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Viral Structural Proteins (4):
Recent article
Viral Structural Proteins
Abstract
These results suggest that the rotavirus VP6, NSP1 and NSP5 genes of Wa-like rotaviruses are more prone to temporal mutations. Both structural and nonstructural genes of the Western Indian rotavirus strains shared nucleotide and amino acid substitutions with the Bangladeshi strain, Dhaka16-03 (G1P[8...
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PMID: 21256248
PDF is available here.
PDF is available here.
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Abstract
Infections caused by Echovirus 5 (E5), an enterovirus of the Picornaviridae family, have been associated with fever, rashes and sporadic cases of aseptic meningitis. To elucidate the receptor usage of this virus, the significance of a previously proposed integrin binding arginine-glycine-aspartic ac...
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PMID: 20466025
PDF is available here.
PDF is available here.
Abstract
We show that VP22 physically interacted with infected cell polypeptide 0 (ICP0) and colocalized in the nucleus, indicating that VP22 could be functionally involved in the modulation of viral transcription through interaction with ICP0. In the HSV-1 infection system and chloramphenicol acetyltransfer...
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PMID: 20457214
PDF is available here.
PDF is available here.
Journal of Virology (84)16 2010
Abstract
We performed random mutagenesis of PrV pUL36 by transposon-mediated insertion of a 15-bp linker. By this approach, 26 pUL36 insertion mutants were selected and tested in transient transfection assays for their ability to complement one-step growth and/or viral spread of a PrV UL36 null mutant. Ten i...
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PMID: 20534865
PDF is available here.
PDF is available here.
Journal of Virology (84)13 2010
Abstract
We previously reported that the chimeric CE(NiP) strain, which has the phosphoprotein (P protein) gene from the Nishigahara strain in the genetic background of the Ni-CE strain, causes lethal infection in adult mice, indicating that the P gene is responsible for the different pathogenicities of the...
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PMID: 20427527
PDF is available here.
PDF is available here.
Avian Diseases (54)2 2010
Abstract
To determine the expression and distribution of tegument proteins encoded by duck enteritis virus (DEV) UL51 gene in tissues of experimentally infected ducks, for the first time, an immunoperoxidase staining method to detect UL51 protein (UL51p) in paraffin-embedded tissues is reported. A rabbit ant...
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PMID: 20608544
PDF is available here.
PDF is available here.
Archives of Virology (155)6 2010
Abstract
We reached the tentative conclusion that virus-specific dsRNA can inhibit PmDNV replication, in which the dsRNA-ns1 was more effective than the dsRNA-vp....
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PMID: 20336333
PDF is available here.
PDF is available here.
Abstract
Caspases (Casp) activity has been associated with the intracellular proteolytic processing of the structural protein to yield the mature capsid formed by VP70 and with the cell release of human astrovirus (HAstV). This work describes the role of individual Casp on these events. The activity of initi...
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PMID: 20347468
PDF is available here.
PDF is available here.
Abstract
We measured titers of neutralizing (NT) antibody against the Toyama, 2002-240-SF, and prototype Del Carmen E13 strains among inhabitants of Toyama before and after 2002. The rate of positivity for NT antibodies against both 2002-240-SF and Del Carmen in 2003 made a remarkable upturn in children 0 to...
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PMID: 20335430
PDF is available here.
PDF is available here.
Abstract
Our study indicates that recombination events play major roles in enterovirus evolution and that the circulation of multirecombinant strains with unknown properties could be potentially dangerous for public health....
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PMID: 20129960
PDF is available here.
PDF is available here.
Bing du xue bao = Chinese journal of virology / [bian ji, Bing du xue bao bian ji wei yuan hui] (26)3 2010
Abstract
We first synthesized the codon-optimized hemagglutinin gene (HAop) and neuraminidase gene (NAop), both of which were derived from a H5N1 virus (Anhui strain), and constructed successfully the DNA vaccines containing a single cistronic construct (HAwt, HAop, or NAop) or a bicistronic construct (HAop/...
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PMID: 20572336
PDF is available here.
PDF is available here.
Abstract
The routine technique for detecting antibodies specific to infectious bursal disease virus is a serological evaluation by enzyme linked immunosorbent assay (ELISA) with preparations of whole virions as antigens. To avoid the use of complete virus in the standard technique, in-house VP2 and VP3 based...
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PMID: 20152862
PDF is available here.
PDF is available here.
Abstract
RNA interference (RNAi) is a potent mechanism against a variety of viral infections. Infectious bursal disease virus (IBDV) causes an important disease economically in chickens, which is difficult to control. As part of the development of viral vector-mediated RNAi strategy against the disease, five...
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PMID: 19189848
PDF is available here.
PDF is available here.
Abstract
Toggenburg Orbivirus (TOV) has been detected recently in healthy goats in Switzerland. The virus is related closely to bluetongue virus (BTV) and is considered tentatively as a 25th serotype of BTV. Upon detection of additional TOV-positive goats in Switzerland, Germany, and Italy, these TOV isolate...
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PMID: 20219538
PDF is available here.
PDF is available here.
Abstract
These results indicate that sph locus of AMEV can be used successfully to express exogenous genes.
Crown Copyright (c) 2009. Published by Elsevier B.V. All rights reserved....
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PMID: 19477199
PDF is available here.
PDF is available here.
Abstract
We show that vaccinia virus spreads across one cell every 75 minutes, fourfold faster than its replication cycle would permit. To explain this phenomenon, we found that newly infected cells express two surface proteins that mark cells as infected and, via exploitation of cellular machinery, induce t...
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PMID: 20093437
PDF is available here.
PDF is available here.
Abstract
Abstract
These results suggest that UL11 maintains some function(s) when not membrane-bound, and the sequence context of the acylations is important for UL11 function.
Copyright 2009 Elsevier Inc. All rights reserved....
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PMID: 19944438
PDF is available here.
PDF is available here.
Abstract
We show that BDV N assembles into ring- and string-like structures in the presence of 5' genomic BDV RNA. RNA induced polymerization is partly RNA-specific since polymerization is inefficient in the presence of 3' genomic BDV RNA or E. coli RNA. Mutagenesis of basic residues located in the cleft mad...
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PMID: 19945724
PDF is available here.
PDF is available here.
Virus Research (147)2 2010
Abstract
We performed phylogenetic and cluster analysis of human rhinovirus species A (HRV-A) isolated from 76 children with acute respiratory infection in Yamagata prefecture, Japan during the period 2003-2007. Phylogenetic trees based on the nucleotide and amino acid sequences of the VP4/VP2 coding region...
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PMID: 19945490
PDF is available here.
PDF is available here.
Abstract
Two greatly related Lactobacillus plantarum bacteriophages (named FAGK1 and FAGK2) were isolated from Kefir grains of different origins. Both phages belonged to the Siphoviridae family (morphotype B1) and showed similar dimensions for head and tail sizes. The host range of the two phages, using 36 s...
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PMID: 19785909
PDF is available here.
PDF is available here.
Journal of Virology (84)4 2010
Abstract
We analyzed the 2.9-A structure of the VSV N protein bound to RNA (T. J. Green, X. Zhang, G. W. Wertz, and M. Luo, Science 313:357-360, 2006) and identified amino acid residues with the potential to interact with RNA via hydrogen bonds. The contributions of these interactions to N protein function w...
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PMID: 20007268
PDF is available here.
PDF is available here.
Journal of Virology (84)4 2010
Abstract
Although few simian rotaviruses (RVs) have been isolated, such strains have been important for basic research and vaccine development. To explore the origins of simian RVs, the complete genome sequences of strains PTRV (G8P[1]), RRV (G3P[3]), and TUCH (G3P[24]) were determined. These data allowed th...
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PMID: 19939934
PDF is available here.
PDF is available here.
Journal of Virology (84)4 2010
Abstract
We report here the 3.4-A resolution crystal structure of a subviral particle (SVP) of IPNV, containing 20 VP2 trimers organized with icosahedral symmetry. We show that, as expected, the SVPs have a very similar organization to the IBDV counterparts, with VP2 exhibiting the same overall 3D fold. Howe...
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PMID: 20007275
PDF is available here.
PDF is available here.
Journal of Virology (84)4 2010
Abstract
We characterized the effects of the active leflunomide metabolite A771726 (LEF-A) on the polyomavirus BK (BKV) life cycle in human renal tubular epithelial cells. LEF-A at 10 microg/ml reduced the extracellular BKV load by 90% (IC(90)) but with significant host cytostatic effects. BKV genome replica...
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PMID: 19955306
PDF is available here.
PDF is available here.
EMBO Journal (29)2 2010
Abstract
We have determined the structure of polyhedra of the baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV), revealing a highly symmetrical covalently cross-braced robust lattice, the subunits of which possess a flexible adaptor enabling this supra-molecular assembly to specifical...
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PMID: 19959989
PDF is available here.
PDF is available here.
Acta Virologica (54)2 2010
Abstract
Phylogenetic analysis of nucleotide sequences or deduced amino acid sequences of phosphoprotein (P protein), matrix (M) protein, and glycoprotein (G protein) genes of 18 Chinese isolates of Rabies virus (RABV) from 2003-2007 showed that these isolates formed a separate monophyletic lineage consistin...
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PMID: 20545436
PDF is available here.
PDF is available here.
Abstract
Noroviruses (NoVs) resembling human NoV genotype GIV (Alphatron-like) have recently been detected in carnivores. By using an enzyme-linked immunosorbent assay based on baculovirus-expressed capsid protein VP1 of lion strain GGIV.2/Pistoia/387/06/ITA, NoV-specific antibodies were detected in cats (16...
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PMID: 19923574
PDF is available here.
PDF is available here.
Abstract
Immunofluorescence assays (IFAs) for detection of human bocavirus (HBoV) proteins (VP1, VP2, NP-1, and NS1) were developed. The VP1 IFA was the most sensitive for detection of IgG antibody and suitable for screening. IgG antibodies in convalescent-phase sera from HBoV-positive patients were detected...
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PMID: 19955324
PDF is available here.
PDF is available here.
Abstract
A new variation of the vp2 gene exists in the MEV-DL strain, which may lead to structural changes of the VP2 protein. Phylogenetic analysis showed that MEV-DL may originate from the ZYL-1 strain in DaLian....
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PMID: 20540765
PDF is available here.
PDF is available here.
Voprosy Virusologii (55)2 2010
Abstract
The paper analyzes a 994 nucleotide fragment of the NS1/VP1u region junction of 84 parvovirus B19 samples obtained from the sera of erythema infectiosum patients in Belarus in 2006. All the strains belong to genotype 1 as defined by Servant et al. (2002) and form two major clusters within this genot...
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PMID: 20455468
PDF is available here.
PDF is available here.
Voprosy Virusologii (55)1 2010
Abstract
The review describes the phenotypic properties, structure, and expression pattern of West Nile virus genome (Flaviviridae, Flavivirus, Japanese encephalitis antigenic complex), as well as the clinical picture and pathogenesis of its etiologically related disease West Nile fever. It also analyzes the...
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PMID: 20364664
PDF is available here.
PDF is available here.
Abstract
Baculovirus expression vectors are extensively used for the delivering foreign genes and expression of recombinant proteins in insect and mammalian cells. Modified baculoviruses containing mammalian promoter elements (BacMam viruses) for an efficient transient and stable transduction of diverse mamm...
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PMID: 20608180
PDF is available here.
PDF is available here.
Abstract
A PCR-test system for detection of primate Betaherpesvirinae viruses was developed. Using alignment of complete genome of human, chimpanzee, and macaque rhesus cytomegalovirus conserve regions of viral genes were found. The oligonucleotide primers for consensus conserve regions of CMV UL56 gene were...
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PMID: 20882773
PDF is available here.
PDF is available here.
Ukrainskij biokhimicheskij zhurnal/Ukrayins'kij biokhimichnij zhurnal/Ukrainian biochemical journal (82)6 2010
Abstract
It was shown that two small RNAs about 65 and 55 nucleotides long included in NPV B. mori polyhedra form with polypeptides p29 and p14 specific RNP-complexes with molecular weights of 50 and 31 kDa, respectively. Both complexes form high-molecular weight complex with polyhedrin. Origin and nature of...
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PMID: 21805866
PDF is available here.
PDF is available here.
Journal of Virology (84)1 2010
Abstract
We analyzed, by means of an IPTG (isopropyl-beta-d-thiogalactopyranoside)-inducible virus, the role of polyprotein pp62 in virus assembly. Polyprotein pp62 is indispensable for viral replication. The repression of polyprotein pp62 expression does not alter late gene expression or the proteolytic pro...
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PMID: 19846532
PDF is available here.
PDF is available here.
Journal of Virology (84)2 2010
Abstract
We investigated the functions of the largest of the accessory proteins, the ORF 3a protein, using a 3a-deficient strain of SARS-CoV. Cell death of Vero cells after infection with SARS-CoV was reduced upon deletion of ORF 3a. Electron microscopy of infected cells revealed a role for ORF 3a in SARS-Co...
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PMID: 19889773
PDF is available here.
PDF is available here.
Journal of Virology (84)2 2010
Abstract
Our results emphasize the evolutionary and genetic divergences between members of the NA and SA EEEV lineages, consistent with major differences in pathogenicity and ecology, and propose that NA and SA EEEV be reclassified as distinct species in the EEE complex....
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PMID: 19889755
PDF is available here.
PDF is available here.
Abstract
The porcine adenovirus NADC-1 isolate, a strain of porcine adenovirus type 4, has a fibre with an atypical architecture. In addition to a classical virus attachment region, shaft and head domains, it contains an additional galectin like domain C-terminal to the head domain and connected to the head...
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PMID: 19923738
PDF is available here.
PDF is available here.
Emerging Infectious Diseases (15)11 2009
Abstract
A fatal case of enterovirus 71 infection with pulmonary edema and rhombencephalitis occurred in Brest, France, in April 2007. The virus was identified as subgenogroup C2. This highly neurotropic enterovirus merits specific surveillance outside the Asia-Pacific region.
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PMID: 19891879
PDF is available here.
PDF is available here.
Abstract
Immunohistochemical (IHC) and fluorescent antibody (FA) techniques were optimized for the detection of Foot-and-mouth disease virus (FMDV) structural and nonstructural proteins in frozen and paraformaldehyde-fixed, paraffin-embedded (PFPE) tissues of bovine and porcine origin. Immunohistochemical lo...
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PMID: 19901278
PDF is available here.
PDF is available here.
Abstract
I based one-step real-time reverse transcriptase polymerase chain reaction was developed for the detection and differentiation of very virulent (vv) and classical strains of infectious bursal disease virus (IBDV). The assay showed high PCR efficiency >93% and high reproducibility with coefficient of...
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PMID: 19591873
PDF is available here.
PDF is available here.
Abstract
All the detected HAV strains were identified as sub-genotype I A, the homology of nucleotide sequence in the VP1-2A imation region ranged from 95%-100%, the amino acid sequences of HAV strains almost had no difference. CONCLUSION: There are different HAV strains existing in Hebei Shijiazhuang of Chi...
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PMID: 20387485
PDF is available here.
PDF is available here.
Abstract
The nucleotide sequence differences in the VP1-2A region among Xinjiang Hetian HAV strains ranged from 0%-3.9%, all belonged to sub-genotype 1A. Genetically similar strains were identified among Xinjiang Hetian 2006 and Xinjiang Yili 2005 of China isolates. Only 0-2 amino acid differences were found...
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PMID: 20387486
PDF is available here.
PDF is available here.
Abstract
Sodium Dodecyl Sulphate Poly-Acrylamide Gel Electrophoresis (SDS-PAGE) and Western blot results showed that the VP2 gene was successfully expressed in chicken primary myoblast cells. The product was a 48kDa protein and could be recognized by anti-IBDV serum. CONCLUSION: The recombinant baculovirus c...
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PMID: 20030068
PDF is available here.
PDF is available here.
Avian Diseases (53)3 2009
Abstract
This retrospective study concerned 41 infectious bursal disease virus (IBDV) isolates obtained from Brazilian broiler and layers flocks by reverse transcription-polymerase chain reaction. Twenty-five of them were identified as very virulent (vv) by restriction enzyme analysis and by further nucleoti...
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PMID: 19848087
PDF is available here.
PDF is available here.
Abstract
We characterized the genetic diversity of the complete VP1 region of coxsackievirus A16 (CA16) and enterovirus 71 (EV71) isolated from patients with hand, foot, and mouth disease in Toyama from 1981 to 2007 to evaluate the relationship between epidemics and genetic changes. The predominant genogroup...
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PMID: 19628900
PDF is available here.
PDF is available here.
Bing du xue bao = Chinese journal of virology / [bian ji, Bing du xue bao bian ji wei yuan hui] (25)4 2009
Abstract
Based on the infectious clone of JEV vaccine strain SA14-14-2, prM-E genes and C-prM-E genes were cloned into pCDNA3.1 vector. The recombinant plasmid pCJE-ME was transfected into BHK-21 cells, the expressed proteins were toxic to the cell growth and accelerated the cell death. But when transfected...
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PMID: 19769161
PDF is available here.
PDF is available here.
Abstract
We describe the secretory expression and immunogenicity of the recombinant HAV (hepatitis A virus) structural polypeptide VP1 from stably transformed Drosophila melanogaster S2 (Schneider 2) cells. Southern-blot analysis indicated that transformed S2 cells contained multiple copies of the HAV VP1 ge...
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PMID: 18652573
PDF is available here.
PDF is available here.
Virus/Uirusu (59)1 2009
Abstract
We summarize the current knowledge of HIV accessory proteins and their cellular targets, and discuss the functional roles of these biologically unique and important viral proteins for virus replication in vitro and in vivo....
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PMID: 19927991
PDF is available here.
PDF is available here.
Abstract
The four gene fragments of the genome were amplified and cloned into the expression vector. The recombinant genome cDNA plasmid pCTN-GFP was constructed and subjected to restriction endonuclease digestions. After sequenced to assure no absence and mutations compared with their parental viruses, it w...
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PMID: 19799148
PDF is available here.
PDF is available here.
Abstract
To investigate whether a 12 nucleotide DNA-based miniEGSs can silence the expression of human cytomegalovirus (HCMV) UL49 gene efficiently, A HeLa cell line stably expressing UL49 gene was constructed and the putative miniEGSs (UL49-miniEGSs) were assayed in the stable cell line. Qua...
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PMID: 19430703
PDF is available here.
PDF is available here.
Abstract
To investigate whether a 12 nucleotide DNA-based miniEGSs can silence the expression of human cytomegalovirus (HCMV) UL49 gene efficiently, A HeLa cell line stably expressing UL49 gene was constructed and the putative miniEGSs (UL49-miniEGSs) were assayed in the stable cell line. Qua...
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PMID: 19430703
PDF is available here.
PDF is available here.
Abstract
The genome of DHV-N G consists of 7774 nucleotides excluding a poly (A) tail of 12 nucleotides. It contains a single large open reading frame encoding a polypeptide of 2251 amino acid residues. The length of 3' UTR of DHV-N G is 366 nucleotides, which is 52 nucleotides longer than that of DHV-1 C80...
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PMID: 19623953
PDF is available here.
PDF is available here.
Viral Immunology (22)1 2009
Abstract
Journal of Virology (83)4 2009
Abstract
We have used internal ribosome entry sites (IRES) from picornaviruses to control the expression level of the phosphoprotein P of the neurotropic rabies virus (RV; Rhabdoviridae), which is critically required for both viral replication and escape from the host interferon response. In a dual luciferas...
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PMID: 19073737
PDF is available here.
PDF is available here.
Journal of Virology (83)4 2009
Abstract
We generated a recombinant vaccinia virus that expresses an F12, which lacks residues 351 to 458. Characterization of this recombinant revealed a small-plaque phenotype and a subsequent defect in virus release similar to a recombinant virus that had F12L deleted. In addition, F12 lacking residues 35...
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PMID: 19052096
PDF is available here.
PDF is available here.
Journal of Virology (83)4 2009
Abstract
We investigated the roles of viral and cellular proteins in the process of capsid-nucleus attachment. Vero cells were preloaded with antibodies specific for proteins of interest and infected with HSV-1 containing a green fluorescent protein-labeled capsid, and capsids bound to the nuclear surface we...
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PMID: 19073727
PDF is available here.
PDF is available here.
Abstract
Following administration of this vaccine to Balb/c mice, an approximately ten-fold increase in antibody response was elicited and increased protective efficacy compared to an ad-based vaccine containing non-optimised genes was observed after challenge. CONCLUSION: This study, in which the utility of...
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PMID: 19646224
PDF is available here.
PDF is available here.
Abstract
We investigated here the specific effect of the neurotrophins on P protein, evaluating its synthesis and subcellular distribution in adult mouse dorsal root ganglia neuron cultures infected and treated with NGF or NT-3. The results showed that NGF, but not NT-3, caused an increase in the quantity of...
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PMID: 19283595
PDF is available here.
PDF is available here.