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Immediate-Early Proteins (4):
Recent article
Immediate-Early Proteins
Ugeskrift for laeger (173)2 2011
Abstract
Recent studies have shown expression of the following regulatory WNK kinases in the kidney: the full-length WNK1 (L-WNK1), the shorter kidney specific WNK1 transcript (KS-WNK1), formed by alternative splicing, and WNK4. Aldosterone activates expression of KS-WNK1 and inhibits WNK4 via SGK1 - both le...
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PMID: 21219845
PDF is available here.
PDF is available here.
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Abstract
We examined the effects of nominally selective inhibitors of PI3K (wortmannin, PI103, GDC-0941), SGK1 (GSK650394A) and PKB (Akti-1/2) on Na(+) transport in hormone-deprived and insulin-stimulated cortical collecting duct (mpkCCD) cells, while PI3K, SGK1 and PKB activities were assayed by monitoring...
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PMID: 20880397
PDF is available here.
PDF is available here.
Hypertension Research (33)10 2010
Abstract
This study was designed to test whether altered aldosterone-related sodium handling leads to salt-sensitive blood pressure in diabetes and thus may exaggerate end-organ damage. Zucker diabetic fatty (ZDF) rats, a model of type 2 diabetes, and Zucker lean (ZL) rats, as euglycemic controls, were divid...
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PMID: 20664544
PDF is available here.
PDF is available here.
Abstract
To investigate the effect of RNA interference targeting human cytomegalovirus immediate early gene 1 (HCMV- IE1) on the gene expression in vitro.
According to the sequence of HCMV-IE1 gene, the small interfering RNA (siRNA) sequences were designed and introduced into...
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PMID: 20855254
PDF is available here.
PDF is available here.
Abstract
To investigate the effect of RNA interference targeting human cytomegalovirus immediate early gene 1 (HCMV- IE1) on the gene expression in vitro.
According to the sequence of HCMV-IE1 gene, the small interfering RNA (siRNA) sequences were designed and introduced into...
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PMID: 20855254
PDF is available here.
PDF is available here.
Abstract
I (SGK1) reversed the inhibitory effect of WNK4 on ROMK through the phosphorylation of WNK4. However, Src-family protein tyrosine kinase (SFK) abolished the effect of SGK1 on WNK4 and restored the WNK4-induced inhibition of ROMK.
Basolateral K channels including big-conductance K channel and Kir4.1/...
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PMID: 20601877
PDF is available here.
PDF is available here.
Abstract
To investigate the effect of dracorhodin perchlorate (DP) on inhibiting high glucose-induced serum and glucocorticoid induced protein kinase 1 (SGK1) and fibronectin (FN) expression in human mesangial cells (HMC), and its mechanism of prevention and treatment on renal fibrosis in diabetic nephropathy...
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PMID: 20931854
PDF is available here.
PDF is available here.
Abstract
We show that VP22 physically interacted with infected cell polypeptide 0 (ICP0) and colocalized in the nucleus, indicating that VP22 could be functionally involved in the modulation of viral transcription through interaction with ICP0. In the HSV-1 infection system and chloramphenicol acetyltransfer...
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PMID: 20457214
PDF is available here.
PDF is available here.
Journal of Virology (84)16 2010
Abstract
We showed that SUMO binding by IE2 is necessary for its efficient transactivation function and for viral growth. IE2 bound physically to SUMO-1 through a SUMO-interacting motif (SIM). Mutations in SIM (mSIM) or in both SUMOylation sites and SIM (KR/mSIM), significantly reduced IE2 transactivation ef...
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PMID: 20519406
PDF is available here.
PDF is available here.
Journal of Virology (84)16 2010
Abstract
We isolated and characterized a spontaneously occurring MCMV mutant that has gained the capacity to replicate rapidly and to high titers in human cells. Compared to the parental wild-type (wt) virus, this mutant formed larger nuclear replication compartments and replicated viral DNA more efficiently...
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PMID: 20519391
PDF is available here.
PDF is available here.
Abstract
We have previously reported that Dot1a is located in the cytoplasm and nucleus (Reisenauer MR, Anderson M, Huang L, Zhang Z, Zhou Q, Kone BC, Morris AP, Lesage GD, Dryer SE, Zhang W. J Biol Chem 284: 35659-35669, 2009), widely expressed in the kidney as detected by its histone H3K79 methyltransferas...
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PMID: 20427473
PDF is available here.
PDF is available here.
Journal of Virology (84)13 2010
Abstract
We demonstrate that disruption of the ORF61p RING finger domain by amino acid substitution (Cys19Gly) alters ORF61p intranuclear distribution and abolishes ORF61p-mediated dispersion of Sp100-containing nuclear bodies. In addition, we demonstrate that an intact ORF61p RING finger domain is necessary...
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PMID: 20392849
PDF is available here.
PDF is available here.
Journal of Virology (84)13 2010
Abstract
We performed in vitro binding assays with N-terminal proteins encoding amino acids 1 to 160 of wild-type ICP27 or arginine-to-lysine substitution mutants. The R138,148,150K triple mutant bound weakly to sequences that were bound by the wild-type protein and single and double mutants. Furthermore, du...
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PMID: 20410270
PDF is available here.
PDF is available here.
Journal of Virology (84)13 2010
Abstract
We found that widespread mitochondrial distribution occurs within 1 h of pUL37x1 synthesis. The pUL37x1 mitochondrial targeting signal (MTS) spans its first antiapoptotic domain (residues 5 to 34) and consists of a weak hydrophobicity leader (MTSalpha) and proximal downstream residues (MTSbeta). Thi...
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PMID: 20410282
PDF is available here.
PDF is available here.
Veterinary Microbiology (143)1 2010
Abstract
We introduced the BICP0-5 frameshift into the BoHV-1 genome cloned as a bacterial artificial chromosome (BoHV-1 BAC) using the Red recombination system with galK selection and counterselection. Transfection of MDBK cells with the resulting BAC produced recombinant virus that replicated like wild typ...
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PMID: 20211531
PDF is available here.
PDF is available here.
Abstract
We have recently reported that N-myc downstream-regulated gene 1 (NDRG1)/Ca(2+)-associated protein with a molecular mass of 43kDa (Cap43) suppresses angiogenesis and tumor growth of pancreatic cancer through marked decreases in both the expression of CXC chemokines and phosphorylation of a NF-kappaB...
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PMID: 20416281
PDF is available here.
PDF is available here.
Abstract
We report here that the PLD/mTOR-dependent stabilization of HDM2 involves mTORC2 and the AGC family kinase serum- and glucocorticoid-inducible kinase 1 (SGK1). This study reveals that mTORC2 is a critical target of PLD-mediated survival signals and identifies SGK1 as a downstream target of mTORC2 fo...
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PMID: 20438709
PDF is available here.
PDF is available here.
Abstract
We investigated the nature of its specific anti-HCMV activity. Structure-activity relationship studies on a small series of analogues showed that WC5 possesses the most suitable pattern of substitutions around the quinolone scaffold to give potent and selective anti-HCMV activity. Studies performed...
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PMID: 20194695
PDF is available here.
PDF is available here.
Bing du xue bao = Chinese journal of virology / [bian ji, Bing du xue bao bian ji wei yuan hui] (26)3 2010
Abstract
We conclude that siRNA targeting ICP4 attenuates HSV-1 replication. Further more, multi-site siRNAs show stronger inhibitory effect on viral replication, which may be an effective and feasible approach for biological anti-viral drugs....
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PMID: 20572335
PDF is available here.
PDF is available here.
Journal of General Virology (91)Pt 5 2010
Abstract
Efficient replication of varicella-zoster virus (VZV) in cell culture requires expression of protein encoded by VZV open reading frame 63 (ORF63p). Two-dimensional gel analysis demonstrates that ORF63p is extensively modified. Mass spectroscopy analysis of ORF63p isolated from transiently transfecte...
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PMID: 20089801
PDF is available here.
PDF is available here.
Abstract
Genetic association analysis found a strong statistically significant correlation with the LTNP phenotype (single-nucleotide polymorphism rs1048412; P = .0004), independently of HLA-A10 influence. siRNA-based functional analysis showed that ZNRD1 down-regulation by siRNA or shRNA impaired HIV-1 repl...
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PMID: 20192730
PDF is available here.
PDF is available here.
Hypertension Research (33)3 2010
Abstract
Cilostazol (CILO), a selective inhibitor of phosphodiesterase 3 with potent antithrombotic property, has been shown to have a vasculoprotective effect in atherosclerosis animal models due to its potential anti-inflammatory and antioxidant actions. This study was undertaken to investigate whether CIL...
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PMID: 20019701
PDF is available here.
PDF is available here.
Antiviral Research (85)3 2010
Abstract
Our immunoblotting and flow cytometry analyses find that moronic acid, found in galls of Rhus chinensis and Brazilian propolis, at 10microM inhibits the expression of Rta, Zta, and an EBV early protein, EA-D, after lytic induction with sodium butyrate. This study also finds that moronic acids inhibi...
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PMID: 19969023
PDF is available here.
PDF is available here.
Abstract
As one of the immediate-early (IE) proteins of herpes simplex virus type 1 (HSV-1), ICP22 is a multifunctional viral regulator that localizes in the nucleus of infected cells. It is required in experimental animal systems and some nonhuman cell lines, but not in Vero or HEp-2 cells. ICP22 is extensi...
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PMID: 20960278
PDF is available here.
PDF is available here.
Abstract
Our results of increased expression of SGK1 in untreated CD may suggest its contribution to the enterocyte survival in this disease....
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PMID: 19966577
PDF is available here.
PDF is available here.
Journal of Virology (84)3 2010
Abstract
Herpes simplex virus (HSV) immediate-early (IE) protein ICP0 is a multifunctional regulator of HSV infection. ICP0 that is present in the tegument layer has not been well characterized. Protein compositions of wild-type and ICP0 null virions were similar, suggesting that the absence of ICP0 does not...
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PMID: 19906912
PDF is available here.
PDF is available here.
Journal of Virology (84)4 2010
Abstract
We identified transducin-like enhancer of split 2 (TLE2) as a novel RTA binding protein by using yeast two-hybrid screening of a human spleen cDNA library. The interaction between TLE2 and RTA was confirmed by glutathione S-transferase (GST) binding and coimmunoprecipitation assays. Immunofluorescen...
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PMID: 19939918
PDF is available here.
PDF is available here.
Journal of Virology (84)3 2010
Abstract
We therefore used chromatin immunoprecipitation to determine whether U2OS cells are competent to load histones onto HSV DNA and, if so, whether ICP0 and/or VP16 are required to reduce histone occupancy and enhance acetylation in this cell type. High levels of underacetylated histone H3 accumulated a...
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PMID: 19939931
PDF is available here.
PDF is available here.
Journal of Virology (84)4 2010
Abstract
We identified mitogen-activated protein kinase kinase kinase 8 (MAP3K8/Tpl2) as a positive regulator of murine gammaherpesvirus 68 (MHV-68 or gammaHV-68) lytic gene expression and replication. Tpl2 enhances MHV-68 lytic replication by upregulating lytic gene expression and promoter activities of vir...
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PMID: 19939924
PDF is available here.
PDF is available here.
Journal of Virology (84)3 2010
Abstract
We hypothesized that the intrathecal production of antibody to IE62 cross-reacting with BDNF and the nerve injury by skin lesions may augment allodynia in zoster by enhancing BDNF activity. One of three monoclonal antibodies against the 268-556 peptide of IE62 recognized BDNF. Immunological cross-re...
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PMID: 19923188
PDF is available here.
PDF is available here.
Acta Virologica (54)2 2010
Abstract
We analyzed the initiation of DNA replication in HCMV-permissive U373MG cells and used oriP of Epstein-Barr virus (EBV) as a simplified model of a cellular replication origin. Using U373MG cells we found that HCMV IE86 protein was bound to Mcm3, but did not inhibit the cellular DNA synthesis. Using...
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PMID: 20545442
PDF is available here.
PDF is available here.
Abstract
25 SNPs in the SCNN1A, SCNN1B, SCNN1G, NEDD4L, NDFIP2, and USP2 loci were found to be associated with blood pressure. An additional hypertension case-control study identified 13 SNPs in SCNN1B, SCNN1G, and NEDD4L that were linked to hypertension. CONCLUSION: These results support our hypothesis that...
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PMID: 20090362
PDF is available here.
PDF is available here.
Abstract
The varicella-zoster virus (VZV) open reading frame (ORF) 66 encodes a basophilic kinase orthologous to the US3 protein kinases found in all alphaherpesviruses. This review summarizes current information on the ORF66 kinase, and outlines apparent differences from other US3 kinases, as well as some o...
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PMID: 20186610
PDF is available here.
PDF is available here.
Abstract
To develop a gene therapeutic method for human cytomegalovirus (HCMV), the IE86 specific short hairpin (sh) RNA expressing vector was constructed and subsequently transfected into MRC-5 cells. After infection of these cells with HCMV AD169, expression of IE86 was reduced strikingly as compared to th...
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PMID: 20622455
PDF is available here.
PDF is available here.
Journal of Virology (84)1 2010
Abstract
We found diminished association of the polyadenylate-binding protein (PABP) with the cap-binding complex. Although total PABP levels were unchanged, HSV-1 infection prompted accumulation of cytoplasmic PABPC1, but not its physiologic binding partner PABP-interacting protein 2 (Paip2), in the nucleus...
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PMID: 19864386
PDF is available here.
PDF is available here.
Journal of Virology (84)1 2010
Abstract
We generated a panel of HSV-1 promoter-luciferase constructs and measured their response to heat shock. Of the promoters tested, those of ICP0 and ICP22 were the most strongly upregulated after heat shock. Microarray analysis of lytically infected cells supported the upregulation of ICP0 and ICP22 p...
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PMID: 19828605
PDF is available here.
PDF is available here.
Abstract
We recently identified a LITAF-STAT6(B) complex regulated by p38 MAPK in response to LPS stimulation. However, the LPS-induced cascade in the p38/LITAF/TNF signaling pathway remains unclear. Here, we identified PTP4A3, a protein tyrosine phosphotase, as a novel negative regulator of LPS-induced LITA...
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PMID: 20375622
PDF is available here.
PDF is available here.
Abstract
Compelling evidence is accumulating indicating a pathophysiological role of the serum-and-glucocorticoid-inducible-kinase-1 (SGK1) in the development and complications of diabetes. SGK1 is ubiquitously expressed with exquisitely high transcriptional volatility. Stimulators of SGK1 expression include...
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PMID: 19764891
PDF is available here.
PDF is available here.
Oncology Research (17)11 2009
Abstract
Metastasis is a complex, multistep process by which a cancer cell leaves the primary tumor, travels to a distant site via the circulatory system, and establishes a secondary cancer. A deeper understanding of the molecular events underlying metastasis will provide information that will be useful for...
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PMID: 19806791
PDF is available here.
PDF is available here.
Cancer Research (69)15 2009
Abstract
We recently found that the TSC1-TSC2 complex promotes the activity of mTOR complex 2 (mTORC2), an upstream activator of Akt, and this occurs independent of its inhibitory effects on mTORC1. Loss of mTORC2 activity in cells lacking the TSC1-TSC2 complex, coupled with mTORC1-mediated feedback mechanis...
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PMID: 19602587
PDF is available here.
PDF is available here.
Journal of Cell Science (122)Pt 14 2009
Abstract
Abstract
We initially used the E3 ubiquitin ligase, herpes simplex virus type 1 (HSV-1) infected cell protein 0 (ICP0) and one of its targets, promyelocytic leukemia (PML) protein, to determine the feasibility of our approach. Cells expressing a PML-GFP fusion protein were selected by cell sorting and infect...
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PMID: 19504579
PDF is available here.
PDF is available here.
Abstract
We show an age-dependent decline in cortical HIF-1alpha accumulation and activation of HIF target genes in response to hypoxia. This inducible response is significantly attenuated in the cerebral cortex of 18-mo-old Fischer 344 rat yet virtually absent in the cerebral cortex of 24-mo-old Fischer 344...
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PMID: 19420289
PDF is available here.
PDF is available here.
Abstract
Recombinant human HPO and partial hepatectomy rapidly induce the expression of the PC3/BTG2 gene. PC3/BTG2 mRNA is highly expressed in HCC cells and its expression is related to the degree of cell differentiation. The abnormal expression of PC3/BTG2 is closely related to the genesis and development...
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PMID: 19502170
PDF is available here.
PDF is available here.
Gene Therapy (16)6 2009
Abstract
The last two years have seen significant advances in our understanding of the cellular innate responses elicited or activated by the entry of amplicon particles, which may, in part, explain the transient nature of transgene expression often observed in cells infected with helper-free amplicon stocks...
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PMID: 19369969
PDF is available here.
PDF is available here.
Experimental Cell Research (315)9 2009
Abstract
I internalisation and endolysosomal degradation. K5 targets multiple immunoreceptors, including MHC Class I, CD86, intracellular adhesion molecule (ICAM) 1 and MHC Class I-related chain (MIC)-A/B, thereby preventing detection from cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. The cel...
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PMID: 19013150
PDF is available here.
PDF is available here.
Journal of Neuroscience (29)18 2009
Abstract
We show that estradiol regulates auditory processing of acoustic signals in the vertebrate brain, more specifically in the caudomedial nidopallium (NCM), the songbird analog of the mammalian auditory association cortex. Multielectrode recordings coupled with local pharmacological manipulations in aw...
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PMID: 19420261
PDF is available here.
PDF is available here.
Bing du xue bao = Chinese journal of virology / [bian ji, Bing du xue bao bian ji wei yuan hui] (25)3 2009
Abstract
We successfully established a HCMV IE2 protein expression cell line that was controlled by Tet-On system. The effect of IE2 protein on cell apoptosis and the expression of p53 was detected under different condition of induction. Our results showed that the IE2 protein could inhibit cell apoptosis in...
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PMID: 19634761
PDF is available here.
PDF is available here.
Cellular Signalling (21)5 2009
Abstract
We demonstrated that nuclear DGK-zeta downregulated the expression of cyclin D1 and increased the expression of TIS21/BTG2/PC3, a transcriptional regulator of cyclin D1 with a strong anti-proliferative function. Overexpression of TIS21/BTG2/PC3 blocked the cells in G1 phase of the cell cycle and dec...
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PMID: 19709620
PDF is available here.
PDF is available here.
Clinical Cancer Research (15)9 2009
Abstract
We therefore evaluated anti-apoptotic gene expression in tumor samples from patients randomized to dexamethasone or normal saline....
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PMID: 19383827
PDF is available here.
PDF is available here.
Cellular Microbiology (11)4 2009
Abstract
Infection of human hepatitis B virus (HBV) causes acute hepatitis. Its persistent infection leads to a high risk of developing chronic hepatitis, cirrhosis and hepatocellular carcinoma. The levels of HBV 3.5 kb and 2.4/2.1 kb RNAs transcribed from a replicating HBV expression plasmid in human hepato...
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PMID: 19141126
PDF is available here.
PDF is available here.
Abstract
IEX-1 (Immediate Early response gene X-1) is a stress-inducible gene. It suppresses production of reactive oxygen species (ROS) and protects cells from apoptosis induced by a wide range of stimuli, but the underlying mechanism is not known. This study reveals that IEX-1 targets the mitochondrial F1F...
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PMID: 19096392
PDF is available here.
PDF is available here.
Abstract
We have established a cytotoxic sensor cell line by transfecting HepG2 cells with a luciferase protein plasmid derived from the heat shock protein 70B' (HSP70B') promoter, which is induced by cytotoxic reagents. HSP70B genes are up-regulated by a wide-range of cytotoxic stimulators, in particular, t...
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PMID: 19031424
PDF is available here.
PDF is available here.
Abstract
We examined the prevalence of CMV in AAA specimens from 22 patients undergoing surgery and, in five cases, characterized the function of smooth muscle cells (SMCs) from the aneurysm in vitro. Twenty-one (95%) of the 22 AAA specimens were CMV positive by a polymerase chain reaction assay, in situ hyb...
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PMID: 19083194
PDF is available here.
PDF is available here.
Abstract
We further characterized the dose- and time-dependency of radiation-induced expression of IER5 at doses from 0.5 to 15 Gy by quantitative real-time PCR analyses in HeLa cells and human lymphoblastoid AHH-1 cells. A radiation-induced increase in the IER5 mRNA level was evident 2 h after irradiation w...
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PMID: 19238419
PDF is available here.
PDF is available here.
Journal of General Virology (90)Pt 4 2009
Abstract
These results suggest that the ability of RTA to mediate transcriptional activation is distinct from its ability to bind to its target elements....
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PMID: 19223488
PDF is available here.
PDF is available here.
PLoS Pathogens (5)3 2009
Abstract
We define efficient reactivation conditions and demonstrate that reactivation substantially changes the protein synthesis profile. New polypeptide synthesis correlates with 4E-BP1 translational repressor inactivation, nuclear PABP accumulation, eIF4F assembly, and phosphorylation of the cap-binding...
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PMID: 19300492
PDF is available here.
PDF is available here.
Journal of Virology (83)4 2009
Abstract
We sought to examine the functions of ICP27 in the absence of viral infection by creating stable HeLa cell lines that inducibly express ICP27. Here, we characterize two such cell lines and show that ICP27 expression is associated with a cellular growth defect. The observed defect is caused at least...
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PMID: 19073744
PDF is available here.
PDF is available here.
Abstract
Epstein-Barr Virus (EBV) replication and transcription activator (Rta/BRLF1) is an immediate-early transcription factor that controls the conversion of the latent viral genome into one undergoing lytic replication. By using a doxycycline-inducible expression system, the present study demonstrates th...
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PMID: 19098430
PDF is available here.
PDF is available here.
Abstract
We utilized RNAi to knockdown HdmX or Hdm2 in MCF7 human breast cancer cells, which harbor wild-type p53 and elevated levels of HdmX and Hdm2 then examined gene expression changes and effects on cell growth.Cell cycle and growth assays confirmed that the loss of either HdmX or Hdm2 led to a signific...
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PMID: 19946469
PDF is available here.
PDF is available here.