Christina Frank,
Dirk Werber,
Jakob P Cramer,
Mona Askar,
Mirko Faber,
Matthias an der Heiden,
Helen Bernard,
Angelika Fruth,
Rita Prager,
Anke Spode,
Maria Wadl,
Alexander Zoufaly,
Sabine Jordan,
Markus J Kemper,
Per Follin,
Luise Müller,
Lisa A King,
Bettina Rosner,
Udo Buchholz,
Klaus Stark,
Gérard Krause and
HUS Investigation Team
Abstract
We describe an outbreak of gastroenteritis and the hemolytic-uremic syndrome caused by Shiga-toxin-producing Escherichia coli in Germany in May, June, and July, 2011. The consumption of sprouts was identified as the most likely vehicle of infection.
We analyzed data from reports in G...
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PMID: 21696328
PDF is available here.
Udo Buchholz,
Helen Bernard,
Dirk Werber,
Merle M Böhmer,
Cornelius Remschmidt,
Hendrik Wilking,
Yvonne Deleré,
Matthias an der Heiden,
Cornelia Adlhoch,
Johannes Dreesman,
Joachim Ehlers,
Steen Ethelberg,
Mirko Faber,
Christina Frank,
Gerd Fricke,
Matthias Greiner,
Michael Höhle,
Sofie Ivarsson,
Uwe Jark,
Markus Kirchner,
Judith Koch,
Gérard Krause,
Petra Luber,
Bettina Rosner,
Klaus Stark and
Michael Kühne
Abstract
We conducted a matched case-control study and a recipe-based restaurant cohort study, along with environmental, trace-back, and trace-forward investigations, to determine the source of infection.
The case-control study included 26 case subjects with the hemolytic-uremic syndrome and...
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PMID: 22029753
PDF is available here.
Christina Frank,
Dirk Werber,
Jakob P Cramer,
Mona Askar,
Mirko Faber,
Matthias an der Heiden,
Helen Bernard,
Angelika Fruth,
Rita Prager,
Anke Spode,
Maria Wadl,
Alexander Zoufaly,
Sabine Jordan,
Markus J Kemper,
Per Follin,
Luise Müller,
Lisa A King,
Bettina Rosner,
Udo Buchholz,
Klaus Stark,
Gérard Krause and
HUS Investigation Team
Abstract
We describe an outbreak of gastroenteritis and the hemolytic-uremic syndrome caused by Shiga-toxin-producing Escherichia coli in Germany in May, June, and July, 2011. The consumption of sprouts was identified as the most likely vehicle of infection.
We analyzed data from reports in G...
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PMID: 21696328
PDF is available here.
Abstract
We determined that mycotoxins and STECs were part of the disease complex for dairy cattle with Jejunal Hemorrhage Syndrome (JHS). For STECs to play a role in the development of JHS, we hypothesized that STEC colonization should also be evident in beef cattle with JHS. Aggressive medical and surgical...
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PMID: 21639911
PDF is available here.
Abstract
We examined the role of 2 effectors, Tir and NleB, and the structural translocator component EspA in the adherence of STEC to epithelial cells and in the colonization of cattle. Isogenic deletion mutants were constructed and using microscopy and flow cytometry compared to the wild-type strain in the...
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PMID: 20921984
PDF is available here.
Abstract
We investigated the prevalence of eibG among STEC, the phylogeny of eibG, and eibG allelic variations and their impact on the adherence phenotype. eibG was found in 15.0% of 240 eae-negative STEC strains but in none of 157 eae-positive STEC strains. The 36 eibG-positive STEC strains belonged to 14 s...
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PMID: 20547747
PDF is available here.
Abstract
We developed a completely homogeneous duplex loop-mediated isothermal amplification (LAMP) method. The present LAMP method employed a combination of a 6-carboxyfluorescein (FAM)-labeled primer (donor) for one target gene, a non-labeled primer for the other, and an intercalator ethidium bromide (EtBr...
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PMID: 20230890
PDF is available here.
Abstract
We investigated the hypothesis that flagellin acts in conjunction with Shiga toxin to augment this chemokine expression. We investigated the relative contributions of nuclear factor kappaB (NF-kappaB) and mitogen-activated protein kinase (MAPK) signaling to transcription and translation of interleuk...
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PMID: 20439475
PDF is available here.
Sylvie Nathanson,
Thérésa Kwon,
Monique Elmaleh,
Marina Charbit,
Emma Allain Launay,
Jérôme Harambat,
Muriel Brun,
Bruno Ranchin,
Flavio Bandin,
Sylvie Cloarec,
Guylhene Bourdat-Michel,
Christine Piètrement,
Gérard Champion,
Tim Ulinski and
Georges Deschênes
Abstract
We report a retrospective multicenter series of 52 patients with severe initial neurologic involvement that occurred in the course of D+HUS.
Verotoxigenic Escherichia coli infection was documented in 24. All except two patients had acute renal failure that required peritoneal dialysi...
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PMID: 20498239
PDF is available here.
Abstract
Quantify contamination by verotoxin-producing Escherichia coli associated with hemolytic uremic syndrome (VTEC-HUS) in cattle carcasses and generate estimates of exposure in three likely scenarios.
A model was constructed of the frequency and magnitude of VTEC-HUS contamination from primary producti...
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PMID: 20721440
PDF is available here.
Abstract
These results suggest that deficiency in cellulose production did not alter the serological property, PFGE profile, and growth characteristics of selected STEC strains under optimal growth conditions. The STEC strains and their cellulose-deficient derivates could be useful for studying the role of c...
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PMID: 20537258
PDF is available here.
Abstract
We investigated the prevalence of L. monocytogenes, Salmonella spp., verotoxigenic E. coli (VTEC) and coliforms in 108 asazuke samples that randomly collected from supermarkets in Obihiro (Hokkaido prefecture, Japan) during the period of June to November 2007. Twelve (11.11%) L. monocytogenes were i...
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PMID: 20388574
PDF is available here.
Abstract
Shiga toxin-producing Escherichia coli (STEC) is an important foodborne pathogen, and ruminants are recognized as the main natural reservoir. The purposes of this study were to detect E. coli O157 in bovine feces and surface water in a beef cattle farm of Gualeguaychú, Argentina; to...
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PMID: 20092405
PDF is available here.
Abstract
In November 2008, a case of Shiga toxin-producing Escherichia coli (STEC) infection was reported to the Brisbane Southside Public Health Unit. The case had participated in a school camp. Subsequent investigations confirmed 5 other asymptomatic cases among camp attendees or visitors. Examination of t...
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PMID: 20521500
PDF is available here.
Abstract
We found that acid-resistant STEC survived at higher rates during prolonged incubation in rumen fluid than acid-sensitive STEC and that they resisted the highly acidic conditions of the abomasum fluid, whereas acid-sensitive strains were killed. However, transit through the rumen contents allowed ac...
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PMID: 19948865
PDF is available here.
Abstract
Cattle are considered to be an important reservoir of enterohaemorrhagic Escherichia coli (EHEC) and verotoxigenic Escherichia coli (VTEC) strains that can cause disease in humans, and numerous studies of the prevalence of these strains in cattle (focusing mainly on dairy and beef cattle) have been...
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PMID: 20731320
PDF is available here.
Abstract
Escherichia coli was isolated from the feces of 122 piglets with diarrhea on 55 farms in Korea. The virulence genes of each isolate were characterized by polymerase chain reaction (PCR). Of the 562 isolates, 191 carried 1 or more of the virulence genes tested for in this study. Of the 191 isolates,...
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PMID: 20357961
PDF is available here.
Abstract
Rapid and specific detection of Shiga toxin-producing Escherichia coli (STEC) strains with a high level of virulence for humans has become a priority for public health authorities. This study reports on the development of a low-density macroarray for simultaneously testing the genes stx1, stx2, eae,...
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PMID: 19880649
PDF is available here.
Abstract
Food is an important vehicle for transmission of Shiga toxin-producing Escherichia coli (STEC). To assess the potential public health impact of STEC in Swiss raw milk cheese produced from cow's, goat's, and ewe's milk, 1,422 samples from semihard or hard cheese and 80 samples from soft cheese were e...
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PMID: 20051209
PDF is available here.
Azucena Mora,
Miguel Blanco,
Denise Yamamoto,
Ghizlane Dahbi,
Jesús E Blanco,
Cecilia López,
María P Alonso,
Mônica A M Vieira,
Rodrigo T Hernandes,
Cecilia M Abe,
Roxane M F Piazza,
David W Lacher,
Waldir P Elias,
Tania A T Gomes and
Jorge Blanco
Abstract
A collection of 69 eae-positive strains expressing 29 different intimin types and eight tir alleles was characterized with respect to their adherence patterns to HeLa cells, ability to promote actin accumulation in vitro, the presence of bfpA alleles in positive strains, and bundle-forming pilus (BF...
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PMID: 20112229
PDF is available here.
Abstract
Universal preenrichment broth (UPB) was compared with modified Escherichia coli broth with novobiocin (mEC+n) for enrichment of Shiga toxin-producing E. coli O157 and O26, and with buffered peptone water (BPW) for preenrichment of Salmonella enterica. Ten strains each of the three pathogens were ino...
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PMID: 19833028
PDF is available here.
Abstract
Three methods for enrichment of Shiga toxin-producing Escherichia coli (STEC) were compared using waste pit samples from swine production facilities housing 50 to 3,000 animals. The STEC gene stx2 was detected in 5 of 17 pooled samples using a U.S. Department of Agriculture (USDA) enrichment procedu...
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PMID: 19777903
PDF is available here.
Abstract
Shiga toxin (Verocytotoxin)-producing Escherichia coli (STEC or VTEC) causes serious gastrointestinal infections in humans, including diarrhea and hemorrhagic colitis, and may lead to life-threatening sequelae such as the hemolytic uremic syndrome (HUS). The triennial 'VTEC' meetings...
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PMID: 19681694
PDF is available here.
Abstract
Shiga toxin (Verocytotoxin)-producing Escherichia coli (STEC or VTEC) causes serious gastrointestinal infections in humans, including diarrhea and hemorrhagic colitis, and may lead to life-threatening sequelae such as the hemolytic uremic syndrome (HUS). The triennial 'VTEC' meetings...
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PMID: 19681694
PDF is available here.
Abstract
Shiga toxin (Verocytotoxin)-producing Escherichia coli (STEC or VTEC) causes serious gastrointestinal infections in humans, including diarrhea and hemorrhagic colitis, and may lead to life-threatening sequelae such as the hemolytic uremic syndrome (HUS). The triennial 'VTEC' meetings...
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PMID: 19681694
PDF is available here.
Abstract
The effects of Quercus infectoria (family Fagaceae) nutgalls on cell surface properties of Shiga toxigenic Escherichia coli (STEC) were investigated with an assay of microbial adhesion to hydrocarbon. The surface of bacterial cells treated with Q. infectoria exhibited a higher level of cell surface...
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PMID: 19722403
PDF is available here.
Vanessa V Lorusso,
Angela A Dambrosio,
Nicoletta Cristiana NC Quaglia,
Antonio A Parisi,
Giovanna G La Salandra,
Giuseppe G Lucifora,
Giuseppina G Mula,
Sebastiano S Virgilio,
Leonardo L Carosielli,
Addolorata A Rella,
Marco M Dario and
Giovanni G Normanno
Abstract
Escherichia coli 026 is known as a verocytotoxin-producing E. coli (VTEC) organism that causes severe foodborne diseases such as hemorrhagic colitis and hemolytic uremic syndrome. Although cattle are the most important reservoir of VTEC, only a few reports on the role of water buffalo (Bubalus bubal...
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PMID: 19722404
PDF is available here.
Abstract
We recruited 43 case patients infected with STEC serogroup O157, 71 case patients infected with non-O157 serogroups, and 304 control subjects. One patient infected with serogroup O157 and 7 infected with non-O157 serogroups developed hemolytic uremic syndrome. Compared with control subjects, case pa...
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PMID: 19522658
PDF is available here.
Abstract
In a prospective matched case-control study of sporadic pediatric hemolytic uremic syndrome related to Shiga-toxin producing Escherichia coli infection in France, eating undercooked ground beef, contact with a person with diarrhea, and drinking well water during the summer period were identified as...
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PMID: 19483660
PDF is available here.
Abstract
Overall, during the period of April 2003 to May 2004, a total of 1399 food handlers stool samples were collected and analysed. EPEC expressing the eaeA gene and STEC expressing the stx2 gene were detected in 11/1399 (0.8%) and 2/1399 (0.1%) of the study subjects respectively. The mean age of the sub...
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PMID: 20358790
PDF is available here.
Abstract
Stx2e-treated cells showed characteristic features of apoptosis, including membrane blebbing, DNA fragmentation, chromatin condensation, and the formation of apoptotic bodies, whereas ricin did not induce apoptosis of Vero cells even at a high dose. Fluorescent dye staining showed that Stx2e induced...
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PMID: 19637576
PDF is available here.
Abstract
We genetically lack the necessary enzyme. However, Neu5Gc can still be found on human cells due to metabolic incorporation from the diet. Dietary incorporation happens to be highest in human endothelium and to a lesser extent in the intestinal epithelium, the two affected cell types in STEC-induced...
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PMID: 19387473
PDF is available here.
Abstract
The stable attenuated Salmonella choleraesuis vaccine strain expressing SLT-IIeB and FedF of SLTEC O138 was constructed with balanced lethal system. The expressed products with protein quality 37000 could react with the antibody of FedF and SLT-IIeB. CONCLUSION: The attenuated Salmonella choleraesui...
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PMID: 19621641
PDF is available here.
Abstract
The objective of this study was to identify individual cattle-level risk factors associated with fecal shedding of Shiga toxin-encoding bacteria (STB), a surrogate for Shiga toxin-producing E. coli (STEC), on 28 organic and conventional dairy farms. It was found that small organic herds (fewer than...
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PMID: 19436585
PDF is available here.
Abstract
The prevalence of STEC in Japan was examined using rectal stool samples taken from 932 healthy dairy cows from 123 farms in 11 prefectures between 2006 and 2007. Screening with stx-PCRs revealed the prevalence to be 30.4% (283 animals), and STEC strains were isolated from 111 animals. Although ten O...
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PMID: 19346709
PDF is available here.
Abstract
We compared the genetic composition of the virulence plasmids pO113 and pO157 from LEE-negative and LEE-positive STEC, respectively. The complete nucleotide sequence of pO113 showed that several plasmid genes were shared by STEC O157:H7. In addition, allelic profiling of the ehxA gene demonstrated t...
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PMID: 19239748
PDF is available here.
Abstract
To provide information on the persistence and maintenance of colonization with Shiga toxin-producing Escherichia coli (STEC) in sheep, pulsed-field gel electrophoresis analysis of STEC isolates (n = 145) belonging to serogroups O5, O91, and O146 from 39 healthy animals was performed in a 12-month lo...
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PMID: 19168649
PDF is available here.
Abstract
Studies to determine baseline levels of microbial contaminants and foodborne bacterial pathogens are needed to evaluate the effectiveness of Hazard Analysis Critical Control Point (HACCP) programs, Good Manufacturing/Production Practices, and various interventions. In 2004 and 2005 poultry carcass r...
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PMID: 19412397
PDF is available here.
Abstract
We describe the molecular mechanisms by which VT alters the endothelial cell phenotype in HUS. RNA metabolism pathways and effects on translation may play central roles in the molecular events operative in vascular injury mediated by these potent bacteria-derived exotoxins....
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PMID: 19180125
PDF is available here.
Abstract
The high incidences of waterborne diseases are frequently associated with shiga toxin (STEC) and enterotoxin producing Escherichia coli (ETEC). Therefore, in the present study, surface water samples collected from the river Saryu were analyzed for the presence of multi-antimicrobial resistant ETEC a...
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PMID: 18692897
PDF is available here.
Abstract
Shiga toxin (Stx)-producing Escherichia coli (STEC) cause an enteric illness that results in a spectrum of outcomes ranging from asymptomatic carriage to uncomplicated diarrhea, bloody diarrhea, and the postdiarrheal haemolytic uremic syndrome (HUS), which leads to renal and other organ microvascula...
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PMID: 19190807
PDF is available here.
Abstract
The objective of this study was to compare fecal indicator bacteria (FIB) (fecal coliforms, Escherichia coli [EC], and enterococci [ENT]) concentrations with a wide array of typical organic wastewater chemicals and selected bacterial genes as indicators of fecal pollution in water samples collected...
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PMID: 19141815
PDF is available here.
Author(s) unavailable
Abstract
For updated information, including data and facts on specific diseases, visit www.health.nsw.gov.au and click on Infectious Diseases or access the site directly at: http://www.health.nsw.gov.au/publichealth/infectious/index.asp.
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PMID: 19261214
PDF is available here.
Abstract
An outbreak of Escherichia coli O157 involving four people in North Wales is currently being investigated. Laboratory typing shows all the isolates belong to phage type 2. All four cases reported eating different products from a fast food outlet in the area. The possibility of other common exposures...
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PMID: 19679034
PDF is available here.
Abstract
We characterized our STEC strains isolated from diarrheal and asymptomatic persons in northern and southwest Iran. The 29 STEC strains were examined for the presence of virulence genes by polymerase chain reaction (PCR) and their H type was analyzed by PCR-restriction fragment length polymorphism (R...
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PMID: 19168953
PDF is available here.
Abstract
Our data illustrate that cattle are a potential source of some atypical antibiotic-resistant E. coli isolates that harbor virulence genes....
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PMID: 19205460
PDF is available here.
Abstract
This study was undertaken to characterize the integrons present in a group of Shiga toxin-producing Escherichia coli (STEC) isolates and the ability of these integrons to transfer antibiotic resistance genes from STEC to E. coli K-12 MG1655. A total of 177 STEC isolates were analyzed for antibiotic...
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PMID: 19205459
PDF is available here.
I Friesema,
G Sigmundsdottir,
K van der Zwaluw,
A Heuvelink,
B Schimmer,
C de Jager,
B Rump,
H Briem,
H Hardardottir,
A Atladottir,
E Gudmundsdottir and
W van Pelt
Abstract
Between 14 September and 20 October 2007, an outbreak of Shiga toxin-producing Escherichia coli (STEC) O157 simultaneously occurred in the Netherlands and Iceland. A total of 50 laboratory-confirmed cases were reported with a STEC O157 infection caused by the same clone. The strain was of type O157:...
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PMID: 19087865
PDF is available here.
Emma Byres,
Adrienne W Paton,
James C Paton,
Jonas C Löfling,
David F Smith,
Matthew C J Wilce,
Ursula M Talbot,
Damien C Chong,
Hai Yu,
Shengshu Huang,
Xi Chen,
Nissi M Varki,
Ajit Varki,
Jamie Rossjohn and
Travis Beddoe
Abstract
We show that SubB has a strong preference for glycans terminating in the sialic acid N-glycolylneuraminic acid (Neu5Gc), a monosaccharide not synthesized in humans. Structures of SubB-Neu5Gc complexes revealed the basis for this specificity, and mutagenesis of key SubB residues abrogated in vitro gl...
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PMID: 18971931
PDF is available here.
Abstract
mean age 16 months, bloody diarrhea 57.8%, no previous diarrhea 9%, 60% received antibiotics, 72% had oligoanuria, 53% required dialysis, 15% had seizures and 31% had dizziness; mortality was 3%. Four foodborne outbreaks have been detected in Santiago, two outbreaks occurred in household settings, o...
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PMID: 19194606
PDF is available here.
Abstract
Healthy ruminants carry intestinal Shiga toxin (Stx)-producing Escherichia coli (STEC). Stx has antiviral activities in vitro and STEC numbers correlate with reduced early viremia in sheep experimentally infected with bovine leukemia virus (BLV). This study assessed the impact of intestinal STEC on...
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PMID: 19043312
PDF is available here.