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Hemolymph (0):
Recent article
Hemolymph
Journal of Insect Physiology (56)11 2010
Abstract
We demonstrated that sex pheromone production in mated female Heliothis virescens moths is dependent upon hemolymph trehalose concentration (HTC), which is influenced by activities such as the feeding of adults on sucrose. In this paper we demonstrate, for the first time, that this effect also occur...
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PMID: 20540946
PDF is available here.
PDF is available here.
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Abstract
We previously demonstrated that jack bean urease (JBU) has antidiuretic effects on Rhodnius prolixus Malpighian tubules, decreasing the serotonin-stimulated secretion of fluid. Now, we evaluate the toxicity of the intact JBU and its effect on R. prolixus anterior midgut, to further elucidate the mec...
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PMID: 20223243
PDF is available here.
PDF is available here.
Abstract
We purified the sialic acid-specific lectin(s) from the pupal hemolymph using formaldehyde-fixed HRBC and determined the sequence of the amino-terminal 19 amino acid residues. A polyclonal antibody produced against this N-terminal peptide clearly inhibited the hemolytic activity of the hemolymph in...
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PMID: 20193690
PDF is available here.
PDF is available here.
Abstract
We examined the impact of melanized encapsulation, a component of innate immunity that integrates both cellular and humoral immune responses, on the success of the baculovirus Lymantria dispar multiple nucleocapsid nucleopolyhedrovirus (LdMNPV) in its host L. dispar. L. dispar exhibits midgut-based...
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PMID: 20350551
PDF is available here.
PDF is available here.
Abstract
We have a detailed understanding of invertebrate immune responses to bacteria and fungal pathogens, but we know less about how insects respond to virus challenge. Phenoloxidase (PO) functions as an important immune response against many parasites and pathogens and is routinely used as a measure of i...
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PMID: 20380834
PDF is available here.
PDF is available here.
Abstract
We physiologically characterized CSH and pharmacological action of tungstate, using a nymphalid butterfly Junonia orithya. We first showed that the precise patterns of modification were dependent on the time-point of the cold-shock treatment after pupation, and confirmed that the modification proper...
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PMID: 20206631
PDF is available here.
PDF is available here.
Abstract
We provide genetic evidence that Drosophila Ide (dIde) antagonizes the insulin signaling pathway and human Abeta-induced neurotoxicity in Drosophila. In this study, we also generated a dIde knockout mutant (dIde(KO)) by gene targeting, and found that loss of IDE increases the content of the major in...
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PMID: 20493190
PDF is available here.
PDF is available here.
Abstract
We then determined the full-length cDNA sequence of Sp-SPH, which contained 1298bp with an open reading frame of 1107bp encoding 369 amino acid residues. Multiple alignment analysis showed that the deduced amino acid sequences of Sp-SPH shared overall identity (83.8%) with those of SPH-containing pr...
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PMID: 20153768
PDF is available here.
PDF is available here.
Abstract
This paper evaluates the alterations in the glycogen content of tissues (digestive gland and cephalopedal mass) and glucose in the haemolymph of Biomphalaria glabrata BH strain infected with Schistosoma mansoni BH strain and exposed to the latex of Euphorbia splendens var. hislopii. A reduction in t...
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PMID: 20721497
PDF is available here.
PDF is available here.
Abstract
We detected two distinct spots in hemolymph from septic injured larvae (HDs1 and HDs2), which are separated by 2DE gel electrophoresis. Both spots were subjected to in-gel tryptic digestion and MALDI-TOF/TOF analysis, which revealed the sequence VFGTLGSDDSGLFGK present in both HDs1 and HDs2. This se...
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PMID: 20490430
PDF is available here.
PDF is available here.
Journal of Biochemistry (147)5 2010
Abstract
We know that LPS-induced haemocyte exocytosis leads not only to coagulation but also activates a sophisticated immune response network that coordinately induces pathogen recognition, elimination and wound healing....
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PMID: 20406733
PDF is available here.
PDF is available here.
Abstract
Plant extracts are currently studied more and more because of the possibility of their usage in plant protection. Many of the natural plant compounds which are used in the control of pests are known to affect the digestion and immune functions of insects. In this study, effects of Artemisia annua ex...
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PMID: 19519976
PDF is available here.
PDF is available here.
Abstract
The hemagglutination activities of midgut (MG) fractions of red palm weevil, Rhynchophorus ferrugineus against mammalian erythrocytes (RBC) from man (ABO), rabbit, horse and sheep were studied. The highest titers were seen with rabbit RBC (p < 0.05) followed by human group B, human group O, horse, h...
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PMID: 20464944
PDF is available here.
PDF is available here.
Abstract
We have observed, however, that the resting membrane potential of muscle cells in the larval bodywall of Drosophila melanogaster varies widely as the external calcium concentration is modified. The RMP depolarized as much as 21.8 mV/mM calcium at low concentrations, and on average, about 10 mV/mM ac...
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PMID: 19913024
PDF is available here.
PDF is available here.
Abstract
The influence of different photophases (0, 6, 12, 18 and 24 hours) on the triglycerides and total cholesterol contents in the hemolymph of A. fulica was evaluated, since there is no information in the literature about the influence of this factor on lipids metabolism in mollusks. After 2 and 4 weeks...
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PMID: 20231969
PDF is available here.
PDF is available here.
Abstract
For the first time, a morphological study of haemocytes from the crab Carcinus aestuarii was carried out by means of light microscopy and differing cytochemical assays. Analysis of haemocyte size frequency distribution (performed by means of a Coulter Counter) revealed the presence of two distinct h...
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PMID: 20353916
PDF is available here.
PDF is available here.
Tsitologiia (52)6 2010
Abstract
I appear after crop emptying. Immunization has a positive effect on their differentiation rate and, correspondently, the rapid progress in the defense response. The proportion of competent plasmatocytes in immunized larvae increases. The stable hyaline cells formation also depends on immunization, a...
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PMID: 20737902
PDF is available here.
PDF is available here.
Tsitologiia (52)12 2010
Abstract
Biological activity of alloferon (AF), peptide, consisting of 13 a. a. isolated from hemolymph of experimentally infected blow fly Calliphora vicina was studied. AF in concentrations form 1 x 10(-5) to 250 microg/ml was added into the culture medium of the target cell lines K562, J-9...
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PMID: 21427979
PDF is available here.
PDF is available here.
Abstract
We used the greater wax moth Galleria mellonella, which can be reared at 37 degrees C, as a model host for examining the virulence potential of Listeria spp. Here we report that Galleria is an excellent surrogate model of listerial septic infection, capable of clearly distinguishing between pathogen...
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PMID: 19897755
PDF is available here.
PDF is available here.
Abstract
Activities of enzymatic antioxidants (superoxide dismutase, glutathione-S-transferase, and catalase) have been determined in hemocytes and generation of reactive oxygen species (ROS) has been studied in lymph of larvae of the wax moth Galleria mellonella at development of the process of encapsulatio...
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PMID: 20297667
PDF is available here.
PDF is available here.
Abstract
The work deals with study of basic characteristics of cardiac activity (HR and stress-index) and hemolymph protein parameters in the crayfish Pontastacus leptodactylus. The main criteria of the crayfish selection are developed for formation of the animal reference groups available for subsequent tox...
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PMID: 20583580
PDF is available here.
PDF is available here.
Abstract
The application of hematological analysis techniques to detecting the physiological status of the economically valued decapods during their culturing, and in monitoring of the condition of their natural populations, is restrained by the incomplete knowledge of these invertebrates circulatory system...
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PMID: 20469604
PDF is available here.
PDF is available here.
Abstract
These results are consistent with Na(+)-dependent absorption of amino acids and dicarboxylic acids. Effects of serotonin withdrawal indicated that nutrient absorption is stimulated by this hormone. Strong luminal alkalinization was observed with mosquito saline containing serotonin on the hemolymph-...
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PMID: 19637352
PDF is available here.
PDF is available here.
Environmental Entomology (38)4 2009
Abstract
The Asiatic rice borer Chilo suppressalis (Walker) occurs mainly on rice Oryza sativa L. and water-oat Zizania latifolia (Turcz). Certain ecological and physiological differentiations between rice and water-oat populations have been shown. To determine whether there is host-associated differentiatio...
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PMID: 19689898
PDF is available here.
PDF is available here.
Abstract
We report on the transmission of Hematodinium sp. into blue crabs that were fed pieces of infected tissues and examined for evidence of infection at time periods from 1 to 48 h and for the general state of their health after 4 d. During the first 16 h after feeding, Hematodinium sp. was found in the...
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PMID: 19750807
PDF is available here.
PDF is available here.
Parasitology (92 ( Pt 3))03 2009
Abstract
The tegumental surface of Schistosoma mansoni sporocysts is the site of both nutritive and immunological interactions with haemolymph cells and plasma of Biomphalaria glabrata, the schistosome intermediate host. Within minutes of being placed in host plasma, sporocysts acquire plasma antigens, and w...
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PMID: 2426650
PDF is available here.
PDF is available here.
Abstract
C-type lectins are Ca(2+) dependent carbohydrate-recognition proteins that play crucial roles in the invertebrate innate immunity, such as nonself recognition, activation of proPO system, antibacterial activity, promotion of phagocytosis and nodule formation. In this study, a novel C-type lectin of...
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PMID: 18622759
PDF is available here.
PDF is available here.
Parasitology (92 ( Pt 2))02 2009
Abstract
This study describes experiments which demonstrate the presence of a parasite-induced or derived factor in the haemocoel of Onchocerca lienalis-infected simuliids. Haemolymph, when transferred from previously infected donor flies to previously untreated recipient flies, using fine glass needles, con...
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PMID: 3714299
PDF is available here.
PDF is available here.
Journal of Fish Diseases (32)4 2009
Abstract
Marteilia sydneyi is the causative agent of QX disease in Sydney rock oyster, Saccostrea glomerata. It is responsible for disease outbreaks among oysters that occur during summer and can result in up to 95% mortality. QX disease has significantly decreased S. glomerata production in some areas of Au...
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PMID: 19335613
PDF is available here.
PDF is available here.
Abstract
Recent insights into the allosteric control of oxygen binding in the extracellular hemoglobin (Hb) of the tadpole shrimp Triops cancriformis raised the question about the physico-chemical properties of the protein's native environment. This study determined the cationic composition and acid-base sta...
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PMID: 19066911
PDF is available here.
PDF is available here.
Abstract
A study about the relationship between the physical-chemical parameters and the calcium carbonate balance between the haemolymph fluid and mantle calcareous structures was carried out in Anodonta cygnea. An intense peak of HCO(3) (-) and a highest pH in December-January months may be understood as a...
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PMID: 18985356
PDF is available here.
PDF is available here.
Aquatic Toxicology (91)4 2009
Abstract
We characterized ionoregulation and acid-base balance in Pb-exposed L. stagnalis (young adults approximately 1g) to investigate the mechanisms underlying this hypersensitivity. After 21-day exposure to 18.9microgl(-1)Pb, Ca(2+) influx was significantly inhibited (39%) and corresponding net Ca(2+) fl...
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PMID: 19056133
PDF is available here.
PDF is available here.
Abstract
Yellow head virus (YHV) is a highly virulent pathogen of penaeid shrimp. An isolate obtained from Penaeus vannamei during a yellow head disease outbreak in February 2006 in Ratchaburi Province, Thailand was purified following passage in experimentally infected shrimp. SDS-PAGE of pur...
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PMID: 19049843
PDF is available here.
PDF is available here.
High-level expression of orange fluorescent protein in the silkworm larvae by the Bac-to-Bac system.
Abstract
We used infection technique which introduced the baculovirus DNA into silkworms using a cationic lipofectin reagent instead of directly injecting the virus, and demonstrated a high-level expression of the orange fluorescent protein (OFP) gene in the Bombyx mori, silkworm larvae. When recombinant rBa...
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PMID: 18034370
PDF is available here.
PDF is available here.
Abstract
The behaviour of the albino and melanic variants of Biomphalaria glabrata of Belo Horizonte (MG. Brazil) was studied comparatively, in terms of their respective susceptibilities to infection by Schistosoma mansoni of the same origin, through observation of the elimination of cercariae for a three-mo...
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PMID: 19347168
PDF is available here.
PDF is available here.
Abstract
We also noted that the antimicrobial activity level induced by the non-pathogenic bacterium was higher in comparison to that measured in insects infected with the pathogenic strain of P. aeruginosa. The results of our in vitro studies indicated that inducible antimicrobial peptides of G. mellonella...
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PMID: 18996217
PDF is available here.
PDF is available here.
Journal of Parasitology (95)1 2009
Abstract
Leeches exhibit a marked scope of diversity, including different kinds of symbiosis. The aim of the present study was to demonstrate through biochemical and histological analysis that a species of piscicolid leech, Myzobdella platensis, is a true parasite of blue crabs, feeding on their hemolymph an...
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PMID: 18601577
PDF is available here.
PDF is available here.
Abstract
This work deals with the antibacterial activity of larvae of Galleria mellonella. Protein fractions with antibacterial activity have been purified from hemolymph by ion-exchange chromatography and gel-electrophoresis. The mass-spectrometric analysis of fractions showed the presence o...
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PMID: 20469719
PDF is available here.
PDF is available here.
BMC Physiology (9)1 2009
Abstract
With the use of microbubbles as high contrast tracer particles, we directly visualized hemolymph flow in a grasshopper (Schistocerca americana) using synchrotron x-ray phase-contrast imaging. In-vivo intra-heart flow patterns and the relationship between respiratory (tracheae and air sacs) and circu...
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PMID: 19272159
PDF is available here.
PDF is available here.
Abstract
Seven sets of recombinant expression vectors were constructed for the expression of the human anti-bovine serum albumin (BSA) single-chain Fv fragment (scFv) 13CG2 fused to the C terminus of GFP(uv) or bombyxin (bx) signal peptide in the hemolymph and fat body of silkworm larvae and pupae, using cys...
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PMID: 19147113
PDF is available here.
PDF is available here.
Archives of Virology (154)3 2009
Abstract
We tested whether this was the case for pea enation mosaic virus (PEMV) virions in the pea aphid (Acyrthosiphon pisum) using RNA1Delta, a natural deletion mutant lacking the middle portion of the RTD ORF, and CPDeltaRTD, in which the entire RTD ORF was deleted. In infected plants, RNA1Delta virions...
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PMID: 19240978
PDF is available here.
PDF is available here.
Journal of Biotechnology (139)1 2009
Abstract
A Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid expressing heavy and light chains of human 29IJ6 IgG was constructed and used to secrete recombinant antibody into silkworm larval hemolymph. Fifth instar silkworm larvae were reared and injected into the dorsum of the larvae with recombinant cystein...
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PMID: 18984019
PDF is available here.
PDF is available here.
Abstract
Fluorophores from the hemolymph of yellow sac spiders (Cheiracanthium mildei) have been characterized using excitation emission matrix (EEM) fluorescence spectroscopy. This approach provides characterization of fluorophores present in the organism without having to isolate pure samples. Minimal vari...
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PMID: 18819817
PDF is available here.
PDF is available here.
FEMS Microbiology Letters (289)1 2008
Abstract
Vibrio harveyi is a bacterial marine pathogen that can cause fatal disease in a large range of vertebrates and invertebrates, including the commercially important marine gastropod, Haliotis tuberculata. Since 1997, strains of this bacterium have regularly been causing high mortalities in farmed and...
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PMID: 19054091
PDF is available here.
PDF is available here.
Abstract
These results indicate that S. littoralis ACE may influence trypsin biosynthesis in the larval gut by interacting with a trypsin-modulating oostatic factor (TMOF). Injecting third instar larvae with a combination of Aea-TMOF and the ACE inhibitor captopril, down-regulated trypsin biosynthesis in the...
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PMID: 18949805
PDF is available here.
PDF is available here.
Environmental Pollution (156)3 2008
Abstract
We searched for an explanation by analyzing hemolymph U-transport in Corbicula exposed to 0.36 microM dissolved uranium at various O2-levels for 10 days. In hypoxia, one observed an increased U concentration in the arterial hemolymph flowing from gills to tissues but this was not associated with an...
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PMID: 18583003
PDF is available here.
PDF is available here.
Acta Biologica Hungarica (59)4 2008
Abstract
We examined brain-suboesophageal ganglia and corpora cardiaca-corpora allata complexes of the cabbage moth, Mamestra brassicae, in order to obtain clues about possible peptide candidates which may be appropriate for the biological control of this pest. With the aid of bioassays, reversed phase high...
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PMID: 19133496
PDF is available here.
PDF is available here.
Abstract
We used RNA interference to silence the expression of the defensin varisin produced by the hemocytes of the American dog tick, Dermacentor variabilis. Ticks were injected with double stranded varisin RNA prior to being placed on a rabbit. After feeding, the ticks were removed, bled, and the hemolymp...
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PMID: 18506584
PDF is available here.
PDF is available here.
Abstract
The effects of prolonged emersion (24, 48 and 72 h) and subsequent re-immersion on Nephrops norvegicus (L.) held at 5 degrees C were assessed using an index of physical quality criteria and a suite of haemolymph constituent assays. Collectively, these showed classical hypoxia-induced changes over 48...
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PMID: 19149376
PDF is available here.
PDF is available here.
Abstract
We examined the free amino acid composition of hemolymph from representatives of five spider families with an interest in knowing if the amino acid profile in the hemolymph of orb-web-building spiders reflects the high demands for small organic compounds in the sticky droplets of their webs. In near...
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PMID: 18692589
PDF is available here.
PDF is available here.
Abstract
We studied the effects of five biogenic amines - norepinephrine, dopamine, octopamine, serotonin and histamine - on the locomotory activity and mobilization of lipids in the adult females of the firebug, Pyrrhocoris apterus (L.). We tested the hypothesis (1) whether the stimulation of walking activi...
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PMID: 18707014
PDF is available here.
PDF is available here.
Journal of Chemical Ecology (34)11 2008
Abstract
Gas chromatography-mass spectrometry (GC-MS) and GC-electroantennographic detection (EAD) analyses of the sex pheromone extract from a wasp moth, Syntomoides imaon (Lepidoptera: Arctiidae: Syntominae), showed that virgin females produced (Z,Z,Z)-3,6,9-henicosatriene and (Z,Z,Z)-1,3,6,9-henicosatetra...
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PMID: 18839254
PDF is available here.
PDF is available here.
Abstract
The tasar silkworm, Antheraea mylitta Drury, Andhra local ecorace is an exclusive race of Andhra Pradesh. It is on the verge of extinction due to difficulty of acclimatisation at breeding and rearing stages. As an attempt to protect this race, a method of total indoor rearing has been done. In this...
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PMID: 19297987
PDF is available here.
PDF is available here.
Abstract
Ascoviruses are double-stranded DNA viruses which cause fatal disease in lepidopteran host larvae. They induce a unique pathology, causing cleavage of host cells into virion-containing vesicles. With the single exception of Diadromus pulchellus ascovirus, all ascoviruses have been exclusively report...
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PMID: 18725228
PDF is available here.
PDF is available here.
Environmental Microbiology (10)10 2008
Abstract
The concept of polymicrobial disease is well accepted in human and veterinary medicine but has received very little attention in the field of aquaculture. This study was conducted to investigate the synergistic effect of white spot syndrome virus (WSSV) and Vibrio campbellii on development of diseas...
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PMID: 18616551
PDF is available here.
PDF is available here.
Abstract
Prophenoloxidase (PPO) was isolated from the hemolymph of Ostrinia furnacalis larvae and purified to homogeneity. A 369.85-fold purification and 35.34% recovery of activity were achieved by employing ammonium sulfate precipitation, Blue Sepharose CL-6B chromatography and Phenyl Sepharose CL-4B chrom...
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PMID: 18577463
PDF is available here.
PDF is available here.
Abstract
We compared systematically the temporal and dose response relationship and physiological significance among biogenic amines injection, changes of ion concentration, FAA concentrations and composition and protein in context of osmoregulatory ability in marine euryhaline shrimp: Litopenaeus vannamei....
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PMID: 18634897
PDF is available here.
PDF is available here.
Abstract
Eleven Vibrio isolates invading the hemolymph of live and moribund oysters (Crassostrea gigas) collected in the field and from a hatchery in France, were characterized by a polyphasic approach. Phylogenetic analysis of 16S rRNA, gyrB and toxR genes indicated high homogeneity between these strains an...
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PMID: 18718730
PDF is available here.
PDF is available here.
Abstract
Our microarray analysis shows that compared to oocyst sporozoites, salivary gland sporozoites upregulate expression of at least 124 unique genes. Conversely, oocyst sporozoites show upregulation of at least 47 genes (upregulated in oocyst sporozoites [UOS genes]) before they infect the salivary glan...
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PMID: 18710954
PDF is available here.
PDF is available here.
Experimental Parasitology (120)2 2008
Abstract
Live adult and juvenile entomopathogenic Steinernema carpocapsae DD136 (P. Nematoda) were not subjected to adhesion by haemocytes of lepidopteran insect larvae of Galleria mellonella or Malacosoma disstriain vitro or in vivo. In vitro freeze-killed nematodes exhibited haemocyte attachment, the inten...
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PMID: 18656470
PDF is available here.
PDF is available here.