homodimer...
|
PMID: 20506268
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
We have predicted a putative basic leucine zipper (bZIP) motif within the aminoterminal part of NS4B. The aim of this study was to investigate the importance of this NS4B bZIP motif for specific protein-protein interactions. We applied in silico approaches for 3D-structure modeling of NS4B-homodimer...
|
PMID: 20506268
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
We have predicted a putative basic leucine zipper (bZIP) motif within the aminoterminal part of NS4B. The aim of this study was to investigate the importance of this NS4B bZIP motif for specific protein-protein interactions. We applied in silico approaches for 3D-structure modeling of NS4B-homodimer...
|
PMID: 20506268
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
We have predicted a putative basic leucine zipper (bZIP) motif within the aminoterminal part of NS4B. The aim of this study was to investigate the importance of this NS4B bZIP motif for specific protein-protein interactions. We applied in silico approaches for 3D-structure modeling of NS4B-homodimer...
|
PMID: 20506268
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
We have predicted a putative basic leucine zipper (bZIP) motif within the aminoterminal part of NS4B. The aim of this study was to investigate the importance of this NS4B bZIP motif for specific protein-protein interactions. We applied in silico approaches for 3D-structure modeling of NS4B-homodimer...
|
PMID: 20506268
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
We have predicted a putative basic leucine zipper (bZIP) motif within the aminoterminal part of NS4B. The aim of this study was to investigate the importance of this NS4B bZIP motif for specific protein-protein interactions. We applied in silico approaches for 3D-structure modeling of NS4B-homodimer...
|
PMID: 20506268
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
We have predicted a putative basic leucine zipper (bZIP) motif within the aminoterminal part of NS4B. The aim of this study was to investigate the importance of this NS4B bZIP motif for specific protein-protein interactions. We applied in silico approaches for 3D-structure modeling of NS4B-homodimer...
|
PMID: 20506268
PDF is available here.
PDF is available here.
Protein Science (19)7 2010
Abstract
We have predicted a putative basic leucine zipper (bZIP) motif within the aminoterminal part of NS4B. The aim of this study was to investigate the importance of this NS4B bZIP motif for specific protein-protein interactions. We applied in silico approaches for 3D-structure modeling of NS4B-homodimer...
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PMID: 20506268
PDF is available here.
PDF is available here.
Journal of Virology (84)13 2010
Abstract
Rotaviruses are a major cause of acute gastroenteritis in children worldwide. Early stages of rotavirus assembly in infected cells occur in viroplasms. Confocal microscopy demonstrated that viroplasms associate with lipids and proteins (perilipin A, ADRP) characteristic of lipid droplets (LDs). LD-a...
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PMID: 20335253
PDF is available here.
PDF is available here.
Journal of Virology (84)13 2010
Abstract
We describe the homodimeric crystal structure of PRRSV nsp1beta in its natural, self-processed form. We show that the architecture of its N-terminal domain (NTD) adopts a fold closely resembling that of several known nucleases and has intrinsic nuclease activity that is strongly activated by mangane...
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PMID: 20410261
PDF is available here.
PDF is available here.
Journal of Virology (84)13 2010
Abstract
We generated a wild-type H7N1 virus with an ESEV motif and a mutant virus with an NS1 protein containing a C-terminal RSKV motif by reverse genetics. We compared the phenotypes of these viruses in vitro in human, mouse, and duck cells as well as in vivo in mice and ducks. In human cells, the human C...
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PMID: 20410267
PDF is available here.
PDF is available here.