Abstract
We compared the luminescence and catalytic properties of a recently cloned luciferase-like enzyme from Zophobas mealworm, an AMP/CoA-ligase displaying weak luminescence activity, with those of cloned luciferases from the three main families of luminescent beetles: Phrixthrix hirtus railroad worm; Py...
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PMID: 20526507
PDF is available here.
Parasitology (95 ( Pt 1))01 2009
Abstract
Several of the pathophysiological responses made by the beetle Tenebrio molitor, when infected with metacestodes of Hymenolepis diminuta, may be attributed to a parasite-induced reduction in host juvenile hormone titre. It has been suggested that production of this hormone by the corpora allata may...
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PMID: 3670902
PDF is available here.
Abstract
We present data that demonstrate that a coleopteran cadherin is a functional Cry3Aa toxin receptor. The Cry3Aa receptor cadherin was cloned from Tenebrio molitor larval midgut mRNA, and the predicted protein, TmCad1, has domain structure and a putative toxin binding region similar to those in lepido...
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PMID: 19416969
PDF is available here.
Abstract
Numerous data of long-term investigations of weak agents on morphogenetic processes in invertebrates (regeneration of planarians, Dugesia (Girardia) tigrina and postembryonic development of insects, the grain beetle Tenebrio molitor) have been analyzed. Weak physical and chemical factors were used:...
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PMID: 20067193
PDF is available here.
Abstract
Elevated tolerance to UV-B radiation and heat may be induced in conidia produced on fungi exposed during mycelial growth to sublethal stresses other than heat or UV-B. This is due to a phenomenon referred to as 'cross-protection'. Several mechanisms are associated with this increased conidial tolera...
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PMID: 18938068
PDF is available here.
Abstract
These results demonstrate that the germination, adhesion and virulence of M. anisopliae conidia can be strongly influenced by culture conditions (including stresses) during production of the conidia....
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PMID: 18947989
PDF is available here.
Abstract
Nettie M. Stevens is one of the first women who contributed to genetic research. She discovered the role of sex chromosomes in sex determination during the first decade of the twentieth century. However, her discovery has rarely been recognized, and her scientific reputation was eclipsed by that of...
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PMID: 18950586
PDF is available here.
Hongnan Kan,
Chan-Hee Kim,
Hyun-Mi Kwon,
Ji-Won Park,
Kyung-Baeg Roh,
Hanna Lee,
Bum-Joon Park,
Rong Zhang,
Jinghai Zhang,
Kenneth Söderhäll,
Nam-Chul Ha and
Bok Luel Lee
Abstract
We provide biochemical evidence that the Tenebrio molitor Spätzle-processing enzyme converts both the 79-kDa Tenebrio prophenoloxidase and Tenebrio clip-domain SPH1 zymogen to an active melanization complex. This complex, consisting of the 76-kDa Tenebrio phenoloxidase and an active form of Tenebri...
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PMID: 18628205
PDF is available here.
Abstract
Biological activities of crustacean cardioactive peptide (CCAP; PFCNAFTGCa) and leucomyosuppressin (Lem-MS; pQDVDHVFLRFa) were studied in heterologous bioassays in the larvae and adults of Tenebrio molitor. CCAP exerted a reversible and dose-dependent cardio-stimulatory effect on the semi-isolated h...
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PMID: 18521612
PDF is available here.
Abstract
Our results brought until then unknown original findings that insects, represented here by the T. molitor species, use-in contrast to another previously researched Arthropod, spiny lobster-the inclination compass....
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PMID: 18404256
PDF is available here.
Abstract
We measured delta(13)C(breath) in fasted and recently fed insectivorous Myotis myotis (Chiroptera) to assess the residence time of carbon isotopes in the pool of metabolized substrate, and whether delta(13)C(breath) in satiated individuals levels off at values similar to the dietary isotope signatur...
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PMID: 18587117
PDF is available here.
Abstract
We performed the synthesis of the following peptides: a native decapeptide from Manduca sexta Mas-MT-I and its 11 analogs with shortened sequences at the N-end as well as a growth suppressor, a pentapeptide isolated from Antheraea yamamai, Any-GS and its 10 analogs, modified at position 1 and with a...
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PMID: 18181232
PDF is available here.
Abstract
We found that antimicrobial activity in haemolymph increased rapidly during the first 48h after a challenge and was maintained at high levels for at least 14 days. There was no difference in the magnitude of responses to live or dead Escherichia coli or Bacillus subtilis. While peptidoglylcan also e...
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PMID: 18513740
PDF is available here.
Abstract
We conducted a series of laboratory experiments on birds captured during the autumn migration period in which we tested the hypotheses that drinking water increases food use by easing limitations on the birds' dietary choices and, consequently, feeding and food processing rates, and that the availab...
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PMID: 18278515
PDF is available here.
Abstract
The lack of available cell lines has hampered the study of viral diseases in crustaceans. This is particularly important for aquaculture which has been plagued by viral diseases since its rapid expansion to meet with the growing demand for seafood products. This study was designed to find an alterna...
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PMID: 18191141
PDF is available here.
Chan-Hee CH Kim,
Su-Jin SJ Kim,
Hongnan H Kan,
Hyun-Mi HM Kwon,
Kyung-Baeg KB Roh,
Rui R Jiang,
Yu Y Yang,
Ji-Won JW Park,
Hyeon-Hwa HH Lee,
Nam-Chul NC Ha,
Hee Jung HJ Kang,
Masaru M Nonaka,
Kenneth K Söderhäll and
Bok Luel BL Lee
Abstract
We purified and cloned a novel upstream serine protease of SPE that we named SAE, SPE-activating enzyme, from the hemolymph of a large beetle, Tenebrio molitor larvae. This enzyme was activated by Tenebrio modular serine protease and in turn activated the Tenebrio SPE. The biochemical ordered functi...
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PMID: 18195005
PDF is available here.
Abstract
We have examined the effects of the Led-NPF-I peptide (Ala-Arg-Gly-Pro-Gln-Leu-Arg-Leu-Arg-Phe-amide) and a series of ten analogues on the heart contractile activity of Tenebrio molitor and Zophobas atratus, and the structure-activity relationships for cardioactive action of Led-NPF-I were establish...
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PMID: 17929328
PDF is available here.
Irina A Goptar,
Irina Yu Filippova,
Elena N Lysogorskaya,
Elena S Oksenoit,
Konstantin S Vinokurov,
Dmitry P Zhuzhikov,
Natalja V Bulushova,
Igor A Zalunin,
Yakov E Dunaevsky,
Mikhail A Belozersky,
Brenda Oppert and
Elena N Elpidina
Abstract
Two soluble post-proline cleaving peptidase activities, PPCP1 and PPCP2, were demonstrated in Tenebrio molitor larval midgut with the substrate benzyloxycarbonyl-L-alanyl-L-proline p-nitroanilide. Both activities were serine peptidases. PPCP1 was active in acidic buffers, with maximum activity at pH...
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PMID: 18067867
PDF is available here.
Abstract
Linear diterpenes isolated from aerial parts of Baccharis thymifolia ( 1- 3) were tested for insect growth inhibitory activity against Tenebrio molitor larvae. Compounds 4- 16 were prepared by various chemical transformations. The diterpenes 6,10-( E,E)-thymifodioic acid ( 1), the butenolide 3, and...
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PMID: 18232640
PDF is available here.
Abstract
Four neuropeptides were identified from the brain and corpora cardiaca-corpora allata (CC-CA) of the mealworm beetle Tenebrio molitor using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and information derived from the genome of the red flour beetle, Tri...
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PMID: 18201799
PDF is available here.
Abstract
The strong magnetic field influence characteristics of protocerebral neurosecretory neurons and corpora allata in the late Tenebrio molitor pupae....
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PMID: 18246478
PDF is available here.
Abstract
A cDNA coding for a Tenebrio molitor midgut protein named peritrophic membrane ancillary protein (PMAP) was cloned and sequenced. The complete cDNA codes for a protein of 595 amino acids with six insect-allergen-related-repeats that may be grouped in A (predicted globular)- and B (predicted nonglobu...
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PMID: 18207083
PDF is available here.
Abstract
This study presents the first characterization of a WD40 repeat-containing myosin identified in the apicomplexan parasite Gregarina polymorpha. This 222.7 kDa myosin, GpMyoF, contains a canonical myosin motor domain, a neck domain with 6 IQ motifs, a tail domain containing short regions of predicted...
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PMID: 18372636
PDF is available here.
Abstract
Factors that influence the localized abundance and distribution of lesser mealworm, Alphitobius diaperinus (Panzer), in litter of two compacted earth-floor broiler houses in subtropical Australia were studied using various experimental manipulations. Numbers of lesser mealworms substantially increas...
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PMID: 18330117
PDF is available here.
Abstract
This paper presents results on isolates of the entomopathogenic fungus Beauveria bassiana for Alphitobius diaperinus (Coleoptera: Tenebrionidae) control. Of the 30 isolates tested, four (CG 71, CG 152, UNIOESTE 4, and UNIOESTE 40) resulted in mortality rates >or= 40% within 10 days. These mortality...
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PMID: 17869265
PDF is available here.
Abstract
Two post-proline cleaving enzymes PRE1 and PRE2 with molecular masses of 101 and 62 kDa, respectively, capable of hydrolyzing Z-AlaAlaPro-pNA were isolated for the first time from the midgut of the flour beetle Tenebrio molitor and characterized. PRE1 is active only in acidic media, with a maximum a...
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PMID: 18672677
PDF is available here.
Abstract
We compared the flight and echolocation behaviour of a vespertilionid bat (Myotis nattereri) approaching a large stationary or a small moving target. Bats were trained to either land on a landing grid or to catch a moving tethered mealworm. When closing in on these two targets, the bats emitted grou...
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PMID: 18055634
PDF is available here.
Abstract
Repeated subculturing caused rapid changes in the spore surface properties and virulence of Metarhizium anisopliae. Of the two strains evaluated, M. anisopliae V245 attenuated more rapidly than V275. Electrophoretic mobility and Radial Flow Chamber assays were used for the first time to generate qua...
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PMID: 17937664
PDF is available here.
Abstract
We show that in the yellow mealworm, Tenebrio molitor, septic injuries induce the synthesis of N-beta-alanyldopamine (NBAD), which is known as the main sclerotization precursor of insect brown cuticles. We demonstrate that NBAD synthase is induced in the epidermis of the mealworm and of the Medfly,...
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PMID: 17706245
PDF is available here.
Abstract
The objective of this study was to identify midgut microvillar proteins in insects appearing earlier (Coleoptera) and later (Lepidoptera) in evolution. For this, cytoskeleton-free midgut microvillar membrane from Spodoptera frugiperda (Lepidoptera) and Tenebrio molitor (Coleoptera) were used to rais...
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PMID: 17644107
PDF is available here.
Abstract
We show that predators that have learned to avoid chemically defended prey continue to attack defended individuals at levels determined by their current toxin burden. European starlings learned to discriminate between sequentially presented defended and undefended mealworms with different color sign...
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PMID: 17716896
PDF is available here.
Abstract
During sclerotization of insect cuticle the acyldopamines, N-acetyldopamine (NADA) and N-beta-alanyldopamine (NBAD), are oxidatively incorporated into the cuticular matrix, thereby hardening and stabilizing the material by forming crosslinks between the proteins in the cuticular matrix and by formin...
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PMID: 17681236
PDF is available here.
Abstract
The beetle-tapeworm life cycle provides a convenient system to study how host behaviour influences the probability of re-infection because initial and secondary infections can be tracked. The beetle, Tenebrio molitor, is infected with the tapeworm Hymenolepis diminuta when it ingests rat faeces cont...
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PMID: 17875228
PDF is available here.
Abstract
We hypothesize that they play an important role in support of their hosts. However, studies done to date on the impact of gregarines on their host are conflicting. Therefore, we studied the impact of gregarines on their host using a model Gregarina niphandrodes infection in Tenebrio molitor. The imp...
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PMID: 17716459
PDF is available here.
Abstract
Peptidase sequences were analysed in randomly picked clones from cDNA libraries of the anterior or posterior midgut or whole larvae of the yellow mealworm, Tenebrio molitor Linnaeus. Of a total of 1528 sequences, 92 encoded potential peptidases, from which 50 full-length cDNA sequences were obtained...
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PMID: 17651235
PDF is available here.
Abstract
We identified four proteins of this family in Anopheles gambiae that we have named CPF. We have also identified CPFs from other insects by searching databases. Alignment of these CPF proteins showed that the conserved region is only 44 aa long and revealed another conserved motif at the C-terminus....
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PMID: 17550824
PDF is available here.
Abstract
We describe the ultrastructure of the syzygy junction and the nucleus during the transition from unassociated trophozoites to paired gamonts to gamonts in syzygy. Throughout this process, the folds within the syzygy junction undergo changes that correspond to changes of the epicytic folds. The nucle...
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PMID: 17626337
PDF is available here.
Abstract
The surface architecture of oocysts produced by Gregarina niphandrodes (Eugregarinorida) from Tenebrio molitor adults (Coleoptera: Tenebrionidae) as revealed by scanning electron microscopy is reported. Gametocysts were allowed to dehisce on 15-mm, round cover glasses; the cover glasses with their o...
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PMID: 17626372
PDF is available here.
Abstract
We introduced different densities of mealworms (Tenebrio molitor), an alternative prey, to a previously studied predator-prey system in which paper wasps (Polistes dominulus) preyed on shield beetle larvae (Cassida rubiginosa). We tested if alternative prey affects predation on the first prey (i.e.,...
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PMID: 17536415
PDF is available here.
Abstract
Knowledge of the population fluctuation and spatial distribution of pests is fundamental for establishing an appropriate control method. The population fluctuation and spatial distribution of the Alphitobius diaperinus in a poultry house in Cascavel, in the state of Parana, Brazil, was studied betwe...
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PMID: 17876430
PDF is available here.
Abstract
This paper reports the effects of different diuretic factors on the Malpighian tubules of beetles. Calcitonin (CT)-like peptides from silkmoth and mosquito increase fluid secretion in a dose-dependent manner in the tubules of Tenebrio molitor, but the cockroach CT-like peptide, Dippu-DH(31), has no...
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PMID: 17292388
PDF is available here.
Abstract
Our results indicate that both peptides have a very similar mode of action despite the differences in their amino acid sequence. They seem to interfere with vitellogenin production by the fat body as well as with vitellogen uptake by the oocytes through modification of patency....
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PMID: 17294425
PDF is available here.
Abstract
We describe here the preparation of a molecular weight standard from a completely different DNA source - the uniquely organized genome of the beetle Tenebrio molitor. The standard can be used to accurately size DNAs between 150 and 4500 bp, a useful range of sizes for many agarose gel electrophoresi...
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PMID: 17309053
PDF is available here.
Abstract
We have quantitated the decrease in cuticular N-terminal amino groups and lysine epsilon-amino groups during the first 24h of sclerotization in adult locusts, Schistocerca gregaria, and in larval and adult beetles, Tenebrio molitor, as well as the increase in beta-alanine amino groups in Tenebrio cu...
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PMID: 17296497
PDF is available here.
Abstract
We describe a proteomic approach for the identification of bioactive alpha-AIs from wheat and, in particular, a 3-D technique that allows to best identify and characterize the dimeric fraction. The technique takes advantage of the thermal resistance of alpha-AIs (resistant to T > 70 degrees C) and c...
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PMID: 17203506
PDF is available here.
Abstract
We analysed the mating behaviour of pairs of Tenebrio molitor beetles under three odour treatments representing increasing levels of sperm competition risk (SCR) and sperm competition intensity (SCI). Copula duration and male and female post-copulatory behaviour varied significantly with odour treat...
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PMID: 17148255
PDF is available here.
Abstract
We tested the effects of complementary resources on dietary shifts of an omnivorous mammal, the white-footed mouse Peromyscus leucopus, in the context of depletable food patches in the natural environment. We used two complementary resources: seeds that provide a higher energy gain per unit handling...
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PMID: 17489457
PDF is available here.
Abstract
The entomopathogenic nematode-bacteria complexes Heterorhabditis bacteriophora/Photorhabdus luminescens and Steinernema carpocapsae/Xenorhabdus nematophila are mass produced for use as biological insecticides. Stability of the bacterial partner in culture is essential for maintaining traits importan...
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PMID: 16941119
PDF is available here.
Abstract
Soaking L1 stage H. bacteriophora with seven dsRNAs of genes whose C. elegans orthologs had severe RNAi phenotypes resulted in highly penetrant and obvious developmental and reproductive abnormalities. The efficacy of postembryonic double strand RNA interference (RNAi) was evident by abnormal gonad...
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PMID: 17803822
PDF is available here.
Abstract
We tried to determine whether gregarines possess a plastid and/or its genome. We used PCR and dot-blot hybridization to determine whether the gregarine Gregarina niphandrodes possesses a plastid genome. We used an inhibitor of plastid function for any reduction in gregarine infection, and transmissi...
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PMID: 17300522
PDF is available here.
Abstract
Early developmental stages of Gregarina steini Berndt, 1902 from the intestine of Tenebrio molitor larvae were studied by transmission electron microscopy. The formation and structure of the eugregarine attachment site were compared with comparable features found on the feeder organelle of Cryptospo...
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PMID: 18070327
PDF is available here.
Abstract
We hypothesized that CO(2) production from an insect changes after it is infected, and that these changes could influence nematode infection decisions. Changes in CO(2) released by two insect species (Galleria mellonella and Tenebrio molitor) after infection by one of four nematode species (Steinern...
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PMID: 17054978
PDF is available here.
Abstract
The partial cDNA sequence coding for the antifreeze proteins in the Tenebrio molitor was obtained by RT-PCR. Sequence analysis revealed nine putative cDNAs with a high degree of homology to Tenebrio molitor antifreeze proteins. The recombinant pGEX-4T-1-tmafp-XJ430 was introduced into E. coli BL21 t...
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PMID: 17138539
PDF is available here.
Abstract
We show empirically that this assumption is correct. Invertebrate innate immunity relies heavily on effector systems which, on activation, produce cytotoxins that kill pathogens. Reliance on these robust, fast-acting, generic killing mechanisms ensures a potent and rapid response to pathogen invasio...
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PMID: 16959651
PDF is available here.
Abstract
The spectrum of Tenebrio molitor larval digestive proteinases was studied in the context of the spatial organization of protein digestion in the midgut. The pH of midgut contents increased from 5.2-5.6 to 7.8-8.2 from the anterior to the posterior. This pH gradient was reflected in the pH optima of...
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PMID: 16859942
PDF is available here.
Abstract
Tenebrio molitor larval digestive proteinases were purified and characterized by gel filtration chromatography combined with activity electrophoresis. Cysteine proteinases, consisting of at least six distinct activities, were found in three chromatographic peaks in anterior and posterior midgut chro...
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PMID: 16926103
PDF is available here.
Abstract
Insect chitinases are involved in degradation of chitin from the exoskeleton cuticle or from midgut peritrophic membrane during molts. cDNAs coding for insect cuticular and gut chitinases were cloned, but only chitinases from moulting fluid were purified and characterized. In this study the major di...
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PMID: 17027845
PDF is available here.
Abstract
We present a new protocol for production of fully folded and active T. molitor thermal hysteresis protein in bacteria, without the need for in vitro refolding. The protein coding sequence was fused to those of various carrier proteins and expressed at low temperature in a bacterial strain specially...
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PMID: 16542851
PDF is available here.
Abstract
Metacestodes of Hymenolepis diminuta secrete a molecule that decreases vitellogenin (Vg) synthesis in the beetle host, Tenebrio molitor. The 5608 bp T. molitor Vg cDNA represents a single-copy gene encoding a single open reading frame of 1821 amino acids with a predicted molecular mass of 206 kDa. N...
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PMID: 16907836
PDF is available here.