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Fireflies (0):
Recent article
Fireflies
Abstract
We consider substrate-related compounds, intermediates or products of the Luc catalyzed reactions, in addition to anesthetics and, fatty acids. This review summarizes the main inhibitors of Luc and the corresponding inhibition kinetic parameters.
Copyright 2010 Elsevier B.V. All rights reserved....
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PMID: 20655239
PDF is available here.
PDF is available here.
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Abstract
The thermal sensitivity and pH-sensitive spectral properties of firefly luciferase have hampered its application in a variety of fields. It is proposed that the stability of a protein can be increased by introduction of disulfide bridge that decreases the configurational entropy of unfolding. A disu...
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PMID: 20593108
PDF is available here.
PDF is available here.
Abstract
We studied synchrony's behavioral role in the North American firefly, Photinus carolinus. We placed a female in a virtual environment containing artificial males that flashed at varying degrees of synchrony. Females responded to an average of 82% of synchronous flashes compared with as few as 3% of...
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PMID: 20616271
PDF is available here.
PDF is available here.
Abstract
When a firefly is made to inhale ethyl acetate vapour, a constant glow appears after a few minutes from its abdominal lantern. This control experiment has been performed by a few workers to record the emission spectrum of the firefly. However, a time-resolved experiment performed by us on this conti...
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PMID: 20689174
PDF is available here.
PDF is available here.
Abstract
We describe an in vitro translation assay that reconstitutes the translational repression activity of canonical PUF proteins. In this system, recombinant PUF proteins were added to yeast cell lysates to repress reporter mRNAs bearing the 3'UTRs of specific target mRNAs. PUF proteins from Saccharomyc...
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PMID: 20427513
PDF is available here.
PDF is available here.
Abstract
We prove that for pulse-coupled oscillator networks with delayed excitatory coupling, weak asymptotic synchronization cannot occur....
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PMID: 20039465
PDF is available here.
PDF is available here.
Abstract
The crystal structures of the pure, unsubstituted firefly emitter oxyluciferin (OxyLH(2)) and its 5-methyl analogue (MOxyLH(2)) were determined for the first time to reveal that both molecules exist as pure trans-enol forms, enol-OxyLH(2) and enol-MOxyLH(2), assembled as head-to-tail hydrogen-bonded...
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PMID: 19722653
PDF is available here.
PDF is available here.
Abstract
We discuss the use of the luciferin/luciferase-based reaction to measure extracellular ATP concentrations with high sensitivity. Protocols are adapted to assess ATP levels either in sampled extracellular fluids or in situ at the cell surface. Although our focus is on studies of ATP release from epit...
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PMID: 19685297
PDF is available here.
PDF is available here.
Abstract
Bioluminescence imaging (BLI) is frequently cited for its ease of quantification. This fundamental strength of BLI has led to applications in cancer research, cell transplantation, and monitoring of infectious disease in which bioluminescence intensity is correlated with other metrics. However, biol...
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PMID: 19685296
PDF is available here.
PDF is available here.
Abstract
We performed SA-CASSCF(12,12)/6-31G* and CASPT2(12,12)/6-31G*//SA-CASSCF(12,12)/6-31G* calculations to elucidate the reaction mechanism of bioluminescence from the firefly dioxetanone in the gas phase. Adiabatic transition state (TS) for the O-O bond cleavage and the minimum energy conical intersect...
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PMID: 18767834
PDF is available here.
PDF is available here.
Analytical Sciences (24)9 2008
Abstract
We determined the distribution coefficient, K, of the surfactants to the liposomal membrane. The result indicated that the surfactant with higher K value gives the maximal BL intensity at lower concentration....
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PMID: 18781021
PDF is available here.
PDF is available here.
Abstract
The inhibition mechanisms of the firefly luciferase (Luc) by the two major products of the reactions catalysed by Luc, oxyluciferin and dehydroluciferyl-adenylate (L-AMP), were investigated. Light production in the presence and absence of these inhibitors (0.5 to 2 microM oxyluciferin; 0.0025 to 1.2...
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PMID: 18754056
PDF is available here.
PDF is available here.
Abstract
The effects of osmolytes, including sucrose, sorbitol and proline on the remaining activity of firefly luciferase were measured. Heat inactivation studies showed that these osmolytes maintain the remaining activity of enzyme and increase activation energy of thermal unfolding reaction. Fluorescence...
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PMID: 18555523
PDF is available here.
PDF is available here.
Abstract
The glowworms of New Zealand and Australia are bioluminescent fly larvae that generate light to attract prey into their webs. Some species inhabit the constant darkness of caves as well as the dim, natural photophase of rain-forests. Given the diversity of light regimens experienced by glowworms in...
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PMID: 18663239
PDF is available here.
PDF is available here.
Biochemistry (New York) (73)8 2008
Abstract
Most firefly luciferases demonstrate a strong pH-dependence of bioluminescence spectra. Gene region encoding first 225 residues of Luciola mingrelica luciferase was subjected to random mutagenesis, and four mutants with altered pH-sensitivity of bioluminescence spectra were isolated. F16L substituti...
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PMID: 18774932
PDF is available here.
PDF is available here.
Abstract
We describe the cloning and identification of a nAChR modulator gene in an insect. This gene was isolated by searching the firefly Pyrocoelia rufa cDNA library, and the gene itself encodes a protein 120 amino acids in length, named Pr-lynx1. Pr-lynx1 shares all the features, including a cysteine-ric...
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PMID: 18455430
PDF is available here.
PDF is available here.
Transplantation (85)9 2008
Abstract
Noninvasive imaging modalities of functional islet mass, such as BLI (but not blood glucose levels), can prompt the appropriate timing of ALS treatment of islet allograft rejection and significantly prolong graft survival or protect the grafts from permanent loss....
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PMID: 18475179
PDF is available here.
PDF is available here.
Biochemical Journal (412)1 2008
Abstract
The bioluminescence colours of firefly luciferases are determined by assay conditions and luciferase structure. Owing to red light having lower energy than green light and being less absorbed by biological tissues, red-emitting luciferases have been considered as useful reporters in imaging technolo...
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PMID: 18251715
PDF is available here.
PDF is available here.
Abstract
We investigated this process in Japanese Luciola lateralis and L. cruciata by feeding males rhodamine B, a fluorescent dye known to stain spermatophore precursors. We then mated males with virgin females, and dissected pairs at various timepoints after mating. In both of these Luciola species, sperm...
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PMID: 18479701
PDF is available here.
PDF is available here.
Abstract
We used quantitative HTS, an approach that defines the concentration-response behavior of each library sample, to profile the ATP-dependent luciferase from Photinus pyralis against more than 70,000 samples. We found that approximately 3% of the library was active, containing only compounds with inhi...
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PMID: 18363348
PDF is available here.
PDF is available here.
Abstract
We report that larvae and pupae of the Brazilian fireflies Aspisoma lineatum and Cratomorphus sp emit a continuous weak glow throughout the entire body during all stages. This luminescence is especially developed after feeding, ecdysis and in the pupal stage, gradually disappearing as the cuticle be...
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PMID: 18385887
PDF is available here.
PDF is available here.
Abstract
The N(7)-methyl guanosine cap located on the 5'-terminus of mRNAs is important for a number of biochemical processes. A new dinucleoside triphosphate cap analog was synthesized with methyl groups on the N(7) of both guanine moieties, as well as the 3'-OH of one of the ribose moieties [see text]. The...
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PMID: 18178087
PDF is available here.
PDF is available here.
Abstract
Firefly luciferases are called pH-sensitive because their bioluminescence spectra display a typical red-shift at acidic pH, higher temperatures, and in the presence of heavy metal cations, whereas other beetle luciferases (click beetles and railroadworms) do not, and for this reason they are called...
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PMID: 18264583
PDF is available here.
PDF is available here.
Abstract
The results of the author's laboratory on the interaction of Luciola mingrelica firefly luciferase with substrates and their analogs using both steady-state and time resolved fluorescence are reviewed. The contribution of fluorescence of Trp and Tyr residues of the protein to its intrinsic fluoresce...
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PMID: 18264590
PDF is available here.
PDF is available here.
Abstract
Significant advances have occurred regarding our knowledge of firefly luciferase mechanisms. Although most of this progress was an outcome of molecular biology and structural studies, important achievements have also occurred on its fundamental chemistry. Those developments are here summarized and p...
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PMID: 18264582
PDF is available here.
PDF is available here.
EMBO Journal (27)1 2008
Abstract
We show that internalized proteins can have an alternative fate. After internalization, proteins are first unfolded to allow translocation into the cytosol using a pathway related to ER-associated degradation (ERAD). Subsequently the unfolded proteins can undergo cytosolic refolding assisted by the...
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PMID: 18046456
PDF is available here.
PDF is available here.
Entomology (53) 2008
Abstract
We review this recent research, which includes new phylogenetic results that shed light on the evolution of courtship signal diversity within the family Lampyridae, new insights into firefly flash control, and the discovery of firefly nuptial gifts. We present a comprehensive overview of sexual sele...
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PMID: 17877452
PDF is available here.
PDF is available here.
Abstract
Abstract
Positive-strand RNA viruses often use noncanonical strategies to usurp the host translational machinery for their own benefit. These strategies have been analyzed using transient expression assays in the absence of replication, with reporter genes replacing viral genes. A sensitive and convenient re...
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PMID: 18370250
PDF is available here.
PDF is available here.
Journal of Biotechnology (132)4 2007
Abstract
A DNA cartridge encoding Photinus pyralis luciferase (luc), lacZ homology extensions and an excisable marker was constructed to facilitate the conversion of Escherichia coli lacZ fusions to luc fusions by lambda Red-mediated recombination. This tool was used to transform a cspA::lacZ strain into a l...
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PMID: 17897748
PDF is available here.
PDF is available here.
ChemBioChem (8)17 2007
Abstract
We succeeded in developing modified siRNAs that have the greatest number of 4'-thioribonucleosides without loss of RNAi activity, and that exhibit potent RNAi activity against two target genes in three different cell lines. Our findings also indicate the significance of target sequences and cell lin...
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PMID: 17924376
PDF is available here.
PDF is available here.
Abstract
We usually use primer extension to map the transcription start site and a panel of PCR generated deletion mutants. This strategy is complex and time-consuming. Therefore, we decided to improve it by using Gateway and FLOE (Fluorescently Labeled Oligonucleotide Extension). In this report we developed...
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PMID: 17707908
PDF is available here.
PDF is available here.
American Naturalist (170)5 2007
Abstract
We have limited understanding of the relative importance of different costs. This study provides the first comprehensive assessment of signal costs for Photinus fireflies (Coleoptera: Lampyridae), using controlled experiments to measure both the energy and predation costs associated with their biolu...
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PMID: 17926292
PDF is available here.
PDF is available here.
Current Biology (17)19 2007
Abstract
Sexual signals are conspicuous and are typically assumed to be energetically costly, which keeps them honest. A recent study on fireflies has found that signal production is energetically cheap, but signalling remains expensive because of eavesdropping predators.
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PMID: 17925217
PDF is available here.
PDF is available here.
Abstract
We performed molecular phylogenetic analyses of species from cantharoid families, based on nucleotide sequence comparisons of nuclear 18S ribosomal DNA. The results shows that the luminous species Rhagophthalmus ohbai, which had sometimes been classified in Lampyridae, is excluded from a lampyrid cl...
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PMID: 17629422
PDF is available here.
PDF is available here.
Abstract
We use a phylogenetic approach to illuminate the relationships of North American lampyrids and the evolution of their mating signals. Specifically, to establish the first phylogeny of all North American lampyrid genera, we sequenced nuclear (18S) and mitochondrial (16S and COI) genes to investigate...
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PMID: 17644427
PDF is available here.
PDF is available here.
Carcinogenesis (28)9 2007
Abstract
We demonstrated that 3,3',4',5,5',7-hexahydroxyflavone (myricetin), one of the major flavonols in red wine, is a novel inhibitor of MEK1 activity and transformation of JB6 P+ mouse epidermal cells. Myricetin (10 microM) inhibited 12-O-tetradecanoylphorbol-13-acetate (TPA) or epidermal growth factor...
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PMID: 17693661
PDF is available here.
PDF is available here.
Biochemistry (New York) (72)9 2007
Abstract
Luciferase of the firefly Luciola mingrelica is characterized by fluorescence of not only the unique Trp residue (lambda(em) = 340 nm), but also that of Tyr residues (lambda(em) = 308 nm). Quenching of the intrinsic fluorescence of the luciferase by its substrates luciferin and ATP (AMP) has been st...
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PMID: 17922654
PDF is available here.
PDF is available here.
Abstract
We give the theoretically optimized structure of the excited state of oxyluciferin within luciferase, which gives luminescence calculated by the SAC-CI method that is close to the experimental value. Coulombic interactions with neighboring residues, in particular Arg218 and the phosphate group of AM...
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PMID: 17585760
PDF is available here.
PDF is available here.
Analytical Biochemistry (366)2 2007
Abstract
We selected novel mutations in Photinus pyralis luciferase with higher luminescence intensity. These included mutations at Ile423, Asp436, and Leu530. Luciferase is structurally composed of a large N-terminal active site domain (residues 1-436), a flexible linker (residues 436-440) peptide, and a sm...
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PMID: 17540326
PDF is available here.
PDF is available here.
Abstract
Journal of Immunology (178)11 2007
Abstract
We tested whether signaling skin cells via the NK1R promotes humoral and cellular immunity during skin genetic immunizations. We used the gene gun to deliver transgenic (tg) Ag to the skin of C57BL/6 mice and the selective NK1R agonist [Sar(9)Met (O(2)) (11)]-SP as a potential proinflammatory Th1-bi...
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PMID: 17513750
PDF is available here.
PDF is available here.
Abstract
Glycosylation of proteins can have a dramatic effect on their physical, chemical, and biological properties. Analogues of dihydrofolate reductase and firefly luciferase containing glycosylated amino acids at single, predetermined sites have been elaborated. Misacylated suppressor tRNAs activated wit...
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PMID: 17338522
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (282)12 2007
Abstract
We report here the construction and purification of a mutant at residue Arg(356), which is not conserved in beetle luciferases. By insertion of an additional residue (Arg(356)) using site-specific insertion mutagenesis in a green-emitter luciferase (Lampyris turkestanicus) the color of emitted light...
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PMID: 17197704
PDF is available here.
PDF is available here.
BioTechniques (42)3 2007
Abstract
Abstract
We describe the adaptation of two powerful genetic tools for use in B. burgdorferi. The first is a Photinus pyralis firefly luciferase gene reporter that was codon optimized to enhance translation in B. burgdorferi. Using this modified reporter, we demonstrated an increase in luciferase expression w...
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PMID: 17220265
PDF is available here.
PDF is available here.
Analytical Biochemistry (361)2 2007
Abstract
We present studies on the production of a set of thermostable red- and green-emitting luciferase mutants with bioluminescent properties suitable for dual-color reporter assays, biosensor measurements with internal controls, and imaging techniques. Starting with the luciferase variant Ser284Thr, we i...
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PMID: 17181991
PDF is available here.
PDF is available here.
Abstract
We experimentally manipulated matings of P. greeni fireflies to test the hypothesis that postcopulatory paternity success might be biased toward males that are more attractive during courtship interactions. We first measured male courtship attractiveness to individual females using field behavioral...
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PMID: 17348952
PDF is available here.
PDF is available here.
Luminescence (22)3 2007
Abstract
The term 'glowworm' is used in connection with the flightless females of lampyrid fireflies and to describe the luminescent larvae of certain fungus gnats that belong to the subfamilies Arachnocampinae, Keroplatinae and Macrocerinae of the dipteran family Keroplatidae. This review focuses on the lum...
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PMID: 17285566
PDF is available here.
PDF is available here.
Luminescence (22)1 2007
Abstract
Cationic liposomes containing phosphatidylcholine, cholesterol and distearyldimethylammonium chloride (DSDAC) enhanced maximum light emission (BL intensity) and total light emission from the firefly bioluminescence (BL) reaction. The increase in BL intensity was interpreted on the basis of the incre...
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PMID: 16871534
PDF is available here.
PDF is available here.
Abstract
We investigated whether PEGylated catalase (PEG-catalase, plasma half-life of 13.6 h) was able to prevent this after surgical removal of a footpad tumor in mice. Murine melanoma cells labeled with the firefly luciferase gene were used to monitor the distribution of tumor cells. After inoculation int...
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PMID: 17023272
PDF is available here.
PDF is available here.
Abstract
We found that firefly luciferase has fatty acyl-CoA synthetic activity, and also identified that the homolog of firefly luciferase in Drosophila melanogaster (CG6178) is a fatty acyl-CoA synthetase and is not a luciferase. In this study, we constructed chimeric proteins by exchanging the domain betw...
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PMID: 17090919
PDF is available here.
PDF is available here.
Biochemistry (Washington) (45)42 2006
Abstract
We examine the ability of calnexin to interact with a non-glycosylated substrate under physiological conditions of the ER lumen. We show that the soluble ER luminal domain of calnexin can indeed suppress the aggregation of non-glycosylated firefly luciferase at 37 degrees C and at the normal resting...
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PMID: 17042509
PDF is available here.
PDF is available here.
Abstract
We conclude that the neuroendocrine-specific expression of Chgb is mediated by the CRE and G/C boxes in cis and the transcription factors CREB, AP-2, Egr-1 and Sp1 in trans....
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PMID: 16987240
PDF is available here.
PDF is available here.
FEBS Letters (580)22 2006
Abstract
We show that the firefly produces both D- and L-luciferin, and that the amount of active D-luciferin increases gradually with maturation stage. Studies of firefly body extracts indicate the possible conversion of L-cysteine via L-luciferin into D-luciferin, suggesting that the biosynthesis is enzyma...
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PMID: 16979628
PDF is available here.
PDF is available here.
Journal of Natural Products (69)10 2006
Abstract
Eight new ent-beyerane metabolites, 5-8, 12, and 14-16, and four new ent-kaurane metabolites, 3, 10, 11, and 13, together with two known metabolites, 4 and 9, were isolated from the microbial transformations of steviol-16alpha,17-epoxide using Streptomyces griseus ATCC 10137 and Cunninghamella baini...
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PMID: 17067160
PDF is available here.
PDF is available here.
Abstract
We expect that this newly discovered luciferase will be suitable for a wide range of bioluminescence applications including in vivo imaging and multiplex assays....
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PMID: 16916615
PDF is available here.
PDF is available here.
Abstract
The cDNA of a firefly luciferase from lantern mRNA of Lampyroidea maculata has been cloned, sequenced and functionally expressed. The cDNA has an open reading frame of 1647 bp and codes for a 548-residue-long polypeptide. Noteworthy, sequence comparison as well as homology modeling showed the highes...
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PMID: 17002879
PDF is available here.
PDF is available here.
Analytical Biochemistry (356)1 2006
Abstract
We established a quantitative reporter gene protocol, the P/Rluc assay system, allowing the sequential measurement of Photinus and Renilla luciferase activities from the same extract. Other than comparable commercial reporter assay systems and their noncommercial counterparts, the P/Rluc assay syste...
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PMID: 16750160
PDF is available here.
PDF is available here.