Abstract
We isolated a protein complex, by using detergent-induced solubilization of cell envelopes, followed by a combination of chromatography steps. Analysis by MS and comparison with databases revealed that this fraction contained two dominant proteins, representing the respective major envelope proteins...
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PMID: 19143616
PDF is available here.
Abstract
The complete genome sequence of the crenarchaeon Ignicoccus hospitalis published recently in Genome Biology provides a great leap forward in the dissection of its unique association with another hyperthermophilic archaeon, Nanoarchaeum equitans.
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PMID: 19216728
PDF is available here.
Abstract
The introns early hypothesis predicts that introns were fundamental in assembling the first genes. In Nanoarchaeum equitans some genes are split into two. If these split genes were the ancestral forms, as suggested by the introns early hypothesis, then the end-beginning of the two parts of the split...
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PMID: 18590807
PDF is available here.
Abstract
Ultrastructure and intercellular interaction of Ignicoccus hospitalis and Nanoarchaeum equitans were investigated using two different electron microscopy approaches, by three-dimensional reconstructions from serial sections, and by electron cryotomography. Serial sections were assembled into 3D reco...
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PMID: 18622597
PDF is available here.
Abstract
We have designed a new set of universal archaeal primers that amplify the 16S rRNA gene of all four archaeal sub-divisions, and present two new sets of Nanoarchaeota-specific primers based on all known nanoarchaeal 16S rRNA gene sequences. These primers can be used to detect N. equitans and have gen...
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PMID: 18553053
PDF is available here.
Abstract
We show that the conserved placement of its tRNA gene promoters allows the synthesis of leaderless tRNAs, whose presence was verified by the observation of 5' triphosphorylated mature tRNA species. Initiation of tRNA gene transcription requires a purine, which coincides with the finding that tRNAs w...
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PMID: 18451863
PDF is available here.
Abstract
Superoxide reductases (SORs) are antioxidant enzymes present in many prokaryotes, either anaerobes or microaerophiles, which detoxify superoxide by reducing it to hydrogen peroxide. The reaction mechanism involves the diffusion-limited encounter of superoxide with the reduced iron site and concomita...
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PMID: 17968598
PDF is available here.
Abstract
We investigate the phylogenetic position of Nanoarchaeum equitans. The results obtained are compatible with the hypothesis that N. equitans represents a new phylum within the Archaea domain because the characteristic long branch of N. equitans in phylogenetic trees is conserved even after most of th...
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PMID: 18508702
PDF is available here.
Abstract
The Pyrococcus abyssi genome displays two genes possibly coding for S-adenosyl-l-methionine-dependent RNA(uracil, C5)-methyltransferases (PAB0719 and PAB0760). Their amino acid sequences are more closely related to Escherichia coli RumA catalysing the formation of 5-methyluridine (m(5)U)-1939 in 23S...
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PMID: 18069966
PDF is available here.
Mircea Podar,
Iain Anderson,
Kira S Makarova,
James G Elkins,
Natalia Ivanova,
Mark A Wall,
Athanasios Lykidis,
Kostantinos Mavromatis,
Hui Sun,
Matthew E Hudson,
Wenqiong Chen,
Cosmin Deciu,
Don Hutchison,
Jonathan R Eads,
Abraham Anderson,
Fillipe Fernandes,
Ernest Szeto,
Alla Lapidus,
Nikos C Kyrpides,
Milton H Saier,
Paul M Richardson,
Reinhard Rachel,
Harald Huber,
Jonathan A Eisen,
Eugene V Koonin,
Martin Keller and
Karl O Stetter
Abstract
A combination of genomic and cellular features suggests highly efficient adaptation to the low energy yield of sulfur-hydrogen respiration and efficient inorganic carbon and nitrogen assimilation. Evidence of lateral gene exchange between N. equitans and I. hospitalis indicates that the relationship...
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PMID: 19000309
PDF is available here.
Abstract
We name Ignicoccus hospitalis sp. nov. (type strain KIN4/I(T)=DSM 18386(T)=JCM 14125(T))....
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PMID: 17392210
PDF is available here.
Abstract
A model has been proposed suggesting that the tRNA molecule must have originated by direct duplication of an RNA hairpin structure [Di Giulio, M., 1992. On the origin of the transfer RNA molecule. J. Theor. Biol. 159, 199-214]. A non-monophyletic origin of this molecule has also been theorized [Di G...
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PMID: 16289209
PDF is available here.
Abstract
Our results are the first evidence to show experimentally that natural protein trans-splicing occurs in an archaeal protein, a thermostable protein, and a family B-type DNA polymerase....
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PMID: 16412462
PDF is available here.
Abstract
We therefore developed SPLITS, which is aimed at searching for any type of tRNA gene and is especially focused on intron-containing tRNAs or split tRNAs at the genome level. SPLITS initially predicts the bulge-helix-bulge splicing motif (a well-known, required structure in archaeal pre-tRNA introns)...
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PMID: 17274770
PDF is available here.
Abstract
We removed actively venting chimneys and in their place deployed mineral chambers and sampling units that promoted the growth of new, natural hydrothermal chimneys and allowed their collection within hours of formation. These samples were compared with those collected from established hydrothermal c...
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PMID: 16343327
PDF is available here.
Abstract
Analysis of nucleotide, codon and amino acid usage patterns in N. equitans indicates the presence of distinct selective constraints, probably due to its adaptation to a thermo-parasitic life-style. Among the conspicuous characteristics featuring its hyperthermophilic adaptation are overrepresentatio...
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PMID: 16869956
PDF is available here.
Abstract
We show that the N. equitans splicing endonuclease cleaves tRNA precursors containing normal introns, as well as all five noncontinuous precursor tRNAs, at the predicted splice sites, indicating the enzyme's dual role in the removal of tRNA introns and processing of tRNA halves to be joined in trans...
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PMID: 16330750
PDF is available here.
Abstract
In the past two years, archaeal genomics has achieved several breakthroughs. On the evolutionary front the most exciting development was the sequencing and analysis of the genome of Nanoarchaeum equitans, a tiny parasitic organism that has only approximately 540 genes. The genome of Nanoarchaeum sho...
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PMID: 16111915
PDF is available here.
Abstract
We show that the intron-containing tRNA(Trp) was misidentified in the initial Nanoarchaeum equitans genome annotation [E. Waters et al. (2003) The genome of Nanoarchaeum equitans: insights into early archaeal evolution and derived parasitism. Proc. Natl. Acad. Sci. USA 100, 12984-12988]. Along with...
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PMID: 15893316
PDF is available here.
Abstract
We develop a computational approach to genome analysis that searches for widely separated genes encoding tRNA halves that, on the basis of structural prediction, could form intact tRNA molecules. A search of the N. equitans genome reveals nine genes that encode tRNA halves; together they account for...
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PMID: 15690044
PDF is available here.
Abstract
We present phylogenetic analyses of prolyl-tRNA and alanyl-tRNA synthetase genes that indicate lateral gene transfer events to an ancestor of the diplomonads and parabasalids from lineages more closely related to the newly discovered archaeal hyperthermophile Nanoarchaeum equitans (Nanoarchaeota) th...
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PMID: 15356278
PDF is available here.
Abstract
Nanobacteria are suspected to be responsible for a number of diseases, i.e., kidney stones, heart disease, ovarian cancer, peripheral neuropathy, and reduced bone mineral density. Being protected by a mineral shell consisting of apatite, the nanovesicles can enter eukaryotic cells. Depending on the...
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PMID: 15952752
PDF is available here.
Abstract
We tested the placement of N. equitans in the archaeal phylogeny using a large dataset of concatenated ribosomal proteins from 25 archaeal genomes. We indicate that the placement of N. equitans in archaeal phylogenies on the basis of ribosomal protein concatenation may be strongly biased by the coup...
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PMID: 15892870
PDF is available here.