Abstract
We show that RegIIIγ, a secreted antibacterial lectin, is essential for maintaining a ~50-micrometer zone that physically separates the microbiota from the small intestinal epithelial surface. Loss of host-bacterial segregation in RegIIIγ(-/-) mice was coupled to increased bacterial colonization o...
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PMID: 21998396
PDF is available here.
Abstract
This study was conducted to assess inhalation exposure to dust, endotoxin, and microorganisms (including viable bacteria, Gram-negative bacteria [GNB], and fungi) during waste collection and sorting; to identify factors affecting this exposure; and to estimate the gastrointestinal exposure to microor...
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PMID: 21516941
PDF is available here.
Abstract
We have shown previously that spontaneous lateral transfer of non-conjugative plasmids occurs in a colony biofilm of mixed E. coli strains (a set of a donor strain harbouring a plasmid and a plasmid-free recipient strain). In this study, with high-frequency combinations of strains and a plasmid, we...
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PMID: 21283723
PDF is available here.
Abstract
We describe our investigation of early NEC and test the hypothesis that bacteria and inflammatory mediators differ between animals with early NEC and disease free animals.
On DOL7 C3HeB/FeJ pups were fed liquid formula with 1×10(4) Streptococcus thoraltensis, Serratia marcescens, an...
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PMID: 21445365
PDF is available here.
Abstract
INTRODUCTION : Meningitis is the most dreaded cause of morbidity and mortality in neurosurgical patients. The reported incidence of postoperative meningitis is quite varied 0.5-8%. MATERIAL AND METHODS : The study cohort included all the patients who underwent neurosurgery at the department of neuros...
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PMID: 21483116
PDF is available here.
Abstract
We review a selection of recent structural studies which have major ramifications for our understanding of the passage of macromolecules across the outer membrane.
Copyright © 2010 Elsevier Ltd. All rights reserved....
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PMID: 21130656
PDF is available here.
Abstract
Clinical and economic investigation of various antibiotics use in the treatment of complicated urinary tract infection (CUTI) was performed under the Russian economic environment. The drugs of comparison were ertapenem, ceftriaxone and levofloxacin. Direct costs and their structure were shown, and th...
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PMID: 21780670
PDF is available here.
Abstract
This case-controlled study examined clinical and microbiological parameters in Brazilian children and adolescents receiving orthodontic treatment using fixed orthodontic appliances or removable orthodontic appliances.
The plaque index, gingival index, number of decaye...
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PMID: 21344829
PDF is available here.
Abstract
To investigate the bacterial distribution and resistance to antibiotics in culture-positive urine, and to provide reference for rational use of antibiotics in clinical practice.
Distribution and antibiotic resistance to pathogens in 658 culture-positive patients from...
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PMID: 21131743
PDF is available here.
Abstract
We compared the performance of MacConkey agar and Mueller-Hinton agar for metallo-β-lactamase (MBL) and OXA carbapenemase screening. Overall, the performance of Hodge test was better with MacConkey agar due to enhanced release of β-lactamases from the cells in the presence of bile compounds. Conco...
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PMID: 20801167
PDF is available here.
Athina Avlami,
S Bekris,
G Ganteris,
Eleni Kraniotaki,
Eleni Malamou-Lada,
Maria Orfanidou,
Olga Paniara,
Angeliki Pantazatou,
C C Papagiannitsis,
Evangelia Platsouka,
Ioanna Stefanou,
Eva Tzelepi,
Helen Vagiakou and
Vivi Miriagou
Abstract
hyplex®-MBL ID Multiplex PCR-ELISA, a novel method for identifying metallo-β-lactamase genes directly in clinical specimens, was evaluated using a consecutive collection of 326 samples from three hospitals in Greece characterized by high prevalence of VIM producers. The method exhibited high sensi...
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PMID: 20807554
PDF is available here.
Abstract
These results demonstrate that cell wall from MTV can be used to differentially bind E. coli spp. and Salmonella spp. up to 8×10(4) CFU/10 μg. Thus certain yeast cell walls may prevent enteric infections caused by selective bacteria. This methodical approach would be an accurate tool in the feed i...
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PMID: 20826190
PDF is available here.
Abstract
We identified the culturable bacteria forming a biofilm in the microalgal cells by 16S rDNA sequencing. At least eight different culturable species of bacteria were detected in the biofilm and were evaluated for the presence of quorum-sensing signals in these bacteria. Few studies have considered th...
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PMID: 20502890
PDF is available here.
Abstract
Phenotypic and phylogenetic studies were performed on three strains of an acetogenic bacterium isolated from livestock-impacted soil. The bacterium stained Gram-negative and was a non-spore-forming rod that was motile by peritrichous flagella. The novel strains had an optimum pH for growth of 8.0-8....
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PMID: 19965999
PDF is available here.
Abstract
The challenge posed by increasing levels of drug-resistant bacteria world-wide is manifest, and must be dealt with both by new approaches to the use of existing antibiotics and by the introduction of novel drugs. ACHN-490 is the first neoglycoside, or next-generation aminoglycoside, to begin clinica...
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PMID: 20932796
PDF is available here.
Abstract
We expressed and purified the modified BhSGAMP-1-S and investigated its antimicrobial activity.
We synthesized the gene of BhSGAMP-1-S designed with preferred codons of Escherichia coli and expressed it as a fusion protein in E. coli TB1 by using pMAL-c2X as vector. We purified the fusion protein us...
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PMID: 21090174
PDF is available here.
Abstract
We hypothesized that VLBW infants with prior GI tract colonization with gentamicin-susceptible GNB who developed bloodstream infections (BSI) would do so with gentamicin-susceptible GNB.
A prospective cohort study of VLBW infants was performed in 2 level III neonatal intensive care units from Septem...
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PMID: 20539251
PDF is available here.
Abstract
A series of novel 6-O-substituted 8a-aza-8a-homoerythromycin A ketolides was synthesized and evaluated for in vitro antibacterial activity. Key strategic elements of the synthesis include the base-induced E-Z isomerization of 3-O-descladinosyl-6-O-allylerythromycin A 9(E)-oxime followed by ring-expa...
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PMID: 20684614
PDF is available here.
Abstract
Of the 60 patients, 88.3% had monomicrobial infection and 11.7% had mixed infection. The mean duration from the first neurosurgical procedure to the diagnosis of meningitis was 21 days (range, 1-134 days). The median frequency of neurosurgical procedure before meningitis was 1 (range, 1-5). A total...
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PMID: 20688290
PDF is available here.
Abstract
We showed that an Escherichia coli O157:H7 strain that was unable to form biofilm was retained in large numbers in dual-strain biofilms formed with an E. coli O-:H4 companion strain. In this study we tested additional companion strains for their ability to retain E. coli O157:H7 strain 0475s. Compan...
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PMID: 20367070
PDF is available here.
Abstract
Although many antimicrobial components (i.e. antimicrobial peptides) have been found in many social Hymenoptera venoms, no antimicrobial compound is purified and characterized from parasitic Hymenoptera. From the venoms of the ectoparasitic wasp, Nasonia vitripennis, a defensin-like antimicrobial pe...
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PMID: 20362606
PDF is available here.
Abstract
We describe the silver(i) complex of this important biological molecule and show that the binding mode is through the phenoxazine ring nitrogen. A new synthetic route to 2-aminophenoxazine-3-one is also reported....
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PMID: 21072337
PDF is available here.
Abstract
An important risk factor for nosocomial infection in an intensive care unit (ICU) is prior colonization. This study was undertaken to determine the spectrum of bacterial colonization and predisposing risk factors in patients being admitted to an ICU in India, with special emphasis on extended-spectr...
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PMID: 20413621
PDF is available here.
Abstract
Pathogens were isolated from 44% of all patients. MM patients more frequently had multiple pathogens in blood cultures (38% versus 25%). Transplantation related mortality was similar between the groups. CONCLUSION: The frequency of isolated pathogens, positive blood cultures, and the diversity of pa...
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PMID: 20670481
PDF is available here.
Abstract
These results suggest that enzymatic deproteinization of the shrimp shell wastes, using B. cereus SV1 protease, could be applicable to the chitin production process....
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PMID: 19960271
PDF is available here.
Abstract
BACKGROUND AND OBJECTIVIES: Nepeta species are used as diuretic, diaphoretic, antitussive, antispasmodic, antiasthmatic, febrifuge, emmenagogue, sedative agents, insecticidal, acaricidal, antiviral, anti-inflammatory and antioxidant. Acetone extract of Nepeta meyeri (Labiatae) was screened for antio...
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PMID: 20707285
PDF is available here.
Abstract
Gram-negative endocarditis due to HACEK bacteria (Haemophilus species, Actinobacillus, Cardiobacterium, Eikenella and Kingella species) and non-HACEK organisms is an infrequent occurrence but is associated with significant morbidity and mortality. Traditionally, non-HACEK Gram-negative endocarditis...
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PMID: 20695743
PDF is available here.
Abstract
This study aimed to assess the prevalence of seven periodontal marker pathogens, before implant placement and 1 yr after loading, in periodontally healthy individuals and to assess the long-term effectiveness of pre-implant reduction of pathogens to below threshold levels. In 93 individuals needing...
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PMID: 20662908
PDF is available here.
Abstract
We developed an in vivo method to detect the orientation of outer membrane cytochromes via analysis of electron transfer reactions between these enzymes and riboflavin....
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PMID: 20451567
PDF is available here.
Abstract
Manufacturers of dental units have responded positively to the challenge from the American Dental Association (ADA) and the subsequent guidelines issued by the Centers for Disease Control and Prevention (CDC) to deliver patient treatment water that is at least as pure as drinking water. Dental units...
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PMID: 20737988
PDF is available here.
Abstract
We report the biosynthesis of extracellular silver-based single nanocrystallites of well-defined composition and homogeneous morphology utilizing the gamma-proteobacterium, Shewanella oneidensis MR-1, upon incubation with aqueous silver nitrate solution. Further characterization of these particles r...
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PMID: 20509652
PDF is available here.
Abstract
Different disinfecting solutions used during storage in cases result in different levels of contamination and contamination by different types of microbes. These differences are not simply because of the types of disinfectants used, suggesting that other excipients in, or formulation of, the solutio...
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PMID: 20436374
PDF is available here.
Abstract
Antibiotic resistance development is an ongoing process associated with irrational antibiotic use. WHO recommends regular surveillance programs for monitoring of antibiotic resistance. The present study is a step in this direction. A total of 124 clinical isolates of Escherichia coli, Staphylococcus...
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PMID: 20566434
PDF is available here.
Abstract
For the ciprofloxacin 400 mg intravenously every 8 h regimen, the 24-h AUC/MIC at MICs of 0.5 and 1 microg/ml were 218.63 +/- 78.75 and 109.31 +/- 39.37, respectively. For the ciprofloxacin 400 mg intravenously every 12 h regimen, the 24-h AUC/MIC at MICs of 0.5 and +/- microg/ml were 144.07 +/- 57....
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PMID: 20649056
PDF is available here.
Abstract
Although Gram-negative bacteria are the most common etiology of fever in neutropenic patients, the occurrence of infections caused by coagulase-negative Staphylococcus and molds has been increasing in comparison with the observations from previous studies in King Chulalongkorn Memorial Hospital. To...
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PMID: 20649055
PDF is available here.
Abstract
We will summarize recent understanding of the molecular mechanisms contributing to the RK2 DNA replication and partitioning.
Copyright © 2010 Elsevier Inc. All rights reserved....
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PMID: 20600283
PDF is available here.
Abstract
Even though recurrent gram-negative BSI was relatively uncommon in the general population, up to 15% of patients with gram-negative BSI developed a recurrent episode within 10 years of the initial episode.
Copyright (c) 2010 The British Infection Society. Published by Elsevier Ltd. All rights reserv...
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PMID: 20378069
PDF is available here.
Abstract
The epicarp and seed of Persea Americana Mill. var. Hass (Lauraceae), Persea Americana Mill. var. Shepard, and Persea americana Mill. var Fuerte cultivars of mature avocados (n = 3) were ground separately and extracted with both absolute ethanol and distilled water. Extracts were analyzed for antimi...
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PMID: 20645772
PDF is available here.
Abstract
We have investigated the antibacterial activity of some new steroidal thiosemicarbazones and their Pd(II) metal complexes were prepared by the reaction of the thiosemicarbazones with [Pd(DMSO)(2)Cl(2)]. The steroidal thiosemicarbazones were prepared by the reaction of thiosemicarbazides with a stero...
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PMID: 20657393
PDF is available here.
Abstract
All cells must traffic proteins into and across their membranes. In bacteria, several pathways have evolved to enable protein transfer across the inner membrane, the periplasm, and the outer membrane. The major route of protein translocation in and across the cytoplasmic membrane is the general secr...
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PMID: 20204450
PDF is available here.
Abstract
Conditions that sustain constant bacterial growth are seldom found in nature. Oligotrophic environments and competition among microorganisms force bacteria to be able to adapt quickly to rough and changing situations. A particular lifestyle composed of continuous cycles of growth and...
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PMID: 20236330
PDF is available here.
Abstract
A total of 594 nonurinary Gram-negative isolates were examined. Susceptibility testing was performed for 270 (45.4%) Enterobacteriaceae, 209 (35.2%) Gram-negative nonfermentative bacilli and 115 (19.4%) other Gram-negative bacteria. In total, 385 (64.8%) were susceptible to fosfomycin. Specifically,...
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PMID: 20521939
PDF is available here.
Abstract
The study of biochemical activity of extracts obtained from marine organisms is gaining interest as some have proved to have efficient health or industrial applications. To evaluate lethality and antimicrobial activities, some chemical tests were performed on crude extracts of the octocorals Eunicea...
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PMID: 20527468
PDF is available here.
Abstract
A commercial duck company that raises approximately two million Pekin ducks per year experienced an outbreak of Riemerella anatipestifer (RA) on nine farms over a 1-yr period. Owing to concerns that the bacteria was being spread from farm to farm, an investigation using serotyping and DNA fingerprin...
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PMID: 20608551
PDF is available here.
Abstract
These results extend the list of promising isothioureas with substantial activity in vitro and suggest that an in-depth study of toxicity, antimicrobial properties in vivo and nitric oxide synthase isoform selectivity of selected novel compounds is warranted....
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PMID: 20607486
PDF is available here.
Abstract
GNB accounted for the majority of bloodstream infections at our institute, unlike data from developed countries. Uptake of the new FN protocol was satisfactory, although the use of oral antibiotics as fi rst-line empirical therapy can be improved. A better method for predicting infections caused by...
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PMID: 20625621
PDF is available here.
P Durando,
M Bassetti,
G Orengo,
P Crimi,
A Battistini,
G Tiberio,
D Bellina,
A Talamini,
F Dodi,
F Ansaldi,
C Alicino,
R Iudici,
L Sticchi,
D De Florentiis,
C Viscoli,
G Icardi and
Prevalence Study Working Group of the San Martino University Hospital of Genoa
Abstract
A prevalence study aimed to update the epidemiological scenario of Hospital-Acquired Infections (HAI) was performed at the San Martino University Hospital of Genoa, the Regional Reference Adult-care Center in Liguria, Italy, with more than 1300 beds.
The investigation was performed in all the wards,...
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PMID: 21155410
PDF is available here.
Abstract
Filarial nematode species can host Wolbachia bacterial endosymbionts. To understand the symbiosis, a higher level of complexity should be considered, taking in account the tripartite association between Wolbachia, filariae and mammals. This overview article discusses the biology of Wolbachia in fila...
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PMID: 20597434
PDF is available here.
Abstract
The diversity and antifungal resistance of yeasts able to grow at 37 degrees C and the occurrence of bacterial indicators of water quality were studied in three lakes in Southeastern Brazil. The densities of yeasts, Escherichia coli, Enterococcus spp. and Pseudomonas aeruginosa were determined by th...
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PMID: 20154391
PDF is available here.
Abstract
As little is known about antimicrobial resistance genes in fish farms, this study was conducted to monitor the incidence and prevalence of a wide range of antimicrobial resistance genes in Gram-negative bacteria isolated from water samples taken from fish farms in the northern part of Egypt. Ninety-...
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PMID: 20145377
PDF is available here.
Abstract
A quadruplex real-time (RT) qPCR assay for the detection and quantification in 4h of Staphylococcusaureus, Pseudomonasaeruginosa, Acinetobacterbaumannii and Stenotrophomonasmaltophilia directly from bronchoalveolar lavage specimens was developed. The specificity of the assay was 100% for all four sp...
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PMID: 20353803
PDF is available here.
Abstract
We obtained an accurate measurement of the effective charge density of LPS monolayers. The experimental findings were compared to the results of Monte Carlo simulations based on a coarse-grained minimal model of LPS molecules and showed excellent agreement....
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PMID: 20442333
PDF is available here.