Abstract
We utilized RNA interference (RNAi) and microarray to study the function of the bursicon receptor (Tcrk) in the model insect, Tribolium castaneum. The data included here showed that in addition to cuticle tanning and wing expansion reported previously, Tcrk is also required for development and expan...
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PMID: 20457145
PDF is available here.
Abstract
Data from long-term Tribolium castaneum (Herbst) pheromone trapping programs in two flour mills was used to evaluate the impact of structural fumigations (n = 23) on pest populations. The two mills differed in mean number of beetles captured and proportion of traps with captures of one or more beetl...
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PMID: 20568648
PDF is available here.
Abstract
Structural fumigations of food processing plants to manage stored-product insects has been a major component of pest management programs, but limited information on field efficacy is available. Efficacy, based on pheromone trapping data, consists of initial reduction in captures after treatment and...
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PMID: 20568649
PDF is available here.
Abstract
With its recently sequenced genome, the red flour beetle Tribolium castaneum became one of the few model organisms with all the main genetic tools. As a coleoptera, it belongs to the most species-rich order of animals. Tribolium is also a worldwide pest for stored dried foods. Regarding developmenta...
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PMID: 20346280
PDF is available here.
Abstract
Querying the genome of the red flour beetle, Tribolium castaneum, with the Drosophila melanogaster Yellow-y (DmY-y) protein sequence identified 14 Yellow homologs. One of these is an ortholog of DmY-y, which is required for cuticle pigmentation (melanization), and another is an ortholog of DmY-f/f2,...
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PMID: 20149870
PDF is available here.
Abstract
We studied the gene family encoding CTLPs in the genome of the red flour beetle, Tribolium castaneum. Using an extended search pattern, we identified 14 TcCTLP genes that encode peptidases with S1 specificity pocket residues typically found in chymotrypsin-like enzymes. We further analyzed the expre...
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PMID: 19897036
PDF is available here.
Abstract
We used quantitative RT-PCR to verify that TH expression occurs at the time of cuticle tanning and immunohistochemistry to confirm that TH is expressed in the epithelial cells underlying sclerotized cuticle. In addition, we found that a reduction in TH function (mediated by RNA interference) resulte...
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PMID: 20080183
PDF is available here.
Abstract
We propose the name "Peritrophic Matrix Proteins" (PMP) for this family. The second family contains eight proteins encoded by seven genes (one gene codes for 2 splice variants), which are closely related to gasp/obstructor-like proteins that contain 3 ChtBD2s each. The third family has ten proteins...
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PMID: 20144715
PDF is available here.
Abstract
To investigate gene expression in Tribolium castaneum exposed to Beauveria bassiana, reference genes for qPCR were evaluated. Of these, beta-actin, alpha-tubulin, and RPS6 were not stable. The most stable were ribosomal protein genes, RPS3, RPS18, and RPL13a. Syntaxin1, syntaxin6, and E-cadherin may...
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PMID: 20026205
PDF is available here.
Abstract
In our screening program for new agrochemicals from Chinese medicinal herbs, Murraya exotica was found to possess insecticidal activity against the maize weevil, Sitophilus zeamais and red flour beetle, Tribolium castaneum. The essential oil of aerial parts of M. exotica was obtained by hydrodistill...
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PMID: 20736910
PDF is available here.
Abstract
With the established GAL4/UAS binary expression system, ectopic misexpression approaches are now feasible in Tribolium. Our results support the contention that high-level transgene expression usually requires endogenous regulatory sequences, including endogenous core promoters in Tribolium and proba...
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PMID: 20482875
PDF is available here.
Abstract
We have taken advantage of the recently acquired T. castaneum genome to identify T. castaneum genes and proteins in one of the more critical environmental interfaces of the insect, the larval alimentary tract. Genetic transcripts isolated from the T. castaneum larval gut were labeled and hybridized...
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PMID: 19545177
PDF is available here.
Parasitology (95 ( Pt 1))01 2009
Abstract
Biological characteristics of infectivity, growth rate and fecundity of Hymenolepis diminuta isolated from wild Rattus rattus in Japan were compared with parasites of Texas origin maintained for several generations in this and many other laboratories in laboratory bred Rattus norvegicus. The timing...
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PMID: 3670903
PDF is available here.
Abstract
We have undertaken attempts to establish methods of detection of T. confusum based on molecular biology techniques of standard and real-time polymerase chain reaction (PCR). Total DNA of T. confusum and red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae), used as a negative co...
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PMID: 19736785
PDF is available here.
Abstract
We used custom microarrays and identified 112 genes that were highly expressed in male accessory glands (MAG) in the red flour beetle, Tribolium castaneum. Out of these 112 identified genes, 59 of them contained sequences coding for signal peptide and cleavage site and the remaining 53 contained tra...
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PMID: 19324087
PDF is available here.
Parasitology (90 ( Pt 3))03 2009
Abstract
This study was based on the experimental exposure of beetles to homologous and heterologous infections of Hymenolepis diminuta and Raillietina cesticillus. The results demonstrated that, for both species over the range of parasite densities employed, the presence of a primary infection had no effect...
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PMID: 4040235
PDF is available here.
Parasitology (92 ( Pt 2))02 2009
Abstract
Some effects of Hymenolepis diminuta on the fecundity of Tribolium confusum are described. Host fecundity is observed to be reduced exponentially with increasing parasite burden/host, although there are differences in the ability of individual hosts to respond to parasitism. Of particular interest i...
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PMID: 3714304
PDF is available here.
Abstract
We infer from these results that a tradeoff between heat resistance, registered as hsp83 expression, and ovarian development operates under heat stress in T. castaneum.
(c) 2009 Wiley Periodicals, Inc....
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PMID: 19194986
PDF is available here.
Abstract
The effects of the monoterpenes of 3-carene, 1,8-cineole, beta-pinene, terpinene and terpinolene on the repellent, contact toxicity of Tribolium castaneum adults and sustaining fumigant toxicity of Sitophilus zeamaise adults were examined. Monoterpene of 1,8-cineole in 20 microL mL(-1) and beta-pine...
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PMID: 19662572
PDF is available here.
Abstract
We have addressed the question of the segmental affinity of the hypopharyngeal lobes by conducting a detailed comparison of gene expression patterns between Drosophila and the red flour beetle Tribolium castaneum, in which the intercalary segment is unambiguously marked out by lab. We demonstrate th...
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PMID: 19196336
PDF is available here.
Abstract
A recent paper in BMC Biology reports the first large-scale insertional mutagenesis screen in a non-drosophilid insect, the red flour beetle Tribolium castaneum. This screen marks the beginning of a non-biased, 'forward genetics' approach to the study of genetic mechanisms operating in Tribolium.
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PMID: 20053302
PDF is available here.
Abstract
We report the first isolation and characterization of insect fatty acid Delta12-desaturase genes, AdD12Des from house cricket (Acheta domesticus) and TcD12Des from the red flour beetle (Tribolium castaneum), responsible for the production of linoleic acid from oleic acid. Sequence analysis shows the...
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PMID: 19133076
PDF is available here.
Abstract
Our study presents an intriguing example how the evolution of multigene families may contribute to the complexity of the innate immune response....
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PMID: 18703524
PDF is available here.
Abstract
We found a small percentage of embryos lacking abdominal segments. Additional removal of Tc-Wnt1 significantly enhanced the penetrance of this phenotype. Seeking alternative methods to deplete Wnt signal, we performed RNAi with other components of the Wnt pathway including wntless (wls), porcupine (...
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PMID: 18926702
PDF is available here.
Abstract
We present the high-resolution structure of the Tribolium castaneum catalytic subunit of telomerase, TERT. The protein consists of three highly conserved domains, organized into a ring-like structure that shares common features with retroviral reverse transcriptases, viral RNA polymerases and B-fami...
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PMID: 18758444
PDF is available here.
Abstract
Ecdysteroids regulate insect growth and development through a heterodimeric complex of nuclear receptors consisting of ecdysone receptor (EcR) and ultraspiracle (USP). In the red flour beetle, Tribolium castaneum, two isoforms each of EcR and USP have been identified. Quantitative real-time reverse-...
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PMID: 18583027
PDF is available here.
Abstract
Two polyclonal antibodies (Pab) were developed for the detection of Tribolium castaneum, which is a stored product pest of medical and economical importance. Selected Pab anti- T. castaneumK51 showed low cross-reactivity to other stored product arthropods but revealed high reactivity to T. destructo...
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PMID: 18698788
PDF is available here.
Abstract
We describe here the single Tribolium ortholog of the Drosophila genes knirps and knirps-related (called Tc-knirps). Tc-knirps expression is conserved during head patterning and at later stages. However, posterior Tc-knirps expression in the ectoderm is limited to a stripe in A1, instead of a broad...
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PMID: 18586236
PDF is available here.
Abstract
We applied the joint scaling principle to assess epistasis and other nonadditive genetic components of two resistance traits, survival, and parasite spore load, in population crosses of the red flour beetle Tribolium castanaeum under infections with the microsporidian Nosema whitei. We found nonaddi...
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PMID: 18564375
PDF is available here.
Abstract
We identified 12 Tubulin family members from the genome sequence of the red flour beetle, Tribolium castaneum, and used the promoter from one of these to drive constitutive expression of a transgene. The activity of the T. castaneum alpha-Tubulin1 (TcalphaTub1) putative promoter was pre-tested in co...
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PMID: 18625397
PDF is available here.
Abstract
We show that Medea(1) activity in Tribolium castaneum is associated with a composite Tc1 transposon inserted just downstream of the neurotransmitter reuptake symporter bloated tubules (blot), whose Drosophila ortholog has both maternal and zygotic functions. The 21.5-kb insertion contains defective...
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PMID: 18621706
PDF is available here.
Abstract
We derive a family of stochastic Ricker models using different combinations of all these stochastic factors, and show that extinction risk depends strongly on the combination of factors that contribute to stochasticity. Furthermore, we show that only with the full stochastic model can the relative i...
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PMID: 18596809
PDF is available here.
Abstract
We studied the 'crowding effect' in Tribolium confusum infected with Hymenolepis diminuta. Manipulations included age and number of parasites, and diet, sex, age and number of exposures of hosts. Volume per parasite was unaffected until an intensity of at least 5-10 parasites per host, then declined...
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PMID: 18474124
PDF is available here.
Abstract
We investigated the role of TcMet in JH action during larval-pupal metamorphosis. The phenotypes of TcMet RNAi insects shared similarity with the phenotypes of some allatectomized lepidopteran larvae that were attempting to undergo precocious larval-pupal metamorphosis. Knocking-down TcMet during th...
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PMID: 18450431
PDF is available here.
Abstract
We have demonstrated that it has in vivo diuretic activity, but that it has no direct effect on Malpighian tubules. However, when the central nervous system plus corpora cardiaca and corpora allata are incubated along with the peptide and Malpighian tubules, the latter are stimulated by the AVPL pep...
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PMID: 18549960
PDF is available here.
Abstract
Beetles are reckoned to make up about one quarter of animal species. Now, the first genome of a beetle--the red flour beetle Tribolium castaneum, a pest and developmental model system alike--has been sequenced.
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PMID: 18522815
PDF is available here.
Abstract
Commercial food- and pheromone-baited pitfall traps and pheromone-baited sticky traps were used during 2003 to survey stored-product insect adults in eight participating feed mills in the midwestern United States. Across the eight feed mills, 27 species of beetles (Coleoptera) and three species of m...
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PMID: 18613605
PDF is available here.
Abstract
This study was designed to explore the nutritional relationship between Gregarina confusa (Apicomplexa: Eugregarinida) parasites and its coleopteran host, Tribolium destructor, by measuring the cytoplasmic density of gregarines in continuously fed larvae, starved larvae, and larvae refed after starv...
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PMID: 18605781
PDF is available here.
Abstract
Structural heat treatment, a viable alternative to methyl bromide fumigation, involves raising the ambient temperature of food-processing facilities between 50 and 60 degrees C by using gas, electric, or steam heaters, and holding these elevated temperatures for 24 h or longer to kill stored-product...
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PMID: 18613604
PDF is available here.
Abstract
Barley, Hordeum vulgare L., one of the important crops in Canada, is used in malting, feed, and food industries. Disinfestation of barley using microwaves can be an alternative to chemical methods used to kill insects. A pilot-scale industrial microwave system operating at 2.45 MHz was used in this...
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PMID: 18613607
PDF is available here.
Abstract
We asked whether the function of their orthologs is conserved in the red flour beetle Tribolium castaneum which in contrast to Drosophila develops its larval head in a way typical for insects. We find that depending on dsRNA injection time, two functions of Tc-orthodenticle1 (Tc-otd1) can be identif...
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PMID: 18407258
PDF is available here.
Abstract
The biological functions of individual members of the large family of chitinase-like proteins from the red flour beetle, Tribolium castaneum (Tc), were examined by using gene-specific RNAi. One chitinase, TcCHT5, was found to be required for pupal-adult molting only. A lethal phenotype was observed...
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PMID: 18436642
PDF is available here.
Abstract
Sexual selection is a major force driving the evolution of diverse reproductive traits. This evolutionary process is based on individual reproductive advantages that arise either through intrasexual competition or through intersexual choice and conflict. While classical studies of sexual selection f...
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PMID: 18429767
PDF is available here.
Abstract
I discuss these differences in relation to divergent and convergent changes in cellular embryology. I speculate on how segmentation gene networks have evolved to operate in divergent embryological contexts, and highlight the role that co-option might have played in this process. I argue that insects...
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PMID: 18192180
PDF is available here.
Abstract
We characterized the proliferation of ISCs during midgut remodeling in the red flour beetle, Tribolium castaneum. Midgut remodeling is initiated at 96 hr after ecdysis into the final instar larval stage. Immunocytochemistry with bromodeoxyuridine and phospho-histone H3 antibodies showed that the ISC...
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PMID: 18297733
PDF is available here.
Abstract
We have analysed the expression and function of the Tribolium brachyenteron ortholog (Tc-byn). During embryogenesis, Tc-byn is exclusively expressed in the growth zone of the extending germband and later becomes confined to the distal proctodeum and the hindgut, a situation that parallels the expres...
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PMID: 18392878
PDF is available here.
Abstract
We found a total of nine Wnt genes. In addition to wg or Wnt1, Tribolium contains orthologs of the vertebrate Wnt5-7 and Wnt9-11 genes. As in Drosophila, Wnt1, Wnt6 and Wnt10 are clustered in the genome. Comparative genomics indicates that Wnt9 is also a conserved member of this cluster in several i...
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PMID: 18392880
PDF is available here.
Abstract
We analyzed the components of TGFbeta-signaling present in the genome of the beetle Tribolium castaneum. We were able to identify orthologs of the BMPs Decapentaplegic and Glass bottom boat, of the Activins Activinbeta and Dawdle, as well as orthologs of the less well-known ligands Myoglianin and Ma...
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PMID: 18392881
PDF is available here.
Abstract
Insect embryogenesis is best understood in the fruit fly Drosophila. However, Drosophila embryogenesis shows evolutionary-derived features: anterior patterning is controlled by a highly derived Hox gene bicoid, the body segments form almost simultaneously and appendages develop from imaginal discs....
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PMID: 18392874
PDF is available here.
Abstract
We identified four Fgf family members representing three subfamilies. Tribolium has Fgf1 genes that are absent in Drosophila but known from vertebrates. By phylogenetic analysis and microsynteny to Drosophila, we further classify Tc-fgf 8 as an ancestor of pyramus and thisbe, the fly Fgf8 genes. Tc-...
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PMID: 18392877
PDF is available here.
Abstract
We have compiled and analyzed all available expressed sequence tag (EST) data. The raw data consist of 61,228 ESTs, including 10,704 obtained from NCBI and an additional 50,524 derived from 32,544 clones generated in our laboratories. These sequences were amassed from cDNA libraries representing six...
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PMID: 18342244
PDF is available here.
Abstract
Nuclear receptors (NRs) are classified by the presence of a well-conserved DNA-binding domain and a less conserved ligand-binding domain and function as key control points in diverse signaling and metabolic pathways. NRs are switched on and off by small molecule ligands with properties similar to in...
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PMID: 18342248
PDF is available here.
Abstract
I enzymes include CDA1 and isoforms of CDA2, each containing in addition to a polysaccharide deacetylase-like catalytic domain, a chitin-binding peritrophin-A domain (ChBD) and a low-density lipoprotein receptor class A domain (LDLa). Group II is composed of CDA3 orthologs from each insect species w...
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PMID: 18342249
PDF is available here.