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Euryarchaeota (8):
Recent article
Euryarchaeota
Abstract
We investigated the abundance, community structure, and diversity of Bacteria and Archaea along these gradients to further distinguish the ecologies of these domains outside their established physiological range. Quantitative PCR was used to enumerate 16S rRNA gene abundances of Bacteria, Archaea, a...
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PMID: 19948847
PDF is available here.
PDF is available here.
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Abstract
Methanogenic archaea are a group of strictly anaerobic microorganisms characterized by their strict dependence on the process of methanogenesis for energy conservation. Among the archaea, they are also the only known group synthesizing proteins containing selenocysteine or pyrrolysine. All but one o...
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PMID: 20847933
PDF is available here.
PDF is available here.
Abstract
Anaerobic digestion sludge was cultivated in an electrochemical bioreactor (ECB) to enrich the hydrogenotrophic methanogens. A modified graphite felt cathode with neutral red (NR-cathode) was charged with electrochemical reducing power generated from a solar cell. The methane and carbon dioxide coll...
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PMID: 20075635
PDF is available here.
PDF is available here.
Methods in Molecular Biology (532)532 2009
Abstract
Horizontal gene transfer (HGT) is a driving force in the evolution of metabolic pathways, allowing novel enzymatic functions that provide a selective advantage to be rapidly incorporated into an organism's physiology. Here, the role of two HGT events in the evolution of methanogenesis is described....
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PMID: 19271184
PDF is available here.
PDF is available here.
Abstract
The spatial distribution, quantity and diversity of different microorganisms within anaerobic granular sludge from a lab-scale expanded granular sludge bed (EGSB) reactor operated at different organic loading rates were studied using florescent in situ hybridization (FISH), real time quantitative-po...
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PMID: 20067149
PDF is available here.
PDF is available here.
Mikrobiologiia (78)2 2009
Abstract
Mikrobiologiia (78)2 2009
Abstract
Abstract
The first articles published by Anatol Zhabotinsky have been analyzed. The mechanisms of the Belousov-Zhabotinsky and Bray-Liebhafsky oscillating chemical reactions were compared. It was shown that the traditional chemical kinetics, the new methods of molecular biology as well as isotopic compositio...
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PMID: 20067197
PDF is available here.
PDF is available here.
Abstract
We surveyed the archaeal assemblage in a stratified sulfurous lake (Lake Vilar, Banyoles, Spain) over 5 consecutive years to detect potential seasonal and interannual trends in the free-living planktonic Archaea composition. The combination of different primer pairs and nested PCR steps revealed an...
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PMID: 18754782
PDF is available here.
PDF is available here.
Archives of Microbiology (190)3 2008
Abstract
We were able to grow both species in a stable coculture. Very interestingly, M. kandleri could adhere to glass under such conditions, but not P. furiosus. This latter archaeum, however, was able to adhere onto M. kandleri cells and onto itself, resulting in structured biofilms on glass. These very o...
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PMID: 18438643
PDF is available here.
PDF is available here.
Abstract
We have developed a technique for cultivation of chemolithoautotrophs under high hydrostatic pressures that is successfully applicable to various types of deep-sea chemolithoautotrophs, including methanogens. It is based on a glass-syringe-sealing liquid medium and gas mixture used in conjunction wi...
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PMID: 18664583
PDF is available here.
PDF is available here.
Abstract
The two-phase leach-bed system is a biogas reactor system optimized for the utilization of energy crop silages at maximized loading rates under maintenance of an optimal microbial activity. In this study, a characterization of the methanogenic microbial community within this reactor system was condu...
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PMID: 18501543
PDF is available here.
PDF is available here.
Abstract
We assumed that random and independent mutations on the rRNA and lipid biosynthesis genes may result largely in coincided evolution. The fact that RNA and lipid are semantide and episemantic molecules, respectively, is the fundamental difference between the phylogeny of RNA and lipid. Moreover, diff...
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PMID: 18515030
PDF is available here.
PDF is available here.
Abstract
I (ZC-I) affiliated to Methanosarcinales, could be dominant. Using quantitative polymerase chain reaction (qPCR) assay, ZC-I methanogens were estimated to be 10(7) cells per gram of soil, accounting for about 30% of the total Archeae. By combining culturable enrichment with qPCR assay, the quantity...
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PMID: 18373675
PDF is available here.
PDF is available here.
Abstract
In this study, the biodegradation characteristics of six plant-based wastes were compared in anaerobic batch vial systems. The highest gas accumulation and methane (CH4) concentrations (approximately 70%) were observed in samples containing copy paper, newspaper and box paper materials, whereas the...
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PMID: 18649574
PDF is available here.
PDF is available here.
Geobiology (6)3 2008
Abstract
Terry Beveridge's enthusiasm about the ingenuity of microorganisms has stimulated many new avenues of microbial research. One example where Terry's observations helped direct the scientific process was in the analysis of the draft genome of the thermoacidophilic archaeum, Aciduliprofundum boonei. Th...
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PMID: 18445019
PDF is available here.
PDF is available here.
Abstract
The effect of omitting zinc from the influent of mesophilic (30 degrees C) methanol fed upflow anaerobic sludge bed (UASB) reactors, and latter zinc supplementation to the influent to counteract the deprivation, was investigated by coupling the UASB reactor performance to the microbial ecology of th...
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PMID: 18283507
PDF is available here.
PDF is available here.
Extremophiles (12)3 2008
Abstract
Two genes encoding esterases EstA and EstB of Picrophilus torridus were identified by the means of genome analysis and were subsequently cloned in Escherichia coli. PTO 0988, which is encoding EstA, consists of 579 bp, whereas PTO 1141, encoding EstB, is composed of 696 bp, corresponding to 192 aa a...
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PMID: 18330499
PDF is available here.
PDF is available here.
Abstract
Our comparative study explored the coupling of AOM with sulfate reduction (SR) and methane generation (MOG) in microbial communities from Gulf of Mexico cold seep sediments that were naturally enriched with methane and other hydrocarbons. These sediments harbour a variety of ANME clades and SRB. Fol...
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PMID: 18218032
PDF is available here.
PDF is available here.
Abstract
We evaluated the DiversiLab system from Bacterial Barcodes that utilizes a kit-based repetitive sequence-based (rep-PCR) method that has been optimized for genotyping DNA was extracted from the source organisms using either a standard chemical DNA extraction kit or Whatman FTA paper. Rep-PCR was per...
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PMID: 18302972
PDF is available here.
PDF is available here.
Journal of Bacteriology (190)10 2008
Abstract
We investigated the ubiquity and proportions of methanogenic archaea, sulfate reducers, and acetogens in plaque samples from 102 periodontitis patients. In contrast to the case for 65 healthy control subjects, hydrogenotrophic groups were almost consistently detected in periodontal pockets, with the...
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PMID: 18326571
PDF is available here.
PDF is available here.
Water Research (42)8-9 2008
Abstract
Active mesophilic and thermophilic phenol-degrading methanogenic consortia were obtained after an 18-month acclimation and enriching process in the serum bottles, and characterized using the rRNA-based molecular approach. As revealed by cloning, fluorescence in situ hybridization (FISH) and terminal...
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PMID: 18234274
PDF is available here.
PDF is available here.
Water Research (42)8-9 2008
Abstract
This paper evaluates the use of upflow anaerobic sludge bed (UASB) bioreactors (30 degrees C, pH=7.0) to remove selenium oxyanions from contaminated waters (790 microg Se L(-1)) under methanogenic and sulfate-reducing conditions using lactate as electron donor. One UASB reactor received sulfate at d...
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PMID: 18177686
PDF is available here.
PDF is available here.
Abstract
We propose the name Methanocella paludicola gen. nov., sp. nov. for the isolate, the first of the RC-I lineage. The type strain is SANAET (=JCM 13418T=NBRC 101707T=DSM 17711T). In addition, we also propose the status of order for the RC-I lineage, for which we propose the name Methanocellales ord. n...
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PMID: 18398197
PDF is available here.
PDF is available here.
Abstract
Phenol degradation under methanogenic conditions has long been studied, but the anaerobes responsible for the degradation reaction are still largely unknown. An anaerobe, designated strain UI(T), was isolated in a pure syntrophic culture. This isolate is the first tangible, obligately anaerobic, syn...
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PMID: 18281436
PDF is available here.
PDF is available here.
Current Microbiology (56)3 2008
Abstract
We evaluated the tolerance of seven methanogenic strains (Methanobrevibacter arboriphilicus, Methanobacterium formicicum, Methanococcus vannielii, Methanospirillum hungatei, Methanoculleus olentangyi, Methanoplanus limicola, and Methanosarcina mazei) to long-term exposure to air/nitrogen and drying....
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PMID: 17990030
PDF is available here.
PDF is available here.
Trends in Genetics (24)2 2008
Abstract
Our findings suggest that cysteine plays an important role in early forms of life....
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PMID: 18192060
PDF is available here.
PDF is available here.
Abstract
A simple, sensitive, and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for determining urinary creatinine was developed and used to evaluate 24 h urine samples collected during an exposure study. Urine (1 microL) was diluted with methanol and then directly applied to LC-M...
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PMID: 18092755
PDF is available here.
PDF is available here.
Journal of Molecular Biology (375)3 2008
Abstract
We have cloned, overexpressed and characterized the stator-forming subunits E and H of the A-ATPase from the thermoacidophilic Archaeon, Thermoplasma acidophilum. Size exclusion chromatography, CD, matrix-assisted laser desorption ionization time-of-flight mass spectrometry and NMR spectroscopic exp...
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PMID: 18036615
PDF is available here.
PDF is available here.
Abstract
The priority results obtained during 35 years, when studying biology of methane-oxidizing bacteria, are presented. There is more profound representation of the results obtained for the recent 5 years of the Department activity: phylogenetic classification of methylotrophic bacteria, biodiversity of...
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PMID: 18663925
PDF is available here.
PDF is available here.
Abstract
These results indicate that haloarchaeon Sech7a excretes a novel bacteriocin....
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PMID: 18569870
PDF is available here.
PDF is available here.
Abstract
Methane-forming archaea are strictly anaerobic, ancient microbes that are widespread in nature. These organisms are commonly found in anaerobic environments such as rumen, anaerobic sediments of rivers and lakes, hyperthermal deep sea vents and even hypersaline environments. From an evolutionary sta...
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PMID: 17713742
PDF is available here.
PDF is available here.
Abstract
We found a decreasing relative abundance of marine Crenarchaeota Group I throughout the meso- and bathypelagic water column from 65 degrees N to 5 degrees N in the eastern basin of the North Atlantic. Thus, the relative contribution of marine Crenarchaeota Group I to deep-water prokaryotic communiti...
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PMID: 18211271
PDF is available here.
PDF is available here.
Abstract
Membrane-inserted complexes consisting of two photochemically reactive sensory rhodopsin (SR) subunits flanking a homodimer of a transducing protein subunit (Htr) are used by halophilic archaea for sensing light gradients to modulate their swimming behavior (phototaxis). The SR-Htr complexes extend...
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PMID: 18346091
PDF is available here.
PDF is available here.
Abstract
The microbial community structures in the thermophilic anaerobic digestion (TAD) of waste activated sludge (WAS) and WAS were analyzed with molecular biological techniques including real-time polymerase chain reaction (PCR) and cloning. The microbial community of TAD had less diversity than that of...
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PMID: 18469390
PDF is available here.
PDF is available here.
Abstract
We propose PeiW treatment as a novel permeabilization method for CARD-FISH application to methanogens....
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PMID: 18022717
PDF is available here.
PDF is available here.
Abstract
Methyl coenzyme-M reductase A (mcrA) clone libraries were generated from microbial DNA extracted from the rumen of cattle fed a roughage diet with and without supplementation of the antimethanogenic compound bromochloromethane. Bromochloromethane reduced total methane emissions by c. 30%, with a res...
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PMID: 17949432
PDF is available here.
PDF is available here.
Abstract
The spatial and temporal distribution of pelagic Archaea was studied in the southern North Sea by rRNA hybridization, sequencing and quantification of 16S rRNA gene and membrane lipid analyses and related to physical, chemical and biological parameters to determine the factors influencing archaeal b...
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PMID: 17991018
PDF is available here.
PDF is available here.
Microbial Ecology (54)4 2007
Abstract
The phylogenetic diversity of the bacterial and archaeal community in the water and sediments of three large lakes of the Wadi An Natrun was investigated using 16S rRNA clone libraries. The bacterial community was diverse: 769 clones formed 345 operational taxonomic units (OTUs) defined at 99% 16S r...
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PMID: 17450395
PDF is available here.
PDF is available here.
Microbial Ecology (54)3 2007
Abstract
Archaeal 16S rRNA gene sequences have been found in a variety of moderate-temperature habitats including soil and rhizospheres. In this study, the differences of archaeal communities associated with Scots pine (Pinus sylvestris L.) short roots, different types of mycorrhizospheric compartments, and...
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PMID: 17334967
PDF is available here.
PDF is available here.
Abstract
We used real-time single-molecule micromechanical experiments to show that topoisomerase V relaxes DNA via events that release multiple DNA turns, employing a constrained swiveling mechanism similar to that for type IB enzymes. Relaxation is powered by the torque in the supercoiled DNA and is constr...
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PMID: 17804808
PDF is available here.
PDF is available here.
Abstract
We examine isolates of the haloarchaeal genus Halorubrum from two adjacent ponds of different salinities at a Spanish saltern and a natural saline lake in Algeria by using multilocus sequence analysis. We show that, although clusters can be defined by concatenation of multiple marker sequences, barr...
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PMID: 17715057
PDF is available here.
PDF is available here.
Abstract
The effect of sulfide on nitrate reduction and methanogenesis was investigated in two mixed, mesophilic (35 degrees C) methanogenic cultures: sulfide-free and sulfide-acclimated (67 mg S/L total sulfide). A mixture of dextrin/peptone served as the carbon/electron donor source for the two stock cultu...
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PMID: 17238206
PDF is available here.
PDF is available here.
Journal of Bacteriology (189)15 2007
Abstract
Archaea are abundant and drive critical microbial processes in the Earth's cold biosphere. Despite this, not enough is known about the molecular mechanisms of cold adaptation and no biochemical studies have been performed on stenopsychrophilic archaea (e.g., Methanogenium frigidum). This study exami...
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PMID: 17545280
PDF is available here.
PDF is available here.
Abstract
Abstract
An extremely acidic (pH 2.5-2.75) metal-rich stream draining an abandoned mine in the Iberian Pyrite Belt, Spain, was ramified with stratified macroscopic gelatinous microbial growths ('acid streamers' or 'mats'). Microbial communities of streamer/mat growths sampled at different depths, as well as...
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PMID: 17564609
PDF is available here.
PDF is available here.
Abstract
We compared the prokaryotic communities of three sites located in the LHF and encountered a predominance of archaeal sequences affiliated with methanogenic Methanococcales at all three. However, the bacterial composition varied in accordance with differences in fluid chemistry between the three site...
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PMID: 17506828
PDF is available here.
PDF is available here.
Abstract
This study summarizes the findings of our research on the genesis of methane, its content and distribution in permafrost horizons of different age and origin. Supported by reliable data from a broad geographical sweep, these findings confirm the presence of methane in permanently frozen fine-grained...
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PMID: 17428301
PDF is available here.
PDF is available here.
Abstract
We examined the survival potential of methanogenic archaea exposed to different environmental stress conditions such as low temperature (down to -78.5 degrees C), high salinity (up to 6 M NaCl), starvation (up to 3 months), long-term freezing (up to 2 years), desiccation (up to 25 days) and oxygen e...
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PMID: 17428303
PDF is available here.
PDF is available here.
Abstract
The molecular diversity of rumen methanogens in feedlot cattle and the composition of the methanogen populations in these animals from two geographic locations were investigated using 16S rRNA gene libraries prepared from pooled PCR products from 10 animals in Ontario (127 clones) and 10 animals fro...
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PMID: 17483285
PDF is available here.
PDF is available here.
Abstract
DNA-based microbial source tracking (MST) methods were developed and used to specifically and sensitively track the unintended aerosolization of land-applied, anaerobically digested sewage sludge (biosolids) during high-wind events. Culture and phylogenetic analyses of bulk biosolids provided a basi...
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PMID: 17513591
PDF is available here.
PDF is available here.
Bioresource Technology (98)8 2007
Abstract
This paper discusses the methanogenic digestion of a synthetic acetic acid wastewater inoculated using a mixed culture obtained from an anaerobic digester at a municipal wastewater treatment facility. Experiments were conducted in 500mL batch reactors containing an unbuffered acetic acid solution. T...
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PMID: 16996264
PDF is available here.
PDF is available here.
Abstract
The anaerobic treatment of saline effluents using halophilic and halotolerant microbial consortia is of major interest. Inhibition of anaerobic digestion is known to occur at high salt content. However, it seems that the suitable adaptation of an anaerobic sludge makes possible the treatment of sali...
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PMID: 17245575
PDF is available here.
PDF is available here.
Abstract
We constructed two mesophilic anaerobic chemostats that were continuously fed with synthetic wastewater containing butyrate as the sole source of carbon and energy. Steady-state conditions were achieved at dilution rates between 0.025 and 0.7 day(-1). Butyrate, fed into the chemostat, was almost com...
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PMID: 17221191
PDF is available here.
PDF is available here.
Abstract
I and II were tests with a two-stage continuous-batch process in a thermophilic battery of six digesters and a designated post-digestion train that was isolated from mesophilic operations. These tests demonstrated that digester outflow biosolids met the Class A limits for fecal coliforms and Salmone...
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PMID: 17054113
PDF is available here.
PDF is available here.
Abstract
The microbial community and its diversity in production water from a high-temperature, water-flooded petroleum reservoir of an offshore oilfield in China were characterized by 16S rRNA gene sequence analysis. The bacterial and archaeal 16S rRNA gene clone libraries were constructed from the communit...
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PMID: 17286581
PDF is available here.
PDF is available here.
Abstract
These results support the conclusion that the H-bonding interaction at the N(5) plays a key role in establishing the high Eox/sq and the unusually high stability of the anionic semiquinone state in wETF....
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PMID: 17291008
PDF is available here.
PDF is available here.
Abstract
The diversity of Archaea was studied in vertisolic and loam soils of a semi-arid region in Australia. Sampling was undertaken at an agricultural site, two grassland environments, and a brigalow (Acacia harpophylla) woodland. Archaeal community structure was profiled using amplified ribosomal DNA res...
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PMID: 17496958
PDF is available here.
PDF is available here.
Abstract
Water Research (41)1 2007
Abstract
A 6-L, completely mixed anaerobic bioreactor with an external ultrafiltration membrane module was operated for 300 days to evaluate the startup and performance of an anaerobic membrane bioreactor (AnMBR) treating swine manure. The reactor had a successful startup at the initial loading rate of 1g vo...
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PMID: 17109913
PDF is available here.
PDF is available here.