Abstract
We are born, we are exposed to a vast variety of microbes. The intestine in particular is perhaps inhabited by the largest number of microbes, consisting of both established commensals as well as sporadic pathogens. Mucosal surfaces form an important barrier against microbial invasion. Together with...
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PMID: 20083498
PDF is available here.
Abstract
We conducted a survey among 29 European countries (the European Union Member States, Iceland and Norway) to gather information on the spread of NDM-1-producing Enterobacteriaceae in Europe, on public health responses and on available national guidance on detection, surveillance and control. A total...
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PMID: 21144431
PDF is available here.
H Grundmann,
D M Livermore,
C G Giske,
R Canton,
G M Rossolini,
J Campos,
A Vatopoulos,
M Gniadkowski,
A Toth,
Y Pfeifer,
V Jarlier,
Y Carmeli and
CNSE Working Group
Abstract
The emergence and global spread of carbapenemase-producing Enterobacteriaceae is of great concern to health services worldwide. These bacteria are often resistant to all beta-lactam antibiotics and frequently co-resistant to most other antibiotics, leaving very few treatment options. The epidemiolog...
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PMID: 21144429
PDF is available here.
Abstract
We show that the CDI growth inhibitory activity resides within the carboxy-terminal region of CdiA (CdiA-CT), and that CdiI binds and inactivates cognate CdiA-CT, but not heterologous CdiA-CT. Bioinformatic and experimental analyses show that multiple bacterial species encode functional CDI systems...
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PMID: 21085179
PDF is available here.
Wendy S Garrett,
Carey A Gallini,
Tanya Yatsunenko,
Monia Michaud,
Andrea DuBois,
Mary L Delaney,
Shivesh Punit,
Maria Karlsson,
Lynn Bry,
Jonathan N Glickman,
Jeffrey I Gordon,
Andrew B Onderdonk and
Laurie H Glimcher
Abstract
We characterized the fecal microbial communities in the recently described T-bet(-/-) ×Rag2(-/-) ulcerative colitis (TRUC) model driven by T-bet deficiency in the innate immune system. 16S rRNA-based analysis of TRUC and Rag2(-/-) mice revealed distinctive communities that correlate with host genot...
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PMID: 20833380
PDF is available here.
Abstract
We evaluated the ATAD process over a period of one year in a two stage, full scale Irish ATAD plant established in Killarney and treating mixed primary and secondary sludge, by examining the sludge microbiologically at various stages during and following ATAD processing to determine its ability to e...
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PMID: 20948933
PDF is available here.
Abstract
We suggest that an appropriate period of time for using disinfected cleaning cloths is around two hours, not exceeding three hours of usage....
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PMID: 21045369
PDF is available here.
Abstract
The mode of the transmission of Helicobacterpylori infection remains poorly understood. A total of 198 samples of drinking water from 22 districts of Basra governorate were collected during the period October 2006 to July 2007. The concentration of residual chlorine was measured and the numbers of t...
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PMID: 21218716
PDF is available here.
Abstract
The production of Arbequina naturally green olives is a traditional and spontaneous process in which lactic acid bacteria (LAB) and yeasts are present. To better control the fermentation of olives, strains of LAB and yeasts that had been isolated from brines were used in this study. A strain of Lact...
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PMID: 20630314
PDF is available here.
Abstract
We isolated 4 IMP-1 metallo-beta-lactamase (MBL) producers of this family having transferable carbapenem-resistance markers. When examined with MicroScan Neg-Combo Panels, all 4 showed imipenem-MIC of either <1 microg/mL or 2 microg/mL, although they were highly resistant to ceftazidime (MIC: >16 mi...
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PMID: 20960935
PDF is available here.
Abstract
We show that thiamine monophosphate, the specific thiamine derivative putatively synthesized by Wigglesworthia, impacts Sodalis thiamine transporter expression, proliferation and intracellular localization. A greater understanding of tsetse symbiont interactions may generate alternative control stra...
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PMID: 20356887
PDF is available here.
Abstract
Raoultella planticola has been rarely diagnosed in clinical specimens. A case of a polymicrobial surgical site infection primarily caused by R. planticola in a 66-year-old Caucasian male with a fractured left tibia after an open reduction internal fixation of his left ankle is described and confirms...
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PMID: 20852504
PDF is available here.
Abstract
We provide new insight into the structure-function relationship of the SPATEs and the functional roles of their conserved residues....
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PMID: 20479079
PDF is available here.
Abstract
We think that they could effectively replace MHT for the convenient and early detection of KPC carbapenemases in regions where these enzymes are common....
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PMID: 20395214
PDF is available here.
Abstract
We investigated whether plasmid uptake varied among genetically indistinguishable isolates of Dickeya sp. from a 400 m(2) field plot. The transfer frequencies of broad-host-range IncP-1 plasmids from Escherichia coli to Dickeya differed significantly among isolates. The transfer frequencies for plas...
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PMID: 20455941
PDF is available here.
Abstract
We investigated the regulation of several Sodalis genes that encode putative iron acquisition systems. These genes, SG1538 (hemT) and SG1516 (sitA), are homologous to genes encoding periplasmic heme and iron/manganese transporters, respectively. hemT promoter- and sitA promoter-gfp fusions were cons...
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PMID: 20494987
PDF is available here.
Abstract
We show that mutations in the RcsCDB phosphorelay system restored virulence and motility in a D. dadantii opg-negative strain, indicating a relationship between the Rcs phosphorelay and OPGs....
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PMID: 20418397
PDF is available here.
Abstract
We have identified substantial incongruence between the phylogenies of orthologous genes in Escherichia, Salmonella, and Citrobacter, or E. coli, E. fergusonii, and E. albertii. The source of incongruence was inferred to be recombination, because individual genes support conflicting topology more ro...
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PMID: 20534528
PDF is available here.
Abstract
Small RNAs (sRNAs) are non-coding transcripts exerting their functions in the cells directly. Identification of sRNAs is a difficult task due to the lack of clear sequence and structural biases. Most sRNAs are identified within genus specific intergenic regions in related genomes. Ho...
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PMID: 20691398
PDF is available here.
Abstract
Bragança's socioeconomic situation is highly dependent on estuarine and marine biological resources that are influenced by tidal cycles and climatology. Field measurements (hydrological, hydrodynamic and microbiological variables) were taken in the most urbanized zone from Caeté estuary to charact...
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PMID: 20563427
PDF is available here.
Abstract
These results were strengthened by a longitudinal survey of wing breakage, which showed almost all wing puncturing occurred during the de-feathering process, limiting the likelihood of microbial contamination. Combined, these results indicate there is no increased health risk from consumption of dam...
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PMID: 20417402
PDF is available here.
Abstract
The effect of reduced oxygen atmosphere and sodium acetate treatment on the microbial quality of seer fish (Scomberomorus commerson) steaks was determined during chilled storage (1-2 degrees C). The O2 absorber reduced the oxygen content in the pack to less than 0.01% corresponding to 99.96% reducti...
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PMID: 20417403
PDF is available here.
Abstract
We evaluated the effects of probiotic strains administration in pigs. On the 35th day of age, 30 pigs were distributed into 2 groups: the non-treated control group (initial average BW: 8.3+/-0.6 kg) and a probiotic supplemented fed group (initial average BW: 8.7+/-0.4 kg). Each experimental group wa...
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PMID: 20471591
PDF is available here.
Abstract
We demonstrated that human adenovirus had the highest detection rate (96.3%), followed by somatic coliphages (73.5%), human polyomavirus (68.6%), and rotavirus (63.5%). However, norovirus GII and enterovirus were found in only 25.7 and 17.8%, respectively. The concentration of the viral genome range...
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PMID: 20556879
PDF is available here.
Abstract
We conclude that drinking water contamination in La Plata occurs in the distribution system due to increased temperatures and reduced disinfectant levels, which result in bacterial regrowth....
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PMID: 20154384
PDF is available here.
Abstract
Israel Defense Forces (IDF) guidelines for drinking water require the use of water only from sources that have been inspected and authorized by a medical expert. This study aimed to compare canteen water quality of two military units (infantry and armoured corps), to search for sources of possible m...
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PMID: 20154387
PDF is available here.
Abstract
We studied how wastewater treatment affects the prevalence and concentration of two genes, tetA and tetB, that encode resistance to tetracycline. Using real-time polymerase chain reaction (PCR) we analysed wastewater samples collected monthly for one year at eight key-sites in a full-scale municipal...
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PMID: 20154388
PDF is available here.
Abstract
We detected E. coli in over 2% of samples from 40% (129/323) of systems. E. coli detection was significantly more common in earlier years and during summer (p<0.001). On multivariate analysis E. coli detection was significantly associated with smaller systems; watercourse sources; no disinfection or...
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PMID: 20154389
PDF is available here.
Abstract
We propose limiting summer cattle grazing on public lands to lower elevations, with a final goal of allowing summer grazing on public lands only below 1,500 m elevation in the Central and Northern Sierra and 2,000 m elevation in the Southern Sierra....
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PMID: 20154395
PDF is available here.
Abstract
We wished to assess the prevalence and determine the sources of three zoonotic bacterial pathogens (Salmonella, Campylobacter, and Escherichia coli O157:H7) in the Salmon River watershed in southwestern British Columbia. Surface water, sewage, and animal faecal samples were collected from the waters...
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PMID: 20154400
PDF is available here.
A J Brink,
D Bizos,
K D Boffard,
C Feldman,
D C Grolman,
J Pretorius,
G A Richards,
M Senekal,
E Steyn,
N Welkovic,
Association of Surgeons of South Africa,
Critical Care Society of Southern Africa,
Federation of Infectious Diseases Societies of Southern Africa,
South African Thoracic Society and
Trauma Society of South Africa
Abstract
INTRODUCTION: Tigecycline, the first of a new class of antibiotics, the glycylcyclines, was licensed in South Africa for the parenteral treatment of adult patients with complicated intra-abdominal infections (cIAIs) and complicated skin and soft-tissue infections (cSSTIs). METHODS: A multidisciplina...
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PMID: 20529440
PDF is available here.
Abstract
This study investigated antibiotic resistance profiles and tetracycline resistance genes in Enterobacteriaceae family isolates from the Pearl rivers. The Enterobacteriaceae isolates were tested for susceptibility to seven antibiotics ampicillin, chloramphenicol, ciprofloxacin, levofloxacin, sulphame...
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PMID: 20356660
PDF is available here.
Abstract
Our objective was to determine the risk factors associated with bacteriological quality of bulk tank milk. Bulk tank milk samples were collected from all Prince Edward Island dairy herds (n=235) from March 2005 to March 2007. Biweekly total bacterial, preliminary incubation, laboratory pasteurizatio...
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PMID: 20381889
PDF is available here.
Abstract
This study evaluated the effect of plugging the anuses of pork carcasses prior to scalding and dehairing on Enterobacteriaceae contamination on pork carcass surfaces. Results showed that Enterobacteriaceae counts around the anuses of unplugged carcasses increased by 1.15 log CFU/cm(2) after the scal...
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PMID: 20537268
PDF is available here.
Abstract
The Cronobacter spp., previously known as Enterobacter sakazakii, are Gram-negative enterobacterial pathogens that can cause necrotizing enterocolitis, meningitis, and septicemia with a high mortality rate in neonates. The O-specific polysaccharide (O-PS) was isolated from Cronobacter sakazakii stra...
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PMID: 20206918
PDF is available here.
Abstract
ESBL organisms provide a continuing challenge in the geriatric community. They are increasingly prevalent, and pose unique challenges in treatment. Carbapenems are the mainstay of therapy; however they are expensive medications that require prolonged intravenous administration. Carbapenem resistance...
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PMID: 20533728
PDF is available here.
Abstract
Samples of water and barnacles Amphibalanus amphitrite were collected from Recife, Brazil, to assess if it accumulates total (TC) and thermotolerant coliforms (TTC) related with sewage pollution. The Most Probable Number (MPN) values and the standard procedures for examination of shellfish were used...
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PMID: 20549061
PDF is available here.
Abstract
The effectiveness of three natural compounds in slowing down the deterioration of microbial quality of Fior di latte cheese is addressed. In particular, the control of the growth of spoilage microorganisms and the determination of the Microbiological Stability Limit (MAL) were the main goals. A Cent...
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PMID: 20053318
PDF is available here.
Abstract
We analyzed data from 6 phase 3 multinational doripenem clinical trials on ciprofloxacin-resistant Enterobacteriaceae isolates consisting of all genera (CIPRE) and ESBL-producing Enterobacteriaceae isolates consisting of Escherichia coli, Klebsiella spp., and Proteus spp. with ceftazidime MICs of >o...
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PMID: 20211892
PDF is available here.
Abstract
ChromID extended-spectrum beta-lactamase (ESBL) culture medium is routinely used for screening ESBL producers. This medium was tested for detecting carbapenemase-producing Enterobacteriaceae isolates from a collection of reference strains and compared to the CHROMagar KPC culture medium previously e...
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PMID: 20237104
PDF is available here.
Abstract
Many of the Enterobacteriaceae carry multiple drug resistance (MDR) genes on large plasmids of replicon type Inc A/C and Inc H1. It is important to understand the transmission of these MDR plasmids because the genes they carry can affect the outcome of antimicrobial therapy. The aim of this study wa...
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PMID: 20138094
PDF is available here.
Abstract
Cronobacter is a major food-borne pathogen in powdered infant formula and can lead to serious developmental aftereffect and death to infants. The contamination of Cronobacter may be a high risk for the powdered foods. To isolate and identify Korean Cronobacter from the powdered foods such as powdere...
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PMID: 20467249
PDF is available here.
Abstract
A finished compost sample was examined for bacteriocin-like substance production against five pathogenic bacteria: Salmonella typhimurium EF 85-9, Escherichia coli O157:H7 ATCC 43888, Enterococcus faecalis JCM 8726, Staphylococcus aureus JCM 2151, and Yersinia enterocolitica JCM 7577. At the prelimi...
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PMID: 20513963
PDF is available here.
Abstract
We determined the influence of microbial status on global and regional enteric TCRbeta repertoires. The gut of conventionally reared chickens exhibited non-Gaussian distributions of CDR3-lengths with some shared over-represented peaks in neighbouring gut segments. Sequence analysis revealed local cl...
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PMID: 19945480
PDF is available here.
Abstract
We conducted a cohort study to identify risk factors for giardiasis, and stool and environmental samples were analysed. Consuming tap water was significantly associated with illness (risk ratio 4.7, 95% confidence interval 1.5-14.4). Drinking-water samples were coliform-contaminated and a suspect Gi...
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PMID: 19751538
PDF is available here.
Abstract
We analyzed powder infant formula milk (PIF), which may be an important source of infantile infection, regarding contamination with Enterobacteriaceae including this type of bacteria, and conducted drug sensitivity tests with various antimicrobial agents. Enterobacteriaceae was isolated 36 (24.2%) o...
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PMID: 20332571
PDF is available here.
Abstract
The appropriateness of indicator organisms as surrogates for human pathogens in water quality modeling is dependent on similarities in both presence and transport behavior; however, very little data relating indicator and bacterial pathogen transport behavior in receiving waters is available. In thi...
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PMID: 20009247
PDF is available here.
Abstract
We concluded that the inclusion of WB to modify the intestinal microbiota activity combined with LOP may be beneficial to animal health and performance....
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PMID: 19889242
PDF is available here.
Abstract
The distribution of fecal coliforms was investigated and determined in Izmir Bay from 1996 to 2005. Izmir Bay severely was polluted from industrial and domestic discharges during decades. In early 2000, a wastewater treatment plant began to treat domestic and industrial wastes. This plant treats the...
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PMID: 19234749
PDF is available here.
Abstract
Since 2000, Escherichia coli producing CTX-M enzymes (especially CTX-M-15) have emerged worldwide as important causes of community-onset urinary tract infections (UTIs) and bloodstream infections due to extended-spectrum beta-lactamase (ESBL)-producing bacteria. Molecular epidemiology studies sugges...
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PMID: 20166768
PDF is available here.
Abstract
We have retrospectively analyzed the results of 44, 321 urine cultures from January 01, 2008 till June 30, 2009. The study showed that ESBL production was confirmed in 189 (1.8%) of the total of 10,757 isolates. Rates of ESBL-producing isolates were 19%, 0,6%, and 5.2% for Klebisella pneumoniae, Esc...
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PMID: 20387729
PDF is available here.
Abstract
We describe the reliability of the VITEK-2 Compact and overnight MicroScan panels for direct identification and susceptibility testing from the BacT/ALERT blood culture system when using FAN (FA and FN) bottles. A simple procedure, in two centrifugation steps, was designed to remove the charcoal par...
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PMID: 19778301
PDF is available here.