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Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Escherichia coli (4):
Recent article
Escherichia coli
Abstract
We demonstrate that a single, site-specific, cyclobutane pyrimidine dimer leading-strand template lesion provides only a transient block to fork progression in vitro. The replisome remains stably associated with the fork after collision with the lesion. Leading-strand synthesis is then reinitiated d...
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PMID: 21998391
PDF is available here.
PDF is available here.
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Proteomics (11)18 2011
Abstract
We present a simple, time- and cost-efficient approach to tackle the proteome of prokaryotic organisms. To obtain large data sets of complex biological experiments high-throughput and time- and cost-efficient methods still have to be developed and refined. In this study, we combined well-approved te...
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PMID: 21751368
PDF is available here.
PDF is available here.
Abstract
We demonstrate that a functional reversal of the β-oxidation cycle can be used as a metabolic platform for the synthesis of alcohols and carboxylic acids with various chain lengths and functionalities. This pathway operates with coenzyme A (CoA) thioester intermediates and directly uses acetyl-CoA...
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PMID: 21832992
PDF is available here.
PDF is available here.
Ukrainskij biokhimicheskij zhurnal/Ukrayins'kij biokhimichnij zhurnal/Ukrainian biochemical journal (82)6 2011
Abstract
We suppose that diverse sensitivity to DT of monkey and mouse cells can be explained not only by differences in their receptor affinity for DT but also by the processes that occur after internalization of the toxin into the cells....
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PMID: 21805864
PDF is available here.
PDF is available here.
Abstract
Abstract
Fibrillar aggregates of beta-amyloid peptide (Abeta) are major constituents of the senile plaques found in the brains of patients suffering from Alzheimer's disease (AD). Previous studies have shown that spontaneous isomerization or racemization of aspartyl residues in Abeta peptides...
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PMID: 21812329
PDF is available here.
PDF is available here.
Biomacromolecules (12)6 2011
Abstract
We have designed and produced a modular, recombinant resilin-like polypeptide bearing both mechanically active and biologically active domains to create novel biomaterial microenvironments for engineering mechanically active tissues such as blood vessels, cardiovascular tissues, and vocal folds. Pre...
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PMID: 21553895
PDF is available here.
PDF is available here.
Cell Host & Microbe (9)6 2011
Abstract
We show that the TLR3/MDA5 agonist poly I:C or viral infection dramatically augments signaling via the NLRs Nod1 and Nod2 and enhances the production of proinflammatory cytokines. Enhanced Nod1 and Nod2 signaling by poly I:C required the TLR3/MDA5 adaptors TRIF and IPS-1 and was mediated by type I I...
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PMID: 21669398
PDF is available here.
PDF is available here.
Abstract
We cloned genes coding for human HMGB1 and its active components A box and B box by PCR and inserted the cloned genes into pET28a vectors for transformation of Escherichia coli BL21. The E. coli expressed proteins were then purified with a Ni(2+)-NTA column and the endotoxin content was removed. Rec...
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PMID: 21255697
PDF is available here.
PDF is available here.
Abstract
We describe and conduct a pilot test of a multiplex sequencing strategy suitable for achieving total reads of > 300 bases per extracted DNA molecule on the IGA. Results show that all proposed primers produce products of the expected size and that correct sequences can be obtained, with all propo...
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PMID: 21361774
PDF is available here.
PDF is available here.
PLoS Biology (9)6 2011
Abstract
We employ population genomics data from Escherichia coli, Saccharomyces paradoxus, and Drosophila to provide evidence suggesting that it is not the indels per se but the sequence in which indels occur that causes the accumulation of nucleotide substitutions. We found that about two-thirds of indels...
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PMID: 21697975
PDF is available here.
PDF is available here.
Abstract
We have asked Mario Marcon of Nationwide's Children Hospital in Columbus, OH, to explain the rationale for his decision to follow this recommendation, while we have asked Deanna Kiska and Scott Riddell of Upstate University Hospital in Syracuse, NY, why these guidelines have not been adopted by thei...
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PMID: 21543568
PDF is available here.
PDF is available here.
Xenobiotica (41)5 2011
Abstract
Tyrosine phosphorylation plays a major role in intracellular signal transduction pathways. Phosphorylated tyrosine residues initiate signaling pathways by recruiting proteins containing Src homology-2 (SH2) domains. Herein is described a high-throughput assay to detect interactions between phosphory...
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PMID: 18370317
PDF is available here.
PDF is available here.
Xenobiotica (41)5 2011
Abstract
The production of recombinant proteins usually involves the exploration of a wide variety of expression and purification methodologies in the pursuit of a strategy tailored to a particular protein. The methods applied are reliant on exploiting individual differences between expression systems or the...
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PMID: 18988024
PDF is available here.
PDF is available here.
Xenobiotica (41)5 2011
Abstract
Key enzymes that assemble the bacterial cell wall are also the target of the Beta-lactam class of antibiotics. The covalent binding of labeled penicillin to these proteins has been used in numerous studies in drug discovery, antibiotic mechanisms of action and resistance, and cell wall physiology. M...
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PMID: 18437311
PDF is available here.
PDF is available here.
Xenobiotica (41)5 2011
Abstract
A method is described for generating conditional lethal mutations in essential genes in Escherichia coli. In this procedure, amber stop codons are introduced as "tagalong" mutations in the flanking DNA of a downstream antibiotic-resistance marker by lambda Red recombination. The marker is removed by...
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PMID: 18392977
PDF is available here.
PDF is available here.
Abstract
Background & objectives : Limited information is available on shiga toxin producing Escherichia coli (STEC) in animals and birds from India. An outbreak of acute diarrhoea in poultry birds at Aizawl, Mizoram was investigated for detection and characterization of STEC and enteropathogenic E. coli (EPE...
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PMID: 21623041
PDF is available here.
PDF is available here.
Abstract
We report the crystal structure of the Cdc25 domain of RalGPS1a. The bowl shaped structure is homologous to the Cdc25 domains of SOS and RasGRF1. The most remarkable difference between these three Cdc25 domains lies in their active sites, referred to as the helical hairpin region. Consistent with pr...
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PMID: 21494904
PDF is available here.
PDF is available here.
Proteomics (11)7 2011
Abstract
We report the subproteome reference maps of E. coli B REL606 by analyzing cytoplasmic, periplasmic, inner and outer membrane, and extracellular proteomes based on the genome information using experimental and computational approaches. Among the total of 3487 spots, 651 proteins including 410 non-red...
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PMID: 21337514
PDF is available here.
PDF is available here.
Abstract
Current Biology (21)5 2011
Abstract
We found that yeast and mammalian formins bind actin monomers but that this activity requires their C-terminal DAD domains. Furthermore, we observed that the DAD works in concert with the FH2 to enhance nucleation without affecting the rate of filament elongation. We dissected this mechanism in mDia...
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PMID: 21333540
PDF is available here.
PDF is available here.
Systems Biology, IET (5)2 2011
Abstract
Many methods had been developed on inferring transcriptional network from gene expression. However, it is still necessary to design new method that discloses more detailed and exact network information. Using network-assisted regression, the authors combined the averaged three-way mu...
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PMID: 21405197
PDF is available here.
PDF is available here.
Abstract
We show simultaneous binding of FH19 to C3b and FH20 to nonactivator surface glycosaminoglycans, and we show that both of these interactions are necessary for full binding of FH to C3b on nonactivator surfaces (i.e., for target discrimination). We also show that C3d could replace glycosaminoglycan b...
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PMID: 21285368
PDF is available here.
PDF is available here.
ISME Journal (5)2 2011
Abstract
We describe here the role of muramidases present in clones of metagenomic DNA that result in cell aggregation and biofilm formation by Escherichia coli. The metagenomic clones were obtained from uncultured Lachnospiraceae-affiliated bacteria resident in the foregut microbiome of the Tammar wallaby....
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PMID: 20668486
PDF is available here.
PDF is available here.
Proteomics (11)3 2011
Abstract
Cell surface physicochemical characterization techniques were combined with quantitative changes in protein expression, to investigate the biological and biophysical changes of Escherichia coli MG1655 cells when grown as a biofilm (BIO). The overall surface charge of BIO cells was fo...
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PMID: 21268264
PDF is available here.
PDF is available here.
Abstract
Escherichia coli DH5α grows poorly in a chemically defined medium, but its acetate-tolerant mutant, DA19, grows fast and produces less acetate. To investigate the differences in metabolism between the two strains, the samples of continuous cultures at steady state were analyzed by t...
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PMID: 20700606
PDF is available here.
PDF is available here.
Nature Protocols (6)2 2011
Abstract
We provide a protocol for a sequence-independent approach for cloning complex individual or combinatorial DNA libraries, and routine or high-throughput cloning of single or multiple DNA fragments. The strategy, called circular polymerase extension cloning (CPEC), is based on polymerase overlap exten...
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PMID: 21293463
PDF is available here.
PDF is available here.
Epidemiology and Infection (139)2 2011
Abstract
These results can inform management of canine patients and infection control procedures to mitigate the risk of clinical disease due to MDR bacteria in hospitalized dogs....
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PMID: 20392305
PDF is available here.
PDF is available here.
Abstract
In this study agarose-alginate scaffolds are synthesized using cryogelation technology in different formats like monolith, sheet, discs, and beads, and show amiable mechanical strength like soft tissue properties and high interconnected macroporous degradable architecture. In cell-ma...
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PMID: 21077225
PDF is available here.
PDF is available here.
Abstract
We demonstrate that TAX1BP1 was inducibly phosphorylated on Ser593 and Ser624 in response to proinflammatory stimuli. The kinase IKKα, but not IKKβ, was required for phosphorylation of TAX1BP1 and directly phosphorylated TAX1BP1 in response to stimulation with tumor necrosis factor (TNF) or interl...
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PMID: 21765415
PDF is available here.
PDF is available here.
Abstract
Abstract
We use global quantitative proteomics to profile the response to infection with E. coli strain LF82 isolated from patients suffering from Crohn's disease, an inflammatory bowel disease. We show that LF82 infection induces more than one hundred proteins. The response share many functional categories...
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PMID: 21389771
PDF is available here.
PDF is available here.
Abstract
We demonstrate that RNAi also functions to repress genomic elements other than constitutive heterochromatin. Using DNA adenine methyltransferase identification (DamID), we show that the RNAi proteins Dcr1 and Rdp1 physically associate with some euchromatic genes, noncoding RNA genes and retrotranspo...
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PMID: 21151114
PDF is available here.
PDF is available here.
Metallomics (3)1 2011
Abstract
From the reaction of 1-methylimidazole (1a), 4,5-dichloro-1H-imidazole (1b(I)) and 1-methylbenzimidazole (1c) with p-cyanobenzyl bromide (2a), non-symmetrically substituted N-heterocyclic carbene (NHC) [(3a-c)] precursors, 5,6-dimethyl-1H-benzimidazole (1d) and 4,5-diphenyl-1H-imidaz...
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PMID: 21135954
PDF is available here.
PDF is available here.
Abstract
These results demonstrate that oxygen-generating nanofibers can be designed and developed to provide a short-term peroxide-based antimicrobial response while still maintaining attractive tissue-integration properties....
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PMID: 21155527
PDF is available here.
PDF is available here.
Abstract
We present a protocol using an Escherichia coli strain equipped with a luxR-based monitor system, which in the presence of exogenous AHL molecules expresses a green fluorescent protein (GFP). Lungs from mice challenged intratracheally with alginate beads containing both a P. aeruginosa strain togeth...
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PMID: 21031310
PDF is available here.
PDF is available here.
Abstract
We describe a methodology to heterologously express, purify, and conduct in vitro characterization of several metalloforms of the AHL lactonase from Bacillus thuringiensis (AiiA). These procedures should be applicable to similar enzymes and will facilitate the production of more useful quorum-quench...
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PMID: 21031319
PDF is available here.
PDF is available here.
Abstract
Rubisco activase is a molecular chaperone that modulates the activation state of Rubisco by catalyzing the ATP-dependent removal of tightly-bound inhibitory sugar-phosphates from Rubisco's catalytic sites. This chapter reports methods developed for the purification of native and recombinant Rubisco...
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PMID: 20960143
PDF is available here.
PDF is available here.
Abstract
We describe efficient procedures for the isolation of highly purified His-tagged target proteins from an Escherichia coli host using IMAC....
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PMID: 20978973
PDF is available here.
PDF is available here.
Abstract
Plastocyanin and cytochrome c6 are widely distributed over the oxygen-evolving photosynthetic organisms. The two proteins are functionally equivalent, but strongly differ in their global electrostatic charge. In fact, they are acidic in eukaryotes, but either neutral or basic in cyanobacteria. Such...
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PMID: 20960123
PDF is available here.
PDF is available here.
Abstract
We and others recently observed that lipopolysaccharide (LPS) administration, as other TLR ligands, induces MDSC. In this case, MDSC regulate immune response independently of L-arginine metabolism through heme oxygenase-1 activity. Manipulating MDSC as immunoregulators represents an attractive appro...
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PMID: 20941610
PDF is available here.
PDF is available here.
Abstract
This chapter describes the use of glutathione S-transferase (GST) gene fusion proteins as a method for inducible, high-level protein expression and purification from bacterial cell lysates. The protein is expressed in a pGEX vector, with the GST moiety located at the N terminus followed by the targe...
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PMID: 20978970
PDF is available here.
PDF is available here.
Abstract
Maltose-binding protein (MBP) is one of the most popular fusion partners being used for producing recombinant proteins in bacterial cells. MBP allows one to use a simple capture affinity step on amylose-agarose columns, resulting in a protein that is often 70-90% pure. In addition to protein-isolati...
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PMID: 20978971
PDF is available here.
PDF is available here.
Abstract
Our results indicate that mcrA and pmoA copy numbers vary during composting and that methanogen and methanotroph populations need to be examined in conjunction with net CH4 emissions from open-windrow composting of cattle feedlot manure....
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PMID: 21488508
PDF is available here.
PDF is available here.
Abstract
The silica-silver core-shell particles were synthesized by simple one pot chemical method and were employed on the cotton fabric as an antibacterial agent. Extremely small (1-2 nm) silver nanoparticles were attached on silica core particles of average 270 nm size. The optimum density of the nano sil...
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PMID: 20864320
PDF is available here.
PDF is available here.
Thrombosis and Haemostasis (105)1 2011
Abstract
We aimed to establish a porcine model with haemorrhagic shock with consecutive sepsis and hypothesised that a decreased ADAMTS13-activity as well as an altered VWF multimer pattern is associated with renal failure. Animals (n=21) were subjected to haemorrhagic shock. After volume replacement, intrap...
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PMID: 21057705
PDF is available here.
PDF is available here.
Abstract
Evaluation of immunobiological properties of recombinant atoxic forms of the Pseudomonas aeruginosa exotoxin. MATERIALS AND METHODS. 3 recombinant atoxic forms of the P. aeruginosa exotoxin A were produced and studied: aTox1, consisting only of exotoxin A domain 1; aTox1,2, consisting of domain 1 and...
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PMID: 21598613
PDF is available here.
PDF is available here.
Abstract
We speculated a functional corelation between bacterial protelolytic apparatus and its anti-oxidative system. This prediction may contribute to our better understanding of functional relationship between proteolytic and antioxidative systems in biological worlds in the future investigations....
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PMID: 21950114
PDF is available here.
PDF is available here.
Abstract
Abstract
On July 6, 2009, the Organ Procurement and Transplantation Network received notification of possible disease transmission. A transplant center in California (TCA) reported a kidney transplant recipient with Escherichia coli urinary tract infection and sepsis suspected to have been contracted from th...
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PMID: 21178948
PDF is available here.
PDF is available here.
Lab On a Chip (10)23 2010
Abstract
We demonstrate for the first time continuous DNA extraction from cell lysate on a microfluidic chip. After mixing bacterial Escherichia coli culture with superparamagnetic bead suspension, lysis and binding buffers, DNA is released from cells and captured by the beads. These DNA carrying beads are c...
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PMID: 20938545
PDF is available here.
PDF is available here.
Lab On a Chip (10)23 2010
Abstract
We report a low cost, disposable polymer microfluidic sample preparation device to perform rapid concentration of bacteria from liquid samples using enhanced evaporation targeted at downstream detection using surface enhanced Raman spectroscopy (SERS). The device is composed of a poly(dimethylsiloxa...
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PMID: 20938505
PDF is available here.
PDF is available here.
Abstract
We examined whether catheterizing ileal loops in sheep affected the overall health of animals and intestinal function, whether a bacterial treatment could be introduced into the loops through the catheters, and whether broad-spectrum antibiotics could sterilize the loops. Escherichia coli cells tran...
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PMID: 21262134
PDF is available here.
PDF is available here.
Microbial Pathogenesis (49)6 2010
Abstract
Coxiella burnetii, an obligate intracellular Gram-negative bacterium, is the etiological agent of Q fever. This work takes advantage of a hypersensitive Escherichia coli genetic system to identify genes involved in resistance to nitrosative stress imposed by reactive nitrogen intermediates. Among th...
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PMID: 20705129
PDF is available here.
PDF is available here.
Lancet Infectious Diseases (10)12 2010
Abstract
Epidemiology and Infection (138)12 2010
Abstract
A systematic review of outbreak and non-outbreak studies of infections caused by extraintestinal pathogenic Escherichia coli (ExPEC) was conducted. This review examines the epidemiology, seasonality, source or mode of transmission, and temporal changes, based on E. coli serogroup, in ExPEC causing s...
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PMID: 20642873
PDF is available here.
PDF is available here.
EMBO Journal (29)23 2010
Abstract
We found that, in the rate-limiting intermediate of a chaperonin reaction, the unfolded polypeptide in the cage partly protrudes through a narrow space near the GroEL/GroES interface. Then, the entire polypeptide is released either into the cage or to the outside medium. The former adopts a native s...
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PMID: 20959808
PDF is available here.
PDF is available here.
EMBO Journal (29)23 2010
Abstract
We show the presence of a transient dwell between F₀ subunits a and c by improving the time resolution to 10 μs at unprecedented S/N, and by using Escherichia coli F₀F₁ embedded in lipid bilayer nanodiscs. The transient dwell interaction requires 163 μs to form and 175 μs to dissociate, is...
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PMID: 21037553
PDF is available here.
PDF is available here.
Mutation Research (711)1-2 2011
Abstract
A signature of ionizing radiation exposure is the induction of DNA clustered damaged sites, defined as two or more lesions within one to two helical turns of DNA by passage of a single radiation track. Clustered damage is made up of double strand breaks (DSB) with associated base lesions or abasic (A...
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PMID: 21130102
PDF is available here.
PDF is available here.
Abstract
We present structural characterization of oxidation intermediates for these demethylases. Using a chemical cross-linking strategy, complexes of AlkB-double stranded DNA (dsDNA) containing 1,N(6)-etheno adenine (εA), N(3)-methyl thymine (3-meT) and N(3)-methyl cytosine (3-meC) are stabilized and cry...
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PMID: 21068844
PDF is available here.
PDF is available here.