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Enteropathogenic Escherichia coli (0):
Recent article
Enteropathogenic Escherichia coli
Infection and Immunity (78)8 2010
Abstract
We examine the organization of clathrin in pedestals and report that CD2AP, an endocytosis-associated and cortactin-binding protein, is a novel and important component of EPEC pedestal formation that also utilizes Y474 phosphorylation of EPEC Tir. We also demonstrate the successive recruitment of Nc...
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PMID: 20515931
PDF is available here.
PDF is available here.
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Infection and Immunity (78)7 2010
Abstract
We have previously observed type 1 pilus-mediated interleukin-8 (IL-8) hyperproduction in infected neutrophils. As pathogen induction of host cell death programs and clearance of apoptotic infected cells are crucial for innate immune system homeostasis and host integrity, we examined modulation of n...
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PMID: 20404079
PDF is available here.
PDF is available here.
Journal of Bacteriology (192)13 2010
Abstract
We report that the N terminus of the type III secretion system translocator proteins EspB, EspD, and EspA mediate protein secretion and translocation from wild-type enteropathogenic Escherichia coli and hypersecretion from sepL and sepD mutants. EspA containing the translocation signal of Map and Ti...
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PMID: 20400543
PDF is available here.
PDF is available here.
Molecular Microbiology (76)5 2010
Abstract
We inactivated the Cpx response in enteropathogenic Escherichia coli (EPEC), a causative agent of infant diarrhoea, and observed decreased expression of its major adhesin, the bundle-forming pilus (BFP). Here, we examined the mechanism underlying this BFP expression defect, and found that this pheno...
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PMID: 20444097
PDF is available here.
PDF is available here.
Microbiology (156)Pt 6 2010
Abstract
We have shown that the enteropathogenic Escherichia coli (EPEC) type III secretion system effector NleH has a broad-range anti-apoptotic activity. In this study we examined the effects of NleH on cells challenged with TcdB. During infection with wild-type EPEC, NleH inhibited TcdB-induced apoptosis...
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PMID: 20223805
PDF is available here.
PDF is available here.
Abstract
We found that among 139 E. coli strains analyzed, 20 (14.4%) corresponded to E. coli diarrheagenic pathotypes. Enterotoxigenic, shiga-toxin-producing, enteroaggregative, diffuse adherent, and enteropathogenic E. coli pathotypes were detected, and most of them belonged to the phylogenetic groups A an...
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PMID: 19839760
PDF is available here.
PDF is available here.
Abstract
Escherichia coli was isolated from the feces of 122 piglets with diarrhea on 55 farms in Korea. The virulence genes of each isolate were characterized by polymerase chain reaction (PCR). Of the 562 isolates, 191 carried 1 or more of the virulence genes tested for in this study. Of the 191 isolates,...
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PMID: 20357961
PDF is available here.
PDF is available here.
Abstract
Although atypical enteropathogenic Escherichia coli (aEPEC) strains are frequently implicated in childhood diarrhea in developing countries, not much is known about their adherence properties. The phenotypic and genotypic characterization of 29 aEPEC strains expressing the localized adherence-like p...
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PMID: 19864474
PDF is available here.
PDF is available here.
PLoS Pathogens (6)1 2010
Abstract
We show that inhibition of NF-kappaB activation by EPEC involves the injection of NleE into the host cell. Importantly, we show that NleE inhibits NF-kappaB activation by preventing activation of IKKbeta and consequently the degradation of the NF-kappaB inhibitor, IkappaB. This NleE activity is enha...
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PMID: 20126447
PDF is available here.
PDF is available here.
Abstract
A collection of 69 eae-positive strains expressing 29 different intimin types and eight tir alleles was characterized with respect to their adherence patterns to HeLa cells, ability to promote actin accumulation in vitro, the presence of bfpA alleles in positive strains, and bundle-forming pilus (BF...
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PMID: 20112229
PDF is available here.
PDF is available here.
Abstract
We were unable to observe any significant growth advantage in different E. coli strains transformed with p6148. The p6148 DNA sequence is homologous in sequence and arrangement to DNA from other plasmid families, including large conjugative plasmids and SXT integrative and conjugative elements. This...
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PMID: 19940931
PDF is available here.
PDF is available here.
Abstract
We report that GAPDH of these pathogens is ADP-ribosylated either in the cytoplasm or in the extracellular medium. GAPDH catalyzes its own modification, which involves Cys-149 at the active site. ADP-ribosylation of extracellular GAPDH may play an important role in the host-pathogen interaction, as...
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PMID: 19784925
PDF is available here.
PDF is available here.
Journal of Medical Microbiology (58)Pt 8 2009
Abstract
We report the results of a large screen aimed at determining the prevalence of espM and espT among clinical EPEC and EHEC isolates. The results suggest that espM, detected in 51 % of the tested strains, is more commonly found in EPEC and EHEC serogroups that are linked to severe human infections. In...
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PMID: 19528152
PDF is available here.
PDF is available here.
Experimental Cell Research (315)12 2009
Abstract
We show that Shank3 is present in both EPEC- and S. typhimurium-induced actin rearrangements and is required for optimal EPEC pedestal formation. We propose that Shank3 is one of a number of host synaptic proteins likely to play key roles in bacteria-host interactions....
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PMID: 19371741
PDF is available here.
PDF is available here.
Abstract
Overall, during the period of April 2003 to May 2004, a total of 1399 food handlers stool samples were collected and analysed. EPEC expressing the eaeA gene and STEC expressing the stx2 gene were detected in 11/1399 (0.8%) and 2/1399 (0.1%) of the study subjects respectively. The mean age of the sub...
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PMID: 20358790
PDF is available here.
PDF is available here.
Abstract
Enteropathogenic Escherichia coli (EPEC) infections are a leading cause of infantile diarrhea in developing nations. Multilocus sequence typing (MLST) characterizes bacterial strains based on the sequences of internal fragments in housekeeping genes. Little is known about strains of EPEC analyzed by...
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PMID: 19767837
PDF is available here.
PDF is available here.
Abstract
Cytolethal distending toxins (CDTs) represent an emerging family of newly described bacterial products that are produced by a number of pathogens. The genes encoding these toxins have been identified as a cluster of three adjacent genes, cdtA, cdtB, and cdtC, plus 5 cdt genetic variants, designated...
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PMID: 19494702
PDF is available here.
PDF is available here.
Cell Host & Microbe (5)5 2009
Abstract
We hypothesized that exposure to pathogens might not only cause worms to move away but also simultaneously activate pathways that promote resistance to the pathogen. We show that brief exposure to virulent or avirulent strains of the bacterial pathogen enteropathogenic E. coli (EPEC) "immunizes"C. e...
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PMID: 19454349
PDF is available here.
PDF is available here.
Cellular Microbiology (11)4 2009
Abstract
We have identified and characterized a novel TTSS-dependent effector protein encoded at the 5'-end of the locus of enterocyte effacement (LEE) pathogenicity island (PAI) of ATEC strain 3431-4/86 (O8:H(-)). Using affinity purification we identified IQGAP1, a scaffolding protein involved in the regula...
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PMID: 19134119
PDF is available here.
PDF is available here.
Infection and Immunity (77)4 2009
Abstract
We constructed C. rodentium strains expressing TccP. Using a mouse intestinal in vitro organ culture model and oral gavage into C57BL/6 mice, we have shown that TccP can cooperate with Tir of C. rodentium. The recombinant strains induced typical A/E lesions ex vivo and in vivo. Expression of TccP di...
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PMID: 19188355
PDF is available here.
PDF is available here.
FEMS Microbiology Letters (292)2 2009
Abstract
The 157-kb conjugative plasmid pEO5 encoding alpha-haemolysin in strains of human enteropathogenic Escherichia coli (EPEC) O26 was investigated for its relationship with EHEC-haemolysin-encoding plasmids of enterohaemorrhagic E. coli (EHEC) O26 and O157 strains. Plasmid pEO5 was found to be compatib...
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PMID: 19175679
PDF is available here.
PDF is available here.
Cellular Microbiology (11)3 2009
Abstract
In vitro organ culture (IVOC) represents a gold standard model to study enteropathogenic E. coli (EPEC) infection of human intestinal mucosa. However, the optimal examination of the bacterial-host cell interaction requires a directional epithelial exposure, without serosal or cut surface stimulation...
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PMID: 19134113
PDF is available here.
PDF is available here.
Cellular Microbiology (11)3 2009
Abstract
We show that clustering transfers Tir into cholesterol-rich detergent-resistant microdomains (DRMs), a signal prompting transient c-Fyn accumulation at bacterial adhesion sites. Co-clustering of Tir(Y474) and c-Fyn in DRMs rapidly stimulates robust kinase activation both by induced c-Fyn(Y531) depho...
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PMID: 19016778
PDF is available here.
PDF is available here.
Abstract
In order to obtain an attenuated vaccine candidate for enteropathogenic Escherichia coli (EPEC) O45, a ler deletion mutant of pig enteropathogenic E. coli (PEPEC) O45 was constructed by using the suicide vector pCVD442, termed as PEPEC O45(deltaler). The culture supernatant of PEPEC O45(deltaler) de...
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PMID: 19459321
PDF is available here.
PDF is available here.
Cellular Microbiology (11)2 2009
Abstract
We show that Map triggers filopodia formation by activating Cdc42; expression of dominant-negative Cdc42 or knock-down of Cdc42 by siRNA impaired filopodia formation. In addition, Map binds PDZ1 of NHERF1. We show that Map-NHERF1 interaction is needed for filopodia stabilization in a process involvi...
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PMID: 19046338
PDF is available here.
PDF is available here.
Eastern Mediterranean health journal = La revue de sante de la Mediterranee orientale = al-Majallah al-sihhiyah li-sharq al-mutawassit (15)5 2009
Abstract
A cross-sectional hospital-based study was carried out at Tikrit teaching hospital, Iraq, from October 2004 to September 2005, to identify the prevalence and etiology of nosocomial infectious diarrhoea among children under 5 years of age. Of 259 children admitted to the paediatric ward for reasons o...
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PMID: 20214124
PDF is available here.
PDF is available here.
Abstract
We demonstrate that enhanced lysozyme resistance of enteropathogenic Escherichia coli requires the plasmid-encoded regulator, Per, and is mediated by factors outside the locus for enterocyte effacement. EspC, a Per-activated serine protease autotransporter protein, conferred enhanced resistance on n...
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PMID: 18997020
PDF is available here.
PDF is available here.
Abstract
We have developed a polymerase chain reaction (PCR)-based assay for use in areas with limited resources to screen for diarrheogenic strains from clinical isolates. To simplify the assay and minimize reagents, our method implemented the use of plasmids rather than bacteria as template controls and th...
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PMID: 18990527
PDF is available here.
PDF is available here.
Journal of Bacteriology (191)1 2009
Abstract
We determined the complete genomic sequence of E2348/69 and performed genomic comparisons with other important E. coli strains. We identified 424 E2348/69-specific genes, most of which are carried on mobile genetic elements, and a number of genetic traits specifically conserved in phylogroup B2 stra...
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PMID: 18952797
PDF is available here.
PDF is available here.
BMC Microbiology (9)1 2009
Abstract
We observed that flagellin was consistently and strongly present in the secretome of wild type EPEC and the DeltaespADB mutant but present only weakly in the secretome of the DeltaescF mutant. Given the ancestral relationship between the flagella export apparatus and virulence associated T3SSs, we i...
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PMID: 19200386
PDF is available here.
PDF is available here.
BMC Microbiology (9) 2009
Abstract
Five of six strains invaded HeLa and T84 cells in a range of 13.3%-20.9% and 5.8%-17.8%, respectively, of the total cell-associated bacteria. The strains studied were significantly more invasive than prototype tEPEC strain E2348/69 (1.4% and 0.5% in HeLa and T84 cells, respectively). Invasiveness wa...
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PMID: 19622141
PDF is available here.
PDF is available here.
Abstract
Nineteen studies were included. Some used episodes of diarrhoea as the unit of analysis, others used children. The quality of reporting of laboratory procedures varied, and pathogens (particularly E. coli types) were classified in different ways. As there were no apparent regional differences in pat...
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PMID: 19515227
PDF is available here.
PDF is available here.
BMC Microbiology (9) 2009
Abstract
The results showed that aEPEC are highly heterogeneous. Multilocus sequence typing revealed that 61 of 75 aEPEC strains did not belong to known tEPEC or STEC clades, and of those that did, none expressed an O:H serotype that is frequent in tEPEC or STEC strains associated with disease. PCR for each...
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PMID: 19490652
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (283)50 2008
Abstract
We report that IQGAP1, Ca(2+), and calmodulin modulate actin pedestal formation by EPEC. Infection with EPEC promotes both the interaction of IQGAP1 with calmodulin and the localization of IQGAP1 and calmodulin to actin pedestals while reducing the interaction of IQGAP1 with Rac1 and Cdc42. IQGAP1-n...
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PMID: 18809683
PDF is available here.
PDF is available here.
Abstract
The mechanisms mediating the association between Escherichia coli and specific hosts are unknown. This study investigates the hypothesis that the host-specific associations of E. coli strains are mediated in part by differences in chemotaxis. To test this hypothesis, chemotactic responses of E. coli...
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PMID: 19096459
PDF is available here.
PDF is available here.
Journal of Bacteriology (190)23 2008
Abstract
We screened for Ler mutants defective in autorepression. We isolated 18 different point mutations in Ler, rendering it defective in autorepression and in DNA binding. Among these mutants were those defective in positive regulation as well as in autorepression, dominant-negative mutants, and a mutant...
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PMID: 18835988
PDF is available here.
PDF is available here.
FEBS Journal (275)23 2008
Abstract
We determined that EPEC invades host cells through tight junctions as it initiates infection. Immunofluorescence microscopy revealed redistribution of the tight-junction proteins occludin and ZO-1 from an intercellular to a cytoplasmic location after EPEC invasion. Flotillin-1 was recruited to sites...
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PMID: 19016848
PDF is available here.
PDF is available here.
Microbiology (154)Pt 12 2008
Abstract
We demonstrate that mutated Ler proteins either containing H-NS alpha-helices 1 and 2, missing from Ler, or truncated for the 11 aa C-terminal extension compared with the related H-NS protein, did not appreciably alter Ler function. In contrast, mutating the proline at position 92 of Ler, in the con...
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PMID: 19047730
PDF is available here.
PDF is available here.
Biochemistry (Washington) (47)44 2008
Abstract
Escherichia coli O86 possesses high human blood group B activity because of its O-antigen structure, sharing the human blood group B epitope. In this study, the wbwK gene of E. coli O86:B7 was expressed and purified as the GST fusion protein. Thereafter, the wbwK gene was biochemically identified to...
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PMID: 18842005
PDF is available here.
PDF is available here.
Abstract
We have constructed Lactobacillus casei strains expressing different fragments of intimin beta, a subtype that is frequently expressed by EPEC strains. Mucosal immunization of mice with L. casei expressing intimin fragments induced specific systemic and mucosal antibodies. These antibodies were able...
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PMID: 18801043
PDF is available here.
PDF is available here.
Journal of Bacteriology (190)21 2008
Abstract
We performed a systemic and genome-wide survey of the GEIs in strain B171-8, one of the prototype strains of EPEC, by the combined use of whole-genome PCR scanning and fosmid mapping and identified 22 large GEIs, including nine lambda-like prophages, three P2-like prophages, the LEE, and three addit...
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PMID: 18757547
PDF is available here.
PDF is available here.
Abstract
The variability of the tir, espA, and espD genes of the locus of enterocyte effacement (LEE) in 185 attaching and effacing Escherichia coli (AEEC) strains isolated from healthy and diarrheic cattle, sheep, and goats was investigated by polymerase chain reaction. Nineteen of the strains were enterohe...
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PMID: 19086378
PDF is available here.
PDF is available here.
Cellular Microbiology (10)10 2008
Abstract
We show that during EPEC infection, EspC is secreted from the bacteria by the type V secretion system (T5SS) and then it is efficiently translocated into the epithelial cells through the type III secretion system (T3SS) translocon. By dissecting this mechanism, we found that EspC internalization dur...
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PMID: 18547338
PDF is available here.
PDF is available here.
Abstract
How invasive bacteria exploit mammalian host cell components to induce their entry into cells has received a lot of attention in the last two decades. Model organisms have emerged and helped understanding the various mechanisms that are used. Among those, Listeria monocytogenes is one of the most do...
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PMID: 18755008
PDF is available here.
PDF is available here.
Infection and Immunity (76)9 2008
Abstract
We found that EspF from rabbit enteropathogenic Escherichia coli (E22) participates in the regulation of actin polymerization by binding to a complex of proteins at the tight junctions (TJ). EspF bound to actin and profilin throughout the course of infection. However, after 2 h of infection, EspF al...
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PMID: 18559425
PDF is available here.
PDF is available here.
Cellular Microbiology (10)8 2008
Abstract
Enteropathogenic Escherichia coli (EPEC) have been previously shown to alter sodium hydrogen exchanger 3 (NHE3) activity in human intestinal epithelial cells. To further characterize these observations, PS120 fibroblasts transfected with NHE3 were studied. EPEC E2348/69 infection decreased NHE3 acti...
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PMID: 18433466
PDF is available here.
PDF is available here.
Abstract
Findings indicative of UC were seen in the combined treatment (IA + B) as well as the IA continued treatment groups: the animals showed slow rate of increase in body weight, diarrhea, bloody stools, high colonic ulcer score, as well as histological alterations characteristic of UC, with an extensive...
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PMID: 18609687
PDF is available here.
PDF is available here.
Abstract
Atypical enteropathogenetic Escherichia coli (EPEC) strains are frequently detected in children with diarrhea but are also a common finding in healthy children. The aim of this study was to compare the phylogenetic ancestry and virulence characteristics of atypical (eae positive, stx and bfpA negati...
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PMID: 18463209
PDF is available here.
PDF is available here.
Journal of Bacteriology (190)14 2008
Abstract
We previously showed that EPEC O127 forms a group 4 capsule (G4C), and in this report we show that EHEC O157 also produces a G4C, whose assembly is dependent on the etp, etk, and wzy genes. We further show that at early time points postinfection, these G4Cs appear to mask surface structures includin...
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PMID: 18502854
PDF is available here.
PDF is available here.
Microbiology (154)Pt 7 2008
Abstract
Enteropathogenic Escherichia coli (EPEC) adheres in vivo and in vitro to epithelial cells. Two main adhesins, the bundle-forming pilus and intimin, encoded by the bfp operon and eae, respectively, are responsible for the localized and the intimate adherence phenotypes. Deletion of the pst operon of...
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PMID: 18599831
PDF is available here.
PDF is available here.
Abstract
We show that a surface type II beta-turn in the Escherichia coli protein EscU undergoes auto-cleavage by a mechanism involving cyclization of a strictly conserved asparagine residue. Structural and in vivo analysis of point and deletion mutations illustrates the subtle conformational effects of auto...
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PMID: 18451864
PDF is available here.
PDF is available here.
Abstract
Enteropathogenic Escherichia coli (EPEC) is a diarrheagenic pathogen that perturbs intestinal epithelial function. Many of the alterations in the host cells are mediated by effector molecules that are secreted directly into epithelial cells by the EPEC type III secretion system. The secreted effecto...
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PMID: 18356531
PDF is available here.
PDF is available here.
Abstract
We determined the numbers of total coliforms and E. coli and examined small intestinal histology for all pigs. Complete blood counts were also determined pre- and postchallenge. Challenged pigs receiving hLZ milk had fewer total coliforms (P = 0.029) and E. coli (P = 0.030) in the ileum than control...
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PMID: 18424602
PDF is available here.
PDF is available here.
Journal of Molecular Biology (378)1 2008
Abstract
We have inserted the D3 domain of FliCi (from Salmonella typhimurium) into the outer domain of EspA and have studied the structure and function of modified filaments when expressed in an enteropathogenic E. coli espA mutant. We found that the chimeric protein EspA-FliCi filaments were biologically a...
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PMID: 18353364
PDF is available here.
PDF is available here.
Microbial Pathogenesis (44)4 2008
Abstract
We report that EPEC-induced apoptosis in T84 intestinal epithelial cells via a mechanism involving caspases-3, -6, -8, and -9, the cleavage of PARP, and oligonucleosome formation. In addition, EPEC time-dependently increased paracellular permeability as assessed by transepithelial resistance and the...
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PMID: 18054197
PDF is available here.
PDF is available here.
Abstract
Our aim was to verify if IgY obtained from chickens immunized with EPEC O111, STEC O111 and STEC O157 is able to show in vitro reactivity and biological activity towards the three bacteria. IgY was obtained from eggs laid before and after immunization with each bacterium. The preparations of IgY ant...
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PMID: 18071921
PDF is available here.
PDF is available here.
Cellular Microbiology (10)3 2008
Abstract
Enteropathogenic and enterohaemorrhagic Escherichia coli (EPEC and EHEC) colonize the gut mucosa via attaching and effacing (A/E) lesions. For years cultured cells were used as model systems to study A/E lesion formation, which showed actin accumulation under attached bacteria that can be raised abo...
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PMID: 18053003
PDF is available here.
PDF is available here.
Abstract
I Fimbriae. Based on the original sequences of Type I Fimbriae operon gene clusters in the GenBank,we generated PCR products by using primers with the homologies extension of fimH gene to be deleted and template plasmid pKD3 carrying selectable antibiotic chloramphenicol resistance (cat) gene that i...
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PMID: 18438011
PDF is available here.
PDF is available here.
Microbial Pathogenesis (44)2 2008
Abstract
We investigated the role of DsbA in enteropathogenic Escherichia coli. Here, we show that the DsbA is required for stable expression of outer membrane secretin EscC. DsbA has no effect on LEE transcription as measured with LEE-lacZ fusions. Replacement of either cysteine residue 136 or 155 of EscC w...
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PMID: 17933489
PDF is available here.
PDF is available here.
Abstract
This study was conducted to evaluate the efficacy of Saccharomyces boulardii in acute diarrhea. One hundred hospitalized children in Myanmar (age range = 3 months to 10 years) were included. Fifty were treated with S. boulardii for five days in addition to oral rehydration solution (ORS) and 50 were...
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PMID: 18256417
PDF is available here.
PDF is available here.